| distribution of ferritin-conjugated lectins on sialidase-treated membranes of human erythrocytes. | the labeling of sialidase-treated, human erythrocyte membranes with ferritin-conjugates of four plant lectins, concanavalin a, ricinus communis hemagglutinin, bauhinia purpurea hemagglutinin and arachis hypogoea hemagglutinin, is reported. among these ferritin-conjugated lectins, ferritin-conjugated concanavalin a and ferritin-conjugated r. communis hemagglutinin were found in clusters on the sialidase-treated membranes, whereas ferritin-conjugated b. purpurea hemagglutinin and ferritin-conjugat ... | 1975 | 172150 |
| syntheses of o-beta-d-galactopyranosyl-(1 leads to 3)-0-(2-acetamido-2-deoxy-alpha(and -beta)-d-galactopyranosyl)-n-tosyl-l-serine and their interaction with d-galactose-binding lectins. | in order to use, as hapten inhibitors against various galactose-binding lectins, the derivatives of o-beta-d-galactopyranosyl-(1 leads to 3)-o-(2-acetamido-2-deoxy-alpha-d-galactopyranosyl)-l-serine, which is the common core structure of sugar chains of most mucins, the synthesis of these compounds was investigated. koenigs-knorr condensation of the 4,6-o-benzylidene derivative of o-(2-acetamido-2-deoxy-alpha-d-galactopyranosyl)-n-tosyl-l-serine methyl ester with 2,3,4,6-tetra-o-acetyl-alpha-d-g ... | 1979 | 427833 |
| age-related cell surface changes in human diploid fibroblasts revealed by lectin-mediated red blood cell adsorption assay: a lectin survey. | two types of age-related cell surface changes could be demonstrated in human diploid fibroblasts with the two methods of the lectin-mediated red blood cell (rbc) adsorption assay: the fibroblast coating method (in which rbcs are adsorbed to lectin-coated fibroblasts) and the rbc coating method (in which lectin-coated rbcs are adsorbed to fibroblasts). with the fibroblast coating method, concanavalin a and agglutinin l from phaseolus vulgaris gave a change in rbc adsorption which did not occur th ... | 1979 | 522510 |
| elucidation of lectin receptors by quantitative inhibition of lectin binding to human erythrocytes and lymphocytes. | the binding to normal and sialidase-treated human erythrocytes and lymphocytes of four 125i-labeled lectins [maackia amurensis hemagglutinins (mam and mah), ricinus communis hemagglutinin (rch), and bauhinia purpurea hemagglutinin (bph)] was studied in detail. the quantitative inhibition assays against the lectin binding to the cells were also performed with various glyco-proteins and glycopeptides as inhibitors. the comparison of the inhibition constants of the inhibitors thus obtained with the ... | 1976 | 974078 |
| immunochemical and chemical investigations of the structure of glycoprotein fragments obtained from epiglycanin, a glycoprotein at the surface of the ta3-ha cancer cell. | the structures of the carbohydrate chains present in fragments of a large-molecular-weight glycoprotein, epiglycanin, cleaved from the surface of viable ta3-ha murine mammary carcinoma ascites cells and purified by gel filtration, were studied by immunochemical and chemical methods. inhibitory activities for neuraminidase-treated and untreated glycoprotein material in the hemagglutination of nn-specific human erythrocytes by eight purified lectins were determined. excellent inhibition was obtain ... | 1975 | 1125949 |
| further characterization of some heterophile agglutinins reacting with alkali-labile carbohydrate chains of human erythrocyte glycoproteins. | the nature of the receptor sites for several agglutinins is characterized by hemagglutination inhibition assays. the inhibitory activity of human erythrocytes glycoproteins, from which sialic acid, sialic acid and galactose or alkali-labile oligosaccharides have been removed, is compared to the inhibitory effect of compounds with known structure. it is shown that the lectin from arachis hypogea and anti-t bind to alkali-labile galactosyl-residues. agglutinins from bauhinia purpurea and variegata ... | 1975 | 1172890 |
| clinical evaluation of pancreatic cancer-associated mucin expressing ca19-9, ca50, span-1, sialyl ssea-1, and dupan-2. | we have previously described the purification and partial characterization of a new pancreatic cancer-associated antigen, a pancreatic cancer-associated mucin expressing ca19-9, ca50, span-1, sialyl ssea-1, and dupan-2. this study describes the clinical evaluation of various assay systems for this antigen which depend on measuring respective serum levels. elevated levels of antigen were detected in the sera from both patients with malignant and non-malignant diseases. however, elevated serum lev ... | 1992 | 1359630 |
| interaction of lectins with human ige: ige-binding property and histamine-releasing activity of twelve plant lectins. | we examined the ige-binding reaction and the histamine-releasing response of basophils to a panel of 12 lectins: concanavalin a (con a), lens culinaris hemagglutinin (lch), pisum sativum agglutinin (psa), wheat germ agglutinin (wga), soybean agglutinin (sba), bauhinia purpurea agglutinin (bpa), peanut agglutinin (pna), ricinus communis agglutinin i (rca-i), lotus tetragonolobus agglutinin (lotus a), ulex europeus agglutinin i (uea-i), phytohemagglutinin e (pha-e) and phytohemagglutinin l (pha-l) ... | 1992 | 1378039 |
| serological and biochemical studies on en(a-) human erythrocytes in a japanese family. | the propositus erythrocytes with phenotype en(a-), which was found in the first example of a japanese family, reacted with anti-n serum weaker than the ordinary phenotype n erythrocytes. when the erythrocyte membranes of the propositus were subjected to sds-page, no glycophorin a was observed on the gel by pas staining, whereas glycophorin b band was observed. the s and the s antigens of the propositus erythrocytes were appeared to be normal. these results suggested that n activity of the propos ... | 1992 | 1460794 |
| determination of the carbohydrate-binding site of bauhinia purpurea lectin by affinity chromatography. | to determine the carbohydrate-binding site of bauhinia purpurea lectin (bpa), a d-galactose- and lactose-binding lectin, a peptide which interacts with lactose was purified from endoproteinase asp-n digests of bpa by chromatography on a lactose-sepharose column. it consists of nine amino acids and its amino acid sequence is asp-thr-trp-pro-asn-thr-glu-trp-ser. a tryptic fragment with the ability to interact with lactose was also purified and found to contain this sequence, consisting of nine ami ... | 1992 | 1517321 |
| flow cytometric analyses of lectin binding to pneumocystis carinii surface carbohydrates. | pneumocystis carinii obtained from infected rat lung homogenates was incubated with fluorescein isothiocyanate-conjugated lectins, counterstained with the nuclear stain, propidium iodide (pi), and analyzed by dual parameter histograms for lectin-associated green and pi-associated red fluorescence using a fluorescence-activated cell sorter. the presence of glucose/mannose moieties was evidenced by the binding of all organisms to concanavalin a and wisteria floribunda. from the lectin group specif ... | 1992 | 1556643 |
| alteration of the carbohydrate-binding specificity of the bauhinia purpurea lectin through the preparation of a chimeric lectin. | a chimeric lectin gene was constructed by using a cdna clone coding the bauhinia purpurea lectin (bpa) in which a part of the metal-binding region was replaced by the corresponding region of the mannose-binding lens culinaris lectin (lca). the chimeric lectin expressed in escherichia coli was found to bind alpha mannosyl-bovine serum albumin (bsa) and this binding was inhibited by mannose. | 1992 | 1607368 |
| cdna cloning and expression of bauhinia purpurea lectin. | bauhinia purpurea lectin (bpa) was purified from seeds of b. purpurea alba. the purified lectin was digested with an endoproteinase, asp-n, or trypsin and then the amino acid sequences of the resultant fragments were analyzed. furthermore, a cdna library for bpa was constructed using rna isolated from germinated bauhinia purpurea seeds. by gene cloning, the nucleotide sequence of bpa cdna and its deduced amino acid sequence were analyzed. the cloned bpa cdna comprised 1,152 nucleotides and the o ... | 1991 | 1657898 |
| preparation of pancreatic cancer-associated mucin expressing ca19-9, ca50, span-1, sialyl ssea-1, and dupan-2. | a new antigen associated with pancreatic cancer was prepared by immunoaffinity chromatography using fab'-sepharose beads. this antigen was a glycoprotein of large molecular weight (mr greater than 8,000,000) in its native state, estimated by size exclusion chromatography on sephacryl s400. after sodium dodecyl sulfate-polyacrylamide gel electrophoresis and blotting analysis, several cancer-associated glycoconjugates, including ca19-9, ca50, span-1, dupan-2, and sialyl ssea-1, were detected on th ... | 1991 | 1682994 |
| a 58-kda resident protein of the cis golgi cisterna is not terminally glycosylated. | a 58-kda golgi protein (gp58) was previously identified and found to be concentrated in cis golgi cisternae in several cell types (saraste, j., palade, g.e., and farquhar, m.g. (1987) j. cell biol. 105, 2021-2029). in this study the protein was partially purified from rat pancreas and mouse myeloma cells in order to characterize its oligosaccharides. it migrated on sodium dodecyl sulfate-polyacrylamide gels as a 57-58-kda doublet under reducing conditions or as a single approximately 116-kda ban ... | 1991 | 1894639 |
| species identification and characterization of an acanthamoeba strain from human cornea. | the isoenzyme pattern of an acanthamoeba, stock h-1, isolated from a patient with keratitis (krankenhaus heidberg, hamburg) was compared with that of two strains of a. quina-a. lugdunensis (302-2, 312-1), two stocks of a. lenticulata (45, 89-1) and one strain of a. rhysodes (302-1). the isolated stock showed glucose-6-phosphate dehydrogenase (g-6-pdh), beta-hydroxybutyric dehydrogenase (beta-hbdh), alcohol dehydrogenase (adh) and superoxide dismutase (sod) isoenzyme patterns similar to those of ... | 1991 | 1924251 |
| characterization of galactose-containing glycoconjugates in the human retina: a lectin histochemical study. | seven specimens of morphologically normal formalin-fixed and paraffin-embedded human retina were studied using a panel of fourteen biotinylated lectins, all of which react with glycoconjugates containing galactose and n-acetylgalactosamine residues. agaricus bisporus (aba), bauhinia purpurea (bpa), phaseolus vulgaris (pha-e), peanut (pna) and ricinus communis (rca-i) agglutinins labeled photoreceptor cells prior to enzymatic predigestion. bpa and pna bound specifically to cones. the plexiform la ... | 1990 | 2091899 |
| lectin typing of campylobacter isolates. | isolates of campylobacter jejuni, c coli, c fetus and c laridis were tested for agglutination reactions with a panel of five lectins: arachis hypogaea, bauhinia purpurea, solanum tuberosum, triticum vulgaris and wisteria floribunda. twenty three patterns of agglutination (lectin types) were recorded among 376 isolates. patterns were consistent and reproducible. only 4.5% of isolates were untypable because of autoagglutination. some lectin types were found exclusively or predominantly in a specie ... | 1990 | 2262570 |
| expression of cell surface sialic acid and galactose by normal adult human melanocytes in culture. | normal adult human melanocytes grown either in the presence of phorbol ester or dialyzed hypothalamic extract were analyzed for their cell surface sialic acid and galactose content. in both cases, cells expressed large amounts of sialic acid, whereas they differed in their terminal nonreducing beta-d-galactosyl residues linked to n-acetyl galactosamine; such residues were accessible to peanut agglutinin and bauhinia purpurea lectin on cells grown in phorbol ester and inaccessible on cells grown ... | 1990 | 2385567 |
| differentiation of rodent trypanosomes of the subgenus herpetosoma by lectins. | trypomastigote forms of trypanosoma microti, t. evotomys, t. grosi, t. musculi, and t. lewisi and trypomastigote and epimastigote forms of t. acomys were differentiated using 34 lectins and the aminoff test for n-acetylneuraminic acid (nana). twelve of the lectins failed to agglutinate any of the above species. the number of lectins which agglutinated each species differed; t. mciroti, t. evotomys, t. grosi, t. musculi, t. lewisi and t. acomys (trypomastigote and epimastigote forms) were aggluti ... | 1989 | 2617032 |
| demonstration of sugar residues in the ultimobranchial tubule and thyroid c-cells of the rat using peroxidase labelled lectins. | the distribution of various sugars, that contribute to the composition of the carbohydrate components of the glycoprotein in the ultimobranchial tubule and the thyroid c-cells has been studied, using eight peroxidase-labelled lectins (dolichos biblorus, glycine max, bauhinia purpurea, helix pomatia, arachis hipogaea, ulex europaeus i, limax flavus and sophora japonica). the results obtained seem to show that there are receptors for dolichos biflorus, glycine max, bauhinia purpurea, helix pomatia ... | 1989 | 2757235 |
| lectins as probes to pneumocystis carinii surface glycocomplexes. | the binding characteristics of a panel of commercially available fitc-conjugated lectins to pneumocystis carinii (pc) were assessed by fluorescence microscopy and flow cytometry. rat pc obtained from infected lung homogenates were incubated with fitc-conjugated lectins in a series of concentrations, counterstained with propidium iodide, and analyzed for percent fluorescence and fluorescence intensity. all organisms bound concanavalin a and wisteria floribunda agglutinin, 2 representatives of the ... | 1989 | 2785201 |
| studies of lectin binding to the human cervix uteri: i. normal cervix. | lectins of bauhinia purpurea (bpa), canavalin ensiformis (con a), griffonia simplicifolia i (gs i), griffonia simplicifolia ii (gs ii), maclura pomifera (mpa), arachis hypogaea (pna), glycine max (sba), ulex europaeus i (uea i) and triticum vulgaris (wga) were used to evaluate cell surface carbohydrates in formalin-fixed paraffin-embedded tissue sections of normal human cervix uteri. consistent patterns of staining of the squamous epithelium were obtained in all 30 cases with bpa, gs ii, mpa, pn ... | 1989 | 2793521 |
| studies of lectin binding to the human cervix uteri: ii. cervical intraepithelial neoplasia and invasive squamous carcinoma. | the cell surface carbohydrate profile of formalin-fixed paraffin-embedded tissue sections of neoplastic cervical squamous epithelium was evaluated using lectins of bauhinia purpurea (bpa), canavalin ensiformis (con a), griffonia simplicifolia i (gs i), griffonia simplicifolia ii (gs ii), maclura pomifera (mpa), archis hypogaea (pna), glycine max (sba), ulex europaeus i (uea i) and triticum vulgaris (wga). three lectins (bpa, con a and pna) showed a similar pattern of staining in both normal squa ... | 1989 | 2793522 |
| a 160-kilodalton epithelial cell surface glycoprotein recognized by plant lectins that inhibit the adherence of actinomyces naeslundii. | the adherence of actinomyces naeslundii to human epithelial (kb) cells is mediated by the interaction of a fimbrial lectin on this oral bacterium with epithelial cell receptors exposed by sialidase. the d-galactose- and n-acetyl-d-galactosamine-reactive plant lectins from peanut and from bauhinia purpurea inhibit this interaction. this report describes the partial purification and characterization of a 160-kilodalton (kda) cell surface glycoprotein which is the principal receptor for these lecti ... | 1986 | 2872166 |
| molecular basis of bacterial adhesion in the oral cavity. | the two varieties of fimbriae identified on oral strains of actinomyces have distinct functional properties. the type 1 fimbriae of actinomyces viscosus t14v mediate attachment to saliva-treated hydroxyapatite. type 2 fimbriae--on a. viscosus and the only fimbriae detected on a. naeslundii wvu45--are associated with lectin activity. interaction of these fimbriae with complementary receptors initiates bacterial attachment to streptococcus sanguis 34 and sialidase-treated epithelial cells and the ... | 1987 | 2891180 |
| comparative investigations of latin american trypanosomes with lectins and complement lysis test. | four-days old epimastigote culture forms of different stocks of trypanosoma cruzi from colombia, costa rica and mexico as well of colombian stocks of t. rangeli were tested with 27 lectins and the complement lysis test. while the stocks of t. cruzi and t. rangeli showed common agglutination reactions with the lectins of canavalia ensiformis and pisum sativum, only the stocks of t. cruzi were additionally agglutinated by ricinus communis-120, glycine maxima, helix pomatia, axinella polypoides, ba ... | 1986 | 3085200 |
| detection of surface carbohydrates on pneumocystis carinii by fluorescein-conjugated lectins. | lectins react with a wide range of different carbohydrates (table 1). even so-called monospecific anti-h(o) lectins from lotus tetragonolobus, ulex europaeus, and anguilla anguilla react not only with the anti-h determinant but also with several fucosylated carbohydrates. consequently, the type of lectin receptor existing on the surface of pneumocystis carinii should be determined, because only a carbohydrate analysis can demonstrate the kind of carbohydrates which exist on the cell surface of t ... | 1987 | 3125541 |
| pneumocystis carinii: surface reactive carbohydrates detected by lectin probes. | pneumocystis carinii obtained from rat lungs (rlh) and in vitro culture (rtc) were reacted with a panel of 14 fluorescein isothiocyanate conjugated lectins. percentage fluorescence and fluorescent intensity were determined for both trophic and cyst forms. all rlh and rtc derived organisms bound strongly concanavalin a (con a), and wheat germ agglutinin (wga). however, differences in soybean agglutinin (sba) binding between rlh and rtc organisms was observed. different subsets of the organism bou ... | 1988 | 3142787 |
| specific and nonspecific inhibition of adhesion of oral actinomyces and streptococci to erythrocytes and polystyrene by caseinoglycopeptide derivatives. | various caseinoglycopeptide derivatives prepared from mammalian milk were evaluated as inhibitors of hemagglutinations mediated by actinomyces viscosus ny1, streptococcus sanguis omz9, and, for comparative purposes, plant lectins from arachis hypogaea and bauhinia purpurea. it was found that recognition of the beta-d-galactose-(1----3)-2-acetamido-2-deoxy-d-galactose carbohydrate chain by actinomyces viscosus ny1 organisms and arachis hypogaea and b. purpurea agglutinins had similar structural r ... | 1988 | 3182077 |
| carbohydrate-binding specificities of five lectins that bind to o-glycosyl-linked carbohydrate chains. quantitative analysis by frontal-affinity chromatography. | the carbohydrate-binding specificities of lectins purified from agaricus bisporus (aba-i), arachis hypogaea (pna), bauhinia purpurea (bpa), glycine max (sba), and vicia villosa (vva-b4) have been studied by affinity chromatography on columns of the immobilized lectins, and quantitatively analyzed by frontal affinity chromatography. these five lectins could be classified into two groups with respect to their reactivities with typical mucin-type glycopeptides, beta-d-galp-(1----3)-alpha-d-galpnac- ... | 1988 | 3274131 |
| glycophorin is the reovirus receptor on human erythrocytes. | purified glycophorin (predominantly type a) from human erythrocytes was found to effectively inhibit reovirus hemagglutination (ha) in contrast to other glycoproteins such as fetuin or ovalbumin. glycophorin was also a potent inhibitor of reovirus and protein sigma 1 binding to mouse l fibroblasts. glycophorin pretreated with neuraminidase lost these inhibitory properties. using a solid phase binding assay, it was demonstrated that reovirus as well as protein sigma 1 could specifically bind to g ... | 1987 | 3604060 |
| pregnancy-related changes in the human endometrium revealed by lectin histochemistry. | the binding of 22 fluorescein isothiocyanate (fitc) conjugated lectins to human proliferative phase and pregnant endometrium was studied histochemically. only the lectin from bauhinia purpurea (bpa) reacted exclusively with the epithelial cells. all the others reacted to a certain extent with glandular and/or stromal cells. lectins from soybean (sba), and vicia villosa seeds (vva) reacted with endometrial glands of pregnancy but not with the glands of the proliferative endometrium. in the prolif ... | 1985 | 3997559 |
| assignment of lectins specific for d-galactose or n-acetyl-d-galactosamine to two groups, based on their circular dichroism. | the circular dichroism (cd) spectra of thirteen lectins, most being specific for d-galactose or n-acetyl-d-galactosamine, were compared. two groupings are proposed on the basis of the cd in the near-ultraviolet region. group one comprises the lectins from arachis hypogaea, glycine max, phaseolus coccineus, p. lunatus, p. vulgaris, and vicia villosa, and group two comprises lectins from caragana arborescens, cytisus sessilifolius, dolichos biflorus, griffonia simplicifolia, and wisteria floribund ... | 1985 | 4016573 |
| reactivity of five n-acetylgalactosamine-recognizing lectins with preimplantation embryos, early postimplantation embryos, and teratocarcinoma cells of the mouse. | the expression of receptors for n-acetylgalactosamine-recognizing lectins, namely helix pomatia agglutinin (hpa), sophora japonica agglutinin (sja), bauhinia purpurea agglutinin (bpa), vicia villosa agglutinin (vva), and wistaria floribunda agglutinin (wfa) was studied in early mouse embryos and teratocarcinoma cells. each of these lectins as well as dolichos biflorus agglutinin (dba) bound differently to early embryonic cells, with the exception of vva and wfa which showed indistinguishable rea ... | 1985 | 4018458 |
| studies on lectins with a broad agglutination spectrum. xii. n-acetyl-d-galactosamine specific lectins from the seeds of soja hispida, bauhinia purpurea, iberis amara, moluccella laevis and vicia graminea. | | 1971 | 5165766 |
| lectin-dependent attachment of actinomyces naeslundii to receptors on epithelial cells. | the adherence of actinomyces naeslundii wvu45 to monolayer cultures of human epithelial cell lines was mediated by the lactose-sensitive fimbriae (type 2) of strain wvu45. the attachment of actinomyces viscosus t14v, which has both types 1 and 2 fimbriae, was approximately half that of a. naeslundii, and only minimal attachment of a. naeslundii and a. viscosus mutants lacking type 2 fimbriae was detected. the adherence of strain wvu45 was enhanced two- to threefold by neuraminidase treatment of ... | 1984 | 6150008 |
| enrichment of il-2-producer t cells from mouse spleen by use of bauhinia purpurea lectin. | bauhinia purpurea agglutinin (bpa) was used to enrich the interleukin 2 (il 2)-producing t-cell subset from mouse spleen. il 2 was found to be produced by the bpa-non-agglutinated (bpa-) t cells of mouse spleen on stimulation with concanavalin a (con a). the degree of il 2 production by bpa-agglutinated (bpa+) t cells was significantly low. the proliferative response to con a of bpa- t cells was not influenced by the addition of exogenous il 2, whereas bpa+ t cells were partially dependent on th ... | 1983 | 6226088 |
| pregnancy-related changes in the mouse oviduct and uterus revealed by differential binding of fluoresceinated lectins. | the binding of 20 fluorescein isothiocyanate (fitc)-labeled lectins to various portions of the pregnant and non-pregnant murine oviduct and uterus was studied by fluorescence microscopy. five lectins (from ricinus communis (rca-i), maclura pomifera (mpa), triticum vulgare (wheat germ-wga), bauhinia purpurea (bpa), and ulex europeus (uea-i] reacted differentially with the epithelium of pregnant as compared with the non-pregnant uterus. the binding of rca-i, mpa and wga delineated pregnancy-relate ... | 1983 | 6418694 |
| the reactivities of bauhinia purpurea and lens culinaris lectins to mouse b lymphocytes and their subsets. | the use of lectins for the separation of murine spleen lymphocyte subsets was examined. bauhinia purpurea agglutinin was found to be effective for the enrichment of b cells by differential agglutination. when the differential agglutination technique using lectins was applied to the separation of b cell subsets, lens culinaris agglutinin was found to be able to fractionate b cells into high responding and low responding subsets for dextran sulfate. a different expression of cell surface igd was a ... | 1983 | 6605940 |
| presence in mouse bone marrow of mediator-dependent cytotoxic effector cells against tumors. | the role of bone marrow cells as cytotoxic effector cells against tumor cells was examined in vitro. bone marrow cells from normal c3h/he mice could lyse murine mm46 tumor cells in the presence of wheat germ agglutinin or syngeneic antitumor antibody. bone marrow cells alone did not cause lysis. eleven other lectins (concanavalin a, phytohemagglutinin, pokeweek mitogen, lens culinaris hemagglutinin, ulex europaeus agglutinin, peanut agglutinin, bandeiraea simplicifolia agglutinin, bauhinia purpu ... | 1983 | 6689597 |
| alterations in human colonic mucin occurring with cellular differentiation and malignant transformation. | the binding of fluorescein isothiocyanate (fitc)-conjugated lectins to mucin in the human colon was studied by using fluorescence microscopy. in normal mucosa, lectins that preferentially bind to exposed n-acetyl-galactosamine residues (dolichos biflorus agglutinin and soybean agglutinin) bound selectively to the goblet cell mucin of well-differentiated cells in the upper colonic crypt. by contrast, lectins that require exposed non-reducing galactose residues for binding (ricinus communis agglut ... | 1982 | 6952252 |
| characterization of lectin-induced cellular cytotoxicity mediated by mouse spleen cells and the role of lymphotoxin. | the cellular cytotoxicity mediated by mouse spleen cells in the presence of mitogenic or non-mitogenic lectins was established under serum-free conditions and characterized. compared with the lectin-induced cellular cytotoxicity (licc) in the guinea-pig, the activity of lymphotoxin (lt) released into the murine licc assay cultures was very low. however, a positive correlation was found between the strength of licc and the lt activity released into the supernatants. moreover, the addition of puro ... | 1980 | 6970163 |
| lectin receptors as markers for trypanosoma cruzi. developmental stages and a study of the interaction of wheat germ agglutinin with sialic acid residues on epimastigote cells. | trypanosoma cruzi at various stages of maturation and differentiation have been isolated by conventional cellular fractionation procedures and characterized by cell surface markers using 30 highly purified lectins encompassing all known sugar specificities. cell surface carbohydrates of the various t. cruzi stages were analyzed by agglutination and lectin-binding assays. specific receptors for wheat germ agglutinin (wga), helix pomatia, sophora japonica, and bandeiraea simplicifolia lectin ii we ... | 1980 | 7000967 |
| binding-site specificity of lectins from bauhinia purpurea alba, sophora japonica, and wistaria floribunda. | | 1980 | 7438131 |
| immunochemical studies on the combining site of the d-galactopyranose and 2-acetamido-2-deoxy-d-galactopyranose specific lectin isolated from bauhinia purpurea alba seeds. | | 1980 | 7447466 |
| interaction of native and asialo rat sublingual glycoproteins with lectins. | the binding properties of the rat sublingual glycoprotein (rsl) and its asialo product with lectins were characterized by quantitative precipitin(qpa) and precipitin inhibition(qpia) assays. among twenty lectins tested for qpa, native rsl reacted well only with artocarpus integrifolia (jacalin), but weakly or not at all with the other lectins. however, its asialo product (asialo-rsl) reacted strongly with many gal and galnac specific lectins-it bound best to three of the galnac alpha 1-->ser/thr ... | 1995 | 7475931 |
| inhibition of herpes simplex, rabies and rubella viruses by lectins with different specificities. | a series of eleven lectins were investigated for their inhibiting activity towards herpes simplex type 1, rabies and rubella viruses. herpes simplex virus adsorption was inhibited by limulus polyphemus (lpa), canavalia ensiformis (cona) and wheat germ (wga) agglutinins, whereas bauhinia purpurea (bpa) and soybean agglutinins were more effective if present during viral replication; rabies virus attachment to susceptible cells was prevented by lpa and narcissus pseudonarcissus (npa) agglutinin whe ... | 1995 | 7481093 |
| lectin and immunohistochemical comparison of glycoconjugates in the conjunctiva of patients with and without exfoliation syndrome. | aims--the study was carried out to search for labelling similar to that of intraocular exfoliation material in the conjunctiva by light microscopy using lectin and immunohistochemistry. methods--ten formalin fixed and paraffin embedded conjunctival biopsy specimens both from patients with and without exfoliation syndrome were studied with a panel of 11 lectins and with three monoclonal antibodies to the hnk-1 carbohydrate epitope, all of which react with intraocular exfoliation material. results ... | 1995 | 7542024 |
| putative glycoprotein and glycolipid polymorphonuclear leukocyte receptors for the actinomyces naeslundii wvu45 fimbrial lectin. | recognition of receptors on sialidase-treated polymorphonuclear leukocytes (pmns) by the gal/galnac lectin associated with the type 2 fimbriae of certain strains of actinomyces results in activation of the pmns, phagocytosis, and destruction of the bacteria. in the present study, plant lectins were utilized as probes to identify putative pmn receptors for the actinomyces lectin. the gal-reactive lectin from ricinus communis (rcai), the gal/galnac-reactive lectins from r. communis (rcaii) and bau ... | 1995 | 7790078 |
| galactose-containing glycoconjugates of the iris, the aqueous outflow passages and the cornea in capsular glaucoma. a lectin histochemical study. | the aim of the study was to compare galactose-containing glycoconjugates of the iris, the aqueous outflow passages and the cornea with exfoliation material in capsular glaucoma. | 1995 | 7797082 |
| bauhinia purpurea lectin (bpa) binding of rat type i pneumocytes: alveolar epithelial alterations after radiation-induced lung injury. | in the rat lung, we found that the bauhinia purpurea lectin (bpa) specifically binds to the type i alveolar epithelial cells and to the alveolar macrophages. double label fluorescence employing fitc-coupled maclura pomifera lectin (mpa) and bbpa-avidin-texas red showed that bpa binding was confined to type i cells. in addition, a minor staining of the luminal border of the terminal bronchiolar epithelium was found. the sequence of tissue injury following x-radiation was examined in rats. bpa is ... | 1994 | 7894248 |
| galactose-containing glycoconjugates of the ciliary body and lens in capsular glaucoma: a lectin histochemical study. | this study was carried out in order to obtain information on galactose-containing glycoconjugates in the lens and ciliary body of human eyes with capsular glaucoma and thus on the etiopathogenesis of exfoliation syndrome. | 1994 | 8001826 |
| lambda foo: a lambda phage vector for the expression of foreign proteins. | this work describes a lambda phage expression system, lambda foo, that produces foreign proteins fused to the surface of the virus particle. the lambda foo vector has multiple cloning sites for the insertion of a foreign dna fragment and color selection for recombinants. foreign proteins are fused to the c terminus of a truncated phage tail protein, pv, by a peptide linker. conditional chain termination allows the assembly and fusion of multisubunit proteins. we have attached the complete escher ... | 1994 | 8058794 |
| bauhinia purpurea agglutinin (bpa) binding sites in human gastrointestinal tract. | the binding of biotinylated bpa to parraffin sections of 18 normal gastrointestinal tract mucosa, 5 nonneoplastic polyps (nnp), 12 adenomas, and 59 carcinomas was studied by using avidinbiotin peroxidase complex (abc) technique. in normal mucosa bpa appeared to bind both mucus and nonmucus glycoproteins but goblet cell mucus showed a decrease in binding and increase in binding of nonmucus glycoproteins as the cells lose their differentiation. bpa showed characteristic binding patterns in adenoma ... | 1994 | 8088897 |
| lectin histochemistry on the skin of hairless descendants of mexican hairless dogs. | the skin of hairless descendants of mexican hairless dogs was investigated lectin-histochemically. haired dogs were also used for comparative study. in the epidermis of infant pups, lectin staining revealed marked differences between hairless and haired dogs. griffonia simplicifolia-i (gs-i) bound moderately to the cytoplasm of the stratum basale of hairless dogs, while no binding to that of haired dogs was observed. bauhinia purpurea (bpa) showed positive staining for the stratum spinosum and b ... | 1994 | 8171420 |
| bauhinia purpurea lectin (bpa) binding spectra in hyperplastic human tonsil and in peripheral blood: immunohistochemical, immunoelectron microscopic, and flow cytometric analyses. | we studied the binding of bauhinia purpurea lectin (bpa) to human tonsils and peripheral blood mononuclear cells by immunohistochemical, immunoelectron microscopic, and flow cytometric techniques. the effect of several fixatives (acetone, ethanol, buffered and non-buffered formalin, b5, bodian 11, bouin's, carnoy's, zenker's, and glutaraldehyde) was also examined. bpa was reactive with germinal center lymphocytes, macrophages/histiocytes, follicular dendritic cells, squamous epithelial cells, an ... | 1993 | 8315273 |
| plasma membrane alterations of maturing goat (capra indicus) spermatozoa: lectin-binding and freeze-fracture study. | a qualitative and quantitative analysis of lectin-binding sites has been undertaken on spermatozoa recovered from different regions of the epididymis of the goat (capra indicus) using fluorescein isothiocyanate-linked lectins (bauhinia purpurea bpa, concanavalin a con a, dolichos biflorus dba, maclura pomifera mpa, arachis hypogaea or peanut agglutinin pna, glycine max or soyabean agglutinin sba, ulex europaeus uea, and triticum vulgaris or wheat-germ agglutinin wga), in conjunction with scannin ... | 1993 | 8443831 |
| carbohydrate and peptide antigens in macrophage populations derived from human bone marrow and milk: an immunomorphological and immunochemical analysis. | an immunomorphological and immunochemical study was performed to elucidate the pattern of carbohydrate antigens and their relationships to the cluster differentiation (cd) 68 epitopes on macrophages derived from human bone marrow and milk. core and backbone antigens recognized by lectins from bauhinia purpurea (bpa), helix pomatia (hpa), arachis hypogaea (pna), glycine max. (sba), griffonia simplicifolia (gsa-i-b4), lycopersicon esculentum (lea) and erythrina cristagalli (eca) were expressed by ... | 1995 | 8550384 |
| lectin recognition of host-like saccharide motifs in streptococcal cell wall polysaccharides. | viridans streptococci that participate in the microbial colonization of teeth have cell wall polysaccharides composed of linear phosphodiester-linked hexa- or heptasaccharide repeating units, each containing a host-like disaccharide motif, either gal beta 1-->3galnac or galnac beta 1-->3gal. whereas strains with galnac beta 1-->3gal-containing polysaccharides co-aggregated with streptococci that possess galnac-sensitive lectins, strains with either host-like motif co-aggregated with actinomyces ... | 1995 | 8608267 |
| interaction of a human blood group sd(a-) tamm-horsfall glycoprotein with applied lectins. | unlike the human blood group sd(a+) tamm-horsfall glycoprotein (thgp), the sd(a-) one lacks terminal galnacbeta1--> residues at the nonreducing ends. the binding properties of this glycoprotein and its asialo product with lectins were characterized by quantitative precipitin (qpa) and precipitin inhibition assays. among 20 lectins tested by qpa, both native and asialo sd(a-) thgp reacted best with abrus precatorius and ricinus communis and completely precipitated the lectin added. they also prec ... | 1996 | 8617360 |
| lectin histochemistry of pregnant rat uterine tissues. | glycoconjugate residues were examined in the rat uterus at days 10, 12 and 15 of pregnancy using 17 biotinylated lectins with specificities for a variety of carbohydrate moieties. a wide variety of glycoconjugate residues were detected in the cytoplasm of some antimesometrial and mesometrial decidual cells with lectins from canavalia ensiformis (con a), lens culinaris (lch) culinaris (lch), pisum sativum (psa), griffonia simplicifolia ii (gs-ii), triticum vulgaris (wga), griffonia simplicifolia ... | 1996 | 8655407 |
| binding properties of a blood group le(a+) active sialoglycoprotein, purified from human ovarian cyst, with applied lectins. | studies on the structures and binding properties of the glycoproteins, purified from human ovarian cyst fluids, will aid the understanding of the carbohydrate alterations occurring during the biosynthesis of blood group antigens and neoplasm formation. these glycoproteins can also serve as important biological materials to study blood group a, b, h, le(a), le(b), le(x), le(y), t and tn determinants, precursor type i and ii sequences and cold agglutinin i and i epitopes. in this study, the bindin ... | 1996 | 8672551 |
| alveolar type ii cell growth on a pulmonary endothelial extracellular matrix. | most of the alveolar epithelium overlies a fused basement membrane produced by epithelial and endothelial cells. to determine how this type of matrix influences type ii cell growth and function, we studied the effects of culturing isolated rat alveolar type ii cells on an extracellular matrix (ecm) freshly produced by pulmonary vascular endothelial cells grown 5 days in culture. type ii cells from the same rats were cultured on plastic or matrigel for comparison. a large increase in mitotic acti ... | 1996 | 8764228 |
| cellular heterogeneity in the membrana granulosa of developing rat follicles: assessment by flow cytometry and lectin binding. | the hormone-mediated maturation of ovarian follicles is apparently accompanied by position-specific differentiation of cells of the membrana granulosa. we have assessed the extent of this cellular heterogeneity by flow cytometry using a variety of fluorescein isothiocyanate-labeled lectins as probes. follicular development was stimulated in immature rats by treatment with either diethylstilbestrol (des) or equine cg (ecg). lectin binding to monodispersed rat granulosa cells was then analyzed by ... | 1996 | 8770935 |
| glycoconjugates and keratin 18 define subsets of taste cells. | sections of neonatal, normal adult and denervated adult rat tongue were examined with lectin histochemistry. attention was focused upon intragemmal cells (cells within the taste bud) and the surrounding perigemmal cells. informative staining patterns were observed with four of 12 lectins: ulex europaeus (uea-i), bauhinia purpurea (bpa), helix pomatia (hpa) and lotus tetragonolobus (lta) agglutinins. in normal adult tongues, bpa bound to those lingual epithelial cells lacking contact with the bas ... | 1995 | 8789401 |
| bauhinia purpurea and pisum sativum lectin binding in human breast. | the binding of biotinylated bauhinia purpurea (bpa) and pisum sativum (psa) lectins to paraffin-embedded tissue sections from 10 normal breast and 55 breast carcinoma has been investigated by applying avidin biotin peroxidase method (abc). bpa showed very low affinity for normal breast epithelium and the binding was confined to the luminal surface. eighty-seven percent carcinoma bound bpa, and the staining patterns varied depending on the histologic grade of tumors: luminal surface binding in gr ... | 1995 | 8819657 |
| changes in en(a-) human red blood cell membranes during in vivo ageing. | the human red blood cells with phenotype en(a-) were characterized by the lack of mn antigens. the red blood cells with phenotype en(a-) which were found in a japanese family were tested to clarify the changes in membrane surfaces of the red blood cells during in vivo ageing. the contents of sialic acid, glucose, mannose, galactose, fucose, n-acetylglucosamine and n-acetylgalactosamine of the red blood cell membranes obtained from the old red blood cells with phenotype en(a-) were significantly ... | 1996 | 8866734 |
| galactose-deficient iga1 in sera of iga nephropathy patients is present in complexes with igg. | iga1 proteins from sera of patients with iga nephropathy (igan) are galactosylated to a lesser degree than those from healthy controls. the increased reactivity of intact or de-sialylated serum iga1 with n-acetylgalactosamine (galnac)-specific lectins, helix aspersa (haa) and caragana arborescens (caa) and de-sialylated iga1 with helix pomatia (hpa) and bauhinia purpurea (bpa) indicated that the gal deficiency is in glycans located in the hinge region of iga1 molecules. de-sialylated iga from se ... | 1997 | 9264010 |
| differential binding properties of gal/galnac specific lectins available for characterization of glycoreceptors. | differentiating the binding properties of applied lectins should facilitate the selection of lectins for characterization of glycoreceptors on the cell surface. based on the binding specificities studied by inhibition assays of lectin-glycan interactions, over twenty gal and/or galnac specific lectins have been divided into eight groups according to their specificity for structural units (lectin determinants), which are the disaccharide as all or part of the determinants and of galnac alpha 1--> ... | 1997 | 9343930 |
| structural and antigenic types of cell wall polysaccharides from viridans group streptococci with receptors for oral actinomyces and streptococcal lectins. | lectin-mediated interactions between oral viridans group streptococci and actinomyces may play an important role in microbial colonization of the tooth surface. the presence of two host-like motifs, either galnac beta1-->3gal (gn) or gal beta1-->3galnac (g), in the cell wall polysaccharides of five streptococcal strains accounts for the lactose-sensitive coaggregations of these bacteria with actinomyces naeslundii. three streptococcal strains which have gn-containing polysaccharides also partici ... | 1997 | 9393793 |
| lectin binding sites on cd34+ human haematopoietic stem cells and lymphocytes from peripheral blood: an ultrastructural post-embedding study. | this study was performed to obtain a better insight into the glycosylation pattern of human cd34+ haematopoietic stem cells and lymphocytes from peripheral blood using an ultrastructural post-embedding technique. lectins applied were derived from canavalia ensiformis (con a), triticum vulgare (wga), lycopersicon esculentum (lea), limulus polyphemus (lpa), ulex europaeus-i (uea-i), bauhinia purpurea (bpa), glycine max (sba), helix pomatia (hpa), arachis hypogaea (pna) and erythrina cristagalli (e ... | 1997 | 9413743 |
| regulation of gelatinase b (mmp-9) in leukocytes by plant lectins. | the stimulatory or inhibitory effects of plant lectins on the production of gelatinase a (mmp-2) and gelatinase b (mmp-9) by mononuclear white blood cells was investigated by substrate zymography. leukocyte cultures from 24-h old buffy coats were spontaneously activated and produced high levels of gelatinase b. using such cultures the suppressing activity of the datura stramonium, viscum album, bauhinia purpurea, triticum aestivum and maackia amurensis lectins on gelatinase b induction were demo ... | 1998 | 9607327 |
| loss of immunoreactivity for rti40, a type i cell-specific protein in the alveolar epithelium of rat lungs with bleomycin-induced fibrosis. | after lung injury, the epithelial cells lining the alveolar surface in rat lung show an altered distribution of several membrane proteins. pulmonary fibrosis was induced by intratracheal administration of bleomycin into the lung of rats and the distribution of rti40, a recently detected alveolar epithelial type i cell antigen, was examined, as well as the relationship between rti40 and a type i cell-specific antigen recognized by the monoclonal antibody mep-1 and the type i cell-binding lectin b ... | 1998 | 9877498 |
| withania somnifera and bauhinia purpurea in the regulation of circulating thyroid hormone concentrations in female mice. | the effects of daily administration of withania somnifera root extract (1.4 g/kg body wt.) and bauhinia purpurea bark extract (2.5 mg/kg body wt.) for 20 days on thyroid function in female mice were investigated. while serum triiodothyronine (t3) and thyroxine (t4) concentrations were increased significantly by bauhinia, withania could enhance only serum t4 concentration. both the plant extracts showed an increase in hepatic glucose-6-phosphatase (g-6-pase) activity and antiperoxidative effects ... | 1999 | 10619390 |
| a chimeric lectin formed from bauhinia purpurea lectin and lens culinaris lectin recognizes a unique carbohydrate structure. | lectins are carbohydrate-binding proteins widely used in biochemical, immunochemical, and histochemical studies. bauhinia purpurea lectin (bpa) is a leguminous lectin with an affinity for galactose and lactose. nine amino acids, dtwpntews, corresponding to the amino acid sequence from aspartic acid-135 to serine-143 in the primary structure of bpa were replaced with the corresponding amino acid residues from the mannose-binding lens culinaris lectin (lca), and the chimeric lectin obtained was ex ... | 2000 | 10731675 |
| cyborg lectins: novel leguminous lectins with unique specificities. | bauhinia purpurea lectin (bpa) is one of the beta-galactose-binding leguminous lectins. leguminous lectins contain a long metal-binding loop, part of which determines their carbohydrate-binding specificities. random mutations were introduced into a portion of the cdna coding bpa that corresponds to the carbohydrate-binding loop of the lectin. an library of the mutant lectin expressed on the surface of lambda foo phages was screened by the panning method. several phage clones with an affinity for ... | 2000 | 10731676 |
| asaia bogorensis gen. nov., sp. nov., an unusual acetic acid bacterium in the alpha-proteobacteria. | eight gram-negative, aerobic, rod-shaped and peritrichously flagellated strains were isolated from flowers of the orchid tree (bauhinia purpurea) and of plumbago (plumbago auriculata), and from fermented glutinous rice, all collected in indonesia. the enrichment culture approach for acetic acid bacteria was employed, involving use of sorbitol medium at ph 3.5. all isolates grew well at ph 3.0 and 30 degrees c. they did not oxidize ethanol to acetic acid except for one strain that oxidized ethano ... | 2000 | 10758893 |
| characterisation of lectin binding patterns of mouse bronchiolar and rat alveolar epithelial cells in culture. | lung epithelial cell differentiation pathways remain unclear. this is due in part to the plasticity of these cells and the lack of markers which accurately reflect their differentiation status. the aim of this study was to determine if lectin binding properties are useful determinants of functional differentiation status in vitro. mouse clara cells were cultured for 5 days. during this time, no alteration in differentiation was evident by electron microscopy. no significant alteration in binding ... | 2000 | 10805383 |
| chemoenzymatic synthesis of glycopolypeptides carrying alpha-neu5ac-(2-->3)-beta-d-gal-(1-->3)-alpha-d-galnac, beta-d-gal-(1-->3)-alpha-d-galnac, and related compounds and analysis of their specific interactions with lectins. | glycopolypeptide (1) carrying the beta-d-gal-(1-->3)-alpha-d-galnac unit as a kind model of asialo-type mucin was synthesized through three steps: enzymatic synthesis of p-nitrophenyl disaccharide glycoside, reduction of the p-nitrophenyl group, and coupling of the amino group with the carboxyl group of poly(l-glutamic acid)s (pga). in a similar manner, glycopolypeptides (2-7) carrying beta-d-gal-(1-->3)-beta-d-galnac, beta-d-gal-(1-->3)-beta-d-glcnac, beta-d-gal-(1-->6)-alpha-d-galnac, beta-d-g ... | 2000 | 11097173 |
| leishmania (viannia) braziliensis metacyclic promastigotes purified using bauhinia purpurea lectin are complement resistant and highly infective for macrophages in vitro and hamsters in vivo. | in this study we characterised metacyclogenesis in axenic culture of leishmania (viannia) braziliensis, the causative agent of mucocutaneous leishmaniasis in the new world. metacyclogenesis of other species of leishmania has been shown by morphological changes as well as molecular modifications in the lipophosphoglycan, the major cell surface glycoconjugate of the promastigotes. in order to obtain metacyclic forms of l. braziliensis we tested a panel of different lectins. our results showed that ... | 2002 | 12350372 |
| recognition profile of bauhinia purpurea agglutinin (bpa). | bauhinia purpurea agglutinin (bpa) is a galbeta1-3galnac (t) specific leguminous lectin that has been widely used in multifarious cytochemical and immunological studies of cells and tissues under pathological or malignant conditions. despite these diverse applications, knowledge of its carbohydrate specificity was mainly limited to molecular or submolecular t disaccharides. thus, the requirement of high density polyvalent or multi-antennary carbohydrate structural units for bpa binding and an up ... | 2004 | 14741734 |
| flow cytometric analysis of lectin binding to in vitro-cultured perkinsus marinus surface carbohydrates. | parasite surface glycoconjugates are frequently involved in cellular recognition and colonization of the host. this study reports on the identification of perkinsus marinus surface carbohydrates by flow cytometric analyses of fluorescein isothiocyanate-conjugated lectin binding. lectin-binding specificity was confirmed by sugar inhibition and kolmogorov-smirnov statistics. clear, measurable fluorescence peaks were discriminated, and no parasite autofluorescence was observed. parasites (gtla-5 an ... | 2004 | 15270084 |
| evidence for the adhesive function of the exopolysaccharide of hyphomonas strain mhs-3 in its attachment to surfaces. | hyphomonas strain mhs-3 (mhs-3) is a marine procaryote with a biphasic life cycle and which has prosthecate stages that adhere to submerged substrata. we found that adherent forms produced an exopolysaccharide (eps) capsule that bound glycine max lectin, arachis hypogaea lectin, and bauhinia purpurea lectin (bpa), each having affinity for n-acetyl-d-galactosamine. it also bound the dye calcofluor. bpa and calcofluor were tested for the ability to hinder mhs-3 adhesion to glass surfaces; they red ... | 1995 | 16535028 |
| antineoplastic agents. 551. isolation and structures of bauhiniastatins 1-4 from bauhinia purpurea. | bioassay-guided (p388 lymphocytic leukemia cell line) separation of extracts prepared from the leaves, stems, and pods of bauhinia purpurea, and, in parallel, its roots, led to the isolation of four new dibenz[b,f]oxepins (2a, 3-5) named bauhiniastatins 1-4, as well as the known and related pacharin (1) as cancer cell growth inhibitors. the occurrence of oxepin derivatives in nature is quite rare. bauhiniastatins 1-4 were found to exhibit significant growth inhibition against a minipanel of huma ... | 2006 | 16562827 |
| antinociceptive, anti-inflammatory and antipyretic properties of the aqueous extract of bauhinia purpurea leaves in experimental animals. | the present study was carried out to determine the antinociceptive, anti-inflammatory and antipyretic activities of the aqueous extract of bauhinia purpurea leaves using animal models. | 2007 | 17917444 |
| purification and molecular cloning of a new galactose-specific lectin from bauhinia variegata seeds. | a new galactose-specific lectin was purified from seeds of a caesalpinoideae plant, bauhinia variegata, by affinity chromatography on lactose-agarose. protein extracts haemagglutinated rabbit and human erythrocytes (native and treated with proteolytic enzymes), showing preference for rabbit blood treated with papain and trypsin. among various carbohydrates tested, the lectin was best inhibited by d-galactose and its derivatives, especially lactose. sds-page showed that the lectin, named bvl, has ... | 2008 | 19005235 |
| amelioration of metformin-induced hypothyroidism by withania somnifera and bauhinia purpurea extracts in type 2 diabetic mice. | an investigation was carried out to reveal the possible ameliorative role of two plant extracts on an antidiabetic drug-induced hypothyroidism in type 2 diabetic animals. dexamethasone (1.0 mg/kg, i.m.) administration caused hyperglycemia with a parallel increase in renal lipid peroxidation (lpo), relative risk ratio (rr), and the concentrations of serum insulin; total cholesterol (tc); low-density lipoprotein cholesterol (ldl-c); very low-density lipoprotein cholesterol (vldl-c) and triglycerid ... | 2009 | 19170137 |
| survey of glycoantigens in cells from alpha1-3galactosyltransferase knockout pig using a lectin microarray. | glycoantigens represent major obstacles to successful xenotransplantation. even after the alpha1-3galactosyltransferase (galt) gene knockout (galt-ko) pigs were produced, non-gal antigens continue to be present. this study reports on lectin blot analyses for endothelial cells (ec) and fibroblasts from galt-ko pigs. | 2010 | 20149189 |
| screening of bauhinia purpurea linn. for analgesic and anti-inflammatory activities. | ethanol extract of the stem of bauhinia purpurea linn. was subjected to analgesic and anti-inflammatory activities in animal models. | 2009 | 20336222 |
| bauhinia variegata var. variegata trypsin inhibitor: from isolation to potential medicinal applications. | here we report for the first time of a new kunitz-type trypsin inhibitor (termed bvvti) from seeds of the camel's foot tree, bauhinia variegata var. variegata. bvvti shares the same reactive site residues (arg, ser) and exhibits a homology of n-terminal amino acid sequence to other bauhinia protease inhibitors. the trypsin inhibitory activity (k(i), 0.1 x 10(-9)m) of bvvti ranks the highest among them. besides anti-hiv-1 reverse transcriptase activity, bvvti could significantly inhibit the proli ... | 2010 | 20435016 |
| protective effect of bauhinia purpurea on gentamicin-induced nephrotoxicity in rats. | the present study was undertaken to evaluate the ethanol extract of leaves of bauhinia purpurea and unripe pods of bauhinia purpurea for its protective effects on gentamicin-induced nephrotoxicity in rats. nephrotoxicity was induced in wistar rats by intraperitoneal administration of gentamicin 100 mg/kg/d for eight days. effect of concurrent administration of ethanol extract of leaves of bauhinia purpurea and unripe pods of bauhinia purpurea at a dose of 300 mg/kg/d given by oral route was dete ... | 2009 | 20502576 |
| evaluation of wound healing potential of bauhinia purpurea leaf extracts in rats. | the present study was carried out to evaluate the effect of methanol and chloroform extracts of bauhinia purpurea on experimentally induced excision, incision, burn and dead space wound models in sprague dawley rats. formulations of methanol and chloroform extracts of bauhinia purpurea were prepared in carbopol and simple ointment base at concentrations of 2.5% and 5% and applied to the wounds. in the excision and burn wound models, animals treated with high doses of methanol and chloroform show ... | 2010 | 20582204 |
| interaction of proteinase inhibitors with phospholipid vesicles is modulated by ph. | rbbki and rbbci, plant recombinant inhibitors from bauhinia bauhinioides, and bputi from bauhinia purpurea seeds distinctly and specifically block proteolytic enzymes. the secondary structures of those inhibitors were compared and their interactions with phospholipid vesicles were evaluated by the release of calcein and by intrinsic fluorescence of tryptophan residues. the results show that rbbki, rbbci and bputi are able to interact with phospholipd vesicles and induce membrane permeabilization ... | 2010 | 20692285 |
| assessment of phytochemical content, polyphenolic composition, antioxidant and antibacterial activities of leguminosae medicinal plants in peninsular malaysia. | many medicinal plants from leguminosae family can be found easily in malaysia. these plants have been used as traditional medicines by local ethnic groups, where they are prepared as decoction, pastes for wound infections, and some have been eaten as salad. this paper focused on the assessment of antioxidant potential, antibacterial activity and classes of phytochemicals of nine plants from the leguminosae family. | 2011 | 21306653 |
| bacterial adherence in otitis media: determination of n-acetylgalactosamine (galnac) residues in the submucosal glands and surface epithelium of the normal and diseased eustachian tube. | acute otitis media (aom) is the most common childhood infection caused by bacteria. the pathogenesis of aom implicates initial adherence of a pathogen to the nasopharyngeal epithelium, which is followed by bacterial colonization of the middle ear cavity through the eustachian tube. n-acetylgalactosamine (galnac) is an important constituent of mucins and galnac containing sugar residues seem to be essential for initial adherence of respiratory bacteria to the surface of epithelial cells. | 2011 | 21440051 |
| in vivo antiulcer activity of the aqueous extract of bauhinia purpurea leaf. | ethnopharmacological relevance: bauhinia purpurea (fabaceae) is a medicinal plant traditionally used to treat various ailments, including ulcers. aim of the study: in order to establish pharmacological properties of the leaf of bauhinia purpurea, studies were performed on antiulcer activity of the plant's aqueous extract. materials and methods: the bauhinia purpurea aqueous extract (bpae) was prepared in the doses of 100, 500 and 1000mg/kg. antiulcer activity of bpae was evaluated by absolute et ... | 2011 | 21802502 |
| a potential human hepatocellular carcinoma inhibitor from bauhinia purpurea l. seeds: from purification to mechanism exploration. | a 20-kda kunitz-type trypsin-chymotrypsin inhibitor, bauhinia purpurea trypsin inhibitor (bplti), has been isolated from the seeds of b. purpurea l. by using liquid chromatography procedures that involved ion exchange chromatography on sp-sepharose and mono s and gel filtration on superdex 75. bplti demonstrated protease inhibitory activities of 7226 baee units/mg and 65 btee units/mg toward trypsin and α-chymotrypsin, respectively. bplti was relatively thermal (0-60°c) and ph (3-10) stable and ... | 2011 | 21969071 |
| embryonal carcinoma in the abdominal cavity of a male calf. | an embryonal carcinoma was diagnosed in the abdominal cavity of a 55-day-old male calf. macroscopically, a large volume of ascitic fluid was observed along with white to yellowish-white masses of various sizes densely located on the abdominal wall and the surface of abdominal organs. there was an absence of testes in the scrotum. histologically, the tumor cells were polygonal, and the tumor was mostly composed of sheets of densely packed solid patterns with occasional papillary and tubular struc ... | 2011 | 21908299 |
| assessment of anti-quorum sensing activity for some ornamental and medicinal plants native to egypt. | this study investigated the effects of some plant extracts on the bacterial communication system, expressed as quorum sensing (qs) activity. quorum sensing has a directly proportional effect on the amount of certain compounds, such as pigments, produced by the bacteria. alcohol extracts of 23 ornamental and medicinal plants were tested for anti-qs activity by the chromobacterium violaceum assay using the agar cup diffusion method. the screening revealed the anti-qs activity of six plants; namely ... | 2012 | 23641343 |