uptake and retention of heterologous dna applied to seeds of matthiola incana [proceedings]. 197779400
[the leafhopper neoaliturus haematoceps (mulsant & rey) is a vector of spiroplasma citri in the mediterranean].among the many leafhoppers which we have collected and identified in morocco, turkey, syria and france (corsica), only the species neoaliturus haematoceps was found to be infected with spiroplasma citri. in syria, this leafhopper was encountered on ornamental stock plants (matthiola incana l.) in the oronte valley, and on salsola kali l., a wild chenopodiacae of the semi-arid regions of the coastal area. in corsica, we found it on wild stock plants (matthiola sinuata of the oriental coast ...19863674783
two basic-helix-loop-helix genes (myc-146 and gl3) from arabidopsis can activate anthocyanin biosynthesis in a white-flowered matthiola incana mutant.basic helix-loop-helix (bhlh) proteins, similar to mammalian myc transcription factors, regulate the anthocyanin biosynthetic pathway in both monocots and dicots. two arabidopsis bhlh genes, glabra3 (gl3) and myc-146, encode proteins that are similar throughout the predicted amino acid sequence to r and delila, which regulate anthocyanin production in maize and snapdragon, respectively. northern blot analysis indicates that myc-146 is most highly expressed in flower buds and flowers. expression ...200312956536
identification and origin of xanthomonas campestris pv. campestris races and related pathovars.abstract one hundred sixty-four isolates of xanthomonas campestris pv. campestris and other x. campestris pathovars known to infect cruciferous hosts (x. campestris pvs. aberrans, raphani, armoraciae, and incanae) were inoculated onto a differential series of brassica spp. to determine both pathogenicity to brassicas and race. of these, 144 isolates were identified as x. campestris pv. campestris and grouped into six races, with races 1 (62%) and 4 (32%) being predominant. other races were rare. ...200118943594
cholesterol and triglyceride reduction in rats fed matthiola incana seed oil rich in (n-3) fatty acids.seeds of matthiola incana contain oil rich (55-65%) in (n-3) linolenic acid. selected lines were developed and evaluated for their agronomic and chemical parameters. extracted oil was fed for 6 weeks to rats, which were compared with rats fed a diet containing coconut oil or sunflower oil. cholesterol levels were significantly lowest in rats fed diets rich in m. incana oil (27% reduction), and triglycerides were significantly lower in rats receiving either m. incana or sunflower oil (36% reducti ...199910563945
structure of plant nuclear and ribosomal dna containing chromatin.digestion of plant chromatin from brassica pekinensis and matthiola incana with staphylococcus nuclease leads to a dna repeat of 175 plus or minus 8 and a core size of 140 base pairs. dnase i digestion results in multiples of 10 bases. ribosomal rnn genes were studied as a model system for active plant chromatin because of their great redundancy and their high transcriptional activity in growing and differentiating tissues. the actively transcribed genes were identified by nascent rna of ribosom ...1979514813
ribonucleic acid from the higher plant matthiola incana. molecular weight measurements and dna-rna hybridisation studies.the percentage of dna from the crucifer matthiola incana coding for different types of rna was measured by filter saturation hybridisation experiments using rna labelled in vivo. in addition, the melting curves of the various dna - rna hybrids formed and the buoyant densities of the dna sequences complementary to different types of rna were measured. 1. the rna preparations used were 25, 18, and 5 s rrna and 4 s rna, purified by gel electrophoresis, and poly(a)-containing rna purified by oligo-( ...1977322717
molecular characterization of flavanone 3 beta-hydroxylases. consensus sequence, comparison with related enzymes and the role of conserved histidine residues.a heterologous cdna probe from petunia hybrida was used to isolate flavanone-3 beta-hydroxylase-encoding cdna clones from carnation (dianthus caryophyllus), china aster (callistephus chinensis) and stock (matthiola incana). the deduced protein sequences together with the known sequences of the enzyme from p. hybrida, barley (hordeum vulgare) and snapdragon (antirrhinum majus) enabled the determination of a consensus sequence which revealed an overall 84% similarity (53% identity) of flavanone 3 ...19938223617
sequence organization and putative regulatory elements in the 5s rrna genes of two higher plants (vigna radiata and matthiola incana).the tandemly arranged and clustered highly repeated 5s rrna genes are investigated for two plants belonging to different higher plant families: matthiola incana (brassicaceae, dilleniidae, rosidae; 3600 5s rrna genes/n) shows a homogeneous repeat size of 510 bp, whereas vigna radiata (mung bean, former phaseolus aureus, fabaceae, rosidae; approx. 4300 5s rrna genes) has a repeat size of 215 bp. the mung-bean 5s rrna coding region starts 5' with agg and ends with cct; matthiola starts with ggg an ...19883371663
the structure of the major anthocyanin in arabidopsis thaliana.the major anthocyanin in the leaves and stems of arabidopsis thaliana has been isolated and shown to be cyanidin 3-o-[2-o(2-o-(sinapoyl)-beta-d-xylopyranosyl)-6-o-(4-o-(beta-d-glucopyranosyl)-p-coumaroyl-beta-d-glucopyranoside] 5-o-[6-o-(malonyl) beta-d-glucopyranoside]. this anthocyanin is a glucosylated version of one of the anthocyanins found in the flowers of the closely related matthiola incana.200211830144
herbicidal activity of sulforaphene from stock (matthiola incana).a herbicidal compound was isolated from extracts ofmatthiola incana and identified as sulforaphene (4-methylsulfinyl-3-butenyl isothiocyanate). the ed50 of this compound against velvetleaf seedlings was approximately 2×10(-4) m. glucoraphenin, the glucosinolate that is the natural precursor of sulforaphene, was less phytotoxic, with an ed50 of near 6×10(-3)m.199324248575
effect of 8-hydroxyquinoline on the uptake of uridine and incorporation into rna.8-hydroxyquinoline has been previously used as an inhibitor in studies on porphyrin metabolism, where it is thought to act by chelating iron. it is shown that this compound also rapidly inhibits uridine uptake of seedlings or cotyledons of the crucifer matthiola incana rna synthesis is also affected but the inhibition is not as severe as reported for fission yeast.197724419694
incorporation of t4 phage dna into a specific dna fraction from the higher plant matthiola incana.isolated t4 phage dna (sigma=1.694 g/ml) is applied to seedlings of the crucifer matthiola incana (dna density sigma=1.698 g/ml). the phage dna can partly be reextracted from the plants in a specific dna fraction, which is predominantly characterized by its unusual high density (high density complex=hdc; sigma=1.724 g/ml). dna:dna hybridization studies show that phage specific dna sequences are preserved in the hdc. results of brdurd labeling of the plant dna before and during incubation with t4 ...1976136126
two wd-repeat genes from cotton are functional homologues of the arabidopsis thaliana transparent testa glabra1 (ttg1) gene.cotton fibres are single, highly elongated cells derived from the outer epidermis of ovules, and are developmentally similar to the trichomes of arabidopsis thaliana. to identify genes involved in the molecular control of cotton fibre initiation, we isolated four putative homologues of the arabidopsis trichome-associated gene transparent testa glabra1 (ttg1). all four wd-repeat genes are derived from the ancestral d diploid genome of tetraploid cotton and are expressed in many tissues throughout ...200515821869
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