| anti-hyperglycemic effect of some edible plants. | the anti-hyperglycemic effect of 12 edible plants was studied on 27 healthy rabbits, submitted weekly to subcutaneous glucose tolerance tests after gastric administration of water, tolbutamide or a traditional preparation of the plant. tolbutamide, cucurbita ficifolia, phaseolus vulgaris, opuntia streptacantha, spinacea oleracea, cucumis sativus and cuminum cyminum decrease significantly the area under the glucose tolerance curve and the hyperglycemic peak. brassica oleracea var. botrytis, alliu ... | 1995 | 8569244 |
| a structure-specific endonuclease from cauliflower (brassica oleracea var. botrytis) inflorescence. | a protein with structure-specific endonuclease activity has been purified to near homogeneity from cauliflower ( brassica oleracea var. botrytis) inflorescence through five successive column chromatographies. the protein is a single polypeptide with a molecular mass of 40 kda. using three different branched dna structures (flap, pseudo-y and stem-loop) we found that the enzyme, a cauliflower structure-specific endonuclease, cleaved the single-stranded tail in the 5'-flap and 5'-pseudo-y structur ... | 1997 | 9396804 |
| potential of a novel antibiotic, 2-methylheptyl isonicotinate, as a biocontrol agent against fusarial wilt of crucifers. | screening for newer bioactive compounds from microbial metabolites resulted in the isolation of a novel antibiotic from the culture filtrate, streptomyces sp 201, of a soil. the bioactive compound, with antifungal and antibacterial activity, was identified as 2-methylheptyl isonicotinate. the antifungal activity of live culture, culture broth and the isolated bioactive compound showed marked inhibition against dominant soil-borne phytopathogens such as fusarium oxysporum schlect, f moniliforme s ... | 2002 | 11975177 |
| s-methyl methanethiosulfonate, bio-antimutagen in homogenates of cruciferae and liliaceae vegetables. | the isolation of a new type of bio-antimutagen, s-methyl methanethiosulfonate (mmts), from cauliflower (brassica oleracea var. botrytis) and the distribution and formation of mmts in cruciferae and liliaceae vegetables are described. for the separation and purification, cauliflower curds were homogenized, extracted with acetone, and then purified by organic solvent extraction and by various processes of chromatographic separation. the chemical structure of an active principle was identified as m ... | 1996 | 8987591 |
| chloroplast dna topoisomerase i from cauliflower. | an atp-independent dna topoisomerase has been isolated from chloroplasts of cauliflower leaves (brassica oleracea var. botrytis) through deae-cellulose, af-blue toyopearl, and hydroxyapatite column chromatography. the sedimentation coefficient and stokes radius of this enzyme are 3.6s and 3.6 nm, respectively, and the molecular weight of native enzyme is estimated to be 54,000. this enzyme changes the linking number in steps of one. the enzyme activity is stimulated by mgcl2, and this enzyme sho ... | 1991 | 1849883 |
| plant pyruvate dehydrogenase complex purification, characterization and regulation by metabolites and phosphorylation. | the pyruvate dehydrogenase complex was purified from mitochondria of cauliflower, brassica oleracea var. botrytis floral buds to a specific activity of 5.4 mumol of nadh/min per mg of protein. the pyruvate dehydrogenase complex required coash, nad+, thiamine pyrophosphate and mg2+ for the oxidative decarboxylation of pyruvate. the kinetic analysis of the complex gave a series of parallel lines for all substrates. product interaction patterns showed that nadh is competitive with nad+; acetyl-coa ... | 1977 | 922017 |
| comparative studies on polyguanylate polymerase and polyadenylate polymerase activities in the dna-dependent rna polymerase i fraction from cauliflower. | the properties of poly(g) polymerase and poly(a) polymerase activities in the dna-dependent rna polymerase nucleosidetriphosphate: rna nucleotidyltransferase ec 2.7.7.6 i fraction from cauliflower (brassica oleracea var. botrytis) were comparatively investigated. the ph optimum, the effect of ionic strength, the effect of substrate concentration on the rate of synthesis, the effect of divalent metal ion concentration, and the time course of synthesis at different temperatures were all different ... | 1976 | 7554 |
| characterization of two dna polymerases from cauliflower inflorescence. | two distinct dna polymerases, a and b, isolated from a rapidly growing apical tissue of cauliflower (brassica oleracea var. botrytis) inflorescence have been characterized, and compared with dna polymerases, alpha and beta, from mouse myeloma. polymerase-a bears a strong resemblance to polymerase-alpha from mammalian cells in all properties examined. the character of polymerase-b is also quite similar to polymerase-beta of mammalian cells in chromatographic elution properties, template-primer ut ... | 1980 | 7390985 |
| the c-s lyases of higher plants : isolation and properties of homogeneous cystine lyase from broccoli (brassica oleracea var botrytis) buds. | cystine lyase degrades l-cystine by a beta-elimination to form cysteine persulfide, pyruvate, and ammonia. this enzyme is common in brassica sp. and has been purified to homogeneity from extracts of broccoli (brassica oleracea var botrytis) buds. two isozymes were separated on deae-fractogel columns and the first peak, cystine lyase i further purified to homogeneity. the purified enzyme had a narrow range of substrate specificity with l-cystine and s-alkyl-l-cysteine sulfoxides being the primary ... | 1986 | 16664688 |
| an evaluation of 4-s-methyl-2-keto-butyric acid as an intermediate in the biosynthesis of ethylene. | stimulation of ethylene production by cauliflower (brassica oleracea var. botrytis l.) tissue in buffer solution containing 4-s-methyl-2-keto-butyric acid is not due to activation of the natural in vivo system. increased ethylene production derives from an extra-cellular ethylene-forming system, catalyzed by peroxidase and other factors, which leak from the cauliflower tissue and cause the degradation of 4-s-methyl-2-keto-butyric acid. this exogenous ethylene-forming system is similar to the eth ... | 1971 | 16657663 |
| serine transhydroxymethylase of cauliflower (brassica oleracea var. botrytis l.): partial purification and properties. | serine transhydroxymethylase (ec 2.1.2.1) has been purified 46-fold from cauliflower (brassica oleracea var. botrytis l.). the enzyme was completely dependent on the presence of tetrahydrofolic acid for the conversion of serine to glycine. the addition of pyridoxal phosphate gave a large increase in the reaction rate. a double ph optimum was observed with maxima at 7.5 and 9.5. the enzyme is specific for l-serine. the d-isomer is neither a substrate nor an inhibitor. the michaelis constants for ... | 1967 | 16656717 |
| molecular basis of the cauliflower phenotype in arabidopsis. | genetic studies demonstrate that two arabidopsis genes, cauliflower and apetala1, encode partially redundant activities involved in the formation of floral meristems, the first step in the development of flowers. isolation of the cauliflower gene from arabidopsis reveals that it is closely related in sequence to apetala1. like apetala1, cauliflower is expressed in young flower primordia and encodes a mads-domain, indicating that it may function as a transcription factor. analysis of the cultivat ... | 1995 | 7824951 |
| [cloning of acc oxidase gene and inhibition of endogenous gene expression with rnai in cauliflower]. | a fragment of 1202 bp of the candidate aco gene was amplified from the cauliflower (brassica oleracea var. botrytis) genome using the degenerated primers which were designed according to the consensus sequence of aco amino acids among various plant species. the result of blast showed the sequence presented a very high match with the aco genes from other plants; the homologue was from 83% to 99%. three exons and two introns were identified in this sequence. the spliced length of mrna was 756 nt a ... | 2005 | 16078747 |
| the phytoalexins from cauliflower, caulilexins a, b and c: isolation, structure determination, syntheses and antifungal activity. | our continuous search for phytoalexins from crucifers led us to examine phytoalexin production in florets of cauliflower (brassica oleracea var. botrytis) under abiotic (uv light) elicitation. four known (isalexin, s-(-)-spirobrassinin, 1-methoxybrassitin, brassicanal c) and three new (caulilexins a-c) phytoalexins were isolated. the syntheses and antifungal activity of caulilexins a-c against the economically important pathogenic fungi leptosphaeria maculans, rhizoctonia solani and sclerotinia ... | 2006 | 16806330 |
| genotypic effects on glucosinolates and sensory properties of broccoli and cauliflower. | the objective of this study was to investigate the influence that the species, form or cultivar exerts on the glucosinolate spectrum for glucosinolates and free sugars in selected vegetable species of the brassica genus (brassica oleracea var. italica plenck, brassica rapa var. alboglabra and brassica oleracea var. botrytis l.). the results showed significant differences amongst the cultivar groups for the glucosinolate proportions as well as the contents of health-promoting and flavour-influenc ... | 2004 | 15053347 |
| [regulation to curd morphogenesis and genetics of exogenous bocal gene in cauliflower]. | cauliflower (brassica oleracea var. botrytis l.) and cabbage (b. oleracea var capitata l.) are two varieties of species b. oleracea. cauliflower bobcal gene losses the original function due to its terminated mutation while cabbage bocal gene has the complete function. we obtained cauliflower plants transgenic for bocal gene using arobacterium-mediated transformation. genetic analysis of t2 generation indicated that all cauliflower plants transgenic for bocal failed to form curd and produced the ... | 2003 | 14574987 |
| persistence of carbaryl residues in and on unprocessed and processed cauliflower curds. | carbaryl was applied three times at 15 days interval to cauliflower (brassica oleracea var. botrytis l.), and its persistence was determined spectrophotometrically at 0, 1, 3, 6, 10, and 15 days after the first and last treatments. in unprocessed cauliflower the prescribed tolerance level for carbaryl residues of 5 ppm was only reached on the sixth day, in processed cauliflower on the third day after application. pretreatment with washing + boiling/cooking on the other hand led to a considerable ... | 1982 | 6807280 |
| brassica oleracea pollen, a new source of occupational allergens. | vegetable pollen is a rare source of occupational allergens. occupational allergy has only been described in the case of paprika pollen and tomato pollen. we describe a new source of occupational pollen allergy. | 2006 | 16512813 |
| [identification of pcr markers associated with cytoplasmic male sterility in brassica oleracea var botrytis]. | the homology-based candidate gene method was used to identified the specific pcr markers linked to cytoplasmic male sterility (cms) in cauliflower( brassica oleracea var botrytis.). searching the dna and protein data-base of ncbi , correlative genes or open reading frames were identified . analysis of biosoft, based on the conservative regions ,five primers were designed . among them, only primer p9/p10 produced a 313- bp specific fragment. identified by individual plant testing , analysis of rt ... | 2005 | 15843352 |
| antifungal activity of brassica oleracea var. botrytis fresh aqueous juice. | the antifungal activity of fresh, aqueous brassica oleracea var. botrytis juice against candida albicans and other pathogenic fungi was investigated. the juice was found to be effective both in inhibiting the growth of blastoconidia and reducing the appearance of c. albicans germ tubes. furthermore, the juice inhibited the growth of some pathogenic, filamentous fungi. | 2003 | 12837360 |
| purification of a plant nucleotide pyrophosphatase as a protein that interferes with nitrate reductase and glutamine synthetase assays. | an activity that inhibited both glutamine synthetase (gs) and nitrate reductase (nr) was highly purified from cauliflower (brassica oleracea var. botrytis) extracts. the final preparation contained an acyl-coa oxidase and a second protein of the plant nucleotide pyrophosphatase family. this preparation hydrolysed nadh, atp and fad to generate amp and was inhibited by fluoride, cu2+, zn2+ and ni2+. the purified fraction had no effect on the activity of nr when reduced methylviologen was used as e ... | 2003 | 12631294 |
| n-linked glycosylation of native and recombinant cauliflower xyloglucan endotransglycosylase 16a. | the gene encoding a xet (xyloglucan endotransglycosylase) from cauliflower ( brassica oleracea var. botrytis ) florets has been cloned and sequenced. sequence analysis indicated a high degree of similarity to other xet enzymes belonging to glycosyl hydrolase family 16 (gh16). in addition to the conserved gh16 catalytic sequence motif eidfe, there exists one potential n-linked glycosylation site, which is also highly conserved in xet enzymes from this family. purification of the corresponding pro ... | 2003 | 12826015 |
| genetic transformation of cauliflower (brassica oleracea var. botrytis) by direct dna uptake into mesophyll protoplasts. | mesophyll protoplasts of brassica oleracea var. botrytis were successfully transformed using polyethylene glycol (peg). the success of plant transformation depended on both gene transfer and plant regeneration. parameters, such as peg and vector concentrations and heat shock conditions were tested in experiments on transient expression of the beta-glucuronidase (ec 3.2.1.31) gene and the most suitable conditions for dna uptake were determined. two antibiotic resistance marker genes for neomycin ... | 2002 | 12060266 |
| highly informative nature of inter simple sequence repeat (issr) sequences amplified using tri- and tetra-nucleotide primers from dna of cauliflower (brassica oleracea var. botrytis l.). | inter simple sequence repeat (issr) sequences as molecular markers can lead to the detection of polymorphism and also be a new approach to the study of ssr distribution and frequency. in this study, issr amplification with nonanchored primer was performed in closely related cauliflower lines. fourty-four different amplified fragments were sequenced. sequences of pcr products are delimited by the expected motifs and number of repeats, which validates the issr nonanchored primer amplification tech ... | 2002 | 12416621 |
| the cauliflower or gene encodes a dnaj cysteine-rich domain-containing protein that mediates high levels of beta-carotene accumulation. | despite recent progress in our understanding of carotenogenesis in plants, the mechanisms that govern overall carotenoid accumulation remain largely unknown. the orange (or) gene mutation in cauliflower (brassica oleracea var botrytis) confers the accumulation of high levels of beta-carotene in various tissues normally devoid of carotenoids. using positional cloning, we isolated the gene representing or and verified it by functional complementation in wild-type cauliflower. or encodes a plastid- ... | 2006 | 17172359 |
| modification of enzyme sulfhydryl groups suppresses uv-induced mutagenesis depending on the nucleotide excision repair system in escherichia coli b/r wp2. | s-methyl methanethiosulfonate (mmts), which was isolated from cauliflower (brassica oleracea var. botrytis) homogenate as a potent bio-antimutagen, has been used as an enzyme-sulfhydryl (sh) temporary blocking agent in modification studies of enzyme activities. we examined whether 23 kinds of mmts-related compounds have a suppressing effect on uv mutagenesis in escherichia coli b/r wp2. disulfide derivatives of diphenyl, 2.2'-dipyridine and 4.4'-dipyridine, and n-ethyl maleimide (nem), which tem ... | 1998 | 9539980 |
| [construction of genetic linkage map and localization of nbs-lrr like resistance gene analogues in cauliflower (brassica oleracea var. botrytis)]. | nucleotide binding site (nbs) profiling, a new method was used to map resistance gene analogues (rgas) in cauliflower (brassica oleracea var. botrytis). this method allows amplification and the mapping of genetic markers anchored in the conserved nbs encoding domain of plant disease resistance genes. aflp was also performed to construct the cauliflower intervarietal genetic map. the aim of constructing genetic map was to identify potential molecular markers linked to important agronomic traits t ... | 2007 | 17650494 |
| meristem identity gene expression during curd proliferation and flower initiation in brassica oleracea. | the regulation of reproductive development in cauliflower (brassica oleracea var. botrytis dc) and broccoli (b. oleracea l. var. italica plenck) is unusual in that most enlargement occurs while development is arrested at a distinct stage. cauliflower and broccoli curds are composed of inflorescence meristems and flower buds, respectively. to determine whether this arrest is maintained by altered expression of the genes that specify these steps in arabidopsis, the expression of each copy of their ... | 2008 | 18332227 |
| [isolation and identification of specific sequences correlated to cytoplasmic male sterility and fertile maintenance in cauliflower (brassica oleracea var. botrytis)]. | analysis of issr (inter-simple sequence repeat) and ddrt-pcr (differential display reverse transcriptase polymerase chain reaction) was performed between cytoplasmic male sterility cauliflower ogura-a and its corresponding maintainer line ogura-b. totally, 306 detectable bands were obtained by issr using thirty oligonucleotide primers. commonly, six to twelve bands were produced per primer. among all these primers only the amplification of primer issr3 was polymorphic, an 1100 bp specific band w ... | 2008 | 18464586 |
| uptake of the pharmaceutical fluoxetine hydrochloride from growth medium by brassicaceae. | since the european union banned disposal of sewage sludge (ss) at sea in 1998 the application rate of ss to land has risen significantly. land application is thus possibly an important transport route for ss-associated organic chemicals, including pharmaceuticals, to soils and perhaps also to plants. the potential for the selective serotonin re-uptake inhibitor, fluoxetine hcl, to undergo uptake into brassicaceae tissues was therefore investigated in a tissue culture study under laboratory condi ... | 2008 | 18723196 |
| a novel gene mutation that confers abnormal patterns of beta-carotene accumulation in cauliflower (brassica oleracea var. botrytis). | the or gene of cauliflower (brassica oleracea var. botrytis) causes many tissues of the plant to accumulate carotenoids and turn orange, which is suggestive of a perturbation of the normal regulation of carotenogenesis. a series of experiments to explore the cellular basis of the carotenoid accumulation induced by the or gene was completed. the or gene causes obvious carotenoid accumulation in weakly or unpigmented tissues such as the curd, pith, leaf bases and shoot meristems, and cryptically i ... | 2001 | 11359610 |
| development of resistance against blackleg disease in brassica oleracea var. botrytis through in silico methods. | blackleg caused by leptosphaeria maculans is a very important disease worldwide and causes large yield loss of significant vegetable crops of brassicaceae family. absence of resistant 'b' genome in vegetable brassica oleracea proposed these species susceptible to blackleg disease. we present a specific piece of molecular modelling work, combining in silico docking methods, energy minimization calculations and in silico cloning, to develop a rational peptide as a candidate that functions as a res ... | 2010 | 20619351 |
| diverse accumulation of several dehydrin-like proteins in cauliflower (brassica oleracea var. botrytis), arabidopsis thaliana and yellow lupin (lupinus luteus) mitochondria under cold and heat stress. | dehydrins represent hydrophilic proteins acting mainly during cell dehydration and stress response. dehydrins are generally thermostable; however, the so-called dehydrin-like (dehydrin-related) proteins show variable thermolability. both groups immunoreact with antibodies directed against the k-segment of dehydrins. plant mitochondrial dehydrin-like proteins are poorly characterized. the purpose of this study was to extend previous reports on plant dehydrins by comparing the level of immunopreci ... | 2010 | 20718974 |
| purification and characterization of a 100 kda dna polymerase from cauliflower inflorescence. | a dna polymerase from cauliflower (brassica oleracea var. botrytis) inflorescence has been purified to near homogeneity through five successive column chromatographies, and temporally designated cauliflower polymerase 1. cauliflower polymerase 1 is a monopolypeptide with a molecular mass of 100 kda. the enzyme efficiently uses synthetic dna homopolymers and moderately activated dna and a synthetic rna homopolymer as template-primers. the enzyme is strongly sensitive to dideoxythymidine triphosph ... | 1998 | 9601087 |
| the cauliflower orange gene enhances petiole elongation by suppressing expression of eukaryotic release factor 1. | • the cauliflower (brassica oleracea var. botrytis) orange (or) gene affects plant growth and development in addition to conferring β-carotene accumulation. this study was undertaken to investigate the molecular basis for the effects of the or gene mutation in on plant growth. • the or protein was found to interact with cauliflower and arabidopsis eukaryotic release factor 1-2 (erf1-2), a member of the erf1 family, by yeast two-hybrid analysis and by bimolecular fluorescence complementation (bif ... | 2010 | 21175633 |
| dissipation kinetics of spinosad on cauliflower (brassica oleracea var. botrytis. l.) under subtropical conditions of punjab, india. | residues of spinosad were estimated in cauliflower curds using high performance liquid chromatography (hplc) and confirmed by high performance thin layer chromatography (hptlc). following three application of spinosad (success 2.5 sc) at 15 and 30 g a.i. ha−1, the average initial deposits of spinosad were observed to be 0.57 and 1.34 mg kg−1, respectively. these residues dissipated below the limit of quantification (loq) of 0.02 mg kg−1 after 10 days at both the dosages. the half-life values (t ... | 2009 | 19672547 |
| production and characterization of interspecific somatic hybrids between brassica oleracea var. botrytis and b. nigra and their progenies for the selection of advanced pre-breeding materials. | somatic hybridization is a potential method for gene transfer from wild relatives to cultivated crops that can overcome sexual incompatibilities of two distantly related species. in this study, interspecific asymmetric somatic hybrids of brassica oleracea var. botrytis (cauliflower) and brassica nigra (black mustard) were obtained by protoplast fusion and their backcrossed (bc(3)) and selfed (s(3)) offspring were analyzed. cytological analysis showed that the b. nigra chromosomes were successive ... | 2011 | 21603996 |
| influence of various host plants on the consumption and utilization of food by pieris brassicae (linn.). | pieris brassicae (linn.) is a destructive cosmopolitan pest of cruciferous crops. it is present wherever its host plants occur, and it is considered to be one of the most widely distributed of all the lepidoptera. we investigated the affect of various host plants on the food consumption and utilization by p. brassicae. we quantified consumption of food, larval duration, pupal duration and weight on cabbage (brassica oleracea var. capitata), cauliflower (brassica oleracea var. botrytis), radish ( ... | 2011 | 22030306 |
| shoot differentiation, regeneration of cauliflower and analysis of somaclonal variation by rapd. | our major goal in this study was to establish and characterize an efficient callus induction and shoot regeneration protocol for cauliflower (brassica oleracea var. botrytis). the effects of induction medium, growth regulator combination, organic component, agno(3), genotype and explants type on shoot differentiation on the cauliflower regeneration process were taken in account and hereby evaluated. the optimal media for shoot differentiation and rooting were modified ms medium (ms with pg-96 or ... | 2006 | 17362340 |
| expression of brassica oleracea ftsz1-1 and mind alters chloroplast division in nicotiana tabacum generating macro- and mini-chloroplasts. | ftsz1-1 and mind plastid division-related genes were identified and cloned from brassica oleracea var. botrytis. transgenic tobacco plants expressing boftsz1-1 or bomind exhibited cells with either fewer but abnormally large chloroplasts or more but smaller chloroplasts relative to wild-type tobacco plants. an abnormal chloroplast phenotype in guard cells was found in bomind transgenic tobacco plants but not in boftsz1-1 transgenic tobacco plants. transgenic tobacco plants bearing the macro-chlo ... | 2011 | 22193339 |
| reaction of cauliflower genotypes to black rot of crucifers. | this study aimed to evaluate six cauliflower genotypes regarding their resistance to black rot and their production performance. to do so, it was conducted two field experiments in ipameri, goiás, brazil, in 2012 and 2013. it was used a randomized block design, with four replications (total of 24 plots). each plot consisted of three planting lines 2.5 m long (six plants/line), spaced 1.0 m apart, for a total area of 7.5 m(2). evaluations of black rot severity were performed at 45 days after tran ... | 2015 | 26060437 |
| characterization of free nitrogen fixing bacteria of the genus azotobacter in organic vegetable-grown colombian soils. | with the purpose of isolating and characterizing free nitrogen fixing bacteria (fnfb) of the genus azotobacter, soil samples were collected randomly from different vegetable organic cultures with neutral ph in different zones of boyacá-colombia. isolations were done in selective free nitrogen ashby-sucrose agar obtaining a recovery of 40%. twenty four isolates were evaluated for colony and cellular morphology, pigment production and metabolic activities. molecular characterization was carried ou ... | 2011 | 24031700 |
| developing novel bacterial based bioformulation having pgpr properties for enhanced production of agricultural crops. | plant growth promoting rhizobacteria (pgpr) are beneficial rhizobacteria which enhance plant growth as well as the productivity by a variety of mechanisms pgpr were isolated from the rhizosphere region of som plants (machilus bombycina king) maintained at the central muga eri research and training institute, lahdoigarh, jorhat. a bacterial based bioformulation was prepared and sprayed over the experimental crops including tomato (solanum lycopersicum), cauliflower (brassica oleracea var botrytis ... | 2015 | 25675713 |
| accumulation of heavy metals in the vegetables grown in wastewater irrigated areas of dehradun, india with reference to human health risk. | the present study on accumulation of heavy metals in the vegetables viz. beta vulgaris, phaseolus vulgaris, spinacea oleracea, and brassica oleracea var. botrytis grown in the wastewater-irrigated soil near the bindal river, dehradun, has shown the maximum accumulation of metals for pb (196.91 ± 8.13 mg/kg), cu (36.75 ± 6.19 mg/kg), zn (305.54 ± 14.30 mg/kg), ni (125.48 ± 5.97 mg/kg), cd (29.58 ± 4.26 mg/kg), and cr (93.06 ± 3.25 mg/kg) in agricultural soil irrigated with wastewater. the enrichm ... | 2015 | 26092239 |
| quantitative trait loci controlling leaf appearance and curd initiation of cauliflower in relation to temperature. | qtl regions on chromosomes c06 and c09 are involved in temperature dependent time to curd induction in cauliflower. temperature is the main environmental factor influencing curding time of cauliflower (brassica oleracea var. botrytis). temperatures above 20-22 °c inhibit development towards curding even in many summer cultivars. to identify quantitative trait loci (qtl) controlling curding time and its related traits in a wide range of different temperature regimes from 12 to 27 °c, a doubled ha ... | 2016 | 26993486 |
| genetic variation of temperature-regulated curd induction in cauliflower: elucidation of floral transition by genome-wide association mapping and gene expression analysis. | cauliflower (brassica oleracea var. botrytis) is a vernalization-responsive crop. high ambient temperatures delay harvest time. the elucidation of the genetic regulation of floral transition is highly interesting for a precise harvest scheduling and to ensure stable market supply. this study aims at genetic dissection of temperature-dependent curd induction in cauliflower by genome-wide association studies and gene expression analysis. to assess temperature-dependent curd induction, two greenhou ... | 2015 | 26442034 |
| [identification and analysis of asymmetric somatic hybrids between b. oleracea and b. nigra]. | asymmetric interspecific somatic hybrids between brassica oleracea var. botrytis and b. nigra were produced by peg-induced fusion of protoplasts radiated by different doses of uv. b. nigra, genotype st.461, has resistance to black rot, black leg and clubroot diseases which are popular in cabbage production. the regenerated plants were analyzed by several means including morphology observation, relative dna content measurement by flow cytometry (fcm), chromosome counting, dna molecular marker. mo ... | 2008 | 18959000 |
| Identification and expression analysis of CBF/DREB1 and COR15 genes in mutants of Brassica oleracea var. botrytis with enhanced proline production and frost resistance. | Frost resistant mutants of Brassica oleracea var. botrytis were investigated for the presence of CBF/DREB1 and COR15a gene products and induced frost resistance. Total RNA of clones was isolated after 3 h, 6 h, 24 h and 14 d acclimation at 4 °C and proteins and free proline were isolated after 14 d acclimation. cDNA was produced using RT-PCR and the first CBF gene in B. oleracea detected and did quantify. Through SDS-PAGE and Western blotting, the COR15a protein was detected for the first time i ... | 2011 | 22000056 |
| biology and life table parameters of brevicoryne brassicae (hemiptera: aphididae) on cauliflower cultivars. | in this article, the biology and fertility life table parameters of the cabbage aphid, brevicoryne brassicae (l.) (hemiptera: aphididae), were studied on cauliflower leaves, brassica oleracea var. botrytis (brassicales: brassicaceae), of the cultivars smilla, snow mystique, white cloud, buris, galiblanka, snow crown, sg, and tokita. this study was conducted under controlled conditions: 25 ± 2°c, 65 ± 5% relative humidity (rh), and 16:8 (l:d) h photoperiods. statistical analysis showed that there ... | 2014 | 25527591 |
| the role of boflc2 in cauliflower (brassica oleracea var. botrytis l.) reproductive development. | in agricultural species that are sexually propagated or whose marketable organ is a reproductive structure, management of the flowering process is critical. inflorescence development in cauliflower is particularly complex, presenting unique challenges for those seeking to predict and manage flowering time. in this study, an integrated physiological and molecular approach was used to clarify the environmental control of cauliflower reproductive development at the molecular level. a functional all ... | 2015 | 25355864 |
| engineering a sensitive visual-tracking reporter system for real-time monitoring phosphorus deficiency in tobacco. | plant phosphorus (p) diagnosis is widely used for monitoring p status and guiding p fertilizer application in field conditions. the common methods for predicting plant response to p are time- and labour-consuming chemical measurements of the extractable soil p and plant p concentrations. in this study, we successfully generated a visual reporter system in tobacco (nicotiana tabacum l.) to monitor plant p status by expressing of a purple gene (pr) isolated from cauliflower (brassica oleracea var ... | 2014 | 25187932 |
| combined alkaline hydrolysis and ultrasound-assisted extraction for the release of nonextractable phenolics from cauliflower (brassica oleracea var. botrytis) waste. | cauliflower waste contains high amounts phenolic compounds, but conventional solvent extraction misses high amounts of nonextractable phenolics (nep), which may contribute more to the valorization of these waste streams. in this study, the nep content and composition of cauliflower waste were investigated. the ability of alkaline hydrolysis, sonication, and their combination to release nep was assessed. alkaline hydrolysis with sonication was found to extract the highest nep content (7.3 ± 0.17 ... | 2014 | 24665894 |
| in-depth analysis of internal control genes for quantitative real-time pcr in brassica oleracea var. botrytis. | quantitative reverse-transcription pcr (qrt-pcr) is a versatile technique for the analysis of gene expression. the selection of stable reference genes is essential for the application of this technique. cauliflower (brassica oleracea l. var. botrytis) is a commonly consumed vegetable that is rich in vitamin, calcium, and iron. thus far, to our knowledge, there have been no reports on the validation of suitable reference genes for the data normalization of qrt-pcr in cauliflower. in the present s ... | 2016 | 27525844 |
| genetic and epigenetic alterations of brassica nigra introgression lines from somatic hybridization: a resource for cauliflower improvement. | broad phenotypic variations were obtained previously in derivatives from the asymmetric somatic hybridization of cauliflower "korso" (brassica oleracea var. botrytis, 2n = 18, cc genome) and black mustard "g1/1" (brassica nigra, 2n = 16, bb genome). however, the mechanisms underlying these variations were unknown. in this study, 28 putative introgression lines (ils) were pre-selected according to a series of morphological (leaf shape and color, plant height and branching, curd features, and flow ... | 2016 | 27625659 |
| influence of sulforaphane metabolites on activities of human drug-metabolizing cytochrome p450 and determination of sulforaphane in human liver cells. | the influence of metabolites of sulforaphane, natural compounds present in broccoli (brassica oleracea var. botrytis italica) and in other cruciferous vegetables, on drug-metabolizing cytochrome p450 (cyp) enzymes in human liver microsomes and possible entry of sulforaphane into human hepatic cells were investigated. metabolites studied are compounds derived from sulforaphane by the mercapturic acid pathway (conjugation with glutathione and by following reactions), namely sulforaphane glutathion ... | 2016 | 27779894 |
| introgression of black rot resistance from brassica carinata to cauliflower (brassica oleracea botrytis group) through embryo rescue. | black rot caused by xanthomonas campestris pv. campestris (xcc) is a very important disease of cauliflower (brassica oleracea botrytis group) resulting into 10-50% yield losses every year. since there is a dearth of availability of resistance to black rot disease in b. oleracea (c genome), therefore exploration of a and b genomes was inevitable as they have been reported to be potential reservoirs of gene(s) for resistance to black rot. to utilize these sources, interspecific hybrid and backcros ... | 2017 | 28769959 |
| an extensive hairy root production precedes shoot regeneration in protoplast-derived calli of cauliflower (brassica oleracea var. botrytis). | protoplasts isolated from mesophyll tissues of cauliflower (brassica oleraceavar. botrytis) were induced to divide in culture, with 2% of them producing calli. upon transfer to a regeneration medium containing a low auxin/cytokinin balance (0.02mg/l 1-naphthaleneacetic and 2mg/l 6-benzylaminopurine), they displayed an extensive production of hairy roots before the regeneration of shoots. negative effects of root differentiation on the subsequent caulogenesis by such calli were not observed, sinc ... | 1992 | 24201437 |
| genotype, plant, bud size and media factors affecting anther culture of cauliflowers (brassica oleracea var. botrytis). | eleven f1 hybrid cultivars of cauliflower, representing a range of maturity types, were examined for their responsiveness to anther culture. embryos were produced from each of the cultivars tested, and the mean embryo yield varied from 82.2 embryos per 100 anthers cultured for cv dova to 0.6 embryos for cv serrano. variation between genotypes and between plants within a genotype was significant, both in terms of embryo yield and percentage responsive anthers. autumn and winter maturing cauliflow ... | 1990 | 24226116 |
| promotive role of 5-aminolevulinic acid on chromium-induced morphological, photosynthetic, and oxidative changes in cauliflower (brassica oleracea botrytis l.). | chromium (cr) is among the most toxic pollutants in the environment that adversely affect the living organisms and physiological processes in different plants. the present study investigated the effect of 15 mg l(-1) of 5-aminolevulinic acid (ala) on morpho-physiological attributes of cauliflower (brassica oleracea botrytis l.) under different cr concentrations (0, 10, 100, and 200 μm) in the growth medium. the results showed that cr stress decreased the growth, biomass, photosynthetic, and gas ... | 2017 | 28214935 |
| characterization of s-nitrosoglutathione reductase from brassica and lactuca spp. and its modulation during plant development. | cellular homeostasis of s-nitrosoglutathione (gsno), a major cache of nitric oxide bioactivity in plants, is controlled by the nadh-dependent s-nitrosoglutathione reductase (gsnor) belonging to the family of class iii alcohol dehydrogenases (ec 1.1.1.1). gsnor is a key regulator of s-nitrosothiol metabolism and is involved in plant responses to abiotic and biotic stresses. this study was focused on gsnor from two important crop plants, cauliflower (brassica oleracea var. botrytis, bogsnor) and l ... | 2016 | 27940345 |
| evaluation of various soaking agents as a novel tool for pesticide residues mitigation from cauliflower (brassica oleracea var. botrytis). | the increasing use of pesticides for boosting the yield of agricultural crops also impart toxic residues which ultimately extend to numerous physiological disorders upon consumption. the present study was designed as an effort to assess the reduction potential of various chemical solutions and to minimize the pesticide residues in cauliflower (brassica oleracea var. botrytis). the samples were soaked in various solutions along with tap water to mitigate pesticide residues. afterwards, the extrac ... | 2016 | 27784925 |
| effect of biologically synthesized copper oxide nanoparticles on metabolism and antioxidant activity to the crop plants solanum lycopersicum and brassica oleracea var. botrytis. | study on the ecological effect of metal oxide nanomaterials (nms) has quickly amplified over the precedent years because it is assumed that these nms will sooner or later be released into the environment. the present study deals with biologically oriented process for the green synthesis of copper oxide nanoparticles (cuo nps) by using morus alba leaf extract as reducing agent. powder x-ray diffraction (xrd) and transmission electron microscope (tem) analysis revealed the monoclinic phase and 20- ... | 2017 | 28962841 |
| biomethanation macrodynamics of vegetable residues pretreated by low-frequency microwave irradiation. | the effects of microwave irradiation on the digestibility and biogas production of cauliflower (brassica oleracea var. botrytis) and cabbage (brassica oleracea var. capitata) leaves were investigated using biochemical methane potential (bmp) assays. cow dung was utilised as inoculum. different microwave powers (87.5, 175 and 350w) were applied in a first set of runs for 15min. the second set consisted of 20, 25 and 30min irradiation at 350w. based on anova analysis (α=0.05), biogas production wa ... | 2018 | 28602662 |
| biogenesis of mitochondria in cauliflower (brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, organellar translation and ultrastructure. | the biogenesis of the cauliflower curd mitochondrial proteome was investigated under cold, heat and the recovery. for the first time, two dimensional fluorescence difference gel electrophoresis was used to study the plant mitochondrial complexome in heat and heat recovery. particularly, changes in the complex i and complex iii subunits and import proteins, and the partial disintegration of matrix complexes were observed. the presence of unassembled subunits of atp synthase was accompanied by imp ... | 2016 | 25617518 |
| in vitro propagation of cauliflower using curd microexplants. | cauliflower (brassica oleracea var. botrytis) with its distinctive pre-inflorescence or curd is a remarkable member of the brassica cabbage group. during curd development, intense and repetitive branching leads to a spectacular increase in size and the accumulation of millions of meristems at its surface. although destined to produce flowers, most of these meristems are capable of regenerating vegetative shoots in vitro, making curd fragments an excellent material for the micropropagation of cau ... | 2013 | 23179711 |
| effect of packaging on storage life and quality of cauliflower stored at low temperature. | medium sized cauliflower (brassica oleracea var. botrytis) curds after removing outer leaves, sorting and without washing were packed in different packaging films and stored at 0 ± 1°c, 90-95% rh. changes in curd colour, texture, physiological loss in weight, spoilage and sensory quality were evaluated at weekly interval upto 28 days. cauliflower curds individual packed in high density polyethylene bags (20 μm) with perforation (6 holes/bag) can be stored up to 21 days at 0 ± 1°c and 90-95% rh w ... | 2010 | 23572616 |
| effect of hydrothermal treatment on the antioxidant properties of broccoli (brassica oleracea var. botrytis italica) florets. | in the extracts of fresh raw and frozen broccoli, caffeic, ferulic, sinapinic acids and kaempferol were identified. boiling reduced the amounts of caffeic acid and kaempferol in both samples. the concentration of polyphenols was 2.69mg/g fresh mass and 0.96mg/fresh mass in fresh raw broccoli and frozen raw broccoli, respectively. boiling significantly decreased the amounts of phenolic compounds in fresh broccoli (1.58mg/g of fresh mass). in the case of frozen broccoli, boiling increased the conc ... | 2008 | 26003363 |
| the control of nad specific malic enzyme from cauliflower bud mitochondria by metabolites. | nad malic enzyme (ec. 1.1.1.39) has been purified from cauliflower (brassica oleracea. var. botrytis) bud mitochondria. the enzyme exhibits complex regulatory properties being activated by a variety of metabolites including glycolytic intermediates, coa, sulphate and krebs cycle acids-the tricarboxylic acids with the exception of citrate being more effective than dicarboxylic acids. fructose diphosphate which is a positive effector of the enzyme increases the affinity of the enzyme for l-malate. ... | 1975 | 24430215 |
| isolation and characterization of dna topoisomerase ii from cauliflower inflorescences. | type ii dna topoisomerase has been isolated from inflorescences of cauliflower (brassica oleracea var. botrytis) through a sequence of polyethylene glycol fractionation, ammonium sulfate precipitation, and column chromatography on cm-sephadex, hydroxyapatite and phosphocellulose. the molecular weight of the native enzyme, based on sedimentation coefficient (9s) and gel filtration analysis (stokes radius, 60 å), was estimated to be 223 000. this enzyme was able to catalyze fully the relaxation of ... | 1986 | 24307272 |
| impact of synergistic association of zno-nanorods and symbiotic fungus piriformospora indica dsm 11827 on brassica oleracea var. botrytis (broccoli). | in the present work, novel nanotool called 'nano-embedded fungus' formed by impact of synergistic association of zno-nanorods and fungus piriformospora indica dsm 11827, for growth of brassica oleracea var. botrytis (broccoli) is reported. zno-nanorods were synthesized by mechanical assisted thermal decomposition process and characterized by scanning electron microscopy (sem) for morphology, x-ray diffraction for structural studies and uv-vis absorption spectroscopy for band gap determination. n ... | 2017 | 29089926 |
| genomic prediction and association mapping of curd-related traits in genebank accessions of cauliflower. | genetic resources are an important source of genetic variation for plant breeding. genome-wide association studies (gwas) and genomic prediction greatly facilitate the analysis and utilization of useful genetic diversity for improving complex phenotypic traits in crop plants. we explored the potential of gwas and genomic prediction for improving curd-related traits in cauliflower (brassica oleracea var. botrytis) by combining 174 randomly selected cauliflower genebank accessions from two differe ... | 2017 | 29255118 |
| the purple cauliflower arises from activation of a myb transcription factor. | anthocyanins are responsible for the color of many flowers, fruits, and vegetables. an interesting and unique purple (pr) gene mutation in cauliflower (brassica oleracea var botrytis) confers an abnormal pattern of anthocyanin accumulation, giving the striking mutant phenotype of intense purple color in curds and a few other tissues. to unravel the nature of the pr mutation in cauliflower, we isolated the pr gene via a combination of candidate gene analysis and fine mapping. pr encoded a r2r3 my ... | 2010 | 20855520 |
| phenological and phytochemical changes correlate with differential interactions of verticillium dahliae with broccoli and cauliflower. | cauliflower (brassica oleracea var. botrytis subvar. cauliflora) is susceptible to wilt caused by verticillium dahliae but broccoli (b. oleracea var. italica subvar. cyamosa) is not. infection of broccoli and cauliflower by a green fluorescent protein-expressing isolate of v. dahliae was examined using epifluorescence and confocal laser-scanning microscopy to follow infection and colonization in relation to plant phenology. plant glucosinolate, phenolic, and lignin contents were also assayed at ... | 2011 | 21219133 |
| interactions between cauliflower and rhizoctonia anastomosis groups with different levels of aggressiveness. | the soil borne fungus rhizoctonia is one of the most important plant pathogenic fungi, with a wide host range and worldwide distribution. in cauliflower (brassica oleracea var. botrytis), several anastomosis groups (ags) including both multinucleate r. solani and binucleate rhizoctonia species have been identified showing different levels of aggressiveness. the infection and colonization process of rhizoctonia during pathogenic interactions is well described. in contrast, insights into processes ... | 2009 | 19622152 |