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effects of composting on phytotoxicity of spent pig-manure sawdust litter.the phytotoxicity of spent pig-manure sawdust litter (spent litter) was evaluated during further composting. aqueous extracts of the spent litter were prepared by shaking the sample with water (1:10 w/v), and the toxicity of these extracts was determined on relative seed germination, relative root elongation and germination index (gi, a factor of relative seed germination and relative root elongation). the sensitivity of six plant species, namely brassica parachinensis (chinese cabbage), brassic ...199615093523
antimicrobial effect of extracts of cruciferous vegetables.the cruciferous vegetables cauliflower, broccoli, cabbage, chinese radish, chinese kale, and chinese kitam were used in this study to prepare water-soluble and methanol-water extracts. crude protein extracts were also obtained by diethylaminoethyl (deae) anion exchange chromatography. water-soluble polysaccharides were prepared by ethanol precipitation followed by ultrafiltration. the antimicrobial effects of all these extracts were evaluated against gram-positive bacteria, gram-negative bacteri ...200415696789
decreased toxicity to terrestrial plants associated with a mixture of methyl tert-butyl ether and its metabolite tert-butyl alcohol.the influence of the main fuel oxygenate methyl tert-butyl ether (mtbe) and its key metabolite, tert-butyl alcohol (tba), on the growth of a plant seedling was studied separately and in combination. the test plants were mung bean (phaseolus radiatus), cucumber (cucumis sativus), wheat (triticum aestivum), sorghum (sorghum bicolor), kale (brassica alboglabra), chinese cabbage (brassica campestris), and sweet corn (zea mays). the growth of all the plants was adversely affected by tba and mtbe. the ...200717702346
a novel and exploitable antifungal peptide from kale (brassica alboglabra) seeds.the aim of this study was to purify and characterize antifungal peptides from kale seeds in view of the paucity of information on antifungal peptides from the family brassicaceae, and to compare its characteristics with those of published brassica antifungal peptides. a 5907-da antifungal peptide was isolated from kale seeds. the isolation procedure comprised affinity chromatography on affi-gel blue gel, ion exchange chromatography on sp-sepharose and mono s, and gel filtration on superdex pepti ...200818597893
isolation and characterization of juncin, an antifungal protein from seeds of japanese takana (brassica juncea var. integrifolia).an 18.9 kda antifungal protein designated juncin was isolated from seeds of the japanese takana (brassica juncea var. integrifolia). the purification protocol employed comprised anion-exchange chromatography on q-sepharose, affinity chromatography on affi-gel blue gel, cation exchange chromatography on sp-sepharose, and gel filtration on superdex 75. juncin was adsorbed on affi-gel blue gel and sp-sepharose but unadsorbed on q-sepharose. the protein exhibited antifungal activity toward the phyto ...200919354248
development of a new biofertilizer with a high capacity for n2 fixation, phosphate and potassium solubilization and auxin production.biofertilizers that possess a high capacity for n(2) fixation (azotobacter tropicalis), and consist of phosphate solubilizing bacteria (burkhoderia unamae), and potassium solubilizing bacteria (bacillus subtilis) and produce auxin (kjb9/2 strain), have a high potential for growth and yield enhancement of corn and vegetables (chinese kale). for vegetables, the addition of biofertilizer alone enhanced growth 4 times. moreover, an enhancement of growth by 7 times was observed due to the addition of ...201020460702
in vitro antitumor activity of broccolini seeds extracts.broccolini (brassica oleracea italica +ù alboglabra) is a hybrid of broccoli and kai-lan, chinese broccoli. to date, no report on antitumor activity of broccolini (not broccoli) is available. in this study, we evaluated the antiproliferative effects of broccolini seeds extract (bse) on human lung and ovarian cancer cells. it was found that bse induces a549 and ovcar-3 cells apoptosis in a dose-dependent manner by using mtt assay. the ic(50) values of bse in a549 and ovcar-3 cells were estimated ...201121695707
responses of growing japanese quails that received selenium from selenium enriched kale sprout (brassica oleracea var. alboglabra l.).the objectives of this study were to determine the effect of selenium (se) from se-enriched kale sprout (brassica oleracea var. alboglabra l.) on the performance and se concentrations in tissues of growing japanese quails. two hundred quails were divided into five treatments. each treatment consisted of four replicates and each replicate contained ten quails in a completely randomize design. the experiment was conducted for 5-áweeks. the treatments were t1, control diet; t2, control diet plus 0. ...201121681467
efficacy of selenium from hydroponically produced selenium-enriched kale sprout (brassica oleracea var. alboglabra l.) in broilers.an experiment was conducted to determine the efficacy of se from hydroponically produced se-enriched kale sprout (hpseks) on performance, carcass characteristics, tissue se concentration, and physiological responses of broilers in comparison to that of se from se-enriched yeast and sodium selenite. three hundred and sixty male broilers, 10 days of age, were assigned to 6 groups, 4 replicates of 15 broilers each, according to the completely randomized design. the dietary treatments were the follo ...201525586621
productivity and selenium concentrations in egg and tissue of laying quails fed selenium from hydroponically produced selenium-enriched kale sprout (brassica oleracea var. alboglabra l.).this study aimed to determine the effectiveness of se from hydroponically produced se-enriched kale sprout (hpseks) on productive performance, egg quality, and se concentrations in egg and tissue of laying quails. two-hundred quails, 63 days of age, were divided into four groups. each group consisted of five replicates and each replicate had ten birds, according to a completely randomized design. the experiment lasted for 6 weeks. the dietary treatments were t1 (control diet), t2 (control diet p ...201324057425
determination of vitamin c, b-carotene and riboflavin contents in five green vegetables organically and conventionally grown.as consumer interest in organically grown vegetables is increasing in malaysia, there is a need to answer whether the vegetables are more nutritious than those conventionally grown. this study investigates commercially available vegetables grown organically and conventionally, purchased from retailers to analyse β-carotene, vitamin c and riboflavin contents. five types of green vegetables were selected, namely chinese mustard (sawi) (brassica juncea), chinese kale (kai-lan) (brassica alboglabra) ...200322692530
comparative physical mapping of segments of the genome of brassica oleracea var. alboglabra that are homoeologous to sequenced regions of chromosomes 4 and 5 of arabidopsis thaliana.due to their relatedness to arabidopsis thaliana (arabidopsis), the cultivated brassica species represent the first group of crops with which to evaluate comparative genomics approaches to understanding biological processes and manipulating traits. we have constructed a high-quality binary bac library (jbo) from genomic dna of brassica oleracea var. alboglabra, in order to underpin such investigations. using the arabidopsis genome sequence and clones from the jbo library, we have analysed aspect ...200010929117
glutathione s-transferase slgste1 in spodoptera litura may be associated with feeding adaptation of host plants.spodoptera litura is polyphagous pest insect and feeds on plants of more than 90 families. in this study the role of glutathione s-transferase epilson 1 (slgste1) in s. litura in detoxification was examined. this gene was up-regulated in the midgut of s. litura at the transcriptional and protein levels when the insect fed on brassica juncea or diet containing phytochemicals such as indole-3-carbinol and allyl-isothiocyanate that are metabolic products of sinigrin and glucobrassicin in b. juncea. ...201626631599
efficiency of plant induced volatiles in attracting encarsia formosa and serangium japonicum, two dominant natural enemies of whitefly bemisia tabaci in china.whitefly bemisia tabaci is a globally distributed and most destructive pest to agriculture. owing to increasing chemical resistance, a long-lasting strategy to manage this pest must involve biological control. herbivore-induced plant volatiles (hipvs) usually play a profoundly important role in the foraging behaviour of natural enemies. here, the effects of hipvs from chinese broccoli on the foraging behaviour of two dominant natural enemy species of b. tabaci in china, encarsia formosa and sera ...201424488542
safety assessment and detection method of genetically modified chinese kale (brassica oleracea cv. alboglabra ).sporamins are tuberous storage proteins and account for 80% of soluble protein in sweet potato tubers with trypsin-inhibitory activity. the expression of sporamin protein in transgenic chinese kale (line boa 3-1) conferred insecticidal activity toward corn earworm [ helicoverpa armigera (hubner)] in a previous report. in this study, we present a preliminary safety assessment of transgenic chinese kale boa 3-1. bioinformatic and simulated gastric fluid (sgf) analyses were performed to evaluate th ...200919216530
comparative transcriptome analyses reveal a special glucosinolate metabolism mechanism in brassica alboglabra sprouts.brassica sprouts contain abundant phytochemicals, especially glucosinolates (gss). various methods have been used to enhance gs content in sprouts. however, the molecular basis of gs metabolism in sprouts remains an open question. here we employed rna-seq analysis to compare the transcriptomes of high-gs (jl-08) and low-gs (jl-09) brassica alboglabra sprouts. paired-end illumina rna-seq reads were generated and mapped to the brassica oleracea reference genome. the differentially expressed genes ...201627757119
overexpression of atedt1/hdg11 in chinese kale (brassica oleracea var. alboglabra) enhances drought and osmotic stress tolerance.plants are constantly challenged by environmental stresses, including drought and high salinity. improvement of drought and osmotic stress tolerance without yield decrease has been a great challenge in crop improvement. the arabidopsis enhanced drought tolerance1/homeodomain glabrous11 (atedt1/hdg11), a protein of the class iv hd-zip family, has been demonstrated to significantly improve drought tolerance in arabidopsis, rice, and pepper. here, we report that atedt1/hdg11 confers drought and osm ...201627625663
low temperature affects stem cell maintenance in brassica oleracea seedlings.most of the above ground tissues in higher plants originate from stem cells located in the shoot apical meristem (sam). several plant species can suffer from spontaneous stem cell arrest resulting in lack of further shoot development. in brassica oleracea this sam arrest is known as blindness and occurs in an unpredictable manner leading to considerable economic losses for plant raisers and farmers. detailed analyses of seedlings showed that stem cell arrest is triggered by low temperatures duri ...201627375654
interaction of light quality and fertility on biomass, shoot pigmentation and xanthophyll cycle flux in chinese kale.nutritionally important carotenoids in 21-day-old brassica microgreens increase following short and long-term exposure to narrow-band wavelengths from light-emitting diodes (led). the present study aimed to measure the impact of: (1) fluorescent/incandescent light and different percentages of blue/red led light and (2) different levels of nutrient fertility on biomass and pigment concentrations in 30-day-old 'green lance' chinese kale (brassica oleracea var. alboglabra). kale plants were exposed ...201727220007
development of a novel allele-specific rfo marker and creation of ogura cms fertility-restored interspecific hybrids in brassica oleracea.a novel allele-specific rfo marker was developed and proved to be effective for mas of rfo gene in b. oleracea background and six ogu-cms fertility-restored interspecific hybrids were created for the first time. ogura cytoplasmic male sterility (ogu-cms) has been extensively used for brassica oleracea hybrid production. however, because of maternal inheritance, all the hybrids produced by cms lines are male sterile and cannot be self-pollinated, which prohibits germplasm maintenance and innovati ...201627206841
interspecific hybridization, polyploidization, and backcross of brassica oleracea var. alboglabra with b. rapa var. purpurea morphologically recapitulate the evolution of brassica vegetables.brassica oleracea and b. rapa are two important vegetable crops. both are composed of dozens of subspecies encompassing hundreds of varieties and cultivars. synthetic b. napus with these two plants has been used extensively as a research model for the investigation of allopolyploid evolution. however, the mechanism underlying the explosive evolution of hundreds of varieties of b. oleracea and b. rapa within a short period is poorly understood. in the present study, interspecific hybridization be ...201626727246
molecular cloning, expression pattern and genotypic effects on glucoraphanin biosynthetic related genes in chinese kale (brassica oleracea var. alboglabra bailey).glucoraphanin is a plant secondary metabolite that is involved in plant defense and imparts health-promoting properties to cruciferous vegetables. in this study, three genes involved in glucoraphanin metabolism, branched-chain aminotransferase 4 (bcat4), methylthioalkylmalate synthase 1 (mam1) and dihomomethionine n-hydroxylase (cyp79f1), were cloned from chinese kale (brassica oleracea var. alboglabra bailey). sequence homology and phylogenetic analysis identified these genes and confirmed the ...201526569208
food safety in thailand 2: pesticide residues found in chinese kale (brassica oleracea), a commonly consumed vegetable in asian countries.there is increasing public concern over human health risks associated with extensive use of pesticides in agriculture. regulation of pesticide maximum residue limits (mrls) in food commodities is established in many developed countries. for thailand, this regulation exists in law but is not fully enforced. therefore, pesticide residues in vegetables and fruits have not been well monitored. this study investigated the pesticide residues in chinese kale, a commonly eaten vegetable among asians. th ...201526093223
variation of glucosinolates and quinone reductase activity among different varieties of chinese kale and improvement of glucoraphanin by metabolic engineering.the variation of glucosinolates and quinone reductase (qr) activity in fourteen varieties of chinese kale (brassica oleracea var. alboglabra bailey) was investigated in the present study. results showed that gluconapin (gna), instead of glucoraphanin (gra), was the most predominant glucosinolate in all varieties, and qr activity was remarkably positively correlated with the glucoraphanin level. aop2, a tandem 2-oxoglutarate-dependent dioxygenase, catalyzes the conversion of glucoraphanin to gluc ...201525172716
insecticide residues in head lettuce, cabbage, chinese cabbage, and broccoli grown in fields.the residues of four insecticides belonging to different families were studied on head lettuce (lactuca sativa l. var. capitata l.), cabbage (brassica oleracea linn. var. capitata dc.), chinese cabbage (brassica pekinensis skeels), and broccoli (brassica oleracea var. italica) after pesticide application. to reduce application variability, a tank mix of acetamiprid 20% sp, chlorpyrifos 22.5% ec, deltamethrin 2.4% sc, and methomyl 40% sp was applied at recommended and double doses. initial deposi ...201424684565
evaluation of genetic diversity in chinese kale (brassica oleracea l. var. alboglabra bailey) by using rapid amplified polymorphic dna and sequence-related amplified polymorphism markers.chinese kale is an original chinese vegetable of the cruciferae family. to select suitable parents for hybrid breeding, we thoroughly analyzed the genetic diversity of chinese kale. random amplified polymorphic dna (rapd) and sequence-related amplified polymorphism (srap) molecular markers were used to evaluate the genetic diversity across 21 chinese kale accessions from avrdc and guangzhou in china. a total of 104 bands were detected by 11 rapd primers, of which 66 (63.5%) were polymorphic, and ...201424615113
exposure factors for wastewater-irrigated asian vegetables and a probabilistic rotavirus disease burden model for their consumption.many farmers in water-scarce regions of developing countries use wastewater to irrigate vegetables and other agricultural crops, a practice that may expand with climate change. there are a number of health risks associated with wastewater irrigation for human food crops, particularly with surface irrigation techniques common in the developing world. the world health organization (who) recommends using quantitative microbial risk assessment (qmra) to determine if the irrigation scheme meets healt ...201424576153
spent mushroom waste as a media replacement for peat moss in kai-lan (brassica oleracea var. alboglabra) production.peat moss (pm) is the most widely used growing substrate for the pot culture. due to diminishing availability and increasing price of pm, researchers are looking for viable alternatives for peat as a growth media component for potted plants. a pot study was conducted with a view to investigate the possibility of using spent mushroom waste (smw) for kai-lan (brassica oleracea var. alboglabra) production replacing peat moss (pm) in growth media. the treatments evaluated were 100% pm (control), 100 ...201324106452
testing insecticidal activity of novel chemically synthesized sirna against plutella xylostella under laboratory and field conditions.over the last 60 years, synthetic chemical pesticides have served as a main tactic in the field of crop protection, but their availability is now declining as a result of the development of insect resistance. therefore, alternative pest management agents are needed. however, the demonstration of rnai gene silencing in insects and its successful usage in disrupting the expression of vital genes opened a door to the development of a variety of novel, environmentally sound approaches for insect pes ...201323667556
rapid estimation of glucosinolate thermal degradation rate constants in leaves of chinese kale and broccoli (brassica oleracea) in two seasons.kinetic modeling was used as a tool to quantitatively estimate glucosinolate thermal degradation rate constants. literature shows that thermal degradation rates differ in different vegetables. well-characterized plant material, leaves of broccoli and chinese kale plants grown in two seasons, was used in the study. it was shown that a first-order reaction is appropriate to model glucosinolate degradation independent from the season. no difference in degradation rate constants of structurally iden ...201222816876
inhibitory effects of broccolini leaf flavonoids on human cancer cells.broccolini (brassica oleracea italica × alboglabra) is a hybrid between broccoli and gai lan, also known as chinese broccoli and chinese kale. the aim of this study was to assess the antitumor activity of broccolini leaf flavonoids (blf). cell growth inhibition was evaluated using a standard colorimetric mtt assay, cellular morphology was observed using phase contrast microscopy and flow cytometry was introduced to further investigate cells apoptosis effect. the results showed that blf possess a ...201222532078
volatile components of ethanolic extract from broccolini leaves.broccolini (brassica oleracea italica × alboglabra) is a hybrid of broccoli and kai-lan, chinese broccoli. to date, no study has been reported on the chemical composition of the volatile fractions of this raw material. in this study, the volatile constituents from the ethanolic extract of broccolini leaves were analysed by gas chromatography-mass spectrometry (gc-ms). sixteen compounds were identified. the major components include 5-phenyl-undecane (11%), n-hexadecanoic acid (9.34%), octadecanoi ...201221859369
fine mapping of a male sterility gene ms-cd1 in brassica oleracea.a dominant male sterility (dgms) line 79-399-3, developed from a spontaneous mutation in brassica oleracea var. capitata, has been widely used in production of hybrid cultivars in china. in this line, male sterility is controlled by a dominant gene ms-cd1. in the present study, fine mapping of ms-cd1 was conducted by screening a segregating population ms79-07 with 2,028 individuals developed by four times backcrossing using a male sterile brassica oleracea var. italica line harboring ms-cd1 as d ...201121538103
extraction and identification of isothiocyanates from broccolini seeds.broccolini (brassica oleracea italica x alboglabra) is a cross between broccoli and kai-lan (chinese broccoli), which contains abundant glucosinolates. the intact glucosinolates are believed to be inactive, while their hydrolysis products, such as isothiocyanates (itcs), are found to have bacteriocidal and anticarcinogenic activities. so far, no report is available about generation of itcs during the process of glucosinolate hydrolysis in broccolini. in this study, the hydrolysis of broccolini s ...201121366048
molecular cytogenetic analysis of brassica rapa-brassica oleracea var. alboglabra monosomic addition lines.interspecific alien chromosome addition lines can be very useful for gene mapping and studying chromosome homoeology between closely related species. in this study we demonstrate a simple but robust manner of identifying individual c-genome chromosomes (c5, c8 and c9) in the a-genome background through the simultaneous use of 5s and 25s ribosomal probes on mitotic and meiotic chromosomes of three different brassica rapa-b. oleracea var. alboglabra monosomic addition lines. sequential silver stai ...200515756535
chloroplast and nuclear dna studies in a few members of the brassica oleracea l. group using pcr-rflp and issr-pcr markers: a population genetic analysis.a population genetic analysis of chloroplast and nuclear dna was performed covering nine wild populations of brassica oleracea. three members of the n = 9 group, all close to b. oleracea, brassica alboglabra bailey, brassica bourgeaui (webb) o. kuntze and brassica montana pourret, were also studied to better understand their relationship with b. oleracea. chloroplast dna was analysed using the pcr-rflp (polymerase chain reaction - restriction fragment length polymorphism) method. the issr-pcr (i ...200312671762
nitrogen leaching from soil treated with sludge.the amount of sludge generated from urban centers is increasing more and more, so wastewater treatment plants are being constructed. recycling of sludge by application to agricultural land can alleviate the disposal pressure, and, at the same time, utilize the plant nutrients in the waste. organic nitrogen in sludge is mineralized to inorganic forms such as nitrate and ammonium that can be taken up by plants. the inorganic forms of nitrogen, especially nitrates, can easily be leached because of ...200111724480
more qtl for flowering time revealed by substitution lines in brassica oleraceaseventy-nine recombinant backcross substitution lines from a cross between brassica oleracea var. italica and brassica oleracea var. alboglabra were grown in field trials over five years along with the alboglabra recurrent parent. plants were scored for the days from sowing to the opening of the first flower, and lines that flowered significantly earlier or later than the recurrent parent were identified. based on the lengths of the substitutions, evidence for 11 qtl on chromosomes o1, o2, o3, o ...199910620032
the construction of a substitution library of recombinant backcross lines in brassica oleracea for the precision mapping of quantitative trait loci.the currently available methods for locating quantitative trait loci (qtls) and measuring their effects in segregating populations lack precision unless individual qtls have very high heritabilities. the use of recombinant backcross lines containing short regions of donor chromosome introgressed into a constant recipient background permits qtls to be located with greater precision. the present paper describes the use of molecular markers to introgress defined short regions of chromosome from a d ...199618469917
a new class of s sequences defined by a pollen recessive self-incompatibility allele of brassica oleracea.self-incompatibility in brassica oleracea is controlled by a single genetic locus (the s locus) with nearly 50 different alleles. in this paper, we report the characterization of the s2 allele, a pollen recessive self-incompatibility allele that exhibits weak dna homology to the other previously sequenced s locus glycoprotein genes (slg-6, -13, -14, -22 from alleles s6, s13, s14 and s22, respectively). stigma cdna clones with sequence homology to slg-13 were isolated from two different s2 homozy ...19901980334
effects of phosphorus amendments and plant growth on the mobility of pb, cu, and zn in a multi-metal-contaminated soil.phosphorus amendments have been widely and successfully used in immobilization of one single metal (e.g., pb) in contaminated soils. however, application of p amendments in the immobilization of multiple metals and particularly investigations about the effects of planting on the stability of the initially p-induced immobilized metals in the contaminated soils are far limited.201222161145
cytology, rapd, and seed colour of progeny plants from brassica rapa-alboglabra aneuploids and development of monosomic addition lines.progeny plants from brassica rapa-alboglabra aneuploids were characterized genetically by scoring random amplified polymorphic dna (rapd) markers and seed colour and cytologically as to chromosome number and pairing. sets of rapd markers specific for each of the encountered eight alien brassica alboglabra chromosomes were defined. the finding of subsets of markers associated with the presence or absence of alien chromosomes inferred the frequent occurrence of intergenomic genetic recombination a ...200111768204
brassica taxonomy based on nuclear restriction fragment length polymorphisms (rflps) : 2. preliminary analysis of subspecies within b. rapa (syn. campestris) and b. oleracea.preliminary analysis using nuclear rflps provided evidence that subspecies within brassica rapa originated from two different centers. one center is in europe, represented by turnip and turnip rape from which the oilseed sarson was derived. a second center is in south china containing a variety of chinese vegetables of which pak choi and narinosa seem to be the most ancient forms. based on rflp data, the accessions of b. oleracea examined could be divided into three distinct groups, represented ...198824232282
food safety in thailand 4: comparison of pesticide residues found in three commonly consumed vegetables purchased from local markets and supermarkets in thailand.the wide use of pesticides raises concerns on the health risks associated with pesticide exposure. for developing countries, like thailand, pesticide monitoring program (in vegetables and fruits) and also the maximum residue limits (mrl) regulation have not been entirely implemented. the mrl is a product limit, not a safety limit. the mrl is the maximum concentration of a pesticide residue (expressed as mg/kg) recommended by the codex alimentarius commission to be legally permitted in or on food ...027635366
potential use of sludge cake from paper mill wastewater treatment as degradable flower pot.sludge cake produced in paper mill industries is disposed into a landfill and may cause the environmental and health problems. now many researchers have tried to recycle rigid materials from it for the purpose of decreasing its volume. the aims of this study were to clarify three hypotheses: (1) whether a flower pot would be economically made from sludge cake, (2) whether it would be safe for environment, and (3) when vegetables would grow enough in it, whether they would be safe for human consu ...026961595
cytological and morphological analysis of hybrids between brassicoraphanus, and brassica napus for introgression of clubroot resistant trait into brassica napus l.interspecific hybridization is a powerful tool for improvement of crop species, it has the potential to broaden the genetic base and create new plant forms for breeding programs. synthetic allopolyploid is a widely-used model for the study of genetic recombination and fixed heterosis in brassica. in brassica napus breeding, identification and introgression of new sources of clubroot resistance trait from wild or related species into it by hybridization is a long-term crop management strategy for ...201728505203
effects of glucose and gibberellic acid on glucosinolate content and antioxidant properties of chinese kale sprouts.glucosinolates, anthocyanins, total phenols, and vitamin c, as well as antioxidant capacity, were investigated in chinese kale sprouts treated with both glucose and gibberellic acid (ga3). the combination of 3% (0.03 g/ml) glucose and 5 μmol/l ga3 treatment was effective in increasing glucosinolate content while glucose or ga3 treatment alone did not influence significantly almost all individual glucosinolates or total glucosinolates. the total phenolic content and antioxidant activity of chines ...201829204989
evaluation of the nutritional quality of chinese kale (brassica alboglabra bailey) using uhplc-quadrupole-orbitrap ms/ms-based metabolomics.chinese kale (brassica alboglabra bailey) is a widely consumed vegetable which is rich in antioxidants and anticarcinogenic compounds. herein, we used an untargeted ultra-high-performance liquid chromatography (uhplc)-quadrupole-orbitrap ms/ms-based metabolomics strategy to study the nutrient profiles of chinese kale. seven chinese kale cultivars and three different edible parts were evaluated, and amino acids, sugars, organic acids, glucosinolates and phenolic compounds were analysed simultaneo ...201728749430
molecular characterization of myb28 involved in aliphatic glucosinolate biosynthesis in chinese kale (brassica oleracea var. alboglabra bailey).glucosinolates are brassicaceae-specific secondary metabolites that act as crop protectants, flavor precursors, and cancer-prevention agents, which shows strong evidences of anticarcinogentic, antioxidant, and antimicrobial activities. myb28, the r2r3-myb28 transcription factor, directly activates genes involved in aliphatic glucosinolate biosynthesis. in this study, the myb28 homology (boamyb28) was identified in chinese kale (brassica oleracea var. alboglabra bailey). analysis of the nucleotid ...201728680435
de novo transcriptome assembly of chinese kale and global expression analysis of genes involved in glucosinolate metabolism in multiple tissues.chinese kale, a vegetable of the cruciferous family, is a popular crop in southern china and southeast asia due to its high glucosinolate content and nutritional qualities. however, there is little research on the molecular genetics and genes involved in glucosinolate metabolism and its regulation in chinese kale. in this study, we sequenced and characterized the transcriptomes and expression profiles of genes expressed in 11 tissues of chinese kale. a total of 216 million 150-bp clean reads wer ...201728228764
influence of pre-harvest red light irradiation on main phytochemicals and antioxidant activity of chinese kale sprouts.the effects of pre-harvest red light irradiation on main healthy phytochemicals as well as antioxidant activity of chinese kale sprouts during postharvest storage were investigated. 6-day-old sprouts were treated by red light for 24h before harvest and sampled for further analysis of nutritional quality on the first, second and third day after harvest. the results indicated that red light exposure notably postponed the degradation of aliphatic, indole, and total glucosinolates during postharvest ...201728041552
identification of mirnas affecting the establishment of brassica alboglabra seedling.micrornas (mirnas) are important for plant development including seed formation, dormancy, and germination, as well as seedling establishment. the brassica vegetable seedling establishment stage influences the development of high quality seedlings, but also affects the nutrient content of sprouts. chinese kale (brassica alboglabra) seedlings at different growth stages were used to construct two small-rna (srna) libraries. we comprehensively analyzed the mirnas in 2- and 9-day-old seedlings. an a ...201628018366
an efficient method for adventitious root induction from stem segments of brassica species.plant propagation via in vitro culture is a very laborious and time-consuming process. the growth cycle of some of the crop species is slow even in the field and the consistent commercial production is hard to maintain. enhanced methods of reduced cost, materials and labor significantly impact the research and commercial production of field crops. in our studies, stem-segment explants of brassica species were found to generate adventitious roots (ar) in aeroponic systems in less than a week. as ...201627446170
effects of light quality on main health-promoting compounds and antioxidant capacity of chinese kale sprouts.the effects of different light qualities, including white, red and blue lights, on main health-promoting compounds and antioxidant capacity of chinese kale sprouts were investigated using light-emitting diodes (leds) as a light source. our results showed that blue light treatment significantly decreased the content of gluconapin, the primary compound for bitter flavor in shoots, while increased the glucoraphanin content in roots. moreover, the maximum content of vitamin c was detected in the whi ...201626593611
[characterization of structural change of ascorbate peroxidase from chinese kale during denaturation by circular dichroism].circular dichroism (cd) is a special absorption spectrum. the secondary structure of protein such as α-helix, β-sheet and β-turn in the far ultraviolet region (190-250 nm) has a characteristic cd spectrum. in order to understand the activity and structural changes of ascorbate peroxidase from chinese kale (baapx) during denaturation, specific activity and percentage of secondary structure of baapx under different time, temperature and concentration were analyzed by cd dynamically. in addition, t ...201425752058
rdna genetic imbalance and nucleolar chromatin restructuring is induced by distant hybridization between raphanus sativus and brassica alboglabra.the expression of rdna in hybrids inherited from only one progenitor refers to nucleolar dominance. the molecular basis for choosing which genes to silence remains unclear. we report genetic imbalance induced by distant hybridization correlates with formation of rdna genes (nors) in the hybrids between raphanus sativus l. and brassica alboglabra bailey. moreover, increased ccgg methylation of rdna in f1 hybrids is concomitant with raphanus-derived rdna gene silencing and rdna transcriptional ina ...201525723542
generation and identification of brassica alboglabra-brassica campestris monosomic alien addition lines.four monosomic alien addition lines (maals) for brassica alboglabra-brassica campestris were developed through digenomic triploid (acc) backcrossing with the recurrent parent b. alboglabra (cc). the objectives of this study were to compare morphological traits, microsatellite markers (simple sequence repeats), chromosomal karyotypes, and meiotic behaviors. based on the new chromosome nomenclature system established for brassica, we preliminarily identified these maals as cc+a1, cc+a3, cc+a6, and ...201323659701
comparative analysis of peroxidase profiles in chinese kale (brassica alboglabra l.): evaluation of leaf growth related isozymes.plant peroxidases (ec 1.11.1.7) with different isoforms catalyze various reactions in plant growth and development. however, it is difficult to elucidate the function of each isozyme in one plant. here, we compared profiles of entire isozyme in young seedling and mature leaves of chinese kale (brassica alboglabra l.) on zymogram and ion exchange chromatography in order to investigate leaf growth related peroxidase isozymes. the results showed that four isozymes were constitutively expressed in k ...201323122107
characterization of pbdes in soils and vegetations near an e-waste recycling site in south china.the concentration and composition of pbdes in the soils and plants near a typical e-waste recycling site in south china were investigated. the total concentration of pbdes (σpbdes) in soil ranged from 4.8 to 533 ng/g dry wt. the σpbdes in vegetation were from 2.1 to 217 ng/g dry wt. for the vegetable, the highest concentration of 19.9 ng/g dry wt. was observed in the shoot of brassica alboglabra l. bde 209 was the predominant congener in all samples. in comparison with other e-waste contaminated ...201121763040
instability of chromosome number and dna methylation variation induced by hybridization and amphidiploid formation between raphanus sativus l. and brassica alboglabra bailey.distant hybridization can result genome duplication and allopolyploid formation which may play a significant role in the origin and evolution of many plant species. it is unclear how the two or more divergent genomes coordinate in one nucleus with a single parental cytoplasm within allopolyploids. we used cytological and molecular methods to investigate the genetic and epigenetic instabilities associated with the process of distant hybridization and allopolyploid formation, measuring changes in ...201020849584
toxic effects of heavy metals and their accumulation in vegetables grown in a saline soil.a pot experiment was carried out to evaluate the effects of heavy metals on biomass, chlorophyll, and antioxidative enzyme activities of eight vegetables grown in a saline soil. the heavy metal accumulation in vegetables was also investigated. results show that biomass and chlorophyll content of crops decreased with the increase of heavy metal concentration while peroxidase activity increased at low concentration and decreased at high concentration. the total translocation factor values in the e ...201019781777
identification of the phenolic components of collard greens, kale, and chinese broccoli.an lc-ms profiling method was used for a comprehensive study of the phenolic components of collard greens, kale, and chinese broccoli, three brassica green leafy vegetables. this study led to the identification of 45 flavonoids and 13 hydroxycinnamic acid derivatives in the three vegetables. most of the identifications were based on comparison of compounds previously reported in the literature for brassica vegetables. the results indicate that the three materials have very similar phenolic compo ...200919627150
effect of co2 enrichment on the glucosinolate contents under different nitrogen levels in bolting stem of chinese kale (brassica alboglabra l.).the effects of co(2) enrichment on the growth and glucosinolate (gs) concentrations in the bolting stem of chinese kale (brassica alboglabra l.) treated with three nitrogen (n) concentrations (5, 10, and 20 mmol/l) were investigated. height, stem thickness, and dry weights of the total aerial parts, bolting stems, and roots, as well as the root to shoot ratio, significantly increased as co(2) concentration was elevated from 350 to 800 microl/l at each n concentration. in the edible part of the b ...200919489111
enhancement effect study of some organic acids on the calcium availability of vegetables: application of the dynamic in vitro simulated gastrointestinal digestion method with continuous-flow dialysis.the effect of added organic acids on the calcium availability of vegetables was investigated using the dialysis profiles obtained from an in vitro simulated gastrointestinal digestion with continuous-flow dialysis method. citric acid was the most effective enhancer followed by tartaric, malic, and ascorbic acids. for amaranth, which has a low calcium availability (5.4%), a significant increase of availability was observed with increasing concentrations of all acids studied. with the continuous-f ...200617117785
[effects of environmental factors on phyllotreta striolata dispersion].using marking recapture method, this paper studied the effects of host plants and climatic factors on the dispersion of phyllotreta striolata adults. the results showed that the dispersion of the beetle was impelled by environment elements (tao = 1 ). temperature and wind speed had significantly positive correlations with the dispersion of the beetle (p < 0.05), while high temperature had a negative correlation. wind orientation had a significant effect on the dispersion of the beetle, with the ...200516180758
[effects of cruciferous vegetables on experimental population of diamondback moth plutella xylostella].the impact of four species of cruciferous vegetables on the experimental population of diamondback moth (dbm) plutella xylostella was evaluated in the laboratory. the results showed that there was no significant difference in total fecundity and longevity of dbm female moths reared on different crucifers. the population increase index of dbm reared on flowering chinese cabbage, chinese kale, leaf mustard, and pak-choi was 41.0, 69.5, 38.2 and 52.4, respectively, and the natural death at larvae s ...200314986376
comparative persistence of pesticides on selected cultivars of specialty vegetables.the behavior and dissipation of several pesticides on selected cultivars of specialty vegetable crops were compared to determine appropriate preharvest intervals for compliance with maximum residue limits. to reduce application variability, a tank mix of pesticides was applied for each trial. residues of eight pesticides applied to bok choi, chinese broccoli, and fuzzy squash were similar for the two leafy vegetables; residues were higher than on squash because of the latter's larger mass-to-sur ...200312590477
pyrethroid insecticide residues on vegetable crops.pyrethroid insecticides were applied on various vegetable crops as foliar treatments to determine dissipation rates. on chinese broccoli (guy lon), chinese mustard (pak choi) and chinese cabbage (kasumi, napa), fenvalerate was persistent with residues of 0.10, 0.14 and 0.11 mg kg-1, respectively, by day 21. cypermethrin residues on head lettuce were below 0.1 mg kg-1 by day 10 but on the leafier romaine and endive varieties it was more persistent and required 14-19 days to dissipate below this c ...200111517721
random amplified polymorphic dna markers of the brassica alboglabra chromosome of a b. campestris-alboglabra addition line.the alien c-genome chromosome in a brassica campestris alboglabra monosomic addition line was characterized by random amplified polymorphic dna (rapd) analysis. the alien chromosome carried three loci, ec, wc and lap-1c, controlling synthesis of erucic acid, white flower colour and a fast-migrating band of leucine aminopeptidase (lap-1cc) respectively. the rapd analysis revealed 17 markers specific to the alien chromosome. among 45 offspring plants from the selfed addition line the alien c-chrom ...19968785604
erratum: mitotic karyotypes of brassica campestris and brassica alboglabra and identification of the b. alboglabra chromosome in an addition line. 199518470209
mitotic karyotypes of brassica campestris and brassica alboglabra and identification of the b. alboglabra chromosome in an addition line.a brassica campestris-alboglabra monosomic addition line (genome: aa + one chromosome from the c genome, 2n = 21) harbours the brassica alboglabra (cc, 2n = 18) chromosome with the gene for erucic acid. in order to identify this chromosome, we have studied the mitotic prometaphase chromosomes of brassica campestris (aa, 2n = 20), b. alboglabra, and the monosomic addition line. more pronounced differential staining and size differences of chromosomes were observed in b. campestris than in b. albo ...199518470170
acute organophosphorus food poisoning caused by contaminated green leafy vegetables.an outbreak of food poisoning in which 105 persons from various parts of singapore were affected was traced to one type of green leafy vegetable (brassica alboglabra) that had been imported. the clinical symptoms were generally mild and the incubation period short (median 2.5 h). the etiology was confirmed when excessive levels of two organophosphorus pesticides--methamidophos (tamaron/monitor; 2.4 ppm-31.7 ppm) and profenofos (selecron/curacron; 1.1 ppm-5.4 ppm) were detected in the implicated ...20162386424
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