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comparative studies on polyguanylate polymerase and polyadenylate polymerase activities in the dna-dependent rna polymerase i fraction from cauliflower.the properties of poly(g) polymerase and poly(a) polymerase activities in the dna-dependent rna polymerase nucleosidetriphosphate: rna nucleotidyltransferase ec 2.7.7.6 i fraction from cauliflower (brassica oleracea var. botrytis) were comparatively investigated. the ph optimum, the effect of ionic strength, the effect of substrate concentration on the rate of synthesis, the effect of divalent metal ion concentration, and the time course of synthesis at different temperatures were all different ...19767554
[stimulatory action of brassica oleracea var. sabauda (savoy) on mycobacteria and mycobacterial infection (author's transl)].eleven vegetable species were investigated for the presence of growth-stimulating activity on mycobacteria. it was found that savoy juice (brassica oleracea var. sabauda), given orally, posessed a pronounced stimulating effect on m. tuberculosis, m. bovis, m. kansasii, m. avium and m. intracellulare serotype davis in the serum of experimental animals when tested in auxanograms (table 1). another 2 species, paprica (capsicum frutescens) and radish (raphanus sativus var. albus) showed only insigni ...1978746996
[action of the systemic fungicide dexon on several nadh dehydrogenases].the fungicide dexon (p-dimethylaminobenzenediazosulfonate, na-salt) inhibits the nadh oxidase activity of submitochondrial particles (etp) from beef heart (semi-inhibition concentration 1.4 mum), while the succinate oxidase activity is unaffected. measurements of the activity of several enzymatic partial reactions of the respiratory chain of etp suggest that dexon acts directly on the flavine of nadh dehydrogenase. soluble nadh-cytochrome c-oxidoreductase (mahler) and rotenone-insensitive nadh u ...1975823748
plant pyruvate dehydrogenase complex purification, characterization and regulation by metabolites and phosphorylation.the pyruvate dehydrogenase complex was purified from mitochondria of cauliflower, brassica oleracea var. botrytis floral buds to a specific activity of 5.4 mumol of nadh/min per mg of protein. the pyruvate dehydrogenase complex required coash, nad+, thiamine pyrophosphate and mg2+ for the oxidative decarboxylation of pyruvate. the kinetic analysis of the complex gave a series of parallel lines for all substrates. product interaction patterns showed that nadh is competitive with nad+; acetyl-coa ...1977922017
effects of tentoxin on enzymic activities in cucumber and cabbage cotyledons.tentoxin, produced by alternaria alternata (fr.) keissl. causes severe variegated chlorosis in germinating seedlings of certain dicotyledonous species. however, it does not impair radicle and hypocotyl elongation or cotyledon expansion. effects of the toxin on the activity of selected enzymes from both chloroplasts and cytoplasm were determined. cucumber (cucumis sativus l.), which is highly sensitive to tentoxin and cabbage (brassica oleracea l.), which is resistant, were used as test plants. t ...19751171367
purification and properties of dna topoisomerase i from broccoli.we have purified a topoisomerase activity from broccoli (brassica oleracea var. italica) to near homogeneity. the enzyme is an 80 kda monomer as judged by gel filtration chromatography and sds gel electrophoresis, though it may represent a proteolytic fragment of a larger protein. the enzyme is capable of removing both negative and positive supercoils in steps of one, does not absolutely require mg2+, is only very weakly stimulated by nacl, is inhibited by camptothecin, and cross-reacts with an ...19921316191
transformation of cauliflower (brassica oleracea l. var. botrytis)--an experimental survey.the paper compares different approaches for the genetic transformation of cauliflower (agrobacterium-mediated, peg-mediated and/or electroporation). transient expression of the neomycin phosphotransferase ii (nptii) gene could be detected after direct gene transfer. stable transformation was achieved using both agrobacterium-mediated and direct gene transfer. expression as well as incorporation of the nptii sequence could be demonstrated.19921320426
molecular characterization of type 1 serine/threonine phosphatases from brassica oleracea.we describe the isolation of cdna clones encoding type 1 serine/threonine protein phosphatase (pp1) from brassica oleracea stigmas. we demonstrate that pp1 form a multigene family in brassica. within their most conserved domain, these phosphatases are 80-90% identical at the amino acid level. one cdna (bopp1) was found to encode a protein that shows 78-80% sequence identity to maize, rabbit, and yeast pp1. the accumulation of bopp1 mrna is developmentally regulated. varying levels of bopp1-homol ...19921330067
evidence for cytochrome b5 as an electron donor in ricinoleic acid biosynthesis in microsomal preparations from developing castor bean (ricinus communis l.).the major b-type cytochrome in microsomal membrane preparations from developing endosperm of castor bean (ricinus communis) was cytochrome b5. cytochrome p-450 was also present. the microsomal membranes had delta 12-hydroxylase activity and catalysed the nad(p)h-dependent hydroxylation of oleate to yield ricinoleic acid. co had no effect on the hydroxylase activity. rabbit polyclonal antibodies were raised against the hydrophilic cytochrome b5 fragment purified from cauliflower (brassica olerace ...19921417766
sequence of the fifth largest subunit of rna polymerase ii from plants.an affinity-purified antibody raised against the fifth largest subunit of cauliflower (brassica oleracea) rna polymerase ii was used to screen an expression library and isolate an arabidopsis thaliana cdna clone. this cdna clone was used to isolate a soybean (glycine max) cdna clone, and both clones were sequenced. the open reading frames contain 176 amino acids and predict polypeptides of 19.5 and 19.6 kda for arabidopsis and soybean, respectively. the amino acid sequences of the arabidopsis an ...19921429663
monitoring and assessment of mercury pollution in the vicinity of a chloralkali plant. iv. bioconcentration of mercury in in situ aquatic and terrestrial plants at ganjam, india.in situ aquatic and terrestrial plants including a few vegetable and crop plants growing in and around a chloralkali plant at ganjam, india were analyzed for concentrations of root and shoot mercury. the aquatic plants found to bioconcentrate mercury to different degrees included marsilea spp., spirodela polyrhiza, jussiea repens, paspalum scrobiculatam, pistia stratiotes, eichhornia crassipes, hygrophila schulli, monochoria hastata and bacopa monniera. among wild terrestrial plants chloris barb ...19921536599
a major inducer of anticarcinogenic protective enzymes from broccoli: isolation and elucidation of structure.consumption of vegetables, especially crucifers, reduces the risk of developing cancer. although the mechanisms of this protection are unclear, feeding of vegetables induces enzymes of xenobiotic metabolism and thereby accelerates the metabolic disposal of xenobiotics. induction of phase ii detoxication enzymes, such as quinone reductase [nad(p)h:(quinone-acceptor) oxidoreductase, ec 1.6.99.2] and glutathione s-transferases (ec 2.5.1.18) in rodent tissues affords protection against carcinogens a ...19921549603
chloroplast dna topoisomerase i from cauliflower.an atp-independent dna topoisomerase has been isolated from chloroplasts of cauliflower leaves (brassica oleracea var. botrytis) through deae-cellulose, af-blue toyopearl, and hydroxyapatite column chromatography. the sedimentation coefficient and stokes radius of this enzyme are 3.6s and 3.6 nm, respectively, and the molecular weight of native enzyme is estimated to be 54,000. this enzyme changes the linking number in steps of one. the enzyme activity is stimulated by mgcl2, and this enzyme sho ...19911849883
cdna cloning and expression of brassica napus enoyl-acyl carrier protein reductase in escherichia coli.the onset of storage lipid biosynthesis during seed development in the oilseed crop brassica napus (rape seed) coincides with a drastic qualitative and quantitative change in fatty acid composition. during this phase of storage lipid biosynthesis, the enzyme activities of the individual components of the fatty acid synthase system increase rapidly. we describe a rapid and simple purification procedure for the plastid-localized nadh-dependent enoyl-acyl carrier protein reductase from developing b ...19911912503
the role of cytochrome b5 in delta 12 desaturation of oleic acid by microsomes of safflower (carthamus tinctorius l.).the electron donors for the membrane-bound fatty acid desaturases of higher plants have not previously been identified. in order to assess the participation of cytochrome b5 in microsomal fatty acid desaturation, the cytoplasmic domain of microsomal cytochrome b5 was purified from brassica oleracea, and murine polyclonal antibodies were prepared. the igg fraction from ascites fluid inhibited 62% of nadh-dependent cytochrome c reduction in safflower (carthamus tinctorius l.) microsomes. these ant ...19911989527
lack of fructose-1,6-bisphosphatase in a range of higher plants that store starch.the aim of this work was to discover whether fructose-1,6-bisphosphatase (fbpase) is present in higher-plant cells that synthesize storage starch. the following were examined: suspension cultures of soybean (glycine max), tubers of potato (solanum tuberosum), florets of cauliflower (brassica oleracea), developing endosperm of maize and of sweet corn (zea mays), roots of pea (pisum sativum), and the developing embryos of round and wrinkled varieties of pea. unfractionated extracts of each tissue ...19902173563
the s-locus specific glycoproteins of brassica accumulate in the cell wall of developing stigma papillae.self-incompatibility in brassica oleracea is now viewed as a cellular interaction between pollen and the papillar cells of the stigma surface. in this species, the inhibition of self-pollen occurs at the stigma surface under the influence of s-locus specific glycoproteins (slsg). we used antibodies specific for a protein epitope of slsg to study the subcellular distribution of these molecules in the stigmatic papillae. the antibodies have uncovered an interesting epitope polymorphism in slsg enc ...19892472986
effect of dietary brussels sprouts with increased selenium content on mammary carcinogenesis in the rat.brussels sprouts (brassica oleracea, l; jade cross e, hybrid cultivar) were cultivated with inorganic selenium added to the plant growth medium. sprague-dawley, female, weanling rats were divided into groups and fed 20% brussels sprouts diets containing either 0.03, 0.58, 1.29, or 6.71 ppm of selenium naturally occurring in the sprouts. these diets were fed 2 weeks before and 2 weeks after a single dose of 7,12-dimethylbenz[a]anthracene (dmba), and the rats were then placed on a low selenium bas ...19892496915
evaluation of the gastric antiulcerogenic effects of solanum nigrum, brassica oleracea and ocimum basilicum in rats.antiulcerogenic activities of three plant drugs were studied against aspirin-induced gastric ulcers in rats. in addition, their effects on output of gastric acid and pepsin and hexosamine concentrations in gastric fluid were recorded in ulcerated and non-ulcerated rats. solanum nigrum (aerial parts) powder and its methanolic extract decreased the ulcer index significantly. the activity may be due to inhibition of acid and pepsin secretions and/or their in vitro ability to bind these. brassica ol ...19892515396
effect of in vitro fermentation using human faecal inoculum on the water-holding capacity of dietary fibre.the water-holding capacities (whc) of four sources of fibre were measured using dialysis membranes and osmotic-suction pressures of 45, 89 and 178 mosmol/1 (1, 2 and 4 atm). at all pressures, pectin had the highest whc, followed by cabbage (brassica oleracea) and lucerne (medicago sativa) and then cellulose. a suction pressure of 89 mosmol/1 (2 atm) was used in the subsequent fermentation study since it had the lowest standard error of the mean and most closely approximated physiological conditi ...19852998439
the effects of dietary cabbage on xenobiotic-metabolizing enzymes and the binding of aflatoxin b1 to hepatic dna in rats.the effect of dietary cabbage (brassica oleracea) on the binding of aflatoxin b1 (afb1) to hepatic dna and on the activities of liver and intestinal microsomal and cytosolic enzymes was studied in weanling male fischer 344 rats. freeze-dried cabbage was fed to rats at a level of 25% in the diet for 21 days, while others received a basal diet. in the cabbage-fed group there was an 87% (p less than 0.01) reduction in the binding of afb1 to hepatic dna 2 hr after the ip injection of [3h]afb1 (3 mic ...19873114106
protective effect of dietary brussels sprouts against mammary carcinogenesis in sprague-dawley rats.the effect of dietary brussels sprouts (brassica oleracea, l.) on mammary carcinogenesis induced by 7,12-dimethylbenz[a]anthracene (dmba) was studied in sprague-dawley female rats. rats fed a 20% brussels sprouts diet only during the initiation period of carcinogenesis had a palpable mammary tumor incidence of 13%, while those fed a casein-cornstarch semi-purified diet during this initiation period had a tumor incidence of 77% after 15 weeks post dmba dose. when the rats were switched from the s ...19883129179
formation of n-nitroso compounds under simulated gastric conditions from kashmir foodstuffs.several foodstuffs and teas from an area of high esophageal cancer risk in kashmir (india) were studied under simulated gastric conditions with a realistic nitrite concentration for the formation of n-nitroso compounds. n-nitrosodimethylamine (ndma), n-nitrosoproline (npro), n-nitrosothiazolidine-4-carboxylic acid (ntca) and n-nitrosopipecolic acid (npic) were the main products in different foods. significant amounts of ndma were formed from dried fish (20 micrograms/kg), dried and pickled veget ...19883359419
the role of condensed tannins in the nutritional value of lotus pedunculatus for sheep. 4. sites of carbohydrate and protein digestion as influenced by dietary reactive tannin concentration.1. vegetative secondary growth lotus pedunculatus was cut daily, and fed fresh at hourly intervals (600 g dry matter (dm)/d) to three groups each of three sheep fitted with permanent cannulas into the rumen and duodenum. lotus fed to two of the groups was sprayed with low and high rates of polyethylene glycol (peg; molecular weight 3350), which specifically binds the condensed tannins (ct). nutrient intake and faecal excretion were measured directly, duodenal flows estimated from continuous intr ...19863663569
small tandemly repeated dna sequences of higher plants likely originate from a trna gene ancestor.several monomers (177 bp) of a tandemly arranged repetitive nuclear dna sequence of brassica oleracea have been cloned and sequenced. they share up to 95% homology between one another and up to 80% with other satellite dna sequences of cruciferae, suggesting a common ancestor. both strands of these monomers show more than 50% homology with many trna genes; the best homologies have been obtained with lys and his yeast mitochondrial trna genes (respectively 64% and 60%). these results suggest that ...19863774553
primary structure of cu-zn superoxide dismutase of brassica oleracea proves homology with corresponding enzymes of animals, fungi and prokaryotes.the complete amino-acid sequence of cu-zn superoxide dismutase from white cabbage (brassica oleracea) is reported. the polypeptide chain consists of 151 amino acids and has a molecular mass of 15,604 da. the primary structure of the reduced and s-carboxymethylated protein was determined by automated solid phase sequence analysis of tryptic fragments and peptides obtained by digestion with staphylococcus aureus proteinase v8. the protein shows a free amino terminus as was found for all non-mammal ...19863790249
responses to oral methionine supplementation in sheep fed on kale (brassica oleracea) diets containing s-methyl-l-cysteine sulphoxide.1. responses to twice-weekly oral supplementation with 4.0 g methionine were measured with lambs (27 kg) grazing kale (brassica oleracea) for 10 weeks (expt 1). in a second experiment with sheep fed on kale at hourly intervals, rumen fractional outflow rates of credta and ruthenium tris(1,10 phenanthroline) markers were measured from the rates of decline in their concentrations. rumen turnover of s-methyl-l-cysteine sulphoxide (smco) and of carbohydrate (cho) constituents were also measured. the ...19853870695
reducing the confounding effects of environment and diet on saliva thiocyanate values in ex-smokers.this study focuses on the use of saliva thiocyanate to corroborate self-report in 391 adult ex-smokers who claimed abstinence from cigarettes for a minimum of 3 months. results indicated significant thiocyanate confounders, such as using and inhaling nontobacco organic substances, working in a "smoky" environment (i.e., tobacco smoke), and consuming varieties of brassica oleracea (e.g., cabbage), that could be identified and adjusted for in order to clarify classification of smoking status. befo ...19854013869
some characteristics of ca2+ transport in plant mitochondria.coupled mitochondria isolated from the white leaves of cabbage (brassica oleracea, var. capitata) were inactive in respiration-coupled ca2+ accumulation, in contrast to mitochondria isolated from etiolated corn (zea mays) which showed the ability to take up ca2+ from the medium, although with a much lower activity than liver mitochondria. the addition of corn mitochondria to aerobic medium containing succinate as respiratory substrate and a free ca2+ concentration of 40 microm resulted in ca2+ u ...19854015659
endocrine regulation of metabolism in sheep given kale (brassica oleracea) and ryegrass (lolium perenne)-clover (trifolium repens) fresh-forage diets.diets of fresh kale (brassica oleracea) and ryegrass (lolium perenne)-clover (trifolium repens) herbage were fed to growing sheep in three experiments. in expts 1 and 3 the sheep were confined indoors and fed at hourly intervals, and all were given supplementary iodine to counteract kale goitrogens. lambs grazed the two forages for 24 weeks in expt 2, with and without intramuscular injections of iodized oil. the kale and herbage contained respectively 11 and less than 0.1 g s-methyl-l-cysteine s ...19854063301
glucose and protein metabolism during late pregnancy in triplet-bearing ewes given fresh forages ad lib. 1. voluntary intake and birth weight.ewes of the booroola x romney genotype carrying triplet lambs were given fresh forages ad lib. in late pregnancy. in expt 1, groups of three ewes were given kale (brassica oleracea), perennial ryegrass (lolium perenne) or perennial ryegrass (0.75)-barley (0.25). in expt 2, groups of two or three ewes were given fresh perennial ryegrass and infused into the abomasum with iso-energetic quantities of casein and glucose in a 2 x 2 factorial arrangement. post-lambing ewe live weights were 40-50 kg. g ...19854063334
[a low-molecular rotenone-like acting inhibitor of the nadh oxidase system in cauliflower buds (brassica oleracea l.)]. 19744152634
[action of respiratory inhibitors on mitochondrial respiratory enzymes in cauliflower (brassica oleracea l.)]. 19744152638
genetic control of quantitative variation in self-incompatibility proteins detected by immunodiffusion.single radial immunodiffusion was used to study the self-incompatibility (s) proteins present in stigmas of two inbred lines of brassica oleracea: a self-incompatible line and a self-compatible mutant derived from it. the genetic basis of observed quantitative differences in s proteins between the two inbreds was shown to be determined by a single gene with dosage effects. self-pollination of individual plants with high, intermediate and low levels of s protein in the stigmas, respectively, resu ...19744206458
the role of molybdenum in the synthesis of nitrate reductase in cauliflower (brassica oleracea l. var botrytis l.) and spinach (spinacea oleracea l.). 19744474017
[the pharmacological effects of extract of brassica oleracea var. capitata (red cabbage)]. 19715169696
the effects of tentoxin on chlorophyll synthesis and plastid structure in cucumber and cabbage.to determine if chlorosis caused by tentoxin, a toxin produced by alternaria tenuis nees., is due to interference with chlorophyll synthesis directly or to disruption of normal chloroplast development, the effects of the toxin on these processes in cucumber (cucumis sativus l.) and cabbage (brassica oleracea l., var. capitata) were studied. cucumber cotyledons are highly sensitive to the toxin but exhibited no interference with the conversion of protochlorophyll(ide) to chlorophyll(ide) or with ...19705463696
copper metabolism in growing sheep given kale (brassica oleracea) and ryegrass (lolium perenne)-clover (trifolium repens) fresh forage diets.kale (brassica oleracea) and ryegrass (lolium perenne)-clover (trifolium repens) pasture grown under similar soil conditions were grazed in the vegetative state by growing lambs of 23.6 kg initial live weight for 24 weeks. forty-eight lambs grazed each forage. the kale and pasture contained respectively 4 and 14 mg copper/kg dry matter (dm), 7.2 and 3.1 g total sulphur/kg dm and 0.4 and 1.1 mg molybdenum/kg dm. subcutaneous injections of cu (12 mg) were given to half the animals grazing each for ...19836311244
effect of dietary indole-3-carbinol on intestinal and hepatic monooxygenase, glutathione s-transferase and epoxide hydrolase activities in the rat.male sprague-dawley rats were fed on purified diets supplemented with 50-500 ppm indole-3-carbinol (i3c), a compound present in cruciferous vegetables, or with 25% brussels sprouts (brassica oleracea) for 10 days after a 1-wk equilibration on a purified diet. cytosolic and microsomal fractions were prepared from liver and intestinal mucosae. intestinal aryl hydrocarbon hydroxylase (ahh) activity was increased significantly (p less than 0.05) over basal levels by i3c at 50 ppm (a 6.1-fold increas ...19846334634
acute poisoning in cattle after eating old non-viable seed of chou moellier (brassica oleracea convar. acephala).thirty cows gained access to a heap of discarded seed of chou moellier that had been stored in a barn for 10 years. within 24 h 2 cows were dead and one was acutely ill. the outstanding lesion was oedema of the wall of the rumen. the toxicity was attributed to damage to blood vessels in the rumen wall resulting in marked loss of fluid.19836651686
persistence of carbaryl residues in and on unprocessed and processed cauliflower curds.carbaryl was applied three times at 15 days interval to cauliflower (brassica oleracea var. botrytis l.), and its persistence was determined spectrophotometrically at 0, 1, 3, 6, 10, and 15 days after the first and last treatments. in unprocessed cauliflower the prescribed tolerance level for carbaryl residues of 5 ppm was only reached on the sixth day, in processed cauliflower on the third day after application. pretreatment with washing + boiling/cooking on the other hand led to a considerable ...19826807280
iodine metabolism and thyroid hormone relationships in growing sheep fed on kale (brassica oleracea) and ryegrass (lolium perenne)-clover (trifolium repens) fresh-forage diets. 19836830751
hepatic polysubstrate monooxygenase activities in different strains of rats fed cabbage (brassica oleracea).weanling male fischer (f), long-evans (le), and sprague-dawley (sd) rats were fed a semi-purified basal diet or a 20% cabbage diet for 3 and 4 weeks. hepatic polysubstrate monooxygenase (psm) activity was measured. significant strain differences were found in aniline hydroxylase and p-nitrophenol o-demethylase activities. aniline hydroxylase and nadph cytochrome c reductase activities were significantly decreased in all strains after 4 weeks of cabbage dietary treatment. f and le rats consuming ...19836861663
characterization of two dna polymerases from cauliflower inflorescence.two distinct dna polymerases, a and b, isolated from a rapidly growing apical tissue of cauliflower (brassica oleracea var. botrytis) inflorescence have been characterized, and compared with dna polymerases, alpha and beta, from mouse myeloma. polymerase-a bears a strong resemblance to polymerase-alpha from mammalian cells in all properties examined. the character of polymerase-b is also quite similar to polymerase-beta of mammalian cells in chromatographic elution properties, template-primer ut ...19807390985
bacterial expression of the catalytic domain of 3-hydroxy-3-methylglutaryl-coa reductase (isoform hmgr1) from arabidopsis thaliana, and its inactivation by phosphorylation at ser577 by brassica oleracea 3-hydroxy-3-methylglutaryl-coa reductase kinase.the catalytic domain of 3-hydroxy-3-methylglutaryl-coa reductase isoform 1 (hmgr1cd) from arabidopsis thaliana has been expressed in escherichia coli in a catalytically active form and purified. the high efficiency of the bacterial expression system together with the simplicity of the purification procedure used in this study resulted in the attainment of large quantities of pure enzyme (about 5 mg/l culture) with a final specific activity of up to 17 u/mg. this specific activity is higher than ...19957588795
monoclonal antibody tem 106 reacts with a tonoplast intrinsic protein of 106 kda from brassica oleracea l.a monoclonal antibody, designated tem 106, that recognizes an intrinsic protein from the vacuole membrane (tonoplast) of cauliflower (brassica oleracea l. var. botrytis) is described. mice were immunized with a tonoplast fraction that had been purified from differentiating meristematic cells from the cauliflower head. hybridomas were generated and screened by means of enzyme linked immuno sorbent assays for differential reactivity to tonoplast over non-related proteins (bovine serum albumin). on ...19957615671
chemoprevention of azoxymethane-induced colon carcinogenesis by dietary feeding of s-methyl methane thiosulfonate in male f344 rats.modifying effects of dietary exposure of s-methyl methane thiosulfonate (mmts) isolated from cauliflower brassica oleracea l. var. botrytis on rat colon carcinogenesis induced by azoxymethane (aom) and on the expression of cell proliferation biomarkers were investigated in two experiments. in experiment 1, male f344 rats were given three s.c. injections of aom (15 mg/kg body weight) and fed 100 ppm mmts for 5 weeks, starting 1 week before the first dose of aom. the frequency of colonic aberrant ...19957664279
expression level of the slg gene is not correlated with the self-incompatibility phenotype in the class ii s haplotypes of brassica oleracea.in brassica, the s-locus glycoprotein (slg) gene has been strongly implicated in the self-incompatibility reaction. several alleles of this locus have been sequenced, and accordingly grouped as class i (corresponding to dominant s-alleles) and class ii (recessive). we recently showed that a self-compatible (sc) line of brassica oleracea expressed a class ii-like slg (slg-sc) gene. here, we report that the slg-sc glycoprotein is electrophoretically and immunochemically very similar to the recessi ...19957766870
purification and characterization of a dna strand transferase from broccoli.a protein with dna binding, renaturation, and strand-transfer activities has been purified to homogeneity from broccoli (brassica oleracea var italica). the enzyme, broccoli dna strand transferase, has a native molecular mass of at least 200 kd and an apparent subunit molecular mass of 95 kd and is isolated as a set of isoforms differing only in charge. all three activities are saturated at very low stoichiometry, one monomer per approximately 1000 nucleotides of single-stranded dna. strand tran ...19957784508
bioactive organosulfur phytochemicals in brassica oleracea vegetables--a review.sulfur-containing phytochemicals of two different kinds are present in all brassica oleracea (cruciferae) vegetables (cabbage, broccoli, etc.). they are glucosinolates (previously called thioglucosides) and s-methyl cysteine sulfoxide. these compounds, which are derived in plant tissue by amino acid biosynthesis, show quite different toxicological effects and appear to possess anticarcinogenic properties. glucosinolates have been extensively studied since the mid-nineteenth century. they are pre ...19957797181
molecular basis of the cauliflower phenotype in arabidopsis.genetic studies demonstrate that two arabidopsis genes, cauliflower and apetala1, encode partially redundant activities involved in the formation of floral meristems, the first step in the development of flowers. isolation of the cauliflower gene from arabidopsis reveals that it is closely related in sequence to apetala1. like apetala1, cauliflower is expressed in young flower primordia and encodes a mads-domain, indicating that it may function as a transcription factor. analysis of the cultivat ...19957824951
the gene for the major cuticular wax-associated protein and three homologous genes from broccoli (brassica oleracea) and their expression patterns.the gene for the major protein (wax9) found in surface wax of broccoli, designated wax9d, and three homologous genes (wax9a, b and c) were isolated from a genomic library using the previously isolated cdna encoding the wax9 protein as the probe; all four genes were sequenced. genomic southern blot analysis using the wax9 cdna as a probe showed the presence of at least four homologous genes in broccoli genome. the sequence of the originally isolated wax9 cdna matched with that of gene d. all four ...19957894511
evaluation of brassicas in grazing systems for sheep: i. quality of forage and animal performance.four years of grazing trials were conducted with brassica forages to evaluate their chemical composition and effect on adg of fattening lambs and breeding ewes in late fall. brassicas tested included kales (brassica oleracea l. var. acephala dc), turnips (b. rapa l.), and a chinese cabbage hybrid (b. rapa l. x b. pekinensis [lour.] rupr.). daily gains of lambs varied widely among years (19 to 330 g/d); adg on brassicas were, however, generally higher than on stockpiled kentucky 31 tall fescue (f ...19947928762
acholeplasma brassicae sp. nov. and acholeplasma palmae sp. nov., two non-sterol-requiring mollicutes from plant surfaces.two mollicutes (strains 0502t [t = type strain] and j233t), which were isolated from the surfaces of broccoli (brassica oleracea var. italica) plants or the crown tissues of the coconut palm (cocos nucifera), were capable of sustained growth in serum-free (or cholesterol-free) mycoplasma broth media. examination by electron and dark-field microscopic techniques revealed that the cells of each strain were small, nonhelical, nonmotile, pleomorphic, and coccoid and that each cell was surrounded by ...19947981098
molecular cloning of a cdna from brassica napus l. for a homologue of acyl-coa-binding protein.a cdna encoding an acyl-coa-binding protein (acbp) homologue has been cloned from a lambda gt11 library made from mrna isolated from developing seeds of oilseed rape (brassica napus l.). the derived amino acid sequence reveals a protein 92 amino acids in length which is highly conserved when compared with acbp sequences from yeast, cow, man and fruit fly. southern blot analysis of brassica napus genomic dna revealed the presence of 6 genes, 3 derived from the brassica rapa parent and 3 from bras ...19948075407
cloning and sequence analysis of a flo/lfy homologue isolated from cauliflower (brassica oleracea l. var. botrytis).a cdna library has been constructed from cauliflower curd in which floral development had been initiated. two cdnas (pbofh3 and pbofh8) have been isolated using the antirrhinum flo gene as a heterologous probe. the two clones were sequenced and found to contain introns. comparison of the deduced cdna sequence of bofh with flo and the arabidopsis homologue lfy reveals extensive homology. an mrna transcript of 1.6 kb appears on northern rna blots. this transcript can be detected, at low levels, be ...19938104529
a glutathione s-transferase (gst) isozyme from broccoli with significant sequence homology to the mammalian theta-class of gsts.a novel glutathione s-transferase (gst) was purified from broccoli (brassica oleracea var. italica). partial amino-acid sequencing indicated that the protein shared significant homology with several different plant gsts from maize, silene, dianthus, nicotiana and triticum, but little homology to yeast (issatchenkia) gst. one region of the polypeptide near the n-terminal also shared significant homology to a region of rat 5-5, rat 12-12 and human theta-gst (collectively referred to as the theta-g ...19948142481
changes on protein expression associated with salinity tolerance in brassica cell cultures.the synthesis of proteins from salt-tolerant brassica oleracea l. var. botrytis l. subvar. cauliflora (gars.) dc. (cauliflower) cell cultures is modified in relation to controls in several features. there are nine newly induced polypeptides in tolerant cultures (absent in control conditions). some of them are only present under low salt levels (85 mm nacl). another group seems to be representative of moderate and high salt levels (170 and 255 mm nacl), and a third group is present in all the sal ...19938220310
a plant receptor-like gene, the s-locus receptor kinase of brassica oleracea l., encodes a functional serine/threonine kinase.to investigate the catalytic properties of the brassica oleracea s-locus receptor kinase (srk), we have expressed the domain that is homologous to protein kinases as a fusion protein in escherichia coli. following in vivo labeling of cultures with 32p-labeled inorganic phosphate, we observed phosphorylation of the fusion protein on serine and threonine, but not on tyrosine. in contrast, labeling was not observed when lysine-524, a residue conserved among all protein kinases, was mutated to argin ...19938310048
an alternative transcript of the s locus glycoprotein gene in a class ii pollen-recessive self-incompatibility haplotype of brassica oleracea encodes a membrane-anchored protein.recent reports have shown that slg, one of two genes linked to the s locus of brassica, encodes a secreted glycoprotein. we have used rna gel blot analysis, genomic and cdna clone analysis, expression in transgenic plants, and immunodetection to characterize slg2, the slg gene derived from the s2 haplotype. this haplotype belongs to the class ii group of s haplotypes that exhibit a weak incompatibility phenotype and are pollen recessive. we showed that slg2 produces two transcript forms: the exp ...19938329897
s-methyl methane thiosulfonate, a new antimutagenic compound isolated from brassica oleracea l. var. botrytis.though various antimutagens with desmutagenic activities have been found in our daily foods of plant origin, the numbers of antimutagens with bio-antimutagenic activities found so far are limited. in the present study, a compound with potential bio-antimutagenic activity to escherichia coli b/r wp2 was newly isolated from cauliflower, brassica oleracea l. var. botrytis, and its chemical structure was identified to be s-methyl methane thiosulfonate by nmr and ms analysis.19938364458
characterization of rfrs9, a second member of the rhizobium fredii repetitive sequence family from the nitrogen-fixing symbiont r. fredii usda257.the genome of the nitrogen-fixing symbiont, rhizobium fredii usda257, contains nine copies of repetitive sequences known as the r. fredii repetitive sequence (rfrs) family. we previously sequenced rfrs3, which is linked to symbiosis plasmid-borne nodulation genes of this organism and has substantial homology to the t-dna of agrobacterium rhizogenes and lesser homology to reiterated sequences of bradyrhizobium japonicum. here we characterize a second family member, rfrs9. the ecori fragment conta ...19938382462
immunological evidence that hmg-coa reductase kinase-a is the cauliflower homologue of the rkin1 subfamily of plant protein kinases.three different antibodies against the rkin1 and bkin12 gene products from rye and barley recognized the 58 kda subunit of hmg-coa reductase kinase-a (hrk-a) from brassica oleracea on western blots. hrk-a was also detected by an antipeptide antibody in enzyme-linked immunoassays, and this was competed by the peptide antigen. hrk-a was not recognized by antibodies against plant, mammalian and saccharomyces cerevisiae relatives of rkin1, i.e. wheat pkaba1, rat amp-activated protein kinase and s. c ...19958543048
anti-hyperglycemic effect of some edible plants.the anti-hyperglycemic effect of 12 edible plants was studied on 27 healthy rabbits, submitted weekly to subcutaneous glucose tolerance tests after gastric administration of water, tolbutamide or a traditional preparation of the plant. tolbutamide, cucurbita ficifolia, phaseolus vulgaris, opuntia streptacantha, spinacea oleracea, cucumis sativus and cuminum cyminum decrease significantly the area under the glucose tolerance curve and the hyperglycemic peak. brassica oleracea var. botrytis, alliu ...19958569244
molecular analysis of two functional homologues of the s3 allele of the papaver rhoeas self-incompatibility gene isolated from different populations.the s3 allele of the s gene has been cloned from papaver rhoeas cv. shirley. the sequence predicts a hydrophilic protein of 14.0 kda, showing 55.8% identity with the previously cloned s1 allele, preceded by an 18 amino acid signal sequence. expression of the s3 coding region in escherichia coli produced a form of the protein, denoted s3e, which specifically inhibited s3 pollen in an in vitro bioassay. the recombinant protein was ca. 0.8 kda larger than the native stigmatic form, indicating post- ...19968639756
characterization of eds1, a mutation in arabidopsis suppressing resistance to peronospora parasitica specified by several different rpp genes.the interaction between arabidopsis and the biotrophic oomycete peronospora parasitica (downy mildew) provides an attractive model pathosystem to identify molecular components of the host that are required for genotype-specific recognition of the parasite. these components are the so-called rpp genes (for resistance to p. parasitica). mutational analysis of the ecotype wassilewskija (ws-0) revealed an rpp-nonspecific locus called eds1 (for enhanced disease susceptibility) that is required for th ...19968953768
functional analysis of a brassica oleracea slr1 gene promoter.the brassica oleracea s-locus-related gene 1 (slr1) is expressed in the papillar cells of brassica stigmas from a few days before anthesis. we have previously shown that a 1500-bp fragment of the slr1 gene promoter is sufficient to direct high-level, temporally regulated expression of the beta-glucuronidase reporter gene in the pistils of transgenic tobacco. we have carried out a deletion analysis of the slr1 promoter and found that elements required for pistil expression are located between -25 ...19968972601
s-methyl methanethiosulfonate, bio-antimutagen in homogenates of cruciferae and liliaceae vegetables.the isolation of a new type of bio-antimutagen, s-methyl methanethiosulfonate (mmts), from cauliflower (brassica oleracea var. botrytis) and the distribution and formation of mmts in cruciferae and liliaceae vegetables are described. for the separation and purification, cauliflower curds were homogenized, extracted with acetone, and then purified by organic solvent extraction and by various processes of chromatographic separation. the chemical structure of an active principle was identified as m ...19968987591
rapid induction by wounding and bacterial infection of an s gene family receptor-like kinase gene in brassica oleracea.a receptor-like kinase, srk, has been implicated in the autoincompatible response that leads to the rejection of self-pollen in brassica plants. srk is encoded by one member of a multigene family, which includes several receptor-like kinase genes with patterns of expression very different from that of srk but of unknown function. here, we report the characterization of a novel member of the brassica s gene family, sfr2. rna gel blot analysis demonstrated that sfr2 mrna accumulated rapidly in res ...19979014364
comparative analysis of the chromosomal and genomic organization of ty1-copia-like retrotransposons in pteridophytes, gymnosperms and angiosperms.we have investigated the physical distribution of the reverse transcriptase genes of ty1-copia-like retrotransposable elements from 12 plant species belonging to different subdivisions by hybridization in situ on chromosome preparations. ty1-copia-like elements showed different and non-random hybridization patterns. a dispersed distribution throughout most of the chromosomes with reduced hybridization at some regions or with some weak clustering at other regions was found in allium cepa, beta vu ...19979037155
floral homeotic gene expression defines developmental arrest stages in brassica oleracea l. vars. botrytis and italica.brassica oleracea l. vars, italica (broccoli) and botrytis (cauliflower) both undergo developmental arrests which result in heading phenotypes. we characterized these arrested tissues at the morphological and molecular levels, and defined the developmental changes that ensure after arrest has been broken. we found that the order of floral organ initiation and the positions of resulting floral organ primordia in this species in some respects from that of arabidopsis, which is a member of the same ...19979084216
multi-functional acetyl-coa carboxylase from brassica napus is encoded by a multi-gene family: indication for plastidic localization of at least one isoform.three genes coding for different multifunctional acetyl-coa carboxylase (accase; ec 6.4.1.2) isoenzymes from brassica napus were isolated and divided into two major classes according to structural features in their 5' regions: class i comprises two genes with an additional coding exon of approximately 300 bp at the 5' end, and class ii is represented by one gene carrying an intron of 586 bp in its 5' untranslated region. fusion of the peptide sequence encoded by the additional first exon of a cl ...19979096417
isolation of a cdna coding for l-galactono-gamma-lactone dehydrogenase, an enzyme involved in the biosynthesis of ascorbic acid in plants. purification, characterization, cdna cloning, and expression in yeast.l-galactono-gamma-lactone dehydrogenase (ec 1.3.2.3; gldase), an enzyme that catalyzes the final step in the biosynthesis of l-ascorbic acid was purified 1693-fold from a mitochondrial extract of cauliflower (brassica oleracea, var. botrytis) to apparent homogeneity with an overall yield of 1.1%. the purification procedure consisted of anion exchange, hydrophobic interaction, gel filtration, and fast protein liquid chromatography. the enzyme had a molecular mass of 56 kda estimated by gel filtra ...19979374475
a structure-specific endonuclease from cauliflower (brassica oleracea var. botrytis) inflorescence.a protein with structure-specific endonuclease activity has been purified to near homogeneity from cauliflower ( brassica oleracea var. botrytis) inflorescence through five successive column chromatographies. the protein is a single polypeptide with a molecular mass of 40 kda. using three different branched dna structures (flap, pseudo-y and stem-loop) we found that the enzyme, a cauliflower structure-specific endonuclease, cleaved the single-stranded tail in the 5'-flap and 5'-pseudo-y structur ...19979396804
a functional s locus anther gene is not required for the self-incompatibility response in brassica oleracea.the self-incompatibility (si) response in brassica involves recognition of self-pollen by the papillar cells of the stigma and is mediated by the products of genes localized at the s (self-incompatibility) locus. two s locus genes, srk and slg, are thought to encode components of a receptor complex present in the female partner. the putative gene product of sla, a third s locus-linked gene that is expressed specifically in anthers, is a candidate for the male component of the si recognition syst ...19979401128
tonoplast intrinsic proteins from cauliflower (brassica oleracea l. var. botrytis): immunological analysis, cdna cloning and evidence for expression in meristematic tissues.the vacuolar membrane (tonoplast) of plant cells contains aquaporins, protein channels that facilitate the selective transport of water. these tonoplast intrinsic proteins (tips) of 23-29 kda belong to the ancient major intrinsic protein (mip) family. a monospecific polyclonal antiserum directed against a 26 kda intrinsic protein from the tonoplast of meristematic cells from cauliflower (brassica oleracea l. var. botrytis) was used to screen a cdna library. two distinct cdnas have been isolated. ...19989530877
modification of enzyme sulfhydryl groups suppresses uv-induced mutagenesis depending on the nucleotide excision repair system in escherichia coli b/r wp2.s-methyl methanethiosulfonate (mmts), which was isolated from cauliflower (brassica oleracea var. botrytis) homogenate as a potent bio-antimutagen, has been used as an enzyme-sulfhydryl (sh) temporary blocking agent in modification studies of enzyme activities. we examined whether 23 kinds of mmts-related compounds have a suppressing effect on uv mutagenesis in escherichia coli b/r wp2. disulfide derivatives of diphenyl, 2.2'-dipyridine and 4.4'-dipyridine, and n-ethyl maleimide (nem), which tem ...19989539980
purification and characterization of a 100 kda dna polymerase from cauliflower inflorescence.a dna polymerase from cauliflower (brassica oleracea var. botrytis) inflorescence has been purified to near homogeneity through five successive column chromatographies, and temporally designated cauliflower polymerase 1. cauliflower polymerase 1 is a monopolypeptide with a molecular mass of 100 kda. the enzyme efficiently uses synthetic dna homopolymers and moderately activated dna and a synthetic rna homopolymer as template-primers. the enzyme is strongly sensitive to dideoxythymidine triphosph ...19989601087
tissue-related changes in methyl-esterification of pectic polysaccharides in cauliflower (brassica oleracea l. var. botrytis) stems.pectic substances are a major component of cell walls in vegetable plants and have an important influence on plant food texture. cauliflower (brassica oleracea l. var. botrytis) stem sections at different regions of the mature plant stem have been monitored for tissue-related changes in the native pectic polysaccharides. chemical analysis detected appreciable differences in the degree of methyl-esterification (me) of pectic polysaccharides. about 65% of galacturonic acid (galpa) residues were me ...19989640669
3-hydroxy-3-methylglutaryl-coenzyme a reductase kinase and sucrose-phosphate synthase kinase activities in cauliflower florets: ca2+ dependence and substrate specificities.plant 3-hydroxy-3-methylglutaryl-coa reductase(hmgr; ec 1.1.1.34) and sucrose-phosphate synthase (sps; ec 2.4.1.14) and synthetic peptides designed from the known phosphorylation sites of plant hmgr (sams*: kshmkynrstkdvk), rat acetyl-coa carboxylase (sams: hmrsamsglhlvkrr), spinach sps (sp2: grrjrrissvejjdkk), and spinach nadh:nitrate reductase (nr6: gptlkrtastpfjnttsk) were used to characterize kinase activities from cauliflower (brassica oleracea l. ) inflorescences. the three major peaks of ...19989675040
identification of glyoxalase i sequences in brassica oleracea and sporobolus stapfianus: evidence for gene duplication events.glyoxalase i (glxi) is the first of two enzymes involved in the cellular detoxification of methylglyoxal. a recent search of the national center for biotechnology information (ncbi) databases with the protein sequence of salmonella typhimurium glxi identified two new hypothetical proteins with unassigned function. these two sequences, from brassica oleracea and sporobolus stapfianus, have significant sequence similarity to known glxi sequences, suggesting that these two open reading frames encod ...19989694672
molecular cloning and functional expression of a water-soluble chlorophyll protein, a putative carrier of chlorophyll molecules in cauliflower.a cdna for a water-soluble chlorophyll (chl) protein (wscp) from cauliflower (brassica oleracea l. var botrys) was cloned and sequenced. the cdna contained an open reading frame encoding 19 residues for a signal peptide and 199 residues for the mature form of wscp. the sequence showed extensive homology to drought-stress-related, 22-kda proteins in some brassicaceae plants. functional wscp was expressed in escherichia coli as a fusion protein with a maltose-binding protein (mbp). when the recomb ...19989804827
structure, properties, and tissue localization of apoplastic alpha-glucosidase in crucifers.apoplastic alpha-glucosidases occur widely in plants but their function is unknown because appropriate substrates in the apoplast have not been identified. arabidopsis contains at least three alpha-glucosidase genes; aglu-1 and aglu-3 are sequenced and aglu-2 is known from six expressed sequence tags. antibodies raised to a portion of aglu-1 expressed in escherichia coli recognize two proteins of 96 and 81 kd, respectively, in vegetative tissues of arabidopsis, broccoli (brassica oleracea l.), a ...19999952433
arabidopsis sec21p and sec23p homologs. probable coat proteins of plant cop-coated vesicles.intracellular protein transport between the endoplasmic reticulum (er) and the golgi apparatus and within the golgi apparatus is facilitated by cop (coat protein)-coated vesicles. their existence in plant cells has not yet been demonstrated, although the gtp-binding proteins required for coat formation have been identified. we have generated antisera against glutathione-s-transferase-fusion proteins prepared with cdnas encoding the arabidopsis sec21p and sec23p homologs (atsec21p and atsec23p, r ...199910198103
localization of pyrophosphatase in membranes of cauliflower inflorescence cells.using a polyclonal antiserum specific for the tonoplastic h(+)-pyrophosphatase (tppase), significant amounts of antigenic polypeptides of the correct molecular mass were detected in western blots of plasma membrane isolated from cauliflower (brassica oleracea l.) inflorescence by phase-partitioning and subsequent sucrose density centrifugation. potassium iodide-stripped plasma membranes continued to give a strong positive signal, indicating that the ppase antigen detected was not a result of con ...199910333584
duplication of the brassica oleracea apetala1 floral homeotic gene and the evolution of domesticated cauliflower.development of the cauliflower phenotype in arabidopsis thaliana requires mutations at both the cauliflower and apetala1 loci. boap1 is the brassica oleracea orthologue to the arabidopsis ap1 gene, and is present in two copies in brassica genomes. the boap1-a gene appears to encode a full-length protein, but boap1-b alleles in b. oleracea contain insertions that lead to premature translation termination. the boap1-b allele found in most b. oleracea subspecies, including b. oleracea ssp. botrytis ...199910544496
differences in chlorophyll loss at 13 degrees c for two broccoli (brassica oleracea l.) cultivars associated with antioxidant enzyme activities.two broccoli cultivars which have been previously found to have large differences in yellowing susceptibility were studied to determine if there was a relationship between antioxidant capability and chlorophyll loss at a simulated retail display temperature. greenbelt cultivar retained a stable chlorophyll content over 4 days at 13 degrees c, while emperor cultivar showed a constant decline in chlorophyll content. these differences were not related to differences in water loss. superoxide dismut ...199810554190
isolation and structural elucidation of (13z,13'z,3r,3'r,6'r)-lutein from marigold flowers, kale, and human plasma.(13z,13'z,3r,3'r,6'r)-lutein has been isolated and purified from extracts of marigold flowers, fresh raw kale (brassica oleracea var. acephala), and human plasma and fully characterized by (1)h and (13)c nmr, uv/vis, and ms. while the concentration of (13z, 13'z)-lutein in kale and human plasma compared to (all-e,3r,3'r, 6'r)-lutein was found to be quite low, this compound was readily isolated by fractional crystallization of lutein from marigold extracts. thus, the mother liquors from two conse ...199910563916
the in vivo effect of a brassica oleracea var. capitata extract on ehrlich ascites tumors of mus musculus balb/c mice.an extract of brassica oleracea var. capitata juice was prepared using petroleum ether, ether, ethanol and an al2o3 column. the healing and tumor protecting effects of this extract were tested on ehrlich ascites (ea) solid tumors of mus musculus balb/c mice. complete disappearance of the tumors was observed in 54.5% of the animals in the experimental group (n = 22) which received 20 mg/day of the extract i.p. for 28 days. regression of the tumors (27%), fixation of tumor size (4%) and an increas ...199910707127
the exbd2 gene as well as the iron-uptake genes tonb, exbb and exbd1 of xanthomonas campestris pv. campestris are essential for the induction of a hypersensitive response on pepper (capsicum annuum).the tonb, exbb and exbd1 genes of xanthomonas campestris pv. campestris are essential for ferric iron uptake. in contrast, the exbd2 gene located in the same gene cluster is not essential. mutational analysis revealed that the ferric-iron-uptake genes tonb, exbb and exbd1 are necessary for the induction of a hypersensitive response (hr) on the nonhost plant pepper (capsicum annuum) and the induction of typical black rot symptoms on the host plant cauliflower (brassica oleracea). again, the exbd2 ...200010832632
variation and selection at the cauliflower floral homeotic gene accompanying the evolution of domesticated brassica oleracea.the evolution of plant morphologies during domestication events provides clues to the origin of crop species and the evolutionary genetics of structural diversification. the cauliflower gene, a floral regulatory locus, has been implicated in the cauliflower phenotype in both arabidopsis thaliana and brassica oleracea. molecular population genetic analysis indicates that alleles carrying a nonsense mutation in exon 5 of the b. oleracea cauliflower (bocal) gene are segregating in both wild and dom ...200010835404
a new method to monitor airborne inoculum of the fungal plant pathogens mycosphaerella brassicicola and botrytis cinerea.we describe a new microtiter immunospore trapping device (mtist device) that uses a suction system to directly trap air particulates by impaction in microtiter wells. this device can be used for rapid detection and immunoquantification of ascospores of mycosphaerella brassicicola and conidia of botrytis cinerea by an enzyme-linked immunosorbent assay (elisa) under controlled environmental conditions. for ascospores of m. brassicicola correlation coefficients (r(2)) of 0.943 and 0.9514 were obser ...200010877797
comparative mapping of quantitative trait loci sculpting the curd of brassica oleracea.the enlarged inflorescence (curd) of cauliflower and broccoli provide not only a popular vegetable for human consumption, but also a unique opportunity for scientists who seek to understand the genetic basis of plant growth and development. by the comparison of quantitative trait loci (qtl) maps constructed from three different f(2) populations, we identified a total of 86 qtl that control eight curd-related traits in brassica oleracea. the 86 qtl may reflect allelic variation in as few as 67 di ...200010924486
host-correlated morphological variation of myzus persicae (hemiptera: aphididae) populations in greece.morphological variation in nine characters of 157 clones of myzus persicae (sulzer) was examined by multivariate analysis. the clones were collected from peach, prunus persica, the primary host and the secondary hosts tobacco, nicotiana tabacum, cabbage, brassica oleracea, sugarbeet, beta vulgaris and pepper capsicum annuum. the 156 clones originated from various regions of greece, both in the north, where a large part of the population has an annual bisexual generation on peach, and in more sou ...200010996864
a comparison of peronospora parasitica (downy mildew) isolates from arabidopsis thaliana and brassica oleracea using amplified fragment length polymorphism and internal transcribed spacer 1 sequence analyses.amplified fragment length polymorphism (aflp) fingerprints and internal transcribed spacer 1 (its1) sequences from 27 peronospora parasitica isolates (collected from arabidopsis thaliana or brassica oleracea), 5 albugo candida isolates (from the same hosts and from capsella bursa-pastoris), and 1 bremia lactucae isolate (from lactuca sativa) were compared. the aflp analysis divided the isolates into five groups that correlated with taxonomic species and, in most cases, with host origin. the only ...200011017765
an osmotin-like cryoprotective protein from the bittersweet nightshade solanum dulcamara.cold acclimation in plants is a polygenic phenomenon involving increased expression of several genes. the gene products participate either directly or indirectly towards increasing cold tolerance. evidence of proteins having a direct effect on cold tolerance is emerging but limited. with isolated protoplasts from warm-grown kale (brassica oleracea) as a model system, we tested protein fractions from winter bittersweet nightshade, solanum dulcamara, stems for the presence of proteins that have a ...200011198420
ten isoenzymes of xyloglucan endotransglycosylase from plant cell walls select and cleave the donor substrate stochastically.to map the preferred cleavage sites of xyloglucan endotransglycosylases (xets; ec 2.4.1.207) along the donor substrate chain, we incubated the enzymes with tamarind (tamarindus indica) xyloglucan (donor substrate; approximately 205 kda; 21 microm) plus the nonasaccharide [(3)h]xllgol (gal(2).xyl(3).glc(3). [(3)h]glucitol; acceptor substrate; 0.6 microm). after short incubation times, to minimize multiple cleavages, the size of the (3)h-labelled transglycosylation products (determined by gel-perm ...200111311129
a brassica oleracea gene expressed in a variety-specific manner may encode a novel plant transmembrane receptor.the species brassica oleracea includes several agricultural varieties characterized by the proliferation of different types of meristems. using a combination of subtractive hybridization and pcr (polymerase chain reaction) techniques we have identified several genes which are expressed in the reproductive meristems of the cauliflower curd (b. oleracea var. botrytis) but not in the vegetative meristems of brussels sprouts (b. oleracea var. gemmifera) axillary buds. one of the cloned genes, termed ...200111333311
a novel gene mutation that confers abnormal patterns of beta-carotene accumulation in cauliflower (brassica oleracea var. botrytis).the or gene of cauliflower (brassica oleracea var. botrytis) causes many tissues of the plant to accumulate carotenoids and turn orange, which is suggestive of a perturbation of the normal regulation of carotenogenesis. a series of experiments to explore the cellular basis of the carotenoid accumulation induced by the or gene was completed. the or gene causes obvious carotenoid accumulation in weakly or unpigmented tissues such as the curd, pith, leaf bases and shoot meristems, and cryptically i ...200111359610
cloning and expression pattern of a gene encoding an alpha-xylosidase active against xyloglucan oligosaccharides from arabidopsis.an alpha-xylosidase active against xyloglucan oligosaccharides was purified from cabbage (brassica oleracea var. capitata) leaves. two peptide sequences were obtained from this protein, the n-terminal and an internal one, and these were used to identify an arabidopsis gene coding for an alpha-xylosidase that we propose to call atxyl1. it has been mapped to a region of chromosome i between markers at 100.44 and 107.48 cm. atxyl1 comprised three exons and encoded a peptide that was 915 amino acids ...200111402218
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