nematospora sinecauda sp. nov., a yeast pathogen of mustard undescribed yeast species was recovered from oriental (brassica juncea) and yellow (b. hirta) mustard seeds. the new species most closely resembled nematospora coryli but its asci were rarely cylindrical. the asci and ascospores of n. sinecauda were smaller and the spores did not possess a whip-like appendage. during germination a sprout cell formed first on the smooth anterior surface of the spore above the median ridge. the posterior region of the spore was decorated with interrupted concen ...19846524909
immunological evidence for accumulation of two high-molecular-weight (104 and 90 kda) hsps in response to different stresses in rice and in response to high temperature stress in diverse plant genera.rice seedlings accumulate stainable amounts of the 104 and 90 kda polypeptides in response to high temperature stress. we have purified and raised highly specific polyclonal antisera against both of these polypeptides. in western blotting experiments, we find that these proteins are accumulated to different extents in rice seedlings subjected to salinity (nacl), water stress, low-temperature stress and exogenous abscisic acid application. these proteins also accumulated when rice seedlings grown ...19957579180
horizontal transfer in the phytopathogenic fungal genus leptosphaeria and host-range expansion.all isolates examined of the phytopathogenic fungus leptosphaeria maculans that are aggressive to brassica napus and brassica rapa have a repetitive element, lmr1. horizontal transfer of lmr1 to an isolate of a closely related species of leptosphaeria correlates with the expansion of the host range of this isolate to include brassica juncea.19957627459
haematological & histological studies after curry leaf (murraya koenigii) & mustard (brassica juncea) feeding in rats.whole curry leaf and mustard fed to rats at doses equal to normal human intake did not cause any adverse effect on food efficiency ratio (fer), red blood cell count (rbc), white blood cells (wbc), total count, differential counts or on the levels of blood constituents, like serum electrolytes, blood urea, haemoglobin, total serum protein, albumin-globulin ratio, fibrin level, glycosylated haemoglobin and the activity of glutamic oxaloacetic transaminase (got), glutamic pyruvic transaminase (gpt) ...19958543364
nutritional evaluation of low glucosinolate mustard meals (brassica juncea) in broiler diets.experiments were conducted to evaluate the nutritional value of meal derived from low glucosinolate cultivars of mustard (brassica juncea) in comparison to samples of canola meal (brassica napus, brassica rapa). samples of brassica seed (four b. juncea, one b. napus, and one b. rapa) were processed using laboratory procedures to produce oil-extracted meals, which were examined for composition (dm basis), and nutritional value for broiler chickens as judged by nutrient retention (amen, ileal prot ...19979276890
anti-oxidant effects of curry leaf, murraya koenigii and mustard seeds, brassica juncea in rats fed with high fat diet.status of lipid peroxidation was studied in rats induced high fat diet and some commonly used spices, viz. murraya koenigit and brassica juncea. the study revealed that these species alter the peroxidation (thiobarbituric acid reactive substances) level to a beneficial extent. histological studies also focus on modulation of hepatic functions to near normal level.19979315222
sub-cellular immunolocalization of the glucosinolate sinigrin in seedlings of brassica juncea.polyclonal rat antibodies were raised to a bovine serum albumin-sinigrin conjugate and used to immunolocalize sinigrin (2-propenylglucosinolate) in imbibed seeds and developing seedlings of brassica juncea. (l.) czern. sinigrin was localized to protein bodies in aleurone-like cells but shown to be absent from myrosin cells. double labelling techniques were used to co-localize both myrosinase (beta-thioglucoside glucohydrolase, ec and sinigrin. myrosin grains were labelled only with the ...19989763706
biochemical and immunochemical characterization of brassica juncea glyoxalase i.a homogenous preparation of glyoxalase i (s-lactoylglutathione-lyase, ec was obtained from brassica juncea seedlings. the enzyme is a heterodimer with 27,000 and 29,000 m(r) subunits and native m(r) of 56,000. the circular dichroic spectra of the protein showed characteristics of a distinctly helical protein, and magnesium affected the secondary structure. it is a zinc metalloenzyme. amino acid modification studies suggested the involvement of histidine residues in catalysis. apo-glyoxa ...19989887525
characterisation of a cdna encoding gamma-glutamylcysteine synthetase in medicago truncatula.a gamma-ecs cdna from medicago truncatula was isolated using an arabidopsis thaliana cdna as probe. the analysis of the amino acid sequence deduced from this cdna revealed 80% identity with the gamma-ecs from a. thaliana and brassica juncea and suggested a plastidial localisation for the enzyme. gamma-ecs activity and high level of gsh were detected in the gamma-ecs-deficient e. coli strain expressing a fusion protein containing the m. truncatula gamma-ecs protein. southern blot analysis suggest ...199910694062
a novel protein kinase from brassica juncea stimulated by a protozoan calcium binding protein. purification and partial characterization.a novel protein kinase (bjccabpk) from etiolated brassica juncea seedlings has been purified and partially characterized. the purified enzyme migrated on sds/page as a single band with an apparent molecular mass of 43 kda. the optimum ph for the kinase activity was 8.0. it was stimulated more than sixfold by the protozoa entamoeba histolytica calcium binding protein ehcabp (10.5 nm) but not by calmodulin (cam) when used at equimolar concentration. moreover the kinase also did not bind cam-sephar ...200010824102
selenium speciation in enriched and natural samples by hplc-icp-ms and hplc-esi-ms with perfluorinated carboxylic acid ion-pairing agents.selenium-enriched plants, such as hyperaccumulative phytoremediation plants (astragalus praleongus, 517 micrograms g-1 se, and brassica juncea, 138 micrograms g-1 se in dry sample), yeast (1200, 1922 and 2100, micrograms g-1 se in dry sample), ramp (allium tricoccum, 48, 77, 230, 252, 405 and 524 micrograms g-1 se in dry sample), onion (allium cepa, 96 and 140 micrograms g-1 se in dry sample) and garlic (allium sativum, 68, 112, 135, 296, 1355 micrograms g-1 se in dry sample) were analyzed by hp ...200010885064
enhanced tolerance of transgenic brassica juncea to choline confirms successful expression of the bacterial coda gene.brassica juncea cv. pusa jaikisan was transformed with the coda gene for choline oxidase from arthrobacter globiformis with an aim to introduce glycine betaine biosynthetic pathway, as it lacks any means to synthesize glycine betaine. western blot analysis revealed the presence of choline oxidase in the protein extract from the coda transgenic lines, demonstrating that the bacterial coda gene had been successfully transcribed and translated in transgenic lines. good activity of choline oxidase i ...200011074276
cadmium tolerance and accumulation in indian mustard is enhanced by overexpressing gamma-glutamylcysteine investigate rate-limiting factors for glutathione and phytochelatin (pc) production and the importance of these compounds for heavy metal tolerance, indian mustard (brassica juncea) was genetically engineered to overexpress the escherichia coli gshi gene encoding gamma-glutamylcysteine synthetase (gamma-ecs), targeted to the plastids. the gamma-ecs transgenic seedlings showed increased tolerance to cd and had higher concentrations of pcs, gamma-glucys, glutathione, and total non-protein thiol ...199910594104
methyl jasmonate induces expression of a novel brassica juncea chitinase with two chitin-binding domains.we have cloned a 1.3 kb brassica juncea cdna encoding bjchi1, a novel acidic chitinase with two chitin-binding domains that shows 62% identity to nicotiana tabacum chia1 chitinase. bjchi1 is structurally unlike chia1 that has one chitin-binding domain, but resembles chia5 chitinase uda1, the precursor of urtica dioica agglutinin: however there is only 36.9% identity between them. we propose that bjchi1 should be classified under a new class, chia7. the spacer and the hinge region of bjchi1 are p ...199910527425
characterization of plant growth promoting rhizobacteria isolated from polluted soils and containing 1-aminocyclopropane-1-carboxylate deaminase.fifteen bacterial strains containing 1-aminocyclopropane-1-carboxylate (acc) deaminase were isolated from the rhizoplane of pea (pisum sativum l.) and indian mustard (brassica juncea l.) grown in different soils and a long-standing sewage sludge contaminated with heavy metals. the isolated strains were characterized and assigned to various genera and species, such as pseudomonas brassicacearum, pseudomonas marginalis, pseudomonas oryzihabitans, pseudomonas putida, pseudomonas sp., alcaligenes xy ...200111547884
cloning sulfur assimilation genes of brassica juncea l.: cadmium differentially affects the expression of a putative low-affinity sulfate transporter and isoforms of atp sulfurylase and aps reductase.the heavy-metal accumulator brassica juncea l. is a high-biomass crop able to extract heavy-metal ions from the soil, a substantial part being translocated from root to shoot. previous work has shown that cd accumulation is accompanied by massive formation of phytochelatins (pcs). rapid de novo synthesis of pcs in roots and leaves requires an increased synthesis of the tripeptide glutathione (gsh), which in turn depends on increased sulfur assimilation. therefore. we have cloned cdnas for three ...199910350097
glyoxalase i from brassica juncea: molecular cloning, regulation and its over-expression confer tolerance in transgenic tobacco under stress.despite its ubiquitous presence, the role of glyoxalase i has not been well investigated in plants. in order to find out its physiological functions, we have cloned and characterized a cdna from brassica juncea encoding glyoxalase i (gly i) and made transgenic tobacco plants harbouring gly i in both sense and antisense orientation. the transgenic nature of the plants was confirmed by southern blotting, and the estimated number of genes inserted ranged from one to six. the transcript and protein ...199910205896
overexpression of glutathione synthetase in indian mustard enhances cadmium accumulation and tolerancean important pathway by which plants detoxify heavy metals is through sequestration with heavy-metal-binding peptides called phytochelatins or their precursor, glutathione. to identify limiting factors for heavy-metal accumulation and tolerance, and to develop transgenic plants with an increased capacity to accumulate and/or tolerate heavy metals, the escherichia coli gshii gene encoding glutathione synthetase (gs) was overexpressed in the cytosol of indian mustard (brassica juncea). the transge ...19999880348
production of 2,5-dioxopiperazine by a new isolate type of the blackleg fungus phoma isolates of the blackleg fungus [phoma lingam, perfect stage leptosphaeria maculans (desm.) ces. et de not.], virulent to the traditionally resistant brown mustard (brassica juncea), produce l-valyl-l-tryptophan anhydride, a 2,5-dioxopiperazine different from those produced by blackleg isolates virulent to canola (b. napus and b. rapa).199811711067
chemical defenses of crucifers: elicitation and metabolism of phytoalexins and indole-3-acetonitrile in brown mustard and turnip.the metabolism of the cruciferous phytoalexins brassinin and cyclobrassinin, and the related compounds indole-3-carboxaldehyde, glucobrassicin, and indole-3-acetaldoxime was investigated in various plant tissues of brassica juncea and b. rapa. metabolic studies with brassinin showed that stems of b. juncea metabolized radiolabeled brassinin to indole-3-acetic acid, via indole-3-carboxaldehyde, a detoxification pathway similar to that followed by the "blackleg" fungus (phoma lingam/leptosphaeria ...200211867093
distribution patterns of 104 kda stress-associated protein in rice.a 104 kda protein (sap 104) accumulates in rice seedlings in response to several abiotic stress conditions and immunological homologues of rice sap 104 have been detected in several monocot and dicot species, as also neurospora crassa, a fungus. in this report, we show that the amino acid sequence of a tryptic peptide generated from purified sap 104 bears significant homology with an atp-binding domain of the hsp 100 family proteins of arabidopsis thaliana and glycine max. it is further shown th ...19989700064
medicinal plants of india with anti-diabetic potential.since ancient times, plants have been an exemplary source of medicine. ayurveda and other indian literature mention the use of plants in treatment of various human ailments. india has about 45000 plant species and among them, several thousands have been claimed to possess medicinal properties. research conducted in last few decades on plants mentioned in ancient literature or used traditionally for diabetes have shown anti-diabetic property. the present paper reviews 45 such plants and their pro ...200212020931
precursor of the inactive 2s seed storage protein from the indian mustard brassica juncea is a novel trypsin inhibitor. charaterization, post-translational processing studies, and transgenic expression to develop insect-resistant plants.a number of trypsin inhibitor (ti) genes have been used to generate insect-resistant plants. here we report a novel trypsin inhibitor from indian mustard brassica juncea (bjti) that is unique in being the precursor of a 2s seed storage protein. the inhibitory activity is lost upon processing. the predicted amino acid sequence of the precursor based on the b. juncea 2s albumin (bj2s) gene cloned and sequenced in this laboratory (bj2sc; genbank(tm) accession number ) showed a soybean-ti active sit ...200212147695
a urinary biomarker for uptake of dietary isothiocyanates in humans.isothiocyanates (itcs) are a family of biologically active compounds that are distributed widely in cruciferous vegetables. although studies in rodents have shown that these compounds are effective and versatile inhibitors of tumorigenesis, the role of dietary itcs in the protection against human cancers remains to be established. a prerequisite of human studies is to develop an uptake biomarker for dietary itcs. in this study, we describe a rapid high-performance liquid chromatography-based ass ...19989488583
murraya koenigii and brassica juncea--alterations on lipid profile in 1-2 dimethyl hydrazine induced colon carcinogenesis.the influence of the two commonly used spices murraya koenigii (curry leaf) leaf and brassica juncea (mustard) seeds on the levels of lipids, fecal bile acids and neutral sterols was studied in rats administered 1,2-dimethyl hydrazine (1,2 dmh). the levels of cholesterol and phospholipids decreased in the experimental groups when compared with the control. the cholesterol phospholipid ratio showed an elevated level in the dmh treated control compared with the species group. bile acids and neutra ...19969157071
biochemical response in rats to the addition of curry leaf (murraya koenigii) and mustard seeds (brassica juncea) to the diet.three groups with 12 weanling male albino rats were group fed for 90 days on a standard laboratory rat diet plus 20% coconut oil either without a supplement or with the addition of 10% curry leaf or 10% mustard seeds. feed was offered at a level of 10% body weight. at the end of the trial, measurements were made on the total serum cholesterol, high density lipoproteins, low density lipoproteins, and very low density lipoproteins fractions, release of lipoproteins into the circulation, lecithin c ...19968983055
induction of tumours by indian dietary constituents.fishes and vegetables are preserved by salting and sundrying; and later fried in oil and consumed. such preparations have been found to contain polycyclic aromatic hydrocarbons, and are genotoxic and mutagenic. the potential carcinogenic effects of these and other dietary items have been studied by oral feeding to swiss mice at 100 mg/animal/day for 12 months; and observing upto two years. when so prepared and tested, chillies (capsicum annum l.) produce adenocarcinomas in the abdomen in 35% of ...19958772809
identification and quantification of the n-acetylcysteine conjugate of allyl isothiocyanate in human urine after ingestion of mustard.allyl isothiocyanate (aitc) is a constituent of cruciferous vegetables. it occurs widely in the human diet as a natural ingredient or food additive. aitc possesses numerous biochemical and physiological activities. it is cytotoxic and tumorigenic at high doses and also is a modulator of enzymes involved in metabolism of xenobiotics, including carcinogens. it is plausible that the wide consumption of dietary aitc may have profound effects on human health. to facilitate investigations of the effec ...19948000299
proline accumulates in plants exposed to uv radiation and protects them against uv induced peroxidation.proline accumulated in the shoots of seedlings of rice (oryza sativa), mustard (brassica juncea) and mung bean (vigna radiata) exposed to uv radiations. the level of proline in the seedlings increased significantly with increase in uv exposure time. the production of malondialdehyde (an indice of lipid peroxidation) was also higher in the shoots of seedlings exposed to uv radiation as compared to controls, suggesting that uv radiations promote lipid peroxidation. the extent of uv radiation promo ...19957726821
adsorption, complexation, and phytoavailability of copper as influenced by organic manure.copper (cu) is bound strongly to clay minerals and organic matter in soils, and forms both insoluble and soluble organic complexes with organic carbon. in this experiment, the effect of five manure composts (biosolid, farmyard manure, spent mushroom, pig manure, and poultry manure) on the adsorption and complexation of cu in a mineral soil (manawatu sandy soil, palmerston north, new zealand) low in organic matter content was examined. the effect of biosolid on the uptake of cu from the soil, tre ...200312558179
hypoglycemic action of murraya koenigii (curry leaf) and brassica juncea (mustard): mechanism of action.effect of murraya koenigii and brassica juncea on carbohydrate metabolism has been studied using rats as experimental animals. both showed significant hypoglycemic action. there was increase in the concentration of hepatic glycogen and glycogenesis, as evident from the increased activity of glycogen synthetase, and decrease in glycogenolysis and gluconeogenesis as evident from the decreased activity of glycogen phosphorylase and gluconeogenic enzymes.19957642200
degradation of sinigrin by lactobacillus agilis strain r16.forty-two lactobacilli were screened for their potential to degrade glucosinolate sinigrin. one of them, strain r16, demonstrated a high level of sinigrin degradation; it was identified as lactobacillus agilis. the sinigrin degrading activity of l. agilis r16 could only be demonstrated when intact cells were used. the products of sinigrin degradation are allyl-isothiocyanate (aitc) and glucose (which is further fermented to dl-lactic acid), suggesting that myrosinase activity is involved. the ac ...19957577359
effect of spiced food on metabolic rate.since the time of lavoisier it has been known that the ingestion of food in animals and man produces an increase in oxygen consumption. this increase in metabolic rate was originally called 'specific dynamic action' (sda) and is now widely referred to as the thermic effect (te) of food or diet-induced thermogenesis (dit) (rothwell & stock, 1981). much of the early work on the thermic effect was confined to the type and amount of food, notably the macronutrients--proteins, fats and carbohydrates. ...19863957721
isolation and characterization of a major allergen from oriental mustard seeds, braji.a 2 s albumin from oriental mustard (brassica juncea) seeds has been isolated and characterized as an allergen. this protein, braji, was recognized by human ige from mustard-sensitive individuals, as proved by using two different enzyme immunoassays. braji was found to be closely related to sin a i, the major allergen from yellow mustard seeds. many fractions with molecular weights ranging from 16,000 to 16,400 and with differences in charge were separated by ion-exchange chromatography. they ex ...19911804799
phosphatase activity in susceptible and resistant cultivars of brassica juncea inoculated with isolates of macrophomina phaseolina and sclerotinia sclerotiorum.inoculations with isolates of macrophomina phaseolina and sclerotinia sclerotiorum resulted in a significant increase in the acid phosphatase activity of susceptible and resistant cultivars of brassica juncea, which appeared to be related to the disease reaction of different host-pathogen combinations. after an increase on the 6th day of inoculation there was usually a fall in activity on the 12th and 18th days. resistant cultivars showed very poor activity in comparison to their susceptible cou ...1979542131
effect of iodine level in mustard (brassica juncea) cake-based concentrate supplement on nutrient utilisation and serum thyroid hormones of assess the influence of dietary iodine (i) supplementation on nutritional performance and serum thyroid hormones of goats, 12 adult barbari goats (average weight 18.8kg) were assigned randomly to three dietary treatments. the goats were fed a mustard (brassica juncea) cake containing concentrate supplement along with either 0mg (control, i(0)), 0.050mg (i(50)), or 0.075mg (i(75)) i per animal per day for 180 days. oat hay was given ad libitum as basal roughage. there was no difference in inta ...200111423234
identification of a delta 4 fatty acid desaturase from thraustochytrium sp. involved in the biosynthesis of docosahexanoic acid by heterologous expression in saccharomyces cerevisiae and brassica juncea.the existence of delta 4 fatty acid desaturation in the biosynthesis of docosahexanoic acid (dha) has been questioned over the years. in this report we describe the identification from thraustochytrium sp. of two cdnas, fad4 and fad5, coding for delta 4 and delta 5 fatty acid desaturases, respectively. the delta 4 desaturase, when expressed in saccharomyces cerevisiae, introduced a double bond at position 4 of 22:5(n-3) and 22:4(n-6) resulting in the production of dha and docosapentanoic acid. t ...200111397798
effect of substitution of groundnut with high glucosinolate mustard (brassica juncea) meal on nutrient utilization, growth, vital organ weight and blood composition of lambs.twenty-four 14-day-old weaner avivastra (russian merinoxnali) male lambs were maintained for 180 days on ad libidum cenchrus (cenchrus ciliaris) hay and concentrate mixture (cm) contained groundnut meal (control) and mustard meal (mm group) as major protein source. the two cms were isonitrogenous (21% cp) and isocaloric (2.78mcalmekg(-1) dm), while, cm fed to mm group contained 24.6mg glucosinolatesg(-1) dm. digestibility of nutrients was similar (p>0.05) in the two groups except for cp and hemi ...200111230962
mitochondrial 3-hydroxy-3-methylglutaryl-coa synthase deficiency: clinical course and description of causal mutations in two patients.hereditary deficiency of mitochondrial hmg-coa synthase (mhs, omim 600234) is a poorly defined, treatable, probably underdiagnosed condition that can cause episodes of severe hypoketotic hypoglycemia. we present clinical follow-up and molecular analysis of the two known mhs-deficient patients. the diagnosis of mhs deficiency is challenging because the symptoms and metabolite pattern are not specific. moreover, enzyme analysis is technically difficult and requires sampling of an expressing organ ...200111228257
in planta sequential hydroxylation and glycosylation of a fungal phytotoxin: avoiding cell death and overcoming the fungal facilitate plant colonization, some pathogenic fungi produce phytotoxic metabolites that damage tissues; plants may be resistant to a particular pathogen if they produce an enzyme(s) that catalyzes detoxification of this metabolite(s). alternaria blackspot is one of the most damaging and significant fungal diseases of brassica crops, with no source of resistance known within the brassica species. destruxin b is the major phytotoxin produced by the blackspot-causing fungus, alternaria brassica ...200111149945
expression of brassica juncea 3-hydroxy-3-methylglutaryl coa synthase is developmentally regulated and stress-responsive.3-hydroxy-3-methylglutaryl-coenzyme a synthase (hmgs) is an enzyme in mevalonate biosynthesis. in plants, investigations have focused on hmg coa reductase (hmgr) and less is known of the preceding enzyme, hmgs. to understand the regulation of hmgs, we have isolated a brassica juncea cdna encoding hmgs, bjhmgs1, for use as a hybridization probe in northern blot analyses. bjhmgs is expressed in all plant organs and shows developmental regulation in flower, seed and seedling, with highest expressio ...200010849357
characterisation of a cyanide hydratase gene in the phytopathogenic fungus leptosphaeria maculans.a gene encoding a cyanide hydratase was cloned from an aggressive isolate of leptosphaeria maculans, the fungus which causes blackleg disease of oilseed brassica spp. this enzyme catalyses the breakdown of hydrogen cyanide to a less toxic compound, formamide. the predicted amino acid sequence of cyanide hydratase in l. maculans is 77% and 82% identical to cyanide hydratases from two other ascomycetes, gloeocercospora sorghi and fusarium lateritium, respectively. the gene is present as a single c ...200010821180
functional activity and role of cation-efflux family members in ni hyperaccumulation in thlaspi goesingense.the ability of thlaspi goesingense to hyperaccumulate ni seems to be governed in part by enhanced accumulation of ni within leaf vacuoles. we have characterized genes from t. goesingense encoding putative vacuolar metal ion transport proteins, termed metal tolerance proteins (tgmtps). these proteins contain all of the features of cation-efflux family members, and evidence indicates they are derived from a single genomic sequence (tgmtp1) that gives rise to an unspliced (tgmtp1t1) and a spliced ( ...200111481436
effects of ddt on the growth of crop plants.the effects of ddt on the germination and growth of plants were studied using many crop species. of the species tested, oil-rich seeds of plants, such as peanut (arachis hypogaea) and mustard (brassica juncea), were more prone to ddt induced inhibition of germination and subsequent plant growth than cereals, pulses and fibre crops, like rice (oryza sativa), barley (hordeum vulgare), mung bean vigna radiata), pigeon pea (cajanus cajan) and cotton (gossypium hirsutum). studies with (14)c labelled ...198915092369
brassica juncea (rai) significantly prevented the development of insulin resistance in rats fed fructose-enriched diet.brassica juncea (bj; hindi name: rai) seeds and murraya koenigii (mk; english names: curry leaves) leaves, used as food ingredients and also by diabetics in india, were assessed in a fructose-mediated non-genetic model of insulin resistance. feeding of fructose rich diet for 30 days resulted in rise in blood glucose by 29.4%, insulin by 101.2% and cholesterol by 26.7% indicating development of insulin resistance. however, feeding of a fructose diet containing 10% brassica juncea seeds powder for ...200415182915
brassica juncea 3-hydroxy-3-methylglutaryl (hmg)-coa synthase 1: expression and characterization of recombinant wild-type and mutant enzymes.3-hydroxy-3-methylglutaryl (hmg)-coa synthase (hmgs; ec is the second enzyme in the cytoplasmic mevalonate pathway of isoprenoid biosynthesis, and catalyses the condensation of acetyl-coa with acetoacetyl-coa (acac-coa) to yield s-hmg-coa. in this study, we have first characterized in detail a plant hmgs, brassica juncea hmgs1 (bjhmgs1), as a his6-tagged protein from escherichia coli. native gel electrophoresis analysis showed that the enzyme behaves as a homodimer with a calculated ma ...200415233626
atopt6 transports glutathione derivatives and is induced by primisulfuron.the oligopeptide transporter (opt) family contains nine members in arabidopsis. while there is some evidence that atopts mediate the uptake of tetra- and pentapeptides, opt homologs in rice (oryza sativa; osgt1) and indian mustard (brassica juncea; bjgt1) have been described as transporters of glutathione derivatives. this study investigates the possibility that two members of the atopt family, atopt6 and atopt7, may also transport glutathione and its conjugates. complementation of the hgt1met1 ...200415247401
morphoanatomical response of two varieties of brassica juncea (l.) czern. grown on tannery sludge amended soil. 200415266700
suspected oriental mustard (brassica juncea) intoxication in cattle. 200111548963
probing host-selective phytotoxicity: synthesis and biological activity of phomalide, isophomalide, and dihydrophomalide.the cyclic depsipeptide phomalide [cyclo(val-(e)-aba-hpp-hmp-(r)-leu); aba = 2-amino-2-butenoic acid, hpp = (2s)-2-hydroxy-3-phenylpropanoic acid, hmp = (2s)-2-hydroxy-4-methylpentanoic acid] is the host-selective phytotoxin produced by the fungus [leptosphaeria maculans (desm.) ces. et de not., asexual stage phoma lingam (tode ex fr.) desm.] which causes blackleg disease (a devastating disease of several economically important brassica crops). efficient total syntheses of phomalide, its (z)-iso ...199911674233
isolation, structure determination, and phytotoxicity of unusual dioxopiperazines from the phytopathogenic fungus phoma lingam.the isolation, chemical structure elucidation, and bioactivity of polanrazines b-f, five new dioxopiperazines produced by isolates of the blackleg fungus [phoma lingam, perfect stage leptosphaeria maculans (desm.) ces. et de not.] originating from poland, are reported. polanrazines c and e showed moderate but selective toxicity, causing necrotic and chlorotic lesions (1-3 mm diameter) on brown mustard leaves.200111684188
high-level production of gamma-linolenic acid in brassica juncea using a delta6 desaturase from pythium irregulare.gamma-linolenic acid (gla), a nutritionally important fatty acid in mammals, is synthesized by a delta6 desaturase. here, we report identification of pid6, a new cdna from the oleaginous fungus, pythium irregulare, encoding a 459-amino acid protein that shares sequence similarity to carboxyl-directed desaturases from various species. expression of pid6 in yeast (saccharomyces cerevisiae) revealed that it converts exogenously supplied linoleic acid into gla, indicating that it encodes a delta6 fa ...200212011365
tobacco-expressed brassica juncea chitinase bjchi1 shows antifungal activity in vitro.we have previously isolated a brassica juncea cdna encoding bjchi1, a novel chitinase with two chitin-binding domains, and have shown that its mrna is induced by wounding and methyl jasmonate treatment (k.-j. zhao and m.-l. chye, plant mol. biol. 40 (1999) 1009-1018). by the presence of two chitin-binding domains, bjchi1 resembles the precursor of uda (urtica dioica agglutinin) but, unlike uda, bjchi1 retains its chitinase catalytic domain after post-translational processing. here, we indicate t ...200212175020
antioxidant effects of isorhamnetin 3,7-di-o-beta-d-glucopyranoside isolated from mustard leaf (brassica juncea) in rats with streptozotocin-induced investigate the effects of isorhamnetin 3,7-di-o-beta-d-glucopyranoside (isorhamnetin diglucoside), a major flavonoid compound of mustard leaf, on oxidative stress due to diabetes mellitus, in vivo and in vitro studies were carried out. oral administration of isorhamnetin diglucoside (10 or 20 mg/kg of body weight/day for 10 days) to rats with streptozotocin-induced diabetes significantly reduced serum levels of glucose and 5-(hydroxymethyl)furfural (5-hmf), which is glycosylated with hemoglo ...200212207497
immunopurification and immunocharacterization of the glucosinolate biosynthetic enzyme thiohydroximate s-glucosyltransferase.preparing homogeneous udp-glucose:thiohydroximate s-glucosyltransferase (s-gt), the penultimate biosynthetic enzyme of glucosinolates, by standard chromatographic methods has yielded too little protein for adequate purity evaluation, identity verification, and structural analysis. the low yields were apparently due to low abundance in source tissues, aggravated by enzyme instability. here we describe an immunological method for purification of workable quantities from florets of brassica olerace ...199412232212
role of murraya koenigii (curry leaf) and brassica juncea (mustard) in lipid peroxidation.the status of lipid peroxidation was investigated in rats fed m. koenigii (curry leaf) and b. juncea (mustard). concentration of malondialdehyde showed a significant decrease, while hydroperoxides and conjugated dienes were significantly increased in liver and heart of both the experimental groups. sod and catalase activity was found to be increased in liver and heart of both the spices administered groups. glutathione levels in liver, heart and kidney were lowered in rats administered these spi ...19969062811
peroxynitrite scavenging activity of sinapic acid (3,5-dimethoxy-4-hydroxycinnamic acid) isolated from brassica juncea.peroxynitrite (onoo(-)), formed from a reaction of superoxide and nitric oxide, is one of the most potent cytotoxic species that are known to oxidize cellular constituents including essential proteins, lipids, and dna. in this study, the ability of sinapic acid (3,5-dimethoxy-4-hydroxycinnamic acid), isolated from brassica juncea, to scavenge onoo(-) was investigated. the data obtained show that sinapic acid can efficiently scavenge native onoo(-) as well as onoo(-) derived from the peroxynitrit ...200212358454
isolation and characterization of a delta5 fa desaturase from pythium irregulare by heterologous expression in saccharomyces cerevisiae and oilseed using the polymerase chain reaction approach with two degenerate primers targeting the heme-binding and the third histidine-rich motifs in microsomal carboxyl-directed desaturases, we identified a cdna pid5 from pythium irregulare encoding a delta5 desaturase. the substrate specificity of the enzyme was studied in detail by expressing pid5 in a yeast (saccharomyces cerevisiae) mutant strain, amy-2alpha, where ole1, a delta9 desaturase gene, is disrupted. the result revealed that the encoded e ...200212458621
effects of organic amendments on the reduction and phytoavailability of chromate in mineral this study, seven organic amendments (biosolid compost, farm yard manure, fish manure, horse manure, spent mushroom, pig manure, and poultry manure) were investigated for their effects on the reduction of hexavalent chromium [chromate, cr(vi)] in a mineral soil (manawatu sandy soil) low in organic matter content. addition of organic amendments enhanced the rate of reduction of cr(vi) to cr(iii) in the soil. at the same level of total organic carbon addition, there was a significant difference ...200312549550
effect of feeding murraya koeingii and brassica juncea diet on [correction] kidney functions and glucose levels in streptozotocin diabetic mice.purpose of the study was to investigate the effects of daily oral feeding 15% of powdered leaves of murraya koeingii (mk) (commonly called as curry patta) and 10% powder of seeds of brassica juncea (bj) (commonly called as rai) for 60 days on serum glucose concentrations and kidney functions in streptozotocin (stz; 100mg/kg) diabetic rats. serum glucose levels, body weight, urine volume, serum creatinine, and urinary albumin (uae) levels were monitored on day 0, 10, 25, 40, and 70 of the experim ...200312576195
potential for rhizofiltration of uranium using hairy root cultures of brassica juncea and chenopodium amaranticolor.hairy root cultures of brassica juncea and chenopodium amaranticolor were developed by genetic transformation using agrobacterium rhizogenes. the stable, transformed root systems demonstrated a high growth rate of 1.5-3.0 g/g dry weight/day in murashige and skoog medium. in the present study, hairy root system was used for removal of uranium from the solution of concentration up to 5,000 microm. the results indicated that the hairy roots could remove uranium from the aqueous solution within a sh ...200312584014
bioengineering of a phytoremediation plant by means of somatic hybridization.phytoremediation is a technology that exploits a plant's ability to remove contaminants from the environment or render toxic compounds harmless. an efficient metal phytoremediating plant must combine high biomass production and established cultivation methods with high tolerance to a specific contaminant and ability for root uptake, translocation, and compartmentalization of contaminants in the above-ground biomass. symmetric and asymmetric somatic hybridizations were used to introduce toxic met ...200212655805
in vitro and in vivo antioxidant effects of mustard leaf (brassica juncea).to investigate the antioxidant activity of mustard leaf (brassica juncea), we prepared four fractions (ch(2)cl(2), etoac, buoh and h(2)o fractions) and examined their radical scavenging activities in vitro and in vivo. based on the in vitro results of spin trapping and 1,1-diphenyl-2-picrylhydrazyl radical, we carried out an in vivo study with the buoh fraction to investigate its effect on oxidative stress in rats with streptozotocin-induced diabetes. we found that in comparison with untreated d ...200312748980
cloning and characterization of a mitochondrial glyoxalase ii from brassica juncea that is upregulated by nacl, zn, and aba.a cdna (1061 bp) bj glyii was cloned from a mannitol induced library of brassica juncea. it encoded a protein of 335 amino acids with a molecular weight of 36.52 kda. the deduced amino acid sequence of the clone showed 92% and 56% identity with pennisetum and rice glyoxalase ii, respectively, and 30% identity was observed with the human glyoxalase ii. search for the identical residues revealed the presence of highly conserved thhhxdh domain which is involved in zinc binding. p-nn and psort analy ...200516153601
combined application of triton x-100 and sinorhizobium sp. pb002 inoculum for the improvement of lead phytoextraction by brassica juncea in edta amended soil.the process of edta-assisted lead phytoextraction from the bovisa (milan, italy) brownfield soil was optimized in microcosms vegetated with brassica juncea. an autochthonous plant growth-promoting rhizobacterium (pgpr), sinorhizobium sp. pb002, was isolated from the rhizosphere of b. juncea grown on the pb-contaminated soil in presence of 2 mm edta. the strain was augmented (10(8) cfu g(-1) soil) in vegetated microcosms to stimulate b. juncea biomass production and, hence, its phytoextraction po ...200616153689
protective effects of mustard leaf (brassica juncea) against diabetic oxidative stress.of four fractions (ch2cl2, etoac, buoh and h2o) from mustard leaf (brassica juncea), the etoac fraction showed the strongest inhibitory effects, which were concentration-dependent, on the formation of advanced glycation end products and free radical-mediated protein damage in an in vitro system, indicating that this fraction has a potential protective role against diabetes and/or its complications. based on these results, we carried out an in vivo study to determine whether the etoac fraction pr ...200312887153
modification of erucic acid content in indian mustard (brassica juncea) by up-regulation and down-regulation of the brassica juncea fat ty acid elongation1 (bjfae1) brassicas, the fatty acid elongation1 (fae1) gene product, a 3-ketoacyl-coa synthase, is the first in a 4-enzyme complex involved in the synthesis of erucic acid from oleic acid. the fae1 homologue from brassica juncea cv. pusa bold was cloned in a binary vector both in sense and antisense orientations under the control of the camv35s promoter. the recombinant binary vectors were used to transform b. juncea cv. rlm 198 via agrobacterium tumefaciens. the presence of the transgene was confirmed ...200616322995
heavy metal bioavailability in a soil affected by mineral sulphides contamination following the mine spillage at aznalcóllar (spain).a field experiment, lasting 14 months, was carried out in order to assess the effect of organic amendment and lime addition on the bioavailability of heavy metals in contaminated soils. the experiment took place in a soil affected by acid, highly toxic pyritic waste from the aznalcóllar mine (seville, spain) in april 1998. the following treatments were applied (3 plots per treatment): cow manure, a mature compost, lime (to plots having ph < 4), and control without amendment. during the study two ...200312889610
growth of brassica juncea under chromium stress: influence of siderophores and indole 3 acetic acid producing rhizosphere bacteria.plant growth promoting rhizobacterial (pgpr) strains a3 and s32 have been shown to promote the growth of brassica juncea under chromium stress which has been related to the microbial production of siderophores and indole 3 acetic acid (iaa). the aim of the present study is to evaluate the importance of siderophores and iaa producing pgpr on the growth of brassica juncea under chromium stress. the production of iaa and siderophores were observed in the strains a3 and s32, respectively. both pgpr ...200516459559
soil properties and crop productivity as influenced by flyash incorporation in soil.field experiments were carried out during 1996-97 at gulawathi, muthiani and salarpur villages, iari farm, new delhi and ncpp campus, dadri to evaluate changes in soil characteristics and growth of wheat (triticum aestivum l.), mustard (brassica juncea l.), lentil (lence esculenta moench.), rice (oryza sativa l.) and maize (zea mays l.) by varying amounts of flyash addition (up to 50t ha(-1)) in soils at sowing/transplanting time of crops. flyash addition in areas adjoining ncpp thermal power pl ...200312889638
the dual role of the potyvirus p3 protein of turnip mosaic virus as a symptom and avirulence determinant in brassicas.two isolates of the potyvirus turnip mosaic virus (tumv), uk 1 and cdn 1, differ both in their general symptoms on the susceptible propagation host brassica juncea and in their ability to infect b. napus lines possessing a variety of dominant resistance genes. the isolate cdn 1 produces a more extreme mosaic in infected brassica leaves than uk 1 and is able to overcome the resistance genes turb01, turb04, and turb05. the resistance gene turb03, in the b. napus line 22s, is effective against cdn ...200312971601
chitinase-mediated inhibitory activity of brassica transgenic on growth of alternaria brassicae.chitinase, capable of degrading the cell walls of invading phytopathogenic fungi, plays an important role in plant defense response, particularly when this enzyme is overexpressed through genetic engineering. in the present study, brassica plant (brassica juncea l.) was transformed with chitinase gene tagged with an overexpressing promoter 35 s camv. the putative transgenics were assayed for their inhibitory activity against alternaria brassicae, the inducer of alternaria leaf spot of brassica b ...200314570264
hydrophytes lack potential to exhibit cadmium stress induced enhancement in lipid peroxidation and accumulation of proline.investigations were carried out to evaluate if hydrophytes (viz. ceratophyllum, wolffia, and hydrilla) can be used as markers to assess the level of heavy metal pollution in aquatic bodies. the potential of these hydrophytes for lipid peroxidation and accumulation of proline in response to cadmium (cd2+) pollution was studied. hydrophytes were raised in artificial pond water (apw) supplemented with various levels of cd2+. interestingly, unlike mesophytes none of the hydrophytes showed ability to ...200415036869
an insight into the molecular basis of salt tolerance of l-myo-inositol 1-p synthase (pcino1) from porteresia coarctata (roxb.) tateoka, a halophytic wild rice.the molecular basis of salt tolerance of l-myo-inositol 1-p synthase (mips; ec from porteresia coarctata (roxb.) tateoka (pcino1, af412340) earlier reported from this laboratory, has been analyzed by in vitro mutant and hybrid generation and subsequent biochemical and biophysical studies of the recombinant proteins. a 37-amino acid stretch between trp-174 and ser-210 has been confirmed as the salt-tolerance determinant domain in pcino1 both by loss or gain of salt tolerance by either de ...200616500989
p-nitrosodimethylaniline (rno)-based evaluation of enhanced oxidative potential during electrochemical treatment of high-salinity wastewater.the electrochemical bleaching of p-nitrosodimethylaniline (rno) was investigated in order to apply its reactions to evaluating electrochemical oxidation. the bleaching rate of rno during electrochemical treatment was accelerated by the presence of chloride ions. the initial bleaching rate of rno was found to be accelerated during the electrolysis of nh4cl, suggesting that electrochemically generated chloramines are sufficiently strong oxidants to convert rno. a method of evaluating direct or ind ...200415055941
purification and characterization of a pma-stimulated kinase and identification of pma-induced phosphorylation of a polypeptide that is dephosphorylated by low temperature in brassica juncea.heterologous classical protein kinase c (cpkc) rat polyclonal antibodies showed presence of pkc homolog in brassica juncea seedlings. it was purified to homogeneity by ammonium sulfate precipitation, diethyl amino ethyl (deae)-sephacel, gel-filtration chromatography, and preparative gel electrophoresis. pkc-like kinase activity was fractionated into three distinct peaks after deae-sephacel chromatography. the kinase activity was associated with a 55 kda polypeptide. it was calcium dependent and ...200415325246
an agglutinating chitinase with two chitin-binding domains confers fungal protection in transgenic potato.brassica juncea bjchi1 is a unique chitinase with two chitin-binding domains. here, we show that, unlike other chitinases, potato-expressed bjchi1 shows hemagglutination ability. bjchi1 expression in b. juncea seedlings is induced by rhizoctonia solani infection, suggesting its protective role against this fungus. to verify this, transgenic potato (solanum tuberosum l. cv. desiree) plants expressing bjchi1 generated by agrobacterium-mediated transformation were challenged with r. solani. we also ...200515490228
selective detection and identification of se containing compounds--review and recent developments.the complexity of selenium (se) chemistry in the environment and in living organisms presents broad analytical challenges. the selective qualitative and quantitative determination of particular species of this element is vital in order to understand selenium's metabolism and significance in biology, toxicology, clinical chemistry and nutrition. this calls for state-of-the-art analytical techniques such as hyphenated methods that are reviewed with particular emphasis on interfaced separation with ...200415503929
chromatographic speciation of anionic and neutral selenium compounds in se-accumulating brassica juncea (indian mustard) and in selenized yeast.selenium-accumulating plants such as brassica juncea (indian mustard) concentrate the element in plant shoots and roots. such behavior may provide a cost-effective technology to clean up contaminated soils and waters that pose major environmental and human health problems (phytoremediation). such ability to transform selenium into bioactive compounds has important implications for human nutrition and health. element selective characterization of b. juncea grown in the presence of inorganic selen ...200415553157
functional analyses of the chitin-binding domains and the catalytic domain of brassica juncea chitinase bjchi1.we previously isolated a brassica juncea cdna encoding bjchi1, a novel chitinase with two chitin-binding domains. synthesis of its mrna is induced by wounding, methyl jasmonate treatment, aspergillus niger infection and caterpillar (pieris rapae) feeding, suggesting that the protein has a role in defense. in that it possesses two chitin-binding domains, bjchi1 resembles the precursor of urtica dioica agglutinin but unlike that protein, bjchi1 retains its chitinase catalytic domain after post-tra ...200415604744
a simplified method for cloning of short interfering rnas from brassica juncea infected with turnip mosaic potyvirus and turnip crinkle carmovirus.rna silencing is a plant defense mechanism in which virus infected plants produce short interfering rnas (sirnas) derived from viral rna, that attack the virus at the post-transcriptional level. in a previous study on cymbidium ringspot tombusvirus (cymrsv) infection in nicotiana benthamiana, sirnas (determined by cloning and sequencing) predominantly originated from the sense (+) strand of the viral rna, suggesting that the majority of sirnas are produced through the direct cleavage of the viru ...200616815561
selenium speciation from food source to metabolites: a critical review.especially in the last decade, a vast number of papers on se and its role in health issues have been published. this review gives a brief, critical overview of the main analytical findings reported in these papers. of particular interest is the se content in different food sources worldwide and the extent to which their consumption is reflected in the se content of human tissues and body fluids. several food sources, both natural (brazil nuts, garlic, brassica juncea) and se-enriched (yeast-base ...200616830114
identification of immunodominant regions of brassica juncea glyoxalase i as potential antitumor immunomodulation targets.glyoxalase i activity has been shown to be directly related to cancer and its inhibitors have been used as anti-cancer drugs. immunochemical studies have shown immunochemical relatedness among animal and plant glyoxalase i, but its potential application for biomedical research has not been investigated. in order to understand the conserved immunochemical regions of the protein and to determine probable immunomodulation targets, a cellulose-bound scanning peptide library for brassica juncea glyox ...200515652645
new sesquiterpenic phytotoxins establish unprecedented relationship between different groups of blackleg fungal isolates.a comprehensive search for sesquiterpenic metabolites produced by isolates of the blackleg fungus [leptosphaeria maculans (desm.)] ces. et de not. [asexual stage phoma lingam (tode ex fr.) desm.] revealed that an isolate pathogenic on both canola and brown mustard (ibcn 18) and two isolates pathogenic on brown mustard (laird 2 and mayfair 2) produced similar sesquiterpenes. the isolation, chemical structure elucidation, and phytotoxicity of these new sesquiterpenes with silphinene and selinene t ...200515755649
brassica juncea hmg-coa synthase: localization of mrna and protein.3-hydroxy-3-methylglutaryl-coenzyme-a (hmg-coa) synthase (hmgs; ec synthesizes hmg-coa, a substrate for mevalonate biosynthesis in the isoprenoid pathway. it catalyzes the condensation of acetyl-coa with acetoacetyl-coa (acac-coa) to yield s-hmg-coa and hs-coa. in brassica juncea (indian mustard), hmgs is encoded by four isogenes (bjhmgs1-bjhmgs4). we have already enzymatically characterized recombinant bjhmgs1 expressed in escherichia coli, and have identified its residues that are si ...200515770484
indian mustard [brassica juncea (l.) czern.].all economically important brassica species have been successfully transformed using agrobacterium tumefaciens. although different tissues have been used as explants, hypocotyls remain the most desirable explants for brassica tissue culture owing to their amenability to regeneration. young explants excised from 3- to 4-d-old seedlings have exhibited optimal regeneration potential; the addition of adjuvants such as silver nitrate to the selection medium is necessary to achieve high efficiency of ...200616988352
rga- and rapd-derived scar markers for a brassica b-genome introgression conferring resistance to blackleg in oilseed introgression derived from the b genome of brassica juncea in spring-type oilseed rape (b. napus) conferring recessively inherited cotyledon resistance against several pathotypes of the blackleg fungus leptosphaeria maculans was mapped using pcr-based molecular markers. resistance-associated b-genome-specific randomly amplified (rapd) and resistance gene analog (rga) dna polymorphisms were converted into three sequence-specific markers (scars; b5-1520, c5-1000, rgalm). the flanking sequence o ...200515887037
uptake of heavy metals and as by brassica juncea grown in a contaminated soil in aznalcóllar (spain): the effect of soil amendments.two crops of brassica juncea (l.) czern. were grown in a field experiment, at the site affected by the toxic spillage of acidic, metal-rich waste in aznalcóllar (seville, spain), to study its metal accumulation and the feasibility of its use for metal phytoextraction. the effects of organic soil amendments (cow manure and mature compost) and lime on biomass production and plant survival were also assessed; plots without organic amendment and without lime were used as controls. plots, with or wit ...200515894412
stepwise engineering to produce high yields of very long-chain polyunsaturated fatty acids in plants.very long chain polyunsaturated fatty acids (vlcpufas) such as arachidonic acid (aa), eicosapentaenoic acid (epa) and docosahexaenoic acid (dha) are valuable commodities that provide important human health benefits. we report the transgenic production of significant amounts of aa and epa in brassica juncea seeds via a stepwise metabolic engineering strategy. using a series of transformations with increasing numbers of transgenes, we demonstrate the incremental production of vlcpufas, achieving a ...200515951804
accumulation of metals and its effects in brassica juncea (l.) czern. (cv. rohini) grown on various amendments of tannery waste.the metal accumulation potential and its tolerance in the plants of brassica juncea (l.) czern. (cv. rohini) grown on various amendments of tannery sludge (10%, 25%, 35%, 50%, 75%, 100%) were studied after 30, 60, and 90 days after sowing. these plants were found to be effective accumulators of metals (cr, fe, zn, and mn); however, the seeds accumulated the least quantities of all the metals tested. the oil content of the seeds was found to increase up to 35% tannery sludge followed by a decreas ...200515978297
influence of plant growth promoting bacteria and cr6+ on the growth of indian mustard.the cr(6+) resistant plant growth promoting bacteria (pgpb), pseudomonas sp. psa4 and bacillus sp. ba32 were isolated from heavy metal contaminated soils and their plant growth promoting activity on the indian mustard (brassica juncea) were assessed with different concentrations of cr(6+) in soil. production of siderophores and the solubilization of phosphate were observed in both strains, psa4 and ba32. production of iaa was only observed in strain psa4. inoculation of psa4 or ba32 promoted the ...200615982703
putrescine alleviation of growth in salt stressed brassica juncea by inducing antioxidative defense system.seedlings of indian mustard (brassica juncea rh-30) grown in controlled condition (irradiance 75 wm(-2), rh 60-70% and temp. 25 +/- 2 degrees c) for 7d and watered with hoagland's solution containing different level of nacl (50-250 mmol/l nacl) with or without putrescine (put, 0.1 mmol/l) were examined for put amelioration of nacl induced inhibition in seedling growth by altering activity of antioxygenic enzymes and level of free radicals in the leaves. salinity caused reduction in seedlin ...200516008089
identification of new isolates of turnip mosaic virus that cluster with less common viral strains.turnip mosaic virus (tumv) was found infecting cultivated brassicas and wild and cultivated ornamental brassicaceae plants in different regions of spain. five new tumv isolates, originating from different host plant species (brassica cretica, brassica juncea, brassica napus, eruca vesicaria subsp. sativa and sisymbrium orientale), have been identified. the nucleotide sequences of the coat protein (cp) genes of the five isolates were determined. phylogenetic analysis of the cp sequences showed th ...200717347771
saskatchewan. suspected oriental mustard seed (brassica juncea) poisoning in cattle. 198917423378
biodegradation of glucosinolates in brown mustard seed meal (brassica juncea) by aspergillus sp. nr-4201 in liquid and solid-state cultures.aspergillus sp. nr-4201 was assessed by degrading glucosinolates in brown mustard seed meal (brassica juncea). a liquid culture of the strain, in a medium derived from the meal, produced total degradation of glucosinolates at 32 h. under these conditions, the glucosinolate-breakdown product, allylcyanide, was formed in culture filtrates. in a plate culture under sterile conditions, the growth of the strain in heat-treated meal media was shown to be effective at 30 degrees c with 51% moisture, as ...200212713131
phytochelatin synthase (pcs) protein is induced in brassica juncea leaves after prolonged cd exposure.higher plants respond to cadmium exposure with the production of phytochelatins (pcn), small heavy metal binding peptides, which are synthesized from glutathione by phytochelatin synthase (pcs). the isolation of a pcs cdna clone from brassica juncea l. cv. vitasso, a candidate species for phytoremediation, is reported here. clustal analysis revealed a close relationship of bjpcs1 with pcs proteins from arabidopsis thaliana and thlaspi caerulescens. bjpcs1 expressed as recombinant protein in e. c ...200312815036
extraction and determination of glucosinolates from soil.the use of glucosinolate-containing plants as soil-incorporated biofumigants for pest and disease control has raised questions regarding the fate of glucosinolates in soil; however, no method for routine analysis of glucosinolates in soil has been reported. a simple method to extract glucosinolates from soil with quantification as desulfoglucosinolates by hplc is presented. the method involves two extractions with 70% methanol at room temperature, centrifugation, and filtration prior to the desu ...200516332113
improved understanding of hyperaccumulation yields commercial phytoextraction and phytomining technologies.this paper reviews progress in phytoextraction of soil elements and illustrates the key role of hyperaccumulator plant species in useful phytoextraction technologies. much research has focused on elements which are not practically phytoextracted (pb); on addition of chelating agents which cause unacceptable contaminant leaching and are cost prohibitive; and on plant species which offer no useful phytoextraction capability (e.g., brassica juncea czern). nickel phytoextraction by alyssum hyperaccu ...200717766822
mapping genes for resistance to leptosphaeria maculans in brassica juncea.blackleg disease of crucifers, caused by the fungus leptosphaeria maculans, is a major concern to oilseed rape producers worldwide. brassica species containing the b genome have high levels of resistance to blackleg. brassica juncea f2 and first-backcross (b1) populations segregating for resistance to a pg2 isolate of l. maculans were created. segregation for resistance to l. maculans in these populations suggested that resistance was controlled by two independent genes, one dominant and one rec ...200616462899
persistence of acetamiprid in/on mustard (brassica juncea l.). 200616468018
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