hepatic nucleases. extrahepatic origin and association of neutral liver ribonuclease with the large granule fraction of rat liver, the density distribution of inhibitor-sensitive neutral ribonuclease is similar to that for acid hydrolases and its density distribution is similarly modified by triton wr-1339 accumulation in lysosomes. particulate neutral ribonuclease is latent; the enzyme is unmasked by very low digitonin concentrations or hypoosmotic shock. these observations demonstrate that the bulk of liver neutral ribonuclease is associated with the lysosomal system. in view of ...19751273
effect of cyanide on nadph oxidation by granules from human polymorphonuclear leukocytes.cyanide has been shown to stimulate both oxygen uptake and hexose monophosphate shunt activity in phagocytizing human polymorphonuclear leukocytes. it also stimulates the oxidation of nadph by a particulate fraction derived from phagocytizing cells. this stimulation of nadph oxidase is not observed in the presence of exogenous mn2+. studies with purified enzymes have shown that cn- also stimulates nadph oxidation by horseradish peroxidase or lactoperoxidase, suggesting that the respiratory burst ...197713879
purification of bovine thyroid peroxidase.trypsin-solubilized peroxidase activity from beef subcellular particles was resolved by deae-cellulose chromatography into 5 fractions, which contained enzymatically active components that ranged in molecular size from 73,000 to 340,000 daltons. the most active fraction (mol wt, 92,000 by gel filtration) was further purified (59,000-fold overall) by chromatography on hydroxylapatite. this highly purified peroxidase preparation had an absorbance purity ratio (a410:a280) of 0.55 and oxidized iodid ...197715822
the role of cytochrome p-450 in the hydroperoxide-catalyzed oxidation of alcohols by rat-liver microsomes.the organic hydroperoxide cumene hydroperoxide is capable of oxidizing ethanol to acetaldehyde in the presence of either catalase, purified cytochrome p-450 or rat liver microsomes. other hemoproteins like horseradish peroxidase, cytochrome c or hemoglobin were ineffective. in addition to ethanol, higher alcohols like 1-propanol, 1-butanol and 1-pentanol are also oxidized to their corresponding aldehydes to a lesser extent. other organic hydroxyperoxides will replace cumene hydroperoxide in oxid ...197720305
adsorption of horseradish peroxidase, ovomucoid and anti-immunoglobulin to colloidal gold for the indirect detection of concanavalin a, wheat germ agglutinin and goat anti-human immunoglobulin g on cell surfaces at the electron microscopic level: a new method, theory and application.a method is described for the adsorption of selected macromolecules to colloidal gold which is then used as an electron dense marker for the indirect detection of specific cell surface molecules. membrane bound concanavalin a, which binds specific sugars on horseradish peroxidase, and wheat germ agglutinin, which binds specific sugars on ovomucoid are detected indirectly with gold labeled horseradish peroxidase and ovomucoid, respectively. goat anti-human igm on blood lymphocytes is detected wit ...197721217
tetramethyl benzidine for horseradish peroxidase neurohistochemistry: a non-carcinogenic blue reaction product with superior sensitivity for visualizing neural afferents and efferents.tetramethyl benzidine (tmb) is a presumptively non-carcinogenic chromogen which yields a blue reaction-product at sites of horseradish peroxidase activity. sixty-six distinct procedures were performed in rats and monkeys in order to determine the optimal incubation parameters for tmb. as a result, a procedure is recommended whose sensitivity greatly surpasses that of a previously described benzidine dihydrochloride method. indeed, the sensitivity of this new method in demonstrating retrograde tr ...197824068
effect of beta-lapachone on hydrogen peroxide production in trypanosoma cruzi.beta-lapachone, an antimicrobial agent, markedly increase the generation of h2o2 in intact trypanosoma cruzi epimastigotes (y strain). increase in h2o2 was determined by the horseradish peroxidase-h2o2 compound ii formation as well as by a cytochemical technique.197824995
concanavalin a receptors on the cell membrane of trypanosoma cruzi.epimastigotes and trypomastigotes of t. cruzi obtained from acellular culture as well as bloodstream trypomastigotes agglutinate with concanavalin a (con a). con a-binding sites were also localized on the cell membrane by using the con a-horseradish peroxidase-diaminobenzidine method. passage of epimastigotes and trypomastigotes from acellular culture through deae-cellulose column did not affect con a-binding sites as detected by agglutination and electron miscroscopy.197828652
reaction of horseradish peroxidase with azide and some implications for the heme environmental structure. nmr and kinetic studies.the azide complex of horseradish peroxidase was studied by high resolution 1h and 15n nmr spectroscopy and by the temperature-jump method. the heme peripheral methyl proton peaks and the ligand 15n resonance were resolved to show that binding of azide by horseradish peroxidase occurs only in acidic solution below ph 6.5. it was also found that the chemical exchange rate of azide with the ferric enzyme was much faster on the 1h and 15n nmr time scale. this was further substantiated by kinetics of ...197831922
immunocytochemical localization of neuronal antigens: tyrosine hydroxylase, substance p, [met5]-enkephalin.neuronal antigens can be demonstrated histologically by numerous direct and indirect immunocytochemical techniques in which a specific antibody is identified by a marker compound such as fluorescein isothiocyanate, ferritin, or horseradish peroxidase. one of the more sensitive methods for the light and electron microscopic localizations of antigens in sections of tissue is the peroxidase-antiperoxidase (pap) technique. the experimental procedures and the results obtained using this technique for ...197939006
postnatal rat sympathetic neurons in culture. i. a comparison with embryonic neurons.1. a morphological and physiological comparison was made between embryonically and postnatally derived superior cervical ganglion neurons (scgn) grown in dissociated cell culture. it was found that while morphologically distinct, the physiological properties of the postnatal neurons were the same as their embryonic counterparts. 2. intracellular injection of horseradish peroxidase (hpr) demonstrated that scgn from any age of animal elaborated two basic types of processes, although the pattern of ...197939983
distribution of immune deposits in the skin of reversed passive arthus reaction using horseradish peroxidase as antigen. 197645717
junctional complexes in the preimplantation rabbit embryo.the morphology and development of junctional complexes between blastomeres of the preimplantation rabbit embryo were investigated using several approaches. electron microscopic examination of embryos stained en bloc with uranyl acetate, and the study of junction permeability using horseradish peroxidase and lanthanum nitrate provided information on structure, intermembrane spacing and permeability of the junctional complexes. in addition, the freeze fracture technique was used with day 5 and day ...197545878
localization of immunoreactive androgen in testicular tissue.immunohistochemical localization of androgen in the testes of both rat and squirrel monkey has been performed using an antiserum specific to testosterone and certain related androgens. the bound antibodies were visualized by coupling with antigammaglobulin labeled either with fluorescein isothiocyanate or with a horseradish peroxidase-diaminobenzidine technique. the highest concentration of androgen was found in the interstitial tissue but a significant amount was also present in the seminiferou ...197545910
thalamic afferents to areas 17, 18 and 19 of cat cortex traced with horseradish peroxidase. 197546157
retrograde axonal transport of horseradish peroxidase in sensory nerves and ganglion cells of the rat. 197546159
retrograde intraaxonal transport of horseradish peroxidase in retinal ganglion cells of the chick. 197546164
the orthograde transport of horseradish peroxidase in the visual system. 197546168
a comparison of anterograde and retrograde axonal transport of horseradish peroxidase in the connections of the mammillary nuclei in the rat. 197546171
electron microscopic observations of horseradish peroxidase transported from the caudoputamen to the substantia nigra in the rat: possible involvement of the agranular reticulum.the intracellular distribution of horeseradish peroxidase (hrp) transported intraaxonally from the caudoputaminal complex to the substantia nigra has been examined with the electron microscope. the reciprocal axonal connections between the caudoputamen and the substantia nigra permitted observation not only of hrp transported retrogradely from axons and axon terminals in the caudoputamen to the cell bodies of origin in the pars compacta of the substantia nigra, but also provided information sugg ...197546174
corticothalamic neurons and thalamocortical terminal fields: an investigation in rat using horseradish peroxidase and autoradiography.subsequent to thalamic injections in rats of horseradish peroxidase (hrp) alone or hrp and [3h]leucine in combination, the cells of origin of the corticothalamic projections and the terminal fields of the thalamocortical projections were identified. hrp-labeled corticothalamic neurons were uniformly found in layers v and vi. they were medium to small in size and always pyramidal in shape with the larger neurons being found in layer v. on the other hand, 3 different patterns for the distribution ...197546175
use of peroxidatic-enzyme staining to enhance resolution of cultured mammalian cells under phase microscopy.staining of glutaraldehyde-fixed mammalian cells with peroxidatic enzymes (horseradish peroxidase or horse heart cytochrome c) greatly enhances resolution of their structure under phase microscopy. the topography of cell processes and regions of intercellular contact and overlapping is resolved precisely, even in dense cultures mounted in media which ordinarily do not permit clear demonstration of these areas. the technique is therefore a useful aid to the study of cultured cells with phase opti ...197546631
genetic control of sensitization to structurally unrelated antigens and its relationship to histocompatibility antigens in guinea-pigs.five strains of guinea-pigs homozygous for gpl-a and 2/13 histocompatibility antigens were sequentially immunized with seven structurally unrelated antigens (tgal, bpo-bgg, asan, bpo-pll, phenetidine, horseradish peroxidase and dnp-dodecapeptide). considerable differences in the immune response were observed, as measured by delayed skin reactivity and antigen-induced lymphocyte stimulation in vitro. the data presented show that when the progeny of various back-cross matings are analysed for thei ...197547308
olivocochlear and vestibular efferent neurons of the feline brain stem: their location, morphology and number determined by retrograde axonal transport and acetylcholinesterase histochemistry.anterograde degeneration studies have shown that the cochlear and vestibular receptor organs receive an efferent innervation from neurons in the brain stem. this pathway may provide a mechanism by which the cns could modulate its own afferent input. the neurons which provide this innervation have so far escaped positive identification with methods which depend on retrograde cell changes after axotomy. in the present study, horseradish peroxidase (hrp) was injected into the labryinths of kittens ...197547866
a comparative study of horseradish peroxidase conjugates prepared with a one-step and a two-step this study we compared horseradish peroxidase (hrp)-labeled rabbit antihuman immunoglobulin g (igg) conjugates, prepared by a one-step and a two-step method. glutaraldehyde was used as a cross-linking agent. two methods were used for removing unconjugated hrp: sephadex g-200 gel chromatography and ammonium sulfate precipitation. the conjugates were characterized immunologically, immunochemically and enzymatically. the immunohistoenzymic properties of the conjugates were tested on unfixed cryo ...197547869
the retrograde transport method.after an injection of horseradish peroxidase into the brain, the enzyme product can later be found in cell bodies of neurons which project axons to the injection site. the use of the tracer has now been adapted as a means of identifying neurons on the basis of their efferent fiber projections. in this review particular attention is paid to characteristics of the phenomenon of retrograde transport, such as diffusion of the marker from the injection site and the region of effective uptake, uptake ...197548482
retrograde axonal transport of horseradish peroxidase in peripheral autonomic exogeneous marker protein, horseradish peroxidase (hrp) was used to race peripheral autonomic pathways in adult guinea pigs and cats. small doses of hrp were injected into various organs and after a brief survival period, hrp activity appeared in the perikarya of autonomic neurons that supplied each injection site. after injection of hrp into the anterior chamber of the eye, reaction product was detected in the postganglionic sympathetic neurons of the superior cervical sympathetic ganglion. ...197548519
retrograde transport of horseradish peroxidase in the magnocellular neurosecretory system of the rat.horseradish peroxidase (hrp) was injected into the pituitary in adult rats. after 2-3 days, the neurones of the supraoptic nuclei, the magnocellular parts of the paraventricular nuclei, and the various accessory neurosecretory hypothalamic nuclei showed accumulation of hrp. the hrp reaction product consisted of fine, discrete cytoplasmic granules, and in electron micrographys it was seen to be located in the lysosome-like dense bodies of 0.4-0.6 mum diameter which are normally present in the cyt ...197549208
the origin of fornix fibers which project to the mammillary bodies in the rat: a horseradish peroxidase study. 197549209
demonstration of nigrothalamic connections in the cat by retrograde axonal transport of horseradish peroxidase. 197549210
purification of mouse alpha1-fetoprotein and preparation of specific peroxidase conjugates for its cellular localization.mouse alpha1-fetoprotein (afp) was isolated from amniotic fluid by immunoadsorbent columns and preparative electrophoresis. specific antibodies were isolated from monospecific hyperimmunsera by use of immunoadsorbents, and subsequently coupled with horseradish peroxidase. at the light microscopical level, purified antibody-peroxidase conjugates were used for the cellular localization of afp in fetal liver by direct and indirect staining methods. fixatives containing ethanol or aldehydes were tri ...197549343
caveola--vesicle complexes in the plasmalemma of erythrocytes infected by plasmodium vivax and p cynomolgi. unique structures related to schüffner's dots.erythrocytes infected with plasmodium vivax and p cynomolgi, characterized by schüffner's dots on giemsa-stained thin films, were studied by electron microscopy and immunocytochemistry. a caveola-vesicle complex, which consisted of a caveola surrounded by vesicles, in an alveolar fashion, formed along the erythrocyte plasmalemma. horseradish-peroxidase-labeled immunoglobulin from a monkey infected with p vivax bound to the vesicle membrane. cationized ferritin appeared within the vesicles after ...197550017
projections to the gyrus proreus from the brain stem tegmentum (locus coeruleus, raphe nuclei) in the cat, demonstrated by retrograde transport of horseradish peroxidase. 197550117
[uptake and transport of exogenous peroxidase in the masticatory muscles of cats. localization of ganglionic neurons].retrograde axonal transport of horseradish peroxydase (hrp) has been used to trace the cells of origin of proprioceptive fibers in jaw-closing and jaw-opening muscles. after injection of hrp in young cats' masticatory muscles (masseteric, temporal, pterygoid, mylohyoid and digastric) labelled neurons were found in the ipsilateral semi-lunar ganglion and trigeminal mesencephalic nucleus. it is concluded that sensory endings are present in jaw-opening as in jaw-closing muscles; possibly the affere ...197550149
[uptake and transport of exogenous peroxidase in the masticatory muscles of the cat. localization of motor neurons].in the present work, a topographical localization of masticatory muscle motoneurons was undertaken. horseradish peroxydase injected in each muscle can be transported in the retrograde direction to the corresponding motoneurons cell bodies. jaw-closing muscle motoneurons were identified in the dorsal part of the motor trigeminal nucleus whereas jaw-opening muscle motoneurons were observed in the ventro-medial region.197550152
studies on the cerebellar projections from the main and external cuneate nuclei in the cat by means of retrograde axonal transport of horseradish peroxidase.the cerebellar projections from the main and external cuneate nuclei in the cat have been studied by means of retrograde axonal transport of horseradish peroxidase. the main projection from the external cuneate nucleus (ecn) is to the intermediate and, possibly, the small lateral part of lobule v and to the paramedian lobule on the ipsilateral side. the projection from the ecn to the cerebellar regions mentioned is topographically organized. cells in the caudal part of the ecn send their axons t ...197550867
ultrastructural localization of gamma-chain and immunoglobulin g in human lymphocytes using enzyme-labeled fab the use of rabbit antibodies against the heavy chain of human immunoglobulin g (igg), the gamma-chain and igg molecules were successfully localized at the ultrastructural level in human peripheral lymphocytes. the rabbit fab fragment was coupled to horseradish peroxidase by means of glutaraldehyde and the resulting conjugate could penetrate the intact plasma membrane. discernible reaction product was observed in cisternae of the nuclear envelope, rough endoplasmic reticulum and golgi apparatu ...197551039
the identification of striatal and pallidal neurons projecting to substantia nigra. an experimental study by means of retrograde axonal transport of horseradish peroxidase. 197551667
a study of the dynamics of retrograde transport and accumulation of horseradish peroxidase in injured neurons.the phenomenon of retrograde intraaxonal transport of extracellular markers introduced at the level of the axon terminal has been suggested as a possible mechanism of communication between the axon terminal and the neuron cell body. we tested the hypothesis that communication after axotomy might consist of a change in the rate of uptake or of transport of material by injured neurons. small lesions were made with a needle in one retinal quadrant of chicks and immediately afterwards horseradish pe ...197553088
preganglionic innervation of the adrenal gland of the rat: a study using horseradish peroxidase. 197553160
retrograde transport of horseradish peroxidase in transected axons. ii. relations between rate of transfer from the site of injury to the perikaryon and onset of chromatolysis.the sciatic nerves of 2--3 month-old mice, rats and rabbits were crushed and at the same time horseradish peroxidase (hrp) was applied around the crushed region at mid-thigh level. at varying time intervals thereafter, hrp was seen in neurons of the corresponding spinal ganglia and levels of the spinal cord. the rate of ascent of hrp appeared to be similar in the different animals and to be independent of the length of the nerve. the accumulation of hrp was more conspicuous in small than large n ...197553277
localization of laryngeal motor neurons in the a series of 12 newborn kittens, horseradish peroxidase (hrp) was used to trace retrograde axoplasmic flow in the motor neurons to laryngeal muscles. the animals were sacrificed 24 hours after injection of hrp into a specific laryngeal muscle, and the brain stems were stained for peroxidase. this clear-cut colorimetric method permitted the localization of the motor neurons in two nuclei of the ipsilateral brain stem. these are the nucleus ambiguus and the retrofacial nucleus. the primary sourc ...197553770
electron microscopic demonstration of immunoglobulin deposition in rheumatoid cartilage.horseradish peroxidase (hrpo) conjugated with goat antihuman igg, goat antihuman igm, and aggregated human igg has been used as a enzymatic marker to stain igg, igm, and rheumatoid factor in rheumatoid cartilage. when hrpo-anti igg and hrpo-anti igm were used, immunoglobulin deposits were not observed in nonrheumatoid cartilage. however 7 of 8 rheumatoid cartilage specimens stained with hrpo-anti igg showed electron-dense deposits. three rheumatoid specimens stained with hrpo-anti igm showed sim ...197554168
rôle of axonal transport in maintaining central synaptic connections.synaptic boutons on the somata of rat hypoglossal neurones have been stained with zinc iodide-osmium, and counted using light microscopy. two weeks after silastic cuffs containing colchicine or vinblastine were applied to the hypoglossal nerve, the number of boutons on parent nerve cell bodies was significantly lower than controls. these drugs inhibited retrograde axonal transport of horseradish peroxidase, but did not affect neuromuscular conduction or the number of axons in the nerve trunk. cu ...197554262
prenatal development of central optic pathways in albino rats.the development of the central optic projections in albino rat fetuses has been studied using light and electron microscopic degeneration techniques and the horseradish peroxidase method for demonstrating axonal projections of neurons. the first optic axons to reach the region of the optic chiasm arrive at day 15. by day 16, a substantial optic chiasm is seen and the optic tract can be traced into the epithalamus, having first passed through the ventral lateral geniculate nucleus and a thin lami ...197654370
retrograde transpost of horseradish peroxidase in afferent and efferent neurons from masseter muscle in the rat. 197554884
tracing axons and axon collaterals of spinal neurons using intracellular injection of horseradish peroxidase.intracellular injection and subsequent histochemical localization of horseradish peroxidase have been used to stain the soma, dendrites, axons, and axon collaterals of spinalcervical tract neurons and unidentified dorsal horn neurons in the cat. this technique may be used in combination with the intracellular injection of procion yellow to demonstrate by light microscopy connections between physiologically typed vertebrate neurons.197654936
factors affecting retrograde axonal transport of horseradish peroxidase in the visual system. 197655291
light and electron microscopic observations on the anterograde transport of horseradish peroxidase in the optic pathway in the mouse and rat. 197655292
electron microscopic immunohistochemical identification of endothelial cells in the rabbit.antibody to tissue factor apoprotein was adsorbed against gamma-globulin and coupled to horseradish peroxidase; this complex was applied to various rabbit tissues. the distribution of the peroxidase marker then was observed by electron microscopy. we examined fixed or frozen sections, as well as breis of aorta, vena cava, brain, heart, lung, liver, spleen, kidney, bone marrow, mesothelial gut lining, erythrocytes, and platelets. all endothelial cells that had been exposed to the antibody complex ...197655315
immunochemical localization of in vivo bound immunoglobulins in pemphigus vulgaris epidermis. employment of a peroxidase-antiperoxidase multistep technique for light and electron microscopy.a multi-step immunocytochemical method utilizing horseradish peroxidase (hrp) as an immunological marker was employed to demonstrate, at the ultrastructural level, igg antiepithelial autoantibodies bound in vivo to pemphigus epidermis, and circulating igg antibodies, using monkey oesophagus as substrate. to demonstrate igg the following antisera were employed in sequential steps: goat antihuman igg; rabbit antigoat igg; goat-anti-hrp-serum. after incubating with the final antigen, hrp, the latte ...197556157
critical factors involved in the demonstration of horseradish peroxidase retrograde transport. 197656209
projection of thalamic neurons to cat primary vestibular cortical fields studied by means of retrograde axonal transport of horseradish peroxidase.the two vestibular cortical projection areas in the anterior suprasylvian sulcus and post-cruciate dimple regions were defined by evoked potential technique in anaesthetized cats. the thalamic location of neurons with axon terminals in these fields was determined using the method of retrograde axonal transport of horseradish peroxidase. the ascending vestibular pathway appeared to be separated also at the thalamic level, where cells in the ventroposterolateral nucleus were found to project to th ...197656281
ultrastructural differences between the preterminal nerve fibres and their endings in the mechanoreceptors, with special reference to their degeneration and mode of uptake of horseradish peroxidase. 197656762
localization of rabbit gamma globulins in the mouse visceral yolk sac placenta.the yolk sac placenta has been implicated previously in transmission of passive immunity to the fetus. this work uses an immunohistochemical technique devised by sternberger et al. ('69) to study this problem. rabbit serum containing gamma globulins was injected into the uterine lumen of the white swiss mouse during the last third of pregnancy. two or four hours later yolk sacs were removed and fixed in 2% paraformaldehyde or freeze-dried and fixed with paraformaldehyde vapors. finely chopped ti ...197656909
a note on the method of retrograde transport of horseradish peroxidase as a tool in studies of afferent cerebellar connections, particularly those from the inferior olive; with comments on the orthograde transport in purkinje cell axons.1. injections of horseradish peroxidase (hrp) suspension were made in the cerebellar cortex of cats (most often the paramedian lobule). the staining of the cerebellar cortex and the ensuing labeling of neurons in the inferior olive were studied in experiments with variations of concentration of hrp, amounts of fluid injected, survival time and age of the animals. light microscopical studies were supplemented with electron microscopical observations. the folded cerebellar cortex offers particular ...197657065
movement of horseradish peroxidase in rabbit submandibular glands after ductal injection.horseradish peroxidase (hrp) has been used as a tracer to study movements of solutions injected retrogradely via the duct of submandibular glands in rabbits. 0.1 ml of solution was injected either manually or by a constant hydrostatic pressure, and the subsequent distribution of hrp in the gland and duct at different times after injection has been examined histochemically at light and electron microscopical levels. shortly after the injections, strong interstitial staining for peroxidase resulte ...197657956
the development of the isthmo-optic tract in the chick, with special reference to the occurrence and correction of developmental errors in the location and connections of isthmo-optic neurons.the development of the centrifugal projection to the chick retina in the isthmo-optic tract (iot) has been studied by the retrograde transport of the enzyme marker horseradish peroxidase (hrp) injected into the eye at various times during the incubation period. some neurons in the isthmo-optic nucleus (ion--the cells of origin of the iot) can first be labeled from the eye following injections on the tenth day of incubation; after injections on the twelfth day or later, about 95% of the neurons c ...197658875
a guide to the synaptic analysis of the neuropil.a morphological analysis of the organization of the gray matter in the central nervous system depends on the discovery of consistent repetitive patterns. without these, the gray matter remains a chaotic jungle. an hypothesis derived from the study of a few simple regions has been developed to serve as a guide in finding these patterns. it states that all nerve fibers and terminals arising from a particular group of nerve cells, or, more precisely, a particular nerve cell type, display similar ax ...197659655
anti-streptococcal antibodies reacting with brain tissue. ii. utrastructural studies.the reaction of rabbit anti-streptococcus pyogenes type 24 with mouse brain has been studied at the ultrastructural level using horseradish peroxidase labelled antiglobulin. it is shown that these antibodies, and by inference those found in sydenham's chorea, react with the fibrous astrocyte. the cross-reacting antigen is the glial filament or a closely associated cytoplasmic constituent.197660120
differences between alpha and gamma motoneurons labeled with horseradish peroxidase by retrograde transport. 197660166
immune complex receptors on cell surface. i. ultrastructural demonstration of macrophages.a method is described for ultrastructural localization of immune complex receptors on the surface of viable peritoneal exudate cells. the technique entails incubation with a soluble complex of horseradish peroxidase (hrp) and specific antibody to hrp at 4 degrees c followed by exposure to diaminobenzidine and processing for electron microscopy. the bound immune complexes were evident as focal deposits of hrp reaction product, adhering closely to the external surface of macrophages with an uninte ...197660441
the olivocerebellar projection in the cat studied with the method of retrograde axonal transport of horseradish peroxidase. iii. the projection to the vermal visual area.horseradish peroxidase (hrp) was injected separately in one of the cerebellar lobules vi, viia, viib, viiia or viiib (together corresponding to the vermal visual area) in 17 cats. after 1-3 days the distribution of labeled cells in the inferior olive was mapped. in spite of some overlapping it is clear that the various lobules of the vermal visual area receive fibers from separate parts of a horseshoeshaped region in the caudal half of the contralateral medial accessory olive (fig. 5c). the proj ...197661211
comparative immunoenzymatic localization of prolactin and growth hormone in human and rat pituitaries.twelve human and twelve rat pituitaries were stained by an immunohistochemical method using a rabbit anti-ovine prolactin serum, a rabbit anti-human growth hormone serum and a sheep anti-rabbit immunoglobulin serum conjugated with horseradish peroxidase. on the same pituitary section, growth hormone cells were stained brown by using 3-3'-diaminobenzidine as peroxidase substrate, and prolactin cells were stained purplish blue by using 4-chloro-1-naphtol. growth hormone cells outnumbered prolactin ...197661240
movement of horseradish peroxidase in the anterior parts of the eye [proceedings]. 197561704
retrograde transport of horseradish peroxidase in transected axons. 3. entry into injured axons and subsequent localization in perikaryon.horseradish peroxidase (hrp) applied to crushed mouse sciatic nerves diffused through the damaged perineurium into the endoneurium. in the injured area, hrp passed into damaged myelinated and unmyelinated axons forming columns of reaction product, which extended for several millimeters proximally to the lesion. ultrastructurally, hrp adhered to the inner surface of the axoplasm and to the surfaces of neurotubules and neurofilaments in such columns. at more proximal levels axons contained hrp in ...197661782
afferents to the rat substantia nigra studied with horseradish peroxidase, with special reference to fibres from the subthalamic nucleus. 197661785
a horseradish peroxidase study of the cortico-olivary projection in the cat. 197661790
direct projections from the mesodiencephalic midline areas to the pericruciate cortex in the cat: an experimental study with the horseradish peroxidase method. 197661792
nigrothalamic projections in the rat as demonstrated by orthograde and retrograde tracing echniques.the nigrothalamic projection of the rat was investigated by means of horseradish peroxidase (hrp) histochemistry and by the autoradiographic tracing technique. the use of these techniques has permitted an extension of previous descriptions by identifying a neuron population in the substantia nigra, pars reticulata (snr) as the nigrothalamic cells of origin. these calls (20-25 mu) were multipolar and were distributed throughout the snr. there was a tendency however for more labelled neurons to be ...197661793
origins of axons in the cat's acoustic striae determined by injection of horseradish peroxidase into severed and terminations of fibers of the dorsal and intermediate acoustic striae were studied by surgically severing these tracts and injecting hrp into the incision. this procedure results in filling the severed axons with hrp. filled axons were traced to cell groups of origin and to some terminations of the acoustic striae. hrp-labeled terminals were found in the cochlear nuclei as well as in periolivary cell groups. filling of cells with hrp ranged from being complete, resulting in golgi-lik ...197661976
the origin of the pretecto-olivary tract. a study using the horseradish peroxidase method. 197662601
olfactory relationships of the telencephalon and diencephalon in the rabbit. iii. the ipsilateral centrifugal fibers to the olfactory bulbar and retrobulbar formations.the axoplasmic retrograde transport of horseradish peroxidase (hrp) from axon terminals to their parent cell bodies and histochemical fluorescence microscopy have been used to study the ipsilateral centrifugal fibers to the olfactory bulbs and anterior olfactory nucleus in the rabbit. focal injections of peroxidase were placed unilaterally into the main or accessory olfactory bulb or into the anterior olfactory nucleus. in animals with injected hrp confined within the main bulb, perikarya retrog ...197662770
the use of glutaraldehyde-conjugated horseradish peroxidase-bovine serum albumin in the visualization of concanavalin a binding to tissue sections of human colonic tumor.a method is reported for the preparation of glutaraldehyde cross-linked horseradish peroxidase conjugates for the visualization of concanavalin a binding to frozen sections. the binding of concanavalin a to tissue sections of human colonic tumor is used to compare staining by horseradish peroxidase conjugates and monomers. without decreasing specificity, the use of progressively larger molecular weight conjugates provides progressively greater intensity of staining of the epithelial components o ...197662892
efferent connections of the septal area in the rat: an analysis utilizing retrograde and anterograde transport methods.experiments were performed by either retrograde (horseradish peroxidase histochemistry) or anterograde ([3h]leucine radioautography) transport methods to determine the efferent connections of the septum in the rat. it was observed that the dorsal septum projects to the lateral preoptic area, lateral hypothalamus, periventricular hypothalamus and midline thalamus. fibers from the ventral half of the septum project topographically to the hippocampal formation, thalamus, hypothalamus and midbrain. ...197763306
the climbing fibers of the cerebellar cortex, their origin and pathways in cat.the sources and pathways of the climbing fibers to the cerebellar posterior vermis were studied with comibined electrophysiological and anatomical methods in cats. recording from identified cerebellar purkinje cells, monosynaptic climbing fiber (cf) responses have been obtained both for stimulation of the inferior olive (io) and various parts of the brain stem (bs). cf responses were found to of three types, io only, bs only or both io and bs. however the responses to bs stimulation were very fe ...197663384
horseradish peroxidase: an improvement in intracellular staining of single, electrophysiologically characterized neurons. 197663386
the anatomical organization of the cerebello-olivary projection in the cat.the cerebello-olivary pathway in the cat has been examined using orthograde and retrograde neuroanatomical tracing techniques. the orthograde transport of 3h-leucine from injection sites in the deep cerebellar nuclei labeled dentate and interpositus projections to the rostral two-thirds of the contralateral inferior olivary complex. these projections are topographically organized, with the dentate nucleus projecting to the principal olivary nucleus and the posterior and anterior interpositus nuc ...197663472
the blue reaction product in horseradish peroxidase neurohistochemistry: incubation parameters and visibility.a blue reaction product is formed at sites that contain horseradish peroxidase (hrp) activity when benzidene is used as the chromogen. with neutral red as a counter stain, this method affords excellent visualization of both retrograde and orthograde axonal transport of intracerebrally injected hrp. the visibility of this blue reaction-product is better than the visibility of the brown reaction-product obtained in the commonly used diaminobenzidene procedures. variations in incubation times and r ...197663512
intraaxonal transport of horseradish peroxidase in the sympathetic nervous system.following a single injection of horseradish peroxidase (hrp) into the superior cervical ganglion (scg) of the rabbit, the uptake and anterograde transport of this label was confirmed in the ganglion cell bodies, postganglionic axons, and preterminal and terminal ending axons in the ciliary processes of the eye. from the same injection site the intraaxonal hrp reaction product was demonstrated in myelinated axons, presumably by retrograde transport. intracytoplasmic hrp was identified in large, s ...197764283
identification of cells or origin of non-primary afferents to the dorsal column nuclei of the order to identify cells of origin in the spinal cord of non-primary afferents to the dorsal column nuclei (dcn), the retrograde transport of horseradish peroxidase (hrp) has been utilized in adult cats. 10 to 30% hrp was injected bilaterally (0.6 mul per side) in the dorsal medulla of nine cats. in most instances the spread of the injected enzyme extended a few millimeters rostrocaudally and infiltrated the dcn as well as other nuclei and fiber tracts. labelled cells in these cases are numero ...197764365
anatomical studies of a temporal visual area in the rabbit.using fink-heimer, autoradiographic and horseradish peroxidase techniques, the connections of a temporal visual cortical region of the rabbit were explored. the temporal visual area covers portions of areas t1 and t2, and is reciprocally connected with the posterior nucleus and suprageniculate nuclei of the thalamus. it was also shown that the temporal visual area projects to a similar region in the opposite hemisphere, and to intermediate laminae of the superior colliculus. the temporal visual ...197764476
functional organization of the vestibular input to the anterior and posterior cerebellar vermis of cat.1. responses evoked by electrical stimulation (auditory division of the viiith nerve sectioned chronically) and natural stimulation of the vestibular apparatus were recorded in the anterior and posterior cerebellar vermis of cats anesthetized with ketamine or nembutal. under ketamine the functional state of the cerebellar cortex was similar to that of the decerebrate or encéphale isolé preparation. 2. vestibular-evoked responses were found bilaterally throughout the vermis (lob. i-x) and parts o ...197765291
the olivocerebellar projection in the cat studied with the method of retrograde axonal transport of horseradish peroxidase. iv. the projection to the anterior lobe.following injections of horseradish peroxidase (hrp) in the cerebellar cortex of the anterior lobe of the cat, the distribution of labeled cells in the inferior olive was mapped. the findings largely confirm those made previously in studies of olivary retrograde cell loss following cerebellar ablations (brodal, '40b). in addition, they reveal further olivary areas projecting onto the anterior lobe, and permit a more detailed analysis of the pattern in this projection. concerning major points the ...197765365
brain stem afferents to the fastigial nucleus in the cat demonstrated by transport of horseradish peroxidase.although retrograde and anterograde degeneration studies have provided important information concerning brain stem afferents to the fastigal nucleus (fn), these data may be incomplete and should be confirmed by axonal transport methods. attempts were made to inject horseradish peroxidase (hrp) unilaterally into the fn in a series of adult cats. animals were perfused with dextran and a fixative solution of paraformaldehyde and glutaraldehyde in 0.1 m phospate buffer. representative sections were ...197765366
the vestibular complex of the american opossum didelphis virginiana. ii. afferent and efferent connections.we have demonstrated the connectivity of the opossum's vestibular nuclei using degeneration, autoradiographic and horseradish peroxidase techniques and have found it to be generally comparable to that reported for the cat. apart from the primary input described in part i of our study, the cerebellum provides the major source of afferent connections to the vestibular complex. axons from the cerebellar cortex distribute mainly to vestibular areas which receive no primary afferent projections, e.g. ...197765367
ultrastructural visualization of cellular carbohydrate components by means of lectins on ultrathin glycol methacrylate sections.a method for the visualization of cellular carbohydrate components by both light and electron microscopy using lectins on glycol methacrylate sections is proposed. this method, which is an application of the lectin-peroxidase affinity technique, solves the problem of limited penetration when it is attempted to demonstrated lectins receptors within the tissue block. following partial dissolution of glycol methacrylate from thin sections using alcohol, they are incubated successively with lectin ( ...197765417
organization of the facial nerve nucleus in the cat.the location of efferent neurons in the brain stem of newborn kittens supplying the facial muscles was studied by means of horseradish peroxidase (hrp) used as a tracer of retrograde protein transport. three to 15 microliters containing 0.3 to 1.5 mg of hrp was injected into the orbicularis oris and orbicularis oculi muscles of six kittens and in the seventh kitten, the ventral ramus of the facial nerve was transected and hrp was instilled on the cut end of the nerve. after a survival time of 24 ...197765707
brain stem projections to lobule vii of the posterior vermis in the squirrel monkey: as demonstrated by the retrograde axonal transport of tritiated horseradish peroxidase. 197766085
localization of abductor and adductor fibers in the kitten recurrent laryngeal nerve: use of a variation of the horseradish peroxidase tracer technique. 197766152
retrograde axonal transport of horseradish perioxidase in afferent fibers of the inferior mesenteric ganglion of the guinea pig. identification of the cells of origin in dorsal root ganglia. 197766970
orthograde and retrograde axoplasmic transport during acute ocular hypertension in the monkey.orthograde and retrograde axoplasmic transport have been studied in the optic nerve heads of 37 macaca fascicularis eyes with normal or elevated intraocular pressure (iop) produced by cannulation of the anterior chamber. orthograde transport was labeled by 3h-amino acids injected intravitreally and incorporated into retinal ganglion cell proteins. retrograde transport was studied in the same eyes by injecting horseradish peroxidase (hrp) into one or both optic tracts and dorsal lateral geniculat ...197767096
identification of different size motoneurons labeled by the retrograde axonal transport of horseradish peroxidase.three main groups of motoneurons of different size have been labeled in adult cats by using the method for retrograde axonal transport following injection of horseradish peroxidase in the medial gastrocnemius and soleus muscles. in particular small, medium-size and large neurons which probably correspond respectively to gamma, small alpha and large alpha motoneurons innervating the calf muscles, have been identified and the corresponding area measured.197767590
the organization of thalamocortical relay neurons in the rat ventrobasal complex studied by the retrograde transport of horseradish peroxidase. 197768039
commercial horseradish peroxidase degrades myelin encephalitogenic protein during coupling for immunohistochemical studies.conjugates of myelin encephalitogenic basic protein (ep) and commercial horseradish peroxidase (hrp) have been used for immunohistochemical demonstrations of anti-ep antibody in animals with experimental allergic encephalomyelitis. we performed gel electrophoresis studies on ep-hrp conjugates prepared with glutaraldehyde and on mixtures of ep and hrp incubated without glutaraldehyde. the results show that under conditions of one-and two-step coupling hrp causes rapid loss of the native ep band, ...197768067
[study of the vagal innervation of the cat stomach by means of retrograde axoplasmic transport of horseradish peroxidase--on the localization of the origins of the preganglionic fibers]. 197768782
retrograde axonal transport of horseradish peroxidase. uptake at mouse neuromuscular junctions following systemic injection.eye muscles were examined histochemically for the presence of horseradish peroxidase (hrp) activity after intravenous injections of large doses of this tracer protein. hrp had penetrated through the blood vessels and diffused into the areas of motor end plates, where it outlined axon terminals at the synaptic clefts. hrp was incorporated into pinocytotic vesicles in the axons from where an intraaxonal transport in the retrograde direction to the nerve cell bodies in the brainstem followed. accum ...197769382
demonstration of bilateral claustro-cortical connections in the cat with the method of retrograde axonal transport of horseradish peroxidase.the claustrum of the cat was studied to determine the extent of its projection to the cerebral cortex. neurons in the bilateral dorsal claustrum are labeled by retrograde axonal transport of horseradish peroxidase (hrp) from injection sites of different neocortical areas with preponderance to the ipsilateral side. it is revealed that the dorsal claustrum projects to almost entire regions of the bilateral neocortex and that distribution of labeled neurons in the bilateral claustrum has topographi ...197769423
identification of cells of origin of tectopontine fibers in the cat superior colliculus: an experimental study with the horseradish peroxidase method.the pontine projections from the superior colliculus in the cat have been studied by means of retrograde axonal transport of horseradish peroxidase (hrp). following injections of hrp in the dorsolateral pontine nucleus, where the tectopontine fibers terminate, a fair number of labeled cells are found throughout the rostrocaudal extent of the ipsilateral superior colliculus. relatively few of the labeled cells are of medium size (25-40 micron in diameter), more than 80% are small (10-25 micron), ...197769477
the effects of monocular deprivation on different neuronal classes in the lateral geniculate nucleus of the cat.retrograde axonal transport of horseradish peroxidase (hrp) was used to identify two populations of cells in the lateral geniculate nucleus (lgn) of the cat. hrp was injected into area 17 and 18 separately in the same animals, and the neuronal somata giving rise to thalamo-cortical axons, identified by the presence of granular hrp reaction product within them, were measured. the mean size of lgn neurones labelled by injections in area 17 ("17-relay" cells) was less than of neurones filled from a ...197769546
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