| adsorption of horseradish peroxidase, ovomucoid and anti-immunoglobulin to colloidal gold for the indirect detection of concanavalin a, wheat germ agglutinin and goat anti-human immunoglobulin g on cell surfaces at the electron microscopic level: a new method, theory and application. | a method is described for the adsorption of selected macromolecules to colloidal gold which is then used as an electron dense marker for the indirect detection of specific cell surface molecules. membrane bound concanavalin a, which binds specific sugars on horseradish peroxidase, and wheat germ agglutinin, which binds specific sugars on ovomucoid are detected indirectly with gold labeled horseradish peroxidase and ovomucoid, respectively. goat anti-human igm on blood lymphocytes is detected wit ... | 1977 | 21217 |
| postnatal rat sympathetic neurons in culture. i. a comparison with embryonic neurons. | 1. a morphological and physiological comparison was made between embryonically and postnatally derived superior cervical ganglion neurons (scgn) grown in dissociated cell culture. it was found that while morphologically distinct, the physiological properties of the postnatal neurons were the same as their embryonic counterparts. 2. intracellular injection of horseradish peroxidase (hpr) demonstrated that scgn from any age of animal elaborated two basic types of processes, although the pattern of ... | 1979 | 39983 |
| junctional complexes in the preimplantation rabbit embryo. | the morphology and development of junctional complexes between blastomeres of the preimplantation rabbit embryo were investigated using several approaches. electron microscopic examination of embryos stained en bloc with uranyl acetate, and the study of junction permeability using horseradish peroxidase and lanthanum nitrate provided information on structure, intermembrane spacing and permeability of the junctional complexes. in addition, the freeze fracture technique was used with day 5 and day ... | 1975 | 45878 |
| localization of immunoreactive androgen in testicular tissue. | immunohistochemical localization of androgen in the testes of both rat and squirrel monkey has been performed using an antiserum specific to testosterone and certain related androgens. the bound antibodies were visualized by coupling with antigammaglobulin labeled either with fluorescein isothiocyanate or with a horseradish peroxidase-diaminobenzidine technique. the highest concentration of androgen was found in the interstitial tissue but a significant amount was also present in the seminiferou ... | 1975 | 45910 |
| thalamic afferents to areas 17, 18 and 19 of cat cortex traced with horseradish peroxidase. | | 1975 | 46157 |
| retrograde axonal transport of horseradish peroxidase in sensory nerves and ganglion cells of the rat. | | 1975 | 46159 |
| retrograde intraaxonal transport of horseradish peroxidase in retinal ganglion cells of the chick. | | 1975 | 46164 |
| the orthograde transport of horseradish peroxidase in the visual system. | | 1975 | 46168 |
| a comparison of anterograde and retrograde axonal transport of horseradish peroxidase in the connections of the mammillary nuclei in the rat. | | 1975 | 46171 |
| electron microscopic observations of horseradish peroxidase transported from the caudoputamen to the substantia nigra in the rat: possible involvement of the agranular reticulum. | the intracellular distribution of horeseradish peroxidase (hrp) transported intraaxonally from the caudoputaminal complex to the substantia nigra has been examined with the electron microscope. the reciprocal axonal connections between the caudoputamen and the substantia nigra permitted observation not only of hrp transported retrogradely from axons and axon terminals in the caudoputamen to the cell bodies of origin in the pars compacta of the substantia nigra, but also provided information sugg ... | 1975 | 46174 |
| genetic control of sensitization to structurally unrelated antigens and its relationship to histocompatibility antigens in guinea-pigs. | five strains of guinea-pigs homozygous for gpl-a and 2/13 histocompatibility antigens were sequentially immunized with seven structurally unrelated antigens (tgal, bpo-bgg, asan, bpo-pll, phenetidine, horseradish peroxidase and dnp-dodecapeptide). considerable differences in the immune response were observed, as measured by delayed skin reactivity and antigen-induced lymphocyte stimulation in vitro. the data presented show that when the progeny of various back-cross matings are analysed for thei ... | 1975 | 47308 |
| olivocochlear and vestibular efferent neurons of the feline brain stem: their location, morphology and number determined by retrograde axonal transport and acetylcholinesterase histochemistry. | anterograde degeneration studies have shown that the cochlear and vestibular receptor organs receive an efferent innervation from neurons in the brain stem. this pathway may provide a mechanism by which the cns could modulate its own afferent input. the neurons which provide this innervation have so far escaped positive identification with methods which depend on retrograde cell changes after axotomy. in the present study, horseradish peroxidase (hrp) was injected into the labryinths of kittens ... | 1975 | 47866 |
| a comparative study of horseradish peroxidase conjugates prepared with a one-step and a two-step method. | in this study we compared horseradish peroxidase (hrp)-labeled rabbit antihuman immunoglobulin g (igg) conjugates, prepared by a one-step and a two-step method. glutaraldehyde was used as a cross-linking agent. two methods were used for removing unconjugated hrp: sephadex g-200 gel chromatography and ammonium sulfate precipitation. the conjugates were characterized immunologically, immunochemically and enzymatically. the immunohistoenzymic properties of the conjugates were tested on unfixed cryo ... | 1975 | 47869 |
| retrograde axonal transport of horseradish peroxidase in peripheral autonomic nerves. | an exogeneous marker protein, horseradish peroxidase (hrp) was used to race peripheral autonomic pathways in adult guinea pigs and cats. small doses of hrp were injected into various organs and after a brief survival period, hrp activity appeared in the perikarya of autonomic neurons that supplied each injection site. after injection of hrp into the anterior chamber of the eye, reaction product was detected in the postganglionic sympathetic neurons of the superior cervical sympathetic ganglion. ... | 1975 | 48519 |
| retrograde transport of horseradish peroxidase in the magnocellular neurosecretory system of the rat. | horseradish peroxidase (hrp) was injected into the pituitary in adult rats. after 2-3 days, the neurones of the supraoptic nuclei, the magnocellular parts of the paraventricular nuclei, and the various accessory neurosecretory hypothalamic nuclei showed accumulation of hrp. the hrp reaction product consisted of fine, discrete cytoplasmic granules, and in electron micrographys it was seen to be located in the lysosome-like dense bodies of 0.4-0.6 mum diameter which are normally present in the cyt ... | 1975 | 49208 |
| the origin of fornix fibers which project to the mammillary bodies in the rat: a horseradish peroxidase study. | | 1975 | 49209 |
| demonstration of nigrothalamic connections in the cat by retrograde axonal transport of horseradish peroxidase. | | 1975 | 49210 |
| caveola--vesicle complexes in the plasmalemma of erythrocytes infected by plasmodium vivax and p cynomolgi. unique structures related to schüffner's dots. | erythrocytes infected with plasmodium vivax and p cynomolgi, characterized by schüffner's dots on giemsa-stained thin films, were studied by electron microscopy and immunocytochemistry. a caveola-vesicle complex, which consisted of a caveola surrounded by vesicles, in an alveolar fashion, formed along the erythrocyte plasmalemma. horseradish-peroxidase-labeled immunoglobulin from a monkey infected with p vivax bound to the vesicle membrane. cationized ferritin appeared within the vesicles after ... | 1975 | 50017 |
| projections to the gyrus proreus from the brain stem tegmentum (locus coeruleus, raphe nuclei) in the cat, demonstrated by retrograde transport of horseradish peroxidase. | | 1975 | 50117 |
| [uptake and transport of exogenous peroxidase in the masticatory muscles of cats. localization of ganglionic neurons]. | retrograde axonal transport of horseradish peroxydase (hrp) has been used to trace the cells of origin of proprioceptive fibers in jaw-closing and jaw-opening muscles. after injection of hrp in young cats' masticatory muscles (masseteric, temporal, pterygoid, mylohyoid and digastric) labelled neurons were found in the ipsilateral semi-lunar ganglion and trigeminal mesencephalic nucleus. it is concluded that sensory endings are present in jaw-opening as in jaw-closing muscles; possibly the affere ... | 1975 | 50149 |
| [uptake and transport of exogenous peroxidase in the masticatory muscles of the cat. localization of motor neurons]. | in the present work, a topographical localization of masticatory muscle motoneurons was undertaken. horseradish peroxydase injected in each muscle can be transported in the retrograde direction to the corresponding motoneurons cell bodies. jaw-closing muscle motoneurons were identified in the dorsal part of the motor trigeminal nucleus whereas jaw-opening muscle motoneurons were observed in the ventro-medial region. | 1975 | 50152 |
| studies on the cerebellar projections from the main and external cuneate nuclei in the cat by means of retrograde axonal transport of horseradish peroxidase. | the cerebellar projections from the main and external cuneate nuclei in the cat have been studied by means of retrograde axonal transport of horseradish peroxidase. the main projection from the external cuneate nucleus (ecn) is to the intermediate and, possibly, the small lateral part of lobule v and to the paramedian lobule on the ipsilateral side. the projection from the ecn to the cerebellar regions mentioned is topographically organized. cells in the caudal part of the ecn send their axons t ... | 1975 | 50867 |
| the identification of striatal and pallidal neurons projecting to substantia nigra. an experimental study by means of retrograde axonal transport of horseradish peroxidase. | | 1975 | 51667 |
| retrograde transport of horseradish peroxidase in transected axons. ii. relations between rate of transfer from the site of injury to the perikaryon and onset of chromatolysis. | the sciatic nerves of 2--3 month-old mice, rats and rabbits were crushed and at the same time horseradish peroxidase (hrp) was applied around the crushed region at mid-thigh level. at varying time intervals thereafter, hrp was seen in neurons of the corresponding spinal ganglia and levels of the spinal cord. the rate of ascent of hrp appeared to be similar in the different animals and to be independent of the length of the nerve. the accumulation of hrp was more conspicuous in small than large n ... | 1975 | 53277 |
| localization of laryngeal motor neurons in the kitten. | in a series of 12 newborn kittens, horseradish peroxidase (hrp) was used to trace retrograde axoplasmic flow in the motor neurons to laryngeal muscles. the animals were sacrificed 24 hours after injection of hrp into a specific laryngeal muscle, and the brain stems were stained for peroxidase. this clear-cut colorimetric method permitted the localization of the motor neurons in two nuclei of the ipsilateral brain stem. these are the nucleus ambiguus and the retrofacial nucleus. the primary sourc ... | 1975 | 53770 |
| electron microscopic demonstration of immunoglobulin deposition in rheumatoid cartilage. | horseradish peroxidase (hrpo) conjugated with goat antihuman igg, goat antihuman igm, and aggregated human igg has been used as a enzymatic marker to stain igg, igm, and rheumatoid factor in rheumatoid cartilage. when hrpo-anti igg and hrpo-anti igm were used, immunoglobulin deposits were not observed in nonrheumatoid cartilage. however 7 of 8 rheumatoid cartilage specimens stained with hrpo-anti igg showed electron-dense deposits. three rheumatoid specimens stained with hrpo-anti igm showed sim ... | 1975 | 54168 |
| rôle of axonal transport in maintaining central synaptic connections. | synaptic boutons on the somata of rat hypoglossal neurones have been stained with zinc iodide-osmium, and counted using light microscopy. two weeks after silastic cuffs containing colchicine or vinblastine were applied to the hypoglossal nerve, the number of boutons on parent nerve cell bodies was significantly lower than controls. these drugs inhibited retrograde axonal transport of horseradish peroxidase, but did not affect neuromuscular conduction or the number of axons in the nerve trunk. cu ... | 1975 | 54262 |
| retrograde transpost of horseradish peroxidase in afferent and efferent neurons from masseter muscle in the rat. | | 1975 | 54884 |
| tracing axons and axon collaterals of spinal neurons using intracellular injection of horseradish peroxidase. | intracellular injection and subsequent histochemical localization of horseradish peroxidase have been used to stain the soma, dendrites, axons, and axon collaterals of spinalcervical tract neurons and unidentified dorsal horn neurons in the cat. this technique may be used in combination with the intracellular injection of procion yellow to demonstrate by light microscopy connections between physiologically typed vertebrate neurons. | 1976 | 54936 |
| electron microscopic immunohistochemical identification of endothelial cells in the rabbit. | antibody to tissue factor apoprotein was adsorbed against gamma-globulin and coupled to horseradish peroxidase; this complex was applied to various rabbit tissues. the distribution of the peroxidase marker then was observed by electron microscopy. we examined fixed or frozen sections, as well as breis of aorta, vena cava, brain, heart, lung, liver, spleen, kidney, bone marrow, mesothelial gut lining, erythrocytes, and platelets. all endothelial cells that had been exposed to the antibody complex ... | 1976 | 55315 |
| immunochemical localization of in vivo bound immunoglobulins in pemphigus vulgaris epidermis. employment of a peroxidase-antiperoxidase multistep technique for light and electron microscopy. | a multi-step immunocytochemical method utilizing horseradish peroxidase (hrp) as an immunological marker was employed to demonstrate, at the ultrastructural level, igg antiepithelial autoantibodies bound in vivo to pemphigus epidermis, and circulating igg antibodies, using monkey oesophagus as substrate. to demonstrate igg the following antisera were employed in sequential steps: goat antihuman igg; rabbit antigoat igg; goat-anti-hrp-serum. after incubating with the final antigen, hrp, the latte ... | 1975 | 56157 |
| critical factors involved in the demonstration of horseradish peroxidase retrograde transport. | | 1976 | 56209 |
| projection of thalamic neurons to cat primary vestibular cortical fields studied by means of retrograde axonal transport of horseradish peroxidase. | the two vestibular cortical projection areas in the anterior suprasylvian sulcus and post-cruciate dimple regions were defined by evoked potential technique in anaesthetized cats. the thalamic location of neurons with axon terminals in these fields was determined using the method of retrograde axonal transport of horseradish peroxidase. the ascending vestibular pathway appeared to be separated also at the thalamic level, where cells in the ventroposterolateral nucleus were found to project to th ... | 1976 | 56281 |
| concanavalin a receptors on the cell membrane of trypanosoma cruzi. | epimastigotes and trypomastigotes of t. cruzi obtained from acellular culture as well as bloodstream trypomastigotes agglutinate with concanavalin a (con a). con a-binding sites were also localized on the cell membrane by using the con a-horseradish peroxidase-diaminobenzidine method. passage of epimastigotes and trypomastigotes from acellular culture through deae-cellulose column did not affect con a-binding sites as detected by agglutination and electron miscroscopy. | 1978 | 28652 |
| tetramethyl benzidine for horseradish peroxidase neurohistochemistry: a non-carcinogenic blue reaction product with superior sensitivity for visualizing neural afferents and efferents. | tetramethyl benzidine (tmb) is a presumptively non-carcinogenic chromogen which yields a blue reaction-product at sites of horseradish peroxidase activity. sixty-six distinct procedures were performed in rats and monkeys in order to determine the optimal incubation parameters for tmb. as a result, a procedure is recommended whose sensitivity greatly surpasses that of a previously described benzidine dihydrochloride method. indeed, the sensitivity of this new method in demonstrating retrograde tr ... | 1978 | 24068 |
| ultrastructural differences between the preterminal nerve fibres and their endings in the mechanoreceptors, with special reference to their degeneration and mode of uptake of horseradish peroxidase. | | 1976 | 56762 |
| a note on the method of retrograde transport of horseradish peroxidase as a tool in studies of afferent cerebellar connections, particularly those from the inferior olive; with comments on the orthograde transport in purkinje cell axons. | 1. injections of horseradish peroxidase (hrp) suspension were made in the cerebellar cortex of cats (most often the paramedian lobule). the staining of the cerebellar cortex and the ensuing labeling of neurons in the inferior olive were studied in experiments with variations of concentration of hrp, amounts of fluid injected, survival time and age of the animals. light microscopical studies were supplemented with electron microscopical observations. the folded cerebellar cortex offers particular ... | 1976 | 57065 |
| movement of horseradish peroxidase in rabbit submandibular glands after ductal injection. | horseradish peroxidase (hrp) has been used as a tracer to study movements of solutions injected retrogradely via the duct of submandibular glands in rabbits. 0.1 ml of solution was injected either manually or by a constant hydrostatic pressure, and the subsequent distribution of hrp in the gland and duct at different times after injection has been examined histochemically at light and electron microscopical levels. shortly after the injections, strong interstitial staining for peroxidase resulte ... | 1976 | 57956 |
| a guide to the synaptic analysis of the neuropil. | a morphological analysis of the organization of the gray matter in the central nervous system depends on the discovery of consistent repetitive patterns. without these, the gray matter remains a chaotic jungle. an hypothesis derived from the study of a few simple regions has been developed to serve as a guide in finding these patterns. it states that all nerve fibers and terminals arising from a particular group of nerve cells, or, more precisely, a particular nerve cell type, display similar ax ... | 1976 | 59655 |
| differences between alpha and gamma motoneurons labeled with horseradish peroxidase by retrograde transport. | | 1976 | 60166 |
| the olivocerebellar projection in the cat studied with the method of retrograde axonal transport of horseradish peroxidase. iii. the projection to the vermal visual area. | horseradish peroxidase (hrp) was injected separately in one of the cerebellar lobules vi, viia, viib, viiia or viiib (together corresponding to the vermal visual area) in 17 cats. after 1-3 days the distribution of labeled cells in the inferior olive was mapped. in spite of some overlapping it is clear that the various lobules of the vermal visual area receive fibers from separate parts of a horseshoeshaped region in the caudal half of the contralateral medial accessory olive (fig. 5c). the proj ... | 1976 | 61211 |
| movement of horseradish peroxidase in the anterior parts of the eye [proceedings]. | | 1975 | 61704 |
| retrograde transport of horseradish peroxidase in transected axons. 3. entry into injured axons and subsequent localization in perikaryon. | horseradish peroxidase (hrp) applied to crushed mouse sciatic nerves diffused through the damaged perineurium into the endoneurium. in the injured area, hrp passed into damaged myelinated and unmyelinated axons forming columns of reaction product, which extended for several millimeters proximally to the lesion. ultrastructurally, hrp adhered to the inner surface of the axoplasm and to the surfaces of neurotubules and neurofilaments in such columns. at more proximal levels axons contained hrp in ... | 1976 | 61782 |
| afferents to the rat substantia nigra studied with horseradish peroxidase, with special reference to fibres from the subthalamic nucleus. | | 1976 | 61785 |
| a horseradish peroxidase study of the cortico-olivary projection in the cat. | | 1976 | 61790 |
| direct projections from the mesodiencephalic midline areas to the pericruciate cortex in the cat: an experimental study with the horseradish peroxidase method. | | 1976 | 61792 |
| nigrothalamic projections in the rat as demonstrated by orthograde and retrograde tracing echniques. | the nigrothalamic projection of the rat was investigated by means of horseradish peroxidase (hrp) histochemistry and by the autoradiographic tracing technique. the use of these techniques has permitted an extension of previous descriptions by identifying a neuron population in the substantia nigra, pars reticulata (snr) as the nigrothalamic cells of origin. these calls (20-25 mu) were multipolar and were distributed throughout the snr. there was a tendency however for more labelled neurons to be ... | 1976 | 61793 |
| origins of axons in the cat's acoustic striae determined by injection of horseradish peroxidase into severed tracts. | origins and terminations of fibers of the dorsal and intermediate acoustic striae were studied by surgically severing these tracts and injecting hrp into the incision. this procedure results in filling the severed axons with hrp. filled axons were traced to cell groups of origin and to some terminations of the acoustic striae. hrp-labeled terminals were found in the cochlear nuclei as well as in periolivary cell groups. filling of cells with hrp ranged from being complete, resulting in golgi-lik ... | 1976 | 61976 |
| the origin of the pretecto-olivary tract. a study using the horseradish peroxidase method. | | 1976 | 62601 |
| the use of glutaraldehyde-conjugated horseradish peroxidase-bovine serum albumin in the visualization of concanavalin a binding to tissue sections of human colonic tumor. | a method is reported for the preparation of glutaraldehyde cross-linked horseradish peroxidase conjugates for the visualization of concanavalin a binding to frozen sections. the binding of concanavalin a to tissue sections of human colonic tumor is used to compare staining by horseradish peroxidase conjugates and monomers. without decreasing specificity, the use of progressively larger molecular weight conjugates provides progressively greater intensity of staining of the epithelial components o ... | 1976 | 62892 |
| comparative immunoenzymatic localization of prolactin and growth hormone in human and rat pituitaries. | twelve human and twelve rat pituitaries were stained by an immunohistochemical method using a rabbit anti-ovine prolactin serum, a rabbit anti-human growth hormone serum and a sheep anti-rabbit immunoglobulin serum conjugated with horseradish peroxidase. on the same pituitary section, growth hormone cells were stained brown by using 3-3'-diaminobenzidine as peroxidase substrate, and prolactin cells were stained purplish blue by using 4-chloro-1-naphtol. growth hormone cells outnumbered prolactin ... | 1976 | 61240 |
| the climbing fibers of the cerebellar cortex, their origin and pathways in cat. | the sources and pathways of the climbing fibers to the cerebellar posterior vermis were studied with comibined electrophysiological and anatomical methods in cats. recording from identified cerebellar purkinje cells, monosynaptic climbing fiber (cf) responses have been obtained both for stimulation of the inferior olive (io) and various parts of the brain stem (bs). cf responses were found to of three types, io only, bs only or both io and bs. however the responses to bs stimulation were very fe ... | 1976 | 63384 |
| horseradish peroxidase: an improvement in intracellular staining of single, electrophysiologically characterized neurons. | | 1976 | 63386 |
| the anatomical organization of the cerebello-olivary projection in the cat. | the cerebello-olivary pathway in the cat has been examined using orthograde and retrograde neuroanatomical tracing techniques. the orthograde transport of 3h-leucine from injection sites in the deep cerebellar nuclei labeled dentate and interpositus projections to the rostral two-thirds of the contralateral inferior olivary complex. these projections are topographically organized, with the dentate nucleus projecting to the principal olivary nucleus and the posterior and anterior interpositus nuc ... | 1976 | 63472 |
| immune complex receptors on cell surface. i. ultrastructural demonstration of macrophages. | a method is described for ultrastructural localization of immune complex receptors on the surface of viable peritoneal exudate cells. the technique entails incubation with a soluble complex of horseradish peroxidase (hrp) and specific antibody to hrp at 4 degrees c followed by exposure to diaminobenzidine and processing for electron microscopy. the bound immune complexes were evident as focal deposits of hrp reaction product, adhering closely to the external surface of macrophages with an uninte ... | 1976 | 60441 |
| anti-streptococcal antibodies reacting with brain tissue. ii. utrastructural studies. | the reaction of rabbit anti-streptococcus pyogenes type 24 with mouse brain has been studied at the ultrastructural level using horseradish peroxidase labelled antiglobulin. it is shown that these antibodies, and by inference those found in sydenham's chorea, react with the fibrous astrocyte. the cross-reacting antigen is the glial filament or a closely associated cytoplasmic constituent. | 1976 | 60120 |
| identification of cells or origin of non-primary afferents to the dorsal column nuclei of the cat. | in order to identify cells of origin in the spinal cord of non-primary afferents to the dorsal column nuclei (dcn), the retrograde transport of horseradish peroxidase (hrp) has been utilized in adult cats. 10 to 30% hrp was injected bilaterally (0.6 mul per side) in the dorsal medulla of nine cats. in most instances the spread of the injected enzyme extended a few millimeters rostrocaudally and infiltrated the dcn as well as other nuclei and fiber tracts. labelled cells in these cases are numero ... | 1977 | 64365 |
| the development of the isthmo-optic tract in the chick, with special reference to the occurrence and correction of developmental errors in the location and connections of isthmo-optic neurons. | the development of the centrifugal projection to the chick retina in the isthmo-optic tract (iot) has been studied by the retrograde transport of the enzyme marker horseradish peroxidase (hrp) injected into the eye at various times during the incubation period. some neurons in the isthmo-optic nucleus (ion--the cells of origin of the iot) can first be labeled from the eye following injections on the tenth day of incubation; after injections on the twelfth day or later, about 95% of the neurons c ... | 1976 | 58875 |
| functional organization of the vestibular input to the anterior and posterior cerebellar vermis of cat. | 1. responses evoked by electrical stimulation (auditory division of the viiith nerve sectioned chronically) and natural stimulation of the vestibular apparatus were recorded in the anterior and posterior cerebellar vermis of cats anesthetized with ketamine or nembutal. under ketamine the functional state of the cerebellar cortex was similar to that of the decerebrate or encéphale isolé preparation. 2. vestibular-evoked responses were found bilaterally throughout the vermis (lob. i-x) and parts o ... | 1977 | 65291 |
| the olivocerebellar projection in the cat studied with the method of retrograde axonal transport of horseradish peroxidase. iv. the projection to the anterior lobe. | following injections of horseradish peroxidase (hrp) in the cerebellar cortex of the anterior lobe of the cat, the distribution of labeled cells in the inferior olive was mapped. the findings largely confirm those made previously in studies of olivary retrograde cell loss following cerebellar ablations (brodal, '40b). in addition, they reveal further olivary areas projecting onto the anterior lobe, and permit a more detailed analysis of the pattern in this projection. concerning major points the ... | 1977 | 65365 |
| brain stem afferents to the fastigial nucleus in the cat demonstrated by transport of horseradish peroxidase. | although retrograde and anterograde degeneration studies have provided important information concerning brain stem afferents to the fastigal nucleus (fn), these data may be incomplete and should be confirmed by axonal transport methods. attempts were made to inject horseradish peroxidase (hrp) unilaterally into the fn in a series of adult cats. animals were perfused with dextran and a fixative solution of paraformaldehyde and glutaraldehyde in 0.1 m phospate buffer. representative sections were ... | 1977 | 65366 |
| the vestibular complex of the american opossum didelphis virginiana. ii. afferent and efferent connections. | we have demonstrated the connectivity of the opossum's vestibular nuclei using degeneration, autoradiographic and horseradish peroxidase techniques and have found it to be generally comparable to that reported for the cat. apart from the primary input described in part i of our study, the cerebellum provides the major source of afferent connections to the vestibular complex. axons from the cerebellar cortex distribute mainly to vestibular areas which receive no primary afferent projections, e.g. ... | 1977 | 65367 |
| ultrastructural visualization of cellular carbohydrate components by means of lectins on ultrathin glycol methacrylate sections. | a method for the visualization of cellular carbohydrate components by both light and electron microscopy using lectins on glycol methacrylate sections is proposed. this method, which is an application of the lectin-peroxidase affinity technique, solves the problem of limited penetration when it is attempted to demonstrated lectins receptors within the tissue block. following partial dissolution of glycol methacrylate from thin sections using alcohol, they are incubated successively with lectin ( ... | 1977 | 65417 |
| organization of the facial nerve nucleus in the cat. | the location of efferent neurons in the brain stem of newborn kittens supplying the facial muscles was studied by means of horseradish peroxidase (hrp) used as a tracer of retrograde protein transport. three to 15 microliters containing 0.3 to 1.5 mg of hrp was injected into the orbicularis oris and orbicularis oculi muscles of six kittens and in the seventh kitten, the ventral ramus of the facial nerve was transected and hrp was instilled on the cut end of the nerve. after a survival time of 24 ... | 1977 | 65707 |
| brain stem projections to lobule vii of the posterior vermis in the squirrel monkey: as demonstrated by the retrograde axonal transport of tritiated horseradish peroxidase. | | 1977 | 66085 |
| localization of abductor and adductor fibers in the kitten recurrent laryngeal nerve: use of a variation of the horseradish peroxidase tracer technique. | | 1977 | 66152 |
| identification of different size motoneurons labeled by the retrograde axonal transport of horseradish peroxidase. | three main groups of motoneurons of different size have been labeled in adult cats by using the method for retrograde axonal transport following injection of horseradish peroxidase in the medial gastrocnemius and soleus muscles. in particular small, medium-size and large neurons which probably correspond respectively to gamma, small alpha and large alpha motoneurons innervating the calf muscles, have been identified and the corresponding area measured. | 1977 | 67590 |
| the organization of thalamocortical relay neurons in the rat ventrobasal complex studied by the retrograde transport of horseradish peroxidase. | | 1977 | 68039 |
| light and electron microscopic observations on the anterograde transport of horseradish peroxidase in the optic pathway in the mouse and rat. | | 1976 | 55292 |
| [study of the vagal innervation of the cat stomach by means of retrograde axoplasmic transport of horseradish peroxidase--on the localization of the origins of the preganglionic fibers]. | | 1977 | 68782 |
| retrograde axonal transport of horseradish peroxidase. uptake at mouse neuromuscular junctions following systemic injection. | eye muscles were examined histochemically for the presence of horseradish peroxidase (hrp) activity after intravenous injections of large doses of this tracer protein. hrp had penetrated through the blood vessels and diffused into the areas of motor end plates, where it outlined axon terminals at the synaptic clefts. hrp was incorporated into pinocytotic vesicles in the axons from where an intraaxonal transport in the retrograde direction to the nerve cell bodies in the brainstem followed. accum ... | 1977 | 69382 |
| demonstration of bilateral claustro-cortical connections in the cat with the method of retrograde axonal transport of horseradish peroxidase. | the claustrum of the cat was studied to determine the extent of its projection to the cerebral cortex. neurons in the bilateral dorsal claustrum are labeled by retrograde axonal transport of horseradish peroxidase (hrp) from injection sites of different neocortical areas with preponderance to the ipsilateral side. it is revealed that the dorsal claustrum projects to almost entire regions of the bilateral neocortex and that distribution of labeled neurons in the bilateral claustrum has topographi ... | 1977 | 69423 |
| identification of cells of origin of tectopontine fibers in the cat superior colliculus: an experimental study with the horseradish peroxidase method. | the pontine projections from the superior colliculus in the cat have been studied by means of retrograde axonal transport of horseradish peroxidase (hrp). following injections of hrp in the dorsolateral pontine nucleus, where the tectopontine fibers terminate, a fair number of labeled cells are found throughout the rostrocaudal extent of the ipsilateral superior colliculus. relatively few of the labeled cells are of medium size (25-40 micron in diameter), more than 80% are small (10-25 micron), ... | 1977 | 69477 |
| the effects of monocular deprivation on different neuronal classes in the lateral geniculate nucleus of the cat. | retrograde axonal transport of horseradish peroxidase (hrp) was used to identify two populations of cells in the lateral geniculate nucleus (lgn) of the cat. hrp was injected into area 17 and 18 separately in the same animals, and the neuronal somata giving rise to thalamo-cortical axons, identified by the presence of granular hrp reaction product within them, were measured. the mean size of lgn neurones labelled by injections in area 17 ("17-relay" cells) was less than of neurones filled from a ... | 1977 | 69546 |
| ultrastructural immunoenzymatic study of alpha-fetoprotein-producing cells in the human fetal liver. | cells producing alpha-fetoprotein in human fetal liver have been studied with specific horseradish peroxidase labeled immunoglobulins. under light microscopy, the alpha-fetoprotein is strictly localized in the cytoplasm of certain hepatocytes, distributed randomly in the hepatic lobule. ultrastructural examination of thesame cells shows that the alpha-fetoprotein is present within the cytoplasm. ultrastructural differences are described in hepatocytes according to whether or not the cell is prod ... | 1977 | 69654 |
| axoplasmic transport in ocular hypotony and papilledema in the monkey. | orthograde and retrograde axoplasmic transport were studied in optic nerve heads of seven hypotensive macaca fascicularis eyes. orthograde transport was studied by radioautography after intravitreal radioisotope injections. retrograde transport was studied in the same eyes by horseradish peroxidase injection into the dorsal lateral geniculate nuclei or optic tracts. three eyes had developed marked papilledema before injections. orthograde axoplasmic transport was blocked in swollen axons of the ... | 1977 | 70200 |
| bidirectional movement of horseradish peroxidase and the demonstration of reciprocal thalamocortical connections. | | 1977 | 70257 |
| evidence for direct synaptic interconnections between cat spinal alpha-motoneurons via the recurrent axon collaterals: a morphological study using intracellular injection of horseradish peroxidase. | by using intracellular injection of horseradish peroxidase into cat triceps surae alpha-motoneurons we have been able to trace the motor axons as well as the recurrent motor axon collaterals with their synaptic terminals both with the light and electron microscope. in addition to the expected projection to the 'renshaw cell area', the triceps surae motor axon collaterals were frequently found to terminate within the motor nuclei, where at least some of the terminals made direct synaptic contact ... | 1977 | 70258 |
| factors affecting retrograde axonal transport of horseradish peroxidase in the visual system. | | 1976 | 55291 |
| prenatal development of central optic pathways in albino rats. | the development of the central optic projections in albino rat fetuses has been studied using light and electron microscopic degeneration techniques and the horseradish peroxidase method for demonstrating axonal projections of neurons. the first optic axons to reach the region of the optic chiasm arrive at day 15. by day 16, a substantial optic chiasm is seen and the optic tract can be traced into the epithalamus, having first passed through the ventral lateral geniculate nucleus and a thin lami ... | 1976 | 54370 |
| [projections of bulbar reticular nuclei and raphe nuclei to lobules vi and vii of cat cerebellar cortex]. | injections of horseradish peroxidase were made in lobules vi and vii of the cerebellar vermis (in the cat). serial frontal sections of the brain stem reveal that the lateral and paramedian nuclei were essentially projected onto lobule vi, and the projections from the raphe nuclei onto lobule vii. | 1977 | 70281 |
| preganglionic innervation of the adrenal gland of the rat: a study using horseradish peroxidase. | | 1975 | 53160 |
| a study of the dynamics of retrograde transport and accumulation of horseradish peroxidase in injured neurons. | the phenomenon of retrograde intraaxonal transport of extracellular markers introduced at the level of the axon terminal has been suggested as a possible mechanism of communication between the axon terminal and the neuron cell body. we tested the hypothesis that communication after axotomy might consist of a change in the rate of uptake or of transport of material by injured neurons. small lesions were made with a needle in one retinal quadrant of chicks and immediately afterwards horseradish pe ... | 1975 | 53088 |
| movement of horseradish peroxidase in the cornea, sclera and the anterior uvea. | the anterior chambers of rabbit, monkey and human eyes were perfused with horseradish peroxidase. the perfusion was started immediately after enucleation of the monkey and human eyes, while the rabbit eyes were perfused in situ after the animals had been killed. comparative results were obtained after 1 h or perfusion and were mainly based on frozen sections. intensive staining was found in the descemet's membrane, the sclera and the iridial stroma, considerably more than in the corneal stroma a ... | 1977 | 71844 |
| arborization of initial axon collaterals of spinocervical tract cells stained intracellularly with horseradish peroxidase. | the initial axon collateral arborizations of two feline spinocervical tract cells have been analysed after intracellular staining with horseradish peroxidase. the images of the cells were reconstructed in the light microscope from transverse serial sections. the cell bodies were situated in laminae iv or iii of the lumbar dorsal horn. each cell exhibited one initial collateral. these emerged from the parent axons at distances of about 310 and 580 micron, respectively, from the cell body. the col ... | 1977 | 71934 |
| the pontine projection to the crebellar anterior lobe. an experimental study in the cat with retrograde transport of horseradish peroxidase. | | 1977 | 72000 |
| detection of epstein-barr virus antigens with enzyme-conjugated antibody. | monospecific conjugated (fluorescein isothiocynate and horseradish peroxidase) goat antisera, prepared against three human immunoglobulin classes, im (mu), igg (gamma) and iga (alpha), were compared for their ability to detect human ig classes possessing specificity for epstein-barr virus (ebv) viral capsid antigens (vca) in a chronically infected human lymphoid cell line, p3j-hrik. it was determined that the enzyme system was significantly more sensitive than immunofluorescence in detecting mos ... | 1977 | 72045 |
| purification of mouse alpha1-fetoprotein and preparation of specific peroxidase conjugates for its cellular localization. | mouse alpha1-fetoprotein (afp) was isolated from amniotic fluid by immunoadsorbent columns and preparative electrophoresis. specific antibodies were isolated from monospecific hyperimmunsera by use of immunoadsorbents, and subsequently coupled with horseradish peroxidase. at the light microscopical level, purified antibody-peroxidase conjugates were used for the cellular localization of afp in fetal liver by direct and indirect staining methods. fixatives containing ethanol or aldehydes were tri ... | 1975 | 49343 |
| the olivocerebellar projection studied with the method of retrograde axonal transport of horseradish peroxidase. v. the projections to the flocculonodular lobe and the paraflocculus in the rabbit. | hrp was injected in the flocculonodular lobe and the paraflocculus in the rabbit to determine the areas of the inferior olive which project onto these cerebellar regions. following injections in the flocculus labeled cells occurred in the dorsal cap and the rostralmost tip of the medial accessory olive. following injections in the nodulus labeled cells were likewise found in the dorsal cap, but in addition in the rostralmost part of the dorsomedial cell column and the adjoining part of the media ... | 1977 | 72080 |
| the olivocerebellar projection in the cat studied with the method of retrograde axonal transport of horseradish peroxidase. vi. the projection onto longitudinal zones of the paramedian lobule. | microinjections (30-50 nl) of a horseradish peroxidase (hrp) suspension of 25% (wt./vol.) were made in different folia of the paramedian lobule of cats, and the sites of occurrence of labeled cells in the inferior olive were precisely determined. in each case only a small number of cells are labeled, aggregated in a minute area. the labeled cells are found within three only of the four olivary areas previously determined (brodal et al., '75) to project onto the paramedian lobule (fig. 1): one ar ... | 1977 | 72081 |
| salivatory neurons innervate the submandibular and sublingual glands in the rat: horseradish peroxidase study. | | 1977 | 72589 |
| cerebeller afferents from neurons in motor nuclei of cranial nerves demonstrated by retrograde axonal transport of horseradish peroxidase. | | 1977 | 72590 |
| the retrograde transport method. | after an injection of horseradish peroxidase into the brain, the enzyme product can later be found in cell bodies of neurons which project axons to the injection site. the use of the tracer has now been adapted as a means of identifying neurons on the basis of their efferent fiber projections. in this review particular attention is paid to characteristics of the phenomenon of retrograde transport, such as diffusion of the marker from the injection site and the region of effective uptake, uptake ... | 1975 | 48482 |
| use of peroxidatic-enzyme staining to enhance resolution of cultured mammalian cells under phase microscopy. | staining of glutaraldehyde-fixed mammalian cells with peroxidatic enzymes (horseradish peroxidase or horse heart cytochrome c) greatly enhances resolution of their structure under phase microscopy. the topography of cell processes and regions of intercellular contact and overlapping is resolved precisely, even in dense cultures mounted in media which ordinarily do not permit clear demonstration of these areas. the technique is therefore a useful aid to the study of cultured cells with phase opti ... | 1975 | 46631 |
| intracellular staining of cortical neurons by pressure microinjection of horseradish peroxidase and recovery by core biopsy. | | 1978 | 73474 |
| noradrenaline innervation of the spinal cord studied by the horseradish peroxidase method combined with monoamine oxidase staining. | the origin of the spinal cord noradrenaline (na) has been investigated by means of the horseradish peroxidase (hrp) method, combined with monoamine oxidase staining (glenner) to identify the na neurons. following the injection of hrp to the various levels of rat spinal cord, cervical to sacral cord, a1-3, 5-7 na neuron groups were labeled with hrp. they showed almost the same distribution pattern regardless of difference in the injected segment. labeled na neurons in a6 were concentrated in the ... | 1977 | 74341 |
| the pontine projection to the flocculonodular lobe and the paraflocculus studied by means of retrograde axonal transport of horseradish peroxidase in the rabbit. | the occurrence and distribution of labeled cells in the pontine nuclei were mapped following injections of small amounts of horseradish peroxidase (0.05-0.5 microliter, 50% suspension) in the flocculus, nodulus and the dorsal and ventral paraflocculus in adult albino rabbits. while no labeled cells were found in the pontine nuclei following injections in the nodulus, some were present following injections in the flocculus and a great number following injections in the paraflocculus. the projecti ... | 1977 | 74343 |
| further studies on hybrid cell-surface antigens associated with human chromosome 11. | a new human immunogenetic cell-surface activity associated with human chromosome 11 in the al human-chinese hamster ovary cell hybrid is described. like a1, but not a2, it is present on the human erythrocyte. by mutagenesis and selection, specific, stable, variants of the al hybrid have been prepared exhibiting various combinations of a1, a2, a3, and lactic dehydrogenase a activities. the antigens of the al system can be demonstrated by the horseradish peroxidase system which offers a promising ... | 1977 | 74861 |
| corticothalamic neurons and thalamocortical terminal fields: an investigation in rat using horseradish peroxidase and autoradiography. | subsequent to thalamic injections in rats of horseradish peroxidase (hrp) alone or hrp and [3h]leucine in combination, the cells of origin of the corticothalamic projections and the terminal fields of the thalamocortical projections were identified. hrp-labeled corticothalamic neurons were uniformly found in layers v and vi. they were medium to small in size and always pyramidal in shape with the larger neurons being found in layer v. on the other hand, 3 different patterns for the distribution ... | 1975 | 46175 |
| neurons in and near nucleus caudalis with long ascending projection axons demonstrated by retrograde labeling with horseradish peroxidase. | | 1978 | 75038 |