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resonance raman spectra of plastocyanin and pseudoazurin: evidence for conserved cysteine ligand conformations in cupredoxins (blue copper proteins).new resonance raman (rr) spectra at 15 k are reported for poplar (populus nigra) and oleander (oleander nerium) plastocyanins and for alcaligenes faecalis pseudoazurin. the spectra are compared with those of other blue copper proteins (cupredoxins). in all cases, nine or more vibrational modes between 330 and 460 cm-1 can be assigned to a coupling of the cu-s(cys) stretch with cys ligand deformations. the fact that these vibrations occur at a relatively constant set of frequencies is testimony t ...19911932014
insect tolerance of transgenic populus nigra plants transformed with bacillus thuringiensis toxin gene.leaves and stem segments of populus nigra were transformed with a. tumefaciens lba4404 harboring a binary vector containing chimeric genes of npt and 35s-omega-b.t. toxin-nos. nineteen regenerated kanamycin resistant plants were analyzed by dna hybridization, out of which 10 were shown to be the candidates of transgenic plants. insect tolerance tests showed that the transgenic plants were toxic to two lepidopteran pests, lymantria dispar l. and apocheimia cinerarius erschoff. based on the result ...19938061230
differential toxicity of leaf litter to dipteran larvae of mosquito developmental sites.the relative toxicity of leaf litter to nematocerous dipteran larvae characteristic of mosquito developmental sites was investigated. culicidae, chironomidae, and simuliidae taxa originating from alpine hydrosystems were tested together with two laboratory nonindigenous culicid taxa. bioassays indicate that ingestion of 10-month-old decaying leaves from alnus glutinosa, populus nigra, and quercus robur by larvae is more deleterious for aedes aegypti, a. albopictus, culex pipiens, simulium varieg ...200010631052
a study of metal distribution from lignite fuels using trees as biological monitors.concentrations of five metals (cd, cu, mn, fe, zn) were determined in tree leaves collected from an area with large coal-fired plants in ptolemais, macedonia, greece. the analyses were carried out with flame atomic absorption spectroscopy. mean heavy metal content in the tree leaves is, in descending order, fe>mn>zn>cu>cd. in populus nigra and salix babylonica the dense piled leaves and the widespread root system are the reasons for high heavy metal uptake. conifer trees pinus nigra and juniperu ...200111161674
eurobionet: a pan-european biomonitoring network for urban air quality assessment.eurobionet, the 'european network for the assessment of air quality by the use of bioindicator plants', is an eu-funded cooperative project currently consisting of public authorities and scientific institutes from 12 cities in 8 countries. in 2000, the bioindicator plants tobacco (nicotiana tabacum bel w3), poplar (populus nigra 'brandaris'), spiderwort (tradescantia sp. clone 4430), italian rye grass (lolium multiflorum italicum) and curly kale (brassica oleracea acephala) were exposed to ambie ...200212094534
trace element biomonitoring by leaves of populus nigra l. from western anatolia, turkey.the west anatolian region is one of the most important areas for industrialization, population density and traffic in turkey. therefore, the leaves of populus nigra l. (salicaceae), which are used as biomonitor to investigate the levels of the trace elements cd, pb, fe, zn and cu, were sampled at 30 locations having different pollution levels in the region. the concentrations of elements were determined by atomic absorption spectrometry. the samples were collected at both reference and contamina ...200516459554
genetic manipulation of the metabolism of polyamines in poplar cells. the regulation of putrescine catabolism.we investigated the catabolism of putrescine (put) in a non-transgenic (nt) and a transgenic cell line of poplar (populus nigra x maximowiczii) expressing a mouse (mus musculus) ornithine (orn) decarboxylase (odc) cdna. the transgenic cells produce 3- to 4-fold higher amounts of put than the nt cells. the rate of loss of put from the cells and the initial half-life of cellular put were determined by feeding the cells with [u-(14)c]orn and [1,4-(14)c]put as precursors and following the loss of [( ...200211950994
increased leaf area expansion of hybrid poplar in elevated co2. from controlled environments to open-top chambers and to face.we examined the response of hybrid poplar to elevated co2 in contrasting growth environments: controlled environment chamber (ce). open-top chamber (otc) and poplar free air co2 enrichment (popface) in order to compare short versus long-term effects and to determine whether generalisations in response are possible for this fast growing tree. leaf growth, which for poplar is an important determinant of stemwood productivity was followed in all environments, as were the determinants of leaf growth ...200111789926
transgenic manipulation of the metabolism of polyamines in poplar cells.the metabolism of polyamines (putrescine, spermidine, and spermine) has become the target of genetic manipulation because of their significance in plant development and possibly stress tolerance. we studied the polyamine metabolism in non-transgenic (nt) and transgenic cells of poplar (populus nigra x maximowiczii) expressing a mouse orn decarboxylase (odc) cdna. the transgenic cells showed elevated levels of mouse odc enzyme activity, severalfold higher amounts of putrescine, a small increase i ...200111299393
genetic variation of the riparian pioneer tree species populus nigra. ii. variation in susceptibility to the foliar rust melampsora larici-populinapartial resistance of populus nigra l. to three races of the foliar rust melampsora larici-populina kleb. was studied in a field trial and in laboratory tests, using a collection of p. nigra originating from different places throughout france. no total resistance was found. the partial resistance was split into epidemiological components, which proved to be under genetic control. various patterns of association of epidemiological components values were found. principal components analysis reveal ...199910336707
the effect of phenolic compounds of poplar leaves extract on cutaneous angiogenesis reaction induced in mice by human mononuclear leukocytes.the influence of ether fraction of water-soluble extract of poplar leaves (populus nigra l.) and nine phenolic acids (cinnamic and benzoic acids derivatives) on human mononuclear (mnl) leukocytes angiogenic activity was studied. ether fraction, containing mainly phenolic acids, increased cutaneous angiogenesis induced in mice by human mnl isolated from the blood of healthy donors. increase of angiogenic activity was also observed in case of caffeic, gallic, salicylic, ferulic and gentisic acids. ...19979415205
determination of roadside noise reduction effectiveness of pinus sylvestris l. and populus nigra l. in erzurum, turkey.this study was carried out to determine the noise-reduction effectiveness of the pinus sylvestris l. and populus nigra l. trees along the e-80 state highway in erzurum-turkey. noise values were taken at three measurement points; open (without trees and taken as control), the urban forests of pure p. sylvestris l. and p. nigra l. areas, and at four different distances; 0 (near the noise source), 25, 50 and 75 m from the noise source. at the distance of 25 m from the source, the noise value was 78 ...200817957489
pollen grains bind to lung alveolar type ii cells (a549) via lung surfactant protein a (sp-a).lung surfactant protein a (sp-a) is the most abundant surfactant-associated protein present in the lung. a receptor for sp-a has been shown to be present on a549 alveolar type ii cells and on other cell types, including alveolar macrophage. the sp-a receptor on a549 cells has been identified as the collection receptor, or c1q receptor, which binds several structurally-related ligands. sp-a contains c-type lectin domains, but the role of carbohydrate binding by sp-a in physiological and pathologi ...19938374060
complete amino acid sequence of plastocyanin from a green alga, enteromorpha prolifera.the complete amino acid sequence of the plastocyanin from the green alga enteromorpha prolifera has been determined by edman degradation of the intact molecule and fragments produced by enzymatic cleavage of the polypeptide chain with chymotrypsin, staphylococcus aureus protease, proline-specific endopeptidase, lys-c endopeptidase and trypsin. the molecule consists of 98 amino acid residues with a calculated relative molecular mass of 10103. the amino acid sequence of e. prolifera plastocyanin s ...19863522227
a two-dimensional electrophoresis procedure for single meristems of different forest species.high resolution two-dimensional electrophoresis, with isoelectric focusing in the first dimension and electrophoresis in sodium dodecyl sulfate in thin acrylamide gels in the second dimension, has been applied to separate the proteins of single meristems (200-300 microns) from sequoiadendron giganteum, sequoia sempervirens, pseudotsuga menziesii, picea abies, pinus pinaster, eucalyptus gunnii and populus nigra. the technique may prove an efficient tool for studying inter- and intraclonal differe ...19892776736
isolation of flavonoids from populus nigra as delta 4-3-ketosteroid (5 alpha) reductase inhibitors.inhibitors of delta 4-3-ketosteroid (5 alpha) reductase, which had been prepared from rat prostate and converted testosterone to 5 alpha-dihydrotestosterone and 4-androstene-3,17-dione, were isolated from 50% ethanol extract of populus nigra. they were identified as pinobanksin (i, 3,5,7-trihydroxyflavanone), 3,7-dimethylquercetin (ii, 3',4',5-trihydroxy-3,7-dimethoxyflavone) and pinocembrin (iii, 5,7-dihydroxyflavanone). compound iii showed the most potent inhibitory activity among them.19892632060
gene synthesis, expression, and mutagenesis of the blue copper proteins azurin and plastocyanin.genes for the blue copper proteins populus nigra var. italica plastocyanin and pseudomonas aeruginosa azurin have been constructed by a stepwise procedure. the leader sequence for azurin has been placed before the genes directing plastocyanin and azurin transport to the periplasmic space when the genes are expressed in escherichia coli. site-saturation mutagenesis has been used to alter two copper-binding residues of azurin (met-121 and his-46) and met-92 of plastocyanin. while the plastocyanin ...19911899926
effect of submergence-emergence sequence and organic matter or aluminosilicate amendment on metal uptake by woody wetland plant species from contaminated sediments.site-specific hydrological conditions affect the availability of trace metals for vegetation. in a greenhouse experiment, the effect of submersion on the metal uptake by the wetland plant species salix cinerea and populus nigra grown on a contaminated dredged sediment-derived soil and on an uncontaminated soil was evaluated. an upland hydrological regime for the polluted sediment caused elevated cd concentrations in leaves and cuttings for both species. emergence and soil oxidation after initial ...200716678320
transcriptome profiling in hybrid poplar following interactions with melampsora rust fungi.in natural conditions, plants are subjected to a combination of biotic stresses and often have to cope with simultaneous pathogen infections. in this report, we aim to understand the global transcriptional response of hybrid poplar nm6 (populus nigra x p. maximowiczii) to infection by two biotrophic melampsora fungi, melampsora larici-populina and m. medusae f. sp. deltoidae. these pathogens triggered different responses after inoculation of poplar leaves. transcript profiling using the genechip ...200919132871
population dynamics of the ectomycorrhizal fungal species tricholoma populinum and tricholoma scalpturatum associated with black poplar under differing environmental conditions.fungi combine sexual reproduction and clonal propagation. the balance between these two reproductive modes affects establishment dynamics, and ultimately the evolutionary potential of populations. the pattern of colonization was studied in two species of ectomycorrhizal fungi: tricholoma populinum and tricholoma scalpturatum. the former is considered to be a host specialist whereas t. scalpturatum is a generalist taxon. fruit bodies of both basidiomycete species were mapped and collected over se ...200616623736
hybrid poplar clones with populus maximowiczii parentage demonstrate postoviposition antibiosis to cryptorhynchus lapathi (coleoptera: curculionidae).damage caused by the poplar-and-willow borer, cryptorhynchus lapathi (l.) (coleoptera: curculionidae), is reported to vary among hybrid poplar clones. we evaluated oviposition preferences and larval success in four hybrid poplars on potted and field-planted trees. oviposition occurred somewhat less frequently and abundantly on two clones with populus maximowiczii henry parentage in field-planted and potted trees, and significantly fewer larvae survived to adulthood on those clones. no adults eme ...200516539157
assessment of populus wood chemistry following the introduction of a bt toxin gene.unintended changes in plant physiology, anatomy and metabolism as a result of genetic engineering are a concern as more transgenic plants are commercially deployed in the ecosystem. we compared the cell wall chemical composition of three populus lines (populus trichocarpa torr. & a. gray x populus trichocarpa bartr. ex marsh., populus trichocarpa x populus nigra l. and populus deltoides x populus nigra) genetically modified to express the cry3a or cry3b2 protein of bacillus thuringiensis (bt) wi ...200616452069
microbiology of wetwood: importance of pectin degradation and clostridium species in living trees.wetwood samples from standing trees of eastern cottonwood (populus deltoides), black poplar (populus nigra), and american elm (ulmus americana) contained high numbers of aerobic and anaerobic pectin-degrading bacteria (10 to 10 cells per g of wood). high activity of polygalacturonate lyase (</=0.5 u/ml) was also detected in the fetid liquid that spurted from wetwood zones in the lower trunk when the trees were bored. a prevalent pectin-degrading obligately anaerobic bacterium isolated from these ...198116345848
efficient agrobacterium-mediated transformation of commercial hybrid poplar populus nigra l. x p. maximowiczii a. henry.many economically important species of populus, especially those in sections aigeiros and tacamahaca, remain recalcitrant to genetic transformation. in this study, a simple and reliable protocol was developed for the efficient agrobacterium-mediated transformation of a difficult-to-transform, but commercially viable, hybrid poplar populus nigra l. x p. maximowiczii a. henry (nm6). a plant transformation vector designed to express the beta-glucuronidase (gus) gene was used to detect transformatio ...201020087597
the role of timing, duration, and frequency of inundation in controlling leaf litter decomposition in a river-floodplain ecosystem (tagliamento, northeastern italy).despite growing recognition of the importance of a natural flow regime in river-floodplain systems, researchers struggle to quantify ecosystem responses to altered hydrological regimes. how do frequency, timing, and duration of inundation affect fundamental ecosystem processes such as leaf litter decomposition? along the semi-natural tagliamento river corridor, located in northeastern italy, we employed in situ experiments to separate effects of different inundation components on breakdown rates ...200616237537
differential assimilation of nitrogen dioxide by 70 taxa of roadside trees at an urban pollution level.in order to screen for the best species for mitigating nitrogen dioxide (no2) by plants at urban levels, we investigated assimilation of nitrogen dioxide by 70 taxa of woody plants that are mostly utilized as roadside trees. they were fumigated with 15n-labeled no2 at 0.1 microl l(-1) for 8h, and the amount of reduced nitrogen derived from no2 (in mg ng(-1) dry weight) in the leaves (designated no2 assimilation capability hereafter) were determined. data were analyzed in the comparison with the ...200516219499
ligand loop effects on the free energy change of redox and ph-dependent equilibria in cupredoxins probed on amicyanin variants.in this work, we have determined the thermodynamic parameters of the reduction of four different variants of thiobacillus versutus amicyanin by electrochemical techniques. in addition, the thermodynamic parameters were determined of the low-ph conformational change involving protonation of the c-terminal histidine ligand and the concomitant dissociation of this histidine from the cu(i) ion. in these variants, the native c-terminal loop containing the cys, his, and met copper ligands has been rep ...200516026167
antimicrobial flavonoids from the twigs of populus nigra × populus deltoides.a bioassay-guided fractionation of the ethyl acetate extract from the twigs of the hybrid poplar 'neva', populus nigra l. × populus deltoides marsh, led to the isolation of three flavonoids, which were identified by means of spectrometric and physicochemical analysis as 5-hydroxy-7-methoxy-flavone (1), 5,7-dihydoxy-flavone (2) and 5,7-dihydroxy-flavonol (3). these compounds were further screened for their antimicrobial activity against plant pathogens, including three bacteria (pseudomonas lachr ...201121416454
[sensitization to castanea sativa pollen and pollinosis in northern extremadura (spain)].castanea sativa pollen allergy has generally been considered to be uncommon and clinically insignificant. in our geographical area (plasencia, cáceres, spain) castanea sativa pollen is a major pollen.200515946627
host-associated populations in the lettuce root aphid, pemphigus bursarius (l.).pemphigus bursarius is a host-alternating aphid in which annual rounds of sexual reproduction on its primary host, populus nigra, are interspersed with parthenogenesis on a range of secondary hosts. evidence was sought for the existence of genetically distinct populations, associated with different secondary hosts, in p. bursarius. microsatellite markers revealed that genetically distinct populations were present on three different secondary host species. microsatellites were also used, in conju ...200515785781
laboratory-scale measurements of n2o and ch4 emissions from hybrid poplars (populus deltoides x populus nigra).the purpose of this study was to determine whether or not young hybrid poplar (populus deltoides x populus nigra) could transport landfill biogas internally from the root zone to the atmosphere, thereby acting as conduits for landfill gas release. fluxes of methane (ch4) and nitrous oxide (n2o) from the seedlings to the atmosphere were measured under controlled conditions using dynamic flux chambers and a tunable diode laser trace gas analyser (tdltga). nitrous oxide was emitted from the seedlin ...200415666448
hybrid poplar and forest soil response to municipal and industrial by-products: a greenhouse study.little research has been conducted in the lake states (minnesota, wisconsin, and michigan) to evaluate the effects of municipal and industrial by-product applications on the early growth of short rotation woody crops such as hybrid poplar. anticipated shortages of harvestable-age aspen in the next decade can be alleviated and rural development can be enhanced through the application of by-products to forest soils. this study was conducted to evaluate the effects of inorganic fertilizer, boiler a ...200415224944
molecular analysis of natural populations of populus nigra l intermingled with cultivated hybrids.in this study six simple sequence repeats (ssr or microsatellites) were selected for their ability to fingerprint a total of 60 commercial clones of populus deltoides marsh. and populus x canadensis moench (typically derived from crosses between populus nigra l and p. deltoides) and to characterize a natural population of p. nigra growing along the ticino river in the north of italy. out of six ssrs used, four microsatellite loci were found to have alleles which were species-specific to p. delto ...200312859627
ozone air pollution and foliar injury development on native plants of switzerland.the objectives of this study were to examine the foliar sensitivity to ozone exposure of 12 tree, shrub, and herbaceous species native to southern switzerland and determine the seasonal cumulative ozone exposures required to induce visible foliar injury. the study was conducted from the beginning of may through the end of august during 2000 and 2001 using an open-top chamber research facility located within the lattecaldo cantonal forest nursery in canton ticino, southern switzerland (600 m asl) ...200312804826
native venturia inopina sp. nov., specific to populus trichocarpa and its hybrids.venturia populina, first described on european populus nigra, has been thought to be the only species of venturia in europe and north america to cause leaf and shoot blight of balsam poplars and cottonwoods in populus sects. tacamahaca and aigeiros. the species of venturia occurring on introduced p. nigra and native p. trichocarpa in the pacific northwest were examined. venturia populina was consistently found on p. nigra (i.e. the widespread p. nigra cv. 'italica') in the region, but v. inopina ...200312735251
pathogenicity of beauveria bassiana (deuteromycotina: hypomycetes) to larvae of the small poplar longhorn beetle, saperda populnea (coleoptera: cerambycidae).the small poplar longhorn beetle, saperda populnea is an important pest of lombardy poplars (populus nigra l.) in turkey. a survey for natural entomopathogenic fungi of s. populnea larvae was made in erzurum, turkey, during the period 2004-2005. larvae (13.5%) infected with a strain of the fungus beauveria bassiana were found. the pathogenicity of b. bassiana strain 46 was conducted with different concentrations of conidia (10(6), 10(7) and 10(8) conidia/ml) of this isolate on s. populnea larvae ...200616830195
genome-wide analysis of a talea-introduced transgenic populus simonii × populus nigra dwarf mutant.a dwarf mutant (dwf1) was obtained among 15 transgenic lines, when talea (tamarix androssowii late embryogenesis abundant gene) was introduced into populus simonii × populus nigra by agrobacterium tumefaciens-mediated transformation. under the same growth conditions, dwf1 height was significantly reduced compared with the wild type and the other transgenic lines. because only one transgenic line (dwf1) displayed the dwarf phenotype, we considered that t-dna insertion sites may play a role in the ...201222489122
testing applicability of black poplar (populus nigra l.) bark to heavy metal air pollution monitoring in urban and industrial regions.a comparative study of the capabilities of black poplar-tree (populus nigra l.) bark as a biomonitor of atmospheric heavy-metal pollution is reported. performance indicators (concentrations and enrichment factors) of heavy metal bioaccumulation of bark were compared to the corresponding indicators of epiphytic lichens xanthoria parietina (l.) th. fr. and physcia adscendens (fr.) h. oliver, collected simultaneously with bark samples within the kiev urban-industrial conurbation. the concentrations ...200717140640
stomatal uptake and stomatal deposition of ozone in isoprene and monoterpene emitting plants.volatile isoprenoids were reported to protect plants against ozone. to understand whether this could be the result of a direct scavenging of ozone by these molecules, the stomatal and non-stomatal uptake of ozone was estimated in plants emitting isoprene or monoterpenes. ozone uptake by holm oak (quercus ilex, a monoterpene emitter) and black poplar (populus nigra, an isoprene emitter) was studied in whole plant enclosures (continuously stirred tank reactors, cstr). the ozone uptake by plants wa ...200817538866
bioprofiling of salicaceae bud extracts through high-performance thin-layer chromatography hyphenated to biochemical, microbiological and chemical detections.the buds of poplars (populus l.) and willows (salix l.), both from the same family (salicaceae mirbel), are increasingly used in gemmotherapy and importantly contribute to the production of the physiologically active propolis by european bee apis mellifera l. in order to study their phenolic profiles, polar extracts of buds from p. nigra l. were compared to those of p. alba l. and s. alba l. through high-performance thin-layer chromatography (hptlc). five chemotypical patterns were distinguished ...201728222858
arbuscular mycorrhizal fungi differentially affect the response to high zinc concentrations of two registered poplar clones.the effects of a high concentration of zinc on two registered clones of poplar (populus alba villafranca and populus nigra jean pourtet), inoculated or not with two arbuscular mycorrhizal fungi (glomus mosseae or glomus intraradices) before transplanting them into polluted soil, were investigated, with special regard to the extent of root colonization by the fungi, plant growth, metal accumulation in the different plant organs, and leaf polyamine concentration. zinc accumulation was lower in jea ...200817888550
thresholds of heavy-metal toxicity in cuttings of european black poplar (populus nigra l.) determined according to antioxidant status of fine roots and morphometrical disorders.the effects of increasing concentrations of polluted soils collected from two different stands in the direct neighbourhood of a copper smelter were studied by analyzing the activity of lipid peroxidation and antioxidant enzymes in the fine roots of cuttings of black poplar (populus nigra l.). morphological parameters of affected and unaffected plants were compared and concentrations of heavy metals in their fine roots were estimated. copper and lead were the major pollutants, and their threshold ...200817963819
[application of populus nigra preparations at experimental parodontitis].severe oxidative stress, developed under experimental periodontitis is accompanied by disturbances in mitochondrial respiration in tissue cells of gingiva, membrane damage and release of fe(2+) and mn(2+), leading to the worsening of inflammation process and gingival tissue necrosis. reduction of free nitric oxide in gingival tissue appeared to be characteristic for experimental parodontitis: decreases local immunity, antimicrobial resistance, and tissue regeneration, disturbs blood supply and t ...200717984561
vegetative buffers for fan emissions from poultry farms: 1. temperature and foliar nitrogen.this study sought to evaluate the potential of trees planted around commercial poultry farms to trap ammonia (nh(3)), the gas of greatest environmental concern to the poultry industry. four plant species (norway spruce, spike hybrid poplar, streamco willow, and hybrid willow) were planted on eight commercial farms from 2003 to 2004. because temperature (t) can be a stressor for trees, t was monitored in 2005 with data loggers among the trees in front of the exhaust fans (11.4 to 17.7 m) and at a ...200818246513
genetic structure of the poplar rust fungus melampsora larici-populina: evidence for isolation by distance in europe and recent founder effects overseas.dispersal has a great impact on the genetic structure of populations, but remains difficult to estimate by direct measures. in particular, gradual and stochastic dispersal are often difficult to assess and to distinguish, although they have different evolutionary consequences. plant pathogens, especially rust fungi, are suspected to display both dispersal modes, though on different spatial scales. in this study, we inferred dispersal capacities of the poplar rust fungus melampsora larici-populin ...200818499532
in situ investigation of leaf water status by portable unilateral nuclear magnetic resonance.a portable unilateral nuclear magnetic resonance (nmr) instrument was used to detect in field conditions the water status of leaves of herbaceous crops (zea mays, phaseolus vulgaris), mesophyllous trees (populus nigra), and natural mediterranean vegetation characterized by water-spending shrubs (cistus incanus) and water-saving sclerophyllous trees (quercus ilex). a good relationship was observed between nmr signal, leaf relative water content, and leaf transpiration in herbaceous leaves undergo ...200919193862
transgenic manipulation of a single polyamine in poplar cells affects the accumulation of all amino acids.the polyamine metabolic pathway is intricately connected to metabolism of several amino acids. while ornithine and arginine are direct precursors of putrescine, they themselves are synthesized from glutamate in multiple steps involving several enzymes. additionally, glutamate is an amino group donor for several other amino acids and acts as a substrate for biosynthesis of proline and gamma-aminobutyric acid, metabolites that play important roles in plant development and stress response. suspensi ...201019649694
productivity, water-use efficiency and tolerance to moderate water deficit correlate in 33 poplar genotypes from a populus deltoides x populus trichocarpa f1 progeny.genotypic variability for productivity, water-use efficiency and leaf traits in 33 genotypes selected from an f1 progeny of populus deltoides bartr. ex marsh x populus trichocarpa l. was explored under optimal and moderate water-deficit conditions. saplings of the 33 genotypes were grown in a two-plot open field at inra orléans (france) and coppiced every year. a moderate water deficit was induced during two successive years on one plot by withholding irrigation, while the second one remained ir ...200919773340
repeated unidirectional introgression towards populus balsamifera in contact zones of exotic and native poplars.as the evolutionary significance of hybridization is largely dictated by its extent beyond the first generation, we broadly surveyed patterns of introgression across a sympatric zone of two native poplars (populus balsamifera, populus deltoides) in quebec, canada within which european exotic populus nigra and its hybrids have been extensively planted since the 1800s. single nucleotide polymorphisms (snps) that appeared fixed within each species were characterized by dna-sequencing pools of pure ...201020002578
selective behaviour of honeybees in acquiring european propolis plant precursors.honey bees harvest resins from various plant species and use them in the hive as propolis. while there have been a number of studies concerning the chemical composition of this antimicrobial product, little is known about selective behavior and bee preference when different potential plant sources of resin are available. the main objective of this paper was to investigate some aspects of behavioral patterns of honeybees in the context of resin acquisition. samples of propolis originating from te ...201627294416
the response of high and low polyamine-producing cell lines to aluminum and calcium stress.the diamine putrescine (put) has been shown to accumulate in tree leaves in response to high al and low ca in the soil, leading to the suggestion that this response may provide a physiological advantage to leaf cells under conditions of al stress. the increase in put is reversed by ca supplementation in the soil. using two cell lines of poplar (populus nigra x maximowiczii), one with constitutively high put (resulting from transgenic expression of a mouse ornithine decarboxylase--called hp cells ...201020552726
use of atomic absorption spectrometry in assessment of biomonitor plants for lead, cadmium and copper pollution.eleven plant species were collected from the vicinity of lead-battery plant in the city of gaziantep, turkey. lead, cadmium and copper concentrations in the soil and leaves of plants were determined by atomic absorption spectrometry. lead, cd and cu concentrations in the soil samples taken from battery area were found to be in the ranges of 304-602, 0.4-0.44 and 31-37 mg x kg(-1), respectively. significantly increased lead concentration up to 2 750 mg x kg(-1) was found in the leaves of eleagnus ...201222497165
xanthine oxidase inhibitory properties of czech medicinal plants.to investigate in vitro xanthine oxidase inhibitory properties of plants traditionally used in czech republic and central-east europe region for gout, arthritis or rheumatism treatment.201020800669
living with high putrescine: expression of ornithine and arginine biosynthetic pathway genes in high and low putrescine producing poplar cells.arginine (arg) and ornithine (orn), both derived from glutamate (glu), are the primary substrates for polyamine (pa) biosynthesis, and also play important roles as substrates and intermediates of overall n metabolism in plants. their cellular homeostasis is subject to multiple levels of regulation. using reverse transcription quantitative pcr (rt-qpcr), we studied changes in the expression of all genes of the orn/arg biosynthetic pathway in response to up-regulation [via transgenic expression of ...201021082203
phenolic composition and antioxidant properties of poplar bud (populus nigra) extract: individual antioxidant contribution of phenolics and transcriptional effect on skin aging.the populus species possess great potential for therapeutical applications, especially for their known anti-inflammatory properties. the antioxidant properties of propolis, a hive product collected by honey bees mainly from poplar bud exudates, suggest that poplar buds also possess antioxidant properties. here is reported the characterization of the antioxidant properties of an aqueous poplar bud (populus nigra) extract. it presented a high total phenolic content, and moderate antioxidant proper ...201121425781
biosynthesis of isoprene in escherichia coli via methylerythritol phosphate (mep) pathway.isoprene is an aviation fuel of high quality and an important polymer building block in the synthetic chemistry industry. in light of high oil prices, sustained availability, and environmental concerns, isoprene from renewable materials is contemplated as a substitute for petroleum-based product. escherichia coli with advantages over other wild microorganisms, is considered as a powerful host for biofuels and chemicals. here, we constructed a synthetic pathway of isoprene in e. coli by introduci ...201121468716
leaf litter leachates have the potential to increase lifespan, body size, and offspring numbers in a clone of moina macrocopa.leaf litter processing is one major pathway of the global organic carbon cycle. during this process, a variety of small reactive organic compounds are released and transported to the aquatic environment, and may directly impact aquatic organisms as natural xenobiotics. we hypothesize that different forest stockings produce different leachate qualities, which in turn, stress the aquatic communities and, eventually, separate sensitive from tolerant species. particularly, leachates from coniferous ...201122115468
[Effects of cadmium stress on the microbial biodiversity in purple soil and alluvial soil potted with a poplar (Populus deltoides x Populus nigra)].Effects of current Cd contamination levels on microbial biodiversity were studied under the typical Cd contaminated soils in the Yangtze Basin. Purple soil and alluvial soil potted with a poplar (Populus deltoides x Populus nigra) were selected, and the culturable soil microbial amounts by flat method, microbial biomass and bacterial community structure by PCR-DGGE were investigated. Cd supplies significantly increased the culturable amounts of bacteria and actinomyces in purple soil, but decrea ...201121922843
[ectopic expression of the pnantl1 and pnantl2 black poplar genes in transgenic tobacco plants].four putative orthologs of the aintegumenta gene were found in the poplar (populus trichocarpa) genome. two of them, which we called pnantl1 and pnantl2, were isolated from black poplar (populus nigra l.) and transgenic tobacco plants were generated on their basis. tobacco plants that were transgenic for the pnantl1 gene were characterized by increased leaf length, smaller flower size, and different defects in the development of the corolla and stamens. tobacco plants that were transgenic for th ...201223270264
exploring the microbiota dynamics related to vegetable biomasses degradation and study of lignocellulose-degrading bacteria for industrial biotechnological application.the aims of this study were to evaluate the microbial diversity of different lignocellulosic biomasses during degradation under natural conditions and to isolate, select, characterise new well-adapted bacterial strains to detect potentially improved enzyme-producing bacteria. the microbiota of biomass piles of arundo donax, eucalyptus camaldulensis and populus nigra were evaluated by high-throughput sequencing. a highly complex bacterial community was found, composed of ubiquitous bacteria, with ...201525641069
better to light a candle than curse the darkness: illuminating spatial localization and temporal dynamics of rapid microbial growth in the rhizosphere.the rhizosphere is a hotbed of microbial activity in ecosystems, fueled by carbon compounds from plant roots. basic questions about the location and dynamics of plant-spurred microbial growth in the rhizosphere are difficult to answer with standard, destructive soil assays mixing a multitude of microbe-scale microenvironments in a single, often sieved, sample. soil microbial biosensors designed with the luxcdabe reporter genes fused to a promoter of interest enable continuous imaging of the micr ...201324032034
qtls for woolly poplar aphid (phloeomyzus passerinii l.) resistance detected in an inter-specific populus deltoides x p. nigra mapping population.the genus populus represents one of the most economically important groups of forest trees. it is composed by approximately 30 species used for wood and non-wood products, phytoremediation and biomass. poplar is subjected to several biological and environmental threats although, compared to annual crops, we know far less about the genetic bases of biotic stress resistance. woolly poplar aphid (phloeomyzus passerinii) is considered a main pest of cultivated poplars in european and american countr ...201627022954
a label-free differential quantitative proteomics analysis of a talea-introduced transgenic populus simonii × populus nigra dwarf mutant.talea (tamarix androssowii late embryogenesis abundant gene, dq663481) gene was introduced into populus simonii × populus nigra by agrobacterium tumefaciens-mediated transformation with the aim of improving salt-tolerance. among the 15 transgenic lines, one showed a dwarf phenotype (dwf1). under the same growth conditions, dwf1 height was significantly reduced compared with the wild-type and the other transgenic lines. the mechanisms underlying this effect were investigated in mutant and wild-ty ...201222327784
genetic transformation of populus nigra by agrobacterium tumefaciens.two clones of populus nigra l. were tested in vivo and in vitro for their susceptibility to three different agrobacterium tumefaciens wild-type strains evaluating number and size of resulting calluses. strain c58 proved to be the most virulent.various parameters affecting agrobacterium-mediated transformation of p. nigra clones were further analyzed using ß-glucuronidase gene transient expression. the clone jean pourtet proved to be more susceptible than the clone san giorgio. a. tumefaciens str ...199424193760
phylogenetic and stress-responsive expression analysis of 20 wrky genes in populus simonii × populus nigra.wrky transcription factors play important roles in regulating biotic and abiotic stress responses in plants. although a plethora of studies have revealed the functions and mechanisms of some wrkys in various plants, the studies of wrkys in woody plants especially tree species under different abiotic and biotic stress conditions are still not well characterized. in this study, we selected 20 populus simonii×populus nigra wrky genes based on our previous transcriptome study, and characterized thes ...201525843624
an empirical assessment of transgene flow from a bt transgenic poplar plantation.to assess the possible impact of transgenic poplar plantations on the ecosystem, we analyzed the frequency and distance of gene flow from a mature male transgenic populus nigra plantation carrying the bacillus thuringiensis toxin gene (bt poplar) and the survival of bt poplar seeds. the resultant bt poplar seeds occurred at a frequency of ~0.15% at 0 m to ~0.02% at 500 m from the bt poplar plantation. the germination of bt poplar seeds diminished within three weeks in the field (germination rate ...201728085955
isolation and functional characterization of jcft, a flowering locus t (ft) homologous gene from the biofuel plant jatropha curcas.physic nut (jatropha curcas l.) is a potential feedstock for biofuel production because jatropha oil is highly suitable for the production of the biodiesel and bio-jet fuels. however, jatropha exhibits low seed yield as a result of unreliable and poor flowering. flowering locus t (ft) -like genes are important flowering regulators in higher plants. to date, the flowering genes in jatropha have not yet been identified or characterized.201424886195
populus simonii × populus nigra wrky70 is involved in salt stress and leaf blight disease responses.wrky transcription factors (tfs) are important regulators in the complex stress response signaling networks in plants, but the detailed mechanisms underlying these regulatory networks have not been fully characterized. in the present study, we identified a group iii wrky gene (psnwrky70, potri.016g137900) from populussimonii × populusnigra and explored its function under salt and pathogen stresses. the promoter sequence that is located 2471-bp upstream from the start codon (sc) of psnwrky70 cont ...201728369503
validation of the stomatal flux approach for the assessment of ozone visible injury in young forest trees. results from the top (transboundary ozone pollution) experiment at curno, italy.this paper summarises some of the main results of a two-year experiment carried out in an open-top chambers facility in northern italy. seedlings of populus nigra, fagus sylvatica, quercus robur and fraxinus excelsior have been subjected to different ozone treatments (charcoal-filtered and non-filtered air) and soil moisture regimes (irrigated and non-irrigated plots). stomatal conductance models were applied and parameterised under south alpine environmental conditions and stomatal ozone fluxes ...200919019512
genome survey and characterization of endophytic bacteria exhibiting a beneficial effect on growth and development of poplar trees.the association of endophytic bacteria with their plant hosts has a beneficial effect for many different plant species. our goal is to identify endophytic bacteria that improve the biomass production and the carbon sequestration potential of poplar trees (populus spp.) when grown in marginal soil and to gain an insight in the mechanisms underlying plant growth promotion. members of the gammaproteobacteria dominated a collection of 78 bacterial endophytes isolated from poplar and willow trees. as ...200919060168
mapped dna probes from loblolly pine can be used for restriction fragment length polymorphism mapping in other conifers.a high-density genetic map based on restriction fragment length polymorphisms (rflps) is being constructed for loblolly pine (pinus taeda l.). consequently, a large number of dna probes from loblolly pine are potentially available for use in other species. we have used some of these dna probes to detect rflps in 12 conifers and an angiosperm. thirty complementary dna and two genomic dna probes from loblolly pine were hybridized to southern blots containing dna from five species of pinus (p. elli ...199424186006
preparation and characterisation of biodegradable pollen-chitosan microcapsules and its application in heavy metal removal.biosorbents have been widely used in heavy metal removal. new resources should be exploited to develop more efficient biosorbents. this study reports the preparation of three novel chitosan microcapsules from pollens of three common, wind-pollinated plants (acer negundo, cupressus sempervirens and populus nigra). the microcapsules were characterized (fourier transform infrared spectroscopy, thermogravimetric analysis, scanning electron microscopy and elemental analysis) and used in removal of he ...201525479387
chlorophyll fluorescence kinetics, photosynthetic activity, and pigment composition of blue-shade and half-shade leaves as compared to sun and shade leaves of different trees.the chlorophyll (chl) fluorescence induction kinetics, net photosynthetic co2 fixation rates p n, and composition of photosynthetic pigments of differently light exposed leaves of several trees were comparatively measured to determine the differences in photosynthetic activity and pigment adaptation of leaves. the functional measurements were carried out with sun, half-shade and shade leaves of seven different trees species. these were: acer platanoides l., ginkgo biloba l., fagus sylvatica l., ...201323670216
aided phytostabilisation reduces metal toxicity, improves soil fertility and enhances microbial activity in cu-rich mine tailings.(aided) phytostabilisation has been proposed as a suitable technique to decrease the environmental risks associated with metal(loid)-enriched mine tailings. field scale evaluations are needed for demonstrating their effectiveness in the medium- to long-term. a field trial was implemented in spring 2011 in cu-rich mine tailings in the nw of spain. the tailings were amended with composted municipal solid wastes and planted with salix spp., populus nigra l. or agrostis capillaris l. cv. highland. p ...201727817970
leaf mineral nutrient remobilization during leaf senescence and modulation by nutrient deficiency.higher plants have to cope with fluctuating mineral resource availability. however, strategies such as stimulation of root growth, increased transporter activities, and nutrient storage and remobilization have been mostly studied for only a few macronutrients. leaves of cultivated crops (zea mays, brassica napus, pisum sativum, triticum aestivum, hordeum vulgare) and tree species (quercus robur, populus nigra, alnus glutinosa) grown under field conditions were harvested regularly during their li ...201526029223
effects of the fungicide tebuconazole on microbial capacities for litter breakdown in streams.streams draining agricultural basins are subjected to the input of fungicides which can affect aquatic microbial communities. we analyzed the effect of the fungicide tebuconazole (tbz) on alnus glutinosa and populus nigra litter breakdown by aquatic microorganisms. for six weeks, fungal and bacterial responses were analyzed in indoor stream channels subjected to tbz-contaminated (33.1±12.4 μg l(-1)) and uncontaminated conditions. litter breakdown rates decreased in presence of tbz. the decrease ...201222824240
greenhouse gas emissions from a cu-contaminated soil remediated by in situ stabilization and phytomanaged by a mixed stand of poplar, willows and false indigo-bush.phytomanagement of trace element-contaminated soils can reduce the soil toxicity and restore the soil ecological functions, including the soil gas exchange with the atmosphere. we studied the emission rate of the greenhouse gases (ghg) co2, ch4 and n2o, and the potential ch4 oxidation, denitrification activity (dea) and glucose mineralization of a cu-contaminated soil amended with dolomitic limestone and compost, alone or in combination, after a 2 year-phytomanagement with a mixed stand of popul ...201728165773
identification of a hydrolyzable tannin, oenothein b, as an aluminum-detoxifying ligand in a highly aluminum-resistant tree, eucalyptus camaldulensis.eucalyptus camaldulensis is a tree species in the myrtaceae that exhibits extremely high resistance to aluminum (al). to explore a novel mechanism of al resistance in plants, we examined the al-binding ligands in roots and their role in al resistance of e. camaldulensis. we identified a novel type of al-binding ligand, oenothein b, which is a dimeric hydrolyzable tannin with many adjacent phenolic hydroxyl groups. oenothein b was isolated from root extracts of e. camaldulensis by reverse-phase h ...201424381064
putrescine overproduction does not affect the catabolism of spermidine and spermine in poplar and arabidopsis.the effect of up-regulation of putrescine (put) production by genetic manipulation on the turnover of spermidine (spd) and spermine (spm) was investigated in transgenic cells of poplar (populus nigra × maximowiczii) and seedlings of arabidopsis thaliana. several-fold increase in put production was achieved by expressing a mouse ornithine decarboxylase cdna either under the control of a constitutive (in poplar) or an inducible (in arabidopsis) promoter. the transgenic poplar cells produced and ac ...201424013280
do symplasmic networks in cambial zones correspond with secondary growth patterns?the plasmodesmal (pd) network in the cambial zone of arabidopsis thaliana hypocotyls was analysed using electron microscopy and dye-coupling studies and compared to those of internodes of populus nigra and solanum lycopersicum. in all species, pd densities and frequencies undergo alterations in topologically successive cambial walls reflecting species-specific patterns of pd degradation and pd insertion during cell development. longitudinal pd fission is responsible for an abrupt increment of pd ...201120853011
boron accumulation and toxicity in hybrid poplar (populus nigra × euramericana).poplars accumulate high b concentrations and are thus used for the phytomanagement of b contaminated soils. here, we performed pot experiments in which populus nigra × euramericana were grown on a substrate with b concentrations ranging from 13 to 280 mg kg(-1) as h(3)bo(3). salix viminalis, brassica juncea, and lupinus albus were grown under some growing conditions for comparison. poplar growth was unaffected at soil b treatment levels up to 93 mg kg(-1). growth was progressively reduced at lev ...201122050628
manipulating beaver (castor canadensis) feeding responses to invasive tamarisk (tamarix spp.).to evaluate methods for promoting consumption of tamarisk plants by beavers (castor canadensis), we determined the feeding responses by captive beavers to diets that contained tannins and sodium chloride (hereafter referred to as tamarisk diet). in two-choice tests, beavers consumed equivalent quantities of tamarisk diet and control diet. treatment with polyethylene glycol and fructose did not increase beaver preferences for the tamarisk diet. when offered the choice of control diet and casein h ...200818581180
metal concentrations in the soils and native plants surrounding the old flotation tailings pond of the copper mining and smelting complex bor (serbia).in this study concentrations of metals in the native plants and soils surrounding the old flotation tailings pond of the copper mine were determined. it has been established that the soil is heavily contaminated with copper, iron and arsenic, the mean concentrations being 1585.6, 29,462.5 and 171.7 mg kg(-1) respectively. all the plants, except manganese, accumulated metallic elements in concentrations which were either in the range of critical and phytotoxic values (pb and as) or higher (zn), a ...201222314513
influence of poplar clones on fertility life-table parameters of chaitophorus leucomelas (hemiptera: aphididae).the aphid chaitophorus leucomelas koch (hemiptera: aphididae) is one of the most important pests of poplar (populus spp.) plantations in iran. in this study, development, reproduction, and life history of the aphid were assessed on 11 poplar clones; belong to three species, populus nigra l., populus deltoides bartram ex marshall, and populus. euramericana guinier. the experiments were carried out under controlled conditions at 24 +/- 1 degrees c, 50-60% rh, and a photoperiod of 12:12 (l:d) h. th ...201021309247
a real-scale soil phytoremediation.in the present investigation, a phytoremediation process with a combination of different plant species (populus nigra (var.italica), paulownia tomentosa and cytisus scoparius), and natural growing vegetation has been proposed at real-scale (10.000 m(2)) to bioremediate and functionally recover a soil historically contaminated by heavy metals and hydrocarbons. in the attempts to assess both effectiveness and evolution of the remediation system towards a natural soil ecosystem, besides the polluti ...201323179352
consequences of warming and resource quality on the stoichiometry and nutrient cycling of a stream shredder.as a result of climate change, streams are warming and their runoff has been decreasing in most temperate areas. these changes can affect consumers directly by increasing their metabolic rates and modifying their physiology and indirectly by changing the quality of the resources on which organisms depend. in this study, a common stream detritivore (echinogammarus berilloni catta) was reared at two temperatures (15 and 20°c) and fed populus nigra l. leaves that had been conditioned either in an i ...201525738818
discovery of genes coding for carbohydrate-active enzyme by metagenomic analysis of lignocellulosic biomasses.in this study, a high-throughput sequencing approach was applied to discover novel biocatalysts for lignocellulose hydrolysis from three dedicated energy crops, arundo donax, eucalyptus camaldulensis and populus nigra, after natural biodegradation. the microbiomes of the three lignocellulosic biomasses were dominated by bacterial species (approximately 90%) with the highest representation by the streptomyces genus both in the total microbial community composition and in the microbial diversity r ...201728198423
effects of ozone exposure in open-top chambers on poplar (populus nigra) and beech (fagus sylvatica): a comparison.rooted cuttings of poplar (populus nigra) and seedlings of beech (fagus sylvatica) were exposed to ozone in open-top chambers for one growing season. three treatments were applied: charcoal-filtered (cf), non-filtered (nf) and non-filtered air plus 30 ppb (nl l(-1)) ozone (nf+). extra ozone was only added on clear days, from 09:00 until 17:00-20:00. the aot40s (accumulated exposure over a threshold of 40 ppb), calculated from april to september were 4055 ppb.h for the nf and 8880 ppb.h for the n ...200015092884
ozone air pollution effects on tree-ring growth, delta(13)c, visible foliar injury and leaf gas exchange in three ozone-sensitive woody plant species.we assessed the effects of ambient tropospheric ozone on annual tree-ring growth, delta(13)c in the rings, leaf gas exchange and visible injury in three ozone-sensitive woody plant species in southern switzerland. seedlings of populus nigra l., viburnum lantana l. and fraxinus excelsior l. were exposed to charcoal-filtered air (cf) and non-filtered air (nf) in open-top chambers, and to ambient air (aa) in open plots during the 2001 and 2002 growing seasons. ambient ozone exposures in the region ...200717403646
seasonal trends in reduced leaf gas exchange and ozone-induced foliar injury in three ozone sensitive woody plant species.seasonal trends in leaf gas exchange and ozone-induced visible foliar injury were investigated for three ozone sensitive woody plant species. seedlings of populus nigra l., viburnum lantana l., and fraxinus excelsior l. were grown in charcoal-filtered chambers, non-filtered chambers and open plots. injury assessments and leaf gas exchange measurements were conducted from june to october during 2002. all species developed typical ozone-induced foliar injury. for plants exposed to non-filtered air ...200515809106
lignocellulose-adapted endo-cellulase producing streptomyces strains for bioconversion of cellulose-based materials.twenty-four actinobacteria strains, isolated from arundo donax, eucalyptus camaldulensis and populus nigra biomass during natural biodegradation and with potential enzymatic activities specific for the degradation of lignocellulosic materials, were identified by a polyphasic approach. all strains belonged to the genus streptomyces (s.) and in particular, the most highly represented species was streptomyces argenteolus representing 50% of strains, while 8 strains were identified as streptomyces f ...201628066379
fungal planet description sheets: 320-370.novel species of fungi described in the present study include the following from malaysia: castanediella eucalypti from eucalyptus pellita, codinaea acacia from acacia mangium, emarcea eucalyptigena from eucalyptus brassiana, myrtapenidiella eucalyptorum from eucalyptus pellita, pilidiella eucalyptigena from eucalyptus brassiana and strelitziana malaysiana from acacia mangium. furthermore, stachybotrys sansevieriicola is described from sansevieria ehrenbergii (tanzania), phacidium grevilleae fro ...201526240451
correlation between the carbon isotope discrimination in leaf starch and sugars of c(3) plants and the ratio of intercellular and atmospheric partial pressures of carbon dioxide.carbon isotope discrimination (delta) was analyzed in leaf starch and soluble sugars, which represent most of the recently fixed carbon. plants of three c(3) species (populus nigra l. x p. deltoides marsh., gossypium hirsutum l. and phaseolus vulgaris l.) were kept in the dark for 24 hours to decrease contents of starch and sugar in leaves. then gas exchange measurements were made with constant conditions for 8 hours, and subsequently starch and soluble sugars were extracted for analysis of carb ...198816666476
selection of woody species for wastewater enhancement and restoration of riparian woodlands.growth and nutrient uptake of seven tree species were evaluated with the goal of selecting the species that can be used for wastewater enhancement by dendro-purification, or green tree filtering, and for restoration of riparian woodlands. trees were grown in pots with an inert mixture of perlite and vermiculite and irrigated with either nutrient solution or treated wastewater we measured the effects of species and irrigation water on biomass and nutrient content of leaves, stems and roots. for m ...200818972692
effect of environmental stress factors on ecophysiological traits and susceptibility to pathogens of five populus clones throughout the growing season.the variability of ecophysiological traits associated with productivity (e.g., water relations, leaf structure, photosynthesis and nitrogen (n) content) and susceptibility to fungal and insect infection were investigated in five poplar clones (populus deltoides batr.-lux clone; populus nigra l.-58-861 clone and populus × canadensis mönch.-luisa avanzo, i-214 and adige clones) during their growing season. the objective of the study was to determine their physiological responses under summer const ...201323824242
molecular cloning and characterization of ghlecrk, a novel kinase gene with lectin-like domain from gossypium hirsutum.a novel gene encoding a lectin-like protein kinase was cloned from the upland cotton (gossypium hirsutum) through cdna library screening. this gene (named as ghlecrk; genbank accession number: ay487461) had a total length of 2233bp with an open reading frame of 1926bp, and encoded a predicted polypeptide of 641 amino acids with a molecular weight of 71.16kda. the ghlecrk protein shared 73, 65, 64 and 59% identity with other lectin-like kinase proteins isolated from a. thaliana (at3g53810, at2g37 ...200415354356
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