| [pharmacological studies on the saponin isolated from the seed of momordica cochinchinensis sprenger]. | | 1971 | 5102345 |
| production and characterization of monoclonal antibodies against the ribosome inactivating proteins dianthin32 and momochin. | female balb/c mice were immunized with either dianthin32 or momochin, type 1 ribosome-inactivating proteins (rips) derived from dianthus charyophyllus and momordica cochinchinensis, respectively. five anti-dianthin32 and 6 anti-momochin secreting hybridomas were obtained by somatic fusion of lymphocytes with myeloma cell line ns0. the monoclonal antibodies (mabs) produced were highly specific, as demonstrated by cross-reactivity assays performed with taxonomically related and unrelated type 1 ri ... | 1994 | 7519581 |
| enhancement of serological immune responses to foot-and-mouth disease vaccine by a supplement made of extract of cochinchina momordica seeds. | foot-and-mouth disease (fmd) is a highly contagious disease affecting cloven-hoofed animals. vaccination against fmd is a routine practice in many countries where the disease is endemic. this study was designed first to investigate the extract of the seeds of momordica cochinchinensis (lour.) spreng. (ecms) for its adjuvant effect on vaccination of inactivated fmdv antigens in a guinea pig model and then to evaluate the supplement of ecms in oil-emulsified fmd vaccines for its immunopotentiation ... | 2007 | 17942610 |
| enhancement of gastric ulcer healing and angiogenesis by cochinchina momordica seed extract in rats. | cochinchina momordica seed is the dried ripe seed of momordica cochinchinensis, a perennial vine. the antiulcer effect of an extract from cochinchina momordica seeds (sk-ms10) was evaluated in a rat model of acetic acid-induced gastric ulcers. gastric ulcers were produced by subserosal injection of acetic acid. sk-ms10 (200 mg/kg) or vehicle was administered orally once per day for 14 days after the acetic acid injection. the stomach was removed and the ulcer size measured at day 7 and 14 of the ... | 2010 | 20514308 |
| in vivo evaluation of anthelmintic potential of medicinal plant extracts against dactylogyrus intermedius (monogenea) in goldfish (carassius auratus). | in the present study, an attempt has been made to petroleum ether, chloroform, ethyl acetate, methanol, and water extracts of prunus amygdalus batsch seeds (semen amygdali), cimicifuga foetida l. rhizomes (rhizoma cimicifugae), peucedanum decursivum (miq.) maxim roots (radix peucedani), momordica cochinchinensis spreng. seeds (semen momordicae), and bupleurum chinense dc. roots (radix bupleuri chinensis) for their in vivo anthelmintic activity against monogenean dactylogyrus intermedius in goldf ... | 2010 | 21153837 |
| bioactive proteins and peptides isolated from chinese medicines with pharmaceutical potential. | some protein pharmaceuticals from chinese medicine have been developed to treat cardiovascular diseases, genetic diseases, and cancer. bioactive proteins with various pharmacological properties have been successfully isolated from animals such as hirudo medicinalis (medicinal leech), eisenia fetida (earthworm), and mesobuthus martensii (chinese scorpion), and from herbal medicines derived from species such as cordyceps militaris, ganoderma, momordica cochinchinensis, viscum album, poria cocos, s ... | 2014 | 25067942 |
| enhancement of immune responses to infectious bursal disease vaccine by supplement of an extract made from momordica cochinchinensis (lour.) spreng. seeds. | the immunological effect of an extract from momordica cochinchinensis seed (ecms) on immune responses against infectious bursal disease (ibd) in chickens was evaluated. fifty-two birds were equally divided into 4 groups and immunized with inactivated ibd vaccine alone (controls) or ibd vaccine emulsified with ecms (20, 40, and 80 microg). serum igg antibody levels against ibd and bw were measured on 0, 7, 14, 21, 28, and 35 d after immunization. the elisa results revealed that the chickens that ... | 2010 | 20460658 |
| engineered cystine knot miniproteins as potent inhibitors of human mast cell tryptase beta. | here we report the design, chemical and recombinant synthesis, and functional properties of a series of novel inhibitors of human mast cell tryptase beta, a protease of considerable interest as a therapeutic target for the treatment of allergic asthma and inflammatory disorders. these inhibitors are derived from a linear variant of the cyclic cystine knot miniprotein mcoti-ii, originally isolated from the seeds of momordica cochinchinensis. a synthetic cyclic miniprotein that bears additional po ... | 2010 | 19852971 |
| enhancement of immune responses to newcastle disease vaccine by a supplement of extract of momordica cochinchinensis (lour.) spreng. seeds. | the study evaluated the immunological effect of extract of momordica cochinchinensis (lour.) spreng. seeds (ecms) on the immune response against newcastle disease (nd) in chickens. forty-eight chickens were divided into 4 groups (n = 12). each chicken was immunized with nd vaccine mixed with 0, 20, 40, or 80 microg of ecms on d 35. blood samples were collected on d 0, 7, 14, 21, 28, and 35 postimmunization. humoral and cellular immune responses were evaluated by indirect elisa assay and the 3-(4 ... | 2009 | 19834078 |
| isolation and characterization of peptides from momordica cochinchinensis seeds. | the plant momordica cochinchinensis has traditionally been used in chinese medicine to treat a variety of illnesses. a range of bioactive molecules have been isolated from this plant, including peptides, which are the focus of this study. here we report the isolation and characterization of two novel peptides, mcocc-1 and mcocc-2, containing 33 and 32 amino acids, respectively, which are toxic against three cancer cell lines. the two peptides are highly homologous to one another, but show no seq ... | 2009 | 19711988 |
| in vitro micellarization and intestinal cell uptake of cis isomers of lycopene exceed those of all-trans lycopene. | the ratio of cis and all-trans lycopene (lyc) in human and animal tissues exceeds that in foods. the basis for this difference remains unknown, although differences in their stability, transport, and metabolism have been suggested. here, we systematically compared the digestive stability, efficiency of micellarization, and uptake and intracellular stability of cis and all-trans isomers of lyc and carotenes using the coupled in vitro digestion and caco-2 human intestinal cell model. aril and oil ... | 2008 | 18287353 |
| cochinin b, a novel ribosome-inactivating protein from the seeds of momordica cochinchinensis. | cochinin b, a novel ribosome-inactivating protein (rip) with a molecular weight of 28 kda, was purified from the seeds of momordica cochinchinensis (cucurbitaceae). the isolation procedure entailed ammonium sulfate precipitation, cation-exchange chromatography on sp sepharose column and size-exclusion chromatography on superdex 75 column with a fast protein liquid chromatography (fplc) system. the first twenty n-terminal amino acid residues of cochinin b showed homology to type i rips from other ... | 2007 | 17329832 |
| knots in rings. the circular knotted protein momordica cochinchinensis trypsin inhibitor-ii folds via a stable two-disulfide intermediate. | the aim of this work was to elucidate the oxidative folding mechanism of the macrocyclic cystine knot protein mcoti-ii. we aimed to investigate how the six-cysteine residues distributed on the circular backbone of the reduced unfolded peptide recognize their correct partner and join up to form a complex cystine-knotted topology. to answer this question, we studied the oxidative folding of the naturally occurring peptide using a range of spectroscopic methods. for both oxidative folding and reduc ... | 2006 | 16547012 |
| effects of four different drying methods on the carotenoid composition and antioxidant capacity of dried gac peel. | gac fruit (momordica cochinchinensis spreng.) is a rich source of carotenoids for the manufacture of powder, oil and capsules for food, cosmetic and pharmaceutical uses. currently, only the aril of the gac fruit is processed and the peel, similar to the other components, is discarded, although it contains high level of carotenoids, which could be extracted for commercial use. in the present study, four different drying methods (hot-air, vacuum, heat pump and freeze drying), different temperature ... | 2017 | 27435184 |
| substrate-guided design of selective fxiia inhibitors based on the plant-derived momordica cochinchinensis trypsin inhibitor-ii (mcoti-ii) scaffold. | thrombosis is a leading cause of morbidity and mortality associated with cardiovascular diseases. inhibition of factor xiia (fxiia) provides thrombus protection without bleeding complications. here, we defined the extended substrate specificity of fxiia and its close homologue factor xa and used these data, together with inhibitor-based and structure-guided methods, to engineer selective fxiia inhibitors based on momordica cochinchinensis trypsin inhibitor-ii. | 2016 | 27434175 |
| kalata b8, a novel antiviral circular protein, exhibits conformational flexibility in the cystine knot motif. | the cyclotides are a family of circular proteins with a range of biological activities and potential pharmaceutical and agricultural applications. the biosynthetic mechanism of cyclization is unknown and the discovery of novel sequences may assist in achieving this goal. in the present study, we have isolated a new cyclotide from oldenlandia affinis, kalata b8, which appears to be a hybrid of the two major subfamilies (möbius and bracelet) of currently known cyclotides. we have determined the th ... | 2006 | 16207177 |
| antioxidative effect of a chymotrypsin inhibitor from momordica cochinchinensis (cucurbitaceae) seeds in a primary rat hepatocyte culture. | the antioxidative activity of a chymotrypsin-specific potato type i inhibitor from momordica cochinchinensis (mcoci) (cucurbitaceae) has been investigated using the primary rat hepatocyte system. tert-butyl hydroperoxide (t-bhp) was used to induce oxidative stress. pretreatment of hepatocytes with mcoci for 24 h significantly reversed t-bhp-induced cell damage, and the associated glutathione depletion and lipid peroxidation. the activities of glutathione-s-transferase and superoxide dismutase we ... | 2005 | 15849778 |
| protective effect and mechanism of action of saponins isolated from the seeds of gac (momordica cochinchinensis spreng.) against cisplatin-induced damage in llc-pk1 kidney cells. | this study was performed to investigate the renoprotective effect and mechanism of momordicae semen, gac seeds, against the cisplatin-induced damage in llc-pk1 kidney cells. in order to identify the active components, three major saponins were isolated from extract of the gac seed, gypsogenin 3-o-β-d-galactopyranosyl(1→2)-[α-l-rhamnopyranosyl(1→3)]-β-d-glucuronopyranoside (1), quillaic acid 3-o-β-d-galactopyranosyl(1→2)-[α-l-rhamnopyranosyl(1→3)]-β-d-glucuronopyranoside (2), and momordica saponi ... | 2016 | 26838808 |
| inhibition of tumor growth and angiogenesis by water extract of gac fruit (momordica cochinchinensis spreng). | the antitumor activity of the crude water extract from gac fruit (momordica cochinchinensis) was investigated in vivo and in vitro. a water extract prepared from 0.75 and 0.25 mg dry weight of gac fruit per gram body weight was given daily to balb/c mice (n=15/group). the water extract inhibited the growth of the colon 26-20 adenocarcinoma cell line, transplanted in balb/c mice, reducing wet tumor weight by 23.6%. histological and immunohistochemical results indicated that gac water extract redu ... | 2005 | 15753981 |
| squash trypsin inhibitors from momordica cochinchinensis exhibit an atypical macrocyclic structure. | three trypsin inhibitors (tis), from the seeds of the squash momordica cochinchinensis (mco), have been isolated and purified using gel filtration, ion exchange chromatography, and reverse-phase hplc. their sequences could be determined only after proteolytic cleavages. in the case of mcoti-i and -ii, it was shown that their polypeptide backbones are cyclic, a structure that has never been described in squash tis. they contain 34 amino acid residues with 3 disulfide bridges and measured molecula ... | 2000 | 10801322 |
| isolation and characterization of an abortifacient protein, momorcochin, from root tubers of momordica cochinchinensis (family cucurbitaceae). | a glycoprotein with a molecular weight of 32,000 as estimated by sds-polyacrylamide gel electrophoresis, and characterized by an abundance of asp and glu residues and an absence of cys residues in its amino acid analysis, was isolated from fresh root tubers of momordica cochinchinensis using a procedure that involved acetone precipitation, ammonium sulfate precipitation, ion exchange chromatography on deae sepharose cl-6b and gel filtration on sephadex g-75. the protein was capable of inducing m ... | 1987 | 3667075 |
| purification and properties of a new ribosome-inactivating protein with rna n-glycosidase activity suitable for immunotoxin preparation from the seeds of momordica cochinchinensis. | a ribosome-inactivating protein similar to those already known (stirpe and barbieri (1986) febs lett. 195, 1-8) was purified from the seeds of momordica cochinchinensis. this protein, for which the name of momorcochin-s is proposed, is a glycoprotein, has an mr of approx. 30,000, and an alkaline isoelectric point and can be considered as an iso-form of the previously purified momorcochin from the roots of m. cochinchinensis. momorcochin-s inhibits protein synthesis by a rabbit-reticulocyte lysat ... | 1989 | 2597699 |
| proteins with abortifacient, ribosome inactivating, immunomodulatory, antitumor and anti-aids activities from cucurbitaceae plants. | 1. the biochemical characteristics and biological activities of eight cucurbitaceae plant proteins designated trichosanthin (isolated from tubers of trichosanthes kirilowii), beta-trichosanthin (isolated from tubers of trichosanthes cucumeroides), alpha- and beta-momorcharins (isolated from seeds of momordica charantia), momorchochin (isolated from tubers of momordica cochinchinensis), luffaculin (isolated from seeds of luffa acutangula) and luffin-a and luffin-b (isolated from seeds of luffa cy ... | 1992 | 1397965 |
| biodistribution of the cyclotide mcoti-ii, a cyclic disulfide-rich peptide drug scaffold. | disulfide-rich macrocyclic peptides are promising templates for drug design because of their unique topology and remarkable stability. however, little is known about their pharmacokinetics. in this study, we characterize the biodistribution in mice of momordica cochinchinensis trypsin inhibitor-ii (mcoti-ii), a cyclic three-disulfide-containing peptide that has been used in a number of studies as a drug scaffold. the distribution of mcoti-ii was compared with that of chlorotoxin, which is a four ... | 2016 | 26929247 |
| the geometric approach to explore the bactrocera tau complex (diptera: tephritidae) in thailand. | specimens of the genus bactrocera were collected from several host plants in northern and western thailand. they were morphologically recognized as bactrocera tau and were subdivided into eleven samples according to host plant, geographic origin and time of collection. twelve landmarks of the right wing were described in a total of 264 males and 276 females. an exploratory analysis using kernel density estimates was performed on the multivariate morphometric space. non-parametric classification ... | 2010 | 20817492 |
| phenetic structure of two bactrocera tau cryptic species (diptera: tephritidae) infesting momordica cochinchinensis (cucurbitaceae) in thailand and laos. | morphometric variation with respect to wing venation patterns was explored for 777 specimens of the bactrocera tau complex collected in thailand (nine provinces) and laos (one locality). cryptic species b. tau a and c were identified based on their wing shape similarity to published reference images. in thailand, the b. tau a species was identified in four provinces and the b. tau c species in seven provinces, and both species in one locality of laos. the objective of the study was to explain th ... | 2013 | 23332679 |
| cyclic peptides arising by evolutionary parallelism via asparaginyl-endopeptidase-mediated biosynthesis. | the cyclic miniprotein momordica cochinchinensis trypsin inhibitor ii (mcoti-ii) (34 amino acids) is a potent trypsin inhibitor (ti) and a favored scaffold for drug design. we have cloned the corresponding genes and determined that each precursor protein contains a tandem series of cyclic tis terminating with the more commonly known, and potentially ancestral, acyclic ti. expression of the precursor protein in arabidopsis thaliana showed that production of the cyclic tis, but not the terminal ac ... | 2012 | 22822203 |
| carotenoids concentration of gac (momordica cochinchinensis spreng.) fruit oil using cross-flow filtration technology. | gac (momordica cochinchinensis spreng.) fruit, a traditional fruit in vietnam and other countries of eastern asia, contains an oil rich in carotenoids, especially lycopene and β-carotene. carotenoids in gac fruit oil were concentrated using cross-flow filtration. in total recycle mode, effect of membrane pore size, temperature, and transmembrane pressure (tmp) on permeate flux and on retention coefficients has been exploited. resistance of membrane, polarization concentration, and fouling were a ... | 2014 | 25367308 |
| combinatorial optimization of cystine-knot peptides towards high-affinity inhibitors of human matriptase-1. | cystine-knot miniproteins define a class of bioactive molecules with several thousand natural members. their eponymous motif comprises a rigid structured core formed by six disulfide-connected cysteine residues, which accounts for its exceptional stability towards thermic or proteolytic degradation. since they display a remarkable sequence tolerance within their disulfide-connected loops, these molecules are considered promising frameworks for peptide-based pharmaceuticals. natural open-chain cy ... | 2013 | 24146945 |
| cystine-knot peptides targeting cancer-relevant human cytotoxic t lymphocyte-associated antigen 4 (ctla-4). | cystine-knot peptides sharing a common fold but displaying a notably large diversity within the primary structure of flanking loops have shown great potential as scaffolds for the development of therapeutic and diagnostic agents. in this study, we demonstrated that the cystine-knot peptide mcoti-ii, a trypsin inhibitor from momordica cochinchinensis, can be engineered to bind to cytotoxic t lymphocyte-associated antigen 4 (ctla-4), an inhibitory receptor expressed by t lymphocytes, that has emer ... | 2015 | 25964162 |
| a new family of cystine knot peptides from the seeds of momordica cochinchinensis. | momordica cochinchinensis, a cucurbitaceae plant commonly found in southeast asia, has the unusual property of containing both acyclic and backbone-cyclized trypsin inhibitors with inhibitor cystine knot (ick) motifs. in the current study we have shown that m. cochinchinensis also contains another family of acyclic ick peptides. we recently reported two novel peptides from m. cochinchinensis but have now discovered four additional peptides (mco-3-mco-6) with related sequences. together these pep ... | 2013 | 23127518 |
| anti-gastritis and wound healing effects of momordicae semen extract and its active component. | momordicae semen, momordica cochinchinensis springer (cucurbitaceae), has long been known to effectively relieve boils, rheumatic pain, and hemorrhoids. in this study, we investigated whether momordicae semen extract (mse) has anti-gastritis effects in various rodent models and also explored possible mechanisms for the gastroprotective effects of mse. mse provided remarkable protective effects, comparable to those of rebamipide, in ethanol- and diclofenac-induced acute gastritis. in addition, it ... | 2013 | 22889079 |
| evaluation of direct antiviral activity of the deva-5 herb formulation and extracts of five asian plants against influenza a virus h3n8. | the herb formulation deva-5 is used in traditional medicine to treat acute infectious diseases. deva-5 is composed of five herbs: gentiana decumbens l., momordica cochinchinensis l., hypecoum erectum l., polygonum bistorta l., and terminalia chebula retz. deva-5 and its five components were investigated for in vitro antiviral activity against avian influenza a virus subtype h3n8. | 2014 | 25012588 |
| engineering pro-angiogenic peptides using stable, disulfide-rich cyclic scaffolds. | fragments from the extracellular matrix proteins laminin and osteopontin and a sequence from vegf have potent proangiogenic activity despite their small size (< 10 residues). however, these linear peptides have limited potential as drug candidates for therapeutic angiogenesis because of their poor stability. in the present study, we show that the therapeutic potential of these peptides can be significantly improved by "grafting" them into cyclic peptide scaffolds. momordica cochinchinensis tryps ... | 2011 | 22039263 |
| alkanes in flower surface waxes of momordica cochinchinensis influence attraction to aulacophora foveicollis lucas (coleoptera: chrysomelidae). | extraction, thin-layer chromatography, and gas chromatography-mass spectrophotometry analyses revealed 15 alkanes representing 97.14% of the total alkanes in the surface waxes of momordica cochinchinensis spreng flowers. nonacosane was the prevailing alkane followed by hexatriacontane, nonadecane, heptacosane, and hentriacontane, accounting for 39.08%, 24.24%, 13.52%, 6.32%, and 5.12%, respectively. the alkanes from flower surface waxes followed by a synthetic mixture of alkanes mimicking alkane ... | 2013 | 23949856 |
| high-affinity cyclic peptide matriptase inhibitors. | sunflower trypsin inhibitor-1 (sfti-1) and momordica cochinchinensis trypsin inhibitor-ii (mcoti-ii) are potent protease inhibitors comprising a cyclic backbone. | 2013 | 23548907 |
| comparative analysis of carotenoid content in momordica cochinchinensis (cucurbitaceae) collected from australia, thailand and vietnam. | momordica cochinchinensis (cucurbitaceae) is the richest source of lycopene and β-carotene of all known fruits but the influences of collection sites, variety and environment on carotenoid accumulation is unknown. this study analysed the carotenoid content of 44 m. cochinchinensis aril samples collected from australia, thailand and vietnam using hplc, uv-visible spectrophotometry and compared with the colorimetry method. the highest lycopene content was observed in samples collected from ha noi ... | 2017 | 28928521 |
| carotenoids from gac fruit aril (momordica cochinchinensis [lour.] spreng.) are more bioaccessible than those from carrot root and tomato fruit. | using a simulated digestion procedure in vitro, liberation and bioaccessibility of β-carotene (29.5±1.7% and 22.6±0.9%, respectively) and lycopene (51.3±2.6% and 33.2±3.1%, respectively) from gac fruit aril were found to be significantly higher than from carrot root (β-carotene, 5.2±0.5% and 0.5±0.2%, respectively) and tomato fruit (lycopene, 15.9±2.8% and 1.8±0.5%, respectively). gac fruit aril naturally contained significantly more lipids (11% on fresh weight base) than carrot root and tomato ... | 2017 | 28847429 |
| src/syk-targeted anti-inflammatory actions of triterpenoidal saponins from gac (momordica cochinchinensis) seeds. | momordica cochinchinensis spreng (family cucurbitaceae), also known as gac, or red melon, is an edible southeast asian fruit valued for its nutritional and medicinal properties. specifically, momordicae semen, the seeds of the gac fruit, is used in traditional chinese medicine to treat boils, rheumatic pain, muscle spasm, hemorrhoids, and hemangiomas. in this study, a chemical investigation into a gac seed ethanol (etoh) extract resulted in the identification of three triterpenoidal saponins (1- ... | 2017 | 28367713 |
| dual-targeting anti-angiogenic cyclic peptides as potential drug leads for cancer therapy. | peptide analogues derived from bioactive hormones such as somatostatin or certain growth factors have great potential as angiogenesis inhibitors for cancer applications. in an attempt to combat emerging drug resistance many fda-approved anti-angiogenesis therapies are co-administered with cytotoxic drugs as a combination therapy to target multiple signaling pathways of cancers. however, cancer therapies often encounter limiting factors such as high toxicities and side effects. here, we combined ... | 2016 | 27734947 |
| two new oleanane-type triterpenoids from methanolyzed saponins of momordica cochinchinensis. | two new oleanane-type triterpenoid saponins (1 and 2) were isolated from the methanolyzed total saponins of the seeds of momordica cochinchinensis (lour.) spreng, together with 16 known compounds (3-18). their structures were elucidated on the basis of detailed spectroscopic, including 1d and 2d nmr, mass spectrometric, methanolysis and lc-ms analysis. all the isolates were tested for their cytotoxic activities against five human cancer cell lines (hl-60, smmc-7721, panc-1, a-549, and sw-480) an ... | 2016 | 27534102 |
| plants defense-related cyclic peptides: diversity, structure and applications. | plant growth is prone to several unfavorable factors that may compromise or impair development and survival, including abiotic or biotic stressors. aiming at defending themselves, plants have developed several strategies to survive and adapt to such adversities. cyclotides are a family of plant-derived proteins that exhibit a diverse range of biological activities including antimicrobial and insecticidal activities that actively participate in plant defense processes. three main categories of pe ... | 2016 | 27455973 |
| immunomodulatory activity of a chymotrypsin inhibitor from momordica cochinchinensis seeds. | serine protease inhibitors are widely distributed in the plant kingdom. many of them have been purified and characterized from different species. while the physicochemical properties of these protease inhibitors have been extensively investigated, their biological effects, e.g. immunomodulatory effect, remain relatively unexplored. recently, we isolated a chymotrypsin-specific inhibitor (mcoci) from the seeds of momordica cochinchinensis (lour) spreng (family cucurbitaceae), the traditional chin ... | 2006 | 16733830 |
| effects of maturity on physicochemical properties of gac fruit (momordica cochinchinensis spreng.). | the aril around the seeds of gac fruit is rich in fatty acids and carotenoids (lycopene and β-carotene). understanding how these qualities are affected by fruit maturity at harvest may identify indices for quality assessment. some physical and chemical properties of gac fruit were determined for fruit harvested between 8 and 16 weeks after pollination (wap). fruit respiration rates and ethylene production rates were assessed after harvest and up to 20 days in storage at 20°c. fruit harvested at ... | 2016 | 27004120 |
| computational analysis of the mcoti-ii plant defence knottin reveals a novel intermediate conformation that facilitates trypsin binding. | mcoti-i and ii are plant defence proteins, potent trypsin inhibitors from the bitter gourd momordica cochinchinensis. they are members of the knottin family, which display exceptional stability due to unique topology comprising three interlocked disulfide bridges. knottins show promise as scaffolds for new drug development. a crystal structure of trypsin-bound mcoti-ii suggested that loop 1, which engages the trypsin active site, would show decreased dynamics in the bound state, an inference at ... | 2016 | 26975976 |
| first report on a potato i family chymotrypsin inhibitor from the seeds of a cucurbitaceous plant, momordica cochinchinensis. | a 7514-da chymotrypsin inhibitor was isolated from the seed extract of momordica cochinchinensis (family cucurbitaceae) by chromatography on chymotrypsin-sepharose 4b and subsequently by c18 reversed-phase hplc. this inhibitor, named mcocl, possessed remarkable thermostability and was stable from ph 2 to 12. mcocl also inhibited subtilisin, but had at least 50-fold lower inhibitory activity towards trypsin and elastase. amino acid sequencing of a peptide fragment of mcocl revealed a sequence of ... | 2004 | 15101561 |
| using the mcoti-ii cyclotide scaffold to design a stable cyclic peptide antagonist of set, a protein overexpressed in human cancer. | the set protein is a promising drug target in cancer therapy, because of its ability to inhibit the function of the tumor suppressor gene protein phosphatase 2a (pp2a). cog peptides, derived from apolipoprotein e (apoe), are potent antagonists of set; they induce cytotoxicity in cancer cells upon binding to intracellular set and modulate the nuclear factor kappa b (nf-κb) signaling pathway. however, the therapeutic potential of cog peptides is limited, because of their poor proteolytic stability ... | 2016 | 26685975 |
| cyclic thrombospondin-1 mimetics: grafting of a thrombospondin sequence into circular disulfide-rich frameworks to inhibit endothelial cell migration. | tumour formation is dependent on nutrient and oxygen supply from adjacent blood vessels. angiogenesis inhibitors can play a vital role in controlling blood vessel formation and consequently tumour progression by inhibiting endothelial cell proliferation, sprouting and migration. the primary aim of the present study was to design cyclic thrombospondin-1 (tsp-1) mimetics using disulfide-rich frameworks for anti-angiogenesis therapies and to determine whether these peptides have better potency than ... | 2015 | 26464514 |
| carotenoid pigments in gac fruit (momordica cochinchinensis spreng). | the carotenoids in gac fruit (momordica cochinchinensis spreng) were analysed by high-performance liquid chromatography (hplc), and the concentrations of beta-carotene, lycopene, zeaxanthin and beta-cryptoxanthin were measured. lycopene was found to be predominantly present in the gac seed membrane at a concentration of up to 380 microg/g of seed membrane. the concentration of lycopene in the gac seed membrane was about ten-fold higher than that in known lycopene-rich fruit and vegetables, indic ... | 2002 | 12506992 |
| clinical evaluation of gac extract (momordica cochinchinensis) in an antiwrinkle cream formulation. | the objective of this work was to evaluate the antioxidant and antityrosinase activities of gac (momordica cochinchinensis) extract and to clinically evaluate a gac-containing antiwrinkle cream formulation. gac extract exhibited higher antioxidant activity than vitamin c or e, as measured by 2,2-diphenyl-1-picrylhydrazyl (dpph, 41.25 ± 0.34 mg teac/ml extract), 2, 2'-azinobis 3-ethylbenzothialine-6-sulfonic acid (abts, 47.70 ± 0.18 mg teac/ml extract), and ferric reducing antioxidant power (frap ... | 2015 | 26454905 |
| momordica cochinchinensis spreng. seed extract suppresses breast cancer growth by inducing cell cycle arrest and apoptosis. | the herb momordica cochinchinensis has been used for a variety of purposes, and been shown to have anti‑cancer properties. the present study assessed the potency and the underlying mechanisms of action of the ethyl acetate extract of seeds of momordica cochinchinensis (esmc2) on breast cancer cells. therefore, the effects of esmc2 on the cell viability, cell cycle and apoptosis of mda‑mb‑231 cells were investigated. the results showed that esmc2 exerted a marked growth inhibitory effect on the c ... | 2015 | 26252798 |
| multiple trypsin inhibitors from momordica cochinchinensis seeds, the chinese drug mubiezhi. | five trypsin inhibitors, with n-terminal sequences demonstrating homology to each other and exhibiting a molecular weight of 5100, 4800, 4400, 4100, and 3900, respectively, were isolated from momordica cochinchinensis seeds with a protocol involving acid extraction, ion exchange chromatography on sp-sepharose chromatography, and rp-hplc on a c18 column. specific inhibitory activity against trypsin was demonstrated by the trypsin isoinhibitors with ki values ranging from 5.3 x 10(-8) to 1.8 x 10( ... | 2004 | 15062996 |
| neurotrophic effects of mu bie zi (momordica cochinchinensis) seed elucidated by high-throughput screening of natural products for ngf mimetic effects in pc-12 cells. | post-mitotic central nervous system (cns) neurons have limited capacity for regeneration, creating a challenge in the development of effective therapeutics for spinal cord injury or neurodegenerative diseases. furthermore, therapeutic use of human neurotrophic agents such as nerve growth factor (ngf) are limited due to hampered transport across the blood brain barrier (bbb) and a large number of peripheral side effects (e.g. neuro-inflammatory pain/tissue degeneration etc.). therefore, there is ... | 2015 | 25862192 |
| cochinchina momordica seed suppresses proliferation and metastasis in human lung cancer cells by regulating multiple molecular targets. | cochinchina momordica seed, which is the dried ripe seed of momordica cochinchinensis (lour.) spreng, has been used as a mainly anticancer ingredient for many years in china. this study aims at investigating the roles of an ethanol-soluble extract of cochinchina momordica seed (ecms) in suppressing the proliferation and metastasis of human lung cancer cells, and further elucidating underlying molecular mechanisms. our researches suggest that ecms dose-dependently decreased the survival rates of ... | 2015 | 25649746 |
| studies on kochiae fructus. v. antipruritic effects of oleanolic acid glycosides and the structure-requirement. | we examined the antipruritic effects of various oleanolic acid glycosides from natural medicines such as kochiae fructus (the fruit of kochia scoparia schrad.) and momordicae radix (the roots of momordica cochinchinensis spreng.) using a compound 48/80-induced pruritic model in mice. oleanolic acid 3-o-monodesmosides showed an antipruritic effect, while oleanolic acid 3,28-o-bisdesmosides and their common sapogenol oleanolic acid lacked the activity. this evidence indicated that the 3-o-glycosid ... | 1998 | 9853421 |
| oxidative metabolites of lycopene and γ-carotene in gac (momordica cochinchinensis). | three new oxidative metabolites of lycopenes, (erythro)-lycopene-5,6-diol, (threo)-lycopene-5,6-diol, and 1,16-dehydro-2,6-cyclolycopene-5-ol b, and four new oxidative metabolites of γ-carotenes, 2',6'-cyclo-γ-carotene-1',5'-diol a, 2',6'-cyclo-γ-carotene-1',5'-diol b, (erythro)-γ-carotene-5,6-diol, and (threo)-γ-carotene-5,6-diol, were isolated as minor components from the aril of gac, momordica cochinchinensis. these structures were determined on the basis of spectroscopic data, and some of th ... | 2015 | 25633727 |
| fragmentation follows structure: top-down mass spectrometry elucidates the topology of engineered cystine-knot miniproteins. | over the last decades the field of pharmaceutically relevant peptides has enormously expanded. among them, several peptide families exist that contain three or more disulfide bonds. in this context, elucidation of the disulfide patterns is extremely important as these motifs are often prerequisites for folding, stability, and activity. an example of this structure-determining pattern is a cystine knot which comprises three constrained disulfide bonds and represents a core element in a vast numbe ... | 2014 | 25303319 |
| a steryl glycoside fraction with hemolytic activity from tubers of momordica cochinchinensis. | a hemolytic fraction has been obtained from fresh tubers of momordica cochinchinensis. the fraction was strongly adsorbed on deae-sepharose cl6b. it did not stain with coomassie brilliant blue in sds-polyacrylamide gel electrophoresis and it gave no immunoprecipitin arcs in immunoelectrophoresis. the hemolytic activity of the fraction was resistant to heat and proteolytic enzymes. the behavior of the fraction in thin-layer chromatography and its positive reaction in liebermann-burchard test indi ... | 1986 | 3821135 |
| structural parameters modulating the cellular uptake of disulfide-rich cyclic cell-penetrating peptides: mcoti-ii and sfti-1. | peptides are emerging as a new class of therapeutics due to their high potency and specificity for a range of targets, including the inhibition of protein-protein interactions. disulfide-rich cyclic peptides, in particular, have attracted much attention in drug design due to their ultra-stable structure. moreover, some of them have been shown to internalize into cells, which makes them potential scaffolds to deliver pharmaceutically bioactive sequences to intracellular targets. here we examined ... | 2014 | 24985034 |
| potent and specific inhibition of the biological activity of the type-ii transmembrane serine protease matriptase by the cyclic microprotein mcoti-ii. | matriptase is a type-ii transmembrane serine protease involved in epithelial homeostasis in both health and disease, and is implicated in the development and progression of a variety of cancers. matriptase mediates its biological effects both via as yet undefined substrates and pathways, and also by proteolytic cleavage of a variety of well-defined protein substrates, several of which it shares with the closely-related protease hepsin. development of targeted therapeutic strategies will require ... | 2014 | 24696092 |
| isomerization and increase in the antioxidant properties of lycopene from momordica cochinchinensis (gac) by moderate heat treatment with uv-vis spectra as a marker. | momordica cochinchinensis (gac) is a plant rich in lycopene. this pigment tends to solubilize in oil and get damaged during extraction. the impact of heating on cis-isomerization of oil-free lycopene in hexane was studied at 50 and 80°c during 240min with uv-vis spectrometry, dad-hplc and teac test. the initial all-trans-form isomerized to the 13-cis isomer more rapidly at 80°c. after this treatment, 16% of the lycopene compounds were in the 9-cis-form. this isomer triggered an increase in the a ... | 2014 | 24629938 |
| a comparative study of extraction methods reveals preferred solvents for cystine knot peptide isolation from momordica cochinchinensis seeds. | mcoti-i and mcoti-ii (short for momordica cochinchinensis trypsin inhibitor-i and -ii, respectively) are attractive candidates for developing novel intracellular-targeting drugs because both are exceptionally stable and can internalize into cells. these seed-derived cystine knot peptides are examples of how natural product discovery efforts can lead to biomedical applications. however, discovery efforts are sometimes hampered by the limited availability of seed materials, highlighting the need f ... | 2014 | 24613804 |
| momordica cochinchinensis seed extracts suppress migration and invasion of human breast cancer zr-75-30 cells via down-regulating mmp-2 and mmp-9. | metastases and invasion are the main reasons for oncotherapy failure. momordica cochinchinensis (mu bie zi in chinese) had been used for a variety of purposes, and shown anti-cancer action. in this article, we focused on effects on regulation of breast cancer cell zr-75-30 metastases and invasion by extracts of momordica cochinchinensis seeds (esmcs). | 2014 | 24606426 |
| cochinchina momordica seed extract induces apoptosis and cell cycle arrest in human gastric cancer cells via parp and p53 signal pathways. | cochinchina momordica seed is the dried ripe seed of momordica cochinchinensis (lour.) spreng, which is a kind of fruit and consumed for dietary as well as medicinal uses. in this study, using the human sgc7901 and mkn-28 gastric cancer cell lines, we explored the anticancer activity of the extract from cochinchina momordica seed (ecms). ecms inhibited significantly the survival rates of sgc7901 and mkn-28 cells in concentration- and time-dependent manners by mtt assay. the typical apoptotic mor ... | 2012 | 23020228 |
| anti-inflammatory properties of a triterpenoidal glycoside from momordica cochinchinensis in lps-stimulated macrophages. | two triterpenoidal saponins were isolated from the seeds of momordica cochinchinensis sprenger (cucurbitaceae). identification of chemical structures has been performed by (1)h- and (13)c-nmr spectroscopy and gas chromatography (gc). one of the saponins is a new gypsogenin glycoside, named as gypsogenin 3-o-β-d-galactopyranosyl(1→2)-[α-l-rhamnopyranosyl(1→3)]-β-d-glucuronopyranoside (compound 1), which is reported for the first time from natural resources. the other saponin is a quillaic acid gl ... | 2013 | 22916793 |
| de novo transcriptome sequencing of momordica cochinchinensis to identify genes involved in the carotenoid biosynthesis. | the ripe fruit of momordica cochinchinensis spreng, known as gac, is featured by very high carotenoid content. although this plant might be a good resource for carotenoid metabolic engineering, so far, the genes involved in the carotenoid metabolic pathways in gac were unidentified due to lack of genomic information in the public database. in order to expedite the process of gene discovery, we have undertaken illumina deep sequencing of mrna prepared from aril of gac fruit. from 51,446,670 high- ... | 2012 | 22580955 |
| identification and characterization of a new family of cell-penetrating peptides: cyclic cell-penetrating peptides. | cell-penetrating peptides can translocate across the plasma membrane of living cells and thus are potentially useful agents in drug delivery applications. disulfide-rich cyclic peptides also have promise in drug design because of their exceptional stability, but to date only one cyclic peptide has been reported to penetrate cells, the momordica cochinchinensis trypsin inhibitor ii (mcoti-ii). mcoti-ii belongs to the cyclotide family of plant-derived cyclic peptides that are characterized by a cy ... | 2011 | 21873420 |
| phytochemicals and antioxidant activity of different fruit fractions (peel, pulp, aril and seed) of thai gac (momordica cochinchinensis spreng). | three fractions (peel, pulp and aril) of gac fruit (momordica cochinchinensis spreng) were investigated for their phytochemicals (lycopene, beta-carotene, lutein and phenolic compounds) and their antioxidant activity. the results showed that the aril had the highest contents for both lycopene and beta-carotene, whilst peel (yellow) contained the highest amount of lutein. two major phenolic acid groups: hydroxybenzoic acids and hydroxycinnamic were identified and quantified. gallic acid and p-hyd ... | 2011 | 25214106 |
| [chemical constituents of unsaponifiable matter from seed oil of momordica cochinchinensis]. | to study the chemical constituents of unsaponifiable matter from the seed oil of momordica cochinchinensis. | 2006 | 17087086 |
| trypsin inhibition by macrocyclic and open-chain variants of the squash inhibitor mcoti-ii. | mcoti-i and mcoti-ii from the seeds of momordica cochinchinensis are inhibitors of trypsin-like proteases and the only known members of the large family of squash inhibitors that are cyclic and contain an additional loop connecting the amino- and the carboxy-terminus. to investigate the contribution of macrocycle formation to biological activity, we synthesized a set of open-chain variants of mcoti-ii that lack the cyclization loop and contain various natural and non-natural amino acid substitut ... | 2005 | 16336125 |
| squash inhibitors: from structural motifs to macrocyclic knottins. | in this article, we will first introduce the squash inhibitor, a well established family of highly potent canonical serine proteinase inhibitors isolated from cucurbitaceae. the squash inhibitors were among the first discovered proteins with the typical knottin fold shared by numerous peptides extracted from plants, animals and fungi. knottins contain three knotted disulfide bridges, two of them arranged as a cystine-stabilized beta-sheet motif. in contrast to cyclotides for which no natural lin ... | 2004 | 15551519 |
| fatty acid and carotenoid composition of gac (momordica cochinchinensis spreng) fruit. | in this study, we analyzed fatty acid and carotenoid composition of fruit tissues, including seed (which are surrounded by a bright red, oily aril) of momordica cochinchinensis spreng, known as gac in vietnam. carotenoid content was analyzed by reversed-phase hplc, using a c(30) column and a method separating cis- and trans-isomers of the major carotenoids in this fruit. mean values obtained in aril tissues were 1342 microg trans-, 204 microg cis-, and 2227 microg total lycopene; 597 microg tran ... | 2004 | 14733508 |
| a method of preserving and testing the acceptability of gac fruit oil, a good source of beta-carotene and essential fatty acids. | gac fruit (momordica cochinchinensis spreng) is indigenous to vietnam and other countries in southeast asia. its seed pulp contains high concentrations of carotenoids, especially the provitamin a, beta-carotene. in northern vietnam, gac fruits are seasonal and are mainly used in making a rice dish called xoi gac. the purpose of this study was to develop a method to collect and preserve gac fruit oil, to evaluate the nutritional composition of the oil, and to assess the acceptability of the gac o ... | 2003 | 12891827 |
| momordica cochinchinensis aril extract induced apoptosis in human mcf-7 breast cancer cells. | momordica cochinchinensis spreng (mc) has been used in traditional medicine due to its high carotenoid content. the objective of this study was to investigate mechanisms underlying apoptotic effects of mc on human mcf-7 breast cancer cells. a lycopene-enriched aril extract of mc (ae) showed cytotoxicity and antiestrogenicity to mcf-7 cells. on dapi staining, ae induced cell shrinkage and chromatin condensation were evident. with flow cytometric analysis, ae increased the percentage of cells in a ... | 2015 | 26225702 |
| effect of heat-processing on the antioxidant and prooxidant activities of β-carotene from natural and synthetic origins on red blood cells. | extraction of bioactives is a cause of structural changes in these molecules. in this work, the bioactivity of commercial natural β-carotenes, one softly extracted without heat-assistance from momordica cochinchinensis (bcg), one conventionally extracted from another natural source (bcc), and a synthetic one (bcs), was assessed during an additional heat-treatment mimicking formulation. their antioxidant activities were evaluated after heat-treatment at different concentrations through hemolysis ... | 2016 | 26213087 |
| circular proteins in plants: solution structure of a novel macrocyclic trypsin inhibitor from momordica cochinchinensis. | much interest has been generated by recent reports on the discovery of circular (i.e. head-to-tail cyclized) proteins in plants. here we report the three-dimensional structure of one of the newest such circular proteins, mcoti-ii, a novel trypsin inhibitor from momordica cochinchinensis, a member of the cucurbitaceae plant family. the structure consists of a small beta-sheet, several turns, and a cystine knot arrangement of the three disulfide bonds. interestingly, the molecular topology is simi ... | 2001 | 11292835 |
| isolation of a trypsin inhibitor with deletion of n-terminal pentapeptide from the seeds of momordica cochinchinensis, the chinese drug mubiezhi. | a trypsin inhibitor, mccti-1, with a molecular weight of 3479 da as determined by mass spectrometry, was isolated from momordica cochinchinensis seeds with a procedure involving extraction with 5% acetic acid, ammonium sulfate precipitation, ion exchange chromatography on cm-sepharose and reverse-phase high performance liquid chromatography. the sequence of its first 13 n-terminal amino acid residues was ilkkcrrdsdcpg which was about 85% identical with the sequence of trypsin inhibitor mcti-1 fr ... | 1999 | 10404643 |
| isolation of a ribosome-inactivating and abortifacient protein from seeds of luffa acutangula. | a glycoprotein with a molecular weight of 28,000 as estimated by sds-polyacrylamide gel electrophoresis was isolated from seeds of luffa acutangula using a procedure that involved acetone precipitation, ion exchange chromatography on cm sepharose cl-6b and gel filtration on sephadex g-50. in immunodiffusion studies it was found to be immunologically distinct from abortifacient proteins isolated from other members of the cucurbitaceae family including momordica charantia, momordica cochinchinensi ... | 1991 | 1938101 |
| chondrillasterol from the tubers of momordica cochinchinensis. | | 1987 | 17269107 |
| plasma beta-carotene and retinol concentrations of children increase after a 30-d supplementation with the fruit momordica cochinchinensis (gac). | in rural vietnam, vitamin a deficiency is a concern. among the indigenous fruit and vegetables, momordica cochinchinensis (gac) fruit has been identified as having the highest beta-carotene concentration. locally, it is mixed with rice in a preparation called xoi gac. | 2002 | 11976161 |
| a new multiflorane triterpenoid ester from momordica cochinchinensis spreng. | a new triterpenoid ester 3,29-di-o-(p-methoxy)benzoylmultiflora-8-ene-3 alpha,29-diol-7-one from the seeds of momordica cochinchinensis spreng has been isolated. its structure was elucidated by spectroscopic evidence and confirmed by x-ray crystallography. | 2001 | 11561447 |
| solution structure of the squash trypsin inhibitor mcoti-ii. a new family for cyclic knottins. | the "knottin" fold is a stable cysteine-rich scaffold, in which one disulfide crosses the macrocycle made by two other disulfides and the connecting backbone segments. this scaffold is found in several protein families with no evolutionary relationships. in the past few years, several homologous peptides from the rubiaceae and violaceae families were shown to define a new structural family based on macrocyclic knottin fold. we recently isolated from momordica cochinchinensis seeds the first know ... | 2001 | 11434766 |