| [chemical and biological characterization of meal and protein isolates from pumpkin seed (cucurbita moschata)]. | the present study was carried out in order to check through chemical and biological analyses the nutritional characteristics of pumpkin seed, its delipidized meal and its proteic concentrate, considering its availability, nutritional potential, facility for production in poor soils and the need for new food resources. another objective was to complement the amino acid pattern of pumpkin with others protein sources for human consumption. the results obtained indicate that: raw pumpkin seed meal h ... | 1992 | 1342182 |
| substrate specificity modification of the stromal glycerol-3-phosphate acyltransferase. | the stromal glycerol-3-phosphate acyltransferases (gpats; ec 2.3.1.15) from spinach (spinacia oleracea) and squash (cucurbita moschata) were expressed in escherichia coli and their activities with palmitoyl-coa and oleoyl-coa compared. the gpat from squash, a chilling-sensitive plant, was found to have the greatest difference in activities between the two substrates, using palmitoyl-coa over three times faster than oleoyl-coa. in contrast, the enzyme from spinach, a chilling-tolerant plant, pref ... | 1997 | 9016814 |
| induction of cutinolytic esterase activity during saprophytic growth of cucurbit pathogens, fusarium solani f. sp. cucurbitae races one and two (nectria haematococca mpi and mpv, respectively). | cutins from fruit of cucurbita maxima and cucurbita moschata cultivars, apple and a c(16) alcohol (hexadecanol) were used to induce cutinolytic esterase activity during saprophytic growth of strains of the two cucurbit pathogens, fusarium solani f. sp. cucurbitae, race 1 (nectria haematococca mating population (mpi) and f. solani f. sp. cucurbitae, race 2 (mpv). four strains of mpv and 11 strains of mpi were were included in the study. although we were primarily interested in the two cucurbit pa ... | 2001 | 11164297 |
| mutagenesis of squash (cucurbita moschata) glycerol-3-phosphate acyltransferase (gpat) to produce an enzyme with altered substrate selectivity. | in an attempt to rationalize the relationship between structure and substrate selectivity of glycerol-3-phosphate acyltransferase (gpat, 1at, ec 2.3.1.15) we have cloned a number of cdnas into the pet overexpression system using a pcr-based approach. following assay of the recombinant enzyme we noted that the substrate selectivity of the squash (cucurbita moschata) enzyme had altered dramatically. this form of gpat has now been crystallized and its full three-dimensional structure elucidated. si ... | 2000 | 11171168 |
| purification and characterization of moschins, arginine-glutamate-rich proteins with translation-inhibiting activity from brown pumpkin (cucurbita moschata) seeds. | from fresh brown pumpkin seeds, two proteins with a molecular mass of 12kda and an n-terminal sequence rich in arginine and glutamate residues were obtained. the protein designated alpha-moschin closely resembled the fruitfly programmed-cell death gene product and the protein designated beta-moschin demonstrated striking similarity to prepro 2s albumin in n-terminal sequence. alpha- and beta-moschins inhibited translation in the rabbit reticulocyte lysate system with an ic(50) of 17 microm and 3 ... | 2002 | 12356464 |
| purification and characterization of moschatin, a novel type i ribosome-inactivating protein from the mature seeds of pumpkin (cucurbita moschata), and preparation of its immunotoxin against human melanoma cells. | a novel ribosome-inactivating protein designated moschatin from the mature seeds of pumpkin (cucurbita moschata) has been successively purified to homogeneity, using ammonium sulfate precipitation, cm-cellulose 52 column chromatography, blue sepharose cl-6b affinity column chromatography and fplc size-exclusion column chromatography. moschatin is a type 1 rip with a pi of 9.4 and molecular weight of approximately 29 kd. it is a rrna n-glycosidase and potently blocked the protein synthesis in the ... | 2003 | 14672560 |
| characterization and activity enhancement of the phloem-specific pumpkin pp2 gene promoter. | the promoter of the pumpkin (cucurbita moschata) pp2 gene (designated np) was isolated from the restriction enzyme-digested genomic dna pool by genome walking and its activity and phloem specificity were examined in transgenic tobacco plants by using gus as a reporter. deletion analysis of the promoter revealed that the 473-bp fragment (-465 to + 8 relative to the transcription start site; designated as npii) exhibited similar activity as the full-length np promoter and retained its phloem speci ... | 2004 | 15672837 |
| ribosome-inactivating proteins in edible plants and purification and characterization of a new ribosome-inactivating protein from cucurbita moschata. | the basic protein fraction of tissue extracts from 40 edible plants inhibited cell-free protein synthesis and released adenine from herring sperm dna, thus having adenine glycosylase activity. this suggested the presence of ribosome-inactivating proteins (rips) in the plant extracts. this indication was further strengthened by the presence of the two activities after a partial chromatographic purification of three extracts, including that from lycopersicon esculentum (tomato), which had very low ... | 2006 | 16564632 |
| globulin-specific proteolytic activity in germinating pumpkin seeds as detected by a fluorescence assay method. | the proteolytic activities of alpha-chymotrypsin, trypsin, pepsin, bromelain, and an extract from germinating pumpkin seeds (cucurbita moschata) were determined by their ability to effect the release of 1-anilino-8-naphthalenesulfonate bound to internal hydrophobic sites in intact protein substrates. casein, glyceraldehyde-3-p dehydrogenase, urease, catalase, pumpkin seed globulin, and bovine serum albumin enhanced the fluorescence of 1-anilino-8-naphthalenesulfonate sufficiently to be used as p ... | 1974 | 16659002 |
| characterization of the major integral protein of vacuolar membrane. | the vacuolar membrane of radish (raphanus sativus) taproot contained a large quantity of a protein of 23 kilodaltons that accounted for more than 25% of the total membrane proteins. the protein, tentatively named vm 23, was purified and characterized. vm 23 tends to aggregate at high temperature even in the presence of 1% sodium dodecyl sulfate. the apparent molecular size of vm 23 was estimated to be about 400 kilodaltons by polyacrylamide gel electrophoresis in the presence of 0.1% triton x-10 ... | 1992 | 16668783 |
| food processing methods influence the glycaemic indices of some commonly eaten west indian carbohydrate-rich foods. | glycaemic index (gi) values for fourteen commonly eaten carbohydrate-rich foods processed by various methods were determined using ten healthy subjects. the foods studied were round leaf yellow yam (dioscorea cayenensis), negro and lucea yams (dioscorea rotundata), white and sweet yams (dioscorea alata), sweet potato (solanum tuberosum), irish potato (ipomoea batatas), coco yam (xanthosoma spp.), dasheen (colocasia esculenta), pumpkin (cucurbita moschata), breadfruit (artocarpus altilis), green ... | 2006 | 16925852 |
| [potential allelopathic effects of piper nigrum, mangifera indica and clausena lansium]. | with piper nigrum, mangifera indica and clausena lansium as the donators, this paper studied their potential allelopathic effects on the germination and growth of zea mays, glycine max, cucurbita moschata, arachis hypogaea, raphanus sativus, echinochloa crusgalli, digitaria sanguinalis and stylosanthes guianensis. the results showed that the aqueous extracts of these donators could inhibit the germination and growth of z. mays, g. max, c. moschata, e. crus-galli and d. sanguinalis at high concen ... | 2006 | 17147171 |
| chemical composition and antioxidant activity of an acidic polysaccharide extracted from cucurbita moschata duchesne ex poiret. | a simple and sensitive high-performance capillary electrophoresis (hpce) method was designed for quantitative analysis of the component monosaccharides of an acidic polysaccharide extracted from pumpkin. in this method, the polysaccharide was hydrolyzed into component monosaccharides with 2.0 m trifluoroacetic acid at 100 degrees c for 6 h and then labeled with 1-phenyl-3-methyl-5-pyrazolone, and subsequently the labeled monosaccharide derivatives were separated by hpce. as a result, glucose (21 ... | 2007 | 17511465 |
| flowering locus t protein may act as the long-distance florigenic signal in the cucurbits. | cucurbita moschata, a cucurbit species responsive to inductive short-day (sd) photoperiods, and zucchini yellow mosaic virus (zymv) were used to test whether long-distance movement of flowering locus t (ft) mrna or ft is required for floral induction. ectopic expression of ft by zymv was highly effective in mediating floral induction of long-day (ld)-treated plants. moreover, the infection zone of zymv was far removed from floral meristems, suggesting that ft transcripts do not function as the f ... | 2007 | 17540715 |
| a water-soluble extract from cucurbita moschata shows anti-obesity effects by controlling lipid metabolism in a high fat diet-induced obesity mouse model. | during the screening of a variety of plant sources for their anti-obesity activity, it was found that a water-soluble extract, named pg105, prepared from stem parts of cucurbita moschata, contains potent anti-obesity activities in a high fat diet-induced obesity mouse model. in this animal model, increases in body weight and fat storage were suppressed by 8-week oral administration of pg105 at 500 mg/kg, while the overall amount of food intake was not affected. furthermore, pg105 protected the d ... | 2007 | 17548058 |
| preceramic adoption of peanut, squash, and cotton in northern peru. | the early development of agriculture in the new world has been assumed to involve early farming in settlements in the andes, but the record has been sparse. peanut (arachis sp.), squash (cucurbita moschata), and cotton (gossypium barbadense) macrofossils were excavated from archaeological sites on the western slopes of the northern peruvian andes. direct radiocarbon dating indicated that these plants grew between 9240 and 5500 (14)c years before the present. these and other plants were recovered ... | 2007 | 17600214 |
| content and in-vitro accessibility of pro-vitamin a carotenoids from sri lankan cooked non-leafy vegetables and their estimated contribution to vitamin a requirement. | vitamin a deficiency is a public health problem in sri lanka, which affects especially pre-school children. carrots (daucus carota), pumpkins (cucurbita maxima), squashes (cucurbita moschata) and sweet potatoes (ipomoea batatas) of orange, yellow-fleshed varieties are good sources of provitamin a carotenoids, but have not been studied in sri lanka in terms of the food as eaten. the content of carotenoids in each preparation method and the in-vitro accessibility of beta-carotene and alpha-caroten ... | 2007 | 17852483 |
| atomic resolution structure of cucurmosin, a novel type 1 ribosome-inactivating protein from the sarcocarp of cucurbita moschata. | a novel type 1 ribosome-inactivating protein (rip) designated cucurmosin was isolated from the sarcocarp of cucurbita moschata (pumpkin). besides rrna n-glycosidase activity, cucurmosin exhibits strong cytotoxicities to three cancer cell lines of both human and murine origins, but low toxicity to normal cells. plant genomic dna extracted from the tender leaves was amplified by pcr between primers based on the n-terminal sequence and x-ray sequence of the c-terminal. the complete mature protein s ... | 2008 | 18652900 |
| increase in zucchini yellow mosaic virus symptom severity in tolerant zucchini cultivars is related to a point mutation in p3 protein and is associated with a loss of relative fitness on susceptible plants. | abstract zucchini yellow mosaic virus (zymv, potyvirus) is a very damaging cucurbit virus worldwide. interspecific crosses with resistant cucurbita moschata have led to the release of "resistant" zucchini squash (c. pepo) f(1) hybrids. however, although the resistance is almost complete in c. moschata, the commercial c. pepo hybrids are only tolerant. zymv evolution toward increased aggressiveness on tolerant hybrids was observed in the field and was obtained experimentally. sequence comparisons ... | 2003 | 18943611 |
| starch grains on human teeth reveal early broad crop diet in northern peru. | previous research indicates that the nanchoc valley in northern peru was an important locus of early and middle holocene human settlement, and that between 9200 and 5500 (14)c yr b.p. the valley inhabitants adopted major crop plants such as squash (cucurbita moschata), peanuts (arachis sp.), and cotton (gossypium barbadense). we report here an examination of starch grains preserved in the calculus of human teeth from these sites that provides direct evidence for the early consumption of cultivat ... | 2008 | 19066222 |
| in vitro effects of cucurbita moschata seed extracts on haemonchus contortus. | this study was carried out to evaluate the in vitro effect of cucurbita moschata seed against the parasitic nematode of small ruminants haemonchus contortus. three extracts (aqueous, methanolic and dichloromethane) of c. moschata seed were tested in vitro on four developmental stages of h. contortus using egg hatch assay (eha), larval development assay (lda), l3 migration inhibition (lmi) assay and adult worm motility (awm) assay. the highly significant (p<0.001) ability to stop larval developme ... | 2009 | 19135803 |
| tritrophic effects on survival and development in a coccidophagous coccinellid. | chilocorus nigritus is currently considered one of the most successful biological control agents of armoured scale insects. however, establishment of this beetle in crop pest situations has not always been successful and there are still gaps in our knowledge of its ecology and behaviour. the research involved an examination of tritrophic effects on the survival and development of this common diaspid predator. the effect of a forced change in host plant on the developmental time of the juvenile s ... | 2008 | 19226777 |
| a molecular model for diacylglycerol acyltransferase from mortierella ramanniana var. angulispora. | acyl coa diacylglycerol acyltransferase (dgat, ec 2.3.120) is recognized as a key player of cellular diacylglycerol metabolism. it catalyzes the terminal, yet the committed step in triacylglycerol synthesis using diacylglycerol and fatty acyl coa as substrates. the protein sequence of diacylglycerol acyltransferse (dgat) type 2b in moretierella ramanniana var. angulispora (protein_id = aak84180.1) was retrieved from genbank. however, a structure is not yet available for this sequence. the 3d str ... | 2009 | 19759814 |
| potential mechanisms of hypocholesterolaemic effect of thai spices/dietary extracts. | several thai spices/dietary ingredients were previously shown to have hypocholesterolaemic effects. these studies were mostly conducted in animal models in which the mechanisms of action were not yet well-established. therefore, this study aimed to investigate the potential mechanism of hypocholesterolaemic action of 12 selected plants, namely hibiscus sabdariffa l., moringa oleifera lam., cucurbita moschata duchesne, ananas comosus (l.) merr., zingiber officinale, morus alba l., camellia sinens ... | 2011 | 20623425 |
| glucose-lowering activity of novel tetrasaccharide glyceroglycolipids from the fruits of cucurbita moschata. | two new tetrasaccharide glyceroglycolipids were obtained from pumpkin. the structures of the two compounds were determined using chemical methods and spectroscopic analysis. both compounds demonstrated significant glucose-lowering effects in streptozotocin- and high-fat-diet-induced diabetic mice. | 2010 | 21215628 |
| agrobacterium-mediated transformation of kabocha squash (cucurbita moschata duch) induced by wounding with aluminum borate whiskers. | an efficient genetic transformation method for kabocha squash (cucurbita moschata duch cv. heiankogiku) was established by wounding cotyledonary node explants with aluminum borate whiskers prior to inoculation with agrobacterium. adventitious shoots were induced from only the proximal regions of the cotyledonary nodes and were most efficiently induced on murashige-skoog agar medium with 1 mg/l benzyladenine. vortexing with 1% (w/v) aluminum borate whiskers significantly increased agrobacterium i ... | 2011 | 21400224 |
| genes for resistance to zucchini yellow mosaic in tropical pumpkin. | four cultigens of cucurbita moschata resistant to zucchini yellow mosaic virus were crossed with the susceptible 'waltham butternut' and with each other in order to clarify the mode of inheritance of resistance and relationships among the genes involved. five loci were segregating, with genes for resistance zym-0 and zym-4 carried by 'nigerian local' and one of them also carried by 'nicklow's delight,' zym-1 carried by 'menina,' and zym-6 carried by 'soler.' a recessive gene carried by 'waltham ... | 2011 | 21493595 |
| lectins but not antifungal proteins exhibit anti-nematode activity. | a variety of lectins and antifungal proteins were tested for toxicity against the plant parasitic nematodes ditylenchus dipsaci and heterodera glycines. it was found that lectins from the mushrooms xylaria hypoxylon, agrocybe cylindracea and tricholoma mongolicum (tml-1) were the most potent against d. dipsaci, with ec(50) being 4.7, 9, and 20mg/ml, respectively. lectins from pseudostellaria heterophylla, samta tomato, and the mushrooms t. mongolicum (tml-2), ganoderma lucidum, and boletus eduli ... | 2009 | 21784014 |
| cucurmosin induces apoptosis of bxpc-3 human pancreatic cancer cells via inactivation of the egfr signaling pathway. | pancreatic cancer remains the fourth most common cause of cancer-related death in the united states. potent therapeutic strategies are urgently needed for pancreatic cancer. cucurmosin is a novel type 1 ribosome-inactivating protein (rip) isolated from the sarcocarp of cucurbita moschata (pumpkin). due to its cytotoxicity, cucurmosin can inhibit tumor cell proliferation through induction of apoptosis on tumor cells, but the specific mec ... | 2011 | 22139427 |
| dehydrodiconiferyl alcohol isolated from cucurbita moschata shows anti-adipogenic and anti-lipogenic effects in 3t3-l1 cells and primary mouse embryonic fibroblasts. | a water-soluble extract from the stems of cucurbita moschata, code named pg105, was previously found to contain strong anti-obesity activities in a high fat diet-induced obesity mouse model. one of its biological characteristics is that it inhibits 3t3-l1 adipocytes differentiation. to isolate the biologically active compound(s), conventional solvent fractionation was performed, and the various fractions were tested for anti-adipogenic activity using oil red o staining method. a single spot on t ... | 2012 | 22262865 |
| tomato leaf curl new delhi virus is associated with pumpkin leaf curl: a new disease in northern india. | during 2006, pumpkin leaf curl-a new disease was observed in the experimental field at indian agricultural research institute. the disease was characterized by upward leaf curl with chlorotic patches and stunting of plant. polymerase chain reaction (pcr) with coat protein specific primers to tomato leaf curl new delhi virus (tolcndv) indicated association of a begomovirus with the disease. the sequence comparison and phylogenetic analysis of the complete dna genome further revealed the identity ... | 2011 | 23730002 |
| molecular characterization of tomato leaf curl palampur virus and pepper leaf curl betasatellite naturally infecting pumpkin (cucurbita moschata) in india. | pumpkin cultivation in india is affected by severe incidence of a yellow vein mosaic disease. tomato leaf curl new delhi virus and squash leaf curl china virus are known to be associated with this disease in india. we were able to identify a third begomovirus-tomato leaf curl palampur virus (tolcpmv), from pumpkin showing typical symptoms of the disease at varanasi based on the sequence of complete dna-a genome of the virus. the complete dna-a sequence of the virus shared more than 99% sequence ... | 2011 | 23637491 |
| cellulase-assisted extraction of polysaccharides from cucurbita moschata and their antibacterial activity. | in this study, cellulase-assisted extraction of water soluble polysaccharides from pumpkin (cucurbita moschata) and their antibacterial activity were investigated. the polysaccharides yield was monitored during the extraction process. the optimum extraction conditions were determined as follows: time, 40 min; temperature, 55°c; ph, 4.5; and cellulase amount, 4,000 u/g. the extracts were centrifuged, filtered, proteins removed by sevag method, concentrated to approximately 15% (w/v), precipitated ... | 2014 | 24299793 |
| microplot evaluation of rootstocks for control of meloidogyne incognita on grafted tomato, muskmelon, and watermelon. | microplot experiments were conducted over two years (four growing seasons) to evaluate meloidogyne incognita resistance in rootstocks used for grafted tomato (solanum lycopersicum), muskmelon (cucumis melo), and watermelon (citrullus lanatus). three tomato rootstocks; 'tx301', 'multifort', and 'aloha', were tested in addition to the nongrafted scion, 'florida-47'. two muskmelon rootstocks; cucumis metuliferus and 'tetsukabuto' (cucurbita maxima × cucurbita moschata) were evaluated with the nongr ... | 2011 | 23431109 |
| use of cucumis metuliferus as a rootstock for melon to manage meloidogyne incognita. | root-knot nematode-susceptible melons (cantaloupe) were grown in pots with varying levels of meloidogyne incognita and were compared to susceptible melons that were grafted onto cucumis metuliferus or cucurbita moschata rootstocks. in addition, the effect of using melons as transplants in nematode-infested soil was compared to direct seeding of melons in nematode-infested soil. there were no differences in shoot or root weight, or severity of root galling between transplanted and direct-seeded n ... | 2005 | 19262873 |
| instrumental and sensory analyses of quality attributes of grafted specialty melons. | soilborne disease management remains a great challenge in melon production with the phaseout of soil fumigant methyl bromide. grafting has been shown to be an effective approach to control soilborne diseases. however, previous research has yielded mixed results regarding the impacts of rootstock on fruit quality. very few studies have assessed melon quality attributes using both sensory evaluation and instrumental methods. | 2015 | 25512001 |
| occurrence of squash yellow mild mottle virus and pepper golden mosaic virus in potential new hosts in costa rica. | leaf samples of solanum lycopersicum, capsicum annuum, cucurbita moschata, cucurbita pepo, sechium edule and erythrina spp. were collected. all samples were positive for begomoviruses using polymerase chain reaction and degenerate primers. a sequence of ∼1,100 bp was obtained from the genomic component dna-a of 14 samples. in addition, one sequence of ∼580 bp corresponding to the coat protein (av1) was obtained from a chayote (s. edule) leaf sample. the presence of squash yellow mild mottle viru ... | 2013 | 25288955 |
| chlorotic curly stunt: a severe begomovirus disease of bottle gourd in northern india. | bottle gourd (lagenaria siceraria) an important vegetable crop in india was observed to be affected by a chlorotic curly stunt disease (ccsd) during 2003-2006 in the vegetable growing areas of delhi and adjoining state of haryana. the affected plants are severely stunted and bear very small chlorotic and mildly curled leaves. incidence of the disease varied from 4.7 to 36%. the disease could be easily transmitted by whitefly, bemisia tabaci but not by sap. the causal virus was found to be a bego ... | 2010 | 23637479 |
| complete nucleotide sequence of watermelon chlorotic stunt virus originating from oman. | watermelon chlorotic stunt virus (wmcsv) is a bipartite begomovirus (genus begomovirus, family geminiviridae) that causes economic losses to cucurbits, particularly watermelon, across the middle east and north africa. recently squash (cucurbita moschata) grown in an experimental field in oman was found to display symptoms such as leaf curling, yellowing and stunting, typical of a begomovirus infection. sequence analysis of the virus isolated from squash showed 97.6-99.9% nucleotide sequence iden ... | 2012 | 22852046 |
| phloem long-distance delivery of flowering locus t (ft) to the apex. | cucurbita moschata flowering locus t-like 2 (hereafter ftl2) and arabidopsis thaliana (arabidopsis) flowering locus t (ft), components of the plant florigenic signaling system, move long-distance through the phloem from source leaves to the vegetative apex where they mediate floral induction. the mechanisms involved in long-distance trafficking of ft/ftl2 remain to be elucidated. in this study, we identified the critical motifs on both ft and ftl2 required for cell-to-cell trafficking through mu ... | 2013 | 23607279 |
| effects of root applications of gibberellic acid on photosynthesis and growth in c3 and c 4 plants. | the effects of root applications of gibberellic acid (ga3) on growth and photosynthesis of 12 species of plants including c3 monocots (triticum aestivum l., wheat, hordeum vulgare l., barley and avena sativa l., oat), c3 dicots (vigna radiata l., mung bean, cucurbita moschata l., squash and capsicum annuum l., pepper), c4 monocots (zea mays l., corn, sorghum vulgare l., sorghum and panicum ramosum l., millet) and c4 dicots (amaranthus retroflexus l., pigweed, kochia scoparis l., kochia and gomph ... | 1985 | 24442874 |
| [effects of host plants on the life table parameters of experimental populations of aphis gossypii]. | a comparative study was conducted on the life table parameters of aphis gossypii reared on five host plant species at (25 +/- 1) degrees c in laboratory. there existed significant differences in the durations of various developmental stages, adult longevity, mean offspring number per day, net reproductive rate, intrinsic rate of increase, finite rate of increase, mean generation time, and population doubling time for the a. gossypii populations reared on the host plants. for the aphids on lagena ... | 2013 | 24015567 |
| effect of reproductive mode on host plant utilization of melon aphid (hemiptera: aphididae). | variation in the reproductive mode of melon aphid aphis gossypii glover occurred on the large geographic scale, but the performance of different reproductive modes to use host plant is poorly understood. life tables of melon aphid population that undergo the anholocyclic, androcyclic, and intermediate reproductive mode were conducted on different host plants. the results showed that the anholocyclic and androcyclic strains could become adults and produce offspring on cotton gossypium hirsutum l. ... | 2010 | 22182553 |
| [entomophilic pollination of squash, cucurbita moschata (cucurbitaceae)]. | the objectives of this work were to determine the squash entomofauna in the region of viçosa, minas gerais state, to study their behavior on flowers and their importance for pollination, verifying the role of each pollinator. the most common species were trigona spinipes (fabricius), trigona hyalinata (lepeletier), apis mellifera (l.) and melipona quadrifasciata (lepeletier). the visitation behavior of a. mellifera, m. quadrifasciata, and bombus morio (swederus) were similar. they visited flower ... | 2010 | 20498949 |
| functional group diversity of bee pollinators increases crop yield. | niche complementarity is a commonly invoked mechanism underlying the positive relationship between biodiversity and ecosystem functioning, but little empirical evidence exists for complementarity among pollinator species. this study related differences in three functional traits of pollinating bees (flower height preference, daily time of flower visitation and within-flower behaviour) to the seed set of the obligate cross-pollinated pumpkin cucurbita moschata duch. ex poir. across a land-use int ... | 2008 | 18595841 |
| nutritional evaluation of phosphorylated pumpkin seed (cucurbita moschata) protein concentrate in silver catfish rhamdia quelen (quoy and gaimard, 1824). | an 8-week feeding trial was conducted to evaluate the effect of replacing fish meal with pumpkin seed meal (psm) or phosphorylated protein concentrate of pumpkin seed meal (ppcps) on growth and metabolic responses of silver catfish. five isonitrogenous and isocaloric diets were formulated. control diet contained fish meal as the main protein source. the treatment groups contained 25 and 50% of either psm or ppcps protein replaced the fishmeal protein. a total of 400 silver catfish, with initial ... | 2015 | 26377938 |
| [comparative analysis of rdna distribution in metaphase chromosomes of cucurbitaceae species]. | fluorescence in situ hybridization (fish) and double fish experiments were carried out to ascertain the chromosomal distribution patterns of the 45s and 5s ribosomal dnas in the three species of cucurbitaceae. five pairs of 45s rdna loci and two pairs of 5s rdna signals were detected on chromosomes of cucurbita moschata duch. luffa cylindrical roem. contained five pairs of 45s rdna loci and one pair of 5s rdna loci. in benincasa hispida cogn., two pairs of 45s rdna sites and one pair of 5s rdna ... | 2007 | 17548333 |
| characterization of acid-extracted pectin-enriched products obtained from red beet (beta vulgaris l. var. conditiva) and butternut (cucurbita moschata duch ex poiret). | chemical and rheological characteristics of fractions enriched in soluble dietary fiber are reported. these fractions were obtained through acid hydrolysis of butternut (cucurbita moschata duch ex poiret) and red beet (beta vulgaris l. var. conditiva) cell wall enriched powders. hydrolysis was performed using citric acid at different ph values and reaction times (2 and 3 h). yields obtained for butternut fractions were between 21 and 28 g/100 g; for red beet, yields were 24 and 31 g/100 g for ph ... | 2010 | 20178319 |
| molecular cloning and expression of a bush related cmv1 gene in tropical pumpkin. | a bush-type plant was selected from tropical pumpkin 'cga' (cucurbita moschata duchesne) in order to study the vine development in c. moschata. in this study, a novel gene encoding nadh dehydrogenase was isolated from the vine line (cgav) of c. moschata, that was not expressed in the near isogenic bush line (cgabu). this gene, designated as cmv1 (c. moschata vine 1), was 545 bp in length and was composed of a 477 bp open reading frame, which had 99% nucleotide similarity to the chloroplast ndhj ... | 2010 | 19308673 |
| [effects of celosia argentea and cucurbita moschata extracts on anti-dnp ige antibody production in mice]. | we have already reported that the perilla frutescens extract (pfe) suppressed anti-dna ige antibody production in mice. in this study, we prepared extracts of celosia argentea l. (cae) and cucurbita moschata duch (cme), which are chinese herbal medicines like perilla frutescens, and examined the effects on anti-dnp antibody responses in mice. to examine the effects of cae & cme on primary antibody responses, cae & cme were intraperitoneally injected the day before primary immunization of dnp-ova ... | 1994 | 8031259 |
| biology of anastrepha grandis (diptera: tephritidae) in different cucurbits. | anastrepha grandis (macquart) (diptera: tephritidae) is one of the main pests of cucurbits in brazil. losses occur due to the damage caused to the fruits and the embargo on exports, as a. grandis is considered a quarantine pest in countries that import brazilian cucurbits. this study aimed to evaluate the development of a. grandis in hosts of the cucurbitaceae family. the hosts used were stem squash (cucurbita pepo l.), squash (cucurbita moschata duchesne), chayote [sechium edule (jacq.) swartz] ... | 2015 | 26470226 |
| anti-hyperglycaemic globulins from selected cucurbitaceae seeds used as antidiabetic medicinal plants in africa. | the recent epidemic of diabetes mellitus (dm) in africa, coupled with rampant poverty, is an indication of the urgent need to develop new efficacious, cheaper and more available drugs to face this growing public health challenge. a number of plants products among which the protein-rich cucurbitaceae seeds are commonly used in traditional medicine with increasing acclaimed efficacy against dm. the aim of this study was to analyse and evaluate the hypoglycaemic activity of storage proteins of five ... | 2013 | 23506532 |
| ethylene-induced overproduction of reactive oxygen species is responsible for the development of watersoaking in immature cucumber fruit. | watersoaking is an ethylene-induced disorder observed in some members of the cucurbitaceae including cucumber (cucumis sativus l.), watermelon (citrullus lanatus thunb. matsum and nakai), and tropical pumpkin (cucurbita moschata duch.). previous studies have found that immature beit-alpha cucumber (cv. manar) exhibit watersoaking after 6d of continuous exposure to 10 μll(-1) ethylene in air (21 kpa o(2)). the present study was designed to investigate the early dynamics of ethylene responses in i ... | 2013 | 22999588 |
| characterization of the small rna component of leaves and fruits from four different cucurbit species. | micrornas (mirnas) are a class of non-coding small rnas involved in post-transcriptional regulation of gene expression critical for plant growth and development, stress responses and other diverse biological processes in plants. the cucurbitaceae or cucurbit family represents some of economically important species, particularly those with edible and medicinal fruits. genomic tools for the molecular analysis of members of this family are just emerging. partial draft genome sequence became availab ... | 2012 | 22823569 |
| analysis of gamma-irradiated melon, pumpkin, and sunflower seeds by electron paramagnetic resonance spectroscopy and gas chromatography-mass spectrometry. | seeds of melon (citrullus lanatus var. sp.), pumpkin (cucurbita moschata), and sunflower (heliantus annus) were gamma-irradiated at 1, 3, 5, and 10 kgy and analyzed by electron paramagnetic resonance (epr) and gas chromatography-mass spectrometry (gc-ms) according to en1787:2000 and en1785:2003, respectively. distinguishable triplet signals due to the presence of induced cellulose radicals were found at 2.0010-2.0047 g in the epr spectra. the gamma-irradiated radiolytic markers of 2-dodecylcyclo ... | 2006 | 16968077 |
| isolation and functional characterization of cslsi1, a silicon transporter gene in cucumis sativus. | cucumber (cucumis sativus) is a widely grown cucurbitaceous vegetable that exhibits a relatively high capacity for silicon (si) accumulation, but the molecular mechanism for silicon uptake remains to be clarified. here we isolated and characterized cslsi1, a gene encoding a silicon transporter in cucumber (cv. mch-4). cslsi1 shares 55.70 and 90.63% homology with the lsi1s of a monocot and dicot, rice (oryza sativa) and pumpkin (cucurbita moschata), respectively. cslsi1 was predominantly expresse ... | 2017 | 27701737 |
| can adverse effects of acidity and aluminum toxicity be alleviated by appropriate rootstock selection in cucumber? | low-ph and aluminum (al) stresses are the major constraints that limit crop yield in acidic soils. grafting vegetable elite cultivars onto appropriate rootstocks may represent an effective tool to improve crop tolerance to acidity and al toxicity. two greenhouse hydroponic experiments were performed to evaluate growth, yield, biomass production, chlorophyll index, electrolyte leakage, mineral composition, and assimilate partitioning in plant tissues of cucumber plants (cucumis sativus l. "ekron" ... | 2016 | 27621740 |
| cucumber (cucumis sativus l.) and kabocha squash (cucurbita moschata duch). | we established improved methods for agrobacterium-mediated transformation of cucumber (cucumis sativus l.) and kabocha squash (cucurbita moschata duch). vacuum infiltration of cotyledonary explants with agrobacterium suspension enhanced the agrobacterium infection efficiency in the proximal regions of explants. wounding treatment was also essential for kabocha squash. cocultivation on filter paper wicks suppressed necrosis of explants, keeping regeneration efficacy. putative transgenic plants we ... | 2015 | 25300850 |
| grafting-responsive mirnas in cucumber and pumpkin seedlings identified by high-throughput sequencing at whole genome level. | grafting is an important agricultural technique widely used for improving growth, yields and tolerance of crops to abiotic and biotic stresses. as one type of endogenous, non-coding small rnas, micrornas (mirnas) regulate development and responsiveness to biotic and abiotic stresses by negatively mediating expression of target genes at the post-transcriptional level. however, there have been few detailed studies to evaluate the role of mirnas in mediation of grafting-induced physiological proces ... | 2014 | 24279842 |
| cucurbita spp. and cucumis sativus enhance the dissipation of polychlorinated biphenyl congeners by stimulating soil microbial community development. | a number of cucurbita species have the potential to extract polychlorinated biphenyls (pcbs) from soil, but their impact on the soil microbial communities responsible for pcb degradation remains unclear. a greenhouse experiment was conducted to investigate the effect of three cucurbita and one cucumis species on pcb dissipation and soil microbial community structure. compared to the unplanted control, enhanced losses of pcbs (19.5%-42.7%) were observed in all planted soils. cucurbita pepo and cu ... | 2014 | 24077568 |
| isolation and functional characterization of an influx silicon transporter in two pumpkin cultivars contrasting in silicon accumulation. | a high accumulation of silicon (si) is required for overcoming abiotic and biotic stresses, but the molecular mechanisms of si uptake, especially in dicotyledonous species, is poorly understood. herein, we report the identification of an influx transporter of si in two cucurbita moschata (pumpkin) cultivars greatly differing in si accumulation, which are used for the rootstocks of bloom and bloomless cucumis sativus (cucumber), respectively. heterogeneous expression in both xenopus oocytes and r ... | 2011 | 21205032 |
| [relationships of aulacophora beetles feeding behavior with cucurbitacin types in host crops]. | aulacophora fermoralis chinensis and aulacophora cattigarensis are the two related aulacophora beetles, but their host selectivity and feeding behavior are significantly different. a. fernzoralis chinensis usually feeds upon cucumis sativus and cucurbita moschata, but never upon luffa acutangula, momordica charantia and citrullas lanatus. its feeding behavior on hosts is to snip a circular trench on their leaves, and then, to feed the leaf tissues isolated by the trench. on the contrary, a. catt ... | 2005 | 16252877 |
| plant exudates trigger leaf-trenching by cabbage loopers, trichoplusia ni (noctuidae). | cabbage loopers, trichoplusia ni, cut a narrow trench across leaves of plants that release exudate, then feed distal to the trench in an area of reduced exudation. the larvae do not normally trench plant species such as plantain, plantago lanceolata, that lack exudate. to determine what cues elicit trenching, i reared larvae to the final instar on plantain, then applied test solutions to their mouthparts during feeding. loopers that received latex from lactuca serriola (asteraceae) or phloem exu ... | 1997 | 28307485 |
| reassessing coxcatlan cave and the early history of domesticated plants in mesoamerica. | reanalysis and direct accelerator mass spectrometry radiocarbon dating of the cucurbit assemblage from coxcatlan cave provide information on the timing and sequence of the initial appearance of three domesticated plants in the tehuacán valley (puebla, mexico) and allow reassessment of the overall temporal context of plant domestication in mexico. cucurbita pepo is the earliest documented domesticate in the cave, dating to 7,920 calibrated calendrical (cal) years b.p. the bottle gourd (lagenaria ... | 2005 | 15908505 |
| phylogenetic relationships among domesticated and wild species of cucurbita (cucurbitaceae) inferred from a mitochondrial gene: implications for crop plant evolution and areas of origin. | we have investigated the phylogenetic relationships among six wild and six domesticated taxa of cucurbita using as a marker an intron region from the mitochondrial nad1 gene. our study represents one of the first successful uses of a mtdna gene in resolving inter- and intraspecific taxonomic relationships in angiosperms and yields several important insights into the origins of domesticated cucurbita. first, our data suggest at least six independent domestication events from distinct wild ancesto ... | 2002 | 11782554 |
| sequence variations in the fad2 gene in seeded pumpkins. | seeded pumpkins are important economic crops; the seeds contain various unsaturated fatty acids, such as oleic acid and linoleic acid, which are crucial for human and animal nutrition. the fatty acid desaturase-2 (fad2) gene encodes delta-12 desaturase, which converts oleic acid to linoleic acid. however, little is known about sequence variations in fad2 in seeded pumpkins. twenty-seven fad2 clones from 27 accessions of cucurbita moschata, cucurbita maxima, cucurbita pepo, and cucurbita ficifoli ... | 2015 | 26782391 |
| high throughput sequencing of small rnas in the two cucurbita germplasm with different sodium accumulation patterns identifies novel micrornas involved in salt stress response. | micrornas (mirnas), a class of small non-coding rnas, recognize their mrna targets based on perfect sequence complementarity. mirnas lead to broader changes in gene expression after plants are exposed to stress. high-throughput sequencing is an effective method to identify and profile small rna populations in non-model plants under salt stresses, significantly improving our knowledge regarding mirna functions in salt tolerance. cucurbits are sensitive to soil salinity, and the cucurbita genus is ... | 2015 | 26010449 |
| effects of grafting with pumpkin rootstock on carbohydrate metabolism in cucumber seedlings under ca(no3)2 stress. | this study investigated the effects of grafting on the carbohydrate status and the enzymes of carbohydrate metabolism in self-grafted and grafted cucumber seedlings using the salt-tolerant pumpkin rootstock 'qingzhen 1' (cucurbita maxima × cucurbita moschata) under 80 mm ca(no3)2 stress for 6 d. the growth of self-grafted seedlings was significantly inhibited after the treatment of ca(no3)2 stress, whereas the inhibition of growth was alleviated in pumpkin rootstock-grafted seedlings. ca(no3)2 s ... | 2015 | 25579659 |
| antioxidant enzymatic activities and gene expression associated with heat tolerance in the stems and roots of two cucurbit species ("cucurbita maxima" and "cucurbita moschata") and their interspecific inbred line "maxchata". | the elucidation of heat tolerance mechanisms is required to combat the challenges of global warming. this study aimed to determine the antioxidant enzyme responses to heat stress, at the enzymatic activity and gene expression levels, and to investigate the antioxidative alterations associated with heat tolerance in the stems and roots of squashes using three genotypes differing in heat tolerance. plants of heat-tolerant "c. moschata", thermolabile "c. maxima" and moderately heat-tolerant intersp ... | 2013 | 24336062 |
| characteristics of organic acids in the fruit of different pumpkin species. | the aim of the research was to determine the composition of organic acids in fruit of different cultivars of three pumpkin species. the amount of acids immediately after fruit harvest and after 3 months of storage was compared. the content of organic acids in the examined pumpkin cultivars was assayed using the method of high performance liquid chromatography (hplc). three organic acids (citric acid, malic acid, and fumaric acid) were identified in the cultivars, whose content considerably varie ... | 2014 | 24262577 |
| carotenoid accumulation and carotenogenic gene expression during fruit development in novel interspecific inbred squash lines and their parents. | carotenoid levels and composition during squash fruit development were compared in cucurbita moschata , cucurbita maxima , and two lines of their interspecific inbred lines, namely, maxchata1 and maxchata2. eight genes associated with carotenoid biosynthesis were analyzed by quantitative rt-pcr. the two squash species and their interspecific inbred lines exhibited different qualitative and quantitative carotenoid profiles and regulatory mechanisms. c. moschata had the lowest total carotenoid con ... | 2012 | 22574777 |
| changes in carotenoids during processing and storage of pumpkin puree. | changes in the contents of carotenoids and their true retentions (% tr) during the production of puree of cucurbita moschata 'menina brasileira' and of cucurbita maxima 'exposição' pumpkins and the stability of such compounds during 180days of storage were monitored by liquid chromatography coupled with a photodiode array detector. cooking caused higher losses than commercial sterilisation. high losses of xanthophylls such as lutein and violaxanthin were noted during processing and storage of pu ... | 2011 | 25214348 |
| evaluation of alternatives to methyl bromide in melon crops in guatemala. | the monoculture of melon in guatemala has caused the massive appearance of plants with an analogous syndrome for the well-known disease commonly called melon collapse, or vine decline, causing significant losses in crops. methyl bromide is commonly used to sterilize soil prior to planting in guatemala, but it must be phased out by 2015. the objective of this study was to evaluate the technique of grafting melon onto hybrids of cucurbita (cucurbita maxima x cucurbita moschata), as an alternative ... | 2009 | 18998189 |
| hplc-dad-ms(n) characterisation of carotenoids from apricots and pumpkins for the evaluation of fruit product authenticity. | carotenoids including carotenoid esters from six apricot (prunus armeniaca l.) cultivars and from eight cultivars from three pumpkin species (cucurbita maxima duch., cucurbita pepo l., and cucurbita moschata duch.) were extracted without saponification, separated on a c-30 reversed-phase column and characterised by high-performance liquid chromatography/atmospheric pressure chemical ionisation-mass spectrometry (lc-ms). the predominant free carotenoids were quantified by hplc with diode array de ... | 2008 | 26049248 |
| qualitative and quantitative differences in carotenoid composition among cucurbita moschata, cucurbita maxima, and cucurbita pepo. | squashes and pumpkins are important dietary sources of carotenoids worldwide. the carotenoid composition has been determined, but reported data have been highly variable, both qualitatively and quantitatively. in the present work, the carotenoid composition of squashes and pumpkins currently marketed in campinas, brazil, were determined by hplc-dad, complemented by hplc-ms for identification. cucurbita moschata 'menina brasileira' and c. moschata 'goianinha' had similar profiles, with beta-carot ... | 2007 | 17444652 |
| phenolic profiles of raw apricots, pumpkins, and their purees in the evaluation of apricot nectar and jam authenticity. | the possibility of proving the undeclared addition of pumpkin puree in apricot nectars and jams has been investigated by using the phenol compound fingerprint and sensory evaluation. the cheaper pumpkin admixtures in apricot nectars and jams could not be detected by the sensory evaluation, particularly if present in quantities of <15%. the lower admixtures of pumpkin puree in apricot nectars and jams could be detected by the presence of syringic acid, a phenolic compound characteristic of the in ... | 2005 | 15941324 |
| cultivar-based introgression mapping reveals wild species-derived pm-0, the major powdery mildew resistance locus in squash. | powdery mildew is a major fungal disease on squash and pumpkin (cucurbita spp.) in the us and throughout the world. genetic resistance to the disease is not known to occur naturally within cucurbita pepo and only infrequently in cucurbita moschata, but has been achieved in both species through the introgression of a major resistance gene from the wild species cucurbita okeechobeensis subsp. martinezii. at present, this gene, pm-0, is used extensively in breeding, and is found in nearly all powde ... | 2016 | 27936008 |
| carboxylesterase-involved metabolism of di-n-butyl phthalate in pumpkin (cucurbita moschata) seedlings. | uptake and accumulation by plants is a significant pathway in the migration and transformation of phthalate esters (paes) in the environment. however, limited information is available on the mechanisms of pae metabolism in plants. here, we investigated the metabolism of di-n-butyl phthalate (dnbp), one of the most frequently detected paes, in pumpkin (cucurbita moschata) seedlings via a series of hydroponic experiments with an initial concentration of 10 mg l(-1). dnbp hydrolysis occurred primar ... | 2017 | 27697378 |
| combined infrared-vacuum drying of pumpkin slices. | infrared-vacuum dehydration characteristics of pumpkin (cucurbita moschata) were evaluated in a combined dryer system. the effects of drying parameters, infrared radiation power (204-272 w), system pressure (5-15 kpa), slice thickness (5 and 7 mm) and time (0-220 min) on the drying kinetics and characteristics of pumpkin slices were investigated. the vacuum pressure, lamp power and slice had significant effect on the drying kinetics and various qualities of the dried pumpkin. moisture ratios wer ... | 2016 | 27407204 |
| cucurbita moschata duch. and its active component, β-carotene effectively promote the immune responses through the activation of splenocytes and macrophages. | cucurbita moschata duch. has long been used for traditional health food in many countries. however, to enhance the immune system of cucurbita moschata duch. and its major component, β-carotene is not clear. here, we determined the immune enhancement effect of cucurbita moschata duch. and β-carotene in mouse splenocytes and raw 264.7 macrophage cell line. we prepared baked cucurbita moschata duch. (sweetme sweet pumpkin(tm), ssp) and steamed cucurbita moschata duch. (sc). splenocytes isolated fro ... | 2016 | 27315229 |
| biodegradation of γ-hexachlorocyclohexane by transgenic hairy root cultures of cucurbita moschata that accumulate recombinant bacterial lina. | γ-hch was successfully degraded using lina-expressed transgenic hairy root cultures of cucurbita moschata . fusing an endoplasmic reticulum-targeting signal peptide to lina was essential for stable accumulation in the hairy roots. the pesticide γ-hexachlorocyclohexane (γ-hch) is a persistent organic pollutant (pop) that raises public health and environmental pollution concerns worldwide. although several isolates of γ-hch-degrading bacteria are available, inoculating them directly into γ-hch-con ... | 2016 | 27295266 |
| improvement of depressive behavior by sweetme sweet pumpkin™ and its active compound, β-carotene. | sweetme sweet pumpkin™ (ssp, baked cucurbita moschata duch.) has been used to treat patients with depression in korea. however, the role of ssp in improving depression has not been elucidated yet. thus, we assessed the antidepressant-like effect of ssp and its active compound, β-carotene, with the forced swimming test (fst). | 2016 | 26820672 |
| α-cyclodextrin encapsulation of supercritical co₂ extracted oleoresins from different plant matrices: a stability study. | here we describe the encapsulation in α-cyclodextrins (α-cds) of wheat bran, pumpkin and tomato oleoresins, extracted by supercritical carbon dioxide, to obtain freeze-dried powders useful as ready-to-mix ingredients for novel functional food formulation. the stability of tocochromanols, carotenoids and fatty acids in the oleoresin/α-cd complexes, compared to the corresponding free oleoresins, was also monitored over time in different combinations of storage conditions. regardless of light, stor ... | 2016 | 26776025 |
| effect of different drying methods on moisture ratio and rehydration of pumpkin slices. | this study was carried to determine the influence of hot air drying process and combined methods on physicochemical properties of pumpkin (cucurbita moschata) samples. the experiments in hot air chamber were lead at 50, 60 and 70 °c. the combined method consists of a triple combination of the main drying techniques. thus, in first stage the samples were dried in hot air convection at 60 °c followed by hot air ventilation at 40 °c simultaneous with microwave. the time required to reduce the moist ... | 2016 | 26575719 |
| dehydrodiconiferyl alcohol (dhca) modulates the differentiation of th17 and th1 cells and suppresses experimental autoimmune encephalomyelitis. | dehydrodiconiferyl alcohol (dhca), originally isolated from the stems of cucurbita moschata, has previously been shown to exhibit anti-adipogenic and anti-lipogenic effects in 3t3-l1 cells and primary mouse embryonic fibroblasts (mefs) (lee et al., 2012). here, we investigated whether synthetic dhca could suppress the cd4 t helper 17 (th17)-mediated production of the interleukin (il)-17 protein. the results from rt-qpcr suggest that dhca-mediated down-regulation of il-17 occurred at the transcri ... | 2015 | 26477735 |
| α-carotene and β-carotene content in raw and cooked pulp of three mature stage winter squash "type butternut". | winter squash "type butternut" is harvested in physiological ripening for better commercial distribution, when sensory and/or nutritional quality is not optimum for consumption. the objective of this study was to quantify the content of α-carotene, β-carotene, color and dry matter in the pulp of raw and microwave-cooked winter squash "type butternut" (variety cosmof1) in three states of commercial maturity. immature, mature, and very mature fruit, defined at the time of the harvest by the percen ... | 2015 | 28231218 |
| subcellular distribution and uptake mechanism of di-n-butyl phthalate in roots of pumpkin (cucurbita moschata) seedlings. | phthalate acid esters (paes) are of particular concern due to their potential environmental risk to human and nonhuman organisms. although uptake of paes by plants has been reported by several researchers, information about the intracellular distribution and uptake mechanisms of paes is still lacking. in this study, a series of hydroponic experiments using intact pumpkin (cucurbita moschata) seedlings was conducted to investigate how di-n-butyl phthalate (dnbp), one of the most frequently identi ... | 2016 | 26304812 |
| a novel aqueous two phase system composed of surfactant and xylitol for the purification of lipase from pumpkin (cucurbita moschata) seeds and recycling of phase components. | lipase is one of the more important enzymes used in various industries such as the food, detergent, pharmaceutical, textile, and pulp and paper sectors. a novel aqueous two-phase system composed of surfactant and xylitol was employed for the first time to purify lipase from cucurbita moschata. the influence of different parameters such as type and concentration of surfactants, and the composition of the surfactant/xylitol mixtures on the partitioning behavior and recovery of lipase was investiga ... | 2015 | 26091076 |
| effective suppression of pro-inflammatory molecules by dhca via ikk-nf-κb pathway, in vitro and in vivo. | dehydrodiconiferyl alcohol (dhca), a lignan compound isolated from cucurbita moschata, has previously been shown to contain anti-adipogenic and antilipogenic effects on 3t3-l1 cells and mouse embryonic fibroblasts. as some of phytochemicals derived from natural plants show anti-inflammatory or antioxidative activities, we determined whether dhca affects the production of pro-inflammatory mediators and also investigated its underlying mechanisms. | 2015 | 25802070 |
| upregulation of heme oxygenase-1 expression by dehydrodiconiferyl alcohol (dhca) through the ampk-nrf2 dependent pathway. | oxidative stress is induced by the accumulation of free radicals, resulting in an imbalanced cellular redox state, which has been implicated in a variety of human diseases. dehydrodiconiferyl alcohol (dhca), a lignan compound isolated from cucurbita moschata, has previously been reported to contain anti-adipogenic and anti-lipogenic effects on 3t3-l1 cells and primary mefs (abraham and kappas, 2008). in this study, it was tested whether dhca could affect the expression of ho-1, using raw264.7 mo ... | 2014 | 25261737 |
| ultrasound-assisted enzymatic extraction and antioxidant activity of polysaccharides from pumpkin (cucurbita moschata). | an efficient ultrasound-assisted enzymatic extraction (uaee) of cucurbita moschata polysaccharides (cmcp) was established and the cmcp antioxidant activities were studied. the uaee operating parameters (extraction temperature, ultrasonic power, ph, and liquid-to-material ratio) were optimized using the central composite design (ccd) and the mass transfer kinetic study in uaee procedure was used to select the optimal extraction time. enzymolysis and ultrasonication that were simultaneously conduc ... | 2014 | 25256490 |
| effects of season and storage period on accumulation of individual carotenoids in pumpkin flesh (cucurbita moschata). | carotenoids are antioxidants with pharmaceutical potential. the major carotenoids important to humans are α-carotene, β-carotene, lycopene, lutein, zeaxanthin, and β-cryptoxanthin. some of the biological functions and actions of these individual carotenoids are quite similar to each other, whereas others are specific. besides genotype and location, other environmental effects such as temperature, light, mineral uptake, and ph have been found affect carotenoid development in plant tissues and org ... | 2014 | 25007748 |
| performance of the striped mealybug ferrisia virgata cockerell (hemiptera: pseudococcidae) under variable conditions of temperature and mating. | mealybugs have strong associations with their host plants due to their limitations for dispersal. thus, environmental conditions and host quality may impact the biological traits of mealybugs. to the best of our knowledge, we are the first to report on the biology of a brazilian population of the striped mealybug ferrisia virgata cockerell (hemiptera: pseudococcidae), which has recently been reported to infest cotton in brazil. we evaluated the development and reproductive performance of f. virg ... | 2014 | 27193398 |
| hypolipidemic effect of the polysaccharides extracted from pumpkin by cellulase-assisted method on mice. | the fruit of pumpkin (cucurbita moschata) is one of the most important vegetables in the world. this study was conducted to investigate the hypolipidemic effect of the polysaccharide isolated from pumpkin (pp). male sprague-dawley rats were divided into three groups and treated with diets containing either high fat, pp, or normal fat. oral administration of pp could significantly decrease the levels of plasma triacylglycerol (tg), total cholesterol (tc), and plasma low-density lipoprotein choles ... | 2014 | 24325857 |
| effect of drying and co-matrix addition on the yield and quality of supercritical co₂ extracted pumpkin (cucurbita moschata duch.) oil. | in this work a process for obtaining high vitamin e and carotenoid yields by supercritical carbon dioxide (sc-co₂) extraction from pumpkin (cucurbita moschata duch.) is described. the results show that the use of a vacuum oven-dried [residual moisture (∼8%)] and milled (70 mesh sieve) pumpkin flesh matrix increased sc-co₂ extraction yields of total vitamin e and carotenoids of ∼12.0- and ∼8.5-fold, respectively, with respect to the use of a freeze-dried and milled flesh matrix. the addition of m ... | 2014 | 24262563 |
| effect of gamma irradiation and storage time on microbial growth and physicochemical characteristics of pumpkin (cucurbita moschata duchesne ex poiret) puree. | the effect of gamma irradiation (0-2 kgy) and storage time (0-28 days) on microbial growth and physicochemical characteristics of a packed pumpkin puree was studied. for that purpose, a factorial design was applied. the puree contained potassium sorbate, glucose and vanillin was stored at 25°c . gamma irradiation diminished and storage time increased microbial growth. a synergistic effect between both variables on microbial growth was observed. storage time decreased ph and color of purees. sorb ... | 2014 | 23733817 |
| modeling and optimization of ultrasound-assisted extraction of polysaccharide from cucurbita moschata. | polysaccharides from pumpkin were extracted by ultrasound-assisted extraction technology using four factors at five levels central composite rotatable response surface design (ccrd). on using single factor analysis, process variables such as extraction temperature (50-70 °c), power of ultrasound (50-70 w), time (15-25 min) and solid-liquid ratio (1:10-1:20 g/ml) were selected. experiments were conducted to evaluate the effects of four independent variables on the maximum extraction yield of poly ... | 2013 | 23399253 |
| phytochemicals and antioxidative enzymes defence mechanism on occurrence of yellow vein mosaic disease of pumpkin (cucurbita moschata). | pumpkin (cucurbita moschata) samples showing yellow vein mosaic disease in varanasi region were identified with begomovirus infection using pcr amplification. a sequencing analysis of the full genome revealed that it is a strain of tomato leaf curl palampur virus (genbank id. fj931537). phytochemical composition and antioxidative enzyme levels were compared in infected and healthy plants. the study revealed that the amount of total protein declined in the infected leaves but elevated up to 135 % ... | 2013 | 28324587 |
| pumpkin (cucurbita moschata) fruit extract improves physical fatigue and exercise performance in mice. | pumpkin (cucurbita moschata) is a popular and nutritious vegetable consumed worldwide. the overall purpose of this study was to evaluate the effects of c. moschata fruit extract (cme) on anti-fatigue and ergogenic functions following physiological challenges. male icr mice from four groups designated vehicle, cme-50, cme-100 and cme-250, respectively (n = 8 per group in each test) were orally administered cme for 14 days at 0, 50, 100 and 250 mg/kg/day. the anti-fatigue activity and exercise per ... | 2012 | 23047485 |
| bioactive compounds in lipid fractions of pumpkin (cucurbita sp) seeds for use in food. | seeds are considered to be agro-industrial residues, which can be used as source of macronutrients and/or raw material for extraction of vegetable oils, since they present great quantities of bioactive compounds. this study aimed to characterize the lipid fractions and the seeds of pumpkin (cucurbita sp) varieties nova caravela, mini paulista, menina brasileira, and moranga de mesa aiming at using them in food. the chemical composition of the seeds was performed according to the official methods ... | 2012 | 22671521 |