| purification of ent-kaurene oxidase from gibberella fujikuroi and cucurbita maxima. | | 1992 | 1397591 |
| screening of the antimalarial activity of plants of the cucurbitaceae family. | crude ethanolic extracts (cees) from two species of cucurbitaceae, cucurbita maxima and momordica charantia (commonly called "abóbora moranga" and "melão de são caetano", respectively) were assayed for antimalarial activity by the 4-d suppressive test. the cee of dry c. maxima seeds showed strong antimalarial activity following oral administration (250 and 500 mg/kg), reducing by 50% the levels of parasitemia in plasmodium berghei-infected mice. treatment of normal animals with 500 mg/kg of the ... | 1991 | 1841996 |
| inhibition of human beta-factor xiia by squash family serine proteinase inhibitors. | many inhibitors of trypsin and human beta-factor xiia have been isolated from squash and related seeds and sequenced (wieczorek et al., biochem. biophys. res. comm. (1985) 126, 646-652). the association equilibrium constants (ka) of several of these inhibitors have now been determined with human beta-factor xiia using a modification of the method of green and work (park et al., fed. proc. fed. am. soc. exp. biol. (1984) 43, 1962). the ka's range from 7.8 x 10(4) m-1 to 3.3 x 10(8) m-1. two isoin ... | 1990 | 2306254 |
| [characterization of xanthomonas campestris pv. cucurbitae, causal agent of the bacterial spot of squash]. | bacterial leaf spot of squash was characterized for the first time in argentina. cultural, physiological, morphological and cross-infection tests on cucurbitaceae showed that the pathogen was xanthomonas campestris pv. cucurbitae (bryan) dye. the bacterium isolated from winter squash proved pathogenic for pumpkin, winter squash, cucumber and watermelon but no for muskmelon. | 1989 | 2748850 |
| two linear plasmids in mitochondria of fusarium solani f. sp. cucurbitae. | two linear plasmid-like dnas designated pfsc1 (9.2 kbp) and pfsc2 (8.3 kbp) were found in an isolate of the plant pathogenic fungus fusarium solani f. sp. cucurbitae race 1. the plasmids were maternally inherited and copurified with mitochondrial dna obtained from a mitochondria-enriched cell fraction suggesting that they are located in mitochondria. the plasmids did not share extensive sequence similarity. no homology was detected between either plasmid and the nuclear or mitochondrial genome w ... | 1988 | 3399553 |
| p protein in the phloem of cucurbita. i. the development of p-protein bodies. | light and electron microscopical observations of the cells of the phloem of cucurbita maxima have shown that two distinct types of p-protein bodies are formed: a larger type which arises as fine fibrils and a smaller type which apparently arises as groups of tubules. the tubules of the smaller type of body measure 242 +/- 3.6 (se) a (n = 48) and appear morphologically identical with the p1-protein tubules of nicotiana tabacum l. in some of these p1-protein bodies the tubules are arranged in a re ... | 1968 | 5675415 |
| anthelmintic activity of cucurbita maxima (kaddu) seeds. | | 1967 | 6076370 |
| identification of sulphur-rich proteins which resist rumen degradation and are hydrolysed rapidly by intestinal proteases. | several proteins with high proportions of s-containing essential amino acids were incubated in sheep rumen fluid in vitro and their rate of digestion was examined by sodium dodecyl sulphate-polyacrylamide-gel electrophoresis. the s-rich proteins rice prolamin (10 kda), maize zein (10 kda) and the 3.2 kda pumpkin (cucurbita maxima l.) trypsin inhibitor-1 (cmti-1) were highly resistant to rumen fluid degradation, relative to control proteins of known degradation rate (casein, bovine serum albumin ... | 1994 | 7827006 |
| toxicologic evaluation of acute and subacute oral administration of cucurbita maxima seed extracts to rats and swine. | the extract prepared from dried seeds of cucurbita maxima was administered to rats and pigs. following a single dose or 4 weeks of daily oral administration, the extract produced no changes in serum glucose, urea, creatinine, total protein, uric acid, got, gpt, ldh or blood counts. urine analysis (urea, uric acid, creatinine, total protein, na and k), as well as histopathological investigation, showed no abnormalities. these results taken as a whole indicate that the seeds of c. maxima as used i ... | 1994 | 7967649 |
| analogues of cucurbita maxima trypsin inhibitor iii (cmti-iii) with elastase inhibitory activity. | three new cmti-iii analogues containing the val residue in the reactive site (position 5) were synthesized by the solid-phase method. the analogues displayed an elastase inhibitory activity. it is shown that the removal of the n-terminal arg residue and the introduction of the gly-pro-gln tripeptide in the region 23-25 decreases the antielastase activity by two orders of magnitude. the removal of the disulfide bridge in positions 16-28 and the substitution of ala for cys16 and gly for cys28 decr ... | 1994 | 8060538 |
| antigenotoxic spinasterol from cucurbita maxima flowers. | the antigenotoxic constituent of squash flowers was isolated by solvent partitioning and repeated vacuum liquid chromatography. the micronucleus test, an in vivo method, was used to monitor the antigenotoxicity of the various fractions during the isolation process. isolate sqfwb2d from the chloroform extract of squash flowers is the most antigenotoxic isolate. it decreased the mutagenicity of tetracycline by 64.7% at a dosage of 100 mg/kg mouse. statistical analysis using kruskall wallis one-way ... | 1996 | 8649469 |
| molecular cloning and photoperiod-regulated expression of gibberellin 20-oxidase from the long-day plant spinach. | spinach (spinacia oleracea l.) is a long-day (ld) rosette plant in which stem growth under ld conditions is mediated by gibberellins (gas). major control points in spinach are the later steps of sequential oxidation and elimination of c-20 of c20-gas. degenerate oligonucleotide primers were used to obtain a polymerase chain reaction product from spinach genomic dna that has a high homology with ga 20-oxidase cdnas from cucurbita maxima l. and arabidopsis thaliana heynh. this polymerase chain rea ... | 1996 | 8742334 |
| synthesis of a squash-type protease inhibitor by gene engineering and effects of replacements of conserved hydrophobic amino acid residues on its inhibitory activity. | cucurbita maxima trypsin inhibitor i (cmti-i), a member of the squash-type protease inhibitor family, is composed of 29 amino acids and shows strong inhibition of trypsin by its compact structure. to study the structure-function relationship of this inhibitor using protein engineering methods, we constructed an expression system for cmti-i as a fused protein with porcine adenylate kinase (adk). a met residue was introduced into the junction of adk and cmti-i to cleave the fusion protein with cnb ... | 1996 | 9010939 |
| effect of disruption of a cutinase gene (cuta) on virulence and tissue specificity of fusarium solani f. sp. cucurbitae race 2 toward cucurbita maxima and c. moschata. | a 3.9-kb genomic dna fragment from the cucurbit pathogen fusarium solani f. sp. cucurbitae race 2 was cloned. sequence analysis revealed an open reading frame of 690 nucleotides interrupted by a single 51-bp intron. the nucleotide and predicted amino acid sequences showed 92 and 98% identity, respectively, to those of the cuta gene of the pea pathogen f. solani f. sp. pisi. a gene replacement vector was constructed and used to generate cuta- mutants that were detected with a polymerase chain rea ... | 1997 | 9100380 |
| solution structure by nmr of circulin a: a macrocyclic knotted peptide having anti-hiv activity. | the three-dimensional solution structure of circulin a, a 30 residue polypeptide from the african plant chassalia parvifolia, has been determined using two-dimensional 1h-nmr spectroscopy. circulin a was originally identified based upon its inhibition of the cytopathic effects and replication of the human immunodeficiency virus. structural restraints consisting of 369 interproton distances inferred from nuclear overhauser effects, and 21 backbone dihedral and nine chi1 angle restraints from spin ... | 1999 | 9878410 |
| purification and partial characterization of the pancreatic proteolytic enzymes trypsin, chymotrypsin, and elastase from the chicken. | the purpose of this work was to isolate, purify and partially sequence trypsin, chymotrypsin and elastase from the chicken pancreas. the extraction of the pancreatic zymogens with 0.5 m cacl2 at ph 7.5 for 9 h appeared to be most effective in obtaining maximum recovery of the three enzymes. the sequential cucurbita maxima trypsin inhibitor i/bovine pancreas trypsin inhibitor/soybean trypsin inhibitor affinity chromatography gave the best result for the isolation of trypsin, chymotrypsin and elas ... | 1999 | 10480246 |
| identification and localization of transformed cells in agrobacterium tumefaciens-induced plant tumors | agrobacterium tumefaciens-induced tumors of dicotyledonous plants consist of well-defined vascular bundle-like structures originating from transformed cells. the current view that 25% of the tumor cells are transformed has been re-investigated by using beta-glucuronidase (gus)-gene-containing wild-type bacteria (a281 p35s gus-int). regularly growing stem and leaf tumors showed irregular gus-staining patterns in the different plant species, ricinus communis l., cucurbita maxima l., vicia faba l. ... | 1999 | 10550620 |
| traditional antihelmintic, antiparasitic and repellent uses of plants in central italy. | the uses of 51 plants of marche, abruzzo and latium, distributed in 28 families, are listed here. memories and news of continued use of the plants in these sectors were collected from farmers and shepherds in person (mostly old people). the plants most frequently used as antiparasitics and repellents are juglans regia, lupinus albus, ruta graveolens, fraxinus ornus, datura stramonium, artemisia absinthium, allium cepa, while r. graveolens, cucurbita maxima, a. absinthium, allium sativum are the ... | 1999 | 10624877 |
| induction of cutinolytic esterase activity during saprophytic growth of cucurbit pathogens, fusarium solani f. sp. cucurbitae races one and two (nectria haematococca mpi and mpv, respectively). | cutins from fruit of cucurbita maxima and cucurbita moschata cultivars, apple and a c(16) alcohol (hexadecanol) were used to induce cutinolytic esterase activity during saprophytic growth of strains of the two cucurbit pathogens, fusarium solani f. sp. cucurbitae, race 1 (nectria haematococca mating population (mpi) and f. solani f. sp. cucurbitae, race 2 (mpv). four strains of mpv and 11 strains of mpi were were included in the study. although we were primarily interested in the two cucurbit pa ... | 2001 | 11164297 |
| high-resolution structures of three new trypsin-squash-inhibitor complexes: a detailed comparison with other trypsins and their complexes. | an anionic trypsin from atlantic salmon and bovine trypsin have been complexed with the squash-seed inhibitors, cmti-i (cucurbita maxima trypsin inhibitor i, p1 arg) and cpti-ii (cucurbita pepo trypsin inhibitor ii, p1 lys). the crystal structures of three such complexes have been determined to 1.5-1.8 a resolution and refined to crystallographic r factors ranging from 17.6 to 19.3%. the two anionic salmon-trypsin complexes (st-cpti and st-cmti) and the bovine-trypsin complex (bt-cpti) have been ... | 1999 | 10089404 |
| expression, refolding, and activation of the catalytic domain of human blood coagulation factor xii. | human blood coagulation factor xii (fxii; 80 kda) contains a c-terminal serine protease zymogen domain, which becomes activated upon contacting a negative surface. activated fxii (alphafxiia) brings about reciprocal activation of fxii and kallikrein that by further hydrolysis produces the free catalytic domain (betafxiia; 28 kda). increased levels of alphafxiia are associated with coronary heart disease, sepsis, and diabetes. biophysical investigation of the structural basis of activation, subst ... | 2003 | 12509996 |
| modifications outside the proteinase binding loop in cucurbita maxima trypsin inhibitor iii (cmti-iii) analogues change the binding energy with bovine beta-trypsin. | five 26-peptide analogues of the trypsin inhibitor [pro18]cmti-iii containing leu or tyr in position 7 and val or tyr in position 27: 1 (leu7, tyr27), 2 (tyr7, val27), 3 (tyr7, tyr27), 4 (leu7, val27) and 5 (leu7, ala18, tyr27) were synthesized by the solid-phase method. analogues 1-4 displayed ka with bovine beta-trypsin of the same order of magnitude as the wild cmti-iii inhibitor, whereas for analogue 5, this value was lower by about 3 orders of magnitude. this indicated that for the analogue ... | 1998 | 9781673 |
| specificity of human cathepsin g. | a series of tetrapeptide p-nitroanilide substrates of the general formula: suc-ala-ala-pro-aaa-p-nitroanilide was used to map the s1 binding pocket of human cathepsin g. based on the kcat/km parameter, the following order of preference was found: lys=phe>arg=leu>met>nle=nva>ala>asp. thus, the enzyme exhibits clear dual and equal trypsin- and chymotrypsin-like specificities. particularly deleterious were beta-branched side chains of ile and val. the p1 substrate preferences found for cathepsin g ... | 1998 | 9675278 |
| the ga2 locus of arabidopsis thaliana encodes ent-kaurene synthase of gibberellin biosynthesis. | the ga2 mutant of arabidopsis thaliana is a gibberellin-deficient dwarf. previous biochemical studies have suggested that the ga2 mutant is impaired in the conversion of copalyl diphosphate to ent-kaurene, which is catalyzed by ent-kaurene synthase (ks). overexpression of the previously isolated ks cdna from pumpkin (cucurbita maxima) (cmks) in the ga2 mutant was able to complement the mutant phenotype. a genomic clone coding for ks, atks, was isolated from a. thaliana using cmks cdna as a heter ... | 1998 | 9536043 |
| gibberellin biosynthesis from gibberellin a12-aldehyde in endosperm and embryos of marah macrocarpus. | soluble enzyme preparations from embryos and endosperm of marah macrocarpus (previously echinocystis macrocarpa) were incubated with [14c4]gibberellin(ga)12-aldehyde, [14c4]ga12, [14c1] ga9, 2,3-didehydro[14c1]ga9, [14c1]ga20, and [17-13c, 3h]ga5. embryo preparations converted ga12-aldehyde, ga12, and ga9 to ga4 and ga7; 2,3-didehydroga9 to ga7; ga5 to ga3; and ga20 (incompletely) to ga1 and ga60, but not to ga3. endosperm preparations converted ga12-aldehyde and ga12 to ga15, ga24, and ga9, but ... | 1997 | 9112781 |
| response of various cucurbits to infection by plasmid-harboring strains of agrobacterium. | tumor formation in cucurbit cultivars resulting from infection by various strains of agrobacterium tumefaciens and agrobacterium rhizogenes is environmentally affected. in all instances, tumors could be induced on excised cotyledons while inoculating attached cotyledons or stems resulted in no tumor formation. in addition, buttercup squash (cucurbita maxima duch. buttercup) was most susceptible to tumor formation, while butterbush squash (cucurbita maxima duch. butterbush) failed to form tumors ... | 1986 | 16665082 |
| conversion of xanthoxin to abscisic acid by cell-free preparations from bean leaves. | cell-free extracts from the leaves of phaseolus vulgaris l. convert xanthoxin to abscisic acid. the enzyme activity in dialyzed or acetone-precipitated extracts shows a strong dependence on either nad or nadp. the enzyme activity appears to be cytosolic with no significant activity observed in chloroplasts. the activity was observed in extracts from roots of phaseolus vulgaris, and also in extracts prepared from the leaves of pisum sativum l., zea mays l., cucurbita maxima duchesne, and vigna ra ... | 1987 | 16665831 |
| properties of a small basic peptide from pumpkin seeds. | a small basic peptide with an unusual amino acid composition has been isolated from the seeds of pumpkin, cucurbita maxima. amino acid analysis and sequence data show the protein to be about 36 residues in length, with an approximate composition lys(1), arg(14), asp(3), (glu + gln)(15), gly(1), pro(1), trp(1). on the basis of composition, the molecular weight is approximately 5000 daltons and the nitrogen content by weight is 20.4%. twelve amino acids are entirely lacking. the peptide is slightl ... | 1988 | 16666381 |
| internal mobility of reactive-site-hydrolyzed recombinant cucurbita maxima trypsin inhibitor-v characterized by nmr spectroscopy: evidence for differential stabilization of newly formed c- and n-termini. | the solution structure and internal dynamics of the reactive-site (lys44-asp45 peptide bond) hydrolyzed form of recombinant cucurbita maxima trypsin inhibitor-v (rcmti-v*) were characterized by the application of two-dimensional 1h-15n nmr methods to the uniformly 15n-labeled protein. the 1h-15n chemical shift correlation spectra of rcmti-v* were assigned, and the chemical shift data were compared with those available for rcmti-v [liu, j., prakash, o., cai, m., gong, y., huang, y., wen, l., wen, ... | 1996 | 8823186 |
| ethnomedicines used in trinidad and tobago for reproductive problems. | throughout history women have tried to control or enhance their fertility using herbal remedies, with various levels of societal support. caribbean folk medicine has been influenced by european folk medicine, either through the early spanish and french settlers or through the continuous immigration of spanish-speaking peoples from venezuela. some folk uses are ancient and were documented by galen and pliny the elder. | 2007 | 17362507 |
| molecular cloning and characterization of a cdna encoding the gibberellin biosynthetic enzyme ent-kaurene synthase b from pumpkin (cucurbita maxima l.). | the first committed step in the formation of diterpenoids leading to gibberellin (ga) biosynthesis is the conversion of geranylgeranyl diphosphate (ggdp) to ent-kaurene. ent-kaurene synthase a (ksa) catalyzes the conversion of ggdp to copalyl diphosphate (cdp), which is subsequently converted to ent-kaurene by ent-kaurene synthase b (ksb). a full-length ksb cdna was isolated from developing cotyledons in immature seeds of pumpkin (cucurbita maxima l.). degenerate oligonucleotide primers were des ... | 1996 | 8771778 |
| solution conformations of proline rings in proteins studied by nmr spectroscopy. | three different conformations of proline rings in a protein in solution, up, down and twist, have been distinguished, and stereospecific assignments of the pyrrolidine beta-, gamma- and delta-hydrogens have been made on the basis of 1h-1h vicinal coupling constant patterns and intraresidue noes. for all three conformations, interhydrogen distances in the pairs alpha-beta 3, beta 3-gamma 3, beta 2-gamma 2, gamma 2-delta 2, and gamma 3-delta 3 (2.3 a) are shorter than those in the pairs alpha-beta ... | 1995 | 8589600 |
| synthesis, cloning and expression in escherichia coli of a gene coding for the met8-->leu cmti i--a representative of the squash inhibitors of serine proteinases. | a chemically synthesized gene coding for a cucurbita maxima trypsin inhibitor modified at position p'3 (met8-->leu cmti i), i.e. at the third position downstream of the reactive site bond (arg5-ile), was cloned into a derivative of the plasmid paed4 that utilizes a t7 expression system. the gene was expressed in escherichia coli as a fusion protein that accumulates in inclusion bodies. after reduction and cnbr cleavage of the fusion protein followed by oxidative refolding and reverse-phase hplc, ... | 1995 | 8543044 |
| single peptide bond hydrolysis/resynthesis in squash inhibitors of serine proteinases. 1. kinetics and thermodynamics of the interaction between squash inhibitors and bovine beta-trypsin. | the substrate and inhibitory parameters are described for the interaction between cucurbita maxima trypsin inhibitor i (cmti i) and bovine beta-trypsin. the data are fully consistent with the reactive site hypothesis and the standard mechanism proposed for the protein inhibitor-serine proteinase interaction. the second-order association rate constant (k(on)) for the interaction of the intact inhibitor and trypsin is high, above 10(6) m-1 s-1. the same value is only 22-fold lower for the reactive ... | 1994 | 8286341 |
| expression cloning of a gibberellin 20-oxidase, a multifunctional enzyme involved in gibberellin biosynthesis. | in the biosynthetic pathway to the gibberellins (gas), carbon-20 is removed by oxidation to give the c19-gas, which include the biologically active plant hormones. we report the isolation of a cdna clone encoding a ga 20-oxidase [gibberellin, 2-oxoglutarate:oxygen oxidoreductase (20-hydroxylating, oxidizing) ec 1.14.11.-] by screening a cdna library from developing cotyledons of pumpkin (cucurbita maxima l.) for expression of this enzyme. when mrna from either the cotyledons or the endosperm was ... | 1994 | 8078921 |
| content and in-vitro accessibility of pro-vitamin a carotenoids from sri lankan cooked non-leafy vegetables and their estimated contribution to vitamin a requirement. | vitamin a deficiency is a public health problem in sri lanka, which affects especially pre-school children. carrots (daucus carota), pumpkins (cucurbita maxima), squashes (cucurbita moschata) and sweet potatoes (ipomoea batatas) of orange, yellow-fleshed varieties are good sources of provitamin a carotenoids, but have not been studied in sri lanka in terms of the food as eaten. the content of carotenoids in each preparation method and the in-vitro accessibility of beta-carotene and alpha-caroten ... | 2007 | 17852483 |
| nutritional and anti-nutritional compositions of cleome rutidosperma, lagenaria siceraria, and cucurbita maxima seeds from nigeria. | flours produced from the seeds of cleome rutidosperma (dc), lagenaria siceraria (mol.), and cucurbita maxima (duch) were evaluated for proximate and elemental compositions. preliminary phytochemical screening was also carried out on the seed flours, which revealed the presence of alkaloids, steroids, pentose, and reducing sugars in all the seed types. tannins, flavonoids, and cardiac glycosides were also detected in only c. rutidosperma seed. the results also showed that the seeds of c. rutidosp ... | 2007 | 18158851 |
| new active analogues of cucurbita maxima trypsin inhibitor iii (cmti-iii) modified in the non-contact region. | four new analogues of trypsin inhibitor cmti-iii(3-28) = [desarg1,desval2,desgly29]cmti-iii which was recently shown to be fully active, were synthesized by the solid-phase method. the introduction of glycine in position 9 (peptide 1) and gly-pro-gly (peptide 2) and gly-pro-asn (peptide 3) in the regions 17-19 and 23-25, respectively, did not change the antitrypsin activity of all modified peptides. all of these substitutions are presumed to be outside the trypsin-binding loop as judged from the ... | 1994 | 8003251 |
| pancreatic proteolytic enzymes of ostrich purified on immobilized protein inhibitors. characterization of a new form of chymotrypsin (chtr1). | four forms of chymotrypsin (chtr1, chtr2, chtr3, chtr4), one form of trypsin and one form of elastase were purified from a slightly alkaline extract of ostrich (struthio camelus) pancreas. the zymogens in the crude extract were activated with immobilized trypsin and then separated by affinity chromatography using immobilized inhibitors and ion exchange chromatography. one of the purified forms of chymotrypsin (chtr1) exhibited an unusual interaction with the highly selective protein trypsin inhi ... | 2008 | 18598777 |
| changing the inhibitory specificity and function of cucurbita maxima trypsin inhibitor-v by site-directed mutagenesis. | cucurbita maxima trypsin inhibitor-v (cmti-v) is also a specific inhibitor of human blood coagulation factor beta-factor xiia. a recombinant version of cmti-v has allowed probing of roles of individual amino acid residues including the reactive site residue, lysine (p1), by site-directed mutagenesis. the k44r showed at least a 5-fold increase in inhibitory activity toward human beta-factor xiia, while there was no change toward bovine trypsin. this result demonstrates that beta-factor-xiia prefe ... | 1995 | 7864895 |
| isolation and expression of three gibberellin 20-oxidase cdna clones from arabidopsis. | using degenerate oligonucleotide primers based on a pumpkin (cucurbita maxima) gibberellin (ga) 20-oxidase sequence, six different fragments of dioxygenase genes were amplified by polymerase chain reaction from arabidopsis thaliana genomic dna. one of these was used to isolate two different full-length cdna clones, at2301 and at2353, from shoots of the ga-deficient arabidopsis mutant ga1-2. a third, related clone, yap169, was identified in the database of expressed sequence tags. the cdna clones ... | 1995 | 7630935 |
| allergy from giant pumpkin (cucurbita maxima) is not a fairy tale. | | 2009 | 19732049 |
| differential responses in yield of pumpkin (cucurbita maxima l.) and nightshade (solanum retroflexum dun.) to the application of three animal manures. | crop responses to different manures differs considerably, however, the factors responsible for it have not been conclusively elucidated. consequently, this study examined the biomass response of cucurbita maxima and solanum retroflexum to application rates of chicken and kraal manures of cattle and goat, and soil factors related to salinity. the crops' biomass yield increased linearly with increase in application rates of kraal and chicken manures, but steeper in the latter. results showed that ... | 2010 | 19954967 |
| the ga5 locus of arabidopsis thaliana encodes a multifunctional gibberellin 20-oxidase: molecular cloning and functional expression. | the biosynthesis of gibberellins (gas) after ga12-aldehyde involves a series of oxidative steps that lead to the formation of bioactive gas. previously, a cdna clone encoding a ga 20-oxidase [gibberellin, 2-oxoglutarate:oxygen oxidoreductase (20-hydroxylating, oxidizing), ec 1.14.11.-] was isolated by immunoscreening a cdna library from liquid endosperm of pumpkin (cucurbita maxima l.) with antibodies against partially purified ga 20-oxidase. here, we report isolation of a genomic clone for ga 2 ... | 1995 | 7604047 |
| reactive-site hydrolyzed cucurbita maxima trypsin inhibitor-v: function, thermodynamic stability, and nmr solution structure. | reactive-site (lys44-asp45 peptide bond) hydrolyzed cucurbita maxima trypsin inhibitor-v (cmti-v*) was prepared and characterized: in comparison to the intact form, cmti-v* exhibited markedly reduced inhibitory properties and binding affinities toward trypsin and human blood coagulation factor xiia. the equilibrium constant of trypsin-catalyzed hydrolysis, khyd, defined as [cmti-v*]/[cmti-v], was measured to be approximately 9.4 at 25 degrees c (delta g degrees = -1.3 kcal.mol-1). from the tempe ... | 1995 | 7547948 |
| agrobacterium-mediated transformation of kabocha squash (cucurbita moschata duch) induced by wounding with aluminum borate whiskers. | an efficient genetic transformation method for kabocha squash (cucurbita moschata duch cv. heiankogiku) was established by wounding cotyledonary node explants with aluminum borate whiskers prior to inoculation with agrobacterium. adventitious shoots were induced from only the proximal regions of the cotyledonary nodes and were most efficiently induced on murashige-skoog agar medium with 1 mg/l benzyladenine. vortexing with 1% (w/v) aluminum borate whiskers significantly increased agrobacterium i ... | 2011 | 21400224 |
| new analogues of cucurbita maxima trypsin inhibitor iii (cmti iii) with simplified structure. | seven new analogues of trypsin inhibitor cmti iii were obtained by solid-phase peptide synthesis. three analogues contained only two, instead of three, disulfide bridges, whereas the molecules of the next four analogues were shortened at the n- and/or c-terminus. the elimination of one disulfide bridge in cmti iii induces a decrease in the association equilibrium constants by 6-7 orders of magnitude, whereas the removal of one, two or three amino-acid residues at the n- and/or c-terminus does no ... | 2006 | 1418676 |
| 1h-n.m.r. studies of squash seed trypsin inhibitor. | 1h-n.m.r. studies at 500 mhz have been performed on a trypsin inhibitor (cmti-iii) found in squash seed (cucurbita maxima). the sequential resonance assignments have been made using two-dimensional techniques. the chemical shifts for the assigned protons are reported at 30 degrees, ph 2.8 and form a basis for the determination of the solution structure of cmti-iii. analysis of the noe data, nh-alpha ch vicinal coupling constants and pattern of slowly exchanging amide protons indicates that the p ... | 2008 | 2613440 |
| looking deep inside: detection of low-abundance proteins in leaf extracts of arabidopsis and phloem exudates of pumpkin. | the field of proteomics suffers from the immense complexity of even small proteomes and the enormous dynamic range of protein concentrations within a given sample. most protein samples contain a few major proteins, which hamper in-depth proteomic analysis. in the human field, combinatorial hexapeptide ligand libraries (cpll; such as proteominer) have been used for reduction of the dynamic range of protein concentrations; however, this technique is not established in plant research. in this work, ... | 2012 | 22555880 |
| pumpkin seed inhibitor of human factor xiia (activated hageman factor) and bovine trypsin. | a strong inhibitor of human hageman factor fragment (hff, beta-factor xiia) and bovine trypsin was isolated from pumpkin (cucurbita maxima) seed extracts by acetone fractionation, by chromatography on columns of diethyl-aminoethylcellulose and carboxylmethyl-sephadex c-25, and by sephadex g-50 gel filtration. pumpkin seed hageman factor inhibitor (phfi) is unusual in its lack of inhibition of several other serine proteinases tested--human plasma, human urinary, and porcine pancreatic kallikreins ... | 1982 | 6215935 |
| screening of trichosanthes kirilowii, momordica charantia and cucurbita maxima (family cucurbitaceae) for compounds with antilipolytic activity. | saline extracts of trichosanthes kirilowii root tubers and momordica charantia seeds were subjected to differential acetone fractionation. the fraction that precipitated after addition of 0.8 vol. acetone was designated api, the fraction that precipitated after addition of another 1.2 vol. acetone was designated apii, and the fraction that precipitated after addition of another volume acetone was designated apiii. api, apii and apiii are fractions enriched with lectins, abortifacient proteins an ... | 1985 | 4058034 |
| sieve element occlusion (seo) genes encode structural phloem proteins involved in wound sealing of the phloem. | the sieve element occlusion (seo) gene family originally was delimited to genes encoding structural components of forisomes, which are specialized crystalloid phloem proteins found solely in the fabaceae. more recently, seo genes discovered in various non-fabaceae plants were proposed to encode the common phloem proteins (p-proteins) that plug sieve plates after wounding. we carried out a comprehensive characterization of two tobacco (nicotiana tabacum) seo genes (ntseo). reporter genes controll ... | 2012 | 22733783 |
| characterization of the pumpkin translationally-controlled tumor protein cmtctp. | in higher plants, the phloem plays a central role in the delivery of nutrients and signals from source to sink tissues. these signals likely coordinate different aspects of plant development, as well as its response to environmental cues. although some phloem-transported proteins and rnas may function as signaling molecules in plants, their mode of action remains poorly understood. previous analysis of transcripts from cmv-infected pumpkin (cucurbita maxima cv big max) identified a translational ... | 2013 | 24065051 |
| overexpression of the pumpkin (cucurbita maxima) 16 kda phloem protein cmpp16 increases tolerance to water deficit. | the phloem plays an important role in the delivery of nutrients and signals between photosynthetic to heterotrophic tissues. proteins and rnas in the phloem translocation stream may have an important role in maintaining the integrity of the sieve tube system, as well as in long-distance signaling. cmpp16 is a pumpkin phloem protein, which has been shown to bind rna in a non-sequence specific manner, and move it cell-to-cell and conceivably, long-distance. the protein and rna are found in both co ... | 2014 | 25482781 |
| fruit quality of seedless watermelon grafted onto squash rootstocks under different production systems. | the market demand for seedless watermelon has been continuously increasing because of consumer preference. grafting is a useful tool to manage soil-borne diseases in watermelon production, but the use of squash rootstocks may negatively affect watermelon fruit quality. currently, most research has focused on seeded cultivars while grafting effects on seedless watermelons remain largely unknown. this multi-season study was conducted to assess the effects of squash rootstocks, including both cucur ... | 2017 | 28369915 |
| [obtention and evaluation of a precooked flour of auyama (cucurbita maxima) and rice, enriched with oleaginous proteins and/or skim milk]. | the purpose of the present study was the production of a precooked blend made of pumpkin squash and rice flour (prb), processed with drum dyer. the blend was supplemented with different protein sources: soy, sesame and deffated milk. the following formulations were obtained: i) prb + 10.5% deffated soy flour; ii) prb + 15% deffated milk; iii) prb + 5% deffated sesame flour + 10% deffated milk; iv) prb + 5% deffated soy flour + 10% deffated sesame flour, and v) prb + 9.5% deffated soy flour + 9.5 ... | 1988 | 3256284 |
| the refined 2.0 a x-ray crystal structure of the complex formed between bovine beta-trypsin and cmti-i, a trypsin inhibitor from squash seeds (cucurbita maxima). topological similarity of the squash seed inhibitors with the carboxypeptidase a inhibitor from potatoes. | the stoichiometric complex formed between bovine beta-trypsin and the cucurbita maxima trypsin inhibitor i (cmti-i) was crystallized and its x-ray crystal structure determined using patterson search techniques. its structure has been crystallographically refined to a final r value of 0.152 (6.0-2.0 a). cmti-i is of ellipsoidal shape; it lacks helices or beta-sheets, but consists of turns and connecting short polypeptide stretches. the disulfide pairing is cys-3i-20i, cys-10i-22i and cys-16i-28i. ... | 1989 | 2914611 |
| isolation and serological comparison of virus-coded proteins of three potyviruses infecting cucurbitaceous plants. | cylindrical inclusion proteins (cips), amorphous inclusion proteins (aips), and virus particles were partially purified from cucurbita maxima leaves infected with zucchini yellow mosaic virus (zymv), watermelon mosaic virus 2 (wmv2), and the watermelon mosaic virus 1 strain of papaya ringspot virus (prsv-w). antisera to these individual antigens, except aips of zymv and wmv2, were prepared and used to determine with electroblot-elisa the serological differentiation indices (sdis) between homolog ... | 1990 | 2338401 |
| synthesis of a wild-type and three mutant cucurbita maxima trypsin inhibitor-encoding genes by a single-strand approach. | a single-strand approach to gene assembly, based on a modification of an in vitro complementary oligodeoxyribonucleotide template-directed ligation of the desired sequence to a linearized vector [chen et al., nucleic acids res. 18 (1990) 871-878], is described. the gene coding for the wild-type cucurbita maxima trypsin inhibitor of 29 amino acid residues [bode et al., febs lett. 242 (1989) 285-292], as well as three mutant forms of the gene, in which two of the three disulfide bonds have been re ... | 1991 | 1937021 |
| buttercup squash provides a marketable alternative to blue hubbard as a trap crop for control of striped cucumber beetles (coleoptera: chrysomelidae). | winter squash is a vital agricultural commodity worldwide. in the northeastern united states, the primary insect pest is the striped cucumber beetle, acalymma vittatum f. using a blue hubbard squash (cucurbita maxima duchesne) perimeter trap crop system can reduce insecticide use by >90% in butternut squash (c. moschata poir), the primary winter squash grown in this region. despite the savings in insecticide costs, growers may be reluctant to give up field space for a perimeter crop of blue hubb ... | 2010 | 22182562 |
| a subclass of plant heat shock cognate 70 chaperones carries a motif that facilitates trafficking through plasmodesmata. | plasmodesmata establish a pathway for the trafficking of non-cell-autonomously acting proteins and ribonucleoprotein complexes. plasmodesmal enriched cell fractions and the contents of enucleate sieve elements, in the form of phloem sap, were used to isolate and characterize heat shock cognate 70 (hsc70) chaperones associated with this cell-to-cell transport pathway. three cucurbita maxima hsc70 chaperones were cloned and functional and sequence analysis led to the identification of a previously ... | 2002 | 12456884 |
| chromogenic substrates of bovine beta-trypsin: the influence of an amino acid residue in p1 position on their interaction with the enzyme. | the cucurbita maxima trypsin inhibitor cmti-iii molecule was used as a vehicle to design and synthesize a series of trypsin chromogenic substrates modified in position p1: ac-ala-val-abu-pro-x-pna, where x = orn, lys, arg, har, arg(no(2)), cit, hci, phe(p-cn), phe(p-nh(2)); pna = p-nitroanilide. the most active compounds (as determined by specificity constant k(cat)/k(m)) were peptides with the arg and lys residues in the position discussed. changes in the length and the decrease of the positive ... | 2001 | 11478806 |
| identification of a volatile attractant for diabrotica and acalymma spp. from blossoms of cucurbita maxima duchesne. | fractionation of headspace volatiles from cucurbita maxima blossoms by high-performance liquid chromatography resulted in the isolation of a single component which was highly active in an electroantennogram bioassay on diabrotica undecimpunctata howardi antennae. this compound was identified as indole by gas chromatography-mass spectrometry. field-trapping bioassays were conducted which indicated that indole is a potent attractant of the western corn rootworm,d. virgifera virgifera, and the stri ... | 1986 | 24306908 |
| design, chemical synthesis and kinetic studies of trypsin chromogenic substrates based on the proteinase binding loop of cucurbita maxima trypsin inhibitor (cmti-iii). | a series of trypsin chromogenic substrates with formula: y-ala-x-abu-pro-lys-pna, where x = gly, ala, abu, val, leu, phe, ser, glu and y = ac, h; pna = p-nitroanilide was synthesized. the cucurbita maxima trypsin inhibitor cmti-iii molecule was used as a vehicle to design the trypsin substrates. to evaluate the influence of position p(4) on the substrate-enzyme interaction, kinetic parameters of newly synthesized substrates with bovine beta-trypsin were determined. the increasing hydrophobicity ... | 2000 | 10694481 |
| the heterogeneity of phloem exudate proteins from different plants: a comparative survey of ten plants using polyacrylamide gel electrophoresis. | proteins in sieve tube exudate from ricinus communis l., acer pseudoplatanus l., aesculus hippocastanum l., cucumis melo l., and two cultivars each of cucumis sativus l., cucurbita pepo l. and cucurbita maxima duchesne were fractionated and compared using polyacrylamide gel electrophoresis. striking differences in major exudate proteins were displayed among the genera and species examined. even cultivars within a single species, although showing general similarities, differed in some prominent p ... | 1976 | 24424985 |
| expression studies of gibberellin oxidases in developing pumpkin seeds. | two cdna clones, 3-ox and 2-ox, have been isolated from developing pumpkin (cucurbita maxima) embryos that show significant amino acid homology to gibberellin (ga) 3-oxidases and 2-oxidases, respectively. recombinant fusion protein of clone 3-ox converted ga(12)-aldehyde, ga(12), ga(15), ga(24), ga(25), and ga(9) to ga(14)-aldehyde, ga(14), ga(37), ga(36), ga(13), and ga(4), respectively. recombinant 2-ox protein oxidized ga(9), ga(4), and ga(1) to ga(51), ga(34), and ga(8), respectively. previo ... | 2003 | 12644672 |
| [preclinical studies of cucurbita maxima (pumpkin seeds) a traditional intestinal antiparasitic in rural urban areas]. | experimental research was carried out at the parasitology and chemistry laboratories of the jorge basadre grohmann national university, in tacna. the process involved two phases: (1) determination of the minimum inhibitory concentration (mic) of cucurbita maxima as an antiparasitic agent using canine tapeworms with an intestinal isolation of 5 to 6 hours, and (2) determination of the side-effects of curbita maxima on exposed albino rats. it was found that the mic of 23 gr. of pumpkin seed in 100 ... | 2004 | 15614300 |
| squash xylem sap has activities that inhibit proliferation and promote the elongation of tobacco by-2 cell protoplasts. | to elucidate the physiological functions of the substances in xylem sap, we analyzed the biological activities of xylem sap from squash (cucurbita maxima duch.) root using tobacco by-2 (nicotiana tabacum l. cv. bright yellow 2) cell protoplasts. when by-2 cell protoplasts were cultivated with the total substance of squash xylem sap, the protoplasts elongated remarkably, and cell division was inhibited. although trans-zeatin riboside (zr), the most abundant cytokinin in squash xylem sap, had a co ... | 2005 | 15890518 |
| purification, characterization, and complete amino acid sequence of a trypsin inhibitor from amaranth (amaranthus hypochondriacus) seeds. | a protein proteinase inhibitor was purified from a seed extract of amaranth (amaranthus hypochondriacus) by precipitation with (nh4)2so4, gel-filtration chromatography, ion-exchange chromatography, and reverse-phase high-performance liquid chromatography. it is a 69-amino acid protein with a high content of valine, arginine, and glutamic acid, but lacking in methionine. the inhibitor has a relative molecular weight of 7400 and an isoelectric point of 7.5. it is a serine proteinase inhibitor that ... | 1993 | 8290633 |
| nitrate reductase from squash: cdna cloning and nitrate regulation. | the assimilation of nitrate in plants involves the reduction of nitrate to ammonia in two steps. the first step requires nitrate reductase, a nitrate-inducible enzyme. when seedlings of squash (cucurbita maxima l.) were treated with nitrate, both nitrate reductase activity and protein were induced in the cotyledons. poly(a)(+) rna was prepared from cotyledons of nitrate-treated seedlings and was used to construct a lambdagt11 cdna library. using antibodies from mice immunized against purified ni ... | 1986 | 16593773 |
| indigenous and traditional plants: south african parents' knowledge, perceptions and uses and their children's sensory acceptance. | the dietary shift from indigenous and traditional plants (itps) to cash crops and exotic plant food sources increases the risk of malnutrition and other nutrition-related non-communicable diseases, especially in poor rural communities. farm communities in south africa have been associated with poor nutritional status and extreme poverty. itps have been found to be affordable sources of several micronutrients. however, knowledge of and the use of these plants are declining, and little is known ab ... | 2013 | 24274394 |
| herbivore-triggered electrophysiological reactions: candidates for systemic signals in higher plants and the challenge of their identification. | in stressed plants, electrophysiological reactions (elrs) are presumed to contribute to long-distance intercellular communication between distant plant parts. because of the focus on abiotic stress-induced elrs in recent decades, biotic stress-triggered elrs have been widely ignored. it is likely that the challenge to identify the particular elr types (action potential [ap], variation potential, and system potential [sp]) was responsible for this course of action. thus, this survey focused on in ... | 2016 | 26872949 |
| a putative acyl-coa-binding protein is a major phloem sap protein in rice (oryza sativa l.). | the n-terminal amino-acid sequence of a major rice phloem-sap protein, named rpp10, was determined. rpp10 is encoded by a single gene in the rice genome. its complete amino-acid sequence, predicted from the corresponding rice full-length cdna, showed high similarity to plant acyl-coa-binding proteins (acbps). western blot analysis using anti-acbp antiserum revealed that putative acbp is abundant in the phloem sap of rice plants, and is also present in sieve-tube exudates of winter squash (cucurb ... | 2006 | 16804052 |
| identification of a dispersed mboi repeat family in five higher plant genomes. | digestion of nuclear dnas of five plants, namely cucurbita maxima (red gourd), trichosanthes anguina (snake gourd), cucumis sativus (cucumber), cajanus cajan (pigeon pea) and phaseolus vulgaris (french bean) with the restriction endonuclease mboi yielded discrete size classes with molecular weights in the range of 0.5 to 5 kbp. the mboi digestion pattern of cot 0.1 dna in french bean is comparable with that of total dna, indicating that these bands represented highly repeated dna sequences. clea ... | 1988 | 3233342 |
| dietary patterns and risk of gastric cancer: a case-control study in uruguay. | gastric cancer is a frequent malignancy in the uruguayan population. in northern counties, incidence rates reach high figures (age-standardized rates [asr], 37.3 per 100,000 men and 18.3 per 100,000 women). diet is a major determinant in gastric carcinogenesis. because foods or food groups have the advantage over nutrients in being most directly related to dietary recommendations, we decided to conduct a case-control study on the relationships between food groups and risk of gastric cancer. for ... | 2004 | 15616769 |
| molecular and biochemical characterization of the capsicum annuum calcium-dependent protein kinase 3 (cacdpk3) gene induced by abiotic and biotic stresses. | the isolated full-length capsicum annuum calcium-dependent protein kinase 3 (cacdpk3) cdna clone was selected from the chili pepper expressed sequence tag database (http://www.pdrc.re.kr/ks200201/pepper.html). phylogenetic analysis based on the deduced amino acid sequence of cacdpk3 cdna revealed significant sequence similarity to the winter squash (cucurbita maxima) cmcpk2 gene (81% identity). genomic gel blot analysis disclosed that cacdpk3 belongs to a multigene family in the pepper genome. c ... | 2004 | 15449060 |
| oil and tocopherol content and composition of pumpkin seed oil in 12 cultivars. | twelve pumpkin cultivars (cucurbita maxima d.), cultivated in iowa, were studied for their seed oil content, fatty acid composition, and tocopherol content. oil content ranged from 10.9 to 30.9%. total unsaturated fatty acid content ranged from 73.1 to 80.5%. the predominant fatty acids present were linoleic, oleic, palmitic, and stearic. significant differences were observed among the cultivars for stearic, oleic, linoleic, and gadoleic acid content of oil. low linolenic acid levels were observ ... | 2007 | 17439238 |
| flowering locus t protein may act as the long-distance florigenic signal in the cucurbits. | cucurbita moschata, a cucurbit species responsive to inductive short-day (sd) photoperiods, and zucchini yellow mosaic virus (zymv) were used to test whether long-distance movement of flowering locus t (ft) mrna or ft is required for floral induction. ectopic expression of ft by zymv was highly effective in mediating floral induction of long-day (ld)-treated plants. moreover, the infection zone of zymv was far removed from floral meristems, suggesting that ft transcripts do not function as the f ... | 2007 | 17540715 |
| reciprocal phosphorylation and glycosylation recognition motifs control ncapp1 interaction with pumpkin phloem proteins and their cell-to-cell movement. | in plants, cell-to-cell trafficking of non-cell-autonomous proteins (ncaps) involves protein-protein interactions, and a role for posttranslational modification has been implicated. in this study, proteins contained in pumpkin (cucurbita maxima cv big max) phloem sap were used as a source of ncaps to further explore the molecular basis for selective ncap trafficking. protein overlay assays and coimmunoprecipitation experiments established that phosphorylation and glycosylation, on both nicotiana ... | 2007 | 17601822 |
| isolation and expression of ga 2-oxidase2 in tomato. | ga 2-oxidases, a key enzyme involves ga biosynthesis, catalyze the degradation of active c(19)-gibberellins (gas) through 2-hydroxylation yields inactive ga product. searching public tomato database, the putative ga2ox2 sequences were assembled. we isolated a full-length ga2ox2 cdna with primers designed from the assembled sequence. this gene was designed as slga2ox2 (genbank accession no. ef017805). the full-length ga2ox2 gene contained a complete open reading frame (orf) of 1203 bp, which enco ... | 2007 | 17676476 |
| engineered resistance against proteinases. | exogenous proteinase inhibitors are valuable and economically interesting protective biotechnological tools. we examined whether small proteinase inhibitors when fused to a selected target protein can protect the target from proteolytic degradation without simultaneously affecting the function and activity of the target domain. two proteinase inhibitors were studied: a kazal-type silk proteinase inhibitor (spi2) from galleria mellonella, and the cucurbita maxima trypsin inhibitor i (cmti i). bot ... | 2007 | 17823663 |
| uptake, translocation, and accumulation of manufactured iron oxide nanoparticles by pumpkin plants. | rapid development and application of nanomaterials and nanotechnology make assessment of their potential health and environmental impacts on humans, non-human biota, and ecosystems imperative. here we show that pumpkin plants (cucurbita maxima), grown in an aqueous medium containing magnetite (fe3o4) nanoparticles, can absorb, translocate, and accumulate the particles in the plant tissues. these results suggest that plants, as an important component of the environmental and ecological systems, n ... | 2008 | 18528537 |
| a comparative analysis of phloem exudate proteins from cucumis melo, cucumis sativus and cucurbita maxima by polyacrylamide gel electrophoresis and isoelectric focusing. | proteins in sieve tube exudate from cucumis melo l., cucumis sativus l. and cucurbita maxima duch. were analysed by gel electrophoresis and isoelectric focusing. estimated molecular weights and isoelectric points for the major and minor proteins from each plant species are presented. electrophoresis revealed striking differences between the protein complements of exudatc from the two genera investigated. similarly, although a few exudate proteins from the two species of cucumis possessed identic ... | 1976 | 24424601 |
| can adverse effects of acidity and aluminum toxicity be alleviated by appropriate rootstock selection in cucumber? | low-ph and aluminum (al) stresses are the major constraints that limit crop yield in acidic soils. grafting vegetable elite cultivars onto appropriate rootstocks may represent an effective tool to improve crop tolerance to acidity and al toxicity. two greenhouse hydroponic experiments were performed to evaluate growth, yield, biomass production, chlorophyll index, electrolyte leakage, mineral composition, and assimilate partitioning in plant tissues of cucumber plants (cucumis sativus l. "ekron" ... | 2016 | 27621740 |
| melon resistance to cucurbit yellow stunting disorder virus is characterized by reduced virus accumulation. | abstract the pattern of accumulation of cucurbit yellow stunting disorder virus (cysdv; genus crinivirus, family closteroviridae) rna has been analyzed in several cucurbit accessions. in susceptible accessions of melon (cucumis melo), cucumber (cucumis sativus), marrow (cucurbita maxima), and squash (cucurbita pepo), cysdv rna accumulation peaked during the first to second week postinoculation in the first to third leaf above the inoculated one; younger leaves showed very low or undetectable lev ... | 2003 | 18943165 |
| using trap crops for control of acalymma vittatum (coleoptera: chrysomelidae) reduces insecticide use in butternut squash. | striped cucumber beetle, acalymma vittatum f., is the primary insect pest of cucurbit crops in the northeastern united states. adult beetles colonize squash crops from field borders, causing feeding damage at the seedling stage and transmitting bacterial wilt erwinia tracheiphila hauben et al. 1999. conventional control methods rely on insecticide applications to the entire field, but surrounding main crops with a more attractive perimeter could reduce reliance on insecticides. a. cittatum shows ... | 2009 | 19610425 |
| assessing risk to human health from tropical leafy vegetables grown on contaminated urban soils. | fifteen tropical leafy vegetable types were sampled from farmers' gardens situated on nine contaminated sites used to grow vegetables for commercial or subsistence consumption in and around kampala city, uganda. trace metal concentrations in soils were highly variable and originated from irrigation with wastewater, effluent discharge from industry and dumping of solid waste. metal concentrations in the edible shoots of vegetables also differed greatly between, and within, sites. gynandropsis gyn ... | 2010 | 20739044 |
| pumpkin eif5a isoforms interact with components of the translational machinery in the cucurbit sieve tube system. | in yeast, eif5a, in combination with eef2, functions at the translation step, during the protein elongation cycle. this result is of significance with respect to functioning of the enucleate sieve tube system, as eif5a was recently detected in cucurbita maxima (pumpkin) phloem sap. in the present study, we further characterized four cmeif5a isoforms, encoding three proteins, all of which were present in the phloem sap. although hypusination of cmeif5a was not necessary for entry into the sieve e ... | 2010 | 20807213 |
| structural divergence of plant tctps. | the translationally controlled tumor protein (tctp) is a highly conserved protein at the level of sequence, considered to play an essential role in the regulation of growth and development in eukaryotes. however, this function has been inferred from studies in a few model systems, such as mice and mammalian cell lines, drosophila and arabidopsis. thus, the knowledge regarding this protein is far from complete. in the present study bioinformatic analysis showed the presence of one or more tctp ge ... | 2014 | 25120549 |
| ectopic expression of pumpkin gibberellin oxidases alters gibberellin biosynthesis and development of transgenic arabidopsis plants. | immature pumpkin (cucurbita maxima) seeds contain gibberellin (ga) oxidases with unique catalytic properties resulting in gas of unknown function for plant growth and development. overexpression of pumpkin ga 7-oxidase (cmga7ox) in arabidopsis (arabidopsis thaliana) resulted in seedlings with elongated roots, taller plants that flower earlier with only a little increase in bioactive ga4 levels compared to control plants. in the same way, overexpression of the pumpkin ga 3-oxidase1 (cmga3ox1) res ... | 2006 | 16384902 |
| relationship between crop losses and initial population densities of meloidogyne arenaria in winter-grown oriental melon in korea. | to determine the economic threshold level, oriental melon (cucumis melo l. cv. geumssaragi-euncheon) grafted on shintozoa (cucurbita maxima x cu. moschata) was planted in plots (2 x 3 m) under a plastic film in february with a range of initial population densities (pi) of meloidogyne arenaria. the relationships of early, late, and total yield to pi measured in september and january were adequately described by both linear regression and the seinhorst damage model. initial nematode densities in s ... | 2002 | 19265907 |
| microplot evaluation of rootstocks for control of meloidogyne incognita on grafted tomato, muskmelon, and watermelon. | microplot experiments were conducted over two years (four growing seasons) to evaluate meloidogyne incognita resistance in rootstocks used for grafted tomato (solanum lycopersicum), muskmelon (cucumis melo), and watermelon (citrullus lanatus). three tomato rootstocks; 'tx301', 'multifort', and 'aloha', were tested in addition to the nongrafted scion, 'florida-47'. two muskmelon rootstocks; cucumis metuliferus and 'tetsukabuto' (cucurbita maxima × cucurbita moschata) were evaluated with the nongr ... | 2011 | 23431109 |
| proteomics of curcurbit phloem exudate reveals a network of defence proteins. | many different proteins can be separated from the sap of mature sieve tubes of different plant species. to date, only a limited number of those have been identified and functionally characterised. due to sieve tubes inability of transcription and translation, the proteins are most probably synthesised in the intimately connected companion cells and transported into the sieve elements through plasmodesmata. the specific protein composition of phloem sap suggests an important role of these protein ... | 2004 | 15276438 |
| a systemic small rna signaling system in plants. | systemic translocation of rna exerts non-cell-autonomous control over plant development and defense. long-distance delivery of mrna has been proven, but transport of small interfering rna and microrna remains to be demonstrated. analyses performed on phloem sap collected from a range of plants identified populations of small rna species. the dynamic nature of this population was reflected in its response to growth conditions and viral infection. the authenticity of these phloem small rna molecul ... | 2004 | 15258266 |
| yield response of pumpkin and winter squash to simulated cucumber beetle (coleoptera: chrysomelidae) feeding injury. | field studies were conducted to characterize the yield responses of pumpkin, cucurbita pepo l., and wintersquash, cucurbita maxima duch., to simulated striped cucumber beetle, acalymma vittatum (f.), feeding damage. preliminary studies in pumpkin ('spookie') were conducted in 1993, and more complete studies in 1994 and 1995 in pumpkin (spookie), and in 1995 and 1996 in winter squash ('waltham'). plants were artificially injured 1 time at the cotyledon, 1st, 2nd, or 3rd true leaf stages by clippi ... | 2000 | 14658523 |
| aphid salivary proteases are capable of degrading sieve-tube proteins. | sieve tubes serve as transport conduits for photo-assimilates and other resources in angiosperms and are profitable targets for piercing-sucking insects such as aphids. sieve-tube sap also contains significant amounts of proteins with diverse functions, for example in signalling, metabolism, and defence. the identification of salivary proteases in acyrthosiphon pisum led to the hypothesis that aphids might be able to digest these proteins and by doing so suppress plant defence and access additio ... | 2015 | 25540441 |
| effects of 60-hz electric fields on cellular elongation and radial expansion growth in cucurbit roots. | serial longitudinal and transverse sections were prepared from roots of cucumis sativus and cucurbita maxima that had been exposed/sham-exposed to 60-hz electric fields for 0-2 days. field exposures were selected to produce a 10-20% or a 70-80% growth inhibition in whole roots of both species. cortical cell length and diameter were measured using a microscope and eyepiece micrometer; measurements were conducted "blind." in both species, inhibition of cellular elongation was associated with expos ... | 1987 | 3580000 |
| bioactivities of some essential oils against the camel nasal botfly, cephalopina titillator. | nasopharyngeal myiasis of camels is caused by the larvae of cephalopina titillator. we determined the efficacy of essential oils (eos) of pumpkin, cucurbita maxima; lupinus, lupinus luteus; garlic oil, allium sativum; and peppermint, mentha piperita, against the third larval stage of c. titillator using larval immersion tests. the positive control group was treated with ivermectin and the negative control one was treated with distilled water and few drops of tween 80. larvae were reared until ad ... | 2014 | 24276644 |
| electrophysiology of pumpkin seeds: memristors in vivo. | leon chua, the discoverer of a memristor, theoretically predicted that voltage gated ion channels can be memristors. we recently found memristors in different plants such as the venus flytrap, mimosa pudica, aloe vera, apple fruits, and in potato tubers. there are no publications in literature about the existence of memristors in seeds. the goal of this work was to discover if pumpkin seeds might have memristors. we selected cucurbita pepo l., cv. cinderella, cucurbita maxima l. cv warty goblin, ... | 2016 | 26926652 |
| induction of elf transmembrane potentials in relation to power-frequency electric field bioeffects in a plant root model system. i. relationship between applied field strength and cucurbitaceous root growth rates. | seminal roots of cucumis sativus and cucurbita maxima were exposed to 60 hz electric fields of 100-500 v x m-1 in a conducting aqueous inorganic growth medium. root growth rates were measured to produce a dose-response relationship for each species. the species were selected for study because of their familial relationship, reported sensitivity to 60 hz, 360 v x m-1 electric fields, and differing average root cell sizes. the latter characteristic influences the magnitude of elf membrane potentia ... | 1986 | 3763827 |