| effect of acid and salt concentration in fresh-pack pickles on the growth of clostridium botulinum spores. | the addition of various amounts of acetic acid to pureed cucumbers inoculated with clostridium botulinum spores has shown that outgrowth is inhibited at ph 4.8 but not at ph 5.0. inoculation experiments with whole cucumbers showed that as little as 0.9% acetic acid in the brine was sufficient to prevent outgrowth from spore inocula as high as 10(6)/cucumber. it was further shown that the rapid rate of acetic acid penetration into fresh-pack pickles prevents the growth of any c. botulinum spores ... | 1976 | 9898 |
| influence of nitrogen supply on host and multiplication of cucumber mosaic virus. | the effect of several concentrations of nitrogen (0, 21, 70, 210, 420 and 630 ppm) on the growth and multiplication of cucumber mosaic virus in cucumber (cucumis sativus l. barshati) grown in sand culture was studied. the effect of nitrogen nutrition on the concentration of virus appeared to be directly related to vegetative growth of the host. 420 ppm level yielding the best growth also showed the highest virus concentration and total nitrogen percentage at 30th day of infection. | 1977 | 67769 |
| plant microbody proteins. purification and glycoprotein nature of glyoxysomal isocitrate lyase from cucumber cotyledons. | 1. isocitrate lyase from cotyledons of cucumber seedlings (cucumis sativus) has been purified 100-fold. two methods of preparing the soluble glyoxylate cycle enzyme are described: an elaborated method which used crude extracts of cucumber cotyledons, and another procedure which started with purified glyoxysomes from 4-day-old cotyledons and included a separation of glyoxysomal matrix enzymes by zonal centrifugation. the product behaved as a single species when tested by (a) polyacrylamide gel el ... | 1978 | 103713 |
| occurrence of patulin in fruit and vegetables. | in different varieties of apples and pears suffering from brown rot, patulin was found in about 50 p. 100 of samples investigated (about 120). patulin levels as high as 1 g/kg rotten material were found 2-3 days after the fruit was removed from the cold store where it had been stored for 5 months. a significant diffusion into the healthy plant tissue was not observed in apples, but in peaches, tomatoes and pears. vegetable products with natural patulin content: apples, pears, peaches, apricots, ... | 1977 | 613910 |
| [mode of action and inhibition of polygalacturonase covalently bound to polysaccharide and glass carriers]. | endo-polygalacturonase (ec 3.2.1.15.) from aspergillus spec. is much changed as far as its mode of action and the interaction with vegetable inhibitors of pectinase (from green beans and cucumbers) are concerned when it is covalently bound to insoluble carriers (sepharose, cellulose powder, macroporous glass and nonporous ballotinis). whereas a 2% degradation of substrate by the soluble enzyme caused a 50% decrease of viscosity of citrus pectic acid, the comparable degradation of substrate was i ... | 1978 | 692641 |
| [patulin in foods of vegetable origin. 2. several kinds of fruit and vegetables and fruit and vegetable products (author's transl)]. | patulin was found in fruit with spontaneous brown rot (bananas, pineapples, grapes, peaches, apricots) as well as in moldy compots and in sallow-thorn juice. fruit, vegetables and fruit and vegetable product were artificially infected with penicillium expansum, p. urticae and byssochlamys nivea; patulin was subsequently found in peaches, apricots, greengages, bananas, strawberries, honeydew melons, tomatoes, red and green paprika, cucumbers and carrots; in several kinds of compot, in tomato juic ... | 1977 | 857490 |
| effects of tentoxin on enzymic activities in cucumber and cabbage cotyledons. | tentoxin, produced by alternaria alternata (fr.) keissl. causes severe variegated chlorosis in germinating seedlings of certain dicotyledonous species. however, it does not impair radicle and hypocotyl elongation or cotyledon expansion. effects of the toxin on the activity of selected enzymes from both chloroplasts and cytoplasm were determined. cucumber (cucumis sativus l.), which is highly sensitive to tentoxin and cabbage (brassica oleracea l.), which is resistant, were used as test plants. t ... | 1975 | 1171367 |
| viability of vibrio cholerae biotype el tor and of cholera phage on vegetables. | different vegetables were contaminated with sewage water containing large amounts of el tor vibrios, and the viability of these vibrios was studied under defined conditions of storage. during the dry season (summer) in jerusalem, the el tor vibrios survived for up to 24 hr on parsley, 24 to 30 hr on tomatoes and carrots, 24 to 48 hr on cucumbers, peppers and okra and two to three days on lettuce, when the vegetables were stored at room temperature. the survival time was longer during the rainy s ... | 1975 | 1184361 |
| stearoyl-acyl-carrier-protein desaturase from higher plants is structurally unrelated to the animal and fungal homologs. | stearoyl-acyl-carrier-protein (acp) desaturase (ec 1.14.99.6) was purified to homogeneity from avocado mesocarp, and monospecific polyclonal antibodies directed against the protein were used to isolate full-length cdna clones from ricinus communis (castor) seed and cucumis sativus (cucumber). the nucleotide sequence of the castor clone prcd1 revealed an open reading frame of 1.2 kilobases encoding a 396-amino acid protein of 45 kda. the cucumber clone pcsd1 encoded a homologous 396-amino acid pr ... | 1991 | 2006187 |
| molecular cloning of the gene for indolepyruvate decarboxylase from enterobacter cloacae. | although indole-3-acetic acid (iaa) is a well-known plant hormone, the main iaa biosynthetic pathway from l-tryptophan (trp) via indole-3-pyruvic acid (ipya) has yet to be elucidated. previous studies have suggested that iaa is produced by enterobacter cloacae isolated from the rhizosphere of cucumbers and its biosynthetic pathway may possibly be the same as that in plants. to elucidate this pathway, the iaa biosynthetic gene was isolated from a genomic library of e. cloacae by assaying for the ... | 1991 | 2034209 |
| isolation and sequencing of cdna clones encoding ethylene-induced putative peroxidases from cucumber cotyledons. | a cdna library from ethephon-treated cucumber cotyledons (cucumis sativus l. cv. poinsett 76) was constructed. two cdna clones encoding putative peroxidases were isolated by means of a synthetic probe based on a partial amino acid sequence of a 33 kda cationic peroxidase that had been previously shown to be induced by ethylene. dna sequencing indicates that the two clones were derived from two closely related rna species that are related to published plant peroxidase sequences. southern analysis ... | 1990 | 2102850 |
| length heterogeneity of the rrna precursor in cucumber (cucumis sativus). | the length homogeneous part of the intergenic spacer (igs) of the 18s-25s ribosomal rna genes of cucumber (cucumis sativus) was characterized by sequencing 2389 bp preceding the 18s rrna coding region of a 12.5 kbp repeat type. this part of the igs is composed of repeated elements and shows a very complex structural organization. most obvious is a 119 bp element which is repeated seven times. a single transcription initiation site (tis) was detected by a 't4 polymerase stop' experiment upstream ... | 1990 | 2103464 |
| gc balance in the internal transcribed spacers its 1 and its 2 of nuclear ribosomal rna genes. | the internal transcribed spacer (its) 1 and 2, the 5.8s rrna gene, and adjacent 18s rrna and 25s rrna coding regions of two cucurbitaceae (cucurbita pepo, zucchini, its 1: 187 bp, and its 2: 252 bp in length, and cucumis sativus, cucumber, its 1: 229 bp, and its 2: 245 bp in length) have been sequenced. the evolutionary pattern shown by the itss of these plants is different from that found in vertebrates. deletions, insertions, and base substitutions have occurred in both spacers; however, it is ... | 1990 | 2107331 |
| cloning and sequencing of the malate synthase gene from hansenula polymorpha. | we have cloned the mas gene, encoding the microbody matrix enzyme malate synthase (ec 4.1.3.2.) from the methylotrophic yeast hansenula polymorpha. the gene was isolated by screening of a genomic library with a mixed-sequence probe, based on the partial amino acid sequence of the purified enzyme. the nucleotide sequence of a 2.4-kilobase stretch of dna covering the mas gene was determined. the gene contains an open reading frame of 555 amino acids, amounting to a calculated molecular mass of 63, ... | 1990 | 2349836 |
| the malate synthase gene of cucumber. | the complete sequences of a full-length cdna clone and a genomic clone encoding the cucumis sativus glyoxysomal enzyme malate synthase, have been determined. the sequences have enabled us to identify putative control regions at the 5' end of the gene, three introns, and possible alternative polyadenylation sites at the 3' end. the deduced amino acid sequence predicts a polypeptide of 64,961 molecular weight, which has 48% identity with that of escherichia coli. comparison of the sequence of mala ... | 1989 | 2491683 |
| listeria spp. found on fresh market produce. | from october 1987 to august 1988, 1,000 tests were conducted on 10 types of fresh produce from two minneapolis area supermarkets to detect listeria spp. the produce included broccoli, cabbage, carrots, cauliflower, cucumbers, lettuce, mushrooms, potatoes, radishes, and tomatoes. the vegetables were tested by the food and drug administration method for isolation of listeria spp., with the addition of licl-phenylethanol-moxalactam agar in the last 280 tests; 8.6 and 11.4% of these tests were posit ... | 1989 | 2506809 |
| coat protein of melon necrotic spot carmovirus is more similar to those of tombusviruses than those of carmoviruses. | complementary dna copies of the genomic rna of melon necrotic spot virus (mnsv) have been cloned and the region deduced to encode the coat protein has been sequenced. the putative coat protein coding region, located near the 3' end of the genome, consists of 1170 nucleotides and has the potential to encode a 390 amino acid protein of mr 41,840. our data show that although mnsv is a carmovirus, its coat protein more closely resembles those of the tombusviruses than those of the carmoviruses seque ... | 1989 | 2584953 |
| blood sugar lowering potentiality of selected cucurbitaceae plants of indian origin. | using five experimental models, the blood sugar lowering efficacy of eight plants of cucurbitaceae family has been assessed. the ethanolic extract of cucumis sativus linn, cucumis melo utilissimum roxb, cucumis melo linn, benincasa hispida thunb cogn and tricosanthes anguina nees, when administered in 250 mg/kg dose, orally to rats failed to lower blood sugar or to depress the peak value, after glucose load. however, ethanolic extract of momordica charantia linn plant and coccinia indica whit an ... | 1989 | 2620957 |
| primary structure of cucumber (cucumis sativus) ascorbate oxidase deduced from cdna sequence: homology with blue copper proteins and tissue-specific expression. | cdna clones for ascorbate oxidase were isolated from a cdna library made from cucumber (cucumis sativus) fruit mrna. the library was screened with synthetic oligonucleotides that encode the nh2-terminal sequence of this enzyme. nucleotide sequence analysis of the cloned cdna inserts revealed a 1761-base-pair open reading frame that encoded an nh2-terminal signal peptide of 33 amino acids and a mature enzyme of 554 amino acids (mr, 62,258). the amino acid sequence deduced from nucleotide sequence ... | 1989 | 2919172 |
| phase determination by multiple-wavelength x-ray diffraction: crystal structure of a basic "blue" copper protein from cucumbers. | a novel x-ray diffraction technique, multiple-wavelength anomalous dispersion (mad) phasing, has been applied to the de novo determination of an unknown protein structure, that of the "blue" copper protein isolated from cucumber seedlings. this method makes use of crystallographic phases determined from measurements made at several wavelengths and has recently been made technically feasible through the use of intense, polychromatic synchrotron radiation together with accurate data collection fro ... | 1988 | 3406739 |
| rna-directed rna polymerases from healthy and from virus-infected cucumber. | much work has been done on the isolation, purification, and characterization of the rna-directed rna polymerase (ec 2.7.7.48) of cucumber mosaic virus (cmv)-infected cucumbers. uninfected plants were reported to have no such enzyme, but we recently detected low levels of the activity in cucumber. since tobacco and cowpea contain such an enzyme that is variably increased in amount by various virus (as well as viroid) infections, we assumed that this would also be the case upon cmv infection of cu ... | 1986 | 3458203 |
| [biological value of hydroponically grown cucumbers and tomatoes]. | | 1969 | 4312646 |
| introduction of pseudomonas aeruginosa into a hospital via vegetables. | pseudomonas aeruginosa was isolated from tomatoes, radishes, celery, carrots, endive, cabbage, cucumbers, onions, and lettuce obtained from the kitchen of a general hospital, with tomatoes yielding both highest frequencies of isolation and highest counts. presence of p. aeruginosa on the hands of kitchen personnel and cutting boards and knives which they used suggests acquisition of the organism through contact with these vegetables. it is estimated that a patient consuming an average portion of ... | 1972 | 4628795 |
| [soft rot and wilt of cucumbers caused by pseudomonas burgeri (pot.)]. | | 1972 | 4663475 |
| the effects of tentoxin on chlorophyll synthesis and plastid structure in cucumber and cabbage. | to determine if chlorosis caused by tentoxin, a toxin produced by alternaria tenuis nees., is due to interference with chlorophyll synthesis directly or to disruption of normal chloroplast development, the effects of the toxin on these processes in cucumber (cucumis sativus l.) and cabbage (brassica oleracea l., var. capitata) were studied. cucumber cotyledons are highly sensitive to the toxin but exhibited no interference with the conversion of protochlorophyll(ide) to chlorophyll(ide) or with ... | 1970 | 5463696 |
| properties of a plant virus-induced rna polymerase in particulate fractions of cucumbers infected with cucumber mosaic virus. | | 1970 | 5474461 |
| properties of a plant virus-induced rna polymerase in cucumbers infected with cucumber mosaic virus. | | 1968 | 5684122 |
| histological effect of certain pickles on the human gastric mucosa. a preliminary report. | the histological effects of several types of pickles and control substances were assessed by repeated suction (per os) biopsies of the gastric antrum in three healthy men. the ingestion of 30 ounces of fukujinzuke (assorted vegetables pickled in soy sauce) or of vinegared gherkins over a three-day period caused marked changes in the surface epithelium and gastric pits in all three subjects. the abnormalities consisted of loss of cellular mucus, nuclear enlargement, prominent nuclear chromatin an ... | 1967 | 6026337 |
| [isolation of the virus of tobacco necrosis from the fruits of field cucumbers]. | | 1967 | 6082776 |
| a comparative study in vitro of physiological activity of triterpene glycosides of marine invertebrates of echinoderm type. | 1. the cytostatic and antimicrobial activity of triterpene glycosides of 19 holothurian species of the pacific tropical zone has been studied. 2. it has been demonstrated that yeast and tumor cells display a comparable sensibility to the action of triterpene oligosides of sea cucumbers. 3. gram-positive and gram-negative bacteria are not sensible to the action of glycosides in doses to 500 mkg/ml. 4. triterpene glycosides-stichoposides, thelothurins and oligosides of holothuria of genus bohadsch ... | 1982 | 6128178 |
| effect of marine glycosides on adenosinetriphosphatase activity. | marine glycosides from the sea cucumbers actinopyga agassizi, holothuria atra, bohadschia argus, cucumaria fraudatrix, astichopus multifidus and thelenota ananas inhibit both na+-k+ atpase and mg2+-atpase of rat brain in vitro. the glycoside-cholesterol complex of these compounds does not influence atpase activity. asterosaponins from starfishes linckia guildingi and linckia laevigata possess a slight inhibiting effect. the triterpene glycosides from sea cucumbers are more powerful inhibitors th ... | 1982 | 6213068 |
| aspartyl proteinase from cucumber (cucumis sativus) seeds. preparation and characteristics. | aspartyl proteinase (ec 3.4.23) from cucumber seeds was purified by ammonium sulphate fractionation, chromatography on immobilized pepstatin and gel filtration on sephacryl s-200. the preparation obtained, homogeneous on polyacrylamide-gel electrophoresis in acidic and alkaline media, has a molecular mass of 42,000, pi of 5.2, and shows the highest activity with denatured haemoglobin at ph 3.2. the proteinase is stable in slightly alkaline medium, whereas it is inactivated in acidic medium, espe ... | 1983 | 6346762 |
| [capacity of triterpene glycosides from holothurians to stimulate antibacterial resistance in a model of experimental murine salmonellosis]. | when injected intraperitoneally into mice in doses of 40.0-0.4 microgram, cucumarioside, the preparation of triterpene glycosides obtained from sea cucumbers (cusumaria japonica), enhanced the resistance of the animals to the subsequent challenge with salmonella typhimurium. the study of the duration of the persistence of salmonellae in mice receiving the preparation in a dose of 0.001 microgram revealed a decrease in the contamination of their organs. the same dose of the preparation stimulated ... | 1984 | 6377780 |
| [effect of cucumarioside (a triterpene glycoside from the holothurian cucumaria japonica) on the development of an immune response in mice to corpuscular pertussis vaccine]. | the influence of cucumarioside, triterpene glycoside obtained from cucumaria japonica (echinodermata, holoturioidea), or sea cucumbers, on the resistance of mice to bordetella pertussis infection (with the use experimental pertussis meningoencephalitis as a model) and on the development of immune response to corpuscular pertussis vaccine was studied. the preparation under test was shown to have greatly pronounced immunomodulating properties depending on both the concentration of cucumarioside an ... | 1984 | 6506936 |
| survey of fresh vegetables for nematodes, amoebae, and salmonella. | contamination by nematodes, amoebae, and bacteria of the genus salmonella was estimated in a 2-year survey of salad vegetables obtained from wholesale and retail sources. the vegetables examined were cucumbers, cabbage, lettuce, celery, carrots, radishes, tomatoes, mushrooms, cauliflower, and spinach. nematode eggs and larvae were recovered by the nacconol-ether centrifugation method. some nematode eggs were identified as parasitic ascaris sp.; the majority of larval nematodes were thought to be ... | 1984 | 6540260 |
| fluorescent pseudomonads in the rhizosphere of plants and their relation to root exudates. | fluorescent pseudomonads were present in chernozem soil not influenced by plant roots (10(3)-10(4) per g dry soil) in the rhizosphere soil of various plants (10(4)-10(5) per g soil) and on roots (10(3) to 10(7) per g fresh roots), depending on the species and age of the plant. relative species representation of fluorescent pseudomonads changed on the roots and in the plant rhizosphere as compared with free soil. pseudomonas fluorescens, representing 60-93% of the population of fluorescent pseudo ... | 1980 | 6769769 |
| carcinogenicity of dichlorvos. | dichlorvos, 2,2-dichlorovinyl dimethyl phosphate, an organophosphate insecticide, is widely used for the control of agricultural, industrial, and domestic pests (fig. 1) [1]. dichlorvos is administered orally as an anthelmintic to swine, horses, cats, and dogs. it is applied by dermal application to cattle, goats, sheep, swine, and chickens to control fleas, flies, and mites. cucumbers, radishes, lettuce, and tomatoes are treated with dichlorvos in greenhouses. aerosols and strips are used for t ... | 1981 | 7009035 |
| host susceptibility of the papaya mosaic virus in sri lanka. | 75 plant species from 11 families were tested in sri lanka for their susceptibility to transferring the papaya mosaic virus. after inoculation with this virus, six species, cucurbita pepo, cucumis sativus, nicotiana tabacum, chenopodium amaranticolor, gomphrena globosa and lycopersicum esculentum, developed such symptoms, and after re-isolation from the host plant the virus again infected papaya plants. thus these species are possible alternate hosts of papaya mosaic virus in sri lanka. | 1981 | 7342959 |
| phenotypic comparison between rhizosphere and clinical isolates of burkholderia cepacia. | the phenotypic characteristics of four burkholderia cepacia strains isolated from the rhizosphere and the clinical environment were compared. tests included optimum growth temperature, utilization of carbon sources, production of hcn, indole-3-acetic acid (iaa) and siderophores, proteolytic activity, nitrogen fixation, inhibition of some phytopathogenic fungi, adherence to human mucosal and plant root epithelia, and greenhouse-based plant-growth promotion experiments using cucumber (cucumis sati ... | 1994 | 7517765 |
| a view of plant dehydrins using antibodies specific to the carboxy terminal peptide. | dehydrins are characterized by the consensus kikeklpg amino acid sequence found near the carboxy terminus, and usually repeated from one to many times within the protein. a synthetic peptide containing this consensus sequence was used to produce specific antibodies that recognize dehydrins in a wide range of plants. this range covered two families of monocots, viz. gramineae (hordeum vulgare l., triticum aestivum l., zea mays l., oryza sativa l.) and liliaceae (allium sativa l.), and five famili ... | 1993 | 7693020 |
| cusativin, a new cytidine-specific ribonuclease accumulated in seeds of cucumis sativus l. | dry seeds of cucumis sativus l. were found to contain a heat-sensitive endoribonuclease of a novel type which we have named cusativin. it was purified to apparent electrophoretic homogeneity by chromatography through s-sepharose fast flow, sephadex g-75, cm-sepharose, superdex 75-fplc (fast protein liquid chromatography) and mono s-fplc. it is a single unglycosylated polypeptide chain with an apparent molecular mass (m(r)) of 22900. polyclonal anti-cusativin antibodies raised in rabbits only rea ... | 1994 | 7765423 |
| role of cucumovirus capsid protein in long-distance movement within the infected plant. | direct evidence is presented for a host-specific role of the cucumovirus capsid protein in long-distance movement within infected plants. cucumber (cucumis sativus l.) is a systemic host for cucumber mosaic cucumovirus (cmv). tomato aspermy cucumovirus, strain 1 (1-tav), multiplied to the levels of cmv (i.e., replicated, moved from cell to cell, and formed infectious particles) in the inoculated leaves of cucumbers but was completely unable to spread systemically. the defective long-distance sys ... | 1995 | 7815560 |
| [content of nitrates and nitrites in early vegetables and potatoes sold in the marketplace of białystok in the year 1992]. | nitrate and nitrite were determined in early vegetables and potatoes sold in białystok in 1992. the levels of nitrates and nitrites were determined in 248 samples. nitrate and nitrite were evaluated spectrophotometrically by the method of the griess reaction, after reduction of the nitrates to nitrites, with cadmium dust. high values of nitrate were found in the early vegetables: lettuce, young red beets, radishes, dills, parsley, carrots, cucumbers and potatoes. the results were higher than the ... | 1994 | 7878349 |
| comparison of the czech scrophularia isolate with the italian anagyris strain of scrophularia mottle virus. | scrophularia mottle virus (scmv) was newly found in the czech republic in scrophularia nodosa l. plants. the czech isolate (scmv-c) was serologically identical and similar in symptoms and host range to the italian anagyris strain (scmv-i). nicotiana tabacum l. cv. samsun, nicotiana glutinosa l., nicotiana tabacum l. cv. white burley, physalis floridana rybd. and cucumis sativus l. are described as new host plants of scmv. double-stranded rna patterns and the isoelectric point of this virus are c ... | 1994 | 8067309 |
| "erwinia nulandii" is a subjective synonym of erwinia persicinus. | the organism named "erwinia nulandii" was isolated in 1979 from bean seeds and was described in 1981, but the name was never validated. the results of biochemical tests and membrane protein profile and dna relatedness studies indicated that this name is synonymous with erwinia persicinus, a validly published name for a species previously isolated from, but not shown to be pathogenic for, tomatoes, bananas, and cucumbers. pathogenicity tests revealed that all e. persicinus strains, including an " ... | 1994 | 8186091 |
| distribution and properties of major ribosome-inactivating proteins (28 s rrna n-glycosidases) of the plant saponaria officinalis l. (caryophyllaceae). | we have studied the distribution of the protein synthesis inhibitory activity in the tissues of saponaria officinalis l. (caryophyllaceae). seven major saporins, ribosome-inactivating proteins, were purified to apparent homogeneity from leaves, roots and seeds using a new procedure of rips isolation including ion-exchange and hydrophobic chromatography. they all catalysed the depurination of rat liver ribosomes, which generate the endo's diagnostic rrna fragment upon treatment with acid aniline, ... | 1993 | 8218413 |
| survival and growth of escherichia coli o157:h7 on salad vegetables. | the influence of modified-atmosphere packaging, storage temperature, and time on survival and growth of escherichia coli o157:h7 inoculated onto shredded lettuce, sliced cucumber, and shredded carrot was determined. growth of psychotrophic and mesophilic microorganisms and changes in ph and sensory qualities of vegetables, as judged by subjective evaluation, were also monitored. packaging under an atmosphere containing 3% oxygen and 97% nitrogen had no apparent effect on populations of e. coli o ... | 1993 | 8357240 |
| isolation and characterization of a new chromosomal virulence gene of agrobacterium tumefaciens. | a mutant (strain b119) of agrobacterium tumefaciens with a chromosomal mutation was isolated by transposon (tn5) mutagenesis. the mutant exhibited growth rates on l agar and minimal medium (ab) plates similar to those of the parent strain (strain a208 harboring a nopaline-type ti plasmid). the mutant was avirulent on all host plants tested: daucus carota, cucumis sativus, and kalanchoe diagremontiana. the mutant was not impaired in attachment ability to carrot cells. the mutant had one insertion ... | 1993 | 8491736 |
| anti-hyperglycemic effect of some edible plants. | the anti-hyperglycemic effect of 12 edible plants was studied on 27 healthy rabbits, submitted weekly to subcutaneous glucose tolerance tests after gastric administration of water, tolbutamide or a traditional preparation of the plant. tolbutamide, cucurbita ficifolia, phaseolus vulgaris, opuntia streptacantha, spinacea oleracea, cucumis sativus and cuminum cyminum decrease significantly the area under the glucose tolerance curve and the hyperglycemic peak. brassica oleracea var. botrytis, alliu ... | 1995 | 8569244 |
| cucumber t-complex protein. molecular cloning, bacterial expression and characterization within a 22-s cytosolic complex in cotyledons and hypocotyls. | t-complex protein (tcp) found in mammalian cells and yeast has been proposed as cytosolic folding machinery. we report here the cloning and initial characterization of a plant tcp cdna. cstcp-1 cdna prepared from mrna of cotyledons of germinating cucumber seeds encodes a polypeptide composed of 535 amino acid residues. the 59157-da protein exhibits only 28% identity to both tcp-1p from yeast or and its homolog in arabidopsis thaliana. antibodies raised against the bacterially expressed plant pro ... | 1996 | 8631317 |
| primary structure of omega-hordothionin, a member of a novel family of thionins from barley endosperm, and its inhibition of protein synthesis in eukaryotic and prokaryotic cell-free systems. | a new sulfur-rich basic polypeptide, so called omega-hordothionin, has been isolated from barley endosperm by extractions with nacl and ammonium bicarbonate followed by reverse-phase high performance liquid chromatography. purified omega-hordothionin was found to be homogeneous by sds/polyacrylamide gel electrophoresis, n-terminal amino-acid sequencing and electrospray-ionization mass spectrometric analysis. the complete primary structure of omega-hordothionin was determined by automatic degrada ... | 1996 | 8706720 |
| hemolytic activities of triterpene glycosides from the holothurian order dendrochirotida: some trends in the evolution of this group of toxins. | hemolysis and k+ loss from mouse erythrocytes, induced by triterpene glycosides and their derivatives from this order of sea cucumbers were studied. sulfate groups, attached to position 4 of the first xylose residue and to position 6 of the third glucose residue of the branched pentaosides, having 3-o-methyl-groups in terminal monosaccharide moieties increase k+ loss. a sulfate group at c-4 of the first xylose residue increases the hemolytic activity while a sulfate at c-6 of the third monosacch ... | 1996 | 8735247 |
| association of geminivirus infection with yellow green mosaic disease of cucumis sativus: diagnosis by nucleic acid probes. | geminivirus association with yellow mosaic disease of c. sativus has been investigated by dot/slot blot hybridization tests using nucleic acid probe derived from dna-b of indian tomato leaf curl geminivirus. the disease was transmitted experimentally on c. sativus by whitefly, bemisia tabaci. some weeds were also found to harbour geminivirus infection in dot/slot blot hybridization tests. | 1996 | 8792653 |
| aflatoxin-producing strains of aspergillus flavus in the mould flora of the different greenhouse substrates for the cultivation of cucumber (cucumis sativus, l.). | fungal contamination in a greenhouse for production of cucumbers (cucumis sativus, l.) was studied. 158 samples of water, sand, air, leaves and fruits were analyzed. 25 different genera were isolated, the most frequent ones being pullularia, aspergillus, paecilomyces, alternaria, fusarium, rhizopus and penicillium. pullularia, aspergillus, paecilomyces and penicillium were present in all types of samples studied. twenty-one strains of a. flavus were identified and only eight of those produced af ... | 1996 | 8796422 |
| a virus-inducible cucumber anionic peroxidase has a serological counterpart in different plant species. | a highly specific rabbit antiserum raised against anionic peroxidase (prx) purified from cucumber (cucumis sativus l., cv. laura) cotyledons infected with tobacco necrosis virus (tnv) was used to further investigate the antigenic relatedness of peroxidases in the selected plant species. immunoblot analysis using native polyacrylamide electrophoresis (page) revealed that the antiserum recognized only fast-moving anionic prx isoenzymes and that cross- reacting protein bands were present in five pl ... | 1996 | 8891090 |
| lipid body lipoxygenase characterized by protein fragmentation, cdna sequence and very early expression of the enzyme during germination of cucumber seeds. | lipid bodies are cellular compartments containing triacylglycerols. they are encompassed by a phospholipid monolayer and decorated with characteristic proteins. in plants, lipid bodies are synthesized during seed formation but acquire new proteins during seed germination. in germinating cucumber (cucumis sativus) seeds, the set of newly synthesized proteins appearing in the lipid bodies at the early stage of triacylglycerol mobilization comprises a special form of lipoxygenase. we isolated the l ... | 1996 | 8898881 |
| cloning, expression, and spectroscopic characterization of cucumis sativus stellacyanin in its nonglycosylated form. | the cdna encoding the 182 amino acid long precursor stellacyanin from cucumis sativus was isolated and characterized. the protein precursor consists of four sequence domains: i, a 23 amino acid hydrophobic n-terminal signal peptide with features characteristic of secretory proteins; ii, a 109 amino acid copper-binding domain; iii, a 26 amino acid hydroxyproline- and serine-rich peptide characteristic of motifs found in the extension family, extracellular structural glycoproteins found in plant c ... | 1996 | 8931137 |
| [fungicidal activity of imbricin and its salts]. | the fungicidal activity of imbricin and its salts against fungal phytopathogens was studied. the biological tests revealed that the compounds had a fungicidal activity and inhibited the development of a number of plant diseases. imbricin showed a marked fungicidal activity when used as a disinfectant of wheat seeds and in the control of root rot of cucumbers. it was demonstrated that imbricin and its salts had the growth regulating activity in plant cell culture. | 1996 | 9054323 |
| serological relationships between tobacco necrosis virus-inducible cucumber peroxidase and corresponding peroxidase isoenzymes in other cucurbitaceae. | pathogenesis-related peroxidases (prxs), found in leaves of different cucurbits infected with tobacco necrosis virus (tnv), were compared serologically using a highly specific rabbit antiserum raised against prx purified from tnv-infected cotyledons of cucumis sativus l., cv. laura. after native polyacrylamide gel electrophoresis (page) of intercellular washing fluid extracts from a large number of species of this family, immunoblot analysis revealed the presence of cross-reacting protein bands ... | 1997 | 9199712 |
| identification and partial characterization of beta-1,3-glucanase from virus-infected cucumber cotyledons. | one of the five "pathogenesis-related" (pr) proteins known to accumulate in cucumis sativus l. cv. laura and to react hypersensitively to tobacco necrosis virus (tnv) was shown to to have beta-1,3-glucanase activity. the tnv-induced acidic beta-1,3-glucanase activity increased 6-fold after infection and had extracellular localization and estimated m(r) of 25,700. the beta-1,3-glucanase activity was investigated with a new method of activity staining using a conjugated substrate in overlay gels. ... | 1997 | 9199713 |
| intra- and extracellular isoforms of pr-3 class chitinase in virus-infected cucumber plants. | cucumber (cucumis sativus l. cv. laura) contains three different isoforms of chitinase (ec 3.2.1.14), thought to be involved in the defense against a pathogen. using a highly specific rabbit antiserum raised against a predominant, extracellularly localized, virus-inducible acidic chitinase (p28), two additional enzyme isoforms with differential mode of compartmentalization were identified. immunoblot analysis of the fractionated plant extracts separated by denaturing and native (anodic and catho ... | 1997 | 9219636 |
| origin of the epidermis in parasitic platyhelminths. | the epidermis of members of the major parasitic taxon neodermata is distinctive among flatworms, being a syncytial, insunk, non-ciliated epidermis that develops through a wholesale replacement of larval epidermis at metamorphosis when the larva attacks a host. how it arose in evolution from what must have been a turbellarian-like ancestor is not immediately evident. while many turbellarian flatworms have also adopted a symbiotic way of life, the literature on ultrastructure of epidermis in these ... | 1997 | 9229254 |
| a bacillus subtilis gene encoding a protein similar to nucleotide sugar transferases influences cell shape and viability. | bacillus subtilis gene ypfp, which is located at 196 degrees on the genetic map, shows similarity to both the monogalactosyldiacylglycerol synthase gene of cucumis sativus, which encodes a galactosyltransferase, and the murg genes of b. subtilis, escherichia coli, haemophilus influenzae, and synechocystis sp. strain pcc6803, which encode n-acetylglucosaminyltransferases involved in peptidoglycan biosynthesis. cells containing a null mutation of ypfp are shorter and rounder than wild-type cells d ... | 1997 | 9244290 |
| immunological identification of a basic homologue to acidic virus-inducible cucumber peroxidase. | eight basic, fast-moving pathogenesis-related peroxidase pr-prx isoenzymes were found to accumulate in cotyledons of cucumber (cucumis sativus l., cv.laura) reacting hypersensitively to tobacco necrosis virus (tnv). these isozymes were provisionally designated a, b, c, d, e, f, and g in the order of decreasing electrophoretic mobility in native cathodic polyacrylamide gels. besides the differential mode of compartmentalization, some of these isoenzymes serologically cross-reacted with the antise ... | 1997 | 9385401 |
| first report of a human isolate of erwinia persicinus. | erwinia persicinus was first described in 1990 after being isolated from a variety of fruits and vegetables, including bananas, cucumbers, and tomatoes. in 1994, it was shown to be the causative agent of necrosis of bean pods. we now report the first human isolate of e. persicinus. the strain was isolated from the urine of an 88-year-old woman who presented with a urinary tract infection. by the hydroxyapatite method, dna from this strain was shown to be 94.5% related at 60 degrees c and 86% rel ... | 1998 | 9431957 |
| development of a model for evaluation of microbial cross-contamination in the kitchen. | foods can become contaminated with pathogenic microorganisms from hands, the cutting board, and knives during preparation in the kitchen. a laboratory model was developed to determine occurrence of cross-contamination and efficacy of decontamination procedures in kitchen food-handling practices. enterobacter aerogenes b199a, an indicator bacterium with attachment characteristics similar to that of salmonella spp., was used. chicken meat with skin inoculated with 10(6) cfu of e. aerogenes b199a/g ... | 1998 | 9713754 |
| fatty acid alpha-oxidation of tetradecylthioacetic acid and tetradecylthiopropionic acid in cucumber (cucumis sativus). | fatty acid alpha-oxidation in cucumber (cucumis sativus) involves enzymatic conversion of long-chain cn-fatty acids to the c(n-1)-aldehyde and co2. however, the mechanism of this process is not well understood. in this study, the alpha-oxidation of the fatty acid analogues tetradecylthioacetic acid (tta) and tetradecylthiopropionic acid (ttp) with a sulphur atom substituting the methylene group in positions 3 and 4, respectively, was investigated and compared to palmitic acid. both [1-14c]tta an ... | 1998 | 9795197 |
| characterization of a cdna homologous to carotenoid-associated protein in citrus fruits. | a cdna (citpap) homologous to a gene encoding for cucumis sativus carotenoid-associated protein (chrc) has been isolated from satsuma mandarin (citrus unshiu marc.). unlike chrc whose expression was limited only in mature fruits (containing chromoplasts), citpap transcripts were detected in all the tissues examined including fruits, flowers and leaves. in this respect, citpap was rather close to a gene encoding for pepper plastid-lipid-associated protein (pap), which exhibits ubiquitous expressi ... | 1998 | 9804984 |
| biochemical analysis of plant protection afforded by a nonpathogenic endophytic mutant of colletotrichum magna | a nonpathogenic mutant of colletotrichum magna (path-1) was previously shown to protect watermelon (citrullus lanatus) and cucumber (cucumis sativus) seedlings from anthracnose disease elicited by wild-type c. magna. disease protection was observed in stems of path-1-colonized cucurbits but not in cotyledons, indicating that path-1 conferred tissue-specific and/or localized protection. plant biochemical indicators of a localized and systemic (peroxidase, phenylalanine ammonia-lyase, lignin, and ... | 1999 | 9952476 |
| identification of proteins associated with plastoglobules isolated from pea (pisum sativum l.) chloroplasts. | plastoglobules are conspicuous lipid-containing structures in the chloroplast stroma and are thought to serve as lipid reservoirs for thylakoid membranes. plastoglobules also contain low levels of proteins. we have purified plastoglobules from a pea chloroplast membrane fraction. sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis of the purified plastoglobules revealed more than a dozen distinct polypeptides that we propose to term plastoglobulins. for one of the proteins, termed ... | 1999 | 10213003 |
| biocontrol strain pseudomonas sp. w34: specific detection and quantification in the rhizosphere of cucumis sativus with a dna probe and genotypic characterization by dna fingerprinting. | a dna probe specific for biocontrol strains of pseudomonas was produced by screening randomly amplified polymorphic dna (rapd) pcr fragments. specificity of the probe was assessed by dot blot and colony hybridization. it was used to specifically determine the population of these strains on roots of cucumis sativus cv. delikatess. two polymorphic rapd fragments of 750 bp, and 550 bp showed identical specificity. the biocontrol strain pseudomonas sp. w34 was shown to be competitive in the rhizosph ... | 1999 | 10431388 |
| utilization of heterologous siderophores enhances levels of iron available to pseudomonas putida in the rhizosphere. | pseudomonas spp. have the capacity to utilize siderophores produced by diverse species of bacteria and fungi, and the present study was initiated to determine if siderophores produced by rhizosphere microorganisms enhance the levels of iron available to a strain of pseudomonas putida in this natural habitat. we used a previously described transcriptional fusion (pvd-inaz) between an iron-regulated promoter (pvd) and the ice nucleation reporter gene (inaz) to detect alterations in iron availabili ... | 1999 | 10583989 |
| uptake of dioxin-like compounds from sewage sludge into various plant species--assessment of levels using a sensitive bioassay. | a bioassay for the detection of dioxin-like compounds was used to estimate uptake of dioxin-like compounds in carrots, oil seed rape seeds, zucchinis and cucumbers grown in soil amended with sewage sludge from swedish sewage treatment plants (stp). this sensitive bioassay is based on 7-ethoxyresorufin o-deethylase (erod)-induction in cultured chicken embryo livers and reflects the combined biological effect of all dioxin-like compounds in a sample, including ones that seldom are analyzed. the bi ... | 2000 | 10739061 |
| induction and distribution of amylolytic activity in cucumis sativus l. in response to virus infection. | inoculation of cucumber (cucumis sativus l. cv. laura) cotyledons with tobacco necrosis virus (tnv) causes both qualitative and quantitative changes in the total and fractionated protein extracts as well as in amylolytic activity. using a specific test it was demonstrated that the virus infection strongly enhances a major band (rf 0.0645) of amylolytic activity, predominantly located in apoplast space. the accumulation of this extracellular amylolytic activity is regulated by a time-dependent ma ... | 1999 | 10749368 |
| the lupinus albus class-iii chitinase gene, if3, is constitutively expressed in vegetative organs and developing seeds. | a cdna fragment encoding a lupinus albus. l. class-iii chitinase, if3, was isolated, using a cdna probe from cucumis sativus l., by in-situ plaque hybridization from a cdna library constructed in the uni-zap xr vector, with mrnas isolated from mature lupin leaves. the cdna had a coding sequence of 293 amino acids including a 27-residue n-terminal signal peptide. a class-iii chitinase gene was detected by southern analysis in the l. albus genome. western blotting experiments showed that the if3 p ... | 2000 | 10787047 |
| expression of cucumber lipid-body lipoxygenase in transgenic tobacco: lipid-body lipoxygenase is correctly targeted to seed lipid bodies. | a particular isoform of lipoxygenase (lox, ec 1.13.11.12) localized on lipid bodies has been shown by earlier investigations to play a role during seed germination in initiating the mobilization of triacylglycerols. on lipid bodies of germinating cucumber (cucumis sativus l.) seedlings, the modification of linoleoyl moieties by this lox precedes the hydrolysis of the ester bonds. we analyzed the expression and intracellular location of this particular lox form in leaves and seeds of tobacco (nic ... | 2000 | 10805441 |
| [assessment of the impact of the biological rodenticide biorat in populations of rodents settled in several crops of the republic of costa rica]. | the impact of biological rodenticide biorat on populations of rodents settled in some crops was determined; these crops are highly infested with sigmodon hispidus, a very common vector in various central american countries. the trapping technique and the appraisal of damages allowed to calculate the index of infestation which ranged from 75 to 90 percent in three crops. twenty for to 36 kg of biorat were applied to 6 ha planted with cucumis sativus (cucumber), ipomoea batata (sweet potato) and a ... | 1999 | 10887586 |
| control of pythium root rot on hydroponically grown cucumbers with silver-coated cloth. | silver-coated cloth (scc) effectively controlled root rot that was caused by pythium aphanidermatum in hydroponically grown cucumber plants. the presence of scc in the hydroponic solution reduced the root rot from 100% to 10% 20 days after inoculation with zoospores of p. aphanidermatum. it was suggested that the inhibition of scc was caused not only by the silver ion dissolved from scc, but also by the metallic silver and silver compounds formed on the surface of the root. | 2000 | 10945273 |
| interaction between zucchini yellow mosaic potyvirus rna-dependent rna polymerase and host poly-(a) binding protein. | viral replication depends on compatible interactions between a virus and its host. for rna viruses, the viral replicases (rna-dependent rna polymerases; rdrps) often associate with components of the host translational apparatus. to date, host factors interacting with potyvirus replicases have not been identified. the potyviridae, which form the largest and most economically important plant virus family, have numerous similarities with the animal virus family, the picornaviridae. potyviruses have ... | 2000 | 10998341 |
| a genetic map of cucumber composed of rapds, rflps, aflps, and loci conditioning resistance to papaya ringspot and zucchini yellow mosaic viruses. | the watermelon strain of papaya ringspot virus (prsv-w) and zucchini yellow mosaic virus (zymv) are potyviruses that cause significant disease losses in cucumber. resistances have been identified primarily in exotic germplasm that require transfer to elite cultivated backgrounds. to select more efficiently for virus resistances, we identified molecular markers tightly linked to prsv-w and zymv resistances in cucumber. we generated f6 recombinant inbred lines (rils) from a cross between cucumis s ... | 2000 | 11195331 |
| biochemical characterization of the 2-ketoacid reductases encoded by ycdw and yiae genes in escherichia coli. | glyoxylate is an important intermediate of the central microbial metabolism formed from acetate, allantoin or glycolate. depending on the physiological conditions, glyoxylate is incorporated into the central metabolism by the combined actions of the activity of malate synthase and the d-glycerate pathway, or alternatively it can be reduced to glycolate by constitutive glyoxylate reductase activity. at present no information is available on this latter enzyme in escherichia coli, although similar ... | 2001 | 11237876 |
| biogenic amines in packed table olives and pickles. | the content of biogenic amines in different commercial preparations of table olives and other pickled foods was determined. concentration of amines in packed table olives, capers, caperberries, and cucumbers was less than 60 mg of total biogenic amines per kg of fruit, and, therefore, these products represent no risk to human health. the highest concentrations of putrescine (50 mg/kg) and histamine (38 mg/kg) were found in untreated natural black olives and caperberries, respectively. canned rip ... | 2001 | 11252482 |
| two new cytotoxic and virucidal trisulfated triterpene glycosides from the antarctic sea cucumber staurocucumis liouvillei. | two new trisulfated triterpene glycosides, liouvillosides a (1) and b (2), have been isolated from the antarctic sea cucumber staurocucumis liouvillei. their structures have been elucidated by spectroscopic analysis (nmr and fabms) and chemical transformations. liouvillosides a (1) and b (2) are two new examples of a small number of trisulfated triterpene glycosides from sea cucumbers belonging to the family cucumariidae. both glycosides were found to be virucidal against herpes simplex virus ty ... | 2001 | 11421733 |
| [use of highly dispersed materials for culturing and isolation of granular agrobacterium radiobacter preparations ]. | the effects of synthetic and natural high-dispersion materials on the growth of agrobacterium radiobacter were studied. natural minerals montmorillonite and palygorskite (10 g/l nutrient medium) were more potent than high-dispersion silica and its modified forms in stimulating growth of agrobacterium radiobacter. the interaction of agrobacterium radiobacter with clay minerals increased the survival rate of bacteria at supraoptimal temperatures. we elaborated new granular bacterial preparation, w ... | 2001 | 11443910 |
| how species-specific is the phoretic relationship between the broad mite, polyphagotarsonemus latus (acari: tarsonemidae), and its insect hosts? | broad mite, polyphagotarsonemus latus (acari: tarsonemidae), is a serious plant pest in tropical and subtropical regions. phoretic associations between broad mite and two genera of whiteflies (insecta: homoptera: aleyrodidae). namely bemisia and trialeurodes, have been reported from different parts of the world. our purpose was to determine the specificity of the association between the mite and its phoretic hosts. two host plants, potatoes and cucumbers, were used to study these relationships i ... | 2001 | 11523917 |
| acylated flavone c-glycosides from cucumis sativus. | leaves of cucumis sativus plants treated with silicon and infected with sphaerotheca fuliginea yielded five new acylated flavone c-glycosides identified as isovitexin 2"-o-(6"'-(e)-p-coumaroyl)glucoside (6), isovitexin 2"-o-(6"'-(e)-p-coumaroyl)glucoside-4'-o-glucoside (7), isovitexin 2"-o-(6"'-(e)-feruloyl)glucoside-4'-o-glucoside (11), isoscoparin 2"-o-(6"'-(e)-p-coumaroyl)glucoside (9), and isoscoparin 2"-o-(6"'-(e)-feruloyl)glucoside-4'-o-glucoside (12). the known flavone-glycosides isovitex ... | 2001 | 11524127 |
| spectral quality may be used to alter plant disease development in celss. | plants were grown under light emitting diode (led) arrays with different spectral qualities to determine the effects of light on the development of tomato mosaic virus (tomv) in peppers and powdery mildew on cucumbers. one led array supplied 100% of the photosynthetic photon flux (ppf) at 660 nm, a second array supplied 90% of the ppf at 660 nm and 10% at 735 nm, and a third array supplied 98% of the ppf at 660 nm with 2% in the blue region (380-500 nm) supplied by blue fluorescent lamps. contro ... | 1994 | 11540212 |
| spectral quality affects disease development of three pathogens on hydroponically grown plants. | plants were grown under light-emitting diode (led) arrays with various spectra to determine the effects of light quality on the development of diseases caused by tomato mosaic virus (tomv) on pepper (capsicum annuum l.), powdery mildew [sphaerotheca fuliginea (schlectend:fr.) pollaci] on cucumber (cucumis sativus l.), and bacterial wilt (pseudomonas solanacearum smith) on tomato (lycopersicon esculentum mill.). one led (660) array supplied 99% red light at 660 nm (25 nm bandwidth at half-peak he ... | 1997 | 11540250 |
| a new approach for weed control in a cucurbit field employing an attenuated potyvirus-vector for herbicide resistance. | expression of bar, a phosphinothricin acetyltransferase, in plant tissues, leads to resistance of these plants to glufosinate ammonium based herbicides. we have created a bar expressing, attenuated zucchini yellow mosaic potyvirus-vector, agii-bar, to enable herbicide use in cucurbit fields. the parental vector, zymv-agii, has been rendered environmentally safe by both disease-symptom attenuation and aphid-assisted virus transmission abolishment. the recombinant agii-bar virus-encoding cdna, whe ... | 2001 | 11604171 |
| physiological activity of some organophosphorous compounds and their influence on mechanical properties of erythrocytes. | hemolysis and fluidization of erythrocytes (rbc) membranes by some newly synthesized aminophosphonates as well as their potency to induce electrolyte efflux from cucumber (cucumis sativus cv "wisconsin") cotyledons were studied. also, the chlorophyll content in aminophosphonate-treated cotyledons was affected. the compounds studied differed mainly in hydrophobicity of their substituents at the carbon, nitrogen and phosphorus atoms. it was found that aminophosphonate potency to fluidize rbc membr ... | 2001 | 11837691 |
| polyphenol increases in safflower and cucumber seedlings exposed to strong visible light with limited water. | to assess effects of the environmental stress on polyphenol compounds (polyphenols) in plants, the polyphenol contents were investigated in the seedlings of safflower (carthamus tinctrius l.) and cucumber (cucumis sativus l.) grown under three types of growth conditions: control; light stress, irradiated with strong light in the visible wavelength range; and light/water stress, irradiated with strong visible light with a limited water supply. the total polyphenol contents and the amounts of the ... | 2002 | 11866121 |
| extracellular freezing in leaves of freezing-sensitive species. | low-temperature scanning-electron microscopy was used to study the freezing of leaves of five species that have no resistance to freezing: bean (phaseolus vulgaris l.), tobacco (nicotiana tabacum l.), tomato (lycopersicon esculentum l.), cucumber (cucumis sativus l.), and corn (zea mays l.). in the leaves of the four dicotyledonous species, ice was extracellular and the cells of all tissues were collapsed. in contrast, in maize leaves ice was extracellular in the mesophyll, and these cells were ... | 2002 | 11882950 |
| [comparative study of cytotoxic and hemolytic effects of triterpenoids isolated from ginseng and sea cucumber]. | specific features of cytotoxic (against tumor cells), hemolytic, and liposomal (effect on permeability) activities of triterpenoids isolated from sea cucumbers and ginseng roots were studied. it was shown that oleanolic acid, protopanaxatriol, and protopanaxadiol at 5 to 20 micrograms/ml inhibited the growth of tumor cells, while at doses up to 100 micrograms/ml, they did not induce hemolysis or changes in liposome permeability. monoglucosides of protopanaxadiol, rh2, rg3, and substance k exerte ... | 2002 | 11963541 |
| arbuscular mycorrhizal fungus-promoted accumulation of two new triterpenoids in cucumber roots. | cucumber (cucumis sativus l.) roots were analyzed by hplc and tlc for their levels of secondary metabolites upon inoculation with the arbuscular mycorrhizal fungus, glomus caledonium. three compounds in etoac extracts from the mycorrhizal roots showed significant increases six weeks after inoculation. these compounds were isolated by column chromatography and determined to be two novel triterpenes, 2beta-hydroxybryonolic acid (2beta,3beta-dihydroxy-d:c-friedoolean-8-en-29-oic acid) and 3beta-bry ... | 2002 | 12036048 |
| cytotoxic and antifungal triterpene glycosides from the patagonian sea cucumber hemoiedema spectabilis. | two new sulfated triterpene glycosides, hemoiedemosides a (1) and b (2), have been isolated from the patagonian sea cucumber hemoiedema spectabilis. their structures have been established by a combination of spectroscopic analysis (nmr and fabms) and chemical transformations. both glycosides present the same aglycon and differ in the degree of sulfation of the tetrasaccharide chain. hemoiedemoside b (2) is a new example of a small number of trisulfated triterpene glycosides from sea cucumbers be ... | 2002 | 12088428 |
| allelopathic potential and chemical constituents of volatiles from ageratum conyzoides under stress. | the allelopathic potential of ageratum conyzoides was investigated under different environmental stress conditions, including nutrient deficiency. physical damage, 2.4-d treatment, competition with bidens pilosa, infection with erysiphe cichoracearum, and feeding by aphiids gossypii. the inhibitory effects of a. conyzoides volatiles on peanut (arachis hypogaea), redroot amaranth (amaranthus retroflexus), cucumber (cucumis sativus), and ryegrass (lolium multiforum) increased when plants were grow ... | 2002 | 12184395 |
| [characteristics of functional and nutritious soilless culture substrate for vegetables]. | a functional and nutritious substrate for soilless culture, which consists of peanut shell, sawdust, vermiculite, chicken manure, coal cinder, etc, was used to grow cucumbers, tomatoes and peppers in this experiment. the results showed that the substrate was rich in organic matter, n, available p and k, in which the nutrients were basically in balance for three vegetables. high content of microorganisms and high activity of soil enzymes were propitious to the transformation of organic components ... | 2002 | 12222046 |
| evidence of phytoalexins in cucumber leaves infected with powdery mildew following treatment with leaf extracts of reynoutria sachalinensis. | phenolic compounds extracted from cucumber (cucumis sativus l.) leaves were separated and analyzed for their differential presence and fungitoxicity in relation to a prophylactic treatment with milsana (compo, munster, germany) against powdery mildew (sphaerotheca fuliginea). based on our extraction and purification procedures, at least eight separate phenolic compounds with antifungal activity were identified as intrinsic components of cucumber plants. of these compounds, six displayed a signif ... | 1997 | 12223638 |
| survival of the rhizosphere-competent biocontrol strain pseudomonas fluorescens nbri2650 in the soil and phytosphere. | pseudomonas fluorescens nbri2650 was isolated after screening 360 bacterial strains from the rhizosphere of chickpea (cicer arietinum l.) grown in fungal-disease-suppressive field soil. the strain was selected because of its high rhizosphere competence and ability to inhibit the growth of fusarium oxysporum f.sp. ciceri, rhizoctonia bataticola, and pythium sp. under in vitro conditions. survival and colonization of nbri2650 in the phytosphere of chickpea, cotton (gossypium hirsutum l.), cucumber ... | 2002 | 12224558 |
| competence for elicitation of h2o2 in hypocotyls of cucumber is induced by breaching the cuticle and is enhanced by salicylic acid. | to study h2o2 production, the epidermal surfaces of hypocotyl segments from etiolated seedlings of cucumber (cucumis sativus l.) were gently abraded. freshly abraded segments were not constitutively competent for rapid h2o2 elicitation. this capacity developed subsequent to abrasion in a time-dependent process that was greatly enhanced in segments exhibiting an acquired resistance to penetration of their epidermal cell walls by colletotrichum lagenarium, because of root pretreatment of the respe ... | 1996 | 12226186 |