occurrence of patulin in fruit and different varieties of apples and pears suffering from brown rot, patulin was found in about 50 p. 100 of samples investigated (about 120). patulin levels as high as 1 g/kg rotten material were found 2-3 days after the fruit was removed from the cold store where it had been stored for 5 months. a significant diffusion into the healthy plant tissue was not observed in apples, but in peaches, tomatoes and pears. vegetable products with natural patulin content: apples, pears, peaches, apricots, ...1977613910
[patulin in foods of vegetable origin. 2. several kinds of fruit and vegetables and fruit and vegetable products (author's transl)].patulin was found in fruit with spontaneous brown rot (bananas, pineapples, grapes, peaches, apricots) as well as in moldy compots and in sallow-thorn juice. fruit, vegetables and fruit and vegetable product were artificially infected with penicillium expansum, p. urticae and byssochlamys nivea; patulin was subsequently found in peaches, apricots, greengages, bananas, strawberries, honeydew melons, tomatoes, red and green paprika, cucumbers and carrots; in several kinds of compot, in tomato juic ...1977857490
partial characterization of the translational inhibitor present in seeds of cucumis melo l. 19921486983
temperature-sensitive replication of cucumber mosaic virus in muskmelon (cucumis melo cv. iroquois), maps to rna 1 of a slow strain.several strains of cucumber mosaic virus have been categorized as either 'fast' or 'slow', based on the time of appearance of symptoms after inoculation onto zucchini squash (curbita pepo cv. black beauty). these strains were examined for their ability to replicate in muskmelon (cucumis melo cv. iroquois) at elevated temperatures. all of the fast strains were able to replicate at 37 degrees c in muskmelon, whereas all of the slow strains were unable to replicate to detectable levels at 37 degree ...19911856702
[characterization of xanthomonas campestris pv. cucurbitae, causal agent of the bacterial spot of squash].bacterial leaf spot of squash was characterized for the first time in argentina. cultural, physiological, morphological and cross-infection tests on cucurbitaceae showed that the pathogen was xanthomonas campestris pv. cucurbitae (bryan) dye. the bacterium isolated from winter squash proved pathogenic for pumpkin, winter squash, cucumber and watermelon but no for muskmelon.19892748850
the effects of fruit juices and fruits on the absorption of iron from a rice meal.the effects of the chemical composition of fruit juices and fruit on the absorption of iron from a rice (oryza sativa) meal were measured in 234 parous indian women, using the erythrocyte utilization of radioactive fe method. the corrected geometric mean fe absorptions with different juices varied between 0.040 and 0.129, with the variation correlating closely with the ascorbic acid contents of the juices (rs 0.838, p less than 0.01). ascorbic acid was not the only organic acid responsible for t ...19873593665
pharmacodynamic investigations into the diuretic activity of cucumis melo seed (ether extract). 19705506675
cusativin, a new cytidine-specific ribonuclease accumulated in seeds of cucumis sativus l.dry seeds of cucumis sativus l. were found to contain a heat-sensitive endoribonuclease of a novel type which we have named cusativin. it was purified to apparent electrophoretic homogeneity by chromatography through s-sepharose fast flow, sephadex g-75, cm-sepharose, superdex 75-fplc (fast protein liquid chromatography) and mono s-fplc. it is a single unglycosylated polypeptide chain with an apparent molecular mass (m(r)) of 22900. polyclonal anti-cusativin antibodies raised in rabbits only rea ...19947765423
a new procedure for the preparation of highly active melonin from the dry seeds of cucumis melo l.melon (cucumis melo l.) dry seeds contain melonin, a protein that strongly inhibits ribosomes from different prokaryotic and eukaryotic sources including those from melon. the protein was purified by a new method to yield highly active and stable protein preparations that involves chromatography through s-sepharose fast flow, cm-sepharose, superdex 75 and mono-s. melonin shows important functional properties: 1) its inhibitory effects on translation were irreversible; 2) it is a single unglycosy ...19957787738
genetic engineering of potyvirus resistance using constructs derived from the zucchini yellow mosaic virus coat protein gene.three versions of the zucchini yellow mosaic virus (zymv) coat protein gene were engineered for expression in plants: the full-length coat protein sequence, the conserved core portion of the gene, and an antisense version. these constructs were introduced into muskmelon (cucumis melo) and tobacco plants (nicotiana tabacum) via agrobacterium tumefaciens-mediated transformation; gene expression was verified by northern and western analysis. transgenic r0 and r1 muskmelon plants expressing the full ...19938324251
isolation of a ripening and wound-induced cdna from cucumis melo l. encoding a protein with homology to the ethylene-forming enzyme.a cdna clone (pmel1) was isolated from a climacteric melon fruit cdna library using the tomato ethylene-forming-enzyme (efe) cdna, ptom13, as a probe. northern analysis of rna isolated from wounded leaf and fruit tissue and from preclimacteric and climacteric pericarp tissue was used to determine the pattern of pmel1 rna expression. pmel1 hybridized to a 1.3-kb transcript in climacteric fruit and wounded leaf tissue but was undetectable in preclimacteric fruit and unwounded leaves. maximal expre ...19938444161
[isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from fusarium oxysporum f.sp. melonis].fusarium oxysporum f.sp. melonis, a pathogen of melon (cucumis melo l.), was grown in shaken cultures at 26 degrees c in a mineral salts medium containing glucose, xylan and apple pectin as carbon sources. the extracellular enzymic complex obtained from these cultures showed lytic activity on plant tissues, causing maceration of melon fruits, potato tubers and carrot roots. protoplasts were isolated from melon fruits when the maceration was carried out under appropriate osmotic conditions. this ...19958850131
characterization of epitopes on zucchini yellow mosaic potyvirus coat protein permits studies on the interactions between strains.monoclonal antibodies (mabs) raised against the coat protein of zucchini yellow mosaic potyvirus (zymv) were characterized by epitope mapping using synthetic oligopeptides. two mutant viruses with a mutation in the amino acid sequence important for epitope recognition in vitro were obtained by site-directed mutagenesis of a full-length cdna of zymv. two mabs, cc11 and dd2, could distinguish specifically between these mutants in mixed infections, or after sequential inoculations of muskmelons. se ...19979267009
cloning, expression, and role in pathogenicity of pg1 encoding the major extracellular endopolygalacturonase of the vascular wilt pathogen fusarium oxysporum.pg1 encoding the major in vitro extracellular endopolygalacturonase of the tomato vascular wilt pathogen fusarium oxysporum f. sp. lycopersici was cloned and sequenced. the deduced mature protein had a calculated molecular mass of 35.5 kda and a pi of 6.2, and showed significant similarity with other fungal endopgs. pg1 mrna was induced in vitro by citrus pectin, tomato vascular tissue, 0.1% d-galacturonic acid, and polygalacturonic acid, and repressed by 1% d-galacturonic acid and 1% glucose. r ...19989450333
endopolygalacturonase pg1 in different formae speciales of fusarium oxysporumpg1, the major endopolygalacturonase of the vascular wilt pathogen fusarium oxysporum, was secreted during growth on pectin by 10 of 12 isolates belonging to seven formae speciales, as determined with isoelectric focusing zymograms and sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels. a southern analysis of genomic dna and pcr performed with gene-specific primers revealed that the pg1 locus was highly conserved structurally in most isolates. two pg1-deficient isolates were identifi ...19989572983
cucumisin-like protease from the latex of euphorbia supina.a protease has been purified from the latex of euphorbia supina rafin by two steps of chromatography. the mr was estimated by sds-page to be 80 kda. its activity was inhibited strongly by diisopropyl fluorophosphate, but not by edta, pepstatin, or cysteine protease inhibitors, indicating that the enzyme is a serine protease. the specificity of the protease is broad, but the preferential cleavage sites were c-terminal sites of hydrophobic amino acid residues. the n-terminal sequence of the first ...200010746875
inhibitory serpins from wheat grain with reactive centers resembling glutamine-rich repeats of prolamin storage proteins. cloning and characterization of five major molecular forms.genes encoding proteins of the serpin superfamily are widespread in the plant kingdom, but the properties of very few plant serpins have been studied, and physiological functions have not been elucidated. six distinct serpins have been identified in grains of hexaploid bread wheat (triticum aestivum l.) by partial purification and amino acid sequencing. the reactive centers of all but one of the serpins resemble the glutamine-rich repetitive sequences in prolamin storage proteins of wheat grain. ...200010874043
comparative study on genomes of two japanese melon necrotic spot virus isolates.nucleotide sequences of the genomes of two japanese melon necrotic spot virus (mnsv) isolates, nh and nk were determined. the open reading frames (orfs) in both genomes encode five proteins: p29 (the pre-readthrough domain of p89), p89 (the readthrough domain of p89 identified as the putative rna-dependent rna polymerase), p14 (the pre-readthrough domain of p7a), p7a (the putative movement protein), and p42 (coat protein, cp). nucleotide and amino acid sequence identities of the five proteins of ...200011332272
cloning and characterization of the gene encoding 3-hydroxy-3-methylglutaryl coenzyme a reductase in melon (cucumis melo l. reticulatus).we have isolated a cdna for cm-hmgr, encoding 3-hydroxy-3-methylglutaryl coenzyme a (hmg-coa) reductase in melon (cucumis melo l. reticulatus; genbank accession no. ab021862). cm-hmgr encodes a polypeptide of 588 amino acids that contains two transmembrane domains and a catalytic domain. database searches revealed that cm-hmgr shows homology to hmg1 (63.7%) and hmg2 (70.3%) of tomato, to hmg1 (77.2%) and hmg2 (69.4%) of arabidopsis thaliana, and to hmgr of tobacco (72.6%). functional expression ...200111370859
amino acid sequences of trypsin inhibitors from the melon cucumis melo.two squash family trypsin inhibitors, cmeti-a and cmeti-b, were isolated from the melon (cucumis melo) seeds, by ion exchange chromatography, gel filtration, affinity chromatography, and high-performance liquid chromatography, and their amino acid sequences were determined. all inhibitors contain 29 amino acid residues including 6 half-cystine residues. they differ by twelve amino acid residues. these polypeptides are strong inhibitors of bovine trypsin, with ki values of 1.6 x 10(-10) m (cmeti- ...19958537309
[host plants of aphis gossypii (aphididae), vector of virus of cucumis melo melon (cucurbitaceae) in costa rica].plant species associated with commercial melon crops and surrounding areas were examined to identity the natural host plants of aphis gossypii glover. the study was conducted in two farms located in different melon production areas and plant life zones of costa rica. plant species diversity, percent coverage and distribution over time were recorded during one year. differences between locations were observed. a total of 86 plant species (49 families) and 72 plant species (40 families) were ident ...200111795159
molecular and genetic characterization of a non-climacteric phenotype in melon reveals two loci conferring altered ethylene response in fruit.fruit ripening and abscission are associated with an ethylene burst in several melon (cucumis melo) genotypes. in cantaloupe as in other climacteric fruit, exogenous ethylene can prematurely induce abscission, ethylene production, and ripening. melon genotypes without fruit abscission or without ethylene burst also exist and are, therefore, non-climacteric. in the nonabscising melon fruit pi 161375, exogenous ethylene failed to stimulate abscission, loss of firmness, ethylene production, and exp ...200212011360
in vitro effect of vegetable and fruit juices on the mutagenicity of 2-amino-3-methylimidazo[4,5-f]quinoline, 2-amino-3,4-dimethylimidazo[4,5-f]quinoline and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline.the antimutagenic potencies of the juices of 28 fruits and 34 vegetables commonly consumed in germany were investigated with respect to the mutagenic activities induced by 2-amino-3-methyl[4,5-f]-quinoline (iq), and in part by 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (meiq) or 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (meiqx) in salmonella typhimurium ta98 and ta100. with iq, weak to strong antimutagenic activities were found in 68% of the fruits and 73% of the vegetables that were tested ...19948206443
characterization of the formae speciales of fusarium oxysporum causing wilts of cucurbits by dna fingerprinting with nuclear repetitive dna sequences.the genetic relatedness of five formae speciales of fusarium oxysporum causing wilts of cucurbit plants was determined by dna fingerprinting with the moderately repetitive dna sequences folr1 to folr4. the four folr clones were chosen from a genomic library made from f. oxysporum f. sp. lagenariae 03-05118. total dnas from 50 strains representing five cucurbit-infecting formae speciales, cucumerinum, melonis, lagenariae, niveum, and momordicae, and 6 strains of formae speciales pathogenic to oth ...19948085813
protease from the sarcocarp of trichosanthes bracteata.a protease has been purified from sarcocarp of trichosanthes bracteata (lam.) voigt by four steps of chromatography. its m(r) was estimated by sds-page to be ca 67,000. the optimum ph of the enzyme was 11 at 35 degrees using casein substrate. the enzyme was strongly inhibited by di-isopropyl fluorophosphate, but not by edta and cysteine protease inhibitors. the oxidized insulin b-chain was cleaved at the peptide bonds of cys7 (so3h)-gly8, glu13-ala14, try16-leu17 by the enzyme for 1 min. the res ...19947764589
identification of platelet inhibitor present in the melon (cucurbitacea cucumis melo).an active fraction was isolated from an aqueous melon extract (cucurbitacea cucumis melo) and was shown that it inhibits human platelet aggregation induced by epinephrine, adp, collagen, thrombin, sodium arachidonate, prostaglandin endoperoxide analogue u-46619 and paf-acether. identification of the active substance as adenosine was indicated by tlc which gave identical rf value compared to adenosine, by the uv spectrum, because the inhibitory effect on platelet aggregation disappeared after the ...19853931281
post-translational modifications in the large subunit of ribulose bisphosphate carboxylase/oxygenase.two adjacent n-terminal tryptic peptides of the large subunit of ribulose bisphosphate carboxylase/oxygenase [3-phospho-d-glycerate carboxy-lyase (dimerizing), ec] from spinach, wheat, tobacco, and muskmelon were removed by limited tryptic proteolysis. characterization by peptide sequencing, amino acid composition, and tandem mass spectrometry revealed that the n-terminal residue from the large subunit of the enzyme from each plant species was acetylated proline. the sequence of the pen ...19892928307
powdery mildew (sphaerotheca fuliginea) resistance in melon is selectable at the haploid level.the major cause of powdery mildew in melons (cucumis melo l.) is the fungus sphaerotheca fuliginea. there are several cultivar- and season-specific races of this fungus. in order to control powdery mildew, it is important to introduce resistance to fungal infection into new cultivars during melon breeding. haploid breeding is a powerful tool for the production of pure lines. in this study, it was investigated whether powdery mildew resistance could be manifested at the haploid level from two dis ...200312598576
blood sugar lowering potentiality of selected cucurbitaceae plants of indian origin.using five experimental models, the blood sugar lowering efficacy of eight plants of cucurbitaceae family has been assessed. the ethanolic extract of cucumis sativus linn, cucumis melo utilissimum roxb, cucumis melo linn, benincasa hispida thunb cogn and tricosanthes anguina nees, when administered in 250 mg/kg dose, orally to rats failed to lower blood sugar or to depress the peak value, after glucose load. however, ethanolic extract of momordica charantia linn plant and coccinia indica whit an ...19892620957
species determination of eggs of opisthorchiid and heterophyid flukes using scanning electron microscopy.eggs of opisthorchiid flukes have very characteristic muskmelon-like surface structure of their shells and can be differentiated using this mark from eggs of heterophyid flukes. surface structure and morphological details in operculum and abopercular end can be observed using scanning electron microscopy. eggs recorded very often in stool of laotians belong mostly to opisthorchis viverrini and sporadically to metagonimus yokogawai.19902337251
toxicity of potassium cyanide added to fresh fruit and investigate the toxicity of potassium cyanide in fresh fruit and juice, male and female wistar rats were orally dosed with fruit homogenates or juices containing 3 x ld50 of potassium cyanide. these were given in single doses at various intervals after spiking. the dosing solutions were analysed for cyanide using a cyanide test kit. there was a good correlation between the toxic signs in rats and the cyanide remaining in dosing solutions. the toxicity of spiked apple and honeydew melon dimini ...19911959821
the complexity of satellite deoxyribonucleic acid in a higher plant.purified satellite dna from melon (cucumis melo) was shown to contain at least two components from thermal-denaturation and renaturation studies. two components were separated after partial renaturation, a fast-renaturing fraction similar in complexity to mouse satellite dna, and one with 6000 times greater complexity. both components renatured very accurately, indicating a minimum of sequence divergence. centrifugation of the purified satellite dna in ag+/cs2so4 gradients resolved two major and ...19751191264
identification of phloem involved in assimilate loading in leaves by the activity of the galactinol synthase promoter.the definition of "minor" veins in leaves is arbitrary and of uncertain biological significance. generally, the term refers to the smallest vein classes in the leaf, believed to function in phloem loading. we found that a galactinol synthase promoter, cloned from melon (cucumis melo), directs expression of the gusa gene to the smallest veins of mature arabidopsis and cultivated tobacco (nicotiana tabacum) leaves. this expression pattern is consistent with the role of galactinol synthase in sugar ...200010889241
cucumber mosaic virus infection affects sugar transport in melon plants.viral infection often affects carbon assimilation and metabolism in host plants. to better understand the effect of cucumber mosaic virus (cmv) infection on sugar transport, carbohydrate levels and the amounts of the various sugars in the phloem sap were determined in infected melon (cucumis melo l.) plants. source leaves infected with cmv were characterized by high concentrations of reducing sugars and relatively low starch levels. the altered level of carbohydrates was accompanied by increased ...200010859189
recovery of surface bacteria from and surface sanitization of cantaloupes.practical, effective methods that could be implemented in a food service establishment (restaurant or delicatessen) for the surface sanitization of cantaloupes were microbiologically evaluated. cantaloupes (cucumis melo l. var. reticulates) were immersed in an inoculum containing salmonella enterica serovar poona or pantoea agglomerans at ca. 10(4) to 10(5) cfu/ml. an efficient method for the recovery of bacteria from the cantaloupe surface was developed and validated. the method consisted of wa ...200314572217
potassium permanganate staining for differentiation the surface morphology of opisthorchis viverrini, haplorchis taichui and phaneropsolus bonnei eggs.potassium permanganate staining method was developed for differentiation opisthorchis viverrini, haplorchis taichui and phaneropsolus bonnei eggs. the surfaces of o. viverrini, h. taichui and p. bonnei eggs stained permanently and temporarily were similar in appearance even the staining procedures were varied both in concentration and time. determined under light microscope set at 400x, all of these eggs were oval-shaped, operculated at one pole and indistinct small knob at posterior end. o. viv ...199910774711
characterization of ripening-regulated cdnas and their expression in ethylene-suppressed charentais melon fruit.charentais melons (cucumis melo cv reticulatus) are climacteric and undergo extremely rapid ripening. sixteen cdnas corresponding to mrnas whose abundance is ripening regulated were isolated to characterize the changes in gene expression that accompany this very rapid ripening process. sequence comparisons indicated that eight of these cdna clones encoded proteins that have been previously characterized, with one corresponding to acc (1-aminocyclopropane-1-carboxylic acid) oxidase, three to prot ...200010712562
a new subtilisin-like proteinase from roots of the dandelion taraxacum officinale webb s. l.a serine proteinase from roots of taraxacum officinale webb s. l. was isolated by affinity chromatography and gel-filtration on superose 6r using fplc. the enzyme is a 67-kd glycoprotein containing 54% carbohydrate which we have named taraxalisin. the substrate specificity of taraxalisin toward synthetic peptides and oxidized insulin b-chain is comparable with that of cucumisin from cucumis melo and the subtilisin-like serine proteinase macluralisin from maclura pomifera. the proteinase is inact ...199910521720
evaluation of botulinal toxin production in packaged fresh-cut cantaloupe and honeydew melons.the ability of clostridium botulinum to produce toxin on cubed, packaged melons was investigated relative to microbial spoilage at various incubation temperatures and in different packaging systems. freshly cut cubes (approximately 2.5 cm3) of cantaloupe and honeydew melons were surface inoculated with a 10 strain mixture of proteolytic and nonproteolytic spores of c. botulinum (10 to 15 cubes per package; approximately 100 total spores per package). to initially evaluate toxin production and sp ...199910456752
polygalacturonase gene expression in ripe melon fruit supports a role for polygalacturonase in ripening-associated pectin disassembly.ripening-associated pectin disassembly in melon is characterized by a decrease in molecular mass and an increase in the solubilization of polyuronide, modifications that in other fruit have been attributed to the activity of polygalacturonase (pg). although it has been reported that pg activity is absent during melon fruit ripening, a mechanism for pg-independent pectin disassembly has not been positively identified. here we provide evidence that pectin disassembly in melon (cucumis melo) may be ...19989625689
poisoning of cattle attributed to cucumis melo ssp agrestis (ulcardo melon). 19958534234
molecular characterization of a melon necrotic spot virus strain that overcomes the resistance in melon and nonhost plants.resistance of melon (cucumis melo l.) to melon necrotic spot virus (mnsv) is inherited as a single recessive gene, denoted nsv. no mnsv isolates described to date (e.g., mnsv-malpha5), except for the mnsv-264 strain described here, are able to overcome the resistance conferred by nsv. analysis of protoplasts of susceptible (nsv/-) and resistant (nsv/nsv) melon cultivars inoculated with mnsv-264 or mnsv-malpha5 indicated that the resistance trait conferred by this gene is expressed at the single- ...200415195949
investigation of volatiles in charentais cantaloupe melons (cucumis melo var. cantalupensis). characterization of aroma constituents in some cultivars.volatile compounds of 15 charentais melon cultivars, known to exhibit differences in their ripening behaviors and in their storage lives (wild, mid, and long shelf life), were investigated. twenty-eight volatiles (11 esters, 8 sulfur compounds, 6 alcohols, and 3 carbonyl compounds) were isolated by direct dichloromethane extraction and analyzed by means of gc-ms and gc-fid. a considerable reduction in the aroma profile was observed for the long shelf life cultivars, in which total volatiles were ...200415237961
eggshell morphology of the small eggs of human trematodes in thailand.light and scanning electron micrographs of opisthorchis viverrini, haplorchis taichui, h. pumilio, a phaneropsolus bonnei, and prosthodendrium molenkampi eggs were studied. under light microscopy. o. viverrini eggs had rough eggshells and prominent shoulders. h. taichui, h. pumilio were similar in shape and had smooth eggshells and prominent shoulders. h. pumilio eggs were bigger than h. taichui eggs. p. bonnei and p. molenkampi eggs had smooth eggshells and indistinct shoulders. p. bonnei eggs ...19911820654
[microbiological stability study of minimally processed cantaloupe (cucumis melo l) by vacuum].in order to design a minimal process for cantaloupe, the water activity of the fruit was reduced until 0.976 through vacuum osmotic dehydration and the optimum combination of microbial preservatives (100 ppm sodium sulfite, 600 ppm potassium sorbate, 0.5% l-ascorbic acid and 1% citric acid), that contributed to minimize (p < 0.05), the content of aerobic mesophilic bacteria, molds and yeast, was determined through a 2k multifactorial design. in the second experimental stage, the conditions previ ...200111678049
comparative mapping of zymv resistances in cucumber (cucumis sativus l.) and melon (cucumis melo l.).zucchini yellow mosaic virus (zymv) routinely causes significant losses in cucumber ( cucumis sativus l.) and melon ( cucumis melo l.). zymv resistances from the cucumber population 'tmg1' and the melon plant introduction (pi) 414723 show different modes of inheritance and their genetic relationships are unknown. we used molecular markers tightly linked to zymv resistances from cucumber and melon for comparative mapping. a 5-kb genomic region (ycz-5) cosegregating with the zym locus of cucumber ...200415340688
examination of bacteriophage as a biocontrol method for salmonella on fresh-cut fruit: a model study.the preparation and distribution of fresh-cut produce is a rapidly developing industry that provides the consumer with convenient and nutritious food. however, fresh-cut fruits and vegetables may represent an increased food safety concern because of the absence or damage of peel and rind, which normally help reduce colonization of uncut produce with pathogenic bacteria. in this study, we found that salmonella enteritidis populations can (i) survive on fresh-cut melons and apples stored at 5 degr ...200111510645
cdna probes for detection of specific dsrnas from the fungal pathogen, monosporascus cannonballus.monosporascus cannonballus is an ascomycete fungus that is the causative agent of monosporascus root rot/vine decline, a serious disease of muskmelon and watermelon. double-stranded rna (dsrna) was identified in approximately 60% of m. cannonballus isolates recovered from infected muskmelon plants in 1993. after repeated laboratory transfer on culture media, the majority of the isolates harboring dsrnas developed degenerate culture phenotypes and showed reduced virulence (hypovirulence) to muskm ...200010680971
transfer of the yeast salt tolerance gene hal1 to cucumis melo l. cultivars and in vitro evaluation of salt agrobacterium-mediated gene transfer method for production of transgenic melon plants has been optimized. the hal1 gene, an halotolerance gene isolated from yeast, was inserted in a chimaeric construct and joined to two marker genes: a selectable-neomycin phosphotransferase-ii (nptii)-, and a reporter-beta-glucuronidase (gus)-. the entire construct was introduced into commercial cultivars of melon. transformants were selected for their ability to grow on media containing kanamycin. transforma ...19979032977
a soil suspension-plating method of estimating populations of fusarium oxysporum f. melonis in muskmelon wilt soils. 196214006148
strain-specific and recessive qtls involved in the control of partial resistance to fusarium oxysporum f. sp. melonis race 1.2 in a recombinant inbred line population of melon.fusarium oxysporum f. sp. melonis (fom) causes serious economic losses in melon (cucumis melo l.). two dominant resistance genes have been identified, fom-1 and fom-2, which provide resistance to races 0 and 2 and races 0 and 1, respectively, however fom race 1.2 overcomes these resistance genes. a partial resistance to fom race 1.2 that has been found in some far east accessions is under polygenic control. a genetic map of melon was constructed to tag fom race 1.2 resistance with dna markers on ...200515834544
bacteremia caused by janibacter melonis.we report a case of bacteremia caused by janibacter melonis, a recently described aerobic actinomycete originally isolated from a spoiled oriental melon. our patient's blood culture isolate was identified by partial 16s rrna gene sequencing. this is the first report of the recovery of janibacter species from humans.200516000500
biogenesis of volatile aldehydes from fatty acid hydroperoxides: molecular cloning of a hydroperoxide lyase (cyp74c) with specificity for both the 9- and 13-hydroperoxides of linoleic and linolenic acids.a novel member of the plant cytochrome p450 cyp74 family of fatty acid hydroperoxide metabolizing enzymes has been cloned from melon fruit (cucumis melo). the cdna is comprised of 1,446 nucleotides encoding a protein of 481 amino acids. the homology at the amino acid level to other members of the cyp74 family is 35-50%, the closest relatives being allene oxide synthases. the cdna was expressed in escherichia coli, and the corresponding protein was purified by affinity column chromatography. the ...200111368353
relationship of cell surface charge and hydrophobicity to strength of attachment of bacteria to cantaloupe rind.the cantaloupe melon has been associated with outbreaks of salmonella infections. it is suspected that bacterial surface charge and hydrophobicity may affect bacterial attachment and complicate bacterial detachment from cantaloupe surfaces. the surface charge and hydrophobicity of strains of salmonella, escherichia coli (o157:h7 and non-o157:h7), and listeria monocytogenes were determined by electrostatic and hydrophobic interaction chromatography, respectively. initial bacterial attachment to c ...200212117240
resistance gene homologues in melon are linked to genetic loci conferring disease and pest resistance.genomic and cdna fragments with homology to known disease resistance genes (rgh fragments) were cloned from cucumis melo using degenerate-primer pcr. fifteen homologues of the nbs-lrr gene family have been isolated. the nbs-lrr homologues show high divergence and, based on the partial nbs-fragment sequences, appear to include members of the two major subfamilies that have been described in dicot plants, one that possesses a tir-protein element and one that lacks such a domain. genomic organizati ...200212582612
functional characterization of a melon alcohol acyl-transferase gene family involved in the biosynthesis of ester volatiles. identification of the crucial role of a threonine residue for enzyme activity*.volatile esters, a major class of compounds contributing to the aroma of many fruit, are synthesized by alcohol acyl-transferases (aat). we demonstrate here that, in charentais melon (cucumis melo var. cantalupensis), aat are encoded by a gene family of at least four members with amino acid identity ranging from 84% (cm-aat1/cm-aat2) and 58% (cm-aat1/cm-aat3) to only 22% (cm-aat1/cm-aat4). all encoded proteins, except cm-aat2, were enzymatically active upon expression in yeast and show different ...200516247561
janibacter melonis sp. nov., isolated from abnormally spoiled oriental melon in korea.two gram-positive bacterial strains, cm2104(t) and cm2110, isolated from the inner part of abnormally spoiled oriental melon (cucumis melo) in korea, were subjected to a polyphasic taxonomic study. the cell-wall peptidoglycan of strains cm2104(t) and cm2110 contained meso-diaminopimelic acid as the diagnostic diamino acid. the predominant menaquinone was mk-8(h(4)). the major fatty acids detected in the two strains were iso-c(16 : 0), c(17 : 1)omega8c and c(18 : 1)omega9c or c(17 : 0). the dna g ...200415545420
immunolocalization of a class iii chitinase in two muskmelon cultivars reacting differently to fusarium oxysporum f. sp. melonis.fusarium oxysporum f. sp. melonis is a highly specialized fungus that attacks the root system of melon (cucumis melo l.). in this work the presence of a class iii chitinase was examined by immunological techniques in the root and stem base of a susceptible (cv. galia) and a resistant (cv. bredor) melon during the infection process. by immunolocalization it was not possible to detect the constitutive presence of class iii chitinase in any of the cultivars. however, the immunolabelling appeared in ...200616360800
salicylic acid and ethylene pathways are differentially activated in melon cotyledons by active or heat-denatured cellulase from trichoderma longibrachiatum.infiltration of cellulase (ec from trichoderma longibrachiatum into melon (cucumis melo) cotyledons induced several key defense mechanisms and hypersensitive reaction-like symptoms. an oxidative burst was observed 3 hours after treatment and was followed by activation of ethylene and salicylic acid (sa) signaling pathways leading to marked induction of peroxidase and chitinase activities. the treatment of cotyledons by heat-denatured cellulase also led to some induction of peroxidase an ...200111553761
molecular markers linked to papaya ring spot virus resistance and fusarium race 2 resistance in melon, the fom-1 gene confers monogenic resistance against the soil-borne fungus fusarium oxysporum f. sp. melonis, races 0 and 2, while the closely linked prv gene specifies resistance against the papaya ring spot virus. markers linked to these resistance (r) genes were identified using two recombinant inbred line populations, derived from crosses between cucumis melo vedrantais and c. melo pi 161375, and between c. melo vedrantais and c. melo pi 414723, respectively. using bulked segregant ...200515551034
functional and phylogenetic analyses of a conserved regulatory program in the phloem of minor veins.the minor-vein phloem of mature leaves is developmentally and physiologically distinct from the phloem in the rest of the vascular system. phloem loading of transport sugars occurs in the minor veins, and consistent with this, galactinol synthase is expressed in the minor veins of melon (cucumis melo) as part of the symplastic-loading mechanism that operates in this species. a galactinol synthase promoter from melon drives gene expression in the minor-vein companion cells of both transgenic toba ...200314526110
nucleotide sequences of two korean isolates of cucumber green mottle mosaic virus.the nucleotide sequences of the genomic rnas of cucumber green mottle mosaic virus korean watermelon isolate (cgmmv-kw) and korean oriental melon isolate (cgmmv-kom) were determined and compared to the sequences of other tobamoviruses including cgmmv strains w and sh. each cgmmv isolate had a genome of 6,424 nucleotides. each also had 60 and 176 nucleotides of 5' and 3' untranslated regions (utrs), respectively, and four open reading frames (orf1-4). orfs 1 to 4 encode proteins of 129, 186, 29, ...200314744034
five independent loci each control monogenic resistance to gummy stem blight in melon ( cucumis melo l.).inheritance and segregation analysis demonstrated that five independent genes in melon confer monogenic resistance to foliar infection by the fungal pathogen didymella bryoniae, resulting in the disease known as gummy stem blight (gsb). in this study, two new monogenic sources of gsb resistance were characterized. resistance in cucumis melo pi 482398 was monogenic dominant based on segregation analysis of f(1), f(2) and backcross populations, while resistance in c. melo pi 482399 showed monogeni ...200415067389
isolation and identification of a phosphate deficiency-induced c-glycosylflavonoid that stimulates arbuscular mycorrhiza formation in melon roots.melon (cucumis melo) roots were inoculated with or without the arbuscular mycorrhizal (am) fungus glomus caledonium under low phosphate conditions. high-performance liquid chromatography analysis of the secondary metabolites in butanol extracts from roots revealed that the level of one compound in noninoculated roots showed a significant increase from 30 days postinoculation. no accumulation was observed in mycorrhizal roots and high-phosphate-supplemented roots, indicating that the accumulation ...200212026171
sucrose metabolism in netted muskmelon fruit during development.sugar content and composition are major criteria used in judging the quality of netted muskmelon (cucumis melo l. var reticulatus) fruit. sugar composition and four enzymes of sucrose metabolism were determined in ;magnum 45' muskmelon fruit at 10-day intervals beginning 10 days after pollination (dap) until full-slip (35 dap). sugar content increased in both outer (green) mesocarp and inner (orange) mesocarp between 20 and 30 dap. the major proportion of total increase in sugar was attributed t ...198716665448
pcr identification of salmonella: potential contamination sources from production and postharvest handling of cantaloupes.salmonella is one of the most frequently reported etiological agents in outbreaks of foodborne diseases associated with the consumption of cantaloupes. sensitive and reliable methods for detecting and identifying foodborne microorganisms are needed. the pcr can be used to amplify specific dna fragments and thus to detect and identify pathogenic bacteria. in this study, a pcr method was used to evaluate the incidence of salmonella at cantaloupe production, harvest, and packaging steps, and the re ...200616786866
cloning and expression analysis of a udp-galactose/glucose pyrophosphorylase from melon fruit provides evidence for the major metabolic pathway of galactose metabolism in raffinose oligosaccharide metabolizing plants.the cucurbitaceae translocate a significant portion of their photosynthate as raffinose and stachyose, which are galactosyl derivatives of sucrose. these are initially hydrolyzed by alpha-galactosidase to yield free galactose (gal) and, accordingly, gal metabolism is an important pathway in cucurbitaceae sink tissue. we report here on a novel plant-specific enzyme responsible for the nucleotide activation of phosphorylated gal and the subsequent entry of gal into sink metabolism. the enzyme was ...200616829585
sphingomonas melonis sp. nov., a novel pathogen that causes brown spots on yellow spanish melon fruits.a polyphasic taxonomic study was performed on the phytopathogenic bacterial strains dapp-pg 224(t) and dapp-pg 228, which cause brown spot on yellow spanish melon (cucumis melo var. inodorus) fruits. based on the presence of glucuronosyl ceramide (sgl-1) in cellular lipids, the results of fatty acid analysis and 16s rdna sequence comparison, the strains had been identified as belonging to the genus sphingomonas and as phylogenetically related to sphingomonas mali, sphingomonas pruni and sphingom ...200212508872
binary transformation systems based on 'shooter' mutants of agrobacterium tumefaciens: a simple, efficient and universal gene transfer technology that permits marker gene elimination.a simple transformation procedure with a positive selection scheme using the expression of the isopentenyl transferase ( ipt) gene of transfer dna (t-dna) 'shooter' mutants of agrobacterium tumefaciens was elaborated. after comparing several 'shooter' mutants we found that particular strains frequently produced phenotypically normal shoots after co-culturing with tobacco leaf explants. shoots selected for normal phenotype showed apical dominance and could be rooted with the same efficiency as no ...200312789522
optimizing concentration and timing of a phage spray application to reduce listeria monocytogenes on honeydew melon tissue.a phage cocktail was applied to honeydew melon pieces 1, 0.5, and 0 h before contamination with listeria monocytogenes strain lcdc 81-861 and 0.5, 1, 2, and 4 h after contamination. the phage application was most effective when applied 1, 0.5, or 0 h before contamination with l. monocytogenes, reducing pathogen populations by up to 6.8 log units after 7 days of storage. this indicates that under commercial conditions, if contamination occurs at the time of cutting, phage would have to be applied ...200415330534
a physical map covering the nsv locus that confers resistance to melon necrotic spot virus in melon (cucumis melo l.).melon necrotic spot virus (mnsv) is a member of the genus carmovirus, which produces severe yield losses in melon and cucumber crops. the nsv gene is the only known natural source of resistance against mnsv in melon, and confers protection against all widespread strains of this virus. nsv has been previously mapped in melon linkage group 11, in a region spanning 5.9 cm, saturated with rapd and aflp markers. to identify the nsv gene by positional cloning, we started construction of a high-resolut ...200516052354
ethylene regulation of fruit softening and cell wall disassembly in charentais melon.cell wall disassembly in ripening fruit is highly complex, involving the dismantling of multiple polysaccharide networks by diverse families of wall-modifying proteins. while it has been reported in several species that multiple members of each such family are expressed in the same fruit tissue, it is not clear whether this reflects functional redundancy, with protein isozymes from a single enzyme class performing similar roles and contributing equally to wall degradation, or whether they have d ...200717308329
identification of quantitative trait loci involved in fruit quality traits in melon (cucumis melo l.).two populations [an f(2) and a set of 77 double haploid lines (dhls)] developed from a cross between a 'piel de sapo' cultivar (ps) and the exotic korean accession pi 161375 were used to detect qtls involved in melon fruit quality traits: earliness (ea), fruit shape (fs), fruit weight (fw) and sugar content (ssc); and loci involved in the colour traits: external colour (ecol) and flesh colour (fc). high variation was found, showing transgressive segregations for all traits. the highest correlati ...200414576985
longevity of fusarium oxysporum in soil tube soil tube culture, representatives of three biologic forms of fusarium oxysporum survived unchanged morphologically for 11 years or more. an isolate of the muskmelon wilt fungus remained viable after 17 years' storage in dry air at a temperature of from 3 degrees to 4 degrees c. the surviving unit was found to be the chlamydospore.196117800128
induction of a small heat shock protein and its functional roles in nicotiana plants in the defense response against ralstonia tobacco (nicotiana tabacum), ralstonia solanacearum oe1-1 (rsoe1-1) is pathogenic, whereas r. solanacearum 8107 (rs8107) is nonpathogenic and induces the hypersensitive response (hr). to elucidate the molecular mechanisms of plant-r. solanacearum interactions, we used differential display to isolate a cdna fragment, a6, regulated in tobacco by inoculation with rsoe1-1. the deduced amino acid sequence predicted from full-length a6-cdna showed similarity to small heat shock proteins from arabid ...200717965181
sensory and quality analysis of different melon cultivars after prolonged storage.the purpose of this work was to evaluate the sensory and general quality of four different melon cultivars (cucumis melo l.) immediately after harvest and at the end of storage and marketing simulation. after 16 days of storage at 5 degrees c and additional 3 days at 20 degrees c, only cultivar 'c-8' had a poor general appearance due to significant low firmness and relatively high decay incidence compared to the cultivars '5080', 'ideal' and '7302'. the cultivar '7302' was found to have the high ...200314609087
[effects of exogenous gamma-aminobutyric acid on antioxidant enzyme activity and reactive oxygen content in muskmelon seedlings under nutrient solution hypoxia stress].the effects of exogenous gamma-aminobutyric acid (gaba) on antioxidant defense system and reactive oxygen species (ros) in seedlings of two varieties with different resistance to hypoxia stress were studied under hypoxia stress in a nutrient solution culture system. the results showed that gaba treatments enhanced the activities of antioxidant enzyme including sod (fig. 1), pod (fig. 2), cat (fig. 3), while the concentrations of h2o2 (fig. 4), o(-.)2 (fig. 5), mda (fig. 6) decreased under hypoxi ...200415643085
effect of hand wash agents on controlling the transmission of pathogenic bacteria from hands to food.the goals of this study were to evaluate the effectiveness of two hand wash regimens in reducing transient bacteria on the skin following a single hand wash and the subsequent transfer of the bacteria to a ready-to-eat food item, freshly cut cantaloupe melon. the number of bacteria recovered from hands and the quantity transferred to the melon were significantly less following the use of an antibacterial soap compared with plain soap. the antimicrobial soap achieved > 3-log reductions versus esc ...200718095447
effect of hot water and hydrogen peroxide treatments on survival of salmonella and microbial quality of whole and fresh-cut cantaloupe.cantaloupe melon has been associated with outbreaks of salmonellosis. contamination might be introduced into the flesh from the rind by cutting or by contact of cut pieces with contaminated rinds. our objectives were to investigate the efficacy of hot water or hot 5% hydrogen peroxide treatments in reducing the population of native microflora and inoculated salmonella on cantaloupe rind and transfer to fresh-cut tissue during cutting. whole cantaloupes, inoculated with a cocktail of salmonella s ...200415035354
complete nucleotide sequence of a new isolate of tomato leaf curl new delhi virus infecting cucumber, bottle gourd and muskmelon in thailand. 200818193155
phloem specific expression driven by wheat dwarf geminivirus v-sense promoter in transgenic dicotyledonous species.wheat dwarf geminivirus (wdv) is a single-stranded dna mastrevirus. the large intergenic region (lir) of wdv contains cis-acting elements essential for the replication of the genome as well as for the bidirectional transcription of virus genes. the lir was fused to the gus (uida) reporter gene and the wdv viral sense (v-sense) promoter activity derived from the stable integration of that promoter was analysed in transgenic dicot plants. various dicot species were tested, including nicotiana taba ...200415086824
construction of a fosmid library of cucumber (cucumis sativus) and comparative analyses of the eif4e and eif(iso)4e regions from cucumber and melon (cucumis melo).a fosmid library of cucumber was synthesized as an unrestricted resource for researchers and used for comparative sequence analyses to assess synteny between the cucumber and melon genomes, both members of the genus cucumis and the two most economically important plants in the family cucurbitaceae. end sequencing of random fosmids produced over 680 kilobases of cucumber genomic sequence, of which 25% was similar to ribosomal dnas, 25% to satellite sequences, 20% to coding regions in other plants ...200818273646
reducing salmonella on cantaloupes and honeydew melons using wash practices applicable to postharvest handling, foodservice, and consumer preparation.washing conditions that included a soak or brush scrub were evaluated for removal of salmonella from the surface of smooth (honeydew) or complex (cantaloupe) melon rinds. melon rinds were spot-inoculated onto a 2.5 cm2 area of rind (squares) with approximately 6.0 log(10) cfu/square of an avirulent nalidixic acid-resistant strain of salmonella typhimurium. melons were washed by immersion in 1500 ml of water or 200 ppm total chlorine and allowed to soak or were scrubbed over the entire melon surf ...200515718029
3-methylthiopropionic acid ethyl ester, isolated from katsura-uri (japanese pickling melon, cucumis melo var. conomon), enhanced differentiation in human colon cancer cells.the fully ripened fruit of katsura-uri japanese pickling melon ( cucumis melo var. conomon) has rarely been used for food because the midripened fruit is utilized for making pickles, but the fully ripened fruit is no longer valuable for pickles due to the fruit body being too soft. we have considered the utilization of the fully ripened katsura-uri fruit that may be used for nonpickling products, particularly if the fully ripened fruit demonstrated health benefits such as anticarcinogenic proper ...200818426216
effect of hydrogen peroxide treatment on microbial quality and appearance of whole and fresh-cut melons contaminated with salmonella spp.the efficacy of hydrogen peroxide treatment on the inactivation of salmonella spp. inoculated on the external surface of cantaloupe and honeydew melon was investigated. salmonella was inoculated onto whole cantaloupe and honeydew melon to a final concentration of 4.65 log(10) cfu/cm(2) and 3.13 log(10) cfu/g, respectively. inoculated whole melons stored at 5 degrees c for up to 7 days were washed with water, 2.5% and 5% hydrogen peroxide at day 0 and 5. hydrogen peroxide (2.5% and 5%) treatments ...200415282126
reduction by gaseous ozone of salmonella and microbial flora associated with fresh-cut cantaloupe.this research investigates the efficacy of gaseous ozone, applied under partial vacuum in a controlled reaction chamber, for the elimination of salmonella inoculated on melon rind. the performance of high dose, short duration treatment with gaseous ozone, in this pilot system, on the microbial and sensory quality of fresh-cut cantaloupes was also evaluated. gaseous ozone (10,000 ppm for 30 min under vacuum) reduced viable, recoverable salmonella from inoculated physiologically mature non-ripe an ...200818456110
reduction of diabetes-induced renal oxidative stress by a cantaloupe melon extract/gliadin biopolymers, oxykine, in mice.oxidative stress is implicated as an important mechanism by which diabetes causes nephropathy. oxykine is the cantaloupe melon extract rich in vegetal superoxide dismutase covered by polymeric films of wheat matrix gliadin. in this study, we examined whether chronic oral administration of oxykine could prevent the progression of diabetic nephropathy induced by oxidative stress using preclinical rodent model of type 2 diabetes. we used female db/db mice and their non-diabetic db/m littermates. th ...200516179750
a multi-state outbreak of salmonella saintpaul in australia associated with cantaloupe consumption.a multi-state outbreak of salmonella enterica serovar saintpaul infection occurred in australia during october 2006. a case-control study conducted in three affected jurisdictions, new south wales, victoria and australian capital territory, included 36 cases with the outbreak-specific strain of s. saintpaul identified by multiple locus variable-number tandem repeat analysis (mlva) in a faecal specimen and 106 controls. consumption of cantaloupe (rockmelon) was strongly associated with illness (a ...200918559128
occurrence and distribution of ten viruses infecting cucurbit plants in guilan province, iran.during the 2006 and 2007 growing seasons, a systematic survey was conducted in open-field of melon (cucumis melo l.), cucumber (c. sativus l.), squash (cucurbita sp.), and watermelon (citrulus lanatus l.) crops in 16 major cucurbit-growing areas of guilan province in iran. symptomatic leaf samples were collected and screened by double-antibody sandwich elisa (das-elisa) or rt-pcr to detect zucchini yellow mosaic virus (zymv), watermelon mosaic virus (wmv), cucurbit aphid-borne yellows virus (cab ...200818564898
comparative histochemical analyses of oxidative burst and cell wall reinforcement in compatible and incompatible melon-powdery mildew (podosphaera fusca) interactions.the spatial-temporal expression patterns of oxidative burst and cell wall reinforcement were analyzed in leaves of resistant and susceptible melon (cucumis melo l.) cultivars in response to podosphaera fusca (fr.) braun & shishkoff, the main causal agent of powdery mildew in cucurbits. extensive development of powdery mildew mycelia and a progressive increase in haustorial count were recorded in the susceptible cultivar after 4d, while in the resistant cultivar powdery mildew failed to grow and ...200818585824
salmonella litchfield outbreak associated with a hotel restaurant--atlantic city, new jersey, 2007.on july 10, 2007, the pennsylvania department of health notified the new jersey department of health and senior services (njdhss) of three culture-confirmed cases of salmonella litchfield infection with matching pulsed-field gel electrophoresis (pfge) patterns. data from pulsenet, the national molecular subtyping network for foodborne disease surveillance, confirmed 11 cases (including the three from pennsylvania) of this rarely identified salmonella serotype in five states during a 5-week perio ...200818636066
on-farm and postharvest processing sources of bacterial contamination to melon rinds.multistate and international foodborne illness outbreaks, particularly involving cantaloupe and often involving rare salmonella spp., have increased dramatically over the past 13 years. this study assessed the sources and extent of melon rind contamination in production fields and at processing and packing facilities. in the spring of 1999, cantaloupe (cucumis melo l. [reticulatus group] cv. cruiser) sampled from two sites in the rio grande river valley showed that postharvest-processed melon ri ...200312540185
atp bioluminescence assay for estimation of microbial populations of fresh-cut melon.estimation of microbial numbers in foods by conventional microbiological techniques takes days, so there is a need for faster methods that can give results in minutes. research was undertaken to investigate the use of bioluminescent atp determination and a firefly luciferase assay to estimate the initial population of aerobic mesophilic bacteria on fresh-cut melons immediately after preparation and during storage at 5 or 15 degrees c for up to 12 days. populations of aerobic mesophilic bacteria ...200516300083
aflp analysis of a worldwide collection of didymella bryoniae.didymella bryoniae (anamorph phoma cucurbitacearum) is an ascomycete that causes gummy stem blight, a foliar disease that occurs on cucurbits in greenhouses and fields throughout the world. in a previous study using rapd analysis, little genetic diversity was found among isolates of d. bryoniae from new york and south carolina, usa. here we report the use of amplified fragment length polymorphism (aflp) analysis to assess the genetic variation within a worldwide collection of d. bryoniae, 102 fi ...200312825498
comparative morphology of eggs of heterophyids and clonorchis sinensis causing human infections in order to provide some clues for differential diagnosis of trematode infections in fecal examination, the comparative morphology of eggs of 5 kinds of heterophyid flukes (metagonimus yokogawai, heterophyes heterophyes nocens, heterophyopsis continua, stellantchasmus falcatus and pygidiopsis summa) and clonorchis sinensis was studied. the eggs were obtained from distal portion of uteri of worms which were recovered from men after treatment. the characteristic shape and apperance of each kind of ...198412891010
use of hydrogen peroxide in combination with nisin, sodium lactate and citric acid for reducing transfer of bacterial pathogens from whole melon surfaces to fresh-cut pieces.hydrogen peroxide (2.5%) alone or hydrogen peroxide (1%) in combination with nisin (25 microg/ml), sodium lactate (1%), and citric acid (0.5%) (hplnc) were investigated as potential sanitizers for reducing escherichia coli o157:h7 or listeria monocytogenes populations on whole cantaloupe and honeydew melons. whole cantaloupes inoculated with e. coli o157:h7 and l. monocytogenes at 5.27 and 4.07 log10 cfu/cm2, respectively, and whole honeydew melons inoculated with e. coli o157:h7 and l. monocyto ...200516043249
cerebroside elicitor confers resistance to fusarium disease in various plant species.abstract in the rice blast fungus pathosystem, cerebroside, a compound categorized as a sphingolipid, was found in our previous study to be a non-racespecific elicitor, which elicits defense responses in rice. here we describe that cerebroside c is produced in diverse strains of fusarium oxysporum, a common soilborne agent of wilt disease affecting a wide range of plant species. in addition, some type of cerebroside elicitor involving cerebroside a, b, or c was detected in other soilborne phytop ...200418943100
transformation of a muskmelon 'galia' hybrid parental line (cucumis melo l. var. reticulatus ser.) with an antisense acc oxidase gene.'galia' muskmelon (cucumis melo l. var. reticulatus ser.) has been recalcitrant to transformation by agrobacterium tumefaciens. transformation of the 'galia' male parental line, 'krymka', with an acc oxidase (cmaco-1) gene in antisense orientation is described herein. explants were transformed using a. tumefaciens strain abi, which contained a vector pcmaco1-as plasmid, bearing an antisense gene of cmaco-1 and the cp4 syn gene (glyphosate-tolerance). both cmaco-1 and cp4 syn genes were assessed ...200616362302
characterization of a regional population of fusarium oxysporum f. sp. niveum by race, cross pathogenicity, and vegetative compatibility.abstract eighty-eight isolates of fusarium oxysporum f. sp. niveum, collected from wilted watermelon plants and infested soil in maryland and dela-ware, were characterized by cross pathogenicity to muskmelon, race, and vegetative compatibility. four isolates (4.5%) were moderately pathogenic to >/=2 of 18 muskmelon cultivars in a greenhouse test, and one representative isolate also was slightly pathogenic in field microplots. the four isolates all were designated as race 2, and were in vegetativ ...200718943287
methylene blue staining method for identification of opisthorchis viverrini egg.methylene blue staining method was used to distinguish o. viverrini eggs from haplorchis taichui and prosthodendrium molenkampi eggs. all eggs were obtained from dissected adult worms, fixed in 10% formalin, and stained with methylene blue prior to light microscopy observation. the distinct musk-melon-like texture of the o. viverini eggshell surface and the thread-like texture of h. taichui eggshell surface were recognized, while p. molenkampi eggs showed a smooth eggshell. we also evaluated the ...200516438191
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