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rapid method for total carotenoid detection in canary yellow-fleshed watermelon.yellow-fleshed watermelons (citrullus lanatus[thunb.] matsum. and nakai) contain many different carotenoids, all in low to trace amounts. since there is not 1 predominant carotenoid in yellow-fleshed watermelon, testing the total carotenoid content among watermelon lines is important in determining the antioxidant potential and thus potential health benefits of different varieties. unfortunately, current methods to assay total carotenoid content are time consuming and require organic solvents. t ...200717995749
the expression of the saccharomyces cerevisiae hal1 gene increases salt tolerance in transgenic watermelon [citrullus lanatus (thunb.) matsun. & nakai.].an optimised agrobacterium-mediated gene transfer protocol was developed in order to obtain watermelon transgenic plants [citrullus lanatus (thunb.) matsun. & nakai.]. transformation efficiencies ranged from 2.8% to 5.3%, depending on the cultivar. the method was applied to obtain genetically engineered watermelon plants expressing the saccharomyces cerevisiae hal1 gene related to salt tolerance. in order to enhance its constitutive expression in plants, the hal1 gene was cloned in a pbin19 plas ...200312783167
a genetic linkage map for watermelon derived from a testcross population: ( citrullus lanatus var. citroides x c. lanatus var. lanatus) x citrullus colocynthis.a genetic linkage map was constructed for watermelon using a testcross population [plant accession griffin 14113 ( citrullus lanatus var. citroides) x new hampshire midget (nhm; c. lanatus var. lanatus)] x u.s. plant introduction (pi) 386015 ( citrullus colocynthis). the map contains 141 randomly amplified polymorphic dna (rapd) markers produced by 78 primers, 27 inter-simple sequence repeat (issr) markers produced by 17 primers, and a sequence-characterized amplified region (scar) marker that w ...200212582504
crop pollination from native bees at risk from agricultural intensification.ecosystem services are critical to human survival; in selected cases, maintaining these services provides a powerful argument for conserving biodiversity. yet, the ecological and economic underpinnings of most services are poorly understood, impeding their conservation and management. for centuries, farmers have imported colonies of european honey bees (apis mellifera) to fields and orchards for pollination services. these colonies are becoming increasingly scarce, however, because of diseases, ...200212486221
resistance of polish lines and hybrids of watermelon [citrullus lanatus (thunb.) matsum et nakai] to fusarium oxysporum at the seedling stage.watermelon is a species cultivated in the hot climate or in the greenhouse. since recently it has also started to be grown in the open in the polish climate. this species is frequently at risk of fusarium oxysporum infection. between 1996 and 1997 ten inbred lines and nine hybrids of polish origin were tested for resistance to this pathogen. the test was conducted with the use of four isolates of f. oxysporum: three from polish infected plants (formae speciales not determined), while the fourth ...200212080172
regulation of oxylipin synthesis.two very common groups of oxylipins formed in plants involve the conversion of fatty acid hydroperoxides, such as hydroperoxy-octadecatrienoic acid, into further metabolites by allene oxide synthase and hydroperoxide lyase. both of these oxylipin branch pathways appear to be ubiquitous or nearly so in plants, but the relative activities of these two branches vary among plant species. in most plants examined, including arabidopsis, product formation from either of these pathways is minimal until ...200011171228
cloning and molecular analyses of a gibberellin 20-oxidase gene expressed specifically in developing seeds of watermelon.to understand the biosynthesis and functional role of gibberellins (gas) in developing seeds, we isolated cv20ox, a cdna clone from watermelon (citrullus lanatus) that shows significant amino acid homology with ga 20-oxidases. the complementary dna clone was expressed in escherichia coli as a fusion protein, which oxidized ga(12) at c-20 to the c(19) compound ga(9), a precursor of bioactive gas. rna-blot analysis showed that the cv20ox gene was expressed specifically in developing seeds. the gen ...199910517828
biochemical analysis of plant protection afforded by a nonpathogenic endophytic mutant of colletotrichum magnaa nonpathogenic mutant of colletotrichum magna (path-1) was previously shown to protect watermelon (citrullus lanatus) and cucumber (cucumis sativus) seedlings from anthracnose disease elicited by wild-type c. magna. disease protection was observed in stems of path-1-colonized cucurbits but not in cotyledons, indicating that path-1 conferred tissue-specific and/or localized protection. plant biochemical indicators of a localized and systemic (peroxidase, phenylalanine ammonia-lyase, lignin, and ...19999952476
studies on toxigenic fungi in roasted foodstuff (salted seed) and halotolerant activity of emodin-producing aspergillus wentii.commercial roasted salted peanuts (3% nacl), popcorn (1% nacl), summer-squash (9% nacl), sunflower (3% nacl) and wild-melon (3% nacl) seeds are polluted with fungi, mostly aspergillus flavus, a. niger, penicillium chrysogenum, p. corylophilum and rhizopus stolonifer. contamination of popcorn with the fungi is about 10 times higher than in the other foods. these fungi, common also on unsalted seeds, are significantly inhibited in seeds (30% moisture content) treated with 9-21% nacl. the halotoler ...19989821293
production of an anti-allergic triterpene bryonolic acid, by plant cell cultures.cell suspension cultures of luffa cylindrica, citrullus lanatus, and related cucurbitaceous plants accumulate large quantities of bryonolic acid (3 beta-hydroxy-d:c-friedoolean-8-en-29-oic acid) [1], an acidic, pentacyclic triterpene found exclusively in the roots of the intact plants. this compound could readily be isolated from cultured cells with chcl3 and purified simply by recrystallization. pharmacological tests using mice demonstrated that bryonolic acid or its derivative is active agains ...19938463793
watermelon chlorotic stunt virus (wmcsv): a serious disease threatening watermelon production in jordan.the incidence of watermelon chlorotic stunt disease and the molecular characterization of the jordanian isolate of watermelon chlorotic stunt virus (wmcsv-[jo]) are described in this study. symptomatic leaf samples obtained from watermelon (citrullus lanatus thunb.), melon (cucumis melo l.), squash (cucurbita pepo), cucumber (cucumis sativus l.), and bottle gourd (lagenaria siceraria) plants were tested for wmcsv-[jo] infection by pcr. the virus could be detected in 8 melon and 87 watermelon sam ...201121399920
genetic diversity in watermelon (citrullus lanatus) landraces from zimbabwe revealed by rapd and ssr markers.low polymorphism in cultivated watermelon has been reported in previous studies, based mainly on us plant introductions and watermelon cultivars, most of which were linked to breeding programmes associated with disease resistance. since germplasm sampled in a putative centre of origin in southern africa may harbour considerably higher variability, dna marker-based diversity was estimated among 81 seedlings from eight accessions of watermelon collected in zimbabwe; five accessions of cow-melons ( ...201020887600
characterisation and functional properties of watermelon (citrullus lanatus) seed proteins.people in developing countries depend largely on non-conventional protein sources to augment the availability of proteins in their diets. watermelon seed meal is reported to contain an adequate amount of nutritional proteins that could be extracted for use as nutritional ingredients in food products.201120824684
watermelon profilin: characterization of a major allergen as a model for plant-derived food profilins.plant profilins have been reported as minor allergens. they are a well-known pan-allergen family responsible for cross-reactivity between plant-derived foods and pollens. watermelon profilin has been reported to be a major allergen in watermelon (citrullus lanatus).the aim of this study was to characterize recombinant watermelon profilin, confirming its reactivity for diagnostic purposes and the development of immunotherapy.201020484919
host range and complete genome sequence of cucurbit chlorotic yellows virus, a new member of the genus crinivirus.cucurbit chlorotic yellows virus (ccyv) causes chlorotic yellows on cucumber (cucumis sativus) and melon (cucumis melo) and is transmitted by bemisia tabaci biotype b and q whiteflies. to characterize the host range of ccyv, 21 cucurbitaceous and 12 other plant species were inoculated using whitefly vectors. all tested cucumis spp. except cucumis anguria and cucumis zeyheri were systemically infected with ccyv, although infection rates varied among species. citrullus lanatus, cucurbita pepo, and ...201020465411
inoculation of plants with begomoviruses by particle bombardment without cloning: using rolling circle amplification of total dna from infected plants and whiteflies.a new system for inoculation of plants with begomoviral dna without cloning or the use insect vectors is described. total dna extracted from begomovirus-infected plants was amplified by rolling circle amplification (rca) using the bacteriophage phi29 dna polymerase, and inoculated to plants by particle bombardment. infection rates of up to 100% were obtained using this technique. this technique successfully inoculated all the begomoviruses evaluated: five bipartite (bean golden yellow mosaic vir ...201020447420
establishment of a transgenic hairy root system in wild and domesticated watermelon (citrullus lanatus) for studying root vigor under drought.root vigor is an important trait for the growth of terrestrial plants, especially in water-deficit environments. although deserts plants are known for their highly developed root architecture, the molecular mechanism responsible for this trait has not been determined. here we established an efficient protocol for the genetic manipulation of two varieties of watermelon plants: a desert-grown wild watermelon that shows vigorous root growth under drought, and a domesticated cultivar showing retarda ...201020445980
non-synonymous single nucleotide polymorphisms in the watermelon eif4e gene are closely associated with resistance to zucchini yellow mosaic virus.zucchini yellow mosaic virus (zymv) is one of the most economically important potyviruses infecting cucurbit crops worldwide. using a candidate gene approach, we cloned and sequenced eif4e and eif(iso)4e gene segments in watermelon. analysis of the nucleotide sequences between the zymv-resistant watermelon plant introduction pi 595203 (citrullus lanatus var. lanatus) and the zymv-susceptible watermelon cultivar 'new hampshire midget' ('nhm') showed the presence of single nucleotide polymorphisms ...200919820912
recovery from cucurbit leaf crumple virus (family geminiviridae, genus begomovirus) infection is an adaptive antiviral response associated with changes in viral small rnas.a strong recovery response occurs in cantaloupe (cucumis melo) and watermelon (citrullus lanatus) infected with the bipartite begomovirus cucurbit leaf crumple virus (culcrv). this response is characterized by initially severe symptoms, which gradually become attenuated (almost symptomless). an inverse relationship was detected between viral dna levels and recovery, indicating that recovered tissues had reduced viral titers. recovered tissues also were resistant to reinfection with culcrv; i.e., ...200818943741
response of seedlings of a dwarf and a normal strain of watermelon to gibberellins.hypocotyl and root elongation in a dwarf and a normal strain of watermelon (citrullus lanatus [thunb.] matsu.) in the absence or presence of different gibberellins was investigated in seedlings grown under gold fluorescent light or in darkness. the normal strain, "sugar baby," responded only slightly to the gibberellic acids employed. at appropriate concentrations all of the gibberellic acids were capable of normalizing growth in the monorecessive dwarf strain, wb-2, in darkness or in light. gib ...197416658700
qualitative inheritance of rind pattern and flesh color in watermelon.watermelon [citrullus lanatus (thunb.) matsum. & nakai var. lanatus] is a diverse species, with fruits of different sizes, shapes, rind patterns, and flesh colors. this study measured the inheritance of novel rind phenotypes and verified the genetics of white, red, salmon yellow, and canary yellow flesh colors. for each of the 11 crosses, six generations (p(a)s1, p(b)s1, f1, f2, bc1p(a), and bc1p(b)) were produced to form 11 families. three new genes were identified and designated as follows: sc ...200616489140
co-expression of cytochrome b561 and ascorbate oxidase in leaves of wild watermelon under drought and high light conditions.despite carrying out c3 photosynthesis, wild watermelon (citrullus lanatus sp.) exhibits exceedingly good tolerance to severe drought at high light intensities. however, the mechanism(s) by which this plant protects itself from photodamage has yet to be elucidated. in this study, we characterized wild watermelon cytochrome b561 (cyt b561), which potentially mediates regeneration of apoplastic ascorbate by transferring electrons from cytosolic ascorbate across the plasma membrane. two cdna specie ...200516020428
watermelon (citrullus lanatus) hydroperoxide lyase greatly increases c6 aldehyde formation in transgenic leaves.fatty acid hydroperoxide lyase (hl) is the key enzyme for the production of the "green note"compounds, leaf aldehyde [(2e)-hexenal] and leaf alcohol [(3z)-hexenol], in plant tissues. a cdna encoding hl was cloned from leaves of watermelon (citrullus lanatus) and expressed in nicotiana tabacum. the enzyme is 3 times more active with 13-hydroperoxylinolenic acid than with 13-hydroperoxylinoleic acid. the activity against 9-hydroperoxides of polyunsaturated fatty acids is minimal. enzyme activity o ...200515769134
inheritance of resistance to zucchini yellow mosaic virus and watermelon mosaic virus in watermelon.high resistance to zucchini yellow mosaic virus-china strain (zymv-ch) and moderate resistance to watermelon mosaic virus (wmv) were found in a selection of pi 595203 (citrullus lanatus var. lanatus), an egusi type originally collected in nigeria. mixed inoculations showed primarily that these two viruses have no cross-protection. this fact may explain the high frequency of mixed infection often observed in commercial fields. when plants were inoculated with a mixture of the two viruses, the fre ...200415475395
plant er genes that encode photorespiratory enzymes confer resistance against disease.downy mildew caused by the oomycete pathogen pseudoperonospora cubensis is a devastating foliar disease of cucurbits worldwide. we previously demonstrated that the wild melon line pi 124111f (pi) is highly resistant to all pathotypes of p. cubensis. that resistance was controlled genetically by two partially dominant, complementary loci. here, we show that unlike other plant disease resistance genes, which confer an ability to resist infection by pathogens expressing corresponding avirulence gen ...200414688292
influence of temperature on biomass, iron metabolism and some related bioindicators in tomato and watermelon plants.tomato, lycopersicon esculentum l. cv. rx-335, and watermelon plants, citrullus lanatus [thom.] mansf. cv. f-90 were grown under controlled conditions at three different temperatures (10 degrees, 25 degrees and 35 degrees c) for 30 days. the aim of the experiment was to analyse the effect of the different temperatures on fe uptake and distribution, as well as the behaviour of the main bioindicators of this element. thus, we analysed the total and free fe concentrations and h2o2 concentrations, a ...200314593808
protective effect of diyarbak-¦r watermelon juice on carbon tetrachloride-induced toxicity in rats.the present study was designed to evaluate the effect of diyarbak-¦r watermelon (citrullus lanatus cv. s++rme) juice on lipid peroxidation states in rat liver, kidney and brain. in vivo administration of carbon tetrachloride (ccl(4)) once a week for 28days caused a significant elevation of serum markers of liver damage, aspartate aminotransferase (ast), alanine aminotransferase (alt), total bilirubin (tb) and decrease in albumin when compared to the control group. however, administration of carb ...201121740951
redox signaling and cbf-responsive pathway are involved in salicylic acid-improved photosynthesis and growth under chilling stress in watermelon.salicylic acid (sa) plays an important role in plant response to abiotic stresses. this study investigated the potential role of sa in alleviating the adverse effects of chilling stress on photosynthesis and growth in watermelon (citrullus lanatus). chilling stress induced the simultaneous accumulation of free and conjugated sa in watermelon plants, and the chilling-induced sa production was attributed to the phenylalanine ammonia-lyase pathway. applying sa at moderate concentrations induced chi ...201627777580
assessment of dapg-producing pseudomonas fluorescens for management of meloidogyne incognita and fusarium oxysporum on watermelon.pseudomonas fluorescens isolates clinto 1r, wayne 1r, and wood 1r, which produce the antibiotic 2,4-diacetylphloroglucinol (dapg), can suppress soilborne diseases and promote plant growth. consequently, these beneficial bacterial isolates were tested on watermelon plants for suppression of meloidogyne incognita (root-knot nematode: rkn) and fusarium oxysporum f. sp. niveum (fon). in a greenhouse trial, wayne 1r root dip suppressed numbers of rkn eggs per gram root on 'charleston gray' watermelon ...201627168652
genetic transformation and plant regeneration of watermelon using agrobacterium tumefaciens.adventitious shoots formed on the proximal cut edges of different cotyledonary explants of watermelon [citrullus lanatus (thunb.) matsum. & nakai; cvs. sweet gem and gold medal] cultured on murashige and skoog's (ms) medium with 1 mgl(-1) 6-benzyladenine (ba). light (16-h photoperiod, about 7 wm(-2) cool-white fluorescent lamps) was essential for shoot formation. to obtain transformed plants, cotyledonary explants of 'sweet gem' were cocultured with agrobacterium tumefaciens lba4404, a disarmed ...199424193834
antifungal effect of eugenol and carvacrol against foodborne pathogens aspergillus carbonarius and penicillium roqueforti in improving safety of fresh-cut watermelon.essential oil components eugenol and carvacrol (ranging between 100 and 200 ppm for carvacrol and between 250 and 750 ppm for eugenol) were tested for antifungal activity against foodborne pathogenic fungal species aspergillus carbonarius a1102 and penicillium roqueforti ptfkk29 in in vitro and in situ conditions.201726401354
antiviral activity of thuja orientalis extracts against watermelon mosaic virus (wmv) on citrullus lanatus.watermelon mosaic potyvirus (wmv) is considered as an important virus infecting watermelon and causing adverse effects on crop productivity. to overcome this problem one of the main objectives of plant breeders is to make these strains less effective in the ability to infect plants by treatment with plant extracts. due to the advantages of plant tissue culture, in vitro, in the process of the selection of different cultivars under biotic stress, this study was conducted to achieve this aim by ev ...201525737655
a gbs-snp-based linkage map and quantitative trait loci (qtl) associated with resistance to fusarium oxysporum f. sp. niveum race 2 identified in citrullus lanatus var. citroides.a major qtl for resistance to fusarium oxysporum f. sp. niveum race 2 was mapped to a narrow 1.2 mb interval using a high-density gbs-snp linkage map, the first map of citrullus lanatus var. citroides. fusarium wilt, a fungal disease caused by fusarium oxysporum f. sp. niveum (fon), devastates watermelon crop production worldwide. several races, which are differentiated by host range, of the pathogen exist. resistance to fon race 2, a particularly virulent strain prevalent in the united states, ...201727803951
ccr4-not complex subunit not2 plays critical roles in vegetative growth, conidiation and virulence in watermelon fusarium wilt pathogen fusarium oxysporum f. sp. niveum.ccr4-not complex is a multifunctional regulator that plays important roles in multiple cellular processes in eukaryotes. in the present study, the biological function of fonnot2, a core subunit of the ccr4-not complex, was explored in fusarium oxysporum f. sp. niveum (fon), the causal agent of watermelon wilt disease. fonnot2 was expressed at higher levels in conidia and germinating conidia and during infection in fon-inoculated watermelon roots than in mycelia. targeted disruption of fonnot2 re ...201627695445
the fonsix6 gene acts as an avirulence effector in the fusarium oxysporum f. sp. niveum - watermelon pathosystem.when infecting a host plant, the fungus fusarium oxysporum secretes several effector proteins into the xylem tissue to promote virulence. however, in a host plant with an innate immune system involving analogous resistance proteins, the fungus effector proteins may trigger resistance, rather than promoting virulence. identity of the effector genes of fusarium oxysporum f. sp. niveum (fon) races that affect watermelon (citrullus lanatus) are currently unknown. in this study, the six6 (secreted in ...201627320044
the components of rice and watermelon root exudates and their effects on pathogenic fungus and watermelon defense.watermelon (citrullus lanatus) is susceptible to wilt disease caused by the fungus fusarium oxysporum f. sp niveum (fon). intercropping management of watermelon/aerobic rice (oryza sativa) alleviates watermelon wilt disease, because some unidentified component(s) in rice root exudates suppress fon sporulation and spore germination. here, we show that the phenolic acid p-coumaric acid is present in rice root exudates only, and it inhibits fon spore germination and sporulation. we found that exoge ...201627217091
fruit quality of seedless watermelon grafted onto squash rootstocks under different production systems.the market demand for seedless watermelon has been continuously increasing because of consumer preference. grafting is a useful tool to manage soil-borne diseases in watermelon production, but the use of squash rootstocks may negatively affect watermelon fruit quality. currently, most research has focused on seeded cultivars while grafting effects on seedless watermelons remain largely unknown. this multi-season study was conducted to assess the effects of squash rootstocks, including both cucur ...201728369915
comparative analysis of type iii secreted effector genes reflects divergence of acidovorax citrulli strains into three distinct lineages.acidovorax citrulli causes bacterial fruit blotch of cucurbits, a serious economic threat to watermelon (citrullus lanatus) and melon (cucumis melo) production worldwide. based on genetic and biochemical traits, a. citrulli strains have been divided into two distinct groups: group i strains have been mainly isolated from various non-watermelon hosts, while group ii strains have been generally isolated from and are highly virulent on watermelon. the pathogen depends on a functional type iii secre ...201424848275
resistance to southern root-knot nematode (meloidogyne incognita) in wild watermelon (citrullus lanatus var. citroides).southern root-knot nematode (rkn, meloidogyne incognita) is a serious pest of cultivated watermelon (citrullus lanatus var. lanatus) in southern regions of the united states and no resistance is known to exist in commercial watermelon cultivars. wild watermelon relatives (citrullus lanatus var. citroides) have been shown in greenhouse studies to possess varying degrees of resistance to rkn species. experiments were conducted over 2 yr to assess resistance of southern rkn in c. lanatus var. citro ...201627168648
penetration, post-penetration development, and reproduction of meloidogyne incognita on cucumis melo var. texanus.cucumis melo var. texanus, a wild melon commonly found in the southern united states and two accessions, burleson co. and mx 1230, expressed resistance to meloidogyne incognita in preliminary experiments. to characterize the mechanism of resistance, we evaluated root penetration, post-penetration development, reproduction, and emigration of m. incognita on these two accessions of c. melo var. texanus. additionally, we evaluated 22 accessions of c. melo var. texanus for their reaction against m. ...201323589661
predicting damage of meloidogyne incognita on watermelon.quantitative growth response of watermelon (citrullus lanatus) sensitive to meloidogyne incognita is poorly understood. determination of soil population densities of second-stage juveniles (j2) of m. incognita with baermann funnel extraction often is inaccurate at low soil temperatures. in greenhouse experiments, three sandy soils were inoculated with dilution series of population densities of eggs or j2 of m. incognita and planted in small containers to watermelon 'royal sweet' or subjected to ...201223482631
determination of free amino acids in african gourd seed milks by capillary electrophoresis with light-emitting diode induced fluorescence and laser-induced fluorescence detection.a ce technique coupled to lif detection (488 nm) or led-induced fluorescence detection (470 nm) has been evaluated to acquire a cheap way to analyze amino acids (aas) whilst maintaining the best sensitivity. to quantitate aas in milk of cucurbitaceae of sub-saharan africa, they were labeled with fitc. we used an optimized separation buffer composed of 30 mm boric acid buffer adjusted to ph 9.3 with naoh (1 m) containing 12 mm sds and 5% ethylene glycol v/v; prior to the injections, the derivatiz ...201323857426
reaction of ten cultivars of watermelon (citrullus lanatus) to a puerto rican population of meloidogyne incognita.ten cultivars of watermelon were evaluated for their response to a puerto rican population of meloidogyne incognita under greenhouse conditions in a 2-year study (1989 and 1990). ten-day-old seedlings were planted in steam-sterilized soil in 15-cm-d plastic pots. the nematode inoculum consisted of 10,000 eggs and (or) second-stage juveniles (j2)/plant. the cultivars were sugar baby, charleston gray, seedless, prince charles, charleston 76, jubilee, florida giant, royal charleston, royal sweet, a ...199419279940
genetic analysis and chromosome mapping of resistance to fusarium oxysporum f. sp. niveum (fon) race 1 and race 2 in watermelon (citrullus lanatus l.).fusarium wilt (fw) caused by fusarium oxysporum f. sp. niveum (fon) is the major soilborne disease of watermelon (citrullus lanatus l.). the development and deployment of resistant cultivars is generally considered to be an effective approach to control fw. in this study, an f8 population consisting of 103 recombinant inbred lines derived from a cross between the cultivar 97103 and a wild accession pi 296341-fr was used for fon race 1 and race 2 fungal inoculations. one major qtl on chromosome 1 ...201726347205
construction of cdna expression library of watermelon for isolation of clwrky1 transcription factors gene involved in resistance to fusarium wilt.full-length cdnas are very important for genome annotation and functional analysis of genes. the number of full-length cdnas from watermelon remains limited. here we report first the construction of a full-length enriched cdna library from fusarium wilt stressed watermelon (citrullus lanatus thunb.) cultivar pi296341 root tissues using the smart method. the titer of primary cdna library and amplified library was 2.21 x 10(6) and 2.0 x 10(10) pfu/ml, respectively and the rate of recombinant was a ...201425296501
microplot evaluation of rootstocks for control of meloidogyne incognita on grafted tomato, muskmelon, and watermelon.microplot experiments were conducted over two years (four growing seasons) to evaluate meloidogyne incognita resistance in rootstocks used for grafted tomato (solanum lycopersicum), muskmelon (cucumis melo), and watermelon (citrullus lanatus). three tomato rootstocks; 'tx301', 'multifort', and 'aloha', were tested in addition to the nongrafted scion, 'florida-47'. two muskmelon rootstocks; cucumis metuliferus and 'tetsukabuto' (cucurbita maxima × cucurbita moschata) were evaluated with the nongr ...201123431109
squash leaf curl virus (slcv): a serious disease threatening cucurbits production in palestine.the incidence of squash leaf curl disease and molecular characterization of the palestinian isolate of squash leaf curl virus [slcv-(pal)] are described in this study. symptomatic leaf samples obtained from squash (cucurbita pepo), watermelon [citrullus lanatus (thunb.)], and cucumber (cucumis sativus l.) plants were tested for slcv-[pal] infection by pcr and rca. slcv was also found to occur naturally in chenopodium murale, convolvulus sp, and prosporis farcta which showed yellowing. the diseas ...201424258392
transmission of squash vein yellowing virus to and from cucurbit weeds and effects on sweetpotato whitefly (hemiptera: aleyrodidae) behavior.since 2003, growers of florida watermelon [citrullus lanatus (thunb.) matsum. and nakai] have periodically suffered large losses from a disease caused by squash vein yellowing virus (sqvyv), which is transmitted by the whitefly middle east-asia minor 1 (meam1), formerly bemisia tabaci (gennadius) biotype b. common cucurbit weeds like balsam apple (momordica charantia l.) and smellmelon [cucumis melo var. dudaim (l.) naud.] are natural hosts of sqvyv, and creeping cucumber (melothria pendula l.) ...201627400705
the use of latent class analysis to estimate the sensitivities and specificities of diagnostic tests for squash vein yellowing virus in cucurbit species when there is no gold standard.squash vein yellowing virus (sqvyv) is the causal agent of viral watermelon vine decline, one of the most serious diseases in watermelon (citrullus lanatus l.) production in the southeastern united states. at present, there is not a gold standard diagnostic test for determining the true status of sqvyv infection in plants. current diagnostic methods for identification of sqvyv-infected plants or tissues are based on the reverse-transcription polymerase chain reaction (rt-pcr), tissue blot nuclei ...201323883156
high-level expression of a novel chromoplast phosphate transporter clpht4;2 is required for flesh color development in watermelon.chromoplast development plays a crucial role in controlling carotenoid content in watermelon flesh. modern cultivated watermelons with colorful flesh are believed to originate from pale-colored and no-sweet progenitors. but the molecular basis of flesh color formation and regulation is poorly understood. more chromoplasts and released carotenoid globules were observed in the red-fleshed fruit of the 97103 cultivar than in the pale-colored fruits of the pi296341-fr line. transcriptome profiles of ...201727787901
recombinant yeast as a functional tool for understanding bitterness and cucurbitacin biosynthesis in watermelon (citrullus spp.).cucurbitacins are a group of bitter-tasting oxygenated tetracyclic triterpenes that are produced in the family cucurbitaceae and other plant families. the natural roles of cucurbitacins in plants are probably related to defence against pathogens and pests. cucurbitadienol, a triterpene synthesized from oxidosqualene, is the first committed precursor to cucurbitacins produced by a specialized oxidosqualene cyclase termed cucurbitadienol synthase. we explored cucurbitacin accumulation in watermelo ...201525308777
a new cryptic virus belonging to the family partitiviridae was found in watermelon co-infected with melon necrotic spot virus.a novel virus was detected in watermelon plants (citrullus lanatus thunb.) infected with melon necrotic spot virus (mnsv) using solid next-generation sequence analysis. in addition to the expected msnv genome, two double-stranded rna (dsrna) segments of 1,312 and 1,118 bp were also identified and sequenced from the purified virus preparations. these two dsrna segments encode two putative partitivirus-related proteins, an rna-dependent rna polymerase (rdrp) and a capsid protein, which were sequen ...201323775759
evaluation of watermelon germplasm for resistance to phytophthora blight caused by phytophthora capsici.this study was conducted to determine the phytophthora rot resistance of 514 accessions of watermelon germplasm, citrullus lanatus var lanatus. about 46% of the 514 accessions tested were collections from uzbekistan, turkey, china, u.s.a., and ukraine. phytophthora capsici was inoculated to 45-day-old watermelon seedlings by drenching with 5 ml of sporangial suspension (10(6) sporangia/ml). at 7 days after inoculation, 21 accessions showed no disease symptoms while 291 accessions of susceptible ...201325288932
molecular characterization of watermelon chlorotic stunt virus (wmcsv) from palestine.the incidence of watermelon chlorotic stunt disease and molecular characterization of the palestinian isolate of watermelon chlorotic stunt virus (wmcsv-[pal]) are described in this study. symptomatic leaf samples obtained from watermelon citrullus lanatus (thunb.), and cucumber (cucumis sativus l.) plants were tested for wmcsv-[pal] infection by polymerase chain reaction (pcr) and rolling circle amplification (rca). disease incidence ranged between 25%-98% in watermelon fields in the studied ar ...201424956181
characterization, expression patterns and functional analysis of the mapk and mapkk genes in watermelon (citrullus lanatus).mitogen-activated protein kinase (mapk) cascades, which consist of three functionally associated protein kinases, namely mekks, mkks and mpks, are universal signaling modules in all eukaryotes and have been shown to play critical roles in many physiological and biochemical processes in plants. however, little or nothing is known about the mpk and mkk families in watermelon.201526700161
fractionation, amino acid profiles, antimicrobial and free radical scavenging activities of citrullus lanatus seed protein.in the present study, a modified osborne fractionation method was followed to isolate albumin (calb), globulin (cglo), prolamin (cpro) and glutelin (cglu) successively from seeds of citrullus lanatus (watermelon). this research work was undertaken to investigate the antimicrobial and antioxidant activities of isolated protein fractions of c. lanatus seed. amino acid composition and molecular weight distribution were determined to establish their relationship with antimicrobial and antioxidant ac ...201728393615
caulobacter flavus sp. nov., a stalked bacterium isolated from rhizosphere soil.a gram-stain-negative, aerobic, yellow-pigmented and rod-shaped bacterium with a single polar flagellum or a stalk, designated strain rhgg3t, was isolated from rhizosphere soil of cultivated watermelon (citrullus lanatus) collected from hefei, china. optimal growth of strain rhgg3t was observed at ph 7.0 and 28-30 °c. cells were catalase-positive and oxidase-negative. phylogenetic analysis based on 16s rrna gene sequences indicated that strain rhgg3t belonged to the genus caulobacter and showed ...201526354335
standardized sampling plan for the thrips frankliniella schultzei (thysanoptera: thripidae) on watermelon crops.sampling plans are essential components of integrated pest management programs. the thrips frankliniella schultzei (trybom) (thysanoptera: thripidae) is an important pest of watermelon crops. despite the importance of sampling plans and of f. schultzei as a pest of watermelon crops, no research has been previously conducted on this subject for this crop. the objective of this work was to create a standardized sampling plan for f. schultzei in watermelon crops. over two consecutive years, weekly ...201728334069
in vitro physicochemical, phytochemical and functional properties of fiber rich fractions derived from by-products of six fruits.a comparative study was done on the health promoting and functional properties of the fibers obtained as by-products from six fruits viz., pomace of carambola (averrhoa carambola l.) and pineapple (ananas comosus l. merr), peels of watermelon (citrullus lanatus), burmese grape (baccurea sapida muell. arg) and khasi mandarin orange (citrus reticulata blanco), and blossom of seeded banana (musa balbisiana, abb). highest yield of fiber was obtained from burmese grape peel (bgpl, 79.94 ± 0.41 g/100  ...201627570274
draft genome sequence of bitter gourd (momordica charantia), a vegetable and medicinal plant in tropical and subtropical regions.bitter gourd (momordica charantia) is an important vegetable and medicinal plant in tropical and subtropical regions globally. in this study, the draft genome sequence of a monoecious bitter gourd inbred line, ohb3-1, was analyzed. through illumina sequencing and de novo assembly, scaffolds of 285.5 mb in length were generated, corresponding to ∼84% of the estimated genome size of bitter gourd (339 mb). in this draft genome sequence, 45,859 protein-coding gene loci were identified, and transposa ...201728028039
exposure effects on the productivity of commercial bombus impatiens (hymenoptera: apidae) quads during bloom in watermelon fields.in light of population declines of honey bees (apis mellifera l.), research has refocused attention on alternative pollinators and their potential to fulfill pollination services within economically important agricultural crops. bumble bees are one such alternative, and within the past 20 yr, these pollinators have been reared and sold as commercial pollinators. investigation into their use has been limited and more research is needed to improve pollinator effectiveness in field settings. quad p ...201526470323
economic injury levels and sequential sampling plans for frankliniella schultzei in watermelon crops.the thrips frankliniella schultzei is an important watermelon pest. nevertheless, economic injury levels and sampling plans for this pest have not yet been determined for this crop. thus, the objective of the present study was to determine the economic injury levels and develop sequential sampling plans for f. schultzei in conditions of low, medium and high fruit prices.201627860205
in vitro assessment of physiological changes of watermelon (citrullus lanatus) upon iron oxide nanoparticles exposure.with the rapid development of nanotechnology, developing nano iron fertilizer is an important strategy to alleviate fe deficiency and elevate fe fertilization effect in agricultural applications. in this study, watermelon seedlings were grown in soil amended with iron oxide nanoparticles (γ-fe2o3 nps) at different concentrations (0, 20, 50, 100 mg/l). the content of soluble sugar and protein, content of chlorophyll and malondialdehyde (mda), and activity of antioxidant enzymes of watermelon leav ...201627518375
a casparian strip domain-like gene, caspl, negatively alters growth and cold tolerance.a cold-induced transcript encoding a casparian strip membrane domain (casp)-like protein (clcaspl) was identified in watermelon (citrullus lanatus). fluorescence microscopy analysis showed that clcaspl-gfp is localized in the plasma membrane. the orthologous gene in arabidopsis thaliana (atcaspl4c1) was also found to play an important role in cold tolerance. expression analysis using a β-glucuronidase (gus) reporter reveals that atcaspl4c1 is widely expressed in a variety of organs and is cold i ...201526399665
association between photosynthesis and contrasting features of minor veins in leaves of summer annuals loading phloem via symplastic versus apoplastic routes.foliar vascular anatomy and photosynthesis were evaluated for a number of summer annual species that either load sugars into the phloem via a symplastic route (cucumis sativus l. cv. straight eight; cucurbita pepo l. cv. italian zucchini romanesco; citrullus lanatus l. cv. faerie hybrid; cucurbita pepo l. cv. autumn gold) or an apoplastic route (nicotiana tabacum l.; solanum lycopersicum l. cv. brandywine; gossypium hirsutum l.; helianthus annuus l. cv. soraya), as well as winter annual apoplast ...201424450755
genome-wide identification and expression analysis of cllax, clpin and clabcb genes families in citrullus lanatus under various abiotic stresses and grafting.auxin plays an important role in regulating plant growth and development as well as in the response of plants to abiotic stresses. auxin is transported by three kinds of major protein families, including the auxin resistant 1/like aux1 (aux⁄lax) influx carriers, the pin-formed (pin) efflux carriers and the atp binding cassette b/p-glycoprotein/multidrug-resistance (abcb/mdr/pgp) efflux/condition carriers. the biological function of several auxin transporter genes has been well characterized in a ...201728388893
a comparative study on the effect of conventional thermal pasteurisation, microwave and ultrasound treatments on the antioxidant activity of five fruit juices.a comparative study on the effect of conventional thermal pasteurisation, microwave and ultrasound treatments on the phytochemical and antioxidant activities of juices from carambola (averrhoa carambola l.), black jamun (syzygium cumuni l.skeels.), watermelon (citrullus lanatus var lanatus), pineapple (ananas comosus l. merr) and litchi (litchi chinensis sonn.) was carried out. depending on the type of fruit sample and treatment, increase or decrease in phytochemical values was observed. overall ...201626190045
global expressions landscape of nac transcription factor family and their responses to abiotic stresses in citrullus lanatus.watermelon (citrullus lanatus) is one xerophyte that has relative higher tolerance to drought and salt stresses as well as more sensitivity to cold stress, compared with most model plants. these characteristics facilitate it a potential model crop for researches on salt, drought or cold tolerance. in this study, a genome-wide comprehensive analysis of the clnac transcription factor (tf) family was carried out for the first time, to investigate their transcriptional profiles and potential functio ...201627491393
linking pollination effectiveness and interspecific displacement success in bees.pollen deposition, a surrogate for bee efficiency, becomes increasingly important during their interspecific interactions. we conducted field experiments on highly cross-pollinated melon (cucumis melo) and watermelon (citrullus lanatus) in order to understand how bee species with different pollination efficiencies displace each other from floral resources. we observed significant displacement of less abundant but more efficient bees by the more abundant but less efficient bees in both crops, whi ...201526013126
oil and fatty acid contents in seed of citrullus lanatus schrad.intact seed of 475 genebank accessions of citrullus ( c. lanatus var. lanatus and c. lanatus var. citroides) were analyzed for percent oil content using td-nmr. extracts from whole seed of 96 accessions of c. lanatus (30 var. citroides, 33 var. lanatus, and 33 egusi), c. colocynthis (n = 3), c. ecirrhosus (n = 1), c. rehmii (n = 1), and benincasa fistulosa (n = 3) were also analyzed for their fatty acids content. among the materials analyzed, seed oil content varied from 14.8 to 43.5%. mean seed ...201222540530
expression in arabidopsis of a nucellus-specific promoter from watermelon (citrullus lanatus).though many tissue-specific promoters have been identified, few have been associated specifically with the angiospermous megasporangium (nucellus). in the present study the 2000-bp regulatory region upstream to the watermelon, citrullus lanatus (thunb.) matsum & nakai, gene wm403 (genbank accession no. af008925), which shows nucellus-specific expression, was cloned from watermelon gdna and fused to the β-glucuronidase reporter gene (gus). the resulting plasmid, wm403 prom::gus(+), which also con ...201021802614
understanding invasion history and predicting invasive niches using genetic sequencing technology in australia: case studies from cucurbitaceae and boraginaceae.part of the challenge in dealing with invasive plant species is that they seldom represent a uniform, static entity. often, an accurate understanding of the history of plant introduction and knowledge of the real levels of genetic diversity present in species and populations of importance is lacking. currently, the role of genetic diversity in promoting the successful establishment of invasive plants is not well defined. genetic profiling of invasive plants should enhance our understanding of th ...201627766152
cloning and expression analysis of the ccrboh gene encoding respiratory burst oxidase in citrullus colocynthis and grafting onto citrullus lanatus (watermelon).a full-length drought-responsive gene ccrboh, encoding the respiratory burst oxidase homologue (rboh), was cloned in citrullus colocynthis, a very drought-tolerant cucurbit species. the robh protein, also named nadph oxidase, is conserved in plants and animals, and functions in the production of reactive oxygen species (ros). the ccrboh gene accumulated in a tissue-specific pattern when c. colocynthis was treated with peg, abscisic acid (aba), salicylic acid (sa), jasmonic acid (ja), or nacl, wh ...201020181664
acc synthase genes are polymorphic in watermelon (citrullus spp.) and differentially expressed in flowers and in response to auxin and gibberellin.the flowering pattern of watermelon species (citrullus spp.) is either monoecious or andromonoecious. ethylene is known to play a critical role in floral sex determination of cucurbit species. in contrast to its feminizing effect in cucumber and melon, in watermelon ethylene promotes male flower development. in cucumber, the rate-limiting enzyme of ethylene biosynthesis, 1-aminocyclopropane-1-carboxylate (acc) synthase (acs), regulates unisexual flower development. to investigate the role of eth ...200818367517
the cucurbits of mediterranean antiquity: identification of taxa from ancient images and descriptions.a critical analysis was made of cucurbit descriptions in dioscorides' de materia medica, columella's de re rustica and pliny's historia naturalis, works on medicine, agriculture and natural science of the 1st century ce, as well as the mishna and tosefta, compilations of rabbinic law derived from the same time period together with cucurbit images dating from antiquity including paintings, mosaics and sculpture. the goal was to identify taxonomically the mediterranean cucurbits at the time of the ...200717932073
simple sequence repeats in watermelon (citrullus lanatus (thunb.) matsum. & nakai).simple sequence repeat length polymorphisms were utilized to examine genetic relatedness among accessions of watermelon (citrullus lanatus (thunb.) matsum. & nakai). a size-fractionated taqi genomic library was screened for the occurrence of dimer and trimer simple sequence repeats (ssrs). a total of 96 (0.53%) ssr-bearing clones were identified and the inserts from 50 of these were sequenced. the dinucleotide repeats (ct)n and (ga)n accounted for 82% of the ssrs sequenced. pcr primer pairs flan ...19979276932
quantification of lycopene, β-carotene, and total soluble solids in intact red-flesh watermelon (citrullus lanatus) using on-line near-infrared spectroscopy.a great interest has recently been focused on lycopene and β-carotene, because of their antioxidant action in the organism. red-flesh watermelon is one of the main sources of lycopene as the most abundant carotenoid. the use of near-infrared spectroscopy (nirs) in post-harvesting has permitted us to rapidly quantify lycopene, β-carotene, and total soluble solids (tss) on single intact fruits. watermelons, harvested in 2013-2015, were submitted to near-infrared (nir) radiation while being transpo ...201728398228
host use of bactrocera latifrons, a new invasive tephritid species in tanzania.bactrocera latifrons (hendel) is a pest of asian origin, first detected in africa in 2006. we assessed the host utilization of this quarantine pest in morogoro region, eastern central tanzania, by collecting a wide range of cultivated and wild host plants of species belonging to solanaceae and cucurbitaceae from april 2007 to april 2008. fruit were collected from 29 plant species and varieties (16 solanaceae and 13 cucurbitaceae) in all agroecological zones of morogoro region. twelve solanaceous ...201020214370
taxonomic significance of trichomes micromorphology in cucurbits.studies on trichomes micromorphology using scanning electron microscope (sem) were undertaken in 23 species with one variety under 13 genera of the family cucurbitaceae (viz., benincasa hispida (thunb.) cogn., citrullus lanatus (thunb.) matsum. & nakai, cucumis melo var. agrestis naudin, cucumis sativus l., diplocyclos palmatus (l.) c. jeffrey, edgaria dargeelingensis c.b. clarke, gynostemma burmanicum king ex chakr., gynostemma pentaphyllum (thunb.) makino, gynostemma pubescens (gagnep.) c.y. w ...201123961108
ethnobotanical study of antilithic plants of bhopal district.since urolithiasis is the third most common disease, found throughout the world, with a prevalence of 1-5% in asia and two stone belts have been identified in india, one of which includes the study area, so an ethnobotanical study was conducted in and around the bhopal district of madhya pradesh, india to investigate the range of plant species used in the treatment of urolithiasis.201526253579
antioxidative and antidiabetic activities of watermelon (citrullus lanatus) juice on oxidative stress in alloxan-induced diabetic male wistar albino rats.the nutritional and medicinal importance of watermelon has been emphasized and its diseases preventive and curative power must be evaluated. hence, this study was designed to evaluate the antioxidative and antidiabetic potentials of watermelon.201726759513
structure, evolution and functional inference on the mildew locus o (mlo) gene family in three cultivated cucurbitaceae spp.the powdery mildew disease affects thousands of plant species and arguably represents the major fungal threat for many cucurbitaceae crops, including melon (cucumis melo l.), watermelon (citrullus lanatus l.) and zucchini (cucurbita pepo l.). several studies revealed that specific members of the mildew locus o (mlo) gene family act as powdery mildew susceptibility factors. indeed, their inactivation, as the result of gene knock-out or knock-down, is associated with a peculiar form of resistance, ...201526715041
the genomic and biological characterization of citrullus lanatus cryptic virus infecting watermelon in china.a dsrna virus was detected in the watermelon (citrullus lanatus) samples collected from kaifeng, henan province, china through the use of next generation sequencing of small rnas. the complete genome of this virus is comprised of dsrna-1 (1603nt) and dsrna-2 (1466nt), both of which are single open reading frames and potentially encode a 54.2kda rna-dependent rna polymerase (rdrp) and a 45.9kda coat protein (cp), respectively. the rdrp and cp share the highest amino acid identities 85.3% and 75.4 ...201728238875
local melatonin application induces cold tolerance in distant organs of citrullus lanatus l. via long distance transport.melatonin is a ubiquitous chemical substance that regulates plant growth and responses to stress. several recent studies show that exogenous melatonin confers cold tolerance to plants; however, the underlying mechanisms remain largely unknown. here, we report that melatonin application at optimal dose, either on the leaves or the roots, not only induced cold stress tolerance in the site of application, but also systemically induced cold tolerance in untreated distant parts. foliar or rhizospheri ...201728102304
comparative transcriptome analysis of two contrasting watermelon genotypes during fruit development and ripening.watermelon [citrullus lanatus (thunb.) matsum. & nakai] is an economically important crop with an attractive ripe fruit that has colorful flesh. fruit ripening is a complex, genetically programmed process.201728049426
transcriptome profiling of watermelon root in response to short-term osmotic stress.osmotic stress adversely affects the growth, fruit quality and yield of watermelon (citrullus lanatus (thunb.) matsum. & nakai). increasing the tolerance of watermelon to osmotic stress caused by factors such as high salt and water deficit is an effective way to improve crop survival in osmotic stress environments. roots are important organs in water absorption and are involved in the initial response to osmosis stress; however, few studies have examined the underlying mechanism of tolerance to ...201627861528
identification of appropriate reference genes for normalization of mirna expression in grafted watermelon plants under different nutrient stresses.watermelon (citrullus lanatus) is a globally important crop belonging to the family cucurbitaceae. the grafting technique is commonly used to improve its tolerance to stress, as well as to enhance its nutrient uptake and utilization. it is believed that mirna is most likely involved in its nutrient-starvation response as a graft-transportable signal. the quantitative real-time reverse transcriptase polymerase chain reaction is the preferred method for mirna functional analysis, in which reliable ...201627749935
protective effects of seed melon extract on ccl4-induced hepatic fibrosis in mice.citrullus lanatus ssp. vulgaris var. megalaspermus lin et chao, was also known as watermelon belongs to family cucurbitaceae, variously used as healthy food and in the treatment of liver and lungs problems. currently, citrullus lanatus has become a major economic crop of medicinal and edible effects with regional characteristics.201627717904
high-throughput microrna and mrna sequencing reveals that micrornas may be involved in melatonin-mediated cold tolerance in citrullus lanatus l.transcriptional regulation of cold-responsive genes is crucial for exogenous melatonin-mediated cold tolerance in plants. nonetheless, how melatonin regulates cold-responsive genes is largely unknown. in this study, we found that exogenous melatonin improved cold tolerance in watermelon by regulating expression of micrornas (mirnas). we identified a set of mirnas that were regulated by melatonin under unstressed or cold conditions. importantly, mrna-seq analysis revealed that melatonin-induced d ...201627574526
genome wide characterization of simple sequence repeats in watermelon genome and their application in comparative mapping and genetic diversity analysis.microsatellite markers are one of the most informative and versatile dna-based markers used in plant genetic research, but their development has traditionally been difficult and costly. the whole genome sequencing with next-generation sequencing (ngs) technologies provides large amounts of sequence data to develop numerous microsatellite markers at whole genome scale. ssr markers have great advantage in cross-species comparisons and allow investigation of karyotype and genome evolution through h ...201627495254
genome-wide identification of two-component system genes in cucurbitaceae crops and expression profiling analyses in cucumber.cucumber and watermelon, which belong to cucurbitaceae family, are economically important cultivated crops worldwide. however, these crops are vulnerable to various adverse environments. two-component system (tcs), consisting of histidine kinases (hks), phosphotransfers (hps), and response regulator proteins (rrs), plays important roles in various plant developmental processes and signaling transduction in responses to a wide range of biotic and abiotic stresses. no systematic investigation has ...201627446129
fruit quality assessment of watermelons grafted onto citron melon rootstock.the grafting of watermelons (citrullus lanatus) is a common technique that increases yield under stressful soil conditions. the most common rootstocks for watermelons are cucurbita hybrids. however, they often have a negative impact on fruit quality. exploiting novel citrullus germplasm such as citron melon (citrullus lanatus var. citroides) is an alternative to avoid these quality problems.201727436661
phyto-mediated biosynthesis of silver nanoparticles using the rind extract of watermelon (citrullus lanatus) under photo-catalyzed condition and investigation of its antibacterial, anticandidal and antioxidant efficacy.the biological synthesis of nanoparticles has gained tremendous interest, and plants and plant extracts are preferred over other biological sources for this process because of their rich content of bioactive metabolites. in this study, silver nanoparticles (agnps) were produced utilizing the aqueous extract of watermelon rind (wra), an agricultural waste material under photo exposed condition at room temperature, and tested for their antibacterial, anticandidal and antioxidant activities. the sy ...201627261701
development of cleaved amplified polymorphic sequence markers and a caps-based genetic linkage map in watermelon (citrullus lanatus [thunb.] matsum. and nakai) constructed using whole-genome re-sequencing data.cleaved amplified polymorphic sequence (caps) markers are useful tools for detecting single nucleotide polymorphisms (snps). this study detected and converted snp sites into caps markers based on high-throughput re-sequencing data in watermelon, for linkage map construction and quantitative trait locus (qtl) analysis. two inbred lines, cream of saskatchewan (cos) and lsw-177 had been re-sequenced and analyzed by perl self-compiled script for caps marker development. 88.7% and 78.5% of the assemb ...201627162496
the effects of cattle manure and garlic rotation on soil under continuous cropping of watermelon (citrullus lanatus l.).continuous cropping of watermelon (citrullus lanatus l.) can lead to reduced yield and quality. we aimed to determine the effects of cattle manure addition and rotation with green garlic to improve yield and reduce disease incidence in watermelon and to examine the effects on the biological and chemical characteristics of the soil. field experiments were performed during 2012-2014 on land previously under two years of continuous watermelon cropping in northwest china. we examined three treatment ...201627258145
proteomics and metabolomics analyses reveal the cucurbit sieve tube system as a complex metabolic space.the plant vascular system, and specifically the phloem, plays a pivotal role in allocation of fixed carbon to developing sink organs. although the processes involved in loading and unloading of sugars and amino acids are well characterized, little information is available regarding the nature of other metabolites in the sieve tube system (sts) at specific sites along the pathway. here, we elucidate spatial features of metabolite composition mapped with phloem enzymes along the cucurbit sts. phlo ...201627155400
the ethylene biosynthesis gene citacs4 regulates monoecy/andromonoecy in watermelon (citrullus lanatus).monoecious and andromonoecious cultivars of watermelon are characterised by the production of male and female flower or male and hermaphrodite flowers, respectively. the segregation analysis in the offspring of crosses between monoecious and andromonoecious lines has demonstrated that this trait is controlled by a single gene pair, being the monoecious allele m semi-dominant to the andromonoecious allele a. the two studied f1 hybrids (ma) had a predominantly monoecious phenotype since both produ ...201627149159
oviposition preferences of pickleworm (lepidoptera: crambidae) in relation to a potential push-pull cropping management approach.pickleworm, diaphania nitidalis cramer (lepidoptera: crambidae), is a major pest of cucurbits. the current management approach for this pest is weekly insecticide applications. a push-pull cropping approach may be an alternative management practice and could reduce reliance on pesticides. one potential push-pull scenario is the use of squash (cucurbita pepo l.) as a trap crop and watermelon (citrullus lanatus [thunb.] matsum. & nakai) as a deterrent intercrop to manage pickleworm on cantaloupe ( ...201627118111
genome-wide identification and expression analysis of the cltcp transcription factors in citrullus lanatus.the plant-specific tcp transcription factor family, which is involved in the regulation of cell growth and proliferation, performs diverse functions in multiple aspects of plant growth and development. however, no comprehensive analysis of the tcp family in watermelon (citrullus lanatus) has been undertaken previously.201627072931
spatial accumulation pattern of citrulline and other nutrients in immature and mature watermelon fruits.watermelon (citrullus lanatus l.) originates from arid regions of southern africa, and its fruit contains a large amount of the amino acid citrulline, an efficient hydroxyl radical scavenger. citrulline is implicated in the production of nitric oxide in human endothelium, and potential health benefits including vasodilatation and antioxidant functions have been suggested. however, citrulline metabolism in watermelon fruits is poorly understood.201727060681
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