effects of an aqueous extract of cotton seed (gossypium barbadense linn.) on adult male rats.twenty adult male rats per group in 4 treatment groups were injected intraperitoneally at 08.00 hours with 0.1 ml of an aqueous cotton seed extract (gossypium barbadense linn.) (malvaceae) in concentrations of (a) 105.25, (b) 21.21, (c) 4.65, (d) 2.325 mg ml-1 (kg body weight)-1, respectively. a fifth group (control) was given 0.1 ml of pyrogen free distilled water per rat. five rats per treatment group were sacrificed at 2, 8, 24 and 168 hours respectively after treatment. plasma follicle stimu ...19911776560
survey of the mycoflora and mycotoxins of cotton seeds and cotton seed products in egypt.thirty-nine species and 16 fungal genera were isolated from egyptian cotton seeds, cotton seed meal and cotton seed cake on 1% glucose-czapek's agar medium incubated at 28 degrees c. aspergillus was the most frequent genus and it emerged in 87-100% of the samples contributing 70-98% of total fungi in the three substrates tested. the most common species were a. niger, a. flavus, a. fumigatus, a. terreus and rhizopus stolonifer; a. niger, a. fumigatus and penicillium corylophilum; and a. niger, a. ...19902388680
cell wall synthesis in cotton roots after infection with fusarium oxysporum. the deposition of callose, arabinogalactans, xyloglucans, and pectic components into walls, wall appositions, cell plates and plasmodesmata.fusarium oxysporum f. sp. vasinfectum penetration hyphae infect living cells in the meristematic zone of cotton (gossypium barbadense l.) roots. we characterized wall modifications induced by the fungus during infection of the protodermis using antibodies against callose, arabinogalactan-proteins, xyloglucan, pectin, polygalacturonic acid and rhamnogalacturonan i in high-pressure frozen, freeze-substituted root tissue. using quantitative immunogold labelling we compared the cell walls before and ...19958580765
a synthetic cryic gene, encoding a bacillus thuringiensis delta-endotoxin, confers spodoptera resistance in alfalfa and tobacco.spodoptera species, representing widespread polyphagous insect pests, are resistant to bacillus thuringiensis delta-endotoxins used thus far as insecticides in transgenic plants. here we describe the chemical synthesis of a cryic gene by a novel template directed ligation-pcr method. this simple and economical method to construct large synthetic genes can be used when routine resynthesis of genes is required. chemically phosphorylated adjacent oligonucleotides of the gene to be synthesized are a ...19968986755
the in vivo production of spodoptera littoralis nuclear polyhedrosis virus.the in vivo production of the nucleopolyhedrovirus (npv) of the egyptian cotton leafworm spodoptera littoralis was studied experimentally. larvae (7 days old) of 30-50 mg were experimentally infected with a range of npv doses then harvested alive at various times after dosing to determine the effect of dose and incubation time on npv productivity. maximum npv production achieved after 7 days incubation was 1.86 x 10(9) polyhedral inclusion bodies (pibs) per larvae using an inoculum of 1 x 10(4) ...19989628227
cloning and expression of the lepidopteran toxin produced by bacillus thuringiensis var. thuringiensis in escherichia coli.the bacillus thuringiensis var. thuringiensis strain 3a produces a proteinaceous parasporal crystal toxic to larvae of a variety of lepidopteran pests including spodoptera littoralis (egyptian cotton leaf worm), heliothis zeae, h. virescens and boarmia selenaria. by cloning of individual plasmids of b. thuringiensis in escherichia coli, we localized a gene coding for the delta-endotoxin on the b. thuringiensis plasmid of about 17 kb designated ptn4. following partial digestion of the b. thuringi ...19863013729
development and field performance of a broad-spectrum nonviable asporogenic recombinant strain of bacillus thuringiensis with greater potency and uv resistance.the main problems with bacillus thuringiensis products for pest control are their often narrow activity spectrum, high sensitivity to uv degradation, and low cost effectiveness (high potency required). we constructed a sporulation-deficient sigk(-) b. thuringiensis strain that expressed a chimeric cry1c/ab gene, the product of which had high activity against various lepidopteran pests, including spodoptera littoralis (egyptian cotton leaf worm) and spodoptera exigua (lesser [beet] armyworm), whi ...199910473413
antagonism between rhizoctonia solani causing damping-off and selected rhizospheric microflora of some egyptian cotton varieties. 19654961247
comparative analysis of the granulin regions of the phthorimaea operculella and spodoptera littoralis granuloviruses.the nucleotide sequence of two cloned restriction fragments encompassing the granulin genes from the granuloviruses of the potato tuber moth, phthorimaea operculella, phopgv, and the egyptian cotton leaf worm, spodoptera littoralis, spligv, have been determined. although both viruses are able to infect the same ph. operculella cell line, their granulins do not cluster in the same phylogenetic branches. phopgv ganulin is closely related to cydia pomonella gv (cpgv) and cryptophlebia leucotreta gv ...200011129630
the cyclization of farnesyl diphosphate and nerolidyl diphosphate by a purified recombinant delta-cadinene synthase.the first step in the conversion of the isoprenoid intermediate, farnesyl diphosphate (fdp), to sesquiterpene phytoalexins in cotton (gossypium barbadense) plants is catalyzed by delta-cadinene (cdn) synthase. cdn is the precursor of desoxyhemigossypol and hemigossypol defense sesquiterpenes. in this paper we have studied the mechanism for the cyclization of fdp and the putative intermediate, nerolidyl diphosphate, to cdn. a purified recombinant cdn synthase (cdn1-c1) expressed in escherichia co ...200111299356
cloning and expression of desoxyhemigossypol-6-o-methyltransferase from cotton (gossypium barbadense).terpenoids play an important role in defense against insects and pathogens in cotton. these terpenoids contain phenolic groups. metabolites in which the phenolic group has been converted to a methoxy group are less toxic to most insects and pathogens and thus may alter resistance. here is reported the cloning of a gene from gossypium barbadense that encodes the enzyme that methylates the phenolic group of desoxyhemigossypol (dhg) exclusively at the 6-position, dhg-6-o-methyltransferase (dhg-6-om ...200212009981
insecticidal activity of selected actinomycete strains against the egyptian cotton leaf worm spodoptera littoralis (lepidoptera: noctuidae).actinomycete strains isolated from soil samples of saudi arabia and egypt were used in the present study to investigate the biological activity of their secondary metabolites on the cotton leaf worm spodoptera littoralis. secondary metabolites of 41 strains were given to the newly molted last instar larvae through the food plant (castor leaves). many actinomycete strains (58% of all) caused larval mortality ranging from 10-60%. treatments with strains no. 7, 36, 41 resulted in significant prolon ...200112425071
tylenchida associated with different crops in sennar state (sudan).a study was done on the taxonomy and morphology of plant parasitic nematodes (tylenchida) found in sennar state (sudan). sixty samples of different crops were collected in the sugarcane area. thirty samples originated from soil around the roots of saccharum officinarum (sugarcane) from different ratoons and thirty samples were collected from other crops (mangifera indica; citrus limon; citrus aurantifolia; citrus paradisi; citrus sinensis, phoenix dactylifera, musa sapentium; cassia italica, cap ...200112425088
enzymatic changes and cytotoxic effects of metarhizium anisopliae fungal extracts on the egyptian cotton leaf worm spodoptera littoralis (sl 96) cell line.the extra-and intra-cellular extracts of two metarhizium anisopliae isolates: ma23 and mr3, were introduced to a cell line of spodoptera littoralis. the cell line (sl96) was established in the entomovirology centre from s. littoralis ovaries. mr3 extracellular fluid-treated culture had an increase in the acid phosphatase (acp) level as compared either to the untreated cell line or to the fungal extract alone. moreover, the enzymatic tests showed that alpha and beta-glucosidases have been detecte ...200212512818
growth responses of fusarium oxysporum to metabolites of some rhizospheric microflora of egyptian cotton verieties. 195813517230
opposite effects on spodoptera littoralis larvae of high expression level of a trypsin proteinase inhibitor in transgenic plantsthis work illustrates potential adverse effects linked with the expression of proteinase inhibitor (pi) in plants used as a strategy to enhance pest resistance. tobacco (nicotiana tabacum l. cv xanthi) and arabidopsis [heynh.] ecotype wassilewskija) transgenic plants expressing the mustard trypsin pi 2 (mti-2) at different levels were obtained. first-instar larvae of the egyptian cotton worm (spodoptera littoralis boisd.) were fed on detached leaves of these plants. the high level of mti-2 expre ...19989808744
circadian mating activity and effect of pheromone pre-exposure on pheromone response rhythms in the moth spodoptera littoralis.mating in moths is generally mediated by female-produced sex pheromones. mating activity, female pheromone production/release and male pheromone responsiveness all show diurnal variations in many species. we found that the response of the male egyptian cotton leafworm, spodoptera littoralis, to sex pheromone gland extracts showed a diel rhythm in olfactometer tests, and the variation was persistent for at least 1 day in constant darkness. high male response to sex pheromone was correlated in tim ...200515749110
influence of the nonsteroidal ecdysone agonist, tebufenozide, on certain biological and physiological parameters of the cotton leaf-worm, spodoptera littoralis (boisd.) (noctuidae: lepidoptera) in egypt, cotton represents the most economical and important field crop. cotton plant is the target of different pest species from cultivation until harvest. the egyptian cotton leaf-worm, spodoptera littoralis (boisd.) (noctuidae: lepidoptera), is an important polyphagous insect pest attacking cotton, several cultivated crops and ornamentals worldwide. the integrated pest management (ipm) programs create the need for novel insecticides having more selective modes of action. so, the no steroida ...200415759403
[identification and characterization of differentially expressed ests of gossypium barbadense infected by verticillium dahliae with suppression subtractive hybridization].cotton wilt defense reaction is a complicated continuous process and involves a battery of genes. in this study, we adopted suppression subtractive hybridization (ssh) technique to isolate differentially expressed ests from gossypium barbadense variety 7124 during verticillium wilt defense process. an array of 1165 clones from the subtractive library has been screened with reverse northern blotting, of which 131 ests were considered as over-expressed and 16 ests were down-regulated. sequence ana ...200515856944
[construction and analysis of ssh library of gossypium barbadense upon infection with verticillium dahliae].roots were collected from the seedlings inoculated with pathogen verticillium dahliae after 2, 4, 8, 12, 24, 48, 72 and 96 hours for total rna extraction. the cdnas from the inoculated seedlings were used as the tester and those from the control seedlings as the driver. ssh method was employed to find the differently expressed cdnas responding to the pathogen. t/a clone library was constructed containing 534 clones. the cdna inserts were amplified from the bacterial clones directly with m13 prim ...200516018265
wide-cross whole-genome radiation hybrid mapping of the cotton (gossypium barbadense l.) genome.whole-genome radiation hybrid mapping has been applied extensively to human and certain animal species, but little to plants. we recently demonstrated an alternative mapping approach in cotton (gossypium hirsutum l.), based on segmentation by 5-krad gamma-irradiation and derivation of wide-cross whole-genome radiation hybrids (wwrhs). however, limitations observed at the 5-krad level suggested that higher doses might be advantageous. here, we describe the development of an improved second-genera ...200616362372
isolation and characterization of resistance and defense gene analogs in cotton (gossypium barbadense l.).plant disease resistance gene (r gene) and defense response gene encode some conserved motifs. in the present work, a pcr strategy was used to clone resistance gene analogs (rgas) and defense gene analogs (dgas) from sea-island cotton variety hai7124 using oligonucleotide primers based on the nucleotide-binding site (nbs) and serine/threonine kinase (stk) in the r-gene and pathogenesis-related proteins of class 2 (pr2) of defense response gene. 79 nbs sequences, 21 stk sequences and 11 dgas were ...200617312991
differential induction of 3-hydroxy-3-methylglutaryl coa reductase in two cotton species following inoculation with verticillium.gossypium barbadense cottons are typically more resistant to wilt pathogens than are gossypium hirsutum cultivars. both species make terpenoid phytoalexins in response to infection, implicating isoprenoid biosynthesis as a factor in resistance. conserved regions in plant 3-hydroxy-3-methylglutaryl coenzyme a reductase (hmgr), the first enzyme in the terpene biosynthesis pathway, were used to design polymerase chain reaction primers for cloning a fragment of a cotton hmgr gene. the clone was used ...19958664497
a potato carboxypeptidase inhibitor gene provides pathogen resistance in transgenic rice.a defensive role against insect attack has been traditionally attributed to plant protease inhibitors. here, evidence is described of the potential of a plant protease inhibitor, the potato carboxypeptidase inhibitor (pci), to provide resistance to fungal pathogens when expressed in rice as a heterologous protein. it is shown that rice plants constitutively expressing the pci gene exhibit resistance against the economically important pathogens magnaporthe oryzae and fusarium verticillioides. a m ...200717547659
medicinal plants in suriname: hypotensive effect of gossypium traditional medicine gossypium barbadense l. is used against hypertension. looking for a scientific basis for this use, the blood-pressure-lowering effect of the decoction of the leaves was confirmed. fraction ii (frii) of the crude extract of g. barbadense showed a dose-dependent hypotensive effect in anaesthetized rats. in hexamethonium-treated rats, the blood-pressure-lowering effect of frii was almost abolished. a small decrease of the blood-pressure-lowering effect was followed by an inc ...200415025864
plant crude extracts could be the solution: extracts showing in vivo antitumorigenic activity.screening active compounds from plants lead to discover new medicinal drugs which have efficient protection and treatment roles against various diseases including cancer. in our study, extracts from different plants represent seeds of: gossypium barbadense, ricinus communis, sesamum indicum, nigella sativa, vinca rosea and melia azedarah; fruits of: xanthium occidental; flowers of: atriplex nummularia; barks of: cinnamomum zeylanicum; latex of: ficus carica and rhizomes of: curcuma longa and zin ...200818390447
biological performance of quercetin on the cotton leaf-worm larvae, spodoptera littoralis boisd. (lep., noctuidae) and prevailing natural enemies in the egyptian cotton fields.the egyptian cotton (gossypium barbadense l.) accounts for 65 % of the world production of long stable cultivars. taking into consideration the competition of other cotton producing countries, it should be of great importance to control pests, which attack the cotton plants to improve the yield and its quality. the main objective of this study is to develop new approaches for the management of the cotton leafworm spodoptera littoralis boisd. within an ipm program, that include synthetic insectic ...200718399494
global analysis of gene expression in cotton fibers from wild and domesticated gossypium barbadense.gossypium barbadense is widely cultivated because of its extra-long staple cotton with superior luster, silkiness and high yield. these economically important traits were selected during initial domestication of an agronomically inferior wild ancestor, followed by millennia of human-mediated selection. to reveal the effects of this history on the cotton fiber transcriptome, we conducted comparative expression profiling on mechanically isolated fiber cells at three different stages encompassing e ...200818803775
a fusarium wilt resistance gene in gossypium barbadense and its effect on root-knot nematode-wilt disease complex.abstract fusarium wilt, caused by the soilborne pathogen fusarium oxysporum f. sp. vasinfectum race 1, is a vascular disease in cotton (gossypium spp.), and is a component of a disease complex with root-knot nematodes (meloidogyne incognita). genetic analysis of two interspecific crosses (g. barbadense pima s-7 x g. hirsutum acala nemx and pima s-7 x acala sj-2) showed that one major gene (designated fov1) with allele dosage effect conferred resistance to f. oxysporum f. sp. vasinfectum race 1 i ...200618943146
molecular cloning, expression profiling and functional analyses of a cdna encoding isopentenyl diphosphate isomerase from gossypium barbadense.gossypol, a type of plant defence sesquiterpenoid phytoalexin, is synthesized from the mep (2c-methyl-d-erythritol 4-phosphate) and mva (mevalonate) pathway in the isoprenoid biosynthetic system. the key step is the isomerization of ipp (isopentenyl diphosphate) to dmapp (dimethylallyl diphosphate), which is catalysed by ipi (ipp isomerase; ec a full-length cdna encoding ipi (designated gbipi) was cloned from gossypium barbadense by race (rapid amplification of cdna ends). the full-len ...200919055484
starch grains on human teeth reveal early broad crop diet in northern peru.previous research indicates that the nanchoc valley in northern peru was an important locus of early and middle holocene human settlement, and that between 9200 and 5500 (14)c yr b.p. the valley inhabitants adopted major crop plants such as squash (cucurbita moschata), peanuts (arachis sp.), and cotton (gossypium barbadense). we report here an examination of starch grains preserved in the calculus of human teeth from these sites that provides direct evidence for the early consumption of cultivat ...200819066222
induction of delta-cadinene synthase and sesquiterpenoid phytoalexins in cotton by verticillium dahliae.phytoalexin biosynthesis occurred earlier in the resistant cotton cultivar seabrook sea island 12b2 (sbsi) (gossypium barbadense) than in the susceptible cotton cultivar rowden (g. hirsutum) after inoculation with a defoliating isolate of the pathogen verticillium dahliae. this was demonstrated by significantly higher levels of phytoalexins in sbsi 12 h after inoculation. furthermore, by 48 h after inoculation of sbsi, the phytoalexins hemigossypol and desoxyhemigossypol achieved levels (23.9 an ...199910552825
over-expression gberf2 transcription factor in tobacco enhances brown spots disease resistance by activating expression of downstream genes.erf transcription factors can bind gcc boxes or non-gcc cis elements to regulate biotic and abiotic stress responses. here, we report that an erf transcription factor gene (gberf2) was cloned by suppression subtraction hybridization from sea-island cotton after verticillium dahliae attack. the gberf2 cdna has a total length of 1143 bp with an open reading frame of 597 bp. the genomic sequence of gberf2 contains an intron of 515 bp. the gene encodes a predicated polypeptide of 198 amino acids wit ...200717321073
resistance to meloidogyne incognita race 3 and rotylenchulus reniformis in wild accessions of gossypium hirsutum and g. barbadense from mexico.forty-six accessions of g. hirsutum and two of g. barbadense were examined for resistance to meloidogyne incognita race 3 and rotylenchulus reniformis in environmental growth chamber experiments, with the objective of finding new sources of resistance. only the g. barbadense accessions, tx-1347 and tx-1348, supported significantly less reproduction by r. reniformis than the susceptible control, deltapine 16 (usda accession sa-1186). however, they were highly susceptible to m. incognita race 3. t ...199719274280
resistance to bacterial blight of cotton varieties homozygous for combinations of b resistance the sudan, inoculation experiments were done on a wide range of egyptian cotton lines homozygous for single genes and for digenic and trigenic combinations of knight's b genes for resistance to bacterial blight. although leaf inoculation was successful, stem inoculation was only partially so and boll inoculation, using two different techniques, failed to produce measurable disease symptoms. there was a good general relationship between leaf and stem resistance, and a close association between ...197419280793
potential of "lure and kill" in long-term pest management and eradication of invasive species."lure and kill" technology has been used for several decades in pest management and eradication of invasive species. in lure and kill, the insect pest attracted by a semiochemical lure is not "entrapped" at the source of the attractant as in mass trapping, but instead the insect is subjected to a killing agent, which eliminates affected individuals from the population after a short period. in past decades, a growing scientific literature has been published on this concept. this article provides ...200919610395
a thaumatin-like protein gene involved in cotton fiber secondary cell wall development enhances resistance against verticillium dahliae and other stresses in transgenic tobacco.for the first time, a sea-island cotton (gossypium barbadense l.) thaumatin-like protein gene (gbtlp1) with a potential role in secondary cell wall development has been overexpressed in tobacco to elucidate its function. the presence of the transgene was verified by southern blotting and higher expression levels of gbtlp1 in transgenic tobacco plants were revealed by reverse-transcription and quantitative real-time polymerase chain reaction analyses. transgenic plants with constitutively higher ...201020097164
preceramic adoption of peanut, squash, and cotton in northern peru.the early development of agriculture in the new world has been assumed to involve early farming in settlements in the andes, but the record has been sparse. peanut (arachis sp.), squash (cucurbita moschata), and cotton (gossypium barbadense) macrofossils were excavated from archaeological sites on the western slopes of the northern peruvian andes. direct radiocarbon dating indicated that these plants grew between 9240 and 5500 (14)c years before the present. these and other plants were recovered ...200717600214
identification of qtl regions and ssr markers associated with resistance to reniform nematode in gossypium barbadense l. accession gb713.the identification of molecular markers that are closely linked to gene(s) in gossypium barbadense l. accession gb713 that confer a high level of resistance to reniform nematode (rn), rotylenchulus reniformis linford & oliveira, would be very useful in cotton breeding programs. our objectives were to determine the inheritance of rn resistance in the accession gb713, to identify ssr markers linked with rn resistance qtls, and to map these linked markers to specific chromosomes. we grew and scored ...201120845024
preliminary studies for the development of a second generation granulocytapheresis (g-cap) column.the preliminary studies for developing a second generation granulocytapheresis (g-cap) column were made. in the past, the g-cap column has been used for the treatment of ulcerative colitis and rheumatoid arthritis. however, recent clinical studies have revealed that the therapeutic effects of the g-cap column are not significant compared with those of the sham column. these results were considered to be due to insufficient reduction of granulocytes. thus, development of a better granulocyte remo ...201020364286
study of the bacillus thuringiensis vip3aa16 histopathological effects and determination of its putative binding proteins in the midgut of spodoptera littoralis.the bacterium bacillus thuringiensis produces, at the vegetative stage of its growth, vip3a proteins with activity against a broad spectrum of lepidopteran insects. the egyptian cotton leaf worm (spodoptera littoralis) is an important agricultural pest that is susceptible to the vip3aa16 protein of bacillus thuringiensis kurstaki strain bupm95. the midgut histopathology of vip3aa fed larvae showed vacuolization of the cytoplasm, brush border membrane destruction, vesicle formation in the apical ...201020965198
opportunistic nature of the mammalian microsporidia: experimental transmission of trachipleistophora extenrec (fungi: microsporidia) between mammalian and insect hosts.spores of trachipleistophora extenrec, originally isolated from the muscles of the madagascan insectivore hemicentetes semispinosus and maintained by serial passage in severe combined immunodeficiency (scid) mice, were fed to larvae of the egyptian cotton leafworm spodoptera littoralis. extensive infection of larval tissues ensued and caused larval and pupal mortality. the development of t. extenrec in the insect host, studied both by light and electron microscopy, followed generally the same li ...201021188601
molecular cloning and characterization of the actin-depolymerizing factor gene in gossypium barbadense.the sea island cotton (gossypium barbadense l.) has been highly valued in verticillium wilt resistance and many fiber qualities including fiber length, strength and fineness. to identify whether it had some special genes in fiber development in comparison with the upland cotton (g. hirsutum l.), an actin-depolymerizing factor (adf) gene was cloned and characterized in this research. a 420 bp open reading frame of the cloned gene, named gbadf1, encoded a protein of 139 amino acids, including 39.5 ...200819168989
the effects of different prey regimes on the proteolytic digestion of nymphs of the spined soldier bug, podisus maculiventris (hemiptera: pentatomidae).the effects of different prey regimes on the performance and digestive physiology of the spined soldier bug, podisus maculiventris (say) (hemiptera: pentatomidae), were assessed. specifically, p. maculiventris nymphs were fed on colorado potato beetle (cpb), leptinotarsa decemlineata say (coleoptera: chrysomelidae), larvae; egyptian cotton leafworm (ecw); spodoptera littoralis (boisduval) (lepidoptera: noctuidae); larvae; calliphora spp. (cal) (diptera: calliphoridae) pupae or a mixture of the t ...200919203402
cloning and characterization of a verticillium wilt resistance gene from gossypium barbadense and functional analysis in arabidopsis thaliana.verticillium wilt causes enormous loss to yield or quality in many crops. in an effort to help controlling this disease through genetic engineering, we first cloned and characterized a verticillium wilt resistance gene (gbve) from cotton (gossypium barbadense) and analyzed its function in arabidopsis thaliana. its nucleotide sequence is 3,819-ábp long, with an open reading frame of 3,387-ábp, and encoding an 1,128-aa protein precursor. sequence analysis shows that gbve produces a leucine-rich re ...201121739145
molecular cloning and expression analysis of a novel sant/myb gene from gossypium barbadense.myb family transcription factors are implicated in multiple developmental processes. herein, a new full-length cdna encoding a sant/myb transcription factor (designated as gbrl2) was cloned and characterized from cotton (gossypium barbadense l.) for the first time. the full-length cdna of gbrl2 was 573 bp with a 240 bp open reading frame (orf) encoding a deduced protein of 80 amino acid polypeptide with a calculated molecular mass of 8.96 kda and an isoelectric point of 8.96. sequence alignment ...201021069464
expression of s-adenosyl methionine decarboxylase gene for polyamine accumulation in egyptian cotton giza 88 and giza 90.developing drought tolerance in egyptian cotton varieties is a strategic goal considering the need to expand cotton cultivated area and water scarcity in the nile valley. in the present study, increasing levels of polyamine accumulation via expressing s-adenosyl methionine decarboxylase (samdc) gene was the main goal. samdc cdna isolated from saccharomyces cerevisiae isolate was isolated and genetically engineered into egyptian cotton varieties giza 88 as an extra long staple and giza 90 as a lo ...201021844681
The impact of the Bacillus subtilis SPB1 biosurfactant on the midgut histology of Spodoptera littoralis (Lepidoptera: Noctuidae) and determination of its putative receptor.SPB1 is a Bacillus subtilis strain producing a lipopeptide biosurfactant. The insecticidal activity of this biosurfactant was evaluated against the Egyptian cotton leaf worm (Spodoptera littoralis). It displayed toxicity with an LC(50) of 251ng/cm(2). The histopathological changes occurred in the larval midgut of S. littoralis treated with B. subtilis SPB1 biosurfactant were vesicle formation in the apical region, cellular vacuolization and destruction of epithelial cells and their boundaries. L ...201122079884
Proteomic analysis of the sea-island cotton roots infected by wilt pathogen Verticillium dahliae.Verticillium wilt of cotton is a vascular disease mainly caused by the soil-born filamentous fungus Verticillium dahliae. To study the mechanisms associated with defense responses in wilt-resistant sea-island cotton (Gossypium barbadense) upon V. dahliae infection, a comparative proteomic analysis between infected and mock-inoculated roots of G. barbadense var. Hai 7124 (a cultivar showing resistance against V. dahliae) was performed by 2-DE combined with local EST database-assisted PMF and MS/M ...201121928292
investigation of the steps involved in the difference of susceptibility of ephestia kuehniella and spodoptera littoralis to the bacillus thuringiensis vip3aa16 toxin.bupm95 is a bacillusthuringiensis subsp. kurstaki strain producing the vip3aa16 toxin with an interesting insecticidal activity against the lepidopteran larvae ephestia kuehniella. study of different steps in the mode of action of this vegetative insecticidal protein on the mediterranean flour moth (e. kuehniella) was carried out in the aim to investigate the origin of the higher susceptibility of this insect to vip3aa16 toxin compared to that of the egyptian cotton leaf worm spodoptera littoral ...201121600212
preliminary studies for the development of a second generation granulocytapheresis (g-cap) column. part ii: in vitro and ex vivo studies.our previous studies concluded egyptian cotton was the most appropriate material for making a second generation granulocytapheresis (g-cap) column as structural dimensions of the cotton fibers are able to attract granulocytes. unfortunately, it is considered to be blood incompatible as its fibers are of non-synthetic origins. in this study we examined the alteration of the removal rates of blood cells with different surface modifications of egyptian cotton to enhance its blood compatibility. the ...201121713519
clonal analysis of leaf development in cotton.clonal analysis has been used to describe the cellular parameters of leaf development in american pima cotton (gossypium barbadense). sectors (clones) induced before leaf initiation indicate that the leaf primordium arises from ~100 cells on the flank of the shoot meristem. an analysis of sector frequency during the period of leaf expansion suggests that the rate of cell division is fairly uniform throughout the length of the leaf, but is lower at the margin of the lamina than in intercalary reg ...199821684913
isolation of (-)-gossypol from natural plant.(-)-gossypol has been isolated from cottonseed of g. barbadense l. three gossypium species were studied for the isolation. the study has also revealed a correlation between the optical activity of gossypol isolated and the species of cottonseed used. gossypol isolated from the species g. barbadense is always found to be levorotatory caused by an excess of (-)-gossypol, whereas that from the species g. hirsutum is always dextrorotatory caused by an excess of (+)-gossypol. results of most samples ...20123370996
gbwrky1, a novel cotton (gossypium barbadense) wrky gene isolated from a bacteriophage full-length cdna library, is induced by infection with verticillium dahliae.wrky transcription factor proteins play important roles in diverse stress responses. in this study, we first cloned a novel wrky from our constructed bacteriophage full-length cdna library for cotton (gossypium barbadense). the plants were stressed by exposure to a defoliating strain of verticillium dahliae. the capacity of primary cdna library was 1.28 x 10(6) pfu and the titer of the amplified cdna library was > 10(10) pfu ml(-1). the recombination rate of the library was 94% and average inser ...201223350274
lignin metabolism has a central role in the resistance of cotton to the wilt fungus verticillium dahliae as revealed by rna-seq-dependent transcriptional analysis and histochemistry.the incompatible pathosystem between resistant cotton (gossypium barbadense cv. 7124) and verticillium dahliae strain v991 was used to study the cotton transcriptome changes after pathogen inoculation by rna-seq. of 32 774 genes detected by mapping the tags to assembly cotton contigs, 3442 defence-responsive genes were identified. gene cluster analyses and functional assignments of differentially expressed genes indicated a significant transcriptional complexity. quantitative real-time pcr (qpcr ...201121862479
the phytohormone precursor opda is isomerized in the insect gut by a single, specific glutathione transferase.oxylipins play important roles in stress signaling in plants. the compound 12-oxophytodienoic acid (cis-opda) is an early biosynthetic precursor of jasmonic acid (ja), the key phytohormone orchestrating the plant anti-herbivore defense. when consumed by feeding lepidopteran larvae, plant-derived cis-opda suffers rapid isomerization to iso-opda in the midgut and is excreted in the frass. unlike opda epimerization (yielding trans-opda), the formation of iso-opda is enzyme-dependent, and is catalyz ...200919805297
ectopic expression of a novel ser/thr protein kinase from cotton (gossypium barbadense), enhances resistance to verticillium dahliae infection and oxidative stress in arabidopsis.overexpression of a cotton defense-related gene gbstk in arabidopsis resulted in enhancing pathogen infection and oxidative stress by activating multiple defense-signaling pathways. serine/threonine protein kinase (stk) plays an important role in the plant stress-signaling transduction pathway via phosphorylation. most studies about stk genes have been conducted with model species. however, their molecular and biochemical characterizations have not been thoroughly investigated in cotton. here, w ...201323912851
a novel function for the cathepsin d inhibitor in tomato.proteinaceous aspartic proteinase inhibitors are rare in nature and are described in only a few plant species. one of them corresponds to a family of cathepsin d inhibitors (cdis) described in potato (solanum tuberosum), involving up to 15 isoforms with a high sequence similarity. in this work, we describe a tomato (solanum lycopersicum) wound-inducible protein called jasmonic-induced protein 21 (jip21). sequence analysis of its cdna predicted a putative function as a cdi. the jip21 gene, whose ...200617012408
genome-wide profiling of mirnas and other small non-coding rnas in the verticillium dahliae-inoculated cotton roots.micrornas (mirnas) and small interfering rnas (sirnas) are short (19-25 nucleotides) non-coding rna molecules that have large-scale regulatory effects on development and stress responses in plants. verticillium wilt is a vascular disease in plants caused by the fungal pathogen verticillium dahliae. the objective of this study is to investigate the transcriptional profile of mirnas and other small non-coding rnas in verticillium-inoculated cotton roots. four small rna libraries were constructed f ...201222558219
overexpression of gbrlk, a putative receptor-like kinase gene, improved cotton tolerance to verticillium wilt.verticillium dahliae is a causative fungal pathogen and only a few genes have been identified that exhibit critical roles in disease resistance and few has shown positive effects on the resistance to verticillium wilt in transgenic cotton. we cloned a receptor-like kinase gene (gbrlk) induced by verticillium dahliae (vd) in the disease-resistant cotton gossypium barbadense cv. hai7124. northern blotting revealed that the gbrlk was induced by vd at 96 h after inoculation. the functional gbrlk is ...201526446555
characterization, expression, and functional analysis of a novel nac gene associated with resistance to verticillium wilt and abiotic stress in cotton.elucidating the mechanism of resistance to biotic and abiotic stress is of great importance in cotton. in this study, a gene containing the nac domain, designated gbnac1, was identified from gossypium barbadense l. homologous sequence alignment indicated that gbnac1 belongs to the tern subgroup. gbnac1 protein localized to the cell nucleus. gbnac1 was expressed in roots, stems, and leaves, and was especially highly expressed in vascular bundles. functional analysis showed that cotton resistance ...201627784753
induced plant defense responses against chewing insects. ethylene signaling reduces resistance of arabidopsis against egyptian cotton worm but not diamondback moth.the induction of plant defenses by insect feeding is regulated via multiple signaling cascades. one of them, ethylene signaling, increases susceptibility of arabidopsis to the generalist herbivore egyptian cotton worm (spodoptera littoralis; lepidoptera: noctuidae). the hookless1 mutation, which affects a downstream component of ethylene signaling, conferred resistance to egyptian cotton worm as compared with wild-type plants. likewise, ein2, a mutant in a central component of the ethylene signa ...200011080278
identification, phylogenetic analysis and expression profile of an anionic insect defensin gene, with antibacterial activity, from bacterial-challenged cotton leafworm, spodoptera littoralis.defensins are a well known family of cationic antibacterial peptides (amps) isolated from fungi, plants, insects, mussels, birds, and various mammals. they are predominantly active against gram (+) bacteria, and a few of them are also active against gram (-) bacteria and fungi. all insect defensins belonging to the invertebrate class have a consensus motif, c-x₅₋₁₆-c-x₃-c-x₉₋₁₀-c-x₄₋₇-cx₁-c. only seven amps have already been found in different lepidopteran species. no report was published on the ...201122067477
mosquito feeding affects larval behaviour and development in a moth.organisms are attacked by different natural enemies present in their habitat. while enemies such as parasitoids and predators will kill their hosts/preys when they successfully attack them, enemies such as micropredators will not entirely consume their prey. however, they can still have important consequences on the performance and ecology of the prey, such as reduced growth, increased emigration, disease transmission. in this paper, we investigated the impact of a terrestrial micropredator, the ...201121991329
microbial control of the cotton leafworm spodoptera littoralis (boisd.) by egyptian bacillus thuringiensis isolates.four local bacillus thuringiensis (bt) isolates that had been serologically identified as bt var. kurstaki (btk2, btk3, and btk66) and bt var. mexicanensis (btm27), in addition to two reference strains (4d20 and 4ac1), were laboratory assayed as microbial control agents against the egyptian cotton leafworm spodoptera littoralis (boisd.). polymerase chain reaction (pcr) amplification analysis revealed that each of the six experimental strains carries, at least, a cry1 type gene which expresses a ...201322983675
a screening for antimicrobial activities of caribbean herbal remedies.the tramil program aims to understand, validate and expand health practices based on the use of medicinal plants in the caribbean, which is a "biodiversity hotspot" due to high species endemism, intense development pressure and habitat loss. the antibacterial activity was examined for thirteen plant species from several genera that were identified as a result of tramil ethnopharmacological surveys or were reported in ethnobotanical accounts from puerto rico. the aim of this study was to validate ...201323731467
cloning of a phosphatidylinositol 4-kinase gene based on fiber strength transcriptome qtl mapping in the cotton species gossypium barbadense.sea island cotton (gossypium barbadense) is highly valued for its superior fiber qualities, especially fiber strength. based on a transcript-derived fragment originated from transcriptome qtl mapping, a fiber strength related candidate gene of phosphatidylinositol 4-kinase cdna, designated as gbpi4k, was first cloned, and its expression was characterized in the secondary cell wall thickening stage of g. barbadense fibers. the orf of gbpi4k was found to be 1926 bp in length and encoded a predicte ...201222869083
laboratory evaluation of isaria fumosorosea against spodoptera littoralis.isaria fumosorosea (syn. paecilomyces fumosoroseus) is potentially useful for the biological control of economically important agricultural and forest insect pests. we evaluated efficacy of two strains of this entomopathogenic fungus against last instar larvae and pupae of egyptian cotton leafworm, spodoptera littoralis. the first strain was apopka 97 which is an active ingredient of commercial biopesticide preferal wg (biobest, belgium). the second strain was isolated from cameraria ohridella a ...201223885438
identification of mirnas and their targets in cotton inoculated with verticillium dahliae by high-throughput sequencing and degradome analysis.micrornas (mirnas) are a group of endogenous small non-coding rnas that play important roles in plant growth, development, and stress response processes. verticillium wilt is a vascular disease in plants mainly caused by verticillium dahliae kleb., the soil-borne fungal pathogen. however, the role of mirnas in the regulation of verticillium defense responses is mostly unknown. this study aimed to identify new mirnas and their potential targets that are involved in the regulation of verticillium ...201526133244
proteomic and virus-induced gene silencing (vigs) analyses reveal that gossypol, brassinosteroids, and jasmonic acid contribute to the resistance of cotton to verticillium dahliae.verticillium wilt causes massive annual losses of cotton yield, but the mechanism of cotton resistance to verticillium dahliae is complex and poorly understood. in this study, a comparative proteomic analysis was performed in resistant cotton (gossypium barbadense cv7124) on infection with v. dahliae. a total of 188 differentially expressed proteins were identified by mass spectrometry (maldi-tof/tof) analysis and could be classified into 17 biological processes based on gene ontology annotation ...201324019146
effects of 1,3-dicliloropropene for meloidogyne incognita management on cotton produced under furrow irrigation.field trials were conducted during 1990 to evaluate the effects of preplant soil fumigation with 1,3-dichloropropene (1,3-d) on yield and fiber quality of furrow-irrigated cotton cultivars subjected to high population densities of meloidogyne incognita. we measured the responses of eight upland cotton cultivars with different levels of root-knot nematode resistance and compared the responses of upland and pima cottons. reductions in lint weight ranged from 10 to 52% among cultivars grown in soil ...199319279835
cotton wrky1 mediates the plant defense-to-development transition during infection of cotton by verticillium dahliae by activating jasmonate zim-domain1 expression.plants have evolved an elaborate signaling network to ensure an appropriate level of immune response to meet the differing demands of developmental processes. previous research has demonstrated that della proteins physically interact with jasmonate zim-domain1 (jaz1) and dynamically regulate the interaction of the gibberellin (ga) and jasmonate (ja) signaling pathways. however, whether and how the jaz1-della regulatory node is regulated at the transcriptional level in plants under normal growth ...201425301887
qtl mapping for resistance to root-knot nematodes in the m-120 rnr upland cotton line (gossypium hirsutum l.) of the auburn 623 rnr source.root-knot nematodes meloidogyne incognita (kofoid and white) can cause severe yield loss in cotton (gossypium hirsutum l.). the objectives of this study were to determine the inheritance and genomic location of genes conferring root-knot nematode resistance in m-120 rnr, a highly resistant g. hirsutum line with the auburn 623 rnr source of resistance. utilizing two interspecific f(2) populations developed from the same m-120 rnr by gossypium barbadense (cv. pima s-6) cross, genome-wide scanning ...200616960714
genetic diversity among exotic cotton accessions as for qualitative and quantitative traits.studying genetic diversity among a group of genotypes is important in genetic breeding because identifying hybrid combinations of greater heterotic effect also increases the chance of obtaining plants with favorable allele combinations in an intra-population selection program. the objective of this study was to compare different types of long and extra-long staple cotton and their genetic diversity in relation to the fiber traits and some agronomic traits in order to grant breeding programs. div ...201728198514
a transgressive segregation factor (rkn2) in gossypium barbadense for nematode resistance clusters with gene rkn1 in g. plant resistance is an important strategy for managing root-knot nematode (meloidogyne incognita) in cotton (gossypium l.). here we report evidence for enhanced resistance in interspecific crosses resulting from transgressive segregation of clustered gene loci. recently, a major gene, rkn1, on chromosome 11 for resistance to m. incognita in cv. acala nemx was identified using an intraspecific g. hirsutum cross with susceptible cv. acala sj-2. using interspecific crosses of acala nemxxsuscep ...200817940800
postinfection development of rotylenchulus reniformis on resistant gossypium barbadense accessions.the reniform nematode (rotylenchulus reniformis) causes significant cotton (gossypium hirsutum) losses in the southeastern united states. the research objective was to describe the effects of two resistant g. barbadense lines (cultivar tx 110 and accession gb 713) on development and fecundity of reniform nematode. nematode development and fecundity were evaluated on the resistant lines and susceptible g. hirsutum cultivar deltapine 16 in three repeated growth chamber experiments. nematode develo ...201526941458
steam explosion distinctively enhances biomass enzymatic saccharification of cotton stalks by largely reducing cellulose polymerization degree in g. barbadense and g. this study, steam explosion pretreatment was performed in cotton stalks, leading to 5-6 folds enhancements on biomass enzymatic saccharification distinctive in gossypium barbadense and gossypium hirsutum species. sequential 1% h2so4 pretreatment could further increase biomass digestibility of the steam-exploded stalks, and also cause the highest sugar-ethanol conversion rates probably by releasing less inhibitor to yeast fermentation. by comparison, extremely high concentration alkali (16% na ...201525656866
a novel gossypium barbadense erf transcription factor, gberfb, regulation host response and resistance to verticillium dahliae in tobacco.ethylene-responsive factors (erfs) are commonly considered to play an important role in pathogen defense responses. however, only few of erf members have been characterized in sea island cotton (gossypium barbadense). here, we reported a novel ap2/erf transcription factors gene, named gberfb which was cloned and identified from sea island cotton by race. the expression of gberfb was significantly induced by treatments with ethylene, methyl jasmonate, salicylic acid, wounding, h2o2 and verticilli ...201728250589
establishment and characterization of spodoptera littoralis cell line (sl 96), adapted at 19 degrees c permissive for different baculoviruses.cells from ovarioule tissues of the egyptian cotton leaf worm, spodoptera littoralis (sl 96) were established, identified and characterized comparing with other lepidopteran cell lines using isozymes, karyotyping, rapd pcr, and polyacrylamide gel electrophoresis polypeptide page profile. comparison of the number of chromosomes and page profiles; also the isozymes profiles demonstrate the possibility to distinguish the cell lines by those analyses and having a distinguishable finger print for eve ...200516333885
emamectin, a novel insecticide for controlling field crop pests.emamectin is a macrocyclic lactone insecticide with low toxicity to non-target organisms and the environment, and is considered an important component in pest-management programmes for controlling field crop pests. it is a powerful compound for controlling the cotton bollworm helicoverpa armigera (hübner). a spray concentration of 25 mg ai litre-1 in a cotton field resulted in over 90% suppression of h armigera larvae up to day 28 after treatment, while similar mortality of the egyptian cotton l ...200212449526
physiological and antioxidant responses of cotton and spurred anoda under interference and mild drought.the influence of plant interference and a mild drought on gas exchange and oxidative stress was investigated using potted plants of two cotton species (gossypium hirsutum l. cv. delta pine 5415, and gossypium barbadense l. cv. pima s-7) and spurred anoda (anoda cristata l. schlecht.) of the malvaceae. without interference, cotton and spurred anoda had similar net photosynthesis (pnet) but different pigment profiles. stomatal conductance (gs) and transpiration rate (e) were greater in spurred ano ...200314504299
the amino acid sequence of cytochrome c from abutilon theophrasti medic. and gossypium barbadense l. (cotton).the amino acid sequences of abutilon and gossypium cytochromes c were determined on 1mumol of protein. the molecules consist of 111 residues and are homologous with other mitochondrial plant cytochromes c. experimental details are given in a supplementary paper that has been deposited as supplementary publication sup 50005 at the national lending library for science and technology, boston spa, yorks. ls23 7bq, u.k., from whom copies can be obtained on the terms indicated in biochem. j. (1971), 1 ...19715131735
development of agrobacterium-mediated virus-induced gene silencing and performance evaluation of four marker genes in gossypium barbadense.gossypiumbarbadense is a cultivated cotton species and possesses many desirable traits, including high fiber quality and resistance to pathogens, especially verticilliumdahliae (a devastating pathogen of gossypium hirsutum, the main cultivated species). these elite traits are difficult to be introduced into g. hirsutum through classical breeding methods. in addition, genetic transformation of g. barbadense has not been successfully performed. it is therefore important to develop methods for eval ...201324023833
a cotton annexin protein anxgb6 regulates fiber elongation through its interaction with actin 1.annexins are assumed to be involved in regulating cotton fiber elongation, but direct evidence remains to be presented. here we cloned six annexin genes (anxgb) abundantly expressed in fiber from sea-island cotton (g. barbadense). qrt-pcr results indicated that all six g. barbadense annexin genes were expressed in elongating cotton fibers, while only the expression of anxgb6 was cotton fiber-specific. yeast two hybridization and bifc analysis revealed that anxgb6 homodimer interacted with a cott ...201323750279
an ethylene response-related factor, gberf1-like, from gossypium barbadense improves resistance to verticillium dahliae via activating lignin ethylene response-related factor, gberf1-like, from gossypium barbadense cv. '7124' involved in the defence response to verticillium dahliae was characterized. gberf1-like transcripts present ubiquitously in various tissues, with higher accumulation in flower organs. gberf1-like was also responsive to defence-related phytohormones and v. dahliae infection. the downregulation of gberf1-like increased the susceptibility of cotton plants to v. dahliae infection, while overexpression of this gene ...201626971283
molecular cloning, characterization and expression analysis of the gene encoding 1-deoxy-d-xylulose 5-phosphate reductoisomerase from aquilaria sinensis (lour.) gilg.the major constituents of agarwood oils are sesquiterpenes that are obtained from isoprenoid precursors through the plastidial methylerythritol phosphate (mep) pathway and the cytosolic mevalonate pathway. in this study, a novel full-length cdna of 1-deoxy-d-xylulose 5-phosphate reductoisomerase (dxr), which was the second key enzyme in the plastid mep pathway of sesquiterpenes biosynthesis was isolated from the stem of aquilaria sinensis (lour.) gilg by the methods of reverse transcription poly ...201526174671
island cotton enhanced disease susceptibility 1 gene encoding a lipase-like protein plays a crucial role in response to verticillium dahliae by regulating the sa level and h2o2 accumulation.cotton is one of the most economically important crops, but most cultivated varieties lack adequate innate immunity or resistance to verticillium wilt. this results in serious losses to both yield and fiber quality. to identify the genetic resources for innate immunity and understand the pathways for pathogen defenses in this crop, here we focus on orthologs of the central arabidopsis thaliana defense regulator enhanced disease susceptibility 1 (eds1). the full-length cdna of gbeds1 was obtained ...201628018374
signaling pathways controlling induced resistance to insect herbivores in arabidopsis.insect attack triggers changes in transcript level in plants that are mediated predominantly by jasmonic acid (ja). the implication of ethylene (et), salicylic acid (sa), and other signals in this response is less understood and was monitored with a microarray containing insect- and defense-regulated genes. arabidopsis thaliana mutants coi1-1, ein2-1, and sid2-1 impaired in ja, et, and sa signaling pathways were challenged with the specialist small cabbage white (pieris rapae) and the generalist ...200717977152
antisense expression of gossypium barbadense ugd6 in arabidopsis thaliana significantly alters cell wall composition.uridine diphosphate-glucose dehydrogenase (ugd, ec1.1.1.22 oxidizes udp-glc (udp-d-glucose) to udp-glca (udp-d-glucuronate), a critical precursor of cell wall polysaccharides. gbugd6 from gossypium barbadense is more highly expressed late in the elongation of cotton fibers (15 d post-anthesis (dpa)) and during the stage of secondary cell wall thickening (30 dpa). subcellular localization analysis in onion epidermis revealed that fluorescently labeled gbugd6 protein was distributed throughout the ...201626810898
ethnobotanical study of medicinal plants by population of valley of juruena region, legal amazon, mato grosso, brazil.the use of medicinal plants for treatment, cure and prevention of diseases has been described by many people since time immemorial. because of this use, commercial and scientific interests have emerged, making it necessary to realize ethnobotanical surveys of medicinal plants species, which is important for subsequent chemical and pharmacological bioprospections.201526234177
a receptor-like kinase gene (gbrlk) from gossypium barbadense enhances salinity and drought-stress tolerance in arabidopsis.cotton (gossypium spp.) is widely cultivated due to the important economic value of its fiber. however, extreme environmental degradation impedes cotton growth and production. receptor-like kinase (rlk) proteins play important roles in signal transduction and participate in a diverse range of processes in response to plant hormones and environmental cues. here, we introduced an rlk gene (gbrlk) from cotton into arabidopsis and investigated its role in imparting abiotic stress tolerance.201323915077
overexpression of gbwrky1 positively regulates the pi starvation response by alteration of auxin sensitivity in arabidopsis.overexpression of a cotton defense-related gene gbwrky1 in arabidopsis resulted in modification of the root system by enhanced auxin sensitivity to positively regulate the pi starvation response. gbwrky1 was a cloned wrky transcription factor from gossypium barbadense, which was firstly identified as a defense-related gene and showed moderate similarity with atwrky75 from arabidopsis thaliana. overexpression of gbwrky1 in arabidopsis resulted in attenuated pi starvation stress symptoms, includin ...201222890372
photosynthetic electron transport in thylakoids from cotton cultivars (gossypium sp.) differing in tolerance to prometryn.a method to determine photosynthetic electron transport in thylakoid membranes is described for gossypium barbadense (cv. pima s-7) and g. hirsutum (cv. dp 5415). these cultivars differed markedly in tolerance to prometryn, a ps ii inhibitor. the rates of photosynthetic electron transport obtained were 245 μmole oxygen mg(-1) chl h(1). plant age and leaf size influenced the activity of the thylakoid preparations. thylakoids from leaves of plants 24 to 37 d and 50-70 mm in diameter had the highes ...199624178285
cotton defense induction patterns under spatially, temporally and quantitatively varying herbivory its defense against herbivores, cotton (gossypium sp.) relies in part on the production of a set of inducible, non-volatile terpenoids. under uniform damage levels, in planta allocation of induced cotton terpenoids has been found to be highest in youngest leaves, supporting assumptions of the optimal defense theory (odt) which predicts that plants allocate defense compounds to tissues depending on their value and the likelihood of herbivore attack. however, our knowledge is limited on how var ...201728270830
local and differential control of vegetative storage protein expression in response to herbivore damage in arabidopsis thaliana.vegetative storage proteins (vsps) are thought to fulfil important nutritional roles during plant development and stress adaptation. plant responses to mechanical wounding and herbivore damage include an activation of vsp expression. it was recently suggested that vsp is part of the systemic response of arabidopsis to wounding. to test this proposal, we monitored the spatial regulation of vsp mrnas and vsp proteins. arabidopsis contains two vsp genes and real-time quantitative pcr allowed us to ...200211982938
evidence for regulation of resistance in arabidopsis to egyptian cotton worm by salicylic and jasmonic acid signaling pathways.signaling cross-talk between wound- and pathogen-response pathways influences resistance of plants to insects and disease. to elucidate potential interactions between salicylic acid (sa) and jasmonic acid (ja) defense pathways, we exploited the availability of characterized mutants of arabidopsis thaliana (l.) heynh. and monitored resistance to egyptian cotton worm (spodoptera littoralis boisd.; lepidoptera: noctuidae). this generalist herbivore is sensitive to induced plant defense pathways and ...200211925049
genomic insights into divergence and dual domestication of cultivated allotetraploid cottons.cotton has been cultivated and used to make fabrics for at least 7000 years. two allotetraploid species of great commercial importance, gossypium hirsutum and gossypium barbadense, were domesticated after polyploidization and are cultivated worldwide. although the overall genetic diversity between these two cultivated species has been studied with limited accessions, their population structure and genetic variations remain largely unknown.201728219438
expression in pichia pastoris and characterization of two novel dirigent proteins for atropselective formation of gossypol.we established an efficient fed-batch fermentation process for two novel dirigent proteins from cotton plants, gbdir2 from gossypium barbadense and ghdir3 from g. hirsutum, using the engineered pichia pastoris glycoswitch® superman5 strain to prevent hyperglycosylation. the two (his)6-tagged proteins were purified by metal-chelate affinity chromatography and obtained in quantities of 12 and 15 mg l(-1) of culture volume, respectively. glycosylation sites were identified for the native and for th ...201727858135
quantitative trait loci analysis of verticillium wilt resistance in interspecific backcross populations of gossypium hirsutum × gossypium barbadense.verticillium wilt (vw) caused by verticillium dahliae (kleb) is one of the most destructive diseases of cotton. the identification of highly resistant qtls or genes in the whole cotton genome is quite important for developing a vw-resistant variety and for further molecular design breeding.201627814678
spatio-temporal, genotypic, and environmental effects on plant soluble protein and digestible carbohydrate content: implications for insect herbivores with cotton as an exemplar.plant soluble protein and digestible carbohydrate content significantly affect insect herbivore fitness, but studies reporting plant protein and carbohydrate content are rare. instead, the elements nitrogen and carbon often are used as surrogates for plant protein and digestible carbohydrate content, respectively. however, this is problematic for two reasons. first, carbon is found in all organic molecules, which precludes strong correlations with ecologically important dietary macronutrients (e ...201627738861
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