| adenine depurination and inactivation of plant ribosomes by an antiviral protein of mirabilis jalapa (map). | mirabilis antiviral protein (map) is a single-chain ribosome-inactivating protein (rip) isolated from mirabilis jalapa l. it depurinates the 28s-like rrnas of prokaryotes and eukaryotes. a specific modification in the 25s rrna of m. jalapa was found to occur during isolation of ribosomes by polyacrylamide/agarose composite gel electrophoresis. primer extension analysis revealed the modification site to be at the adenine residue corresponding to a4324 in rat 28s rrna. the amount of endogenous map ... | 1992 | 1463845 |
| functional analysis of a ring domain ankyrin repeat protein that is highly expressed during flower senescence. | a gene encoding a ring zinc finger ankyrin repeat protein (mjxb3), a putative e3 ubiquitin ligase, is highly expressed in petals of senescing four o'clock (mirabilis jalapa) flowers, increasing >40,000-fold during the onset of visible senescence. the gene has homologues in many other species, and the petunia homologue is strongly up-regulated in senescing petunia corollas. silencing the expression of this gene in petunia, using virus-induced gene silencing, resulted in a 2 d extension in flower ... | 2007 | 18057040 |
| molecular characterization and post-transcriptional regulation of me1, a type-i ribosome-inactivating protein from mirabilis expansa. | ribosome-inactivating proteins (rips) are n-glycosidases that remove a specific adenine from the sarcin/ricin (s/r) loop of the large rrna, thus arresting protein synthesis at the translocation step. in the present study, me1, a type-1 rip, was cloned and sequenced from storage roots of mirabilis expansa (ruiz & pavon). the full-length cdna sequence of me1 has 1,129 nucleotides with an open reading frame of 951 nucleotides representing 317 amino acids. nucleotide analysis revealed that the n-ter ... | 2003 | 12750888 |
| ribosome-inactivating and adenine polynucleotide glycosylase activities in mirabilis jalapa l. tissues. | several tissues of mirabilis jalapa l. (nyctaginaceae) were assayed for inhibition of translation by a rabbit reticulocyte lysate (as a signal of ribosome-inactivating activity) and for adenine dna glycosylase activity, activities that are both due to the presence of a class of enzymes called ribosome-inactivating proteins (rips), currently classified as rrna n-glycosylases (ec ). these activities were highest in seed; intermediate in flower bud, immature seed, sepal + gynoecium, leaf, and root; ... | 2002 | 11825901 |
| three new phenolic compounds from a manipulated plant cell culture, mirabilis jalapa. | bioassay-guided fractionation of an organic extract of the cell mass from a manipulated plant cell culture of mirabilis jalapa led to the isolation and subsequent identification of three new phenolic compounds, 1, 2, and 3. the isoflavone 1 and dehydrorotenoid 2 were identified as the principal antifungal principles from this plant cell culture with ic(50)'s of 25 and 48 microg/ml, respectively, against the test organism, candida albicans dsy1024. the rotenoid 3 was inactive at 200 microg/ml in ... | 2001 | 11277746 |
| mosquito larvicidal properties of mirabilis jalapa (nyctaginaceae) against anopheles stephensi, aedes aegypti & culex quinquefasciatus (diptera: culicidae). | | 0 | 25366214 |
| characterization of two novel type i ribosome-inactivating proteins from the storage roots of the andean crop mirabilis expansa. | two novel type i ribosome-inactivating proteins (rips) were found in the storage roots of mirabilis expansa, an underutilized andean root crop. the two rips, named me1 and me2, were purified to homogeneity by ammonium sulfate precipitation, cation-exchange perfusion chromatography, and c4 reverse-phase chromatography. the two proteins were found to be similar in size (27 and 27.5 kd) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and their isoelectric points were determined to be ... | 1999 | 10198104 |
| a new antifungal peptide from the seeds of phytolacca americana: characterization, amino acid sequence and cdna cloning. | an antifungal peptide from seeds of phytolacca americana, designated pafp-s, has been isolated. the peptide is highly basic and consists of 38 residues with three disulfide bridges. its molecular mass of 3929.0 was determined by mass spectrometry. the complete amino acid sequence was obtained from automated edman degradation, and cdna cloning was successfully performed by 3'-race. the deduced amino acid sequence of a partial cdna corresponded to the amino acid sequence from chemical sequencing. ... | 1999 | 10082954 |
| antimicrobial peptides from mirabilis jalapa and amaranthus caudatus: expression, processing, localization and biological activity in transgenic tobacco. | the cdnas encoding the seed antimicrobial peptides (amps) from mirabilis jalapa (mj-amp2) and amaranthus caudatus (ac-amp2) have previously been characterized and it was found that mj-amp2 and ac-amp2 are processed from a precursor preprotein and preproprotein, respectively [de bolle et al., plant mol biol 28:713-721 (1995) and 22:1187-1190 (1993), respectively]. in order to study the processing, sorting and biological activity of these antimicrobial peptides in transgenic tobacco, four differen ... | 1996 | 8843942 |
| dna sequence of mirabilis antiviral protein (map), a ribosome-inactivating protein with an antiviral property, from mirabilis jalapa l. and its expression in escherichia coli. | we cloned a cdna for mirabilis antiviral protein (map), a ribosome-inactivating protein (rip), which inhibits the mechanical transmission of plant virus and the in vitro protein synthesis of both prokaryotes and eukaryotes. the cdna consisted of 1066 nucleotides and could encode 278 amino acids. the major part of the amino acid sequence (from ala29 to ser278) was identical with the sequence of native map as determined by protein sequencing. an nh2-terminal extrapeptide (28 amino acid residues) o ... | 1991 | 2022657 |
| isolation and characterization of a novel class of plant antimicrobial peptides form mirabilis jalapa l. seeds. | we have isolated from seeds of mirabilis jalapa l. two antimicrobial peptides, designated mj-amp1 and mj-amp2, respectively. these peptides are highly basic and consist of 37 and 36 residues for mj-amp1 and mj-amp2, respectively. both peptides contain three disulfide bridges and differ from one another only by 4 amino acids. sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the reduced and unreduced peptides suggests that the peptides associate into dimers in their native for ... | 1992 | 1733929 |
| anti-inflammatory activity of aqueous extract of mirabilis jalapa linn. leaves. | theobjective of the present study was to evaluate the anti-inflammatory activity of aqueous extract of mirabilis jalapa linn. (mjl)(nyctaginaceae) leaves for scientific validation of the folklore claim of the plant. the leaves are used as traditional folk medicine in the south of brazil to treat inflammatory and painful diseases. cosmetic or dermo-pharmaceutical compositions containing mjl are claimed to be useful against inflammation and dry skin. | 2010 | 21713140 |
| inhibition of histamine mediated responses by mirabilis jalapa: confirming traditional claims made about antiallergic and antiasthmatic activity. | the roots of mirabilis jalapa are used traditionally in allergic skin disorders and asthma. the effect of an ethanol:acetone (1:1) extract of the roots of m. jalapa was studied for antihistaminic activity using a guinea pig tracheal chain preparation and clonidine-induced mast cell granulation in mice. its antiallergic activity was evaluated using milk-induced eosinophilia and albumin-induced paw oedema in mice. the extract (0.5 ml of 100 mg ml(-1)) inhibited histamine-induced guinea pig trachea ... | 2010 | 20981612 |
| bioluminescence imaging of clavibacter michiganensis subsp. michiganensis infection of tomato seeds and plants. | clavibacter michiganensis subsp. michiganensis is a gram-positive bacterium that causes wilting and cankers, leading to severe economic losses in commercial tomato production worldwide. the disease is transmitted from infected seeds to seedlings and mechanically from plant to plant during seedling production, grafting, pruning, and harvesting. because of the lack of tools for genetic manipulation, very little is known regarding the mechanisms of seed and seedling infection and movement of c. mic ... | 2010 | 20400561 |
| fibrinolytic serine protease isolation from bacillus amyloliquefaciens an6 grown on mirabilis jalapa tuber powders. | in this study, mirabilis jalapa tuber powder (mjtp) was used as a new complex organic substrate for the growth and production of fibrinolytic enzymes by a newly isolated bacillus amyloliquefaciens an6. maximum protease activity (1,057 u/ml) with casein as a substrate was obtained when the strain was grown in medium containing (grams per liter) mjtp 30, yeast extract 6, cacl(2) 1, k(2)hpo(4) 0.1, and k(2)hpo(4) 0.1. the strain was also found to grow and produce extracellular proteases in a medium ... | 2010 | 19842068 |
| detection of dopa 4,5-dioxygenase (dod) activity using recombinant protein prepared from escherichia coli cells harboring cdna encoding dod from mirabilis jalapa. | betalains are synthesized in flowers, fruits and other tissues of the plant order caryophyllales. betalamic acid is the chromophore of betalain pigments synthesized by a ring-cleaving enzyme reaction on l-dihydroxyphenylalanine (dopa). although reverse genetic evidence has proven that dopa 4,5-dioxygenase (dod) is a key enzyme of betalain biosynthesis, all attempts to detect recombinant plant dod activity in vitro have failed. here, we report on the formation of betalamic acid from dopa under su ... | 2009 | 19366710 |
| protein(s) from the gram-positive bacterium clavibacter michiganensis subsp. michiganensis induces a hypersensitive response in plants. | abstract the gram-positive tomato pathogen clavibacter michiganensis subsp. michiganensis induced a local necrotic response on four-o'clock (mirabilis jalapa) and tobacco (nicotiana tabacum) plants. this necrosis response was characteristic of the hypersensitive response (hr). the cell-free culture supernatant from strain cmm623 also induced a necrosis that was phenotypically similar to that induced by the bacteria. inhibitors of plant metabolism suppressed the necrotic reaction of both m. jalap ... | 1998 | 18944953 |
| antinociceptive activity of mirabilis jalapa in mice. | the infusion or decoction of mirabilis jalapa leaves is used in traditional medicine in brazil to treat inflammatory and painful diseases. | 2008 | 18761072 |
| optimization of alkaline protease production by aspergillus clavatus es1 in mirabilis jalapa tuber powder using statistical experimental design. | medium composition and culture conditions for the bleaching stable alkaline protease production by aspergillus clavatus es1 were optimized. two statistical methods were used. plackett-burman design was applied to find the key ingredients and conditions for the best yield. response surface methodology (rsm) including full factorial design was used to determine the optimal concentrations and conditions. results indicated that mirabilis jalapa tubers powder (mjtp), culture temperature, and initial ... | 2008 | 18481054 |
| [studies on chemical constituents from roots of mirabilis jalapa]. | to investigate the anti-hiv constituents from the root of mirabilis jalapa. | 2008 | 18338618 |
| pharmacological study of antispasmodic activity of mirabilis jalapa linn flowers. | mirabilis jalapa linn is a well-studied plant. the indigenous people of mexico use mirabilis jalapa to cure many infirmities including dysentery, diarrhea, muscular pain and abdominal colic. in the present investigation, we have further characterized some pharmacological properties of an extract of mirabilis jalapa flowers; therefore, we intend to contribute to understand the pharmacological effects and clarify the complex use of this medicinal plant. | 2008 | 18093770 |
| easy dna extraction for rapid detection of panax ginseng c. a. meyer in commercial ginseng products. | an investigation was carried out in order to obtain an easy and rapid detection of panax ginseng in commercial herbal products by using molecular techniques (polymerase chain reaction (pcr) and restriction fragment length polymorphism (rflp)). two protocols and one commercial kit for dna extraction were used. four forms of commercial products were considered, i.e., dried body roots, dried root tails, dried root prongs and dried extracts. the rflp of dna amplified products by 18df/28ccr primers, ... | 2007 | 17852746 |
| trypsin inhibitors from the garden four o'clock (mirabilis jalapa) and spinach (spinacia oleracea) seeds: isolation, characterization and chemical synthesis. | five serine proteinase inhibitors (mirabilis jalapa trypsin inhibitors, mjti i and ii and spinacia oleracea trypsin inhibitors, soti i, ii, and iii) from the garden four-o'clock (m. jalapa) and spinach (s. oleracea) seeds were isolated. the purification procedures included affinity chromatography on immobilized methylchymotrypsin in the presence of 5m nacl, ion exchange chromatography and/or preparative electrophoresis, and finally rp-hplc on a c-18 column. the inhibitors, crosslinked by three d ... | 2007 | 17481678 |
| n-caffeoylphenalkylamide derivatives as bacterial efflux pump inhibitors. | as part of an ongoing project to identify plant natural products as efflux pump inhibitors (epis), bioassay-guided fractionation of the methanolic extract of mirabilis jalapa linn. (nyctaginaceae) led to the isolation of an active polyphenolic amide: n-trans-feruloyl 4'-o-methyldopamine. this compound showed moderate activity as an epi against multidrug-resistant (mdr) staphylococcus aureus overexpressing the multidrug efflux transporter nora, causing an 8-fold reduction of norfloxacin mic at 29 ... | 2007 | 17275293 |
| enhanced resistance to early blight in transgenic tomato lines expressing heterologous plant defense genes. | genes coding for an iris ribosomal-inactivating protein (i-rip), a maize beta-glucanase (m-glu), and a mirabilis jalapa antimicrobial peptide (mj-amp1) were separately introduced into tomato (lycopersicon esculentum cv. sweet chelsea) cotyledons via agrobacterium tumefaciens-mediated transformation. transgenic lines carrying each of the transgenes were confirmed for integration into the tomato genome using southern blot hybridization. transcription of i-rip, m-glu, and mj-amp1 genes in various t ... | 2005 | 16047198 |
| improved crystals of the toxic protein map by protein engineering towards the host specificity. | mirabilis anti-viral protein (map) is a ribosome-inactivating protein from mirabilis jalapa l. since map is effective over a broad spectrum of species, the protein is difficult to express in heterologous hosts such as escherichia coli. recently, we obtained a map mutant, y72f which exhibits a lower (1/100) activity against e. coli ribosomes while retaining almost full activity against mammalian cells [habuka, miyano, kataoka, tsuge & noma (1992). j. biol. chem. 267, 7758-7760]. for the crystallo ... | 1994 | 15299393 |
| experimental diabetes treated with trigonelline: effect on β cell and pancreatic oxidative parameters. | oxidative stress in diabetes coexists with a reduction in the antioxidant status, which can further increase the deleterious effects of free radicals. trigonelline is the major component of mirabilis jalapa l., which has been used to treat diabetes in china. the present study was designed to evaluate the beneficial effects of trigonelline against hyperglycemia, hyperlipidemia, β cell damage and antioxidant of pancreas in diabetic rats. diabetic rats were induced by intraperitoneal injection 35 m ... | 2011 | 22172053 |
| mirabilis jalapa mottle virus: a new carlavirus infecting four o'clocks. | analysis of a next-generation sequence dataset from mirabilis jalapa resulted in the discovery of a novel virus in the genus carlavirus (family betaflexiviridae), mirabilis jalapa mottle virus (mjmv). the complete genome of mjmv was determined to consist of 8315 nucleotides (nt), with the six open reading frames indicative of carlaviruses. mjmv is most similar to kalanchoe latent virus (60% identity) and lily symptomless virus (59% identity). the virus can be transmitted mechanically to mirabili ... | 2011 | 21915718 |
| isolation and characterization of bioactive components from mirabilis jalapa l. radix. | the present investigation was carried out to isolate and characterize bioactive components from mirabilis jalapa l. radix ( zǐ mò lì gēn). thin-layer chromatography was used for the separation of spots from fractions of the crude extract. separated spots were collected for identification of their activities. free-radical scavenging activity was evaluated by spraying thin-layer chromatography plates (spotted with fractions) with 0.2% of 2,2-diphenyl-1-picrylhydrazyl solution. activity against hum ... | 2016 | 26870679 |
| molecular authentication of panax species. | using conserved plant sequences as primers, the dna sequences in the ribosomal its1-5.8s-its2 region have been amplified and determined for six panax species, p. ginseng c. a. mey. (oriental ginseng), p. quinquefolius l. (american ginseng), p. notoginseng (burkill) f. h. chen (sanchi), p. japonicus c. a. mey. (japanese ginseng), p. trifolius l. and p. major ting, as well as two common adulterants of ginseng, mirabilis jalapa l. and phytolacca acinosa roxb. an authentication procedure based upon ... | 1999 | 10192964 |
| the purification and amino acid sequences of four tx2 neurotoxins from the venom of the brazilian 'armed' spider phoneutria nigriventer (keys). | four neurotoxic polypeptides (tx2-1, txt2-5, tx2-6 and tx2-9) were purified from the venom of the south american 'armed' spider phoneutria nigriventer (keys) by gel filtration and reverse phase fplc and hplc. these cysteine-rich polypeptides exhibited different levels of neurotoxicity in mice after intracerebroventricular injection. tx2-1, tx2-5 and tx2-6 caused spastic paralysis and death, but the less toxic tx2-9 produced only tail erection and scratching. the molecular weights of the polypept ... | 1992 | 1397265 |
| kribbella mirabilis sp. nov., isolated from rhizosphere soil of a herbaceous plant, mirabilis jalapa l. | strain xmu 706(t), isolated from the rhizosphere soil of a herbaceous plant, mirabilis jalapa l., collected from xiamen city, china, was characterized using a polyphasic approach to clarify its taxonomic position. strain xmu 706(t) shared the highest 16s rrna gene sequence similarity with kribbella antibiotica yim 31530(t) (97.2%), and formed a distinct branch in the subclade of the genus kribbella in the 16s rrna gene phylogenetic tree. the genetic distances of gyrase subunit b gene (gyrb) sequ ... | 2015 | 26297172 |
| molecular characterization of a new begomovirus infecting mirabilis jalapa in northern india. | begomoviruses are whitefly-transmitted single-stranded dna viruses that are responsible for considerable economic losses. a begomovirus, alphasatellite and betasatellite were characterized in a mirabilis jalapa plant exhibiting severe leaf curling and mottling symptoms. the complete viral genome shared highest sequence identity of 87% with pedilanthus leaf curl virus (am712436), reported from pakistan. additionally, the viral genome was 84% identical to that of chilli leaf curl india virus (kx95 ... | 2017 | 28342034 |
| accumulation and function of trigonelline in non-leguminous plants. | as part of our studies of the occurrence, biosynthesis, function and human use of trigonelline, we looked at trigonelline-accumulating plant species and at the distribution of trigonelline in different organs of trigonelline-accumulating non-leguminous plants. there are many trigonelline-synthesizing plant species, but apart from legume seeds only a few species accumulate high concentrations of trigonelline. we have found only three species that accumulate high levels of trigonelline: murraya pa ... | 2014 | 25115081 |
| genes associated with opening and senescence of mirabilis jalapa flowers. | a modest ethylene climacteric accompanies flower senescence in mirabilis jalapa l., and exogenous ethylene accelerates the process. however, inhibitors of ethylene action and synthesis have little effect on the life-span of these ephemeral flowers. treatment with alpha-amanitin, an inhibitor of dna-dependent rna synthesis, substantially delays the onset of senescence. this effect falls linearly between 7 h and 8 h after the start of flower opening. subtractive hybridization was used to isolate t ... | 2007 | 17525082 |
| aspects of phytochrome decay in etiolated seedlings under continuous illumination. | the rate of total phytochrome decay in the dicotyledons amaranthus caudatus, mirabilis jalapa and pisum sativum under continuous illumination with red, incandescent, and blue light depends on the pfr/ptotal maintained by each source. amaranthus is an exception to this in that there is a deviation from firstorder decay kinetics under continuous illumination with incancdescent light. this deviation is probably not related to the chlorophyll present in the amaranthus sample since chlorophyll-rich p ... | 1972 | 24482270 |
| elucidation of the first committed step in betalain biosynthesis enables the heterologous engineering of betalain pigments in plants. | betalains are tyrosine-derived red-violet and yellow pigments, found in plants only of the caryophyllales order. although much progress has been made in recent years in the understanding of the betalain biosynthetic process, many questions remain open with regards to several of the proposed steps in the pathway. most conspicuous by its absence is the characterization of the first committed step in the pathway, namely the 3-hydroxylation of tyrosine to form l-3,4-dihydroxyphenylalanine (l-dopa). ... | 2016 | 26683006 |
| isolation, abundance and phylogenetic affiliation of endophytic actinomycetes associated with medicinal plants and screening for their in vitro antimicrobial biosynthetic potential. | microorganisms associated with medicinal plants are of interest as the producers of important bioactive compounds. to date, the diversity of culturable endophytic actinomycetes associated with medicinal plants is in its initial phase of exploration. in this study, 42 endophytic actinomycetes were isolated from different organs of seven selected medicinal plants. the highest number of isolates (n = 22, 52.3%) of actinomycetes was isolated from roots, followed by stems (n = 9, 21.4%), leaves (n = ... | 2015 | 25904906 |
| effector specialization in a lineage of the irish potato famine pathogen. | accelerated gene evolution is a hallmark of pathogen adaptation following a host jump. here, we describe the biochemical basis of adaptation and specialization of a plant pathogen effector after its colonization of a new host. orthologous protease inhibitor effectors from the irish potato famine pathogen, phytophthora infestans, and its sister species, phytophthora mirabilis, which is responsible for infection of mirabilis jalapa, are adapted to protease targets unique to their respective host p ... | 2014 | 24482481 |
| transposon-mediated mutation of cyp76ad3 affects betalain synthesis and produces variegated flowers in four o'clock (mirabilis jalapa). | the variegated flower colors of many plant species have been shown to result from the insertion or excision of transposable elements into genes that encode enzymes involved in anthocyanin synthesis. to date, however, it has not been established whether this phenomenon is responsible for the variegation produced by other pigments such as betalains. during betalain synthesis in red beet, the enzyme cyp76ad1 catalyzes the conversion of l-dihydroxyphenylalanine (dopa) to cyclo-dopa. rna sequencing ( ... | 2014 | 25151127 |
| structural characterization of spinacia oleracea trypsin inhibitor iii (soti-iii). | in recent decades, several canonical serine protease inhibitor families have been classified and characterized. in contrast to most trypsin inhibitors, those from garden four o'clock (mirabilis jalapa) and spinach (spinacia oleracea) do not share sequence similarity and have been proposed to form the new mirabilis serine protease inhibitor family. these 30-40-amino-acid inhibitors possess a defined disulfide-bridge topology and belong to the cystine-knot miniproteins (knottins). to date, no atom ... | 2013 | 23275169 |
| selection of plants for zinc and lead phytoremediation. | this article describes a laboratory scale experiment of phytoremediation that has the aim to identify a vegetable species that is able to survive to the high metal concentration of soil taken from montevecchio, sardinia, and verify how metal content in soil can influence phytoextraction performances. the experiments allowed finding a plant (festuca arundinacea) that is able to tolerate montevecchio soil and to accumulate high quantities of zinc in the shoots and to evaluate helichrysum italicum ... | 2004 | 15137716 |