| [effects of phytolacca acinosa polysaccharides i on cytotoxicity of macrophages and its production of tumor necrosis factor and interleukin 1]. | the in vivo effects of phytolacca acinosa polysaccharides i (pep-i) on immunologic cytotoxicity of mouse peritoneal macrophages and its production of tumor necrosis factor (tnf) and interleukin 1 (il-1) were studied. pep-i 80, 160 mg/kg was given ip twice every 4 d. both doses were found to have significant enhancing activity on macrophages cytotoxicity against s180 sarcoma cells and malignant transformed fibroblast l929 cells. peritoneal activated macrophages were incubated with lps for 2 and 2 ... | 1990 | 2104493 |
| antitumor activity and tumor necrosis factor production of phytolacca acinosa polysaccharides i in mice. | the antitumor activities of phytolacca acinosa polysaccharides i (pap-i) and its effects on the induction of tumor necrosis factor (tnf) and immunological cytotoxicity of peritoneal macrophages were studied. pap-i was given ip 5-20 mg.kg-1.d-1 x 7 d to icr mice as priming agent with subsequent lipopolysaccharides (10 micrograms/mouse) iv for tnf production. tnf activity was measured by crystal violet staining assay using l929 cells. pap-i showed priming activity for tnf production with hepto-spl ... | 1993 | 8010055 |
| [effect of phytolacca acinosa polysaccharides i on production of colony-stimulating factors of mouse splenocytes in vitro]. | phytolacca acinose polysaccharides i (pap-i), m(r) = 10,000, activated mouse splenocytes to produce colony-stimulating factors (csf) in vitro. the level of csf was tested by [3h]tdr uptaken by bone marrow cells and rmgm-csf was used as standard. pap-i (10-500 micrograms.ml-1) increased csf production of the splenocytes treated with or without concanavalin a. when the concentration of pap-i was 100 micrograms.ml-1, the level of csf was about equivalent to that of rmgm-csf 11.8 +/- 1.8 ng.ml-1. af ... | 1993 | 8010070 |
| molecular authentication of panax species. | using conserved plant sequences as primers, the dna sequences in the ribosomal its1-5.8s-its2 region have been amplified and determined for six panax species, p. ginseng c. a. mey. (oriental ginseng), p. quinquefolius l. (american ginseng), p. notoginseng (burkill) f. h. chen (sanchi), p. japonicus c. a. mey. (japanese ginseng), p. trifolius l. and p. major ting, as well as two common adulterants of ginseng, mirabilis jalapa l. and phytolacca acinosa roxb. an authentication procedure based upon ... | 1999 | 10192964 |
| acinospesigenin-a, -b, and -c: three new triterpenoids from phytolacca acinosa. | three new triterpenoids, designated as acinospesigenin-a (1), -b (2), and -c (3), isolated from the berries of phytolacca acinosa, have been characterized as 3 beta-acetoxy-11 alpha,23-dihydroxytaraxer-14-en-28-oic acid, olean-12-en-23-al-2 beta,3 beta-dihydroxy-30-methoxycarbonyl-28-oic acid and olean-12-en-23-al-2 beta,3 beta,11 alpha-trihydroxy-30-methoxycarbonyl-28-oic acid, respectively. the compounds have shown antiedemic activity (ld(50) 10-15 mg/kg mass) in albino rats. | 2003 | 12932140 |
| effects of aqueous extracts of aconitum carmichaeli, rhizoma bolbostemmatis, phytolacca acinosa, panax notoginseng and gekko swinhonis gūenther on bel-7402 cells. | to investigate the anticancer activity of a chinese medical mixture, wrcp (warming and relieving cold phlegm), on hepatocarcinoma bel-7402 cells. | 2007 | 17569146 |
| easy dna extraction for rapid detection of panax ginseng c. a. meyer in commercial ginseng products. | an investigation was carried out in order to obtain an easy and rapid detection of panax ginseng in commercial herbal products by using molecular techniques (polymerase chain reaction (pcr) and restriction fragment length polymorphism (rflp)). two protocols and one commercial kit for dna extraction were used. four forms of commercial products were considered, i.e., dried body roots, dried root tails, dried root prongs and dried extracts. the rflp of dna amplified products by 18df/28ccr primers, ... | 2007 | 17852746 |
| functional characterization of a potassium transporter gene nrhak1 in nicotiana rustica. | the purpose of this study is to investigate the function of a novel potassium transporter gene (nrhak1) isolated from nicotiana rustica roots using yeast complement and real-time pcr technique. the complementary dna (cdna) of nrhak1, 2 488 bp long, contains an open reading frame (orf) of 2 334 bp encoding a protein of 777 amino acids (87.6 kda) with 12 predicted transmembrane domains. the nrhak1 protein shows a high sequence similarity to those of high-affinity potassium transporters in mesembry ... | 2008 | 19067462 |
| screening of molluscicidal strain against oncomelania hupensis from the rhizosphere of medicinal plant phytolacca acinosa roxb. | the research aimed to screen and exploit molluscicidal microorganisms against oncomelania hupensis, from the rhizosphere of medicinal plant, phytolacca acinosa roxb., and one strain named as sl-30 was obtained with excellent molluscicidal activity. the freeze-dried powder of exocellular broth (exb) of sl-30 could kill 100% of snails at a concentration of 48 mg/l for a submerged period of 24 h, with stabile molluscicidal activity at a temperature lower than 60°c; furthermore, it could be graduall ... | 2010 | 20931072 |
| endophyte-assisted promotion of biomass production and metal-uptake of energy crop sweet sorghum by plant-growth-promoting endophyte bacillus sp. sls18. | the effects of bacillus sp. sls18, a plant-growth-promoting endophyte, on the biomass production and mn/cd uptake of sweet sorghum (sorghum bicolor l.), phytolacca acinosa roxb., and solanum nigrum l. were investigated. sls18 displayed multiple heavy metals and antibiotics resistances. the strain also exhibited the capacity of producing indole-3-acetic acid, siderophores, and 1-aminocyclopropane-1-carboxylic acid deaminase. in pot experiments, sls18 could not only infect plants effectively but a ... | 2011 | 21792590 |
| Anticancer effects of 5-fluorouracil combined with warming and relieving cold phlegm formula on human breast cancer. | OBJECTIVE: To investigate the anticancer effects of warming and relieving cold phlegm formula (, WRCP), a Chinese medical mixture composed of the aqueous extracts of Aconitum carmichaeli, Rhizoma bolbostemmatis, Phytolacca acinosa, Panax notoginseng, and Gekko swinhonis G u enther, combined with 5-fluorouracil (5-FU) on human breast cancer in vivo. METHODS: Seventy-two Nu/Nu mice inoculated with MDA-MB-231 breast ... | 2011 | 22160948 |
| solirubrobacter taibaiensis sp. nov., isolated from a stem of phytolacca acinosa roxb. | a white-coloured bacterium, designated strain gtjr-20(t), was isolated from a stem of phytolacca acinosa roxb. collected from taibai mountain in shaanxi province, north-west china, and was subjected to a taxonomic study by using a polyphasic approach. the novel isolate was found to grow optimally at 28-30 °c, at ph 7.5-8.0 and in the absence of nacl. cells were observed to be gram-stain positive, strictly aerobic, rod-shaped and non-motile. the predominant respiratory quinone was identified as m ... | 2014 | 24846053 |
| solirubrobacter phytolaccae sp. nov., an endophytic bacterium isolated from roots of phytolacca acinosa roxb. | a gram-staining-positive, strictly aerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated gtgr-8(t), which formed white colonies, was isolated from roots of phytolacca acinosa roxb. collected from taibai mountain in shaanxi province, north-west china. strain gtgr-8(t) grew optimally at 28-30 °c, at ph 7.0-8.0 and in the absence of nacl. phylogenetic analyses based on 16s rrna gene sequences showed that strain gtgr-8(t) was a member of the genus solirubrobacter and was cl ... | 2014 | 24254741 |
| [biological safety of the molluscicidal ingredient from aspergillus fumigatus sl-30 isolated from rhizosphere of phytolacca acinosa]. | to study biological safety of the molluscicidal ingredient (mi, a kind of diketopiperazines) in the exocellular broth of aspergillus fumigatus sl-30' which was isolated from the rhizosphere of phytolacca acinosa. | 2012 | 23484258 |