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ultrastructural and immunological demonstration of the nodulation of the european alnus glutinosa (l.) gaertn. host plant by the north-american alnus crispa var. mollis fern. root nodule endophyte.the inoculation of the european alnus glutinosa (l.) gaertn. host plant by a crushed-nodule inoculum, prepared with the north-american alnus crispa var. mollis fern. root nodule, was successful. fluorescein- and ferritin-labelled antibodies, specific against the a. crispa var. mollis root nodule endophyte (lalonde et al. 1975), demonstrated the idenity of this endophyte in the resulting nodules. the nodulation process of this abnormal host-endophyte system was studied by light and electron micro ...1977922604
the nh2-terminal amino acid sequence of the immunochemically partial identical major allergens of alder (alnus glutinosa) aln g i, birch (betula verrucosa) bet v i, hornbeam (carpinus betulus) car b i and oak (quercus alba) que a i pollens.the tree pollen major allergens (aln g i), bet v i, car b i and que a i, were purified to near homogeneity. rabbit antibodies raised towards the major allergen bet v i react with the major allergens of alder, hazel, hornbeam and oak, although with distinct variation in affinity. immunochemically the major allergens seem to resemble one another more within than between the botanically established families of the fagale order. the physico-chemical parameters (molecular weight, pi values, amino aci ...19911961201
immunochemical characterization of reference alder (alnus glutinosa) and hazel (corylus avellana) pollen extracts and the partial immunochemical identity between the major allergens of alder, birch and hazel pollens.dialysed extracts of alder (alnus glutinosa) and hazel (corylus avellana) pollens were characterized by immunochemical methods. the alder pollen extract contained at least 33 distinct antigens of which one, antigen 5, was classified as a major allergen, three, antigens 4, 10, and 17, were classified as intermediate allergens and two, antigens 19 and 23, were classified as minor allergens. the molecular weight and pi of the major allergen were determined to be 19,000 d and 5.2, respectively. the ...19854073410
[pharmacological study of alnus glutinosa gaertn. bark]. 19714259490
[electron microscopic study of the developmental cycle of an actinomycete endosymbiont in the nitrogen-fixing nodules on the roots of alnus glutinosa].as has been shown by electron microscopy, the development of the endosymbiont in the nitrogen-fixing nodules of a. glutinosa proceeds either along the known (hyphae leads to vesicles) or less convenient pattern (hyphae leads to sporangia leads to spores). the paper presents more complete information about the structure of layers which separate the endosymbiont from the cytoplasm of host cells and about changes in the structure of the both partners accompanying sporogenesis.19807412621
phylogenetic studies on uncultured frankia populations in nodules of datisca cannabina.part of the 16s rrna gene was amplified directly from uncultured endophyte populations within the root nodules of datisca cannabina and three strains isolated from nodules of alnus glutinosa (agkg'84/4), coriaria nepalensis (cn3), and d. cannabina (dc2). sequence comparison based on 930 nucleotides indicated that the endophyte of d. cannabina nodules belongs to the genus frankia and is highly related to the endophyte of c. nepalensis root nodules. the relatedness of the endophytes of c. nepalens ...19947518733
differential gene expression in an actinorhizal symbiosis: evidence for a nodule-specific cysteine proteinase.nodules formed on the roots of actinorhizal plants as a consequence of nitrogen-fixing symbioses with the actinomycete frankia appear to result from modification of the developmental pathway that leads to lateral root formation. presently no information exists about factors that control this developmental switch or, until now, about genes that are differentially expressed as a result of an altered developmental pathway. differential screening of an alnus glutinosa nodule cdna library revealed al ...19947937912
sucrose synthase and enolase expression in actinorhizal nodules of alnus glutinosa: comparison with legume nodules.two different types of nitrogen-fixing root nodules are known- actinorhizal nodules induced by frankia and legume nodules induced by rhizobia. while legume nodules show a stem-like structure with peripheral vascular bundles, actinorhizal nodule lobes resemble modified lateral roots with a central vascular bundle. to compare carbon metabolism in legume and actinorhizal nodules, sucrose synthase and enolase cdna clones were isolated from a cdna library, obtained from actinorhizal nodules of alnus ...19968602161
high-level expression of tree pollen isoallergens in escherichia coli.cdnas coding for the major allergen of alder (alnus glutinosa) pollen aln g 1, for nine isoforms of bet v 1, the major birch (betula verrucosa) pollen allergen, and for four isoforms of cor a 1, the major allergen of hazel (corylus avellana) pollen, were inserted into the plasmid pmw175 or pmw 172 and expressed in escherichia coli as recombinant non-fusion proteins. these constructs produced between 20 and 160 mg protein/l. the recombinant tree pollen isoallergens were tested in immunoblots for ...19968688676
a nodule-specific gene family from alnus glutinosa encodes glycine- and histidine-rich proteins expressed in the early stages of actinorhizal nodule development.two cdnas representing different members (agnt84 and ag164) of a gene family encoding glycine- and histidine-rich proteins have been isolated from cdna libraries from alnus glutinosa root nodules. expression of the corresponding genes could only be detected in nodules. with in situ hybridization, the expression in nodules was found to occur in young, infected cells of the prefixation zone (zone 2). the encoded proteins contain putative signal peptides for targeting to the endomembrane system, sh ...19979204569
quantification in mass units of bet v 1, the main allergen of betula verrucosa pollen, by a monoclonal antibody based-elisa.fagales pollens are considered among the main agents responsible for allergic diseases in many countries of the northern hemisphere and single major allergens have been shown to be responsible for these responses.19979291291
differential toxicity of leaf litter to dipteran larvae of mosquito developmental sites.the relative toxicity of leaf litter to nematocerous dipteran larvae characteristic of mosquito developmental sites was investigated. culicidae, chironomidae, and simuliidae taxa originating from alpine hydrosystems were tested together with two laboratory nonindigenous culicid taxa. bioassays indicate that ingestion of 10-month-old decaying leaves from alnus glutinosa, populus nigra, and quercus robur by larvae is more deleterious for aedes aegypti, a. albopictus, culex pipiens, simulium varieg ...200010631052
the involvement of a cysteine proteinase in the nodule development in chinese milk vetch infected with mesorhizobium huakuii subsp. rengei.cys proteinases play important roles in plant cell development and senescence. a cdna, asnodf32, obtained by differential screening of a nodule cdna library of the leguminous plant chinese milk vetch (astragalus sinicus), represents a nodule-specific cys proteinase similar to that reported for the actinorhizal alnus glutinosa-flankia symbiosis. a characteristic feature of this proteinase is the presence of a putative vacuolar targetting signal, lqda, within its propeptide. expression of the asno ...200011080286
effect of inoculation and leaf litter amendment on establishment of nodule-forming frankia populations in soil.high-n(2)-fixing activities of frankia populations in root nodules on alnus glutinosa improve growth performance of the host plant. therefore, the establishment of active, nodule-forming populations of frankia in soil is desirable. in this study, we inoculated frankia strains of alnus host infection groups i, iiia, and iv into soil already harboring indigenous populations of infection groups (iiia, iiib, and iv). then we amended parts of the inoculated soil with leaf litter of a. glutinosa and k ...200111375169
modification of the protein expression pattern induced in the nitrogen-fixing actinomycete frankia sp. strain acn14a-tsr by root exudates of its symbiotic host alnus glutinosa and cloning of the sodf gene.two-dimensional (2-d) polyacrylamide gel electrophoresis was used to detect proteins induced in frankia sp. strain acn14a-tsr by root exudates of its symbiotic host, alnus glutinosa. the 5 most prominent proteins were purified from 2-d gels and characterized by n-terminal sequencing. all of these proteins had a high percentage of similarity with known stress proteins. one protein match was the fe superoxide dismutase (fe-sod), another was a tellurite resistance protein (ter), the third was a bac ...200111467730
phosphoenolpyruvate carboxylase in mistletoe leaves: regulation of gene expression, protein content, and covalent modification.seasonal changes in the activity of phosphoenolpyruvate carboxylase (pepcase, ec 4.1.1.31), a key enzyme in the interaction of carbohydrate and nitrogen metabolism, were studied in leaves of the c3 semiparasitic mistletoe, viscum album, growing on different host trees. maximum extractable pepcase activities were higher in leaves of mistletoes growing on betula pendula and alnus glutinosa hosts compared with those on the conifers, abies alba and larix decidua. independent of host, maximum extract ...200111473691
effects of rooting and tree growth of selected woodland species on cap integrity in a mineral capped landfill site.the above and below ground growth of three tree species (alnus glutinosa, pinus nigra var. maritima and acer pseudoplatanus) was studied on a containment landfill site at waterford, hertfordshire, uk. tree root architecture was studied using soil inspection pits excavated next to 12 trees of each species and mapped in detail. tree height was related to soil thickness over the compacted mineral cap. no roots entered the cap where soil thickness was 1.3 m, but a few roots, especially of alder, wer ...200111699854
use of a monoclonal antibody-based immunoassay for the detection and quantification of heliscus lugdunensis colonizing alder leaves and roots.a genus-specific monoclonal antibody, ng-cf10, raised in a previous study to the fungal pathogen nectria galligena, was found to recognize the aquatic hyphomycete heliscus lugdunensis (anamorph) and its teleomorph nectria lugdunensis. using this mab in a plate trapped antigen- elisa we could detect and determine the biomass of heliscus lugdunensis in mixed assemblages in both naturally occurring and artificially inoculated leaves and roots of alnus glutinosa trees. initial studies indicate that ...200112024233
ectomycorrhizal root development in wet alder carr forests in response to desiccation and eutrophication.effects of desiccation and eutrophication on ectomycorrhizal (ecm) root development in wet alder carr forests in the netherlands were studied. in northwestern europe, wet alder carr forests are found mostly in peatlands and along streams, forming an important component of wetland ecosystems. the dominant tree species in wet alder carr forests is alnus glutinosa (l.) gaertn. (black alder), which associates with ectomycorrhizal fungi. during recent decades, wet alder carr forests in europe have de ...200212072985
spatial analysis of plant detritus processing in a mediterranean river type: the case of the river tirso basin, sardinia, italy.the river continuum concept represents the most general framework addressing the spatial variation of both structure and function in river ecosystems. in the mediterranean ecoregion, summer drought events and dams constitute the main sources of local disturbance to the structure and functioning of river ecosystems occurring in the river basin. in this study, we analysed patterns of spatial variation of detritus processing in a 7th order river of the mediterranean ecoregion (river tirso, sardinia ...200312765266
phytophthora pseudosyringae sp. nov., a new species causing root and collar rot of deciduous tree species in europe.in several studies of oak decline in europe, a semi-papillate homothallic phytophthora taxon was consistently isolated, together with other phytophthora species, from rhizosphere soil samples. it was also found associated with necrotic fine roots and stem necroses of fagus sylvatica and alnus glutinosa. due to morphological and physiological similarities, the semi-papillate isolates were previously identified as p. syringae by various authors. the morphology, physiology and pathogenicity against ...200312967204
distinct patterns of symbiosis-related gene expression in actinorhizal nodules from different plant families.phylogenetic analyses suggest that, among the members of the eurosid i clade, nitrogen-fixing root nodule symbioses developed multiple times independently, four times with rhizobia and four times with the genus frankia. in order to understand the degree of similarity between symbiotic systems of different phylogenetic subgroups, gene expression patterns were analyzed in root nodules of datisca glomerata and compared with those in nodules of another actinorhizal plant, alnus glutinosa, and with t ...200312971603
electron microscopy of the endophyte of alnus glutinosa. 196414274130
[polyclonal antisera to birch pollen allergen for the air allergen screening].the cross reactivity of the pollen allergens of birch (betula pendula), hazel (corylus avelana), alder (alnus glutinosa), wormwood (artemisia absinthium), oak (quercus robur) and ashtree (fraxinus excelsior) was studied by the methods of the enzyme immunoassay and gel diffusion with the use of rabbit polyclonal antisera (pas) prepared against birch pollen allergen. pas was shown to be greatly related to all above-mentioned allergens with the exception of oak pollen allergen. pas was found capabl ...200415024987
novel expression pattern of cytosolic gln synthetase in nitrogen-fixing root nodules of the actinorhizal host, datisca glomerata.gln synthetase (gs) is the key enzyme of primary ammonia assimilation in nitrogen-fixing root nodules of legumes and actinorhizal (frankia-nodulated) plants. in root nodules of datisca glomerata (datiscaceae), transcripts hybridizing to a conserved coding region of the abundant nodule isoform, dggs1-1, are abundant in uninfected nodule cortical tissue, but expression was not detectable in the infected zone or in the nodule meristem. similarly, the gs holoprotein is immunolocalized exclusively to ...200415247391
meteorological factors and air pollution in lithuanian forests: possible effects on tree condition.this study investigates changes in tree condition and environmental factors in lithuania during the active growing season in 1991-2001. the average crown defoliation and the proportion of healthy trees of pinus sylvestris, picea abies, betula sp., fraxinus excelsior, alnus incana, alnus glutinosa, populus tremula, and quercus robur, meteorological (average temperature, amount of precipitation, hydrothermal coefficient) and air pollution data (acidity of precipitation, concentrations of so2, no2 ...200516005769
synergistic effect of glomus intraradices and frankia spp. on the growth and stress recovery of alnus glutinosa in an alkaline anthropogenic sediment.the presence of actinorhizas and arbuscular mycorrhizas may reduce plant stresses caused by adverse soil conditions. a greenhouse experiment was conducted using a sediment with a high ph, resulting from the disposal of waste originated at an acetylene and polyvinylchloride factory, in which black alder (alnus glutinosa) seedlings were inoculated either with glomus intraradices beg163 (originally isolated from the same sediment), frankia spp. or both symbionts. after a 6-month growth period, plan ...200516054916
oligonucleotide probes that hybridize with rrna as a tool to study frankia strains in root nodules.oligonucleotide probes that hybridize with specific sequences in variable regions of the 16s rrna of the nitrogen-fixing actinomycete frankia were used for the identification of frankia strains in nodules. frankia cells were released from plant tissue by grinding glutaraldehyde-fixed root nodules in guanidine hydrochloride solution. rrna was obtained after sonication, precipitation with ethanol, and purification by phenolchloroform extraction. degradation of rrna, evident in northern blots, did ...199016348187
clinical efficacy and safety of sublingual immunotherapy with tree pollen extract in children.subcutaneous immunotherapy has been the principal approach of immunotherapy in the treatment of allergic diseases. several clinical studies with birch, alder or hazel pollen extract conducted as subcutaneous immunotherapy have been published suggesting a well-tolerated and clinically effective treatment. only a few clinical studies of sublingual immunotherapy (slit) with these allergens have been published. this study investigated the clinical efficacy, safety and dose-response relationship of s ...200616942565
sublingual immunotherapy in children modulates allergen-induced in vitro expression of cytokine mrna in pbmc.during subcutaneous immunotherapy (scit), there is a local mucosal shift from th2 to th1 type cytokine predominance and downregulation of interleukin (il)-5 and eosinophilia. according to recent studies il-10- and transforming growth factor (tgf)-beta-induced tolerance is another key phenomenon in scit. few data to date is available on mechanisms and roles of these cytokines in sublingual immunotherapy (slit).200616942566
daldinia decipiens and entonaema cinnabarina as fungal symbionts of xiphydria wood wasps.the identity of symbiotic fungi associated with the xiphydria spp. wood wasps was investigated using dna analysis. the fungi were isolated from the mycangia of adult females of x. camelus, x. prolongata and x. longicollis reared from colonized logs of alnus glutinosa, salix alba and quercus robur, respectively. sequences of rdna and beta-tubulin were obtained. phylogenetic analysis based on the nj method showed that the isolates from x. camelus clustered with daldinia decipiens, whereas those of ...200717188483
comparison of 226ra nuclide from soil by three woody species betula pendula, sambucus nigra and alnus glutinosa during the vegetation period.the uptake of 226ra from the contaminated soil was compared in three woody species: alder (alnus glutinosa), birch (betula pendula) and elder (sambucus nigra). the 226ra activities increased during the vegetation periods (in 2003, 2004 and 2005) both in the leaves and flowers+seeds. the highest accumulation was found in birch, reaching 0.41 bq/g dw in the leaves (at the end of the vegetation period in 2003). the lowest 226ra accumulation was determined in alder. the extent of 226ra accumulation ...200717467859
cell-free nitrogenase and hydrogenase from actinorhizal root nodules.field-grown alder (alnus glutinosa) root nodules were disrupted in liquid nitrogen to release the actinomycete endophytes. the endophytes were broken by mild sonic oscillation and yielded a cell-free nitrogenase preparation capable of reducing acetylene and protons. in addition, the preparation carried a cell-free uptake hydrogenase.197917781259
phytophthora gallica sp. nov., a new species from rhizosphere soil of declining oak and reed stands in france and germany.a non-papillate, slow-growing phytophthora species, which could not be assigned to any existing taxon, was isolated from rhizosphere soil of a declining oak in northeast france, and from the rhizosphere of phragmites australis at lake constance in south-west germany in 1998 and 2004, respectively. we describe this species, previously informally designated phytophthora taxon 'g', as phytophthora gallica sp. nov. morphology, growth rates, and pathogenicity against cuttings of riparian tree species ...200818693002
antifeedants and feeding stimulants in bark extracts of ten woody non-host species of the pine weevil, hylobius abietis.bark of ten woody species, known to be rejected as a food source by the pine weevil, hylobius abietis, were sequentially extracted by a soxhlet apparatus with pentane followed by methanol. species were alder (alnus glutinosa), aspen (populus tremula), beech (fagus sylvatica), guelder rose (viburnum opulus), holly (ilex aquifolium), horse chestnut (aesculus hippocastanum), lilac (syringa vulgaris), spindle tree (evonymus europaeus), walnut (juglans regia), and yew (taxus baccata). bark of each sp ...200818719963
range of phytoplasma concentrations in various plant hosts as determined by competitive polymerase chain reaction.abstract for competitive polymerase chain reaction (pcr), an internal standard dna template was developed that consisted of a highly conserved, internally deleted 16s rdna fragment of an aster yellows phytoplasma. the internal standard was calibrated using a quantified culture of acholeplasma laidlawii. serial dilutions of the internal standard and fixed amounts of target templates from infected plants were coamplified with the same primers, and the products obtained were quantified using an enz ...200018944479
phytophthora polonica, a new species isolated from declining alnus glutinosa stands in poland.in a survey of phytophthora associated with alder decline in poland, several isolates of a homothallic phytophthora sp., which could not be assigned to other taxa including phytophthora alni subspecies, were consistently recovered from rhizosphere soil samples. their morphology and pathogenicity, as well as sequence data for three nuclear regions (internal transcribed spacer rdna, elongation factor-1alpha and beta-tubulin) and a coding mitochondrial dna region (nadh1), were examined. the new phy ...200616907716
[sensitization to castanea sativa pollen and pollinosis in northern extremadura (spain)].castanea sativa pollen allergy has generally been considered to be uncommon and clinically insignificant. in our geographical area (plasencia, cáceres, spain) castanea sativa pollen is a major pollen.200515946627
in vitro anticancer potential of tree extracts from the walloon region forest.forty-eight extracts from 16 common belgian trees from the walloon region forest were evaluated for in vitro growth inhibitory activity against the human lovo colon cancer, pc3 prostate cancer, and u373 glioblastoma cell lines. our study was performed with the aim of selecting plant candidates in order to later isolate new anticancer compounds from an easily affordable tree material. extracts from alnus glutinosa (stem bark), carpinus betulus (leaves and stem bark), castanea sativa (stem bark), ...200919579185
a nodule-specific dicarboxylate transporter from alder is a member of the peptide transporter family.alder (alnus glutinosa) and more than 200 angiosperms that encompass 24 genera are collectively called actinorhizal plants. these plants form a symbiotic relationship with the nitrogen-fixing actinomycete frankia strain hfpari3. the plants provide the bacteria with carbon sources in exchange for fixed nitrogen, but this metabolite exchange in actinorhizal nodules has not been well defined. we isolated an alder cdna from a nodule cdna library by differential screening with nodule versus root cdna ...200415001700
autumnal changes of sulfur fractions and the ratio of organic sulfur to total nitrogen in leaves and adjacent bark of eastern cottonwood, white basswood and actinorhizal black alder.autumnal changes in organic-s, sulfate-s, total-s and the ratios of organic-s to total-n and sulfate-s to organic-s were followed in leaves and adjacent bark of actinorhizal (frankia-nodulated) black alder (alnus glutinosa (l.) gaertn.) and eastern cottonwood (populus deltoides bartr. ex marsh.) trees growing on a minespoil site high in extractable soil sulfate, and in black alder and white basswood (tilia heterophylla venten.) trees growing on a prairie-derived soil in illinois. organic-s conce ...198814972822
high autumn temperature delays spring bud burst in boreal trees, counterbalancing the effect of climatic warming.the effect of temperature during short-day (sd) dormancy induction was examined in three boreal tree species in a controlled environment. saplings of betula pendula roth, b. pubescens ehrh. and alnus glutinosa (l.) moench. were exposed to 5 weeks of 10-h sd induction at 9, 15 and 21 degrees c followed by chilling at 5 degrees c for 40, 70, 100 and 130 days and subsequent forcing at 15 degrees c in a 24-h photoperiod for 60 days. in all species and with all chilling periods, high temperature duri ...200314532017
effects of elevated carbon dioxide concentration on growth and nitrogen fixation in alnus glutinosa in a long-term field experiment.nitrogen-fixing plant species may respond more positively to elevated atmospheric carbon dioxide concentrations ([co2]) than other species because of their ability to maintain a high internal nutrient supply. a key factor in the growth response of trees to elevated [co2] is the availability of nitrogen, although how elevated [co2] influences the rate of n2-fixation of nodulated trees growing under field conditions is unclear. to elucidate this relationship, we measured total biomass, relative gr ...200312975129
characterization of the sodf gene region of frankia sp. strain acn14a and complementation of escherichia coli sod mutant.the frankia sp. strain acn14a superoxide dismutase sodf was previously shown to be induced in response to alnus glutinosa root exudates, and its gene was sequenced. we report here the sequence of the 9-kb genomic segment surrounding the sodf gene and further characterize this gene and its product. nine orfs coding for various proteins, such as regulators, acetyl-coa transferases, and a bacterioferritin a next to the sodf gene, were found. northern blot analysis showed that the sodf gene was expr ...200312897839
mosquito larval consumption of toxic arborescent leaf-litter, and its biocontrol potential.previously we described the mosquito larvicidal properties of decomposed leaf-litter from deciduous trees, especially the alder alnus glutinosa (l) gaertn., due to toxic polyphenols and other secondary compounds. to further examine the biocontrol potential of toxic leaf-litter for mosquito control, feeding rates of third-instar mosquito larvae were assessed for examples of three genera: anopheles stephensi liston, aedes aegypti (l) and culex pipiens l. (diptera: culicidae). when immersed in a su ...200312823832
comparison of planted soil infiltration systems for treatment of log yard runoff.treatment of log yard runoff is required to avoid contamination of receiving watercourses. the research aim was to assess if infiltration of log yard runoff through planted soil systems is successful and if different plant species affect the treatment performance at a field-scale experimental site in sweden (2005 to 2007). contaminated runoff from the log yard of a sawmill was infiltrated through soil planted with alnus glutinosa (l.) gärtner (common alder), salix schwerinii x viminalis (willow ...201020669729
aquatic hyphomycetes in polluted groundwater habitats of central germany.polluted groundwater wells located in a former copper shale mining district (11 sites; mansfelder land, central germany) and in meadows of the mulde and elbe rivers (2 sites) were assessed for occurrence and species richness of aquatic hyphomycetes. water temperatures at all sites were relatively low and fluctuated less than in surface waters. oxygen concentrations were always below saturation, whereas sulfate, nitrate, and phosphate levels reached extremely high values in several of the wells. ...200312704555
transcriptomics of actinorhizal symbioses reveals homologs of the whole common symbiotic signaling cascade.comparative transcriptomics of two actinorhizal symbiotic plants, casuarina glauca and alnus glutinosa, was used to gain insight into their symbiotic programs triggered following contact with the nitrogen-fixing actinobacterium frankia. approximately 14,000 unigenes were recovered in roots and 3-week-old nodules of each of the two species. a transcriptomic array was designed to monitor changes in expression levels between roots and nodules, enabling the identification of up- and down-regulated g ...201121464474
the range of ixodes ricinus and the risk of contracting lyme borreliosis will increase northwards when the vegetation period becomes longer.in sweden, the geographical distribution of lyme borreliosis corresponds to that of its vector ixodes ricinus. both tick activity and the length of the vegetation period are determined by daily mean temperatures ≥5°c. we analysed the correspondence between the distribution of i. ricinus in sweden, the start date, end date, and length of the vegetation period, and the distributions of tick habitat-associated plant species. the geographical distribution of i. ricinus in sweden corresponds to a veg ...201121771536
the past, present and future tasks of hungarian dendrological research.hungarian dendrological research (research of living woody plants) has more than 200 years old history; the first general work by j+ínos kereszt+®ly grossinger was published in 1797. further basic works in our time yet are: forest botany by lajos fekete and s+índor m+íg+¦csy-dietz (1896); and the chorological work, distribution of trees and shrubs of sylvicultural importance in the region of hungarian state by lajos fekete and tibor blattny (1913). a few dendrologists and many botanists have hel ...201021565760
metallohistins: a new class of plant metal-binding proteins.two small multimeric histidine-rich proteins, agnt84 and ag164, encoded by two nodule-specific cdnas isolated from nodule cdna libraries of the actinorhizal host plant alnus glutinosa, represent a new class of plant metal binding proteins. this paper reports the characterization of the purified in vitro-expressed proteins by size exclusion chromatography, circular dichroism, equilibrium dialysis, metal affinity chromatography coupled with mass spectrometry, and nuclear magnetic resonance spectro ...200212638655
Liberomyces gen. nov. with two new species of endophytic coelomycetes from broad-leaved trees.During a study of endophytic and saprotrophic fungi in the sapwood and phloem of broad-leaved trees (Salix alba, Quercus robur, Ulmus laevis, Alnus glutinosa, Betula pendula), fungi belonging to an anamorphic coelomycetous genus not attributable to a described taxon were detected and isolated in pure culture. The new genus Liberomyces with two species, L. saliciphilus and L. macrosporus, is described. Both species have subglobose conidiomata containing holoblastic sympodial conidiogenous cells. ...201121937729
[maples at the sub-alpine vegetation belt: a long history].pollen analysis was carried out on lacustrine sediment of a small hollow (15 m x 25 m) at the treeless sub-alpine belt (202 m) of the inner maurienne valley in the northern french alps. a 2,500-year-long maple settlement was demonstrared. three ams dates of terrestrial plant macroremains support the chronology. first, betula and salix spread prior to 9,000 c14 bp. the first pollen grains of acer, abies and pinus cembra are quoted at 8,600 c14 bp. high frequencies of alnus glutinosa/incana (20%) ...200111280048
characterization of a casuarina glauca nodule-specific subtilisin-like protease gene, a homolog of alnus glutinosa ag12.in search of plant genes expressed during early interactions between casuarina glauca and frankia, we have isolated and characterized a c. glauca gene that has strong homology to subtilisin-like protease gene families of several plants including the actinorhizal nodulin gene ag12 of another actinorhizal plant, alnus glutinosa. based on the expression pattern of cg12 in the course of nodule development, it represents an early actinorhizal nodulin gene. our results suggest that subtilisin-like pro ...200010656592
pet-prof, a plasmid for high-level expression of recombinant peptides fused to a birch profilin-derived hexadecapeptide tag: a system for the detection and presentation of recombinant antigens.we have previously identified a birch pollen profilin hexadecapeptide (bp36/51), which was recognized by a monoclonal antibody (moab 4a6) with high affinity. here, we report the construction of a t7 rna polymerase-driven high-level plasmid expression system, pet-prof, capable of producing proteins and peptides containing the bp36/51 birch profilin-derived peptide fused to their n-terminus. as examples, the cdnas coding for two major timothy grass (phleum pratense) pollen allergens, phl p 2 and p ...199910521657
bacterial-induced calcium oscillations are common to nitrogen-fixing associations of nodulating legumes and nonlegumes.plants that form root-nodule symbioses are within a monophyletic 'nitrogen-fixing' clade and associated signalling processes are shared with the arbuscular mycorrhizal symbiosis. central to symbiotic signalling are nuclear-associated oscillations in calcium ions (ca(2+) ), occurring in the root hairs of several legume species in response to the rhizobial nod factor signal. in this study we expanded the species analysed for activation of ca(2+) oscillations, including nonleguminous species within ...201526010117
[the hepatoprotective action of ellagotannins].the hepatoprotective properties of altan (obtained on the basis of ellagotannines from the cones of black alder alnus glutinosa) are studied on the model of acute damage to the liver with tetrachloromethane. it is found that altan exhibits the hepatoprotective activity even in a dose of 1 mg/kg which is tenfold smaller compared to the dose of traditional flavonoid-based drugs. altan limits choleopoiesis disorder, has an antiinflammatory and membrane stabilizing effect, and recovers physiological ...19989854636
phylogenetic characterization of ineffective frankia in alnus glutinosa (l.) gaertn. nodules from wetland soil inoculants.ineffective frankia endophytes were retrieved from various wet soils by using alnus glutinosa clones as trapping plants. no pure cultures could be isolated from these ineffective nodules. therefore, the phylogenetic position of these endophytes was determined by sequence analysis of cloned pcr products of bacterial 16s rdna, derived from nodules. the results showed that all nodule endophytes belong to a hitherto undescribed cluster of the frankia phylogenetic tree. the position of these uncultur ...19979348704
identification of agthi1, whose product is involved in biosynthesis of the thiamine precursor thiazole, in actinorhizal nodules of alnus glutinosa.a cdna clone, pagthi1, encoding a homologue of yeast thi4, which is involved in thiazole biosynthesis, was isolated from a library made from poly(a) rna from actinorhizal nodules of alnus glutinosa by differential screening with nodule and root cdna, respectively. the corresponding gene, agthi1, was shown to be expressed at high levels in nodules and shoot tips of a. glutinosa, while having low expression levels in roots, flowers, and developing fruits. the function of agthi1 was demonstrated by ...19968771789
a nodule-specific gene encoding a subtilisin-like protease is expressed in early stages of actinorhizal nodule development.to identify genes specifically expressed during early stages of actinorhizal nodule development, a cdna library made from poly(a) rna from root nodules of alnus glutinosa was screened differentially with nodule and root cdna, respectively. seven nodule-enhanced and four nodule-specific cdna clones were isolated. by using in situ hybridization, two of the nodule-specific cdnas were shown to be expressed at the highest levels in infected cells before the onset of nitrogen fixation; one of them, ag ...19957647567
identification of frankia strains in nodules by hybridization of polymerase chain reaction products with strain-specific oligonucleotide probes.a set of oligonucleotides has been developed to study the competitivity of two frankia strains in the nodulation of the roots of two host plant species: alnus glutinosa and alnus incana. two 20 mer-oligonucleotides, complementary to highly conserved sequences inside the nifh gene, were used as primers for the polymerase chain reaction (pcr) system in order to amplify microsymbiont dna extracted from actinorhizae. pcr products were analyzed using two strain-specific 15-mer oligonucleotides identi ...19902334247
homology of the major birch-pollen allergen, bet v i, with the major pollen allergens of alder, hazel, and hornbeam at the nucleic acid level as determined by cross-hybridization.to investigate the relationship of the major allergens of birch (bet v i), alder (aln g i), hazel (cor a i), and hornbeam (car b i) at the nucleic acid level, a cdna clone coding for the complete bet v i protein was used for northern and southern blot experiments. rnas were isolated from pollen of birch (betula verrucosa), alder (alnus glutinosa), hazel (corylus avellana), and hornbeam (carpinus betulus). hybridization was performed at different stringencies. at high stringency, comparable bindi ...19912005320
complementary dna cloning and expression in escherichia coli of aln g i, the major allergen in pollen of alder (alnus glutinosa).previous data showed that the major pollen allergens from trees of the order fagales, in particular alder, birch, hazel, and hornbeam, are highly interrelated. as only the complete amino acid sequence of bet v i, the major allergen from birch, has been known, it was of interest to obtain the primary structure of other major allergens of this group, to attribute ige-binding properties to certain features of the amino acid sequences of those allergens. cdna was synthesized from alder pollen mrna, ...19921460197
in vitro dermo-cosmetic evaluation of bark extracts from common temperate trees.wood residues produced from forestry activities represent an interesting source of biologically active, high value-added secondary metabolites. in this study, 30 extracts from 10 barks of deciduous and coniferous tree species were investigated for their potential dermo-cosmetic use. the extracts were obtained from fagus sylvatica, quercus robur, alnus glutinosa, prunus avium, acer pseudoplatanus, fraxinus excelsior, populus robusta, larix decidua, picea abies, and populus tremula after three suc ...201627352384
allergen-induced in vitro expression of il-18, slam and gata-3 mrna in pbmc during sublingual immunotherapy.signalling lymphocytic activation molecule (slam) and interleukin (il)-18 induce interferon (ifn)-gamma production from th1 cells. the allergen-induced slam and il-18 mrna expressions are increased during subcutaneous immunotherapy (scit), but nothing is known about their role during sublingual immunotherapy (slit). transcription factor gata-3 is associated with th2 cells but its role in scit and slit is yet unexplored. this study was undertaken to analyse the allergen induced in vitro mrna expr ...200717620074
molecular and immunologic characterization of a highly cross-reactive two ef-hand calcium-binding alder pollen allergen, aln g 4: structural basis for calcium-modulated ige recognition.serum ige was used to isolate a cdna coding for a 9.4-kda two ef-hand calcium-binding allergen, aln g 4, from a lambda gt11 expression cdna library constructed from alder (alnus glutinosa) pollen. raln g 4 was overexpressed in escherichia coli and purified to homogeneity. it reacted with serum ige from 18% of pollen-allergic patients (n = 122); shared ige epitopes with homologous allergens present in tree, grass, and weed pollens; and thus belongs to a family of highly cross-reactive pollen alle ...19989862740
evidence that some frankia sp. strains are able to cross boundaries between alnus and elaeagnus host specificity groups.phenotypic and genotypic methods were used to prove the existence of frankia strains isolated from an elaeagnus sp. that are able to cross the inoculation barriers and infect alnus spp. also. repeated cycles of inoculation, nodulation, and reisolation were performed under axenic conditions. frankia wild-type strain ufi 13270257 and three of its coisolates did exhibit complete infectivity and effectiveness on elaeagnus spp. and hippophaë rhamnoides and variable infectivity on alnus spp. microscop ...19921352442
effect of tree species and mycorrhizal colonization on the archaeal population of boreal forest rhizospheres.group 1.1c crenarchaeota are the predominating archaeal group in acidic boreal forest soils. in this study, we show that the detection frequency of 1.1c crenarchaeotal 16s rrna genes in the rhizospheres of the boreal forest trees increased following colonization by the ectomycorrhizal fungus paxillus involutus. this effect was very clear in the fine roots of pinus sylvestris, picea abies, and betula pendula, the most common forest trees in finland. the nonmycorrhizal fine roots had a clearly dif ...200918978075
photosynthetic and leaf water potential responses of alnus glutinosa saplings to stem-base inoculaton with phytophthora alni subsp. alni.three-year-old alnus glutinosa (l.) gaertn. (alder) saplings were single or double inoculated at the stem base with phytophthora alni subsp. alni brasier & s.a. kirk under natural climatic conditions. lesion formation on the bark showed a biphasic pattern of development, with extension occurring at a moderate rate in spring, and more rapidly during late summer. however, large variability was encountered in pathogen development within the population of infected saplings, ranging from high suscept ...200818765375
survey of microfungi on alnus glutinosa (l.) gaertn. from münsterland, germany.during our investigation on microfungi in antoniusheim, fleissenbach und merfelderbruch near dülmen in münsterland in the years 2005 and 2006 we were able to collect and identify 25 microfungi on alnus glutinosa (l.) gaertn. among them are some which are very rare in germany linke phragmoporthe conformis (berkley & broome) petrak, cryptosporiopsis alnea (rostr.) petr., prosthecium auctum (berk. & broome) petr., taphrina alni-incanae (kun.) magn. [= t. amentorum (sadeback) rostrup], cryptodiaport ...200718396820
tree species composition affects the abundance of rowan (sorbus aucuparia l.) in urban forests in finland.recent studies have shown a considerable increase in the abundance of rowan (sorbus aucuparia) saplings in urban forests in finland, yet the reasons for this increase are not well understood. here we investigated whether canopy cover or tree species composition, i.e., the basal areas of different tree species in norway spruce dominated urban forests, affects the abundances of rowan seedlings, saplings and trees. altogether 24 urban forest patches were investigated. we sampled the number of rowan ...201525588119
saprophytic growth of inoculated frankia sp. in soil microcosms.the potential of two frankia strains to grow saprophytically was studied in nonsterile soil microcosms with ground leaf litter of alnus glutinosa as the sole carbon and nitrogen sources. strains ag45/mut15 and ari3, which represent two taxonomic subgroups within the alnus host infection group were inoculated alone, or together to investigate potential competition. their growth was analyzed by in situ and dot-blot hybridization. a significant increase in cell numbers and filament length was obser ...200717916077
revisiting ectomycorrhizal fungi of the genus alnus: differential host specificity, diversity and determinants of the fungal community.actinorhizal plants, including those of the genus alnus (alders; betulaceae), and their nitrogen-fixing bacterial symbionts rely on mycorrhizal fungi for phosphorus and other mineral nutrients. to date, alders are known to associate with only 20-30 species of ectomycorrhizal fungi which are highly host-specific. this study aimed to determine the species richness and the relative importance of host species, soil and site variables on the community composition of alnus-associated ectomycorrhizal f ...200919320837
both the arbuscular mycorrhizal fungus gigaspora rosea and frankia increase root system branching and reduce root hair frequency in alnus glutinosa.alnus glutinosa is an important pioneer species that forms effective symbioses with frankia and ecto and arbuscular mycorrhizal fungi (amf). there is evidence that frankia and amf interact and the focus of this study was to investigate how interactions affected root system and root hair development. a. glutinosa seedlings were grown in pots in soil pre-inoculated with the amf gigaspora rosea. seedlings were inoculated with frankia either immediately on transfer to amf-inoculated pots (day 0) on ...201019690898
control of endophytic frankia sporulation by alnus nodule metabolites.a unique case of microbial symbiont capable of dormancy within its living host cells has been reported in actinorhizal symbioses. some frankia strains, named sp+, are able to sporulate inside plant cells, contrarily to sp- strains. the presence of metabolically slowed-down bacterial structures in host cells alters our understanding of symbiosis based on reciprocal benefits between both partners, and its impact on the symbiotic processes remains unknown. the present work reports a metabolomic stu ...201728072559
nature of fly ash amendments differently influences oxidative stress alleviation in four forest tree species and metal trace element phytostabilization in aged contaminated soil: a long-term field experiment.aided phytostabilization using coal fly ashes (cfas) is an interesting technique to clean-up polluted soils and valorizing industrial wastes. in this context, our work aims to study the effect of two cfas: silico-aluminous (cfa1) and sulfo-calcic (cfa2) ones, 10 years after their addition, on the phytostabilization of a highly cd (cadmium), pb (lead) and zn (zinc) contaminated agricultural soil, with four forest tree species: robinia pseudoacacia, alnus glutinosa, acer pseudoplatanus and salix a ...201728061412
foliar methyl salicylate emissions indicate prolonged aphid infestation on silver birch and black alder.it is well documented that when plants are damaged by insects they respond by emitting a range of volatile organic compounds (vocs). while there have been numerous reports concerning vocs induced by chewing herbivores, there are relatively few studies detailing the vocs induced by aphid feeding. the effects of aphid feeding on vocs emitted by boreal forest trees have been particularly neglected. herbivore-induced vocs have relevance to direct and indirect plant defence and atmospheric chemistry. ...201020097686
the frankia alni symbiotic transcriptome.the actinobacteria frankia spp. are able to induce the formation of nodules on the roots of a large spectrum of actinorhizal plants, where they convert dinitrogen to ammonia in exchange for plant photosynthates. in the present study, transcriptional analyses were performed on nitrogen-replete free-living frankia alni cells and on alnus glutinosa nodule bacteria, using whole-genome microarrays. distribution of nodule-induced genes on the genome was found to be mostly over regions with high synten ...201020367468
flooding induced emissions of volatile signalling compounds in three tree species with differing waterlogging tolerance.to gain insight into variations in waterlogging responsiveness, net assimilation rate, stomatal conductance, emissions of isoprene and marker compounds of anoxic metabolism ethanol and acetaldehyde, and stress marker compounds nitric oxide (no), volatile products of lipoxygenase (lox) pathway and methanol were studied in seedlings of temperate deciduous tree species alnus glutinosa, populus tremula and quercus rubra (from highest to lowest waterlogging tolerance) throughout sustained root zone w ...201020444211
flash-flood impacts cause changes in wood anatomy of alnus glutinosa, fraxinus angustifolia and quercus pyrenaica.flash floods may influence the development of trees growing on channel bars and floodplains. in this study, we analyze and quantify anatomical reactions to wounding in diffuse-porous (alnus glutinosa l.) and ring-porous (fraxinus angustifolia vahl. and quercus pyrenaica willd.) trees in a mediterranean environment. a total of 54 cross-sections and wedges were collected from trees that had been injured by past flash floods. from each of the samples, micro-sections were prepared at a tangential di ...201020462937
diversity of frankia strains nodulating hippophae salicifolia d. don using fame profiling as chemotaxonomic markers.twelve frankia strains isolated from hippophae salicifolia d. don or alnus glutinosa or comptonia peregrine, showed the significant variation in fatty acid composition viz. palmitic acid (16:0), oleic acid (18:1), linoleic acid (18:2), linolenic acid (18:3), arachidic acid (20:0) and erucic acid (22:1) suggesting the strain specific variability among the frankia strains. presence of erucic acid (22:1), a major component of the oil obtained from the seeds of brassica sp., albeit in lesser amount ...201020473962
frankia populations in soil and root nodules of sympatrically grown alnus taxa.the genetic diversity of frankia populations in soil and in root nodules of sympatrically grown alnus taxa was evaluated by rep-polymerase chain reaction (pcr) and nifh gene sequence analyses. rep-pcr analyses of uncultured frankia populations in root nodules of 12 alnus taxa (n=10 nodules each) growing sympatrically in the morton arboretum near chicago revealed identical patterns for nodules from each alnus taxon, including replicate trees of the same host taxon, and low diversity overall with ...201120838787
biotic and abiotic variables influencing plant litter breakdown in streams: a global study.plant litter breakdown is a key ecological process in terrestrial and freshwater ecosystems. streams and rivers, in particular, contribute substantially to global carbon fluxes. however, there is little information available on the relative roles of different drivers of plant litter breakdown in fresh waters, particularly at large scales. we present a global-scale study of litter breakdown in streams to compare the roles of biotic, climatic and other environmental factors on breakdown rates. we ...201627122551
development of bacterial community during spontaneous succession on spoil heaps after brown coal mining.changes in the abundance of bacteria and fungi and in the composition of bacterial communities during primary succession were investigated in a brown coal mine deposit area near sokolov, the czech republic, using phospholipid fatty acids analysis, microarray and 16s rrna gene sequencing. the study considered a chronosequence of sites undergoing spontaneous succession: 6-, 12-, 21- and 45-year-old and a 21-year-old site revegetated with alnus glutinosa. during succession, organic carbon and the t ...201121707674
N(2) fixation and cycling in Alnus glutinosa, Betula pendula and Fagus sylvatica woodland exposed to free air CO(2) enrichment.We measured the effect of elevated atmospheric CO(2) on atmospheric nitrogen (N(2)) fixation in the tree species Alnus glutinosa growing in monoculture or in mixture with the non-N(2)-fixing tree species Betula pendula and Fagus sylvatica. We addressed the hypotheses that (1) N(2) fixation in A. glutinosa will increase in response to increased atmospheric CO(2) concentrations, when growing in monoculture, (2) the impact of elevated CO(2) on N(2) fixation in A. glutinosa is the same in mixture an ...201122179329
tree age effects on fine root biomass and morphology over chronosequences of fagus sylvatica, quercus robur and alnus glutinosa stands.there are few data on fine root biomass and morphology change in relation to stand age. based on chronosequences for beech (9-140 years old), oak (11-140 years) and alder (4-76 years old) we aimed to examine how stand age affects fine root biomass and morphology. soil cores from depths of 0-15 cm and 16-30 cm were used for the study. in contrast to previously published studies that suggested that maximum fine root biomass is reached at the canopy closure stage of stand development, we found almo ...201626859755
in-planta sporulation capacity enhances infectivity and rhizospheric competitiveness of frankia strains.frankia sp+ strains maintain their ability to sporulate in symbiosis with actinorhizal plants, producing abundant sporangia inside host plant cells, in contrast to sp- strains, which are unable to perform in-planta sporulation. we herein examined the role of in-planta sporulation in frankia infectivity and competitiveness for root infection. fifteen strains belonging to different sp+ and sp- phylogenetic lineages were inoculated on seedlings of alnus glutinosa (ag) and a. incana (ai). strain com ...201626726131
tree species diversity interacts with elevated co2 to induce a greater root system response.as a consequence of land-use change and the burning of fossil fuels, atmospheric concentrations of co2 are increasing and altering the dynamics of the carbon cycle in forest ecosystems. in a number of studies using single tree species, fine root biomass has been shown to be strongly increased by elevated co2 . however, natural forests are often intimate mixtures of a number of co-occurring species. to investigate the interaction between tree mixture and elevated co2 , alnus glutinosa, betula pen ...201223504733
the combined effects of water level reduction and an increase in ammonia concentration on organic matter processing by key freshwater shredders in alluvial wetlands.in a global change context, the intensity and the frequency of drastic low flow periods or drought events will most likely increase to a substantial extent over the coming decades, leading to a modification in the abiotic characteristics of wetlands. this change in environmental parameters may induce severe shifts in plant and animal communities and the functioning of ecosystems. in this study, we experimentally estimated the effect of drought and the accumulation of ammonia (nh3 ) on the feedin ...201223504834
identification of potential transcriptional regulators of actinorhizal symbioses in casuarina glauca and alnus glutinosa.trees belonging to the casuarinaceae and betulaceae families play an important ecological role and are useful tools in forestry for degraded land rehabilitation and reforestation. these functions are linked to their capacity to establish symbiotic relationships with a nitrogen-fixing soil bacterium of the genus frankia. however, the molecular mechanisms controlling the establishment of these symbioses are poorly understood. the aim of this work was to identify potential transcription factors inv ...201425492470
genetic transformation of 9 in vitro clones of alnus and betula by agrobacterium tumefaciens.crown gall tumorigenesis, integration and expression of t-dna encoded genes from agrobacterium tumefaciens were investigated in 9 clones of alnus glutinosa, a. incana and betula papyrifera. tumor formation on in vitro shoots was frequent in all clones with strain ach5 and present in 8 clones with strain c58. tumors excised from shoots were selected for autotrophic growth in vitro and axenic cultures were established. octopine or nopaline, respective of the strain type used for inoculation, was d ...198824241754
antimicrobial activity of some alnus species.the increasing prevalence of resistant microorganisms forced scientists to find new antimicrobial substances from different sources like medicinal plants. the aim of this study was to determine the antimicrobial activities of leaf extracts of some alnus sp. against some bacteria and a yeast.201526698267
total phenolic and flavonoid contents, antioxidant and antimicrobial activities of alnus glutinosa (l.) gaertn., alnus incana (l.) moench and alnus viridis (chaix) dc. extracts.the objective of this study was to determine total phenolic and flavonoid contents, antioxidant and antimicrobial activities of methanolic extracts from the leaves and barks of three alnus species. the phenolic and flavonoid contents of extracts were determined spectrophotometrically using folin-ciocalteau and aluminium chloride methods, respectively. in addition, antioxidant activity of the extracts was determined using 1,1-diphenyl-2-picrylhydrazyl radical scavenging method. the antimicrobial ...201424969264
diarylheptanoids from alnus viridis ssp. viridis and alnus glutinosa: modulation of quorum sensing activity in pseudomonas aeruginosa.diarylheptanoids from the barks of alnus viridis ssp. viridis (green alder) and alnus glutinosa (black alder) were explored for anti-quorum sensing activity. chemicals with anti-quorum sensing activity have recently been examined for antimicrobial applications. the anti-quorum sensing activity of the selected diarylheptanoids was determined using two biosensors, namely pseudomonas aeruginosa pao1 and chromobacterium violaceum cv026. although all of the investigated compounds negatively influence ...201727220074
tech-ia floating system introduced in urban wastewater treatment plants in the veneto region - italy.the performance of three integrated wetland treatment plants (horizontal sub-surface flow (h-ssf) and floating treatment wetland (ftw) with differentiated primary treatments) designed for treating domestic wastewater was investigated, monitoring total (tn), nitrate (no3-n), nitrite (no2-n) and ammonia nitrogen (nh4-n), total (tp) and phosphate phosphorus (po4-p), chemical (cod) and biological oxygen demand (bod5), and dissolved oxygen (do) at the inlet and outlet of each wetland section from feb ...201324037167
bioactivity-guided identification of antimicrobial metabolites in alnus glutinosa bark and optimization of oregonin purification by centrifugal partition chromatography.barks from conifers and broadleaved trees constitute abundant wastes generated from wood harvesting and logging activities. extracts of such residues obtained from alnus trees have been reported as interesting resources with potent antibacterial activities. the present study aims to determine the antimicrobial activity of a crude methanol extract prepared from the bark of alnus glutinosa against a panel of 22 bacteria and yeasts and to optimize a purification method enabling the high production ...201627428455
ecotoxicity effects triggered in aquatic organisms by invasive acer negundo and native alnus glutinosa leaf leachates obtained in the process of aerobic decomposition.the replacement of autochthonous tree species by invasive ones in coastal zones of freshwater bodies induces additional alteration of hydrochemical and microbiological characteristics due to decomposition of fallen leaves of non-indigenous species, which can lead to ecotoxic response of the littoral biota. leaves of invasive to lithuania boxelder maple (acer negundo) and autochthonous black alder (alnus glutinosa) lost more than half of biomass and released stable amount of doc (60-70 mg/l) thro ...201425058932
leaf mineral nutrient remobilization during leaf senescence and modulation by nutrient deficiency.higher plants have to cope with fluctuating mineral resource availability. however, strategies such as stimulation of root growth, increased transporter activities, and nutrient storage and remobilization have been mostly studied for only a few macronutrients. leaves of cultivated crops (zea mays, brassica napus, pisum sativum, triticum aestivum, hordeum vulgare) and tree species (quercus robur, populus nigra, alnus glutinosa) grown under field conditions were harvested regularly during their li ...201526029223
ecotoxicological effects evoked in hydrophytes by leachates of invasive acer negundo and autochthonous alnus glutinosa fallen off leaves during their microbial decomposition.throughout 90-day biodegradation under microaerobic conditions, invasive to lithuania species boxelder maple (acer negundo) leaves lost 1.5-fold more biomass than that of autochthonous black alder (alnus glutinosa), releasing higher contents of n(tot), ammonium and generating higher bod(7). boxelder maple leaf leachates were characterized by higher total bacterial numbers and colony numbers of heterotrophic and cellulose-decomposing bacteria than those of black alder. the higher toxicity of a. n ...201323202636
selective behaviour of honeybees in acquiring european propolis plant precursors.honey bees harvest resins from various plant species and use them in the hive as propolis. while there have been a number of studies concerning the chemical composition of this antimicrobial product, little is known about selective behavior and bee preference when different potential plant sources of resin are available. the main objective of this paper was to investigate some aspects of behavioral patterns of honeybees in the context of resin acquisition. samples of propolis originating from te ...201627294416
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