| studies on lectins. xxxvi. properties of some lectins prepared by affinity chromatography on o-glycosyl polyacrylamide gels. | a number of lectins has been purified by affinity chromatography on o-glycosyl polyacrylamide gels. the lectins isolated (and the particular sugar ligands used in the affinity carriers) are as follows: anguilla anguilla, serum (alpha-l-fucosyl-), vicia cracca, seeds; phaseolus lunatus, seeds; glycine soja, seeds; dolichos biflorus, seeds; maclura pomifera, seeds; sarothamnus scoparius, seeds; helix pomatia, ablumin glands; clitocybe nebularis, fruiting bodies (all n-acetyl-alpha-d-galactosaminyl ... | 1978 | 563738 |
| changes in glycoconjugates revealed by lectin staining in the developing airways of syrian golden hamsters. | lectin binding was studied in the developing airways of syrian golden hamsters on gestational days 11-16 (day 16 is the day of birth). the trachea and lungs were fixed in 4% formaldehyde-1% glutaraldehyde, 6% mercuric chloride-1% sodium acetate-0.1% glutaraldehyde, and 95% ethanol; embedded in paraffin; and stained with eight lectin-horseradish peroxidase conjugates: triticum vulgare (wga), dolichos biflorus (dba), helix pomatia (hpa), maclura pomifera (mpa), griffonia simplicifolia i-b4 (gsa i- ... | 1990 | 1700650 |
| cell type-specific lectin staining of the tracheobronchial epithelium of the rat: quantitative studies with griffonia simplicifolia i isolectin b4. | we compared lectin staining patterns to cell population densities, as determined by morphological criteria in rat airways. eight lectins were studied: griffonia simplicifolia i isolectin b4 (gsi-b4), arachis hypogaea (pna), wisteria floribunda (wfa), glycine maximus (sba), dolichos biflorus (dba), helix pomatia (hpa), ulex europaeus (uea-1), and maclura pomifera (mpa). two of the lectins strongly stained morphologically distinct cell subpopulations. gsi-b4 stained basal cells, and mpa stained no ... | 1991 | 1701188 |
| surfactant lipid uptake and secretion in type ii cells in response to lectins and secretagogues. | secretion of surfactant phospholipids can be inhibited by the surfactant-associated protein sp-a. sp-a was reported to stimulate lipid uptake by type ii cells, whereas surfactant secretagogues were reported to have the same effect in isolated perfused lungs. we examined the effect of such secretagogues on uptake of liposomes containing l-alpha-[2-palmitoyl-9,103h(n)]-dipalmitoyl phosphatidylcholine in primary cultures of type ii cells. as sp-a contains a lectinlike domain and other lectins were ... | 1991 | 1767864 |
| lectin histochemistry of developing submandibular glands of fetal syrian golden hamsters. | lectin binding was studied in the developing submandibular glands of fetal syrian golden hamsters (mesocricetus auratus) from gestational day 12 to 16 (the day of birth). the fetuses were fixed, embedded in paraffin, sectioned and stained with nine lectin-horseradish peroxidase conjugates: concanavalin a (con a), wheat germ agglutinin (wga), dolichos biflorus agglutinin (dba), helix pomatia agglutinin (hpa), maclura pomifera agglutinin (mpa). griffonia simplicifolia agglutinin i-b4 (gsa i-b4), p ... | 1991 | 2035849 |
| characterization of galactose-containing glycoconjugates in the human retina: a lectin histochemical study. | seven specimens of morphologically normal formalin-fixed and paraffin-embedded human retina were studied using a panel of fourteen biotinylated lectins, all of which react with glycoconjugates containing galactose and n-acetylgalactosamine residues. agaricus bisporus (aba), bauhinia purpurea (bpa), phaseolus vulgaris (pha-e), peanut (pna) and ricinus communis (rca-i) agglutinins labeled photoreceptor cells prior to enzymatic predigestion. bpa and pna bound specifically to cones. the plexiform la ... | 1990 | 2091899 |
| glycoconjugate histochemistry in the glycocalyx of guinea pig middle ear mucosa. | an attempt was made to determine the composition of oligosaccharide side chains in the apical cell surface glycocalices of the middle ear epithelium. this glycoconjugate is located on the innermost luminal surface, but its function is still unknown. the lectins evaluated included triticum vulgare, canavalia ensiformis, maclura pomifera, arachis hypogaea, ricinus communis i (rca-i), and limax flavus. the staining results obtained with the six different lectin probes in the lowicryl k4m-embedded g ... | 1990 | 2393562 |
| identification of sporozoite surface proteins and antigens of eimeria nieschulzi (apicomplexa). | sodium dodecyl sulfate polyacrylamide gel electrophoresis, immunoblotting, lectin binding, and 125i surface labeling of sporozoites were used to probe sporozoites of the rat coccidian, eimeria nieschulzi. analysis of silver stained gels revealed greater than 50 bands. surface iodination revealed about 14 well labeled, and about 10 weakly labeled but potential, surface proteins. the most heavily labeled surface proteins had molecular masses of 60, 53-54, 45, 28, 23-24, 17, 15, 14, 13, and 12 kd. ... | 1990 | 2319491 |
| effects of lectins with various carbohydrate binding specificities on lipid metabolism in isolated rat and hamster adipocytes. | 1. mannose-binding and n-acetylglucosamine binding lectins exhibited potent antilipolytic and lipogenic activities. 2. fucose-binding lectins had minimal lipogenic activity but possessed antilipolytic activity. 3. most galactose-binding and n-acetylgalactosamine-binding lectins were devoid of significant antilipolytic and lipogenic activities. notable exceptions were lectins from momordica charantia, wisteria floribunda, vicia villosa, codium fragile and the siberian pine tree. lipogenic activit ... | 1989 | 2545472 |
| ontogeny of pulmonary alveolar epithelial markers of differentiation. | we studied differentiation of the pulmonary epithelium in the periphery of fetal rat lung in vivo and in vitro by comparing the ontogeny of cell-surface glycoconjugates with that of surfactant phospholipids. apical surface binding of the lectin maclura pomifera agglutinin (mpa) and expression of a 200-kda mpa-binding glycoprotein (mpa-gp200) was evident at 20 days gestation in type 2 cells, but did not correlate with ultrastructural features of type 2 cell differentiation. epithelial cells isola ... | 1990 | 2303166 |
| lectin binding beneath the epithelium and in smooth muscle cells in the developing bronchial tree. | components of the subepithelial stratum in developing rat lung reacted transiently with maclura pomifera agglutinin (mpa) and aleuria aurantia agglutinin (ofa) conjugated to horseradish peroxidase. these lectins possess selective affinity for and serve to localize glycoconjugates (gcs) with terminal gal/galnac and fuc, respectively. staining was strongest with both lectins in the proximal bronchial tree and decreased peripherally to growing buds where it was absent. mpa staining of subepithelial ... | 1990 | 2224573 |
| expression of cell-specific markers for alveolar epithelium in fetal rat lung. | in adults, the alveolar epithelium is composed of types i and ii cells which differ structurally and functionally although they appear to belong to the same cell lineage. using cell-specific markers (type i cells, monoclonal antibody; type ii cells, maclura pomifera lectin [mpa]), we have determined when and in what pattern their binding sites occur during development of the fetal rat lung. rather than first appearing on days 19 to 20, when morphogenesis of type i cells occurs and lamellar bodie ... | 1990 | 2189457 |
| comparison of glycoconjugates at the surface of developing type ii pneumocytes and clara cells. | the apical surface coat of type ii pneumocytes and clara cells in pre- and post-natal rat lung was examined with lectin histochemical methods. lectins from helix pomatia (hpa), peanut (pna) and maclura pomifera (mpa) were conjugated with horseradish peroxidase and used to stain paraffin sections of fixed lung with or without certain pre-treatments. hpa and mpa were observed to react with almost all type ii pneumocytes at postnatal day 1. type ii pneumocytes that stained with a sialidase-pna sequ ... | 1989 | 2777637 |
| contact lens wear enhances adherence of pseudomonas aeruginosa and binding of lectins to the cornea. | extended wear soft contact lenses are associated with an increased incidence of pseudomonas aeruginosa keratitis. because the first step in the pathogenesis of this disease is adherence of the microorganism to the corneal surface, we studied the effect of soft contact lens wear on the adherence of p. aeruginosa to the cornea. rabbits were fitted for extended wear soft contact lenses in the left eye, and the right eye served as a control. both eyes were then closed with a partial tarsorrhaphy. af ... | 1990 | 2115422 |
| isolation and partial characterization of pneumocin, a novel apical membrane surface glycoprotein marker of rat type ii cells. | rat alveolar type ii pneumocytes, in situ, label with maclura pomifera agglutinin (mpa), a plant lectin that recognizes alpha-galactosyl oligosaccharide residues of glycoproteins and glycolipids. to study the glycoproteins recognized by the lectin, mpa lectin affinity chromatography was used to isolate a novel glycoprotein, pneumocin, from type ii and whole rat lung cell membranes. pneumocin isolated from adult rat lungs was a non-disulfide-linked sialoglycoprotein with an mr of 165 kd. asparagi ... | 1991 | 2054190 |
| uptake and subcellular distribution of escherichia coli lipopolysaccharide by isolated rat type ii pneumocytes. | treatment of isolated rat type ii pneumocytes with escherichia coli lipopolysaccharide (lps) induces a number of ultra-structural changes which become evident after 60 min of incubation. by using post-embedding immunolabeling methods and electron microscopy, we have followed the fate of lps after different times of incubation. after an initial period of accumulation in the pneumocyte microvilli, the lps molecules enter the cytoplasm, forming discrete patches which are dispersed in some areas. af ... | 1991 | 2016511 |
| comparison of the maclura pomifera lectin-binding glycoprotein in late fetal and adult rat lung. | the lectin, maclura pomifera agglutinin (mpa), binds to alpha-galactose residues of glycoproteins on the apical surface of type ii alveolar cells. it has recently been shown to bind to macrophages. we isolated the cell surface glycoprotein, which binds the mpa lectin, from fetal and adult rat whole lung to determine if changes in this glycoprotein occur during development from fetal to adult life. the glycoprotein was purified from whole lung cell membranes by lectin affinity chromatography that ... | 1991 | 1895777 |
| morphological study of lectin binding in the rabbit temporomandibular joint disc. | glycoconjugates of the extracellular matrix are important for the normal mechanical functions of connective tissue structures such as the temporomandibular joint disc. since lectins are known to bind to sugar residues with high affinity, a variety of lectins were used to study the presence and distribution of glycoconjugates in the temporomandibular joint disc. discs were removed from 6 to 8-month-old rabbits and either sectioned in a cryostat and processed for light microscopy or fixed in 2% gl ... | 1991 | 1783561 |
| differential lectin binding on walls of thoraco-cervical blood vessels and lymphatics in rats. | lectin binding in the walls of large to medium-sized blood vessels and lymphatics in the rat thoraco-cervical region was examined histochemically. the tunica intima of the aorta and superficial cervical artery showed positive reactions with wheat germ agglutinin (wga) and concanavalin a (cona) but not with dolichus biflorus agglutinin (dba). the tunica media of the aorta exhibited intense wga binding, especially on the smooth muscle cells, but the tunica media of the superficial cervical artery ... | 1991 | 1758681 |
| heterogeneity of apical glycoconjugates in kidney collecting ducts: further studies using simultaneous detection of lectin binding sites and immunocytochemical detection of key transport enzymes. | in the search for a functional role for the polarized glycoconjugates of rat collecting duct epithelial cells, the relation between binding of various lectins and expression of cellular transport enzyme profile of the cells was studied. for this purpose, principal and intercalated cells of rat kidney collecting duct were identified by morphological criteria and by their immunocytochemically determined content of (na+ + k+)-atpase and carbonic anhydrase (ca ii), respectively. various n-acetylgala ... | 1988 | 2853698 |
| a rat alveolar type ii cell line developed by adenovirus 12se1a gene transfer. | the regulation of pulmonary alveolar type ii cell proliferation and differentiation is poorly understood and has been difficult to study, in part due to lack of proliferation, cellular heterogeneity, and phenotypic instability of type ii cells in primary culture. to develop a stable population of homogeneous cells capable of proliferation, we transfected type ii cells isolated from the lungs of neonatal rats with an immortalizing oncogene, adenovirus 12se1a, using a retroviral vector. individual ... | 1992 | 1728294 |
| differential expression of galactose and n-acetylgalactosamine residues during fetal development and postnatal maturation of rat glomeruli as revealed with lectin conjugates. | a battery of fluorochrome- or peroxidase-coupled lectins, reacting with alpha- or beta-galactose (gal), terminal n-acetylgalactosamine (galnac), or gal-(beta 1-3)-galnac residues, was used to study the emergence and distribution of cellular glycoconjugates in developing and adult rat glomeruli. neuraminidase pretreatment of the specimens was applied to monitor the maturation of the glomerular sialoglycoprotein coat. in the adult glomeruli, the lectin conjugates applied reacted sparsely or not at ... | 1989 | 2923282 |
| subcompartment sugar residues of gastric surface mucous cells studied with labeled lectins. | we examined the intracellular localization of sugar residues of the rat gastric surface mucous cells in relation to the functional polarity of the cell organellae using preembedding method with several lectins. in the surface mucous cells, the nuclear envelope and rough endoplasmic reticulum (rer) and cis cisternae of the golgi stacks were intensely stained with maclura pomifera (mpa), which is specific to alpha-gal and galnac residues. in the golgi apparatus, one or two cis side cisternae were ... | 1991 | 1708750 |
| lectin histochemistry applied to human nerves. | histologic sections of normal and pathologically altered human peripheral nerves were stained with a battery of 20 fluorescein isothiocyanate-labeled lectins to determine whether these histochemical reagents could be used to identify peripheral nerve injury. eight plant lectins--from canavalia ensiformis, lens culinaris, triticum vulgare (wheat germ), types e4 and l4 from phaseolus vulgaris, types i and ii from ricinus communis, and wistaria floribunda--were found to bind to normal and pathologi ... | 1986 | 3015069 |
| identification of specific glomerular cell types in culture by use of lectin and antibody binding. | to characterize the outgrowth of cells from cultured rat glomeruli, we examined the cell morphology in relation to the expression of various antigenic determinants (factor viii-related antigen, rat thy 1.1, heyman nephritogenic antigen, common leucocyte and ia antigens) and lectin binding patterns. for this purpose, early (3-7 days) to late (4-5 weeks) glomerulus cultures, as well as subcultures of mesangial cells were used. in the early samples, the predominance of small round (type i) cells gr ... | 1990 | 1698520 |
| serum accelerates the loss of type ii cell differentiation in vitro. | the differentiated phenotype of the alveolar type ii cell is rapidly altered in vitro. to evaluate factors that might influence this process, we isolated and plated rat type ii cells in serum-supplemented media to promote adherence and then maintained the cells in a simple nutrient medium in the absence (s- cells) or presence (s+ cells) of serum for 5 to 7 d. the type ii s- cells remained metabolically active. despite protein synthesis that was 50% that of s+ cells, s- cells continued to synthes ... | 1990 | 1698400 |
| reproducible isolation of type ii pneumocytes from fetal and adult rat lung using nycodenz density gradients. | isolating fresh, relatively pure type ii pneumocytes from the lung, particularly of fetal origin, is a difficult process. separation by buoyant density gradient centrifugation has been used successfully to isolate adult type ii cells. there is concern, however, that percoll, a gradient medium that is commonly used for type ii cell isolation, may be toxic to cells. we evaluated a new gradient medium, nycodenz, that is (1) a true solution, (2) transparent, (3) not metabolized by cells, and (4) non ... | 1992 | 1572331 |
| glycoconjugates of the human sperm surface: distribution and alterations that accompany capacitation in vitro. | we have studied changes in the binding of fluoresceinated lectins to human sperm during in vitro capacitation. we first determined the surface labeling pattern of viable sperm obtained by the swim-up procedure. sperm were labeled with 100 micrograms/ml fitc-conjugated lectin at 4 degrees c for 30 min. we simultaneously used hoechst stain 33258 as a supravital stain to help differentiate surface from intracellular lectin labeling. of 14 lectins studied, six (phytohemagglutinin-e, concanavalin a, ... | 1987 | 3147937 |
| flow cytometric analyses of lectin binding to pneumocystis carinii surface carbohydrates. | pneumocystis carinii obtained from infected rat lung homogenates was incubated with fluorescein isothiocyanate-conjugated lectins, counterstained with the nuclear stain, propidium iodide (pi), and analyzed by dual parameter histograms for lectin-associated green and pi-associated red fluorescence using a fluorescence-activated cell sorter. the presence of glucose/mannose moieties was evidenced by the binding of all organisms to concanavalin a and wisteria floribunda. from the lectin group specif ... | 1992 | 1556643 |
| human sperm surface mapping with lectins. | ten fluorescein isothiocyanate (fitc)-linked lectins [bauhimia purpurea, concanavalin a, dolichos biflorus (dba), griffonia simplicifolia i, griffonia simplicifolia ii, maclura pomifera, arachis hypogea (pna), glycine max, ulex europaeus (uea) and triticum vulgaris agglutinin] have been used to study their binding features on the human ejaculate spermatozoa. qualitative changes in the labeling pattern have been observed in unfixed and acetone-treated spermatozoa. furthermore, ultrastructural loc ... | 1992 | 1466231 |
| distribution of glycoconjugates localized by peanut and maclura pomifera agglutinins during mouse molar root development. | glycolipids, glycoproteins, glycosaminoglycans and sialoglycoproteins have all been implicated in a number of developmentally significant processes related to complex interactions between cell surfaces and the extracellular matrix. the present study was designed to localize glycoconjugates recognized by peanut agglutinin (pna) and maclura pomifera (mpa) lectins during mouse molar root development. postnatal icr mice at 10, 15, 21, 28 and 42 days were used. lower jaws were dissected, fixed in 4% ... | 1992 | 1441887 |
| cellular heterogeneity in human transitional cell carcinoma: an analysis of optical properties and lectin binding. | flow cytometry was used to measure the binding of a panel of ten fluorescein isothiocyanate(fitc)-conjugated lectins to fifteen samples of normal and neoplastic human urothelium. concurrent measurement of light scattering and fluorescence permitted the quantification of lectin binding to cellular subpopulations defined by their light-scattering properties. in normal urothelium, we previously demonstrated levels of lectin binding to the cellular subpopulations derived from the superficial and int ... | 1992 | 1429004 |
| an antibody with specificity for surfactant protein c precursors: identification of pro-sp-c in rat lung. | surfactant protein c (sp-c) is a lung-specific, hydrophobic peptide found in organic extracts of pulmonary surfactant. alveolar sp-c (3.5 kd) is produced from proteolytic cleavage of a larger precursor molecule (pro-sp-c; 21 kd). while sp-c is synthesized by type ii cells, the pathways for processing and secretion have remained elusive due, in part, to the lack of monospecific antibodies against sp-c or its precursors. this report describes production and characterization of a new antibody direc ... | 1992 | 1389209 |
| isolation and immortalization of rat pre-type ii cell lines. | the fetal respiratory distress syndrome is due, in part, to the presence of abundant pre-type ii alveolar epithelial cells that have not yet differentiated into mature type ii cells. studies of this syndrome have been limited somewhat by the lack of an adequate in vitro model. in the present study we immortalized pre-type ii cells by infecting primary isolates obtained from fetal rat lung with a retroviral construct expressing the adenoviral 12s e1a gene product. the immortalized pre-type ii cel ... | 1992 | 1316350 |
| a carbohydrate-deficient membrane glycoprotein in human erythrocytes of phenotype s-s-. | 1. we investigated the membranes of human erythrocytes which completely lack the blood-group antigens s and s (denoted as s-s-) as part of a study of the structure and function of the surface glycoproteins of the human erythrocyte. 2. the s-s-erythrocyte-membrane glycoprotein pas-3 band was much less intensely stained in comparison with that of the glycoprotein from normal erythrocyte membranes. the s-s-membrane glycoprotein pas-4 band also showed decreased staining. 3. examination with the lect ... | 1977 | 889570 |
| purification and properties of the hemagglutinin from maclura pomifera seeds. | maclura promifera seeds contain a protein which agglutinates human erythrocytes at concentrations as low as 4 ng/ml. this property is related to its ability to bind with high specificity various alpha-d-galactopyranosides. the agglutinin, which was pruified by affinity adsorption, exhibits one band on immunoelectrophoresis and displays one peak during ultracentrifugation, isoelectric focusing, and gel permeation chromatography. the active protein has a molecular weight of 40 000-43 000 and conta ... | 1977 | 588553 |
| binding of islet cell cytoplasmic antibody from insulin-dependent diabetes mellitus patients and plant lectin (maclura pomifera agglutinin) to pancreas islet cells. | | 1986 | 3541858 |
| cytotoxicity of lymphoid cells induced by maclura pomifera (mp) lectin. | | 1979 | 311694 |
| glycophorin is the reovirus receptor on human erythrocytes. | purified glycophorin (predominantly type a) from human erythrocytes was found to effectively inhibit reovirus hemagglutination (ha) in contrast to other glycoproteins such as fetuin or ovalbumin. glycophorin was also a potent inhibitor of reovirus and protein sigma 1 binding to mouse l fibroblasts. glycophorin pretreated with neuraminidase lost these inhibitory properties. using a solid phase binding assay, it was demonstrated that reovirus as well as protein sigma 1 could specifically bind to g ... | 1987 | 3604060 |
| analysis of n-linked oligosaccharide chains of glycoproteins on nitrocellulose sheets using lectin-peroxidase reagents. | a rapid and convenient method was established for analysis of the n-linked carbohydrate chains of glycoproteins on nitrocellulose sheets. proteins were separated by polyacrylamide gel electrophoresis, transferred to nitrocellulose sheets, reacted with peroxidase-coupled lectins, and detected by color development of the enzyme reaction. four glycoproteins having n-linked oligosaccharide chains were used as test materials: taka-amylase a (which has a high-mannose-type chain), ovalbumin (high-manno ... | 1985 | 2411164 |
| [membrane changes in duchenne/becker muscular dystrophy: lectin binding and localization of dystrophin]. | an rca i-lectin binding glycoprotein of mr = 370 kd is missing from or altered in the plasma membrane of duchenne muscular dystrophy (dmd) skeletal muscle. in the present study the carbohydrate chain of this glycoprotein was localized to the external face of the plasma membrane in human skeletal muscle, and dystrophin, the protein product of the dmd gene, was localized to the inner (cytoplasmic) face. on double labelled western blots the two proteins appeared as closely apposed but distinctly se ... | 1989 | 2646527 |
| homology of the d-galactose-specific lectins from artocarpus integrifolia and maclura pomifera and the role of an unusual small polypeptide subunit. | the maclura pomifera agglutinin (mpa) was purified by affinity chromatography from a seed extract and its properties were compared with those of the artocarpus integrifolia lectin, jacalin. reverse-phase high-performance liquid chromatography showed both proteins had multiple forms of a small approximately 20-residue polypeptide chain in addition to the major 12,000 mr subunit. the amino acid sequences of the small chains and the n-terminal sequences of the large subunits showed considerable sim ... | 1989 | 2705782 |
| glycosylation of developing human glomeruli: lectin binding sites during cell induction and maturation. | expression of cellular glycoconjugates during differentiation of human fetal kidney was studied using fluorochrome-labeled lectins. each lectin revealed a characteristic binding pattern during the phenotypic change of the nephrogenic mesenchyme and during distinct stages of nephron development. the uninduced mesenchymal cells were positive for pisum sativum (psa), concanavalin a (cona), wistaria floribunda (wga), and ricinus communis (rca-i) lectins. however, these lectins failed to react with t ... | 1987 | 3794308 |
| alveolar cell differentiation markers in human lungs. | we describe two apical surface integral membrane glycoproteins which appear to be differentiation markers of the human pulmonary alveolar type 2 cell which has as a major function the production of pulmonary surfactant. these membrane glycoproteins bind the lectin, maclura pomifera agglutinin and can be found in detergent extract of whole lungs, lung membranes and isolated type 2 cells. one of the mpa binding glycoproteins (mpa-gp330) has an apparent molecular weight of 330 kd and is analogous t ... | 1989 | 2733026 |
| a histochemical localization on maclura pomifera lectin during osteogenesis. | mandibular condyles of 4-week-old wistar strain rats and mandibles of icr strain mice from 14 days gestation stage to 2 days postnatal stage were used to investigate the localization of maclura pomifera lectin (mpa) during two modes of osteogenesis. during endochondral ossification of the mandibular condyle, mpa was only localized at the peripheral regions of calcified cartilage after the destruction of chondrocyte lacunae. bone extracellular matrix (ecm) was not reacted with mpa. in intramembra ... | 1989 | 2777640 |
| lectins as probes to pneumocystis carinii surface glycocomplexes. | the binding characteristics of a panel of commercially available fitc-conjugated lectins to pneumocystis carinii (pc) were assessed by fluorescence microscopy and flow cytometry. rat pc obtained from infected lung homogenates were incubated with fitc-conjugated lectins in a series of concentrations, counterstained with propidium iodide, and analyzed for percent fluorescence and fluorescence intensity. all organisms bound concanavalin a and wisteria floribunda agglutinin, 2 representatives of the ... | 1989 | 2785201 |
| studies of lectin binding to the human cervix uteri: i. normal cervix. | lectins of bauhinia purpurea (bpa), canavalin ensiformis (con a), griffonia simplicifolia i (gs i), griffonia simplicifolia ii (gs ii), maclura pomifera (mpa), arachis hypogaea (pna), glycine max (sba), ulex europaeus i (uea i) and triticum vulgaris (wga) were used to evaluate cell surface carbohydrates in formalin-fixed paraffin-embedded tissue sections of normal human cervix uteri. consistent patterns of staining of the squamous epithelium were obtained in all 30 cases with bpa, gs ii, mpa, pn ... | 1989 | 2793521 |
| studies of lectin binding to the human cervix uteri: ii. cervical intraepithelial neoplasia and invasive squamous carcinoma. | the cell surface carbohydrate profile of formalin-fixed paraffin-embedded tissue sections of neoplastic cervical squamous epithelium was evaluated using lectins of bauhinia purpurea (bpa), canavalin ensiformis (con a), griffonia simplicifolia i (gs i), griffonia simplicifolia ii (gs ii), maclura pomifera (mpa), archis hypogaea (pna), glycine max (sba), ulex europaeus i (uea i) and triticum vulgaris (wga). three lectins (bpa, con a and pna) showed a similar pattern of staining in both normal squa ... | 1989 | 2793522 |
| the distribution and characterization of the erythrocyte. receptors in man and animals for lectins from maclura pomifera. | | 1967 | 4167911 |
| isolation and characterization of maclura pomifera (mp) lectin. | | 1974 | 4417994 |
| lectin histochemistry in rat thyroid tumours. | the thyroid tumours and background goiterous and adenomatous lesions induced in rats with diisopropanolnitrosamine (dipn) plus methylthiouracil (mtu), and regenerative thyroid tissues after wounding were studied by lectin histochemistry. ten weeks after cessation of the carcinogen treatments, carcinomas invading the surrounding tissues and blood vessels (13/20) and papillary micronodules (11/20) were formed in the thyroid tissues. in general, the carcinoma lesion was solitary, and the papillary ... | 1989 | 2818422 |
| cell type-specific glycoconjugates of collecting duct cells during maturation of the rat kidney. | the ontogeny of lectin-positive epithelial cell types and the maturation of polarized expression of the glycocalyx of the collecting ducts (cd) of the rat kidney were studied from samples of 18th-day fetal and neonatal kidneys of various ages. lectins from dolichos biflorus (dba) and vicia villosa (vva), with preferential affinity to principal cells, stained virtually all cd cells of the fetal kidneys. however, within two days postnatally, the number of cells positive for dba and vva decreased t ... | 1988 | 2842054 |
| identification of a 185 kd maclura pomifera agglutinin binding glycoprotein as a candidate for a differentiation marker for alveolar type ii cells in adult rat lung. | in adult rat lung the lectin maclura pomifera agglutinin (mpa) binds apically to alveolar type ii (atii) cells but not to alveolar type i (ati) cells. this suggests that the presence of mpa binding glycoproteins might be a criterion by which to distinguish the differentiated state of these two adult alveolar epithelial cells. the authors therefore studied mpa binding glycoproteins of atii cells, comparing, biochemically and cytochemically, mpa binding glycoproteins in freshly isolated atii cells ... | 1989 | 2916648 |
| isolation of pulmonary alveolar type i cells from adult rats. | the authors have developed a procedure for the isolation of alveolar type i (ati) cells from adult rat lung. after an initial selective enzymatic digestion of the lungs by lavage with 0.2% collagenase, 0.05% trypsin, 0.008% elastase, and 0.005% dnase type i, the cells which are released are separated by density gradient centrifugation, and a fraction which includes all ati cells (density, 1.0177-1.0411) is harvested. contaminating leukocytes are excluded by specific surface adsorption, exploitin ... | 1986 | 2945439 |
| detection of surface carbohydrates on pneumocystis carinii by fluorescein-conjugated lectins. | lectins react with a wide range of different carbohydrates (table 1). even so-called monospecific anti-h(o) lectins from lotus tetragonolobus, ulex europaeus, and anguilla anguilla react not only with the anti-h determinant but also with several fucosylated carbohydrates. consequently, the type of lectin receptor existing on the surface of pneumocystis carinii should be determined, because only a carbohydrate analysis can demonstrate the kind of carbohydrates which exist on the cell surface of t ... | 1987 | 3125541 |
| pneumocystis carinii: surface reactive carbohydrates detected by lectin probes. | pneumocystis carinii obtained from rat lungs (rlh) and in vitro culture (rtc) were reacted with a panel of 14 fluorescein isothiocyanate conjugated lectins. percentage fluorescence and fluorescent intensity were determined for both trophic and cyst forms. all rlh and rtc derived organisms bound strongly concanavalin a (con a), and wheat germ agglutinin (wga). however, differences in soybean agglutinin (sba) binding between rlh and rtc organisms was observed. different subsets of the organism bou ... | 1988 | 3142787 |
| immunochemical studies on pneumococcal type 37 capsular polysaccharide. | pneumococcal type 37 capsular polysaccharide was obtained free of contaminants by affinity chromatography on con-a, wheat germ agglutinin, maclura pomifera lectin and hopc-8 mouse myeloma protein affinity columns. the immunochemical reactivity of native and periodate oxidized borohydride reduced type 37 polysaccharide antigen with polyclonal rabbit and monoclonal mouse anti-pn37 hybridoma antibodies was studied by quantitative precipitation. quantitative hapten inhibition studies, employing the ... | 1988 | 3146022 |
| human pulmonary alveolar type 2 cells contain an apical membrane glycoprotein common to malignant cells. | a monoclonal antibody, ca1, raised against a detergent extract of hep 2 cells derived from a human laryngeal carcinoma, was shown in these studies to bind to the apical surface of normal alveolar type 2 cells but not type 1 cells in the human lung. in lung specimens from patients with alveolitis, the antibody also bound to hyperplastic type 2 cells and to transition cells which were in the process of becoming alveolar type 1 cells. ca1 binds to the apical plasma membrane and to internal membrane ... | 1988 | 3172711 |
| glomerular sialoconjugates of developing and mature rat kidneys. | the appearance of sialoconjugates in developing rat kidney glomeruli was studied using lectins and neuraminidase-lectin staining sequences. in the early s-shaped bodies, binding of maclura pomifera (mpa; specific for galactosaminyl residues of glycoconjugates) could be detected in the presumptive podocyte layer at the apex of these cells, but notably no binding of lectins specific for sialic acid could be seen. during further morphologic maturation of the s-shaped bodies, binding of limax flavus ... | 1988 | 3208291 |
| regulation of surfactant phospholipid secretion from isolated rat alveolar type ii cells by lectins. | the major surfactant-associated protein is a potent inhibitor of surfactant phospholipid secretion from isolated type ii cells. since the major surfactant-associated protein contains a carboxy terminal polypeptide domain which is homologous to the lectin-like liver mannose-binding protein, we tested whether lectins inhibit surfactant phospholipid secretion from rat alveolar type ii cells. concanavalin a, wheat germ agglutinin and maclura pomifera agglutinin were potent inhibitors of surfactant p ... | 1988 | 3337835 |
| histochemical and immunocytochemical identification of alveolar type ii epithelial cells isolated from fetal rat lung. | primary cultures of epithelial cells isolated from organotypic cultures of fetal (days 18 through 22) rat lung have been characterized by histochemical and immunocytochemical parameters. immunocytologic analysis with monoclonal antibodies to cytokeratins and with those to adult type ii cells (jbr-1) demonstrated that the cell cultures were composed almost entirely of epithelial type ii cells. additional evidence that the cultures had the type ii phenotype was obtained by maclura pomifera lectin ... | 1988 | 3345034 |
| identification and characterization of the pulmonary alveolar type ii cell maclura pomifera agglutinin-binding membrane glycoprotein. | the lectin maclura pomifera agglutinin (mpa) binds to the apical surface of pulmonary alveolar type ii but not type i cells. we show that mpa binds to a single membrane glycoprotein in type ii cells with a molecular mass of 230 kda in the rabbit and 200 kda in the rat. the glycoprotein has an abundance of terminal n-acetylgalactosamine residues. it is a hydrophilic integral membrane protein suggesting that it has an extensive extramembrane domain or is an ion channel. the glycoprotein is similar ... | 1988 | 3416017 |
| [selective damage of pulmonary nonciliated bronchiolar epithelial (clara) cells by trichloroethylene in rats]. | ethanol-treated and ethanol-nontreated rats were exposed to 0, 500, 1,000, 2,000, 4,000 and 8,000 ppm of trichloroethylene for 2 h in order to evaluate the metabolic (metabolic rate of styrene) and morphologic changes in the lung. the exposure resulted in dose-dependent decrease of styrene metabolism. by mpa (maclura pomifera agglutinin) stain it was revealed by light microscopy that trichloroethylene caused a highly selective damage to nonciliated bronchiolar epithelial (clara) cells, which wer ... | 1988 | 3418956 |
| lectin binding sites on human endocervix: a comparison with secretory and proliferative endometrium. | endocervix and corresponding endometrium of women of reproductive age were studied histochemically with 13 fluorescein isothiocyanate-labeled lectins to delineate the differences between the epithelial cells in two anatomical sites. lectin from maclura pomifera (mpa), ulex europaeus (uea-i), glycine max (sba), and vicia villosa (vva) bound only to endocervical epithelium and were the only four lectins that distinguished endocervical from endometrial epithelium. these differences were independent ... | 1986 | 3526979 |
| isolation and characterization of differentiated alveolar type ii cells from fetal human lung. | a method has been developed for isolating differentiated type ii cells from human lung of 18-24-week gestation. the procedure involves an initial 4-day culture of lung explants in the presence of dexamethasone (10 nm) and triiodothyronine (2 nm). type ii cells (and fibroblasts) are isolated by trypsin digestion of the explants, two differential adherence steps and incubation overnight in primary culture. this method provides a high yield of type ii cells ((50 +/- 15) x 10(6) cells/g wet weight o ... | 1986 | 3527277 |
| griffonia simplicifolia lectins bind specifically to endothelial cells and some epithelial cells in mouse tissues. | the binding of griffonia simplicifolia agglutinin-i (gsa-i) and the isolectins gsa-i-ab3 and gsa-i-b4, having affinity for some alpha-d-galactosyl and n-acetyl galactosaminyl residues was studied in different mouse tissues. in brain, cardiac muscle and skeletal muscle, the gsa-i-lectin conjugates showed prominent binding only to blood vessel endothelia. similarly, in the liver and kidney cortex the gsa-i-conjugates selectively reacted with endothelial cells of the sinusoids and with intertubular ... | 1987 | 3597137 |
| culture of differentiated and undifferentiated type ii cells from fetal rat lung. | we have developed a relatively simple and reproducible method for the isolation and culture of both differentiated and undifferentiated type ii cells from fetal rat lung. the technique involves an initial period of explant culture in serum and hormone free medium, followed by enzymatic dissociation of the explants, differential adhesion to remove fibroblasts, incubation of the cell pellet to promote aggregation of the type ii cells and monolayer culture of the type ii cells. the type ii cells fo ... | 1987 | 3620509 |
| cellular heterogeneity in normal human urothelium: an analysis of optical properties and lectin binding. | flow cytometry was used to study the optical properties of normal urothelial cells in suspension. narrow-angle light scatter, which is a function of cell size, defined one major and one minor cell population, and 90 degrees light scatter, a function of intracellular structure, showed three distinct cell populations. these properties were displayed as a 2-dimensional dot plot or "fingerprint" which proved to be characteristic and reproducible from one specimen of urothelium to the next. cell sort ... | 1986 | 3754881 |
| a comparison of lectin binding in rat and human peripheral nerve. | eleven different fluorescein- or peroxidase-conjugated lectins with different sugar-binding affinities were employed to analyze and compare glycoconjugates of rat and human peripheral nerves at the light microscopic level. a majority of lectins showed a distinct binding pattern in different structures of the nerve. lectin binding was similar but not identical in rat and human nerves. limulus polyhemus agglutinin did not stain any structures in rat or human nerves. in both species, all other lect ... | 1986 | 3772078 |
| interaction of salmonella telaviv with maclura pomifera lectin. | salmonella telaviv, salmonella tranoroa, and salmonella illinois were examined for their ability to interact with 15 purified lectins of known sugar specificity. the only interaction observed was between the lectin of maclura pomifera and s. telaviv. m. pomifera lectin specifically agglutinated suspensions of s. telaviv and precipitated with its purified lipopolysaccharide and isolated lipid a free o polysaccharide. quantitative inhibition assays showing methyl-alpha-d-galactopyranoside and n-ac ... | 1985 | 3838092 |
| changes in biochemical characteristics and pattern of lectin binding of alveolar type ii cells with time in culture. | when cultured on plastic culture dishes for several days, alveolar type ii cells gradually lose both their morphologic and biochemical identifying characteristics. although type ii cells cultured on a matrix derived from corneal endothelial cells have previously been reported to retain lamellar bodies for 7-10 days in culture, the ability of type ii cells cultured on matrix to synthesize surfactant lipids has not been previously studied. we therefore measured the phospholipid content and the dis ... | 1985 | 3839418 |
| the large sialoglycoprotein of human lymphocytes. ii. biochemical features. | large sialoglycoprotein of human lymphocytes (l-lsgp) from thymocytes and from peripheral blood lymphocytes (pbl) of normal donor and of b chronic lymphocytic leukemia (cll) patients was purified by affinity chromatography to maclura pomifera agglutinin (mpa)-sepharose followed by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). l-lsgp from the three different sources was very similar in amino acid composition. it contained a high proportion of acidic and hydroxy ... | 1985 | 3873337 |
| an immunofluorescent analysis of lectin binding to normal and regenerating skeletal muscle of rat. | ten different fluorescein-conjugated lectins of various known sugar specificities were used to study cell surface glycoconjugates of normal and regenerating rat skeletal muscle. in normal muscle, canavalia ensiformis agglutinin, triticum vulgaris agglutinin (wheat germ agglutinin, wga), ricinus communis agglutinin-i, and maclura pomifera agglutinin bound strongly to the endomysial region of the myofibers. no binding was observed in the cytoplasm of the myofibers. other lectins (dolichos biflorus ... | 1985 | 3915936 |
| a sperm-agglutinating lectin from seeds of jack fruit (artocarpus heterophyllus). | a lectin specific for n-acetylgalactosamine was isolated from seed extract of jack fruit (artocarpus heterophyllus) by ammonium sulfate precipitation, followed by affinity chromatography on a affigel-galactosamine-agarose column. the lectin possessed agglutinating activities for human and rat sperm as well as human red blood cells. it was found to have mr = 62,000 consisting of two dissimilar subunits of mr = 18,000 and 13,000. it also cross-reacted with an antibody against the lectin of osage o ... | 1985 | 3922368 |
| binding of maclura pomifera lectin to rat lymphoid cells and erythrocytes. | | 1973 | 4584403 |
| the binding of maclura pomifera lectin to cells of the t-lymphocyte lineage in the rat. | | 1982 | 6122507 |
| uptake of lectins by pulmonary alveolar type ii cells: subsequent deposition into lamellar bodies. | type ii cells of the alveolar epithelium of adult rats have been shown to internalize the ferritin-labeled lectin from maclura pomifera (mpa-f). this alpha-galactose-binding molecule binds specifically to the apical plasma membrane of the cells. once within the cell the lectin is cycled from pinocytic vesicles, to multivesicular bodies of two types, and finally to lamellar bodies, the storage granules of surfactant. those multivesicular bodies that first contain mpa-f lack detectable lysosomal e ... | 1984 | 6149544 |
| interaction of lectins with neisseria gonorrhoeae. | fifty-four strains of neisseria gonorrhoeae were examined for their ability to be agglutinated by various lectins. wheat germ agglutinin, ricin, soybean lectin, and peanut agglutinin agglutinated all strains tested. dolichos biflorus, sophora japonica, maclura pomifera, ulex europaeus, lens culinaris, canavalia ensiformis, phaseolus lunatus, and bandeiraea simplicifolia bs i and bs ii lectins either failed to agglutinate or agglutinated some strains but not others. agglutination of gonococci by ... | 1980 | 6155190 |
| rabbit anti-carbohydrate antibody elicited by the lymphocyte mitogenic glycoprotein from wistaria floribunda seeds. | rabbit antibody produced in response to the purified mitogenic glycoprotein lectin from wistaria floribunda seeds (wfm) contains anti-carbohydrate antibody. this antibody, which represents 25% of the total antibody precipitated by the homologous antigen cross-reacts with the glycoprotein hemagglutinating lectins from sophora japonica (sjl), w. floribunda (wfa) and the glycoprotein bromelain, but not the protein lectin from maclura pomifera seeds. the cross-reactive reaction is totally abolished ... | 1983 | 6412071 |
| pregnancy-related changes in the mouse oviduct and uterus revealed by differential binding of fluoresceinated lectins. | the binding of 20 fluorescein isothiocyanate (fitc)-labeled lectins to various portions of the pregnant and non-pregnant murine oviduct and uterus was studied by fluorescence microscopy. five lectins (from ricinus communis (rca-i), maclura pomifera (mpa), triticum vulgare (wheat germ-wga), bauhinia purpurea (bpa), and ulex europeus (uea-i] reacted differentially with the epithelium of pregnant as compared with the non-pregnant uterus. the binding of rca-i, mpa and wga delineated pregnancy-relate ... | 1983 | 6418694 |
| lectin binding sites of the mouse ovary, intraovarian and ovulated ova. | fluorescein isothiocyanate (fitc) labeled lectins were used to study the distribution of specific binding sites in histologic sections of mouse ovaries as well as ovulated ova. four distinct patterns of reactivity of the components of the follicle (exclusive of the ovum) and the surrounding ovarian stroma were recognized: uniform staining of granulosa cells, theca cells and surrounding stroma; weak to moderate staining of the granulosa cells and strong staining of the theca cells and stromal cel ... | 1984 | 6432745 |
| peripheral nervous system myelin and schwann cell glycoproteins: identification by lectin binding and partial purification of a peripheral nervous system myelin-specific 170,000 molecular weight glycoprotein. | radioiodinated lectins were used to detect glycoproteins of peripheral nervous system (pns) myelin (rat, human, bovine) and cultured rat schwann cells. proteins were resolved by sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis and transferred to nitrocellulose filters. the filters were overlaid with radioiodinated lectins of known saccharide affinities. these included concanavalin a, helix pomatia, limulus polyphemus, maclura pomifera, peanut, soybean, ulex europaeus, and wheat ger ... | 1983 | 6619865 |
| lectins as markers of human epidermal cell differentiation. | the expression of sugar residues on human epidermal cells was investigated by means of lectin binding, as a way of determining membrane structural changes occurring during the differentiation of the epidermis. fourteen lectins of different sugar specificity were conjugated with fluorescein isothiocyanate (fitc-lectins) and tested in fluorescence microscopy on frozen sections of normal human epidermis. in parallel, fitc-lectins were tested on psoriatic-involved epidermis to visualize differences ... | 1982 | 6816653 |
| isolation of the maclura pomifera hemagglutinin on a deoxymelibiotol affinity support and preliminary characterization by buffer electrofocusing and high-performance liquid chromatography. | maclurin, the potent non-specific blood-group hemagglutinin present in extracts of maclura pomifera, has been purified by a new biospecific affinity-chromatographic procedure. additional studies have indicated that this hemagglutinin occurs as five closely related tetrameric protein isoforms derived from two non-covalently-linked polypeptide chains, mol. wts. ca. 10,000 and 13,000 respectively. buffer electrofocusing fractionated the lectin into 12 components; the major isolectin exhibited an is ... | 1982 | 6896160 |
| five structurally related proteins from affinity-purified maclura pomifera lectin. | affinity-purified maclura pomifera lectin (mpl) elutes from a gel filtration column as a single symmetrical peak with characteristics expected for a single protein of approximately 40 000 daltons. this material can be dissociated into two dissimilar polypeptide chains of approximately 10 000 daltons. ion-exchange chromatography on deae-cellulose resolves affinity-purified mpl into five components. these proteins are structurally related and contain varying proportions of the two polypeptide chai ... | 1981 | 7236627 |
| a new human erythrocyte variant (ph) containing an abnormal membrane sialoglycoprotein. | 1. a new human erythrocyte variant (ph) is described. the variant contains an unusual sialic acid-rich glycoprotein in addition to the blood-group-mn([unk])- and blood-group-ss(delta)-active sialoglycoproteins found in normal erythrocytes. 2. the unusual component ph has an apparent mol.wt. of 32000 on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. the ph component is not degraded during trypsin treatment of intact erythrocytes. 3. the ph component was labelled by lacto-peroxidase-m ... | 1980 | 7396858 |
| interaction of native and asialo rat sublingual glycoproteins with lectins. | the binding properties of the rat sublingual glycoprotein (rsl) and its asialo product with lectins were characterized by quantitative precipitin(qpa) and precipitin inhibition(qpia) assays. among twenty lectins tested for qpa, native rsl reacted well only with artocarpus integrifolia (jacalin), but weakly or not at all with the other lectins. however, its asialo product (asialo-rsl) reacted strongly with many gal and galnac specific lectins-it bound best to three of the galnac alpha 1-->ser/thr ... | 1995 | 7475931 |
| localization of surfactant protein a (sp-a) in alveolar macrophage subpopulations of normal and fibrotic rat lung. | the colocalization of surfactant protein a (sp-a) and the alveolar macrophage markers ed1 and rm-1, as well as various lectins of the n-acetyl-galactosamine group [maclura pomifera lectin (mpa), dolichos biflorus lectin (dba), soybean agglutinin (sba)] and of the mannose group [canavalia ensiformis lectin (cona), galanthus nivalis lectin (gna)] was studied in normal and fibrotic rat lung tissues. in normal tissue, sp-a was located preferentially in the alveolar macrophage subpopulation lacking s ... | 1994 | 7532637 |
| purification of murine pulmonary type ii cells for flow cytometric cell cycle analysis. | mice are widely used as animal models for in vivo lung disease. despite this fact, few methods exist for isolation of type ii pneumocytes from mouse lung, limiting the study of alveolar epithelial characteristics in these models. this study investigated several methods for labeling murine lung cell suspensions for flow cytometric identification and sorting of type ii pneumocytes. crude lung cell suspensions were prepared after intratracheal instillation of dispase and were labeled using phosphin ... | 1995 | 7621777 |
| patterns of lectin binding during mammalian neurogenesis. | temporospatial changes in surface carbohydrates of neuroepithelial cells were analysed by means of lectin histochemistry in normal mouse embryos subsequent to closure of the neural tube. the lectins used were concanavalin a (con a), soybean (sba), maclura pomifera (mpa), peanut (pna), wheatgerm (wga), succinylated wheatgerm (swga) and limax flavus (lfa). although labelling was obtained with all of the lectins, the most striking temporospatial differences occurred with con a which in the early em ... | 1995 | 7649814 |
| heterogeneity in the immunolocalization of cytokeratin-specific monoclonal antibodies in the rat lung: evaluation of three different alveolar epithelial cell types. | the distribution of individual cytokeratin polypeptides in the adult rat lung parenchyma was investigated by immunohistochemistry with 44 monoclonal and 2 polyclonal antibodies. simple epithelial cytokeratins 7, 8, 18 and 19 were found to be expressed differently in alveolar and bronchial epithelial cells. three distinct types of alveolar cells were detected according to their pattern of immunoreactivity: type ii cells strongly expressing cytokeratins 8 and 18 and weakly expressing cytokeratins ... | 1993 | 7693628 |
| native and asialo-tamm-horsfall glycoproteins as important ligands for the detection of galnac beta 1-->and gal beta 1-->4glcnac active lectins. | the binding properties of human tamm-horsfall sd(a+) urinary glycoprotein(thgp) and asialo-thgp with various applied lectins was investigated by quantitative precipitin and precipitin inhibition assays. both glycoproteins completely precipitated abrus precatorius agglutinin(apa). they also reacted well with wistaria floribunda (wfa), glycine max (soybean, sba), and ricinus communis agglutinins and precipitated over 78% of the lectin nitrogen added, but reacted poorly or weakly with all alpha-ano ... | 1995 | 7726822 |
| changes in glycan distribution within the porcine interhaemal barrier during gestation. | changing patterns of glycan distribution are described in porcine placentae at 15, 19, 26, 43, 58, 69 and 109 days gestation, using a carefully selected panel of lectins that allowed partial analysis of saccharide classes and sequences. the lectins used were from galanthus nivalis, pisum sativum, phaseolus vulgaris (leukohaemagglutinin), triticum vulgaris, tetragonolobus purpureus, ulex europaeus-1, arachis hypogaea, erythrina cristagalli, glycine max, maclura pomifera, wisteria floribunda, doli ... | 1995 | 7736552 |
| a sheep hydatid cyst glycoprotein as receptors for three toxic lectins, as well as abrus precatorius and ricinus communis agglutinins. | the binding properties of a glycoprotein with blood group p1 specificity isolated from sheep hydatid cyst fluid with gal and galnac specific lectins was investigated by quantitative precipitin and precipitin inhibition assays. the glycoprotein completely precipitated ricinus communis agglutinin (rca1), abrus precatorius agglutinin (apa) and mistletoe toxic lectin-i (ml-i). only 1.0 microgram of p1 glycoprotein was required to precipitate 50% of 5.1 micrograms ml-i nitrogen. it also reacted well ... | 1995 | 7827100 |
| lectin-histochemical analysis of glycans in ovine and bovine near-term placental binucleate cells. | chorionic binucleate cells (bnc) occur in several ruminants including cow, deer, goat and sheep. they migrate through the chorionic tight junction to fuse with uterine epithelial cells and discharge their granules into maternal connective tissue. we have compared the bnc of near-term, resin-embedded, ovine and bovine placentae using 15 biotinylated lectins and an avidinperoxidase revealing system. there was pronounced conservation of saccharides between the two species. several sub-types of n-gl ... | 1994 | 7850870 |
| bauhinia purpurea lectin (bpa) binding of rat type i pneumocytes: alveolar epithelial alterations after radiation-induced lung injury. | in the rat lung, we found that the bauhinia purpurea lectin (bpa) specifically binds to the type i alveolar epithelial cells and to the alveolar macrophages. double label fluorescence employing fitc-coupled maclura pomifera lectin (mpa) and bbpa-avidin-texas red showed that bpa binding was confined to type i cells. in addition, a minor staining of the luminal border of the terminal bronchiolar epithelium was found. the sequence of tissue injury following x-radiation was examined in rats. bpa is ... | 1994 | 7894248 |
| confocal imaging of time-dependent internalization and localization of nbd-pc in intact rat lungs. | morphological localization of a fluorescent analogue of phosphatidylcholine (pc), c12-nbd-pc ([1-palmitoyl-2-[12-(7-nitro-2-1,3-benzoxadiazol-4-yl) amino] dodecanoyl]pc) was evaluated in adult rat lung. fluorescence was observed at various times after intratracheal liposome instillation. fluorometric analysis of c12-nbd-pc in lung homogenate showed an initial rapid uptake followed by a more gradual uptake phase. microscopic observation of fixed lung slices showed c12-nbd-pc uptake in alveolar ty ... | 1994 | 8023961 |
| lr gold and lr white embedding of lung tissue for immunoelectron microscopy. | the immunogold technique in conjunction with lr gold or lr white embedding was used to localize different antigens in normal rat and mini pig lung tissues. lycopersicon esculentum and maclura pomifera lectins and diverse cytoskeletal antigens such as cytokeratins, beta-tubulin, smooth muscle-actin, tropomyosin and villin were labelled well. factor viii-related antigen was found in weibel-palade bodies of endothelial cells. a 10 kda clara cell protein was localized in clara cells. the morphologic ... | 1993 | 8135092 |
| morphological evidence of sperm maturation in the ampulla ductus deferentis of the bull. | the present lectin histochemical comparison of cauda epididymal and ampullary bovine sperm was conducted to investigate whether ampullary secretions are involved in altering the plasma-membrane glycoconjugates of sperm. a marked redistribution of glycoconjugates between sperm from these two regions was indeed revealed on the basis of changes in binding patterns of the following fluoroscein-isothiocyanate (fitc)-labelled lectins: wheat-germ agglutinin (wga), maclura pomifera agglutinin (mpa), gri ... | 1994 | 8137400 |
| lectin histochemistry on the skin of hairless descendants of mexican hairless dogs. | the skin of hairless descendants of mexican hairless dogs was investigated lectin-histochemically. haired dogs were also used for comparative study. in the epidermis of infant pups, lectin staining revealed marked differences between hairless and haired dogs. griffonia simplicifolia-i (gs-i) bound moderately to the cytoplasm of the stratum basale of hairless dogs, while no binding to that of haired dogs was observed. bauhinia purpurea (bpa) showed positive staining for the stratum spinosum and b ... | 1994 | 8171420 |