| growth-inhibitory effects of the red alga gelidium amansii on cultured cells. | the objective of this study was to investigate the effects of gelidium amansii, an edible red agar cultivated off the northeast coast of taiwan, on the growth of two lines of cancer cells, murine hepatoma (hepa-1) and human leukemia (hl-60) cells, as well as a normal cell line, murine embryo fibroblast cells (nih-3t3). the potential role of g. amansii on the induction of apoptosis was also examined. the results indicated that all extracts from g. amansii, including phosphate-buffered saline (pbs ... | 2004 | 14758029 |
| the immunostimulatory effects of hot-water extract of gelidium amansii via immersion, injection and dietary administrations on white shrimp litopenaeus vannamei and its resistance against vibrio alginolyticus. | the total haemocyte count (thc), phenoloxidase activity, and respiratory burst were examined when white shrimp litopenaeus vannamei were immersed in seawater (34 per thousand) containing hot-water extract of red alga gelidium amansii at 200, 400 and 600 mg l(-1), injected with hot-water extract at 4 and 6 microg g(-1) shrimp, and fed diets containing hot-water extract at 0, 0.5, 1.0 and 2.0 g kg(-1). these parameters increased significantly when shrimp were immersed in seawater containing hot-wa ... | 2007 | 17194604 |
| ethanol production from marine algal hydrolysates using escherichia coli ko11. | algae biomass is a potential raw material for the production of biofuels and other chemicals. in this study, biomass of the marine algae, ulva lactuca, gelidium amansii,laminaria japonica, and sargassum fulvellum, was treated with acid and commercially available hydrolytic enzymes. the hydrolysates contained glucose, mannose, galactose, and mannitol, among other sugars, at different ratios. the laminaria japonica hydrolysate contained up to 30.5% mannitol and 6.98% glucose in the hydrolysate sol ... | 2011 | 21640583 |
| isolation and characterization of a novel agarase-producing pseudoalteromonas spp. bacterium from the guts of spiny turban shells. | an agar-degrading bacterium was isolated from the guts of spiny turban shells. it was identified as a pseudoalteromonas species and named pseudoalteromonas sp. jybcl 1. the viscosity of the inoculated agar medium decreased by more than 60% after 20 h cultivation. the agarase produced by the isolate had optimal activities at 35 degrees c and ph 7. the enzyme had extremely strong resistance to ionic stress compared with other known agarases. its molecular mass was estimated at about 60 kda by sodi ... | 2011 | 21876371 |
| molecular cloning, characterization and enzymatic properties of a novel βeta-agarase from a marine isolate psudoalteromonas sp. ag52. | an agar-degrading pseudoalteromonas sp. ag52 bacterial strain was identified from the red seaweed gelidium amansii collected from jeju island, korea. a β-agarase gene which has 96.8% nucleotide identity to aeromonas β-agarase was cloned from this strain, and was designated as agaa. the coding region is 870 bp, encoding 290 amino acids and possesses characteristic features of the glycoside hydrolase family (ghf)-16. the predicted molecular mass of the mature protein was 32 kda. the recombinant β- ... | 2010 | 24031567 |
| molecular cloning, overexpression, and enzymatic characterization of glycosyl hydrolase family 16 β-agarase from marine bacterium saccharophagus sp. ag21 in escherichia coli. | an agar-degrading bacterium was isolated from red seaweed (gelidium amansii) on a natural seawater agar plate, and identified as saccharophagus sp. ag21. the β-agarase gene from saccharophagus sp. ag21 (agy1) was screened by long and accurate (la)-pcr. the predicted sequence has a 1,908 bp open reading frame encoding 636 amino acids (aa), and includes a glycosyl hydrolase family 16 (gh16) β-agarase module and two carbohydrate binding modules of family 6 (cbm6). the deduced aa sequence showed 93. ... | 2013 | 23711524 |
| evaluation of ethanol production and bioadsorption of heavy metals by various red seaweeds. | the objective of this study was to evaluate ethanol production and bioadsorption with four red seaweeds, gelidium amansii, gracilaria verrucosa, kappaphycus alvarezii and eucheuma denticulatum. to produce ethanol, thermal acid hydrolysis, enzymatic saccharification and fermentation was carried out. after pretreatment, 38.5, 39.9, 31.0 and 27.5 g/l of monosaccharides were obtained from g. amansii, g. verrucosa, k. alvarezii and e. denticulatum, respectively. ethanol fermentation was performed wit ... | 2016 | 26922420 |
| evolutionary engineering of saccharomyces cerevisiae for efficient conversion of red algal biosugars to bioethanol. | the aim of this work was to apply the evolutionary engineering to construct a mutant saccharomyces cerevisiae hj7-14 resistant on 2-deoxy-d-glucose and with an enhanced ability of bioethanol production from galactose, a mono-sugar in red algae. in batch and repeated-batch fermentations, hj7-14 metabolized 5-fold more galactose and produced ethanol 2.1-fold faster than the parental d452-2 strain. transcriptional analysis of genes involved in the galactose metabolism revealed that moderate relief ... | 2015 | 25804535 |
| ethanol production from the seaweed gelidium amansii, using specific sugar acclimated yeasts. | for the production of ethanol from seaweed as the source material, thermal acid hydrolysis and enzymatic saccharification were carried out for monosugars production of 25.5 g/l galactose and 7.6 g/l glucose using gelidium amansii. the fermentation was performed with pichia stipitis kctc 7228 or saccharomyces cerevisiae kccm 1129. when wild p. stipitis and s. cerevisiae were used, the ethanol productions of 11.2 g/l and 6.9 g/l were produced, respectively. the ethanol productions of 16.6 g/l and ... | 2014 | 24196166 |
| shewanella gelidii sp. nov., isolated from the red algae gelidium amansii, and emended description of shewanella waksmanii. | a novel gram-stain-negative, straight or slightly curved rod-shaped, non-spore-forming, facultatively anaerobic bacterium with a single polar flagellum, designated rzb5-4t, was isolated from a sample of the red algae gelidium amansii collected from the coastal region of rizhao, pr china (119.625° e 35.517° n). the organism grew optimally between 24 and 28 °c, at ph 7.0 and in the presence of 2-3 % (w/v) nacl. the strain required seawater or artificial seawater for growth, and nacl alone did not ... | 2016 | 27064664 |
| review on medicinal uses, pharmacological, phytochemistry and immunomodulatory activity of plants. | since ancient times, plants have been an exemplary source of medicine. researchers have discovered some important compounds from plants. the present work constitutes a review of the medicinal plants whose immunomodulant activity has been proven. we performed pubmed, embase, google scholar searches for research papers of medicinal plants having immunomodulant activity. medicinal plants used by traditional physicians or reported as having immunomodulant activity include acacia concocinna, camellia ... | 2017 | 25280022 |
| responses of cerebral gaba-containing cbm neuron to taste stimulation with seaweed extracts in aplysia kurodai. | aplysia kurodai distributed along japan feeds well on ulva pertusa but rejects gelidium amansii with distinctive patterned movements of the jaws and radula. on the ventral side of the cerebral m cluster, four cell bodies of higher order neurons that send axons to the buccal ganglia are distributed (cbm neurons). we have previously shown that the dopaminergic cbm1 modulates basic feeding circuits in the buccal ganglia for rejection by firing at higher frequency after application of the aversive t ... | 2005 | 16114014 |
| protective effect of the edible brown alga ecklonia stolonifera on doxorubicin-induced hepatotoxicity in primary rat hepatocytes. | as part of our efforts to isolate anti-hepatotoxic agents from marine natural products, we screened the ability of 14 edible varieties of korean seaweed to protect against doxorubicin-induced hepatotoxicity in primary rat hepatocytes. | 2014 | 24628384 |
| seaweed extracts as a potential tool for the attenuation of oxidative damage in obesity-related pathologies(1). | recent studies suggest that seaweed extracts are a significant source of bioactive compounds comparable to the dietary phytochemicals such as onion and tea extracts. the exploration of natural antioxidants that attenuate oxidative damage is important for developing strategies to treat obesity-related pathologies. the objective of this study was to screen the effects of seaweed extracts of 49 species on adipocyte differentiation and reactive oxygen species (ros) production during the adipogenesis ... | 2011 | 27021984 |
| gelidium elegans regulates the ampk-prdm16-ucp-1 pathway and has a synergistic effect with orlistat on obesity-associated features in mice fed a high-fat diet. | the incidence of obesity is rising at an alarming rate throughout the world and is becoming a major public health concern with incalculable social and economic costs. gelidium elegans (gens), also previously known as gelidium amansii, has been shown to exhibit anti-obesity effects. nevertheless, the mechanism by which gens is able to do this remains unclear. in the present study, our results showed that gens prevents high-fat diet (hfd)-induced weight gain through modulation of the adenosine mon ... | 2017 | 28358328 |
| proteomic analysis of the neurotrophic effect of gelidium amansii in primary cultured neurons. | gelidium amansii is an edible and economically important red alga consumed in south eastern asia. in previous studies, we reported that the ethanol extracts of g. amansii (gae) has promising modulatory activity with respect to the morphological and functional maturation of hippocampal neurons in culture. in this study, we show that the chloroform (chcl3) subfraction of gae and the ethyl acetate (etoac) fraction dose-dependently promoted neurite outgrowth, and their effects were comparable with t ... | 2017 | 28256936 |
| gelidium amansii extract ameliorates obesity by down-regulating adipogenic transcription factors in diet-induced obese mice. | in this study, we investigated whether gelidium amansii extract (gae) ameliorates obesity in diet-induced obese (dio) mice. | 2017 | 28194261 |
| hyper-thermal acid hydrolysis and adsorption treatment of red seaweed, gelidium amansii for butyric acid production with ph control. | optimal hyper-thermal (ht) acid hydrolysis conditions for gelidium amansii were determined to be 12% (w/v) seaweed slurry content and 144 mm h2so4 at 150 °c for 10 min. ht acid hydrolysis-treated g. amansii hydrolysates produced low concentrations of inhibitory compounds and adsorption treatment using 3% activated carbon. an adsorption time of 5 min was subsequently used to remove the inhibitory 5-hydroxymethylfurfural from the medium. a final maximum monosaccharide concentration of 44.6 g/l and ... | 2017 | 27878375 |
| enzymatic desulfation of the red seaweeds agar by marinomonas arylsulfatase. | agar and sulfated galactans were isolated from the red seaweeds gracilariopsis lemaneiformis and gelidium amansii. a previously purified arylsulfatase from marinomonas sp. fw-1 was used to remove sulfate groups in agar and sulfated galactans. after enzymatic desulfation, the sulfate content decreased to about 0.16% and gel strength increased about two folds. moreover, there was no difference between the dna electrophoresis spectrum on the gel of the arylsulfatase-treated agar and that of the com ... | 2016 | 27521846 |
| popular edible seaweed, gelidium amansii prevents against diet-induced obesity. | the popular edible seaweed, gelidium amansii is broadly used as food worldwide. to determine whether g. amansii extract (gae) has protective effects on obesity, mice fed a high-fat diet (hfd) treated with gae (1 and 3 %) were studied. after 12 weeks of gae treatment, body weight was greatly decreased in mice fed a high-fat diet. this effect could be due to decreased adipogenesis, as evidenced by the fact that gae suppressed adipogenic gene expression in adipocytes. in addition, blood glucose and ... | 2016 | 26911551 |
| marinagarivorans algicola gen. nov., sp. nov., isolated from marine algae. | novel agar-degrading, gram-staining-negative, motile, heterotrophic, facultatively anaerobic and pale yellow-pigmented bacterial strains, designated z1t and jl1, were isolated from marine algae gelidium amansii (lamouroux) and gracilaria verrucosa, respectively. growth of the isolates was optimal at 28-30 °c, ph 7.0-7.5 and 1-3% (w/v) nacl. both strains contained q-8 as the sole respiratory quinone. the major cellular fatty acids in strain z1t were c18:1 ω7c, c16:0 and summed feature 3 (c16:1 ω7 ... | 2016 | 26821539 |
| characterization of a novel alkaline arylsulfatase from marinomonas sp. fw-1 and its application in the desulfation of red seaweed agar. | a bacterial strain capable of hydrolyzing sulfate ester bonds of p-nitrophenyl sulfate (pnps) and agar was isolated from the coast area of qingdao, china. it was identified as marinomonas based on its 16s rrna gene sequence and named as marinomonas sp. fw-1. an arylsulfatase with a recovery of 13 % and a fold of 12 was purified to a homogeneity using ion exchange and gel filtration chromatographies. the enzyme was composed of a single polypeptide chain with the molecular mass of 33 kda estimated ... | 2015 | 26286088 |
| butyric acid production from red algae by a newly isolated clostridium sp. s1. | to produce butyric acid from red algae such as gelidium amansii in which galactose is a main carbohydrate, microorganisms utilizing galactose and tolerating inhibitors in hydrolysis including levulinic acid and 5-hydroxymethylfurfural (hmf) are required. | 2015 | 26026964 |
| aquimarina agarivorans sp. nov., a genome-sequenced member of the class flavobacteriia isolated from gelidium amansii. | a novel gram-stain-negative, facultatively anaerobic, rod-shaped, agar-digesting bacterial strain, designated hqm9t, was isolated from the surface of the marine red alga gelidium amansii collected from the intertidal zone of weihai, china. cells of hqm9t were 3.0-4.0 μm long and 0.2-0.3 μm wide and lacked flagella. the new isolate grew optimally at 28-30 °c, at ph 7.0-7.5, and in the presence of 2.5-3.0% nacl. the predominant cellular fatty acids were iso-c15 : 0 and iso-c17 : 0 3-oh. the sole m ... | 2015 | 25969473 |
| enhanced ethanol production by fermentation of gelidium amansii hydrolysate using a detoxification process and yeasts acclimated to high-salt concentration. | a total monosaccharide concentration of 59.0 g/l, representing 80.1 % conversion of 73.6 g/l total fermentable sugars from 160 g dw/l g. amansii slurry was obtained by thermal acid hydrolysis and enzymatic hydrolysis. subsequent adsorption treatment using 5 % activated carbon with an adsorption time of 2 min was used to prevent the inhibitory effect of 5-hydroxymethylfurfural (hmf) >5 g/l in the medium. ethanol production decreased with increasing salt concentration using c. tropicalis kctc 7212 ... | 2015 | 25627467 |
| efficient approach for bioethanol production from red seaweed gelidium amansii. | gelidium amansii (ga), a red seaweed species, is a popular source of food and chemicals due to its high galactose and glucose content. in this study, we investigated the potential of bioethanol production from autoclave-treated ga (atga). the proposed method involved autoclaving ga for 60min for hydrolysis to glucose. separate hydrolysis and fermentation processing (shf) achieved a maximum ethanol concentration of 3.33mg/ml, with a conversion yield of 74.7% after 6h (2% substrate loading, w/v). ... | 2015 | 25459813 |
| deoxy-liquefaction of three different species of macroalgae to high-quality liquid oil. | three species of macroalgae (ulva lactuca, laminaria japonica and gelidium amansii) were converted into liquid oils via deoxy-liquefaction. the elemental analysis, ftir and gc-ms results showed that the three liquid oils were all mainly composed of aromatics, phenols, alkanes and alkenes, other oxygen-containing compounds, and some nitrogen-containing compounds though there were some differences in terms of their types or contents due to the different constituents in the macroalgae feedstocks. t ... | 2014 | 25038506 |
| gelidium amansii promotes dendritic spine morphology and synaptogenesis, and modulates nmda receptor-mediated postsynaptic current. | neurotrophic factors are essential for the differentiation and maturation of developing neurons as well as providing survival support to the mature neurons. moreover, therapeutically neurotrophic factors are promising to reconstruct partially damaged neuronal networks in neurodegenerative diseases. in the previous study, we reported that the ethanol extract of an edible marine alga, gelidium amansii (gae) had shown promising effects in the development and maturation of both axon and dendrites of ... | 2014 | 24399252 |
| biotransformation of 5-hydroxymethylfurfural (hmf) by scheffersomyces stipitis during ethanol fermentation of hydrolysate of the seaweed gelidium amansii. | the seaweed, gelidium amansii, was fermented to produce bioethanol. optimal pretreatment condition was determined as 94 mm h2so4 and 10% (w/v) seaweed slurry at 121°c for 60 min. the mono sugars of 43.5 g/l with 57.4% of conversion from total carbohydrate of 75.8 g/l with g. amansii slurry 100g dcw/l were obtained by thermal acid hydrolysis pretreatment and enzymatic saccharification. g. amansii hydrolysate was used as the substrate for ethanol production by separate hydrolysis and fermentation ... | 2013 | 23714097 |
| extract from edible red seaweed (gelidium amansii) inhibits lipid accumulation and ros production during differentiation in 3t3-l1 cells. | gelidium (g.) amansii is a red alga widely distributed in the shallow waters around east asian countries. we investigated the effect of g. amansii on lipid accumulation and ros (reactive oxygen species) production in 3t3-l1 cells. g. amansii extracts dose-dependently inhibited lipid formation and ros generation in cultured cells. our results showed that anti-adipogenic effect of g. amansii was due to the reduction in mrna expressions of pparγ peroxisome proliferator-activated receptor-γ and ap2 ... | 2012 | 24471074 |
| behaviors of glucose decomposition during acid-catalyzed hydrothermal hydrolysis of pretreated gelidium amansii. | acid-catalyzed hydrothermal hydrolysis is one path to cellulosic glucose and subsequently to its dehydration end products such as hydroxymethyl furfural (hmf), formic acid and levulinic acid. the effect of sugar decomposition not only lowers the yield of fermentable sugars but also forms decomposition products that inhibit subsequent fermentation. the present experiments were conducted with four different acid catalysts (h(2)so(4), hno(3), hcl, and h(3)po(4)) at various acid normalities (0.5-2.1 ... | 2012 | 22522017 |
| the marine alga gelidium amansii promotes the development and complexity of neuronal cytoarchitecture. | neurotrophic factors are vital not only to support neuronal development but also to protect mature neurons from atrophy in neurodegenerative diseases. as an effort to explore natural sources that possess neurotrophic activity, we screened common marine algae for their neuritogenic activity in the developing rat hippocampal neurons in culture. of the 22 seaweed species examined, ethanol extracts of gelidium amansii (gae) exhibited potent neuritogenic activity, followed by undaria pinnatifida and ... | 2013 | 22438103 |
| use of gelidium amansii as a promising resource for bioethanol: a practical approach for continuous dilute-acid hydrolysis and fermentation. | a facile continuous method for dilute-acid hydrolysis of the representative red seaweed species, gelidium amansii was developed and its hydrolysate was subsequently evaluated for fermentability. in the hydrolysis step, the hydrolysates obtained from a batch reactor and a continuous reactor were systematically compared based on fermentable sugar yield and inhibitor formation. there are many advantages to the continuous hydrolysis process. for example, the low melting point of the agar component i ... | 2012 | 22261657 |
| two-stage acid saccharification of fractionated gelidium amansii minimizing the sugar decomposition. | two-stage acid hydrolysis was conducted on easy reacting cellulose and resistant reacting cellulose of fractionated gelidium amansii (f-ga). acid hydrolysis of f-ga was performed at between 170 and 200 °c for a period of 0-5 min, and an acid concentration of 2-5% (w/v, h2so4) to determine the optimal conditions for acid hydrolysis. in the first stage of the acid hydrolysis, an optimum glucose yield of 33.7% was obtained at a reaction temperature of 190 °c, an acid concentration of 3.0%, and a re ... | 2011 | 21963246 |
| red algae and their use in papermaking. | gelidialian red algae, that contain rhizoidal filaments, except the family gelidiellaceae were processed to make bleached pulps, which can be used as raw materials for papermaking. red algae consist of rhizoidal filaments, cortical cells usually reddish in color, and medullary cells filled with mucilaginous carbohydrates. red algae pulp consists of mostly rhizoidal filaments. red algae pulp of high brightness can be produced by extracting mucilaginous carbohydrates after heating the algae in an ... | 2010 | 20022488 |
| production of sugars and levulinic acid from marine biomass gelidium amansii. | this study focused on optimization of reaction conditions for formation of sugars and levulinic acid from marine algal biomass gelidium amansii using acid catalyst and by using statistical approach. by this approach, optimal conditions for production of sugars and levulinic acid were found as follows: glucose (reaction temperature of 139.4 degrees c, reaction time of 15.0 min, and catalyst concentration of 3.0%), galactose (108.2 degrees c, 45.0 min, and 3.0%), and levulinic acid (160.0 degrees ... | 2010 | 19830598 |
| the potential value of the seaweed ceylon moss (gelidium amansii) as an alternative bioenergy resource. | sea weed (ceylon moss) possesses comparable bioenergy production potential to that of land plants. ceylon moss has high content of carbohydrates, typically galactose (23%) and glucose (20%). we have explored the possibility of sodium chlorite in ceylon moss pretreatment that can ultimately increase the efficiency of enzymatic saccharification. in an acidic medium, chlorite generates clo(2) molecules that transform lignin into soluble compounds without any significant loss of carbohydrate content ... | 2009 | 19647997 |
| implications of mycosporine-like amino acid and antioxidant defenses in uv-b radiation tolerance for the algae species ptercladiella capillacea and gelidium amansii. | ultraviolet-b (uv-b) radiation (0.5, 1.0, 1.5, and 3.0wm(-2)) induced higher h(2)o(2) production and lipid peroxidation in alga gelidium amansii inhabiting in lower subtidal regions than upper subtidal alga ptercladiella capillacea. compared to g. amansii, mycosporine-like amino acid (maa) concentration in p. capillacea was higher and can be increased by 0.5-1.0wm(-2) uv-b, while carotenoid concentration was lower but also increased by 1.5-3.0wm(-2) uv-b. uv-b increased ascorbate concentration, ... | 2009 | 19036429 |
| antioxidative activities in some common seaweeds. | dietary antioxidants from plants are believed to help prevent aging and other diseases through radical scavenging activity. free radical scavenging activities by 1,1-diphenyl-2-picrylhydrazyl (dpph) assay and deoxyribose assay were ascertained among 27 species of common seaweeds using a sequential extraction method. the results indicated that, dpph scavenging activity existed in 15 species; gelidium amansii, gloiosiphonia capillaris, polysiphonia urceolata, sargassum kjellmanianum, desmarestia v ... | 1998 | 9950086 |