| a rapid and convenient method for purification and isolation of chlorophyll-a from porphyra yezoensis. | | 1978 | 100111 |
| suppression of 125i-uptake in mouse thyroid by seaweed feeding: possible preventative effect of dietary seaweed on internal radiation injury of the thyroid by radioactive iodine. | we conducted an animal experiment to determine how dietary seaweeds rich in iodine and dietary fibers suppress radioactive iodine uptake by the thyroid, using mice and four kinds of experimental diets, three with 1% or 2% powdered fronds of the kelp laminaria religiosa and 2% powdered laver porphyra yezoensis, and one with cellulose. iodine content of a hot-water extract of the kelp was 0.530 +/- 0.001%, and its dietary fiber (df) values were 52.8 +/- 1.2%. iodine in an extract of the laver was ... | 1992 | 1344008 |
| purification and identification of angiotensin i-converting enzyme inhibitors from the red alga porphyra yezoensis. | the potent part of the angiotensin i-converting enzyme (ace) inhibitory activity from porphyra yezoensis hydrolysate was fractionated by using ion-exchange and gel-filtration techniques. oral administration of the most potent inhibitory fraction (sp-i fraction, 200 mg/kg) to spontaneously hypertensive rats (shr) showed a hypotensive effect. using octadecylsilano column chromatography, the sp-i fraction was further separated into several peptides with potent inhibitory activities. the amino acid ... | 1998 | 9701638 |
| generation of 10,154 expressed sequence tags from a leafy gametophyte of a marine red alga, porphyra yezoensis. | a total of 10,154 5'-end expressed sequence tags (est) were established from the normalized and size-selected cdna libraries of a marine red alga, porphyra yezoensis. among the ests, 2140 were unique species, and the remaining 8014 were grouped into 1127 species. database search of the 3267 non-redundant ests by blast algorithm showed that the sequences of 1080 species (33.1%) have similarity to those of registered genes from various organisms including higher plants, mammals, yeasts, and cyanob ... | 2000 | 10907854 |
| feeding dried purple laver (nori) to vitamin b12-deficient rats significantly improves vitamin b12 status. | to clarify the bioavailability of vitamin b12 in lyophylized purple laver (nori; porphyra yezoensis), total vitamin b12 and vitamin b12 analogue contents in the laver were determined, and the effects of feeding the laver to vitamin b12-deficient rats were investigated. the amount of total vitamin b12 in the dried purple laver was estimated to be 54.5 and 58.6 (se 5.3 and 7.5 respectively) microg/100 g dry weight by lactobacillus bioassay and chemiluminescent assay with hog intrinsic factor respe ... | 2001 | 11430774 |
| an antifungal protein from the marine bacterium streptomyces sp. strain ap77 is specific for pythium porphyrae, a causative agent of red rot disease in porphyra spp. | a novel antifungal protein (sap) was found in the culture supernatant of a marine bacterium, streptomyces sp. strain ap77, and was purified. this protein was characterized by chemical, biochemical, and biological analyses. by using gel filtration, the molecular mass of sap was estimated to be 160 kda. structural analysis of sap by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization-time of flight mass spectrometry suggested that sap is compos ... | 2002 | 12039718 |
| increasing effect of nori on the fecal excretion of dioxin by rats. | the effects of nori (porphyra yezoensis), a kind of red alga, on the gastrointestinal absorption and reabsorption of 17 types of dioxin were investigated in male wistar rats. the rats were fed with 4 g of the control diet or 4 g of the nori diet containing a standard dioxin solution (233 ngteq/kg of body weight) for five consecutive days. in the group fed with the 10% nori diet, the fecal excretion of dioxin from days 1 to 5 was higher (p<0.01) than that of the control group by 5.5-fold for 2,3, ... | 2002 | 12506965 |
| telomerase inhibition by sulfoquinovosyldiacylglycerol from edible purple laver (porphyra yezoensis). | as high telomerase activity is detected in most cancer cells, telomerase represents a promising cancer therapeutic target. we investigated the inhibitory effect of sulfoquinovosyldiacylglycerol (sqdg), distributed in plants and seaweeds, on human telomerase in a cell-free system. sqdg inhibited telomerase activity dose-dependently with 50% inhibition at 22 microm, whereas monogalactosyldiacylglycerol and digalactosyldiacylglycerol did not, even at concentrations of 100 microm. moreover, we confi ... | 2004 | 15246557 |
| protein import pathways in 'complex' chloroplasts derived from secondary endosymbiosis involving a red algal ancestor. | heterokont algae such as diatoms and the raphidophyte heterosigma akashiwo and peridinin-containing dinoflagellates such as heterocapsa triquetra originally acquired their chloroplasts via secondary endosymbiosis involving a red algal endosymbiont and a eukaryote host, resulting in 'complex' chloroplasts surrounded by four and three membranes, respectively. the precursors of both heterokont and dinoflagellate chloroplast-targeted proteins are first inserted into the er with removal of an n-termi ... | 2005 | 15830125 |
| sodium, potassium-atpases in algae and oomycetes. | we have investigated the presence of k(+)-transporting atpases that belong to the phylogenetic group of animal na(+),k(+)-atpases in the pythium aphanidermatum stramenopile oomycete, the porphyra yezoensis red alga, and the udotea petiolata green alga, by molecular cloning and expression in heterologous systems. pcr amplification and search in est databases allowed one gene to be identified in each species that could encode atpases of this type. phylogenetic analysis of the sequences of these at ... | 2005 | 16167182 |
| algae acquire vitamin b12 through a symbiotic relationship with bacteria. | vitamin b12 (cobalamin) was identified nearly 80 years ago as the anti-pernicious anaemia factor in liver, and its importance in human health and disease has resulted in much work on its uptake, cellular transport and utilization. plants do not contain cobalamin because they have no cobalamin-dependent enzymes. deficiencies are therefore common in strict vegetarians, and in the elderly, who are susceptible to an autoimmune disorder that prevents its efficient uptake. in contrast, many algae are ... | 2005 | 16267554 |
| identification, expression, and evolutionary analyses of plant lipocalins. | lipocalins are a group of proteins that have been characterized in bacteria, invertebrate, and vertebrate animals. however, very little is known about plant lipocalins. we have previously reported the cloning of the first true plant lipocalins. here we report the identification and characterization of plant lipocalins and lipocalin-like proteins using an integrated approach of data mining, expression studies, cellular localization, and phylogenetic analyses. plant lipocalins can be classified in ... | 2005 | 16306142 |
| in vivo protective effect of porphyra yezoensis polysaccharide against carbon tetrachloride induced hepatotoxicity in mice. | to study the protective effect and possible mechanism of porphyra yezoensis polysaccharide (pyp) in hepatotoxicity mice, acute liver injury was successfully induced by injecting 0.2% carbon tetrachloride (ccl(4)) intraperitoneally. levels of alanine aminotransferase (alt) and aspartate aminotransferase (ast) in serum and liver homogenate, content of malondialdehyde (mda), activities of total superoxide dismutase (t-sod) in liver were measured by biochemical methods. liver index was calculated an ... | 2007 | 17869397 |
| molecular analysis of physiological responses to changes in nitrogen in a marine macroalga, porphyra yezoensis (rhodophyta). | the rhodophyte seaweed porphyra yezoensis, known more commonly world-wide as "nori", is an important commercial crop in japan. cultivation of nori in japan is often affected by outbreaks of "iroochi", a discoloration of the thalli due to a decrease in inorganic nutrients in the culture area that in turn decreases the amount of photosynthetic pigments in the thalli. treating thalli with inorganic nitrogen can reverse iroochi. in this paper, we report on the characterization of three p. yezoensis ... | 2008 | 18202829 |
| hualyzin, a symmetrical urea derivative isolated from penicillium herquei isolate ga4. | penicillium herquei isolate ga4 was isolated from the infected conchocelis of porphyra yezoensis. a large-scale fermentation using yeast extract sucrose medium and repeated chromatography afforded a new symmetrical urea derivative, hualyzin (1). the structure was determined by detailed nmr spectroscopic investigations and ms fragmentation analysis. | 2008 | 18479164 |
| fermentation properties of low-quality red alga susabinori porphyra yezoensis by intestinal bacteria. | susabinori (porphyra yezoensis), a red alga, is cultured and processed into a sheet-style dried food, nori, in japan. but significant amounts of cultured susabinori, which has a low protein content is discarded because of its low quality. the protein content of nori has been reported to be correlated inversely with the carbohydrate content. in this study, we examined the relationship between the protein content and the fermentation of nori by means of bfidobacteria. nori with a low protein conte ... | 2008 | 18603784 |
| chemoprotective effects of a protein from the red algae porphyra yezoensis on acetaminophen-induced liver injury in rats. | seaweeds contribute to the maintenance of health through their nutritional and medicinal properties. the effects of pyp, a 14 kda protein isolated from a hot-water extract of the marine alga porphyra yezoensis, on aap-induced liver injury in rats was evaluated. aap induced acute liver injury and aap-induced hepatotoxicity is the leading cause of liver failure. in this study, male sprague-dawley rats were assigned to one of three treatment groups: control, aap, or aap + pyp. compared with the con ... | 2008 | 18729249 |
| visualization of phosphoinositides via the development of the transient expression system of a cyan fluorescent protein in the red alga porphyra yezoensis. | phosphoinositides (pis) play important roles in signal transduction pathways and the regulation of cytoskeleton and membrane functions in eukaryotes. subcellular localization of individual pi derivative is successfully visualized in yeast, animal, and green plant cells using pi derivative-specific pleckstrin homology (ph) domains fused with a variety of fluorescent proteins; however, expression of fluorescent proteins has not yet been reported in any red algal cells. in the present study, we dev ... | 2009 | 19153794 |
| world's largest macroalgal bloom caused by expansion of seaweed aquaculture in china. | in late june 2008, just weeks before the opening of the beijing olympics, a massive green-tide occurred covering about 600km(2) along the coast of qingdao, host city for olympic sailing regatta. coastal eutrophication was quickly attributed with the blame by the international media and some scientists. however, we explored an alternative hypothesis that the cause of the green-tide was due to the rapid expansion of porphyra yezoensis aquaculture along the coastline over 180km away from qingdao, a ... | 2009 | 19261301 |
| ammonium induced expression of the red algal chloroplast gene ycf18, a putative homolog of the cyanobacterial nbla gene involved in nitrogen deficiency-induced phycobilisome degradation. | in cyanobacteria, nutrient deficiency-induced phycobilisome degradation is controlled by the nbla gene. red algae also have an nbla-related gene, ycf18, in their chloroplast genomes. to elucidate the role of ycf18, the expression pattern of ycf18 in a red alga, porphyra yezoensis, was investigated. ycf18 expression was low in nitrate medium, but was greatly promoted in ammonium medium. nitrogen starvation caused bleaching, but did not affect the expression of ycf18. the responses of ycf18 to nit ... | 2009 | 19270416 |
| [photodynamic effect of two kinds of phycobiliproteins on human liver cancer cell line smmc-7721 in vitro]. | we studied the effect of photodynamic therapy with phycobiliproteins on human liver cancer cells in vitro. with 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (mtt assay), we used two phycobiliproteins, r-phycoerythrin (r-pe) and c-phycocyanin (c-pc) prepared from porphyra yezoensis, to determine the killing rates and apoptosis rates of human liver cancer cells (smmc-7721) mediated by laser. when the concentration of r-pe was 120 mg/l, the survival rate of human liver cancer ... | 2009 | 19938487 |
| cloning and comparative studies of seaweed trehalose-6-phosphate synthase genes. | the full-length cdna sequence (3219 base pairs) of the trehalose-6-phosphate synthase gene of porphyra yezoensis (pytps) was isolated by race-pcr and deposited in genbank (ncbi) with the accession number ay729671. pytps encodes a protein of 908 amino acids before a stop codon, and has a calculated molecular mass of 101,591 daltons. the pytps protein consists of a tps domain in the n-terminus and a putative tpp domain at the c-terminus. homology alignment for pytps and the tps proteins from bacte ... | 2010 | 20714424 |
| description of maritalea porphyrae sp. nov. isolated from red algae (porphyra yezoensis) and transfer of zhangella mobilis to maritalea mobilis comb. nov. | three gram-negative, motile, aerobic bacteria were isolated from cultures of the marine red alga porphyra yezoensis. phylogenetic analysis based on 16s rrna gene sequences revealed that the novel strains were closely related to maritalea myrionectae (97.9 % similarity) and zhangella mobilis (96.2 %). z. mobilis had the closest sequence similarity of 97.9 % with m. myrionectae. the dna g+c contents of new isolates (49.4-50.0 mol%) were similar to those of m. myrionectae (52.3 mol%) and z. mobilis ... | 2011 | 21317275 |
| molecular characterization of adenosine 5'-monophosphate deaminase-the key enzyme responsible for the umami taste of nori (porphyra yezoensis ueda, rhodophyta). | the enzyme adenosine 5'-monophosphate deaminase (ampd, ec 3.5.4.6) catalyzes the conversion of adenosine 5'-monophosphate to inosine 5'-mononucleotide (imp). imp is generally known as the compound responsible for the umami taste of the edible red alga porphyra yezoensis ueda that is known in japan as nori. therefore, we suspect that ampd plays a key role in providing a favorable nori taste. in this study, we undertake the molecular characterization of nori-derived ampd. the nori ampd protein has ... | 2011 | 21519809 |
| purification, crystallization and preliminary x-ray analysis of phycocyanin and phycoerythrin from porphyra yezoensis ueda. | porphyra yezoensis is one of the most important and widely cultured seaweeds in china. phycobiliproteins exhibit excellent spectroscopic properties and play versatile roles in the biomedical, food, cosmetics and chemical synthetic dye industries. here, the purification and crystallization of phycoerythrin and phycocyanin, two phycobiliproteins extracted from p. yezoensis, are described. using a novel protocol including co-precipitation with ammonium sulfate and hydroxyapatite column chromatograp ... | 2011 | 21543866 |
| a glycoprotein from porphyra yezoensis produces anti-inflammatory effects in liposaccharide-stimulated macrophages via the tlr4 signaling pathway. | the purpose of this study was to investigate the antioxidant and anti-inflammatory effects of a glycoprotein isolated from the alga porphyra yezoensis in lps-stimulated raw 264.7 mouse macrophages. first, we extracted a novel material with antioxidant activity from p.-áyezoensis, confirmed by sds-page to be a glycoprotein, which we named p.-áyezoensis glycoprotein (pgp). pgp inhibited the production of no and ros and expression of inos, cox-2, tnf-+¦ and il-1+¦, which are involved in the pathoge ... | 2011 | 21701768 |
| Inhibitory effect of sulphated polysaccharide porphyran on nitric oxide production in lipopolysaccharide-stimulated RAW264.7 macrophages. | Porphyran, extracted from an edible red alga (Porphyra yezoensis), is a sulphated polysaccharide with a wide variety of biological activities including anti-tumour, antioxidant and immuno-modulating activities. In this study, we examined the effect of porphyran on nitric oxide (NO) production in mouse macrophage cell line RAW264.7 cells. Although no significant activity of porphyran to induce NO or tumour necrosis factor-a (TNF-a) production in RAW264.7 cells was observed at the concentration ra ... | 2012 | 21976706 |
| horizontal transfer of bacterial polyphosphate kinases to eukaryotes: implications for the ice age and land colonisation. | studies of online database(s) showed that convincing examples of eukaryote ppks derived from bacteria type ppk1 and ppk2 enzymes are rare and currently confined to a few simple eukaryotes. these enzymes probably represent several separate horizontal transfer events. retention of such sequences may be an advantage for tolerance to stresses such as desiccation or nutrient depletion for simple eukaryotes that lack more sophisticated adaptations available to multicellular organisms. we propose that ... | 2013 | 23738841 |
| discovery of a novel alginate lyase from nitratiruptor sp. sb155-2 thriving at deep-sea hydrothermal vents and identification of the residues responsible for its heat stability. | extremophiles are expected to represent a source of enzymes having unique functional properties. the hypothetical protein nis_0185, termed nitaly in this study, was identified as an alginate lyase-homolog protein in the genomic database of ϵ-proteobacteria nitratiruptor sp. sb155-2, which was isolated from deep-sea hydrothermal vents at a water depth of 1,000 m. among the characterized alginate lyases in the polysaccharide lyase family 7 (pl-7), the amino acid sequence of nitaly showed the highe ... | 2016 | 27231344 |
| chemoprotective effects of a recombinant protein from pyropia yezoensis and synthetic peptide against acetaminophen-induced chang liver cell death. | in the present study, the chemoprotective effects of recombinant pyropia yezoensis (p. yezoensis) protein 1 (pyp1) were examined in acetaminophen (apap)-treated chang liver cells. the analysis of p. yezoensis revealed the presence of both mature and immature variants of pyp1. pyp1s, designated as pyp1 (15 kda), pyp1-ac (12 kda) and pyp1-b (5 kda), were successfully expressed in escherichia coli, and their chemoprotective effects were then examined. in addition, a peptide of 11 residues (aleggkss ... | 2015 | 26096530 |
| chloroplast-encoded serotonin n-acetyltransferase in the red alga pyropia yezoensis: gene transition to the nucleus from chloroplasts. | melatonin biosynthesis involves the n-acetylation of arylalkylamines such as serotonin, which is catalysed by serotonin n-acetyltransferase (snat), the penultimate enzyme of melatonin biosynthesis in both animals and plants. here, we report the functional characterization of a putative n-acetyltransferase gene in the chloroplast genome of the alga laver (pyropia yezoensis, formerly known as porphyra yezoensis) with homology to the rice snat gene. to confirm that the putative pyropia yezoensis sn ... | 2015 | 25183745 |
| polaribacter porphyrae sp. nov., isolated from the red alga porphyra yezoensis, and emended descriptions of the genus polaribacter and two polaribacter species. | three gram-negative, non-motile, strictly aerobic strains, designated lnm-20(t), lcm-1 and lam-13, were isolated from thalli of the marine red alga porphyra yezoensis. phylogenetic analysis based on 16s rrna gene sequences revealed that the isolates were associated with the genus polaribacter in the family flavobacteriaceae and were most closely related to polaribacter dokdonensis dsw-5(t) (96.2 % 16s rrna gene sequence similarity) and polaribacter gangjinensis k17-16(t) (95.0 %). the dna g+c co ... | 2013 | 22904227 |
| isolation and functional characterization of an ammonium transporter gene, pyamt1, related to nitrogen assimilation in the marine macroalga pyropia yezoensis (rhodophyta). | ammonium and nitrate are the primary nitrogen sources in natural environments, and are essential for growth and development in photosynthetic eukaryotes. in this study, we report on the isolation and characterization of an ammonium transporter gene (pyamt1) which performs a key function in nitrogen (n) metabolism of pyropia yezoensis thalli. the predicted length of pyamt1 was 483 amino acids (aas). the aa sequence included 11 putative transmembrane domains and showed approximately 33-44% identit ... | 2016 | 27581686 |
| hypolipidaemic foods in china. | with the changes in dietary pattern in china, in recent years, hyperlipidaemia has become an important problem in the pathogenesis of chronic degenerative diseases, especially the cardio-cerebro-vascular diseases. from studies on laboratory animals and people with hyperlipidaemia, a number of hypolipidaemic foods and beverages have been identified, of relevance to traditional chinese food culture. their absence from the diet may, in part account for the increasing prevalence of hyperlipidaemia i ... | 1996 | 24394619 |
| a cellulose synthase (cesa) gene from the red alga porphyra yezoensis (rhodophyta)(1). | the cell walls of porphyra species, like those of land plants, contain cellulose microfibrils that are synthesized by clusters of cellulose synthase enzymes ("terminal complexes"), which move in the plasma membrane. however, the morphologies of the porphyra terminal complexes and the cellulose microfibrils they produce differ from those of land plants. to characterize the genetic basis for these differences, we have identified, cloned, and sequenced a cellulose synthase (cesa) gene from porphyra ... | 2009 | 27033658 |
| occurrence of anserine as an antioxidative dipeptide in a red alga, porphyra yezoensis. | to examine the antioxidative compounds of nonprotein amino acids in a red alga, porphyra yezoensis, an ethanol extract of the cultured thalli was fractionated with dowex columns. the basic fraction v showed strong antioxidative capacities with ferric thiocyanate and tbars measurements. in this basic fraction, histidine, 3-methylhistidine, carnosine, and anserine were detected beside ornithine, lysine, and arginine by amino acid analysis. the occurrence of carnosine and anserine suggests that the ... | 1998 | 27315933 |
| isolation of plastid ribosomes. | plastid ribosomes are responsible for a large part of the protein synthesis in plant leaves, green algal cells, and the vast majority in the thalli of red algae. plastid translation is necessary not only for photosynthesis but also for development/differentiation of plants and algae. while some isolated plastid ribosomes from a few green lineages have been characterized by biochemical and proteomic approaches, in-depth proteomics including analyses of posttranslational modifications and processi ... | 2017 | 27730617 |
| chemical and mass spectrometry characterization of the red alga pyropia yezoensis chemoprotective protein (pyp): protective activity of the n-terminal fragment of pyp1 against acetaminophen-induced cell death in chang liver cells. | in the present study, the chemical structure and chemoprotective activity of pyropia yezoensis protein (pyp) were investigated using sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis, automated protein sequencing, matrix-assisted laser desorption/ionization-quadrupole ion trap-time-of-flight mass spectrometry and a chemoprotective assay using a synthetic peptide. the pyp fraction was demonstrated to contain two proteins: pyp1 (10 kda, sds-resistant dimer) and pyp2 (10 kda). pyp1 is ... | 2015 | 25374159 |
| analysis of expressed sequence tags from the red alga griffithsia okiensis. | red algae are distributed globally, and the group contains several commercially important species. griffithsia okiensis is one of the most extensively studied red algal species. in this study, we conducted expressed sequence tag (ests) analysis and synonymous codon usage analysis using cultured g. okiensis samples. a total of 1,104 cdna clones were sequenced using a cdna library made from samples collected from dolsan island, on the southern coast of korea. the clustering analysis of these seque ... | 2007 | 18176538 |
| nadph oxidases in eukaryotes: red algae provide new hints! | the red macro-alga chondrus crispus is known to produce superoxide radicals in response to cell-free extracts of its green algal pathogenic endophyte acrochaete operculata. so far, no enzymes involved in this metabolism have been isolated from red algae. we report here the isolation of a gene encoding a homologue of the respiratory burst oxidase gp91(phox) in c. crispus, named ccrboh. this single copy gene encodes a polypeptide of 825 amino acids. search performed in available genome and est alg ... | 2006 | 16344959 |
| variety in excitation energy transfer processes from phycobilisomes to photosystems i and ii. | the light-harvesting antennas of oxygenic photosynthetic organisms capture light energy and transfer it to the reaction centers of their photosystems. the light-harvesting antennas of cyanobacteria and red algae, called phycobilisomes (pbss), supply light energy to both photosystem i (psi) and photosystem ii (psii). however, the excitation energy transfer processes from pbs to psi and psii are not understood in detail. in the present study, the energy transfer processes from pbs to pss in variou ... | 2017 | 28185041 |
| catenovulum maritimus sp. nov., a novel agarolytic gammaproteobacterium isolated from the marine alga porphyra yezoensis ueda (ast58-103), and emended description of the genus catenovulum. | a novel agarolytic, gram-stain negative, heterotrophic, facultatively anaerobic and pale-white pigmented bacterial strain, designated q1(t), was isolated from the marine alga porphyra yezoensis ueda (ast58-103) collected from the coastal area of weihai, china. the cells are motile by means of peritrichous flagella. the isolate requires nacl for growth, while seawater is not necessary, and growth occurs optimally at about 30-33 °c, in 1-3 % (w/v) nacl and at ph 7-7.5. strain q1(t) shows oxidase-p ... | 2015 | 26036673 |
| sulfitobacter pacificus sp. nov., isolated from the red alga pyropia yezoensis. | three gram-negative, aerobic, halophilic bacterial strains, scm2-10(t), scm-4, and 14c-6, were isolated from the algal medium of the red alga pyropia yezoensis (previously classified as porphyra yezoensis) grown in laboratory experiments. phylogenetic analysis based on 16s rrna gene sequences revealed that the organisms with high similarities to the three isolates are sulfitobacter geojensis mm-124(t) (98.7%), sulfitobacter noctilucicola nb-77(t) (98.7%), sulfitobacter noctilucae nb-68(t) (98.6% ... | 2015 | 25708049 |
| speciation in the marine crop pyropia yezoensis (bangiales, rhodophyta). | in the marine red alga pyropia yezoensis, commonly known in japan as nori, sympatric occurrence of two cryptic species pyropia sp. 2 and pyropia sp. 3 on the same rock in a natural habitat has been confirmed by molecular analysis and detailed morphological observations. to confirm whether pyropia sp. 2 and pyropia sp. 3 were reproductively isolated in the sympatric population, 170 blades that had previously been studied using a maternally inherited plastid marker were examined with a nuclear gen ... | 2014 | 26988643 |
| sulfitobacter porphyrae sp. nov., isolated from the red alga porphyra yezoensis. | gram-stain-negative, aerobic, halophilic bacteria, designated scm-1(t), lcm10-1 and ctbl-b-147, were isolated from modified half-strength swm-iii medium, pes medium and thalli after laboratory cultivation of a red alga, porphyra yezoensis. phylogenetic analysis of 16s rrna gene sequences indicated that the new isolates were affiliated to the genus sulfitobacter of the class alphaproteobacteria, and the 16s rrna gene sequence similarity of the new isolates with the closest related species, sulfit ... | 2014 | 24096354 |
| algimonas ampicilliniresistens sp. nov., isolated from the red alga porphyra yezoensis, and emended description of the genus algimonas. | three strains (14a-2-7(t), 14a-3-1 and 14a-3) of gram-stain-negative, prosthecate, motile bacteria were isolated from an algal medium supplemented with 10 mg ampicillin l(-1) (w/v), in which the red alga porphyra yezoensis had been cultured. based on the 16s rrna gene sequence analysis, the three isolates formed a cluster with the genus algimonas of the family hyphomonadaceae. the sequences of the three isolates had high similarity with those of algimonas porphyrae 0c-2-2(t) (97.6 % similarity) ... | 2013 | 23852154 |
| molecular identification of green algae from the rafts based infrastructure of porphyra yezoensis. | to provide more information on the origin of the ulva prolifera bloom in qingdao sea area in china from 2007 to 2011, the diversity of green algae growing on the rafts of porphyra yezoensis on the coast in jiangsu province was investigated based on its, rbcl and 5s sequences. eighty-four of green algal samples from various sites and cruises in 2010 and 2011 were collected. according to its and rbcl sequences, samples from the rafts of p. yezoensis fell into four clades: ulva linza-procera-prolif ... | 2012 | 22858010 |
| algimonas porphyrae gen. nov., sp. nov., a member of the family hyphomonadaceae, isolated from the red alga porphyra yezoensis. | three gram-negative, stalked, motile bacteria, designated 0c-2-2(t), 0c-17 and lnm-3, were isolated from the red alga porphyra yezoensis. 16s rrna gene sequence analysis revealed that the three novel strains belonged to the family hyphomonadaceae, and were closely related to litorimonas taeanensis g5(t) (96.5 % 16s rrna gene sequence similarity) and hellea balneolensis 26iii/a02/215(t) (94.3 %). the dna g+c contents of the novel isolates (58.5-60.2 mol%) were clearly distinguished from those of ... | 2013 | 22427444 |
| visualization of nuclear localization of transcription factors with cyan and green fluorescent proteins in the red alga porphyra yezoensis. | transcription factors play a central role in expression of genomic information in all organisms. the objective of our study is to analyze the function of transcription factors in red algae. one way to analyze transcription factors in eukaryotic cells is to study their nuclear localization, as reported for land plants and green algae using fluorescent proteins. there is, however, no report documenting subcellular localization of transcription factors from red algae. in the present study, using th ... | 2010 | 19593603 |
| taxonomy, molecular phylogeny, and ultrastructural morphology of olpidiopsis porphyrae sp. nov. (oomycetes, straminipiles), a unicellular obligate endoparasite of bangia and porphyra spp. (bangiales, rhodophyta). | olpidiopsis porphyrae sp. nov., a marine oomycete endoparasite that infects the commercially cultivated red alga porphyra yezoensis, is described and its phylogenetic position based on molecular data and ultrastructural morphology is discussed. o. porphyrae infects the host porphyra by means of encysted zoospores. spherical-shaped holocarpic thalli develop within the cytoplasm of its algal host, which produce monoplanetic, subapically biflagellate zoospores. the characteristic features of this i ... | 2008 | 18308530 |
| isolation, antimicrobial activity, and metabolites of fungus cladosporium sp. associated with red alga porphyra yezoensis. | cladosporium sp. isolate n5 was isolated as a dominant fungus from the healthy conchocelis of porphyra yezoensis. in the re-infection test, it did not cause any pathogenic symptoms in the alga. twenty-one cultural conditions were chosen to test its antimicrobial activity in order to obtain the best condition for large-scale fermentation. phenylacetic acid, p-hydroxyphenylethyl alcohol, and l-beta-phenyllactic acid were isolated from the crude extract as strong antimicrobial compounds and they ar ... | 2008 | 18214603 |