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biochemical and serological characterization of carnobacterium spp. isolated from farmed and natural populations of striped bass and catfish.a comparative analysis of the phenotypic and serological properties of carnobacterium strains associated with mortalities of cultured striped bass and channel catfish and the properties of isolates from wild brown bullhead catfish in the chesapeake bay area in maryland was conducted. all of the strains were gram-positive, facultatively anaerobic, nonmotile, non-spore-forming rods occurring singly or in short chains. they did not produce cytochrome oxidase or catalase, did not reduce nitrate, fai ...19911781676
a numerical taxonomic study of lactic acid bacteria isolated from irradiated pork and chicken packaged under various gas atmospheres.ninety-four gram-positive, catalase-negative bacteria, isolated from pork and chicken that had been packed in modified atmospheres and irradiated to 1.75 and 2.5 kgy respectively, were studied. the majority of the strains were lactobacillus saké. numerical taxonomy, with the group average clustering strategy, revealed the existence of six clusters at the 85% similarity level. the largest, cluster 1, contained 78 (83%) of the test strains along with three lact. sak'e strains. cluster 2 contained ...19912055792
chitosan-colloidal gold complexes as polycationic probes for the detection of anionic sites by transmission and scanning electron microscopy.a new cationic colloidal gold complex has been developed for ultrastructural localization of cell surface anionic sites by transmission and scanning electron microscopy. the marker is prepared by labelling gold particles of suitable sizes (6 to 70 nm in diameter) with chitosan, a polymer of beta (1----4)-linked d-glucosamine. using human red blood cells as a model, chitosan-gold complexes were shown to be specific for anionic sites and at ph 2 for sialic acid residues. the binding capacity of co ...19883063695
attributes of microbial associations of meat growing as xenic batch cultures in a meat juice at 4 degrees c.strains of gram-positive (carnobacterium piscicola, leuconostoc mesenteroides subsp. mesenteroides, brochothrix thermosphacta) and gram-negative (pseudomonas fragi and hafnia alvei) bacteria isolated from minced lamb packaged under a modified atmosphere were cultivated in a meat (lamb) juice at 4 degrees c. carbohydrates were catabolised in the order glucose > glucose 6-phosphate during the development of the population. under an atmosphere enriched with carbon dioxide the gram-negative portion ...19957488524
heterologous expression of the lactacin f peptides by carnobacterium piscicola lv17.the lactacin f complex, composed of lafa and lafx peptides, is produced by lactobacillus johnsonii vpi 11088 and is active against five other lactobacillus species and enterococcus faecalis. the genetic determinants encoding the lactacin f complex are organized in a 1-kb polycistronic operon which comprises three genes, lafa, lafx, and orfz (encoding the putative immunity protein). the lafa and lafx genes encode the bacteriocin precursors with n-terminal extensions characterized by a gly-gly-1*x ...19957747957
purification and cloning of piscicolin 61, a bacteriocin from carnobacterium piscicola lv61.piscicolin 61, a bacteriocin produced by carnobacterium piscicola lv61, inhibits the growth of strains of carnobacterium, lactobacillus, and enterococcus. the bacteriocin was purified to homogeneity by ammonium sulfate precipitation and sequential hydrophobic interaction and reversed-phase chromatography. the n-terminal amino acid sequence of piscicolin 61 was determined by edman degradation. the plasmid-located structural gene encoding piscicolin (psc61) was cloned and sequenced. it encoded a p ...19947764997
carnocin ui49, a potential biopreservative produced by carnobacterium piscicola: large scale purification and activity against various gram-positive bacteria including listeria sp.this paper describes a simple purification method for the purification of carnocin ui49, a potential biopreservative produced by carnobacterium piscicola ui49. the protocol was also applicable for the isolation of nisin z, which is a biopreservative produced by lactococcus lactis sik-83. the protocol consists of only two purification steps, xad chromatography and cation exchange chromatography. it is quick, easy, and can be used for large scale purification of these lantibiotics. the bactericida ...19938110598
development of a specific biotinylated dna probe for the detection of renibacterium salmoninarum.a specific dna probe for the identification of renibacterium salmoninarum, the causative agent of bacterial kidney disease (bkd), was developed from one of 3 clones prs47, prs49, and prs26 of 5.1 kb, 5.3 kb, and 11.3 kb, respectively. the biotinylated prs47/bamhi insert probe was tested on 3 dilutions of dna extracted from 3 strains of r. salmoninarum and from 1 strain each of arthrobacter protophormiae, aeromonas salmonicida, corynebacterium aquaticum, carnobacterium piscicola, listonella angui ...19958548693
purification and amino acid sequences of piscicocins v1a and v1b, two class iia bacteriocins secreted by carnobacterium piscicola v1 that display significantly different levels of specific inhibitory activity.two bacteriocins produced by carnobacterium piscicola v1 were purified and characterized. piscicocin v1a (molecular mass = 4,416 da) and piscicocin v1b (molecular mass = 4,526 da) are nonlantibiotic, small, heat-stable antibacterial peptides. piscicocin v1b is identical to carnobacteriocin bm1, while piscicocin v1a is a new bacteriocin. its complete sequence of 44 amino acid residues has been determined. piscicocin v1a belongs to the class iia bacteriocins having the consensus ygngv motif. these ...19968953713
antibacterial activity of selected fatty acids and essential oils against six meat spoilage organisms.the antibacterial activity of selected fatty acids and essential oils was examined against two gram-negative (pseudomonas fluorescens and serratia liquefaciens) and four gram-positive (brochothrix thermosphacta, carnobacterium piscicola, lactobacillus curvatus, and lactobacillus sake) bacteria involved in meat spoilage. various amounts of each preservative were added to brain heart infusion or mrs (deman, rogosa and sharpe) agars, and the minimum inhibitory concentration was determined for each ...19979310850
evaluation of the role of carnobacterium piscicola in spoilage of vacuum- and modified-atmosphere-packed cold-smoked salmon stored at 5 degrees c.the microflora on spoiled cold-smoked salmon often consists of a mixture of lactic acid bacteria (lab) and gram-negative bacteria. to elucidate the role of the different groups, a storage trial was carried out in which nisin and co2 were used for the selective inhibition of the two bacterial groups. the shelf-life of vacuum-packed cold-smoked salmon, recorded by sensory evaluation, was four weeks at 5 degrees c and the microflora was composed of lab (10(6)-10(7) cfu/g) with an associate gram-neg ...19989553794
study of the microbial ecology of cold-smoked salmon during storage at 8 degrees c.microbiological, chemical and sensory changes in cold-smoked salmon were studied during 5 weeks of vacuum storage at 8 degrees c. the aerobic 20 degrees c viable count reached its maximum level after 6 days (3 x 10(6) cfu g-1) however, the shelf-life of the product was estimated to be 2 or 3 weeks by the panellists, confirming that there is no correlation between those two factors. acid, pungent, sour and rancid odours and flavours and pasty texture were the main spoilage characteristics. trimet ...19989562883
enumeration of carnobacterium divergens v41, carnobacterium piscicola v1 and lactobacillus brevis lb62 by in situ hybridization-flow cytometry.the specific detection and enumeration of lactobacillus brevis lb62, carnobacterium divergens v14 and carnobacterium piscicola vi were studied by in situ hybridization-flow cytometry. the method was performed on the exponential growth phase with three probes targeting 16s rrna labelled with fluorescein isothicyanate (fitc): eub338 probe universal for eubacteria, lb probe specific for lact. brevis and cb probe specific for the genus carnobacterium. eub338 was used to determine the permeabilizatio ...19989830150
the effect of diet on aerobic bacterial flora associated with intestine of arctic charr (salvelinus alpinus l.).in order to extend the knowledge on the possible effect of diet on the gastrointestinal microbial community of fish, arctic charr (salvelinus alpinus l.) were fed diets containing high (23.7%) and low (6.4%) levels of carbohydrate. the number of viable aerobic and facultative aerobic bacteria associated with the digestive tract were not influenced by dietary regimen. a wide range of bacterial species was isolated, and the predominant bacterial species of both rearing groups were identified as st ...199910030010
the effect of dietary fatty acids on lactic acid bacteria associated with the epithelial mucosa and from faecalia of arctic charr, salvelinus alpinus (l.).arctic charr, salvelinus alpinus (l.), held in fresh water, were fed four experimental diets containing different polyunsaturated fatty acids (pufa). in addition, one group fed a diet containing only coconut oil as sole lipid source served as control. the population of aerobic heterotrophic bacteria associated with the epithelial mucosa and the faecalia was estimated using the dilution plate technique. generally, the population level of adherent bacteria increased along the digestive tract (stom ...19989830121
enhancement of bacteriocin production by carnobacterium divergens as7 in the presence of a bacteriocin-sensitive strain carnobacterium piscicola.the effect of carnobacterium piscicola in the growth medium of carnobacterium divergens on divercin production was studied. c. piscicola cultures were added in the form of living cultures, thermally inactivated cultures and pretreated autolyzed cultures. each form was applied as whole culture comprising growth medium with cells, culture supernatants and cell pellets. it was found that the divercin-sensitive bacterium enhanced significantly the divercin production by c. divergens. the highest sti ...19989706799
detection and partial characterization of a bacteriocin produced by carnobacterium piscicola 213.blis 213, is a bacteriocin-like inhibitory substance produced by carnobacterium piscicola 213. it is active against carnobacterium, enterococcus and listeria spp. no activity was observed against tested lactobacillus, lactococcus, leuconostoc and pediococcus strains, nor against gram-negative bacteria. the blis 213 activity was inactivated by several proteolytic enzymes. it was heat resistant (121 degrees c for 20 min), and stable over a ph range of 2-8. activity was determined by a dilution mic ...19979281850
transcriptional analysis and regulation of carnobacteriocin production in carnobacterium piscicola lv17.bacteriocin production by carnobacterium piscicola lv17 (carnobacteriocin, cbn) depends on the level of inoculation when grown in liquid medium. with an inoculum of > or = 10(6) colony-forming units per ml (cfu/ml), bacteriocin production is observed during exponential growth, whereas with < or = 10(4) cfu/ml no bacteriocin is detected even when the culture has reached stationary phase. using pure bacteriocins, it was demonstrated that bacteriocin production is autoregulated. to understand how b ...19979133602
effect of amino acid substitutions on the activity of carnobacteriocin b2. overproduction of the antimicrobial peptide, its engineered variants, and its precursor in escherichia coli.carnobacteriocin b2, a 48-amino acid antimicrobial peptide containing a ygngv motif that is produced by the lactic acid bacterium carnobacterium piscicola lv17b, was overexpressed as fusion with maltose-binding protein in escherichia coli. this fusion protein was cleaved with factor xa to allow isolation of the mature bacteriocin that was identical in all respects to that obtained from c. piscicola. similar methodology permitted production of the precursor precarnobacteriocin b2 (cbnb2p), which ...19979013580
production of histamine and tyramine by lactic acid bacteria isolated from vacuum-packed sugar-salted fish.the incidence of histamine- or tyramine-producing lactic acid bacteria was examined in several products of vacuum-packed sugar-salted fish (salmon, halibut, mackerel). no histamine-producing isolates were observed, whereas the majority of tyramine-producing isolates were identified as carnobacterium spp. these organisms were shown to be important members of the microbial flora during storage of vacuum-packed sugar-salted salmon at 5 degrees c. the amount of tyramine produced was reduced by lower ...19948005830
characteristics of vagococcus salmoninarum isolated from diseased salmonid fish.isolates of the salmonid pathogen vagococcus salmoninarum were recovered from atlantic salmon, rainbow trout and brown trout with peritonitis. the phenotypes of these isolates and the type strain of vag. salmoninarum ncfb 2777 were determined by morphological, biochemical and physiological tests and whole cell protein profiles by sds-page. there was a high level of phenetic similarity between the salmonid isolates and the type strain. the species forms short gram-positive rods, hydrolyses l-pyrr ...19947961194
lactic acid bacteria associated with the digestive tract of atlantic salmon (salmo salar l.).the present study reports the effect of excessive handling stress and starvation on the lactic acid bacteria associated with the digestive tract of atlantic salmon (salmo salar l.). a relatively low population level (approximately 2 x 103 bacteria per gram wet tissue) of viable adherent heterotrophic bacteria was associated with the digestive tract (foregut, midgut and hindgut). of the 752 bacterial isolates isolated from diet, water and the digestive tract, 201 isolates belonged to the carnobac ...200010971765
characterization of the protein conferring immunity to the antimicrobial peptide carnobacteriocin b2 and expression of carnobacteriocins b2 and bm1.cloning of a 16-kb dna fragment from the 61-kb plasmid of carnobacterium piscicola lv17b into plasmidless c. piscicola lv17c restores the production of the plasmid-encoded carnobacteriocin b2 and the chromosomally-encoded carnobacteriocin bm1 and restores the immune phenotype. this fragment also has sufficient genetic information to allow the expression of carnobacteriocin b2 and its immunity in a heterologous host. the gene locus (cbib2) responsible for immunity to carnobacteriocin b2 is locate ...19957868585
numerical taxonomy of psychrotrophic lactic acid bacteria from prepacked meat and meat products.ninety-four strains of lactic acid bacteria isolated from refrigerated, prepacked meat and meat products were together with 59 reference strains of brochothrix, lactobacillus, leuconostoc, pediococcus and streptococcus phenotypically classified, using 96 unit characters. data were examined using simple matching (ssm) or jaccard coefficient (sj), and unweighted pair group algorithm with arithmetic averages. twenty-three clusters with two or more members were defined at the 84% ssm-similarity leve ...19883178187
isolation and properties of a bacteriocin-producing carnobacterium piscicola isolated from fish.a facultative psychrotrophic lactic acid bacterium isolated from fresh fish was identified as carnobacterium piscicola on the basis of carbohydrate utilization, g + c content and 16s rna analysis. its bacteriocin, designated carnocin ui49, is produced during the mid-exponential phase of growth at temperatures between 15 degrees c and 34 degrees c. carnocin ui49 is active against a large number of closely-related lactic acid bacteria including carnobacteria, lactobacilli, pediococci and lactococc ...19921429307
identification and characterization of carnobacteria associated with the gills of atlantic salmon (salmo salar l.).using the surface plate technique, the population level of aerobic bacteria, occurring in the gills of atlantic salmon (salmo salar l.), was determined to be approximately 3 x 10(4) g(-1). of 100 isolates investigated, 58 were gram-negative. out of the 42 gram-positive isolates, 26 belonged to the carnobacteria, of which ten were further identified on the basis of 16s rdna sequence analysis and aflp fingerprinting. all were identified as carnobacterium piscicola-like. these carnobacteria strains ...200011249022
modulation of crassostrea virginica hemocyte reactive oxygen species production by listonella anguillarum.luminol- and lucigenin-augmented chemiluminescence (cl) were used to evaluate the ability of listonella (formerly vibrio) anguillarum to stimulate the production of reactive oxygen species (ros) by crassostrea virginica hemocytes. whereas heat-killed l. anguillarum stimulated hemocyte cl in the lucigenin system, viable l. anguillarum did not. neither viable nor heat-killed bacteria stimulated hemocyte production of luminol cl. metabolically active l. anguillarum generated ros, as indicated by lu ...19979303272
characterisation of volatile compounds produced by bacteria isolated from the spoilage flora of cold-smoked salmon.this study investigated the volatile compounds produced by bacteria belonging to nine different bacterial groups: lactobacillus sake, l. farciminis, l. alimentarius, carnobacterium piscicola, aeromonas sp., shewanella putrefaciens, brochothrix thermosphacta, photobacterium phosphoreum and enterobacteriaceae isolated from cold-smoked salmon. each bacterial group was represented by several strains. in addition, combinations of the groups were examined as well. sterile blocks of cold-smoked salmon ...200111428576
factors affecting production of an antilisterial bacteriocin by carnobacterium piscicola strain a9b in laboratory media and model fish systems.to investigate factors influencing bacteriocin production and bacteriocin stability of the bioprotective culture carnobacterium piscicola strain a9b.200111556917
a two-component signal-transduction cascade in carnobacterium piscicola lv17b: two signaling peptides and one sensor-transmitter.in the lactic acid bacterium carnobacterium piscicola lv17b a peptide-pheromone dependent quorum-sensing mode is involved in the regulation of bacteriocin production. bacteriocin cb2 was identified as an environmental signal that induces bacteriocin production. here, we demonstrate that a second 24 amino acid peptide (cs) also induces bacteriocin production. transcription activation of several carnobacteriocin operons is triggered by cb2 or cs via a two-component signal transduction system compo ...200111587787
expression of the antimicrobial peptide carnobacteriocin b2 by a signal peptide-dependent general secretory pathway.carnobacteriocin b2 is a well-characterized class ii bacteriocin produced by a 61-kb plasmid from carnobacterium piscicola lv17. export of this bacteriocin is dependent on specific abc (atp-binding cassette) secretion proteins. divergicin a is a strongly hydrophobic narrow-spectrum bacteriocin produced by a 3.4-kb plasmid from carnobacterium divergens lv13. predivergicin a contains a signal peptide and utilizes the general secretary pathway for export (r. w. worobo, m. j. van belkum, m. sailer, ...19968900000
bacteriocin production by carnobacterium piscicola lv 61.carnobacterium piscicola lv 61 produces a bacteriocin designated piscicolin 61, that is heat resistant, active over a wide ph range and inactivated by alpha-chymotrypsin, pepsin, trypsin and papain. it is effective against strains of the genera carnobacterium, enterococcus and listeria. other lactic acid bacteria tested were less sensitive or resistant to piscicolin. it is produced at temperatures from 1 to 30 degrees c. maximum bacteriocin activity was detected after the culture had entered the ...19938312140
effect of the bacteriocin carnocin cp5 and of the producing strain carnobacterium piscicola cp5 on the viability of listeria monocytogenes atcc 15313 in salt solution, broth and skimmed milk, at various incubation temperatures.carnobacterium piscicola cp5, isolated from french mould-ripened soft-cheese, produced a bacteriocin named carnocin cp5 in a wide range of incubation temperatures, from 4 degrees c to 30 degrees c. the ability of a crude bacteriocin, of a partially-purified form, and of the producer strain to inhibit growth of listeria monocytogenes atcc 15313 was examined in salt solution, broth and skimmed milk between 4 degrees c and 30 degrees c. when carnocin cp5 was added to a l. monocytogenes atcc 15313 c ...19948074969
change in the composition of the microflora on vacuum-packaged beef during chiller storage.five fresh beefcut samples were divided into 10 pieces, respectively, vacuum-packaged and stored at 2 degrees c for up to 6 weeks. the average ph values of five pieces from five beef samples were 5.62 +/- 0.04 at the start of storage and 5.12 +/- 0.07 after 6 weeks of storage. the pieces were homogenized, diluted and cultivated at weekly intervals on glucose-blood-liver (bl), de man rogosa and sharpe (mrs) and trypticase soya (ts) agars at 7 degrees c for 10-14 days. from plates with 30-300 colo ...200211929174
microbial spoilage and formation of biogenic amines in fresh and thawed modified atmosphere-packed salmon (salmo salar) at 2 degrees c.to evaluate the microbial spoilage, formation of biogenic amines and shelf life of chilled fresh and frozen/thawed salmon packed in a modified atmosphere and stored at 2 degrees c.200211966922
role of acetate in production of an autoinducible class iia bacteriocin in carnobacterium piscicola a9b.carnobacterium piscicola strain a9b isolated from cold smoked salmon inhibits growth of the food-borne pathogen listeria monocytogenes partly due to the production of a proteinaceous compound (l. nilsson, l. gram, and h. h. huss. j. food prot. 62:336-342, 1999). the purpose of the present study was to purify the compound and describe factors affecting its production, with particular emphasis on food-relevant factors. amino acid sequencing showed that the compound is a class iia bacteriocin with ...200211976095
characteristics and genetic determinant of a hydrophobic peptide bacteriocin, carnobacteriocin a, produced by carnobacterium piscicola lv17a.carnobacteriocin a is a hydrophobic nonlantibiotic bacteriocin that is detected early in the growth cycle of carnobacterium piscicola lv17a and encoded by a 49 mda plasmid. the bacteriocin was purified using hydrophobic interaction and gel filtration chromatography, and reversed-phase hplc. three different active peaks (a1, a2 and a3) were detected, but the purified samples had identical n-terminal amino acid sequences for the first 15 amino acids as determined by edman degradation analysis. onl ...19948012574
effect of growth of selected lactic acid bacteria on storage life of beef stored under vacuum and in air.the effect of growth of different types of lactic acid bacteria (lab) on the storage life of normal ph beef was determined anaerobically (under vacuum) and aerobically. four lab from meat were inoculated separately onto sterile slices of lean beef. inoculated samples were stored anaerobically at 2 degrees c for 10 weeks or stored aerobically in an oxygen permeable film at 7 degrees c for 10 days, with and without previous storage under vacuum at 2 degrees c. the lab strains used were carnobacter ...19957577360
comparative studies of immunity proteins of pediocin-like bacteriocins.genes encoding pediocin-like bacteriocins are usually co-transcribed with a gene encoding a cognate immunity protein. to investigate the functionality and specificity of immunity proteins, immunity genes belonging to the bacteriocins curvacin a, enterocin a, enterocin p, leucocin a, pediocin pa-1 and sakacin p, as well as a putative immunity gene, orfy, were expressed in three bacteriocin-sensitive lactic acid bacteria (lactobacillus sake, carnobacterium piscicola and enterococcus faecalis). the ...200212427956
nucleic acid relatedness studies on the genus carnobacterium and related taxa.none of the species carnobacterium piscicola, c. divergens, c. mobile or c. gallinarum showed significant dna-dna homology between themselves and other lactic acid bacteria [corrected]. nevertheless, the carnobacterium species were found to belong to the same ribosomal rna homology cluster. the species in this cluster were distant from the other bacteria tested.19892482862
plasmid-associated bacteriocin production by a strain of carnobacterium piscicola from meat.carnobacterium piscicola lv17 isolated from vacuum-packed meat produces bacteriocin(s) that is active against closely related lactic acid bacteria, enterococcus spp., and a strain of listeria monocytogenes but not against gram-negative bacteria. the bacteriocin has a bactericidal mode of action, is heat resistant, and is stable over a wide range of ph but is inactivated by proteolytic enzymes. sensitive and resistant cells were shown to adsorb the bacteriocin, but cell death depended on contact ...19902403256
purification and characterization of brochocin a and brochocin b(10-43), a functional fragment generated by heterologous expression in carnobacterium piscicola.brochothrix campestris atcc 43754 produces a heat-stable, two-component, nonlantibiotic, class iib bacteriocin, brochocin c (brcc), that is active against a broad range of gram-positive bacteria, including spores of clostridium botulinum. an improved purification method was developed for brcc, in which n-butanol and chloroform extraction are used. mass spectral characterization of the two components, brochocin a (brca) and brochocin b (brcb), showed that both components are excreted into the med ...200312620816
16s rrna sequence determination for members of the genus carnobacterium and related lactic acid bacteria and description of vagococcus salmoninarum sp. nov.the phylogenetic interrelationships of members of the genus carnobacterium and some atypical lactobacilli isolated from diseased salmonid fish were investigated by using reverse transcriptase sequencing of 16s rrna. the four species carnobacterium piscicola, carnobacterium divergens, carnobacterium gallinarum, and carnobacterium mobile exhibited a high degree of sequence similarity with each other (ca. 96 to 98%) and formed a phylogenetically coherent group that was quite distinct from all other ...19901697764
inhibition of listeria monocytogenes by carnobacterium spp. strains in a simulated cold smoked fish system stored at 4 degrees c.preservation of smoked salmon from bacterial spoilage, and especially from listeria monocytogenes by bacteriocin producers is a promising challenge. over a hundred lactic acid bacteria, isolated from commercial vacuum packaged cold smoked salmon, were screened for their antagonistic activity against l. innocua. twenty-two strains were able to produce bacteriocin-like proteinaceous substances. these strains were characterized physiologically and biochemically as carnobacterium strains. three diff ...199910357271
characteristics and genetic determinants of bacteriocin activities produced by carnobacterium piscicola cp5 isolated from cheese.carnobacterium piscicola cp5, isolated from a french mold-ripened soft cheese, produced a bacteriocin activity named carnocin cp5, which inhibited carnobacterium, enterococcus and listeria spp. strains, and among the lactobacillus spp. only lactobacillus delbrueckii spp. [24]. the activity was purified by ammonium sulfate precipitation, anion exchange, and hydrophobic interaction chromatography followed by reverse-phase high-performance liquid chromatography (rp-hplc). this latter step separated ...19979353214
chemical and genetic characterization of bacteriocins produced by carnobacterium piscicola lv17b.carnobacteriocins bm1 and b2 are thermostable class ii bacteriocins produced by carnobacterium piscicola lv17b. these bacteriocins were purified by a three-step procedure that included hydrophobic interaction, size exclusion, and reversed-phase high performance liquid chromatography. the purified peptides and fragments derived by enzymatic digestion were analyzed by edman degradation, amino acid analysis, and mass spectrometry. an oxidized form of carnobacteriocin bm1 (carnobacteriocin b1) was a ...19948163526
the contribution of bacteriocin to inhibition of listeria monocytogenes by carnobacterium piscicola strains in cold-smoked salmon systems.to study the importance of bacteriocin production for the antilisterial effect of a bacteriocinogenic carnobacterium piscicola strain a9b on growth of listeria monocytogenes in broth and cold-smoked salmon systems.200414678166
membrane-associated proteins encoded by the nisin gene cluster may function as a receptor for the lantibiotic carnocin ui49.carnocin ui49, a lantibiotic produced by carnobacterium piscicola shows bactericidal activity against many lactic acid bacteria. this paper describes the results of a study on the mode of action of carnocin ui49. it has previously been observed that nisin-producing lactococcus lactis subsp. lactis strains are at least 10-fold more sensitive to carnocin ui49 relative to other lactic acid bacteria. addition of carnocin ui49 to cells of l. lactis subsp. lactis nz9700 resulted in a dissipation of th ...19948081505
characterization of a locus from carnobacterium piscicola lv17b involved in bacteriocin production and immunity: evidence for global inducer-mediated transcriptional regulation.mutational, nucleotide sequence, and transcriptional analyses of a 10-kb fragment (carnobacteriocin locus) from the 61-kb plasmid of carnobacterium piscicola lv17b demonstrated the presence of two gene clusters (cbnxy and cbnskrtd) upstream of the previously sequenced carnobacteriocin b2 structural and immunity genes (cbnb2 and cbib2). deduced products of cbnk and cbnr have sequence similarity to proteins of agr-type two-component signal transduction systems, and those of cbnt and cbnd have sequ ...19979324267
isolation and characterization of carnobacterium, lactococcus, and enterococcus spp. from cooked, modified atmosphere packaged, refrigerated, poultry meat.the microbiota of commercially produced, cooked and modified atmosphere packaged poultry meat was followed during storage at 3.5 degrees c for up to 7 weeks. the dominant microbiota consisted of lactococcus raffinolactis (117 isolates), carnobacterium divergens (61 isolates), carnobacterium piscicola (11 isolates), lactococcus garvieae (four isolates), lactococcus lactis (one isolate) and enterococcus faecalis (three isolates). all isolates were screened for production of bacteriocins. only c. p ...200011139026
atypical genetic locus associated with constitutive production of enterocin b by enterococcus faecium bfe 900.a purified bacteriocin produced by enterococcus faecium bfe 900 isolated from black olives was shown by edman degradation and mass spectrometric analyses to be identical to enterocin b produced by e. faecium t136 from meat (p. casaus, t. nilsen, l. m. cintas, i. f. nes, p. e. hernández, and h. holo, microbiology 143:2287-2294, 1997). the structural gene was located on a 2.2-kb hindiii fragment and a 12.0-kb ecori chromosomal fragment. the genetic characteristics and production of entb by e. faec ...199910224016
growth control of listeria monocytogenes on cold-smoked salmon using a competitive lactic acid bacteria flora.a lactobacillus sake strain lke5 and four strains of carnobacterium piscicola were evaluated as biopreservation cultures to control the growth of listeria monocytogenes on vacuum-packed, cold-smoked salmon stored at 5 degrees c. all five strains were antilisterial as live cultures in an agar diffusion assay. cell-free supernatants of two strains of c. piscicola and l. sake lke5 were also antilisterial because of the production of bacteriocins. the presence of high cell numbers of strains of c. p ...199910419205
characterization of the genetic locus responsible for production and immunity of carnobacteriocin a: the immunity gene confers cross-protection to enterocin b.carnobacteriocin a (cbna) is a regulated bacteriocin produced by carnobacterium piscicola lv17a that is encoded on a 72 kb plasmid. a 10.0 kb fragment from this plasmid that contained information necessary for bacteriocin production and immunity was cloned and sequenced. genetic analysis showed the presence of the previously sequenced structural gene for cbna, as well as genes encoding proteins homologous to dedicated bacteriocin transport proteins and proteins of three-component signal transduc ...200010746765
explorative multivariate analyses of 16s rrna gene data from microbial communities in modified-atmosphere-packed salmon and coalfish.modified-atmosphere packaging (map) of foods in combination with low-temperature storage extends product shelf life by limiting microbial growth. we investigated the microbial biodiversity of map salmon and coalfish by using an explorative approach and analyzing both the total amounts of bacteria and the microbial group composition (both aerobic and anaerobic bacteria). real-time pcr analyses revealed a surprisingly large difference in the microbial loads for the different fish samples. the micr ...200415294842
effects of environmental factors on leucine catabolism by carnobacterium piscicola.the metabolites from leucine degradation are involved in dry fermented sausage aroma. the catabolism of leucine by a strain of carnobacterium piscicola was studied directly in the growth medium with 3h-labelled leucine to investigate the effect of five parameters: phase of growth, ph, oxygen, glucose and alpha-ketoisocaproic acid. resting cells (rc) were also incubated with 3h-labelled leucine. the radioactive metabolites from leucine catabolism were analysed by high performance liquid chromatog ...200312485743
description of a simple detection assay for in situ production of bacteriocin on meat.using a modification of the agar diffusion assay, in situ bacteriocin production on meat was analyzed using cooked meat medium (cmm) and sterile pork tissue (lean and fat) with carnobacterium piscicola lv17 as the producer and carnobacterium divergens lv13 as the indicator strains. contrary to what is observed in apt broth, bacteriocin production by c. piscicola lv17 occurred with growth at low inoculum levels (< or =10(4) cfu/cm2 or g of meat) on disks (10 cm2) of pork fat tissue (ph 6.58) and ...200111271779
growth inhibition of listeria monocytogenes by a nonbacteriocinogenic carnobacterium piscicola.this study elucidates the mechanisms by which a nonbacteriocinogenic carnobacterium piscicola inhibits growth of listeria monocytogenes.200515610430
purification and characterization of a novel class iia bacteriocin, piscicocin cs526, from surimi-associated carnobacterium piscicola cs526.the bacteriocin piscicocin cs526 was inactivated by proteolytic enzymes, was stable at 100 degrees c for 30 min, had a ph range of 2 to 8, and was active against enterococcus, listeria, pediococcus, and leuconostoc. the n-terminal sequence was ygngl, not the ygngv consensus motif common in class iia bacteriocins (alternate residues underlined). the molecular mass of piscicocin cs526, which had a bactericidal mode of action, was approximately 4,430 da.200515640235
inhibition of listeria monocytogenes by in situ produced and semipurified bacteriocins of carnobacterium spp. on vacuum-packed, refrigerated cold-smoked salmon.listeria monocytogenes inhibition by carnobacterium strains and crude bacteriocins on sterile and commercial vacuum-packed cold-smoked salmon stored at 4 degrees c and 8 degrees c was investigated. carnobacterium piscicola v1 was bactericidal against l. monocytogenes at the two temperatures, whereas carnobacterium divergens v41 presented a bacteriostatic effect. c. piscicola sf668 delayed l. monocytogenes growth at 8 degrees c and had a bacteriostatic effect at 4 degrees c. listeria growth was n ...199910606143
mutational analysis of the role of charged residues in target-cell binding, potency and specificity of the pediocin-like bacteriocin sakacin p.the significance of charged residues for the target-cell binding, potency and specificity of pediocin-like bacteriocins has been studied by site-directed mutagenesis of sakacin p. most of the charged residues are located in the n-terminal half, which is thought to mediate the initial binding of these bacteriocins to target cells through electrostatic interaction. all the mutated peptides in which the net positive charge was reduced by one (by replacing a charged residue with threonine) exhibited ...200212101290
simple method to identify bacteriocin induction peptides and to auto-induce bacteriocin production at low cell density.the production of some bacteriocins by lactic acid bacteria is regulated by induction peptides (ips) that are secreted by a dedicated secretion system. the ip gene cbax, for carnobacteriocin a production by carnobacterium piscicola lv17a, and a presumptive ip gene (orf6), associated with the genetic locus for enterocin b production in enterococcus faecium bfe 900, were fused to the signal peptide of the bacteriocin divergicin a from carnobacterium divergens lv13 to access the general secretory p ...200010802168
specific molecular detection of carnobacterium piscicola sf668 in cold smoked salmon.to establish a rapid and reliable multiplex pcr (mpcr)-based method allowing specific identification of carnobacterium piscicola sf668 during storage of cold smoked salmon (css).200515836740
shelf life and safety aspects of chilled cooked and peeled shrimps (pandalus borealis) in modified atmosphere packaging.to evaluate the growth of listeria monocytogenes and shelf life of cooked and peeled shrimps in modified atmosphere packaging (map).200515960666
numerical phenetic study of the genus carnobacterium.eighty-nine strains representing the genus carnobacterium, enterococcus durans, vagacoccus salmoninarum and atypical lactobacillus strains mt12 and mt13 were examined for 92 unit characters. computer analysis of the data resulted in the recovery of four major, five minor and thirteen single membered clusters. three cluster-groups contained seventy-four of the carnobacterium strains, enterococcus durans ncfb 596t and lactobacillus maltaromicus ncfb 2382t. cluster-group a was equated with carnobac ...200011016698
identification of lactic acid bacteria from spoiled, vacuum-packaged 'gravad' rainbow trout using ribotyping.a total of 296 lactic acid bacteria (lab) isolated from spoiled, vacuum-packaged 'gravad' rainbow trout stored at 3 and 8 degrees c were characterised and identified using a molecular approach. the isolates were initially grouped according to their hindiii restriction endonuclease profiles and further identified to species level using an rrna gene restriction pattern (ribotype) identification database. lactobacillus sakei, l. curvatus and carnobacterium piscicola were the three main species dete ...200211843406
enhancement of nisin production by lactococcus lactis in periodically re-alkalized cultures.synthesis of nisin as well as biomass production by lactococcus lactis subsp. lactis cect (colección española de cultivos tipo) 539 on both hydrolysed mussel-processing waste and whey medium were followed in three fixed volume fed-batch fermentations, with re-alkalization cycles. the two cultures on mussel-processing waste (mpw) were fed with a 240 g/l concentrated glucose and with a concentrated mpw (about 100 g of glucose/l). the culture on whey was fed with a mixture of concentrated whey (48 ...200312793859
innate immune responses in rainbow trout (oncorhynchus mykiss, walbaum) induced by probiotics.carnobacterium maltaromaticum b26 and carnobacterium divergens b33, which were isolated from the intestine of healthy rainbow trout (oncorhynchus mykiss, walbaum), were selected as being potentially useful as probiotics with effectiveness against aeromonas salmonicida and yersinia ruckeri. thus, rainbow trout administered with feed supplemented with b26 or b33 dosed at >10(7) cells g(-1) feed conferred protection against challenge with virulent cultures of the pathogens. moreover, both cultures ...200616631379
novel expression system for large-scale production and purification of recombinant class iia bacteriocins and its application to piscicolin 126.piscicolin 126 is a class iia bacteriocin isolated from carnobacterium piscicola jg126 that exhibits strong activity against listeria monocytogenes. the gene encoding mature piscicolin 126 (m-pisa) was cloned into an escherichia coli expression system and expressed as a thioredoxin-piscicolin 126 fusion protein that was purified by affinity chromatography. purified recombinant piscicolin 126 was obtained after cnbr cleavage of the fusion protein followed by reversed-phase chromatography. recombi ...200415184123
biochemical and phylogenetic analyses of a cold-active beta-galactosidase from the lactic acid bacterium carnobacterium piscicola ba.we are investigating glycosyl hydrolases from new psychrophilic isolates to examine the adaptations of enzymes to low temperatures. a beta-galactosidase from isolate ba, which we have classified as a strain of the lactic acid bacterium carnobacterium piscicola, was capable of hydrolyzing the chromogen 5-bromo-4-chloro-3-indolyl beta-d-galactopyranoside (x-gal) at 4 degrees c and possessed higher activity in crude cell lysates at 25 than at 37 degrees c. sequence analysis of a cloned dna fragment ...199910584002
sensory, chemical and bacteriological changes in vacuum-packed pressurised squid mantle (todaropsis eblanae) stored at 4 degrees c.sensory, chemical and bacteriological changes were studied in vacuum-packed squid mantles (todaropsis eblanae) that were pressurised at 150, 200, 300 and 400 megapascal (mpa) for 15 min at ambient temperature and stored at 4 degrees c. sensory analysis showed that the higher the pressurisation the longer the shelf-life. thus, the lot pressurised at 400 mpa was rejected after 28 days' storage compared with 7 days for the untreated lot. the chemical results generally corresponded with the sensory ...200211929163
carnocin kz213 produced by carnobacterium piscicola 213 is adsorbed onto cells during growth. its biosynthesis is regulated by temperature, ph and medium composition.carnocin kz213 is an antilisterial bacteriocin produced by carnobacterium piscicola 213. the effects of ph and temperature were studied during batch fermentation in mrs* medium (modified mrs without ammonium citrate or sodium acetate). the optimal ph for growth is between 6 and 7. the maximum bacteriocin productivity in the supernatant occurs at ph 7. operating at controlled ph increases the volumetric activity of the free bacteriocin by 8- to 16-fold, compared with uncontrolled ph. no bacterioc ...200414727147
influence of antimicrobial agents on the spoilage of a meat-based entomophage diet.the microbial decomposition of a meat-based entomophage diet presented in parafilm packets was investigated. considerable bacteria but not fungi were associated with components used to prepare the diet (i.e., hens' eggs, liver, and ground beef). at the initial sampling time, there were no differences among diet treatments in the size of bacterial or fungal populations. bacterial populations in diets not containing antibacterial agents rapidly increased and reached an asymptote by 24 h (approxima ...200415154441
effects of a bacteriocin-like inhibitory substance from carnobacterium piscicola against human and salmon isolates of listeria monocytogenes.the aim of this study was to characterize the antagonism of a bacteriocin-like inhibitory substance (blis) produced by carnobacterium piscicola l103 against listeria monocytogenes strains isolated from salmon and human samples. the inhibitory effect of the blis was evaluated in tryptic soy agar (tsa) during different growth phases of l. monocytogenes at 5 degrees c, using the well diffusion method. also, the type of inhibition, either bacteriostatic or bactericidal of the blis in tryptic soy bro ...200312781946
inhibition of listeria monocytogenes in cold-smoked salmon by carnobacterium piscicola cs526 isolated from frozen surimi.strain cs526 was isolated from frozen surimi and identified as a bacteriocin producer that had strong inhibitory activity against listeria monocytogenes. strain cs526 was identified as carnobacterium piscicola by partial 16s rdna sequence similarity. the ability of this bacteriocinogenic strain and nonbacteriocinogenic c. piscicola jcm5348 to inhibit the growth of l. monocytogenes was examined in culture broth incubated at 12 degrees c and cold-smoked salmon stored at 4, 12, and 20 degrees c. l. ...200312929829
characterization of two new glycosyl hydrolases from the lactic acid bacterium carnobacterium piscicola strain ba.three genes with homology to glycosyl hydrolases were detected on a dna fragment cloned from a psychrophilic lactic acid bacterium isolate, carnobacterium piscicola strain ba. a 2.2-kb region corresponding to an alpha-galactosidase gene, agaa, was followed by two genes in the same orientation, bgab, encoding a 2-kb beta-galactosidase, and bgac, encoding a structurally distinct 1.76-kb beta-galactosidase. this gene arrangement had not been observed in other lactic acid bacteria, including lactoco ...200111679331
inhibition of listeria monocytogenes by freeze-dried piscicocin cs526 fermentate in food.the effectiveness of freeze-dried powder, fermented with bacteriocin producing carnobacterium piscicola cs526, was evaluated for the inhibition of listeria monocytogenes in a food model.200717257251
limitations in the use of drosophila melanogaster as a model host for gram-positive bacterial infection.to examine sensitivities of various drosophila melanogaster strains towards human pathogenic and nonpathogenic gram-positive bacteria.200717257264
mode of action of piscicocin cs526 produced by carnobacterium piscicola cs526.piscicocin cs526 is a unique class iia bacteriocin produced by carnobacterium piscicola cs526. the mode of action against the sensitive strain listeria monocytogenes iid581 was evaluated.200515836484
isolation of carnobacterium piscicola from human pus--case report.carnobacterium piscicola was first described in 1984. these bacteria are often isolated from fish afflicted with bacterial infections. to date, there has been no reported isolation of this bacterium from human specimens. we report here the isolation of c. piscicola from the pus following traumatic amputation of the right hand in the wrist of a 35-year-old man. the traumatic amputation occurred with an industrial water sawmill. the identity of the human strain was determined biochemically, by 16s ...200212422528
immunomodulatory effects of nisin in turbot (scophthalmus maximus l.).in the present work, the effect of nisin on the non-specific immune response of turbot (scophthalmus maximus l.) leukocytes has been studied both in vitro and in vivo. the head kidney macrophage chemiluminescent (cl) response was significantly increased with intermediate doses of nisin (2.5 and 0.025 micro g ml(-1)) whilst the higher dose (25 micro g ml(-1)) significantly decreased the response after 24h incubation. when the incubation time was extended to 72 h, significant differences between d ...200312526879
inhibition of listeria innocua growth by antimicrobial-producing lactic acid cultures in vacuum-packed cold-smoked salmon.the biopreservative potential of three antimicrobial-producing lactic acid bacteria strains was evaluated on cold-smoked salmon. lactobacillus casei, lactobacillus plantarum and carnobacterium piscicola were added singly or in association to cold-smoked salmon, artificially contaminated with listeria innocua and stored under vacuum for 30 days at 4 degrees c. all the lactic cultures were able to inhibit listeria innocua growth, showing a bacteriostatic or bactericidal effect, without affecting n ...200616943070
nmr solution structure of the precursor for carnobacteriocin b2, an antimicrobial peptide from carnobacterium piscicola.type iia bacteriocins, which are isolated from lactic acid bacteria that are useful for food preservation, are potent antimicrobial peptides with considerable potential as therapeutic agents for gastrointestinal infections in mammals. they are ribosomally synthesized as precursors with an n-terminal leader, typically 18-24 amino acid residues in length, which is cleaved during export from the producing cell. we have chemically synthesized the full precursor of carnobacteriocin b2, precarnobacter ...200415096213
short communication: carnobacterium maltaromaticum: the only carnobacterium species in french ripened soft cheeses as revealed by polymerase chain reaction detection.the pcr technique using cb1-cb2r and species-specific primers was applied to various french soft flowered or washed rind cheeses to identify carnobacterium species. thirty cheeses made from cow's, ewe's, or goat's milk (raw or pasteurized), of which 20 were appellation d'origine contrôlée, were analyzed in the autumn and spring. the results revealed that, irrespective of the season, the carnobacterium genus was initially detected in 5 cheeses and was detected in 5 others after an enrichment peri ...200717297087
effect of bacterial interactions on the spoilage of cold-smoked salmon.cold-smoked salmon is a lightly preserved fish product in which a mixed microbial flora develops during storage and where the interactive behaviour of micro-organisms may contribute to their growth and spoilage activity. the aim of this study was to assess the effect of the bacterial interactions between the main species contaminating the cold-smoked salmon on bacterial growth, chemical and sensory changes, and spoilage. first, carnobacterium piscicola, photobacterium phosphoreum, lactobacillus ...200616949172
isolation and characterization of carnocyclin a, a novel circular bacteriocin produced by carnobacterium maltaromaticum ual307.carnobacterium maltaromaticum ual307, isolated from fresh pork, exhibits potent activity against a number of gram-positive organisms, including numerous listeria species. three bacteriocins were isolated from culture supernatant, and using matrix-assisted laser desorption ionization-time of flight mass spectrometry and edman sequencing, two of these bacteriocins were identified as piscicolin 126 and carnobacteriocin bm1, both of which have previously been described. the remaining bacteriocin, wi ...200818552180
carnobacterium divergens and carnobacterium maltaromaticum as spoilers or protective cultures in meat and seafood: phenotypic and genotypic characterization.carnobacterium, a genus of lactic acid bacteria, frequently dominate the microflora of chilled vacuum- or modified atmosphere-packed meat and seafood. in this study carnobacterium isolates were characterized by phenotypic and molecular methods in order to investigate the association of species and intra-species groups with distinct kinds of meat and seafood. of 120 test strains, 50 originated from meat (beef and pork products, including 44 strains isolated during this study and 6 strains obtaine ...200515830808
enterococcus species dominating in fresh modified-atmosphere-packaged, marinated broiler legs are overgrown by carnobacterium and lactobacillus species during storage at 6 degrees c.in order to show which of the initial lactic acid bacteria (lab) contaminants are also causing spoilage of a modified-atmosphere-packaged (map), marinated broiler leg product at 6 degrees c, lab were enumerated and identified on the 2nd and 17th days following manufacture. a total of 8 fresh and 13 spoiled packages were studied for lab levels. in addition, aerobic mesophilic bacteria and enterobacteriaceae were determined. the average cfu/g values in the 8 fresh packages were 1.3 x 10(3), 9.8 x ...200515582737
cytokine expression in leucocytes and gut cells of rainbow trout, oncorhynchus mykiss walbaum, induced by probiotics.understanding how the various host cells respond to probiotic bacteria in vitro may provide important insight into elaborate immune responses triggered by beneficial bacteria. the aim of this study was to investigate the detailed pattern of the mrna expression of cytokines (il-1beta, il-8, tnf-alpha and tgf-beta) in head kidney (hk) leucocytes and gut cells isolated from rainbow trout (oncorhynchus mykiss walbaum) after co-culturing with live probiotics. hk leucocytes and gut cells adjusted to 5 ...200617011045
functional characterization of a three-component regulatory system involved in quorum sensing-based regulation of peptide antibiotic production in carnobacterium maltaromaticum.quorum sensing is a form of cell-to-cell communication that allows bacteria to control a wide range of physiological processes in a population density-dependent manner. production of peptide antibiotics is one of the processes regulated by quorum sensing in several species of gram-positive bacteria, including strains of carnobacterium maltaromaticum. this bacterium and its peptide antibiotics are of interest due to their potential applications in food preservation. the molecular bases of the quo ...200617054797
selected nondigestible carbohydrates and prebiotics support the growth of probiotic fish bacteria mono-cultures in vitro.to search for nondigestible but fermentable (ndf) carbohydrates and prebiotics with a potency to promote the growth of selected bacteria in vitro.200919191975
mesophilic and psychrotrophic bacteria from meat and their spoilage potential in vitro and in beef.mesophilic and psychrotrophic populations from refrigerated meat were identified in this study, and the spoilage potential of microbial isolates in packaged beef was evaluated by analyzing the release of volatile organic compounds (voc) by gas chromatography-mass spectrometry (gc/ms). fifty mesophilic and twenty-nine psychrotrophic isolates were analyzed by random amplified polymorphic dna-pcr, and representative strains were identified by 16s rrna gene sequencing. carnobacterium maltaromaticum ...200919201980
whole-organism fingerprinting of the genus carnobacterium using fourier transform infrared spectroscopy (ft-ir).sixty seven strains of carnobacterium, atypical lactobacillus, enterococcus durans, lactobacillus maltaromicus and vagacoccus salmoninarum were examined by fourier transform infrared (ft-ir) spectroscopy. the effects of culture age and reproducibility over a six month period were also investigated. the results were analysed by multivariate statistics and compared with those from a previous numerical phenetic study, a pyrolysis mass spectrometry (pyms) study and with investigations which used dna ...200415046307
characterization of the bacterial spoilage flora in marinated pork products.to investigate the microbiota in marinated, vacuum-packed pork and to characterize isolated bacteria with regard to their spoilage potential.200919298510
effect of inoculation of carnobacterium divergens v41, a bio-preservative strain against listeria monocytogenes risk, on the microbiological, chemical and sensory quality of cold-smoked salmon.the aim of this study was to develop a bio-preservation strategy for cold-smoked salmon (css) by the use of lactic acid bacteria previously selected for their capability to inhibit the growth of listeria monocytogenes in the product. the spoiling potential of three carnobacterium strains (carnobacterium divergens v41, carnobacterium piscicola v1 and sf668) was tested in sterile css blocks inoculated by 10(4-5) cfu g(-)(1) and stored under vacuum for 9 days at 4 degrees c followed by 19 days at 8 ...200515979753
the circular bacteriocin, carnocyclin a, forms anion-selective channels in lipid bilayers.bacterial resistance to conventional antibiotics is a major challenge in controlling infectious diseases and has necessitated the development of novel approaches in antimicrobial therapy. one such approach is the use of antimicrobial peptides, such as the bacterially produced bacteriocins. carnocyclin a (ccla) is a 60-amino acid circular bacteriocin produced by carnobacterium maltaromaticum ual307 that exhibits potent activity against many gram-positive bacteria. lipid bilayer and single channel ...200919463781
a novel packaging method with a dissolving co(2) headspace combined with organic acids prolongs the shelf life of fresh salmon.the aim of this study was to evaluate a novel packaging method for fresh fish and to determine its effect on the bacterial growth in fresh salmon. fresh salmon was packed with a small amount of 100% co(2) (gas/product ratio 0.2/1.0 v/v) and a brine solution containing various combinations of citric acid (3% w/w, ph 5), acetic acid (1% w/w, ph 5) and cinnamaldehyde (200 microg/ml). total bacterial counts, counts of sulphur reducing bacteria, lactic acid bacteria and enterobacteriaceae as well as ...200919523706
characterization of probiotic carnobacteria isolated from rainbow trout (oncorhynchus mykiss) intestine.to characterize two probiotic carnobacterial isolates, carnobacterium maltaromaticum (b26) and c. divergens (b33), derived from rainbow trout (oncorhynchus mykiss) intestine.200819552776
antilisterial activity of a carnobacterium piscicola isolated from brazilian smoked fish (surubim [pseudoplatystoma sp.]) and its activity against a persistent strain of listeria monocytogenes isolated from surubim.data on the prevalence and growth of listeria monocytogenes in lightly preserved fish products from subtropical and tropical regions are very scarce. our research describes l. monocytogenes that was detected in 5% of the packages of cold-smoked surubim, a native brazilian freshwater fish that we analyzed, and shows that the strains isolated were of the same random amplified polymorphic dna subtype as the strains that were isolated from the same factory 4 years earlier. a bacteriocinogenic strain ...200516245709
role of broiler carcasses and processing plant air in contamination of modified-atmosphere-packaged broiler products with psychrotrophic lactic acid bacteria.some psychrotrophic lactic acid bacteria (lab) are specific meat spoilage organisms in modified-atmosphere-packaged (map), cold-stored meat products. to determine if incoming broilers or the production plant environment is a source of spoilage lab, a total of 86, 122, and 447 lab isolates from broiler carcasses, production plant air, and map broiler products, respectively, were characterized using a library of hindiii restriction fragment length polymorphism (rflp) patterns of the 16 and 23s rrn ...200717142357
technology-induced selection towards the spoilage microbiota of artisan-type cooked ham packed under modified atmosphere.the microbiota associated with a highly-perishable belgian artisan-type cooked ham was analyzed through plating and (gtg)(5)-fingerprinting of isolates throughout its processing chain. the raw tumbled meat was characterized by the presence of a versatile microbiota around 4.8 log(cfu g(-1)), consisting of lactic acid bacteria, staphylococci, brochothrix thermosphacta, gram-negative bacteria, and yeasts. pasteurisation of the ham logs reduced bacterial counts below 2 log(cfu g(-1)) and subsequent ...201019913696
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