| purification and properties of restriction endonuclease from deleya marina iam 14114, a marine bacterium (dmai). | a restriction endonuclease, designated as dmai, was purified from cell-free extracts of deleya marina iam 14114 by streptomycin treatment, ammonium sulfate fractionation and two steps of chromatographies on heparin-sepharose cl-6b and mono q (hr 5/5, fplc). the purified enzyme was homogeneous on sds-polyacrylamide gel disk electrophoresis and a ligation-recutting test. the relative molecular mass measurements of the purified enzyme gave 28,000 daltons by sds-polyacrylamide gel disk electrophores ... | 1990 | 1369311 |
| a restriction endonuclease (dpai) from deleya pacifica iam 14115, a marine bacterium, an isoschizomer of scai. | | 1991 | 1369358 |
| marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a new, extremely halotolerant, hydrocarbon-degrading marine bacterium. | on the basis of phenotypical characteristics and analysis of 16s rrna sequence, a new species belonging to a new genus is described, and the name marinobacter hydrocarbonoclasticus is proposed. this organism, isolated from mediterranean seawater near a petroleum refinery, is a gram-negative, aerobic, rod-shaped bacterium. it grows at nacl concentrations of 0.08 to 3.5 m and uses various hydrocarbons as the sole source of carbon and energy. its dna has a guanine-plus-cytosine content of 52.7 mol% ... | 1992 | 1382536 |
| characterization of a plasmid from moderately halophilic eubacteria. | a plasmid has been isolated for the first time from moderately halophilic eubacteria. halomonas elongata, halomonas halmophila, deleya halophila and vibrio costicola were found to harbour an 11.5 kbp plasmid (pmh1). the plasmid was isolated and characterized after transformation into escherichia coli jm101 cells. a restriction map was constructed, and unique restriction sites for ecori, ecorv and clai were detected. the occurrence of such a plasmid in the original halophilic strains was confirme ... | 1992 | 1527492 |
| polyamine distributions in the falvobacterium-cytophaga-sphingobacterium complex. | homospermidine was found as the major polyamine in one newly described species of flavobacterium (f. indologenes), in three species of sphingobacterium (s. mizutae, s. multivorium, and s. spiritivorum), and in 10 species of cytophaga (c. aquatilis, c. arvensicola, c. heparina, c. hutchinsonii, c. johnsonae, "c. keratolytica," c. lytica, c. marinoflava, c. uliginosa, and "c. xantha"). these bacteria also all contain putrescine and agmatine as minor components. flavobacterium indologenes and c. jo ... | 1991 | 1777864 |
| comparison of the adhesion properties of deleya marina and the exopolysaccharide-defective mutant strain dmr. | deleya marina 219 (atcc 25374) produces large quantities of an acidic exopolysaccharide and characteristically forms mucoid colonies and large aggregates of cells. the exopolysaccharide of wild-type d. marina cells appears to occur as both film and fibrils in electron micrographs. the organization of exopolymeric material was indicative of structural heterogeneity. a spontaneous rough-colony mutant defective in exopolysaccharide, d. marina dmr, has been isolated. the absence of exopolymer corres ... | 1991 | 1781675 |
| effect of external salinity changes on cellular composition of some ions and amino acids in deleya halophila. | changes in total cellular composition of some ions and amino acids of the moderate halophile deleya halophila were studied in response to external salinity changes. among the 14 amino acids investigated, the cellular glycine and aspartic acid content increased with increasing salinity. d. halophila also accumulated na+ at the highest external salt concentrations. | 1991 | 2068378 |
| growth rate and some physiological features of deleya halophila ccm 3662 at different salt concentrations. | the growth rate of the type strain of the new, moderately halophilic species deleya halophila (deleya halophila ccm 3662) at different salt concentrations was determined and its optimal marine salt concentration for growth was established. oxygen uptake and some cellular chemical composition characteristics of cells grown at their optimal salt concentration (7.5% w/v) and at a higher concentration (15% w/v) were compared, showing that both protein and carbohydrate intracellular contents decrease ... | 1987 | 3606873 |
| phylogenetic inferences and taxonomic consequences of 16s ribosomal dna sequence comparison of chromohalobacter marismortui, volcaniella eurihalina, and deleya salina and reclassification of v. eurihalina as halomonas eurihalina comb. nov. | the phylogenetic positions of the moderately halophilic bacteria chromohalobacter marismortui, volcaniella eurihalina, and deleya salina were determined by pcr amplification of rrna genes and direct sequencing. the resulting data were compared with data for other bacteria obtained from 16s rrna sequence databases. c. marismortui, v. eurihalina, and d. salina clustered phylogenetically within the gamma subclass of the proteobacteria and are closely related to other species on the halomonas-deleya ... | 1995 | 7547290 |
| convergent evolution of amino acid usage in archaebacterial and eubacterial lineages adapted to high salt. | chemical composition and physical properties of the total protein of haloferax mediterranei, a halophilic archaebacterium requiring high salt concentration for growth, of halomonas elongata, a halotolerant eubacterium able to grow at any concentration of salt, and of escherichia coli b, a eubacterium related to h. elongata, unable to grow at high salt concentration, were compared using robust standard biochemical methods. the distribution of amino acid abundancies in the bulk protein from h. elo ... | 1995 | 7652205 |
| transposon mutagenesis in halophilic eubacteria: conjugal transfer and insertion of transposon tn5 and tn1732 in halomonas elongata. | molecular genetic studies of halophilic eubacteria have been limited by the lack of a suitable method for mutagenesis. to overcome this, we established a transposon mutagenesis procedure for the ectoine-producing, halophilic bacterium halomonas elongata. we used suicide plasmids psup101 and psup102-gm to introduce the transposons tn5 and tn1732 respectively into h. elongata via escherichia coli sm10 mediated conjugation. our finding that h. elongata is sensitive to aminoglycoside antibiotics at ... | 1995 | 7781978 |
| isolation of cryptic plasmids from moderately halophilic eubacteria of the genus halomonas. characterization of a small plasmid from h. elongata and its use for shuttle vector construction. | three cryptic plasmids have been isolated from moderately halophilic eubacteria belonging to three species of the genus halomonas. these three plasmids were designated phe1 (4.2 kb, isolated from h. elongata atcc 33174), phi1 (48 kb, isolated from "h. israelensis" atcc 43985), and phs1 (ca. 70 kb, isolated from h. subglaciescola uqm 2927). because of its small size, the plasmid phe1 was selected for further characterization and construction of a shuttle vector for halomonas strains. phe1 was clo ... | 1995 | 7891654 |
| proteolytic and lipolytic activities of micrococcus roseus (65), halomonas elongata (16) and vibrio sp. (168) isolated from danish bacon curing brines. | viable cells, cell free extracts and extracellular concentrates of micrococcus roseus (65), halomonas elongata (16) and vibrio sp. (168) isolated from danish bacon curing brines were examined for lipase, esterase, proteinase and aminopeptidase activities on natural and synthetic substrates. micrococcus roseus (65) produced one intracellular esterase with affinity for short chain esters, and two intracellular aminopeptidases with affinity for nonpolar amino acids and l-arginine, respectively. one ... | 1994 | 8074966 |
| phylogenetic relationships of marine bacteria, mainly members of the family vibrionaceae, determined on the basis of 16s rrna sequences. | the phylogenetic relationships of 50 reference strains, mostly marine bacteria which require na+ for growth, were determined on the basis of 600 16s rrna nucleotides by using reverse transcriptase sequencing. strains belonging to 10 genera were included (four genera of the family vibrionaceae, the genus aeromonas of the family aeromonadaceae, and the genera alteromonas, marinomonas, shewanella, pseudomonas, and deleya). the sequences were aligned, the similarity values and evolutionary distance ... | 1993 | 8427811 |
| development of a gene reporter system in moderately halophilic bacteria by employing the ice nucleation gene of pseudomonas syringae. | the expression of the ice nucleation gene inaz of pseudomonas syringae in several moderate halophiles was investigated to establish its utility as a reporter for promoter activity and gene expression studies in these biotechnologically and environmentally important bacteria. a promoterless version of inaz was introduced in two different restriction sites and at both orientations in a recombinant plasmid able to replicate in moderate halophiles and, in particular, within the sequence of its phe1 ... | 1995 | 8526492 |
| identification of bacteria in a biodegraded wall painting by denaturing gradient gel electrophoresis of pcr-amplified gene fragments coding for 16s rrna. | medieval wall paintings are often affected by biodecay. an inventory of the existing microorganisms associated with the damage to the paintings is not yet an integral part of the restoration process. this stems from the lack of effective means for such a stocktaking. nevertheless, fungi and bacteria cause severe damage through mechanical processes from growth into the painting and its grounding and through their metabolism. detailed information on the bacterial colonization of ancient wall paint ... | 1996 | 8787403 |
| construction of novel shuttle vectors for use between moderately halophilic bacteria and escherichia coli. | shuttle vectors useful for the genetic manipulation of several moderately halophilic bacteria have been constructed. these vectors are based on the minimal replicon of pcm1, a cryptic plasmid from chromohalobacter marismortui, combined with the useful properties of puc18 plasmid (i.e., small size, high copy number, multiple cloning sites, lacz fragment), as well as with the trimethoprim resistance gene as a selection marker for moderate halophiles. these vectors can be efficiently transferred by ... | 1995 | 8825368 |
| osmoprotectants in halomonas elongata: high-affinity betaine transport system and choline-betaine pathway. | the osmoregulatory pathways of the moderately halophilic bacterium halomonas elongata dsm 3043 have been investigated. this strain grew optimally at 1.5 to 2 m nacl in m63 glucose-defined medium. it required at least 0.5 m nacl for growth, which is a higher concentration than that exhibited by the h. elongata type strain atcc 33173. externally provided betaine, choline, or choline-o-sulfate (but not proline, ectoine, or proline betaine) enhanced the growth of h. elongata on 3 m nacl-glucose-m63 ... | 1996 | 8955405 |
| salt-sensitive and auxotrophic mutants of halomonas elongata and h. meridiana by use of hydroxylamine mutagenesis | the killing action and induced mutagenicity inhydroxylamine (ha)-treated cells of two moderately halophilic species of thegenus halomonas, h. elongata and h. meridiana, wereinvestigated. a high sensitivity of h. elongata and especially ofh. meridiana to ha was found. the efficiency of the mutagenicityobtained with the ha treatment was tested at different salinities. optimalexperimental conditions for ha mutagenesis of these two moderate halophileswere determined. a clear, efficient mutagenicity ... | 1997 | 9003584 |
| microbulbifer hydrolyticus gen. nov., sp. nov., and marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community. | two numerically important bacteria in marine pulp mill effluent enrichment cultures were isolated. these organisms were gram-negative, rod-shaped, strictly aerobic bacteria. isolate ire-31t (t = type strain) produced hydrolytic enzymes for the breakdown of cellulose, xylan, chitin, gelatin, and tween 80. it also utilized a variety of monosaccharides, disaccharides, amino acids, and volatile fatty acids for growth. isolate kw-40t did not utilize natural polymers, but it could grow on a variety of ... | 1997 | 9103623 |
| diversity and association of psychrophilic bacteria in antarctic sea ice. | the bacterial populations associated with sea ice sampled from antarctic coastal areas were investigated by use of a phenotypic approach and a phylogenetic approach based on genes encoding 16s rrna (16s rdna). the diversity of bacteria associated with sea ice was also compared with the bacterial diversity of seawater underlying sea ice. psychrophilic (optimal growth temperature, < or = 15 degrees c; no growth occurring at 20 degrees c) bacterial diversity was found to be significantly enriched i ... | 1997 | 9251193 |
| identification of a promoter region on the halomonas elongata cryptic plasmid phe1 employing the inaz reporter gene of pseudomonas syringae. | a native promoter located on the cryptic plasmid phe1 from the moderate halophile halomonas elongata was identified employing a promoterless ice nucleation gene inaz of pseudomonas syringae by direct subcloning and assaying for ice nucleation activity. the presence of the promoter was verified by inserting the corresponding intact or deleted phe1 fragment in the promoter analysis vector pkk232-8 upstream of the promoterless cat or inaz gene. only constructs carrying the intact phe1 fragment gave ... | 1997 | 9297819 |
| construction and characterization of an nacl-sensitive mutant of halomonas elongata impaired in ectoine biosynthesis. | using transposon mutagenesis we generated a salt-sensitive mutant of the halophilic eubacterium halomonas elongata impaired in the biosynthesis of the compatible solute ectoine. hplc determinations of the cytoplasmic solute content showed the accumulation of a biosynthetic precursor of ectoine, l-2,4-diaminobutyric acid. ectoine and hydroxyectoine were not detectable. this mutant failed to grow in minimal medium with nacl concentrations exceeding 4%. however, when supplemented with organic osmol ... | 1998 | 9570121 |
| chemically emulsified crude oil as substrate for bacterial oxidation: differences in species response. | four bacterial species were tested for their abilities to oxidize alkanes in crude oil in water emulsions. the emulsions were prepared by nonionic sorbitan ester and polyoxyethylene ether surfactants. the oxidation rates were measured as initial attack on the emulsions by resting cells pregrown in crude oil media. the bacteria responded differently and both positive and negative effects of surfactant amendment were observed. the same surfactant affected various bacteria differently and the respo ... | 1998 | 9575027 |
| carnimonas nigrificans gen. nov., sp. nov., a bacterial causative agent for black spot formation on cured meat products. | nine different strains, ctcbs1t or ctcbs9, were identified to be the causative agents of black spots on the surface of raw cured meat products. the formation of black spots under aerobic conditions is reproducible upon reinoculation of meat products with any of these strains, indicating that they are the causative agent. the strains were gram-negative, catalase-positive and obligately aerobic rods. the g+c content of dna of strain ctcbs1t is 56.0 +/- 0.3 mol%. the content of non-polar main fatty ... | 1998 | 9734022 |
| synthesis of glycine betaine from exogenous choline in the moderately halophilic bacterium halomonas elongata | the role of choline in osmoprotection in the moderate halophile halomonas elongata has been examined. transport and conversion of choline to betaine began immediately after addition of choline to the growth medium. intracellular accumulation of betaine synthesized from choline was salt dependent up to 2.5 m nacl. oxidation of choline was enhanced at 2.0 m nacl in the presence or absence of externally provided betaine. this indicates that the nacl concentration in the growth medium has major effe ... | 1998 | 9758852 |
| characterization of biosynthetic enzymes for ectoine as a compatible solute in a moderately halophilic eubacterium, halomonas elongata. | 1,4,5,6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid (ectoine) is an excellent osmoprotectant. the biosynthetic pathway of ectoine from aspartic beta-semialdehyde (asa), in halomonas elongata, was elucidated by purification and characterization of each enzyme involved. 2,4-diaminobutyrate (daba) aminotransferase catalyzed reversively the first step of the pathway, conversion of asa to daba by transamination with l-glutamate. this enzyme required pyridoxal 5'-phosphate and potassium ions for i ... | 1999 | 9864317 |
| surface-grafted, environmentally sensitive polymers for biofilm release. | controlling bacterial biofouling is desirable for almost every human enterprise in which solid surfaces are introduced into nonsterile aqueous environments. one approach that is used to decrease contamination of manufactured devices by microorganisms is using materials that easily slough off accumulated material (i.e., fouling release surfaces). the compounds currently used for this purpose rely on low surface energy to inhibit strong attachment of organisms. in this study, we examined the possi ... | 1999 | 10103257 |
| novel diglycosyldiacylglycerol from the gram-negative bacterium deleya marina. | a glycosyldiacylglycerol was isolated from the marine bacterium deleya marina (atcc 25374). the structure was determined, mainly by spectral data, to be 1, 2-diacyl-3-o-[alpha-2-amino-2-deoxy-glucopyranose-(1-->4)-o-alpha-idu ronopyranuronic acid]-glycerol. this is, to our knowledge, the first isolation of diglycosyldiacylglycerol containing both iduronopyranuronic acid and 2-amino-2-deoxy-glucopyranose from gram-negative bacteria. | 1998 | 9714788 |
| role of ngamma-acetyldiaminobutyrate as an enzyme stabilizer and an intermediate in the biosynthesis of hydroxyectoine. | strain chr63 is a salt-sensitive mutant of the moderately halophilic wild-type strain halomonas elongata dsm 3043 that is affected in the ectoine synthase gene (ectc). this strain accumulates large amounts of ngamma-acetyldiaminobutyrate (nada), the precursor of ectoine (d. cánovas, c. vargas, f. iglesias-guerra, l. n. csonka, d. rhodes, a. ventosa, and j. j. nieto, j. biol. chem. 272:25794-25801, 1997). hydroxyectoine, ectoine, and glucosylglycerate were also identified by nuclear magnetic reso ... | 1999 | 10473374 |
| the use of fatty acid methyl ester analysis (fame) for the identification of heterotrophic bacteria present on three mural paintings showing severe damage by microorganisms. | mural paintings in carmona (spain), herberstein (austria) and greene (germany), showing visible deterioration by microorganisms, were sampled to investigate the biodiversity of the heterotrophic bacteria present. four hundred twenty-eight bacterial strains were isolated from which 385 were characterized by fatty acid methyl ester analysis (fame). the isolates were grouped into 41 clusters on the basis of their fame profiles, 20 isolates remained ungrouped. the majority (94%) of the isolates comp ... | 1999 | 10564789 |
| production and biochemical characterization of an alpha-amylase from the moderate halophile halomonas meridiana. | extracellular amylase production by the moderate halophile halomonas meridiana was optimized and the enzyme was characterized biochemically. the highest amylase production was achieved by growing h. meridiana cultures in media with 5% salts and starch, in the absence of glucose until the end of the exponential phase. the amylase exhibited maximal activity at ph 7.0, being relatively stable in alkaline conditions. optimal temperature and salinity for activity were 37 degrees c and 10% nacl, respe ... | 2000 | 10650204 |
| culture-dependent and culture-independent characterization of microbial assemblages associated with high-temperature petroleum reservoirs. | recent investigations of oil reservoirs in a variety of locales have indicated that these habitats may harbor active thermophilic prokaryotic assemblages. in this study, we used both molecular and culture-based methods to characterize prokaryotic consortia associated with high-temperature, sulfur-rich oil reservoirs in california. enrichment cultures designed for anaerobic thermophiles, both autotrophic and heterotrophic, were successful at temperatures ranging from 60 to 90 degrees c. heterotro ... | 2000 | 10653739 |
| self-assembling amphiphilic siderophores from marine bacteria. | most aerobic bacteria secrete siderophores to facilitate iron acquisition. two families of siderophores were isolated from strains belonging to two different genera of marine bacteria. the aquachelins, from halomonas aquamarina strain ds40m3, and the marinobactins, from marinobacter sp. strains ds40m6 and ds40m8, each contain a unique peptidic head group that coordinates iron(iii) and an appendage of one of a series of fatty acid moieties. these siderophores have low critical micelle concentrati ... | 2000 | 10678827 |
| single-step purification of a recombinant thermostable alpha-amylase after solubilization of the enzyme from insoluble aggregates. | the expression of the gene encoding a thermostable alpha-amylase (ec 3.2.1.1) (optimal activity at 100 degrees c) from the hyperthermophilic archaeon pyrococcus woesei in the mesophilic hosts escherichia coli and halomonas elongata resulted in the formation of insoluble aggregates. more than 85% of the recombinant enzyme was present within the cells as insoluble but catalytically active aggregates. the recombinant alpha-amylase was purified to homogeneity in a single step by hydrophobic interact ... | 2000 | 10681062 |
| genes for the synthesis of the osmoprotectant glycine betaine from choline in the moderately halophilic bacterium halomonas elongata dsm 3043, usa. | the genes involved in the oxidative pathway of choline to glycine betaine in the moderate halophile halomonas elongata dsm 3043 were isolated by functional complementation of an escherichia coli strain defective in glycine betaine synthesis. the cloned region was able to mediate the oxidation of choline to glycine betaine in e. coli, but not the transport of choline, indicating that the gene(s) involved in choline transport are not clustered with the glycine betaine synthesis genes. nucleotide s ... | 2000 | 10708384 |
| nuclear magnetic resonance studies of cesium-133 in the halophilic halotolerant bacterium ba1. chemical shift and transport studies. | ba1 bacteria (halomonas israelensis) were grown on different salt concentrations 0.2-4 m. when the cells were transferred to a medium containing 25 mm cscl without potassium there was an uptake of cesium by the cells. the intracellular cesium signal was shifted from the cesium signal in the medium without the use of a shift reagent. the shift was depended on the salt concentration in the growth medium. the intracellular cesium shift showed a much smaller dependence on the concentration of salts ... | 1998 | 9608592 |
| isolation and characterization of salt-sensitive mutants of the moderate halophile halomonas elongata and cloning of the ectoine synthesis genes. | the moderate halophile halomonas elongata deustche sammlung für mikroorganismen 3043 accumulated ectoine, hydroxyectoine, glutamate, and glutamine in response to osmotic stress (3 m nacl). two tn1732-induced mutants, chr62 and chr63, that were severely affected in their salt tolerance were isolated. mutant chr62 could not grow above 0.75 m nacl, and chr63 did not grow above 1.5 m nacl. these mutants did not synthesize ectoine but accumulated ectoine precursors, as shown by 13c nmr and mass spect ... | 1997 | 9325308 |
| osmotically induced response in representatives of halophilic prokaryotes: the bacterium halomonas elongata and the archaeon haloferax volcanii. | haloferax volcanii and halomonas elongata have been selected as representatives of halophilic archaea and bacteria, respectively, to analyze the responses to various osmolarities at the protein synthesis level. we have identified a set of high-salt-related proteins (39, 24, 20, and 15.5 kda in h. elongata; 70, 68, 48, and 16 kda in h. volcanii) whose synthesis rates increased with increasing salinities. a different set of proteins (60, 42, 15, and 6 kda for h. elongata; 63, 44, 34, 18, 17, and 6 ... | 1997 | 9287003 |
| n-acyl amino acid biosynthesis in marine bacterium, deleya marina. | we reported previously that the marine bacterium, deleya marina (atcc 25374), produced n-acyl leucine and isoleucine, in which nonhydroxy fatty acid was linked to alpha-amino group of amino acid. further analysis of bacterium lipids revealed the additional production of n-acyl ornithine. the n-acyl ornithine had a 3-hydroxy fatty acid linked by an amide bond to a-amino group of ornithine and a nonhydroxy fatty acid esterified to the hydroxy group of the 3-hydroxy fatty acid. n-acyl ornithine was ... | 1997 | 9271247 |
| isolation from coastal sea water and characterization of bacterial strains involved in non-ionic surfactant degradation. | a bacterial community degrading branched alkylphenol ethoxylate (ape) was selected from coastal sea water intermittently polluted by urban sewage. this community degraded more than 99% of a standard surfactant, triton x 100, but i.r. analysis of the remaining compound showed the accumulation of ape2 (alkylphenol with a two units length ethoxylated chain) which seemed very recalcitrant to further biodegradation. twenty-five strains were isolated from this community, essentially gram negative and ... | 1996 | 9144968 |
| phylogenetic analysis of psychrophilic bacteria isolated from the japan trench, including a description of the deep-sea species psychrobacter pacificensis sp. nov. | phylogenetic positions of psychrophilic bacteria isolated from the japan trench were determined by sequencing analysis of pcr-amplified bacterial small subunit (16s) rrna genes. between surface and deep-sea psychrophiles, distinct positions clearly differed within the gamma-proteobacteria. in phylogenetic analysis using neighbour-joining, maximum-parsimony and maximum-likelihood, strains from surface seawater were inferred to be located in the halomonas aquamarina-meridiana clade within the fami ... | 2000 | 10758895 |
| ectoine, the compatible solute of halomonas elongata, confers hyperosmotic tolerance in cultured tobacco cells. | 1,4,5,6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid (ectoine) functions as a compatible osmolyte in the moderate halophile halomonas elongata out30018. ectoine is biosynthesized by three successive enzyme reactions from aspartic beta-semialdehyde. the genes encoding the enzymes involved in the biosynthesis, ecta, ectb, and ectc, encoding l-2,4-diaminobutyric acid acetyltransferase, l-2, 4-diaminobutyric acid transaminase, and l-ectoine synthase, respectively, have been previously cloned. to ... | 2000 | 10759521 |
| the alpha-amylase gene amyh of the moderate halophile halomonas meridiana: cloning and molecular characterization. | two types of tn1732-induced mutants defective in extracellular amylase activity were isolated from the moderate halophile halomonas meridiana dsm 5425. type i mutants displayed amylase activity in the periplasm, and were unable to use any of the carbon sources tested, including starch and its hydrolysis product maltose. the type ii mutant was affected in the gene responsible for the synthesis of the extracellular alpha-amylase. this gene (amyh) was isolated by functional complementation of mutan ... | 2000 | 10784044 |
| importance of passive diffusion in the uptake of polychlorinated biphenyls by phagotrophic protozoa. | unicellular protozoan grazers represent a size class of organisms where a transition in the mechanism of chlorobiphenyl (cb) introduction, from diffusion through surface membranes to ingestion of contaminated prey, could occur. this study compares the relative importance of these two processes in the overall uptake of polychlorinated biphenyls by protists. uptake rates and steady-state concentrations were compared in laboratory cultures of grazing and nongrazing protozoa. these experiments were ... | 2000 | 10788371 |
| genetic organization of the mobilization region of the plasmid phe1 from halomonas elongata. | the mobilization (mob) region of the non-self transmissible 4.2-kb plasmid phe1 from the moderately halophilic bacterium halomonas elongata atcc 33174 has been identified and characterized. analysis of the sequence revealed the presence of four open reading frames (mobcabd) which show a complex organization with two of them (mobb and mobd) entirely overlapped by a third (moba). the deduced proteins appeared to have a high degree of homology to mob proteins of coie1 and closely related plasmids. ... | 1999 | 10794139 |
| attachment of bacteria to model solid surfaces: oligo(ethylene glycol) surfaces inhibit bacterial attachment. | bacterial cell attachment to the surfaces of self-assembled monolayers formed by the adsorption of omega-substituted alkanethiols on transparent gold films has been studied under defined bacterial culture and flow conditions. phase contrast microscopy was used to quantify the attachment of two organisms, one of medical (staphylococcus epidermidis) and one of marine (deleya marina) importance. self-assembled monolayers terminated with hexa(ethylene glycol), methyl, carboxylic acid and fluorocarbo ... | 1996 | 8759791 |
| molecular analysis of maleate cis-trans isomerase from thermophilic bacteria. | several strains of thermophilic bacteria containing maleate cis-trans isomerase were isolated from soil samples and identified as bacillus stearothermophilus, bacillus circulans, bacillus brevis, and deleya halophila. the maleate cis-trans isomerase was purified and characterized from one of the isolated strains, b. stearothermophilus mi-102. the purified enzyme of strain mi-102 showed higher thermal stability than the enzyme of a mesophile, alcaligenes faecalis ifo13111. the seven maleate cis-t ... | 2000 | 10803955 |
| enzyme electrochemical preparation of a 3-keto derivative of 1,5-anhydro-d-glucitol using glucose-3-dehydrogenase. | a novel enzymatic organic synthesis was reported, utilizing glucose-3-dehydrogenase (g3dh) and its regeneration via electrochemical methods. we combined the water-soluble g3dh prepared from a marine bacterium, halomonas sp. alpha-15, and electron mediator with the electrode system in order to regenerate the enzyme. using this system, the conversion of 1,5-anhydro-d-glucitol (1,5ag), a diabetes marker in human blood, was investigated. the final yield of the product, 3-keto anhydroglucitol (3-keto ... | 2000 | 10849848 |
| the role of the outer membrane in formaldehyde tolerance in escherichia coli vu3695 and halomonas sp. mac. | to investigate the mechanism of formaldehyde tolerance in gram-negative bacteria, two formaldehyde-tolerant strains, escherichia coli vu3695 and halomonas sp. mac (dsm 7328), and formaldehyde-sensitive revertants obtained by ethidium bromide or novobiocin treatment were studied. the presence of high levels of formaldehyde dehydrogenase activity alone proved insufficient to confer tolerance to high formaldehyde concentrations, as shown by high activity displayed by formaldehyde-sensitive revertan ... | 1996 | 8704965 |
| process of carbonate precipitation by deleya halophila | scanning electron microscopy and x-ray dispersive energy microanalysis were used to investigate the formation of carbonate crystals by deleya halophila. the formation of calcium carbonate crystals (polymorphous aragonite) by d. halophila is a sequential process that commences with a nucleus formed by the aggregation of a few calcified bacterial cells and the subsequent accumulation of more calcified cells and carbonate, which acts to weld the bacteria together. the process leads to the formation ... | 1996 | 8661675 |
| description of two new species of halomonas: halomonas israelensis sp.nov. and halomonas canadensis sp.nov. | six well-known strains of halotolerant bacteria, including two strains previously identified only as nrcc 41227 and ba1, have been compared using 125 phenotypic characters and dna-dna hybridization. although these strains represent some of the most heavily studied salt-tolerant bacteria, they have never been taxonomically compared. the data presented show that these bacteria form a relatively homogeneous group related at the genus level. the taxonomic comparison showed that these six organisms r ... | 1995 | 8542554 |
| influence of salt concentration on the susceptibility of moderately halophilic bacteria to antimicrobials and its potential use for genetic transfer studies. | the influence of salinity on the susceptibility of 13 moderately halophilic collection strains belonging to the genera chromohalobacter, deleya, halomonas, vibrio, and volcaniella to 10 common antimicrobials has been studied. three different patterns of tolerance were found when salinity was varied from 10 to 1% (wt/vol) total salts in the testing media. the first one included the responses to ampicillin and rifampicin, where only minimal effects on the susceptibility were found. all moderate ha ... | 1995 | 8528008 |
| release of cell-free ice nuclei from halomonas elongata expressing the ice nucleation gene inaz of pseudomonas syringae. | release of ice nuclei in the growth medium of recombinant halomonas elongata cells expressing the inaz gene of pseudomonas syringae was studied in an attempt to produce cell-free active ice nuclei for biotechnological applications. cell-free ice nuclei were not retained by cellulose acetate filters of 0.2 microm pore size. highest activity of cell-free ice nuclei was obtained when cells were grown in low salinity (0.5-5% nacl, w/v). freezing temperature threshold, estimated to be below -7 degree ... | 2000 | 11119152 |
| phylogenetic relationships between some members of the genera deleya, halomonas, and halovibrio. | the genera halomonas and deleya, which constitute the family halomonadaceae, are difficult to differentiate on the basis of phenotypic and chemotaxonomic attributes. dna-rrna hybridization studies have indicated that some halomonas spp. have the same level of relationship to the type species of the genus deleya as some deleya spp. a phylogenetic analysis of the 16s rrna sequences of seven members of the halomonadaceae indicated that the members of the genera halomonas and deleya do not form sepa ... | 1993 | 8240950 |
| airborne environmental endotoxin: a cross-validation of sampling and analysis techniques. | a standard method for measurement of airborne environmental endotoxin was developed and field tested in a fiberglass insulation-manufacturing facility. this method involved sampling with a capillary-pore membrane filter, extraction in buffer using a sonication bath, and analysis by the kinetic-limulus assay with resistant-parallel-line estimation (klare). cross-validation of the extraction and assay method was performed by comparison with methanolysis of samples followed by 3-hydroxy fatty acid ... | 1994 | 8161191 |
| enzymatic synthesis of a novel trehalose derivative, 3,3'-diketotrehalose, and its potential application as the trehalase enzyme inhibitor. | we reported the preparation of a novel trehalose derivative based on enzymatic oxidation of trehalose by water-soluble glucose-3-dehydrogenase (g3dh) from marine bacterium halomonas sp. alpha-15 cells. the product of g3dh enzymatic conversion was 3,3'-diketotrehalose (3,3'dkt), a novel trehalose derivative of which both third hydroxy groups of glucopyranosides were oxidized. 3,3'dkt was revealed to show an inhibitory effect toward pig-kidney and bombyx mori trehalases. the ic(50) values of 3,3'd ... | 2001 | 11231010 |
| cloning and expression of glucose 3-dehydrogenase from halomonas sp. alpha-15 in escherichia coli. | the gene encoding glucose 3-dehydrogenase (g3dh) from halomonas sp. alpha-15 was cloned and expressed in escherichia coli. an open reading frame of 1686 nucleotides was shown to encode g3dh. the flavine adenine dinucleotide binding motif was found in the n-terminal region of g3dh. the deduced primary structure of g3dh showed about 30% identity to sorbitol dehydrogenase from gluconobacter oxydans and 2-keto-d-gluconate dehydrogenases from erwinia herbicola and pantoea citrea. the folding predicti ... | 2001 | 11263965 |
| characterization of facultative oligotrophic bacteria from polar seas by analysis of their fatty acids and 16s rdna sequences. | one hundred and seventy three bacterial strains, isolated previously after enrichment under oligotrophic, psychrophylic conditions from arctic (98 strains) and antarctic seawater (75 strains), were characterized by gas-liquid chromatographic analysis of their fatty acid compositions. by numerical analysis, 8 clusters, containing 2 to 59 strains, could be delineated, and 8 strains formed separate branches. five clusters contained strains from both poles, two minor clusters were confined to arctic ... | 2001 | 11403404 |
| effects of nacl on the uptake of alpha-[14c]aminoisobutyric acid by the halotolerant bacterium halomonas elongata. | the alpha-aminoisobutyric acid (aib) transport system of the halotolerant bacterium, halomonas elongata, was examined. cells were grown in l-alanine defined medium with 0.05, 0.375, 1.37, 2.5, or 3.4 m nacl. each group of cells was resuspended in buffered salts with different nacl concentrations (0.05, 0.375, 1.37, 2.5, and 3.4 m) and the uptake of alpha-[14c]aib was measured. optimum aib uptake occurred in the 0.375 m nacl solution for the lower salt grown cells and the 1.37 m nacl solution for ... | 1983 | 6661704 |
| cell wall and phospholipid composition and their contribution to the salt tolerance of halomonas elongata. | the salt-tolerant bacterium halomonas elongata makes a variety of physiological adaptations in response to increases in the salt concentration of its growth medium. the cell walls become more compact and internally coherent. the overall lipid pattern shows an increased amount of negatively charged lipids. in addition, the peptidoglycan composition of h. elongata, although not changing in response to increased nacl, contains the hydrophobic amino acid leucine which is unique among bacterial speci ... | 1984 | 6501232 |
| distribution and types of bacteria isolated from an inland saltern. | the physico-chemical characteristics and bacterial populations of an inland saltern were studied. the well water leading into the ponds of the saltern differed from sea water in its total salinity (18% (w/v)) and had lower mg2+ and so4(2-) contents and much higher contents of cl-, ca2+- and k+. a high proportion of halotolerant microorganisms were isolated, although marine bacteria were not found. most of the halophilic strains isolated appeared to be typical moderate halophiles, and included si ... | 1987 | 3606874 |
| use of a green fluorescent protein gene as a reporter in zymomonas mobilis and halomonas elongata. | we investigated the applicability of the green fluorescent protein of aequorea victoria as a reporter for gene expression in the strictly fermentative gram-negative ethanologenic bacterium zymomonas mobilis and in the moderately halophilic bacterium halomonas elongata. we have succeeded to express a mutated gene of green fluorescent protein under the control of different promoters in z. mobilis and h. elongata grown under various glucose or salt concentrations, respectively. our results demonstr ... | 2001 | 11470365 |
| changes in the hydrophobic-hydrophilic cell surface character of halomonas elongata in response to nacl. | phase-partitioning studies of the euryhaline bacterium halomonas elongata demonstrated that the hydrophobic-hydrophilic nature of the cell surface changed as the bacterium grew in different nacl concentrations. mid-log-phase cells grown in a high (3.4 m) nacl concentration were more hydrophilic than were cells grown in a low (0.05 m) nacl concentration. mid-log-phase cells from defined medium containing 3.4 m nacl normally produced a hydrophobicity reading of only 14 (hexadecane hydrophobicity = ... | 1988 | 3335480 |
| heat shock proteins in the moderately halophilic bacterium deleya halophila: protective effect of high salt concentration against thermal shock. | the moderately halophilic eubacterium deleya halophila grown in medium containing 1 m or 2.5 m nacl was heat-shocked at various temperatures and the electrophoretic patterns of pulse-labelled proteins were examined. several polypeptides were induced (heat shock proteins, or hsp) at all temperatures. however, the level of induction of some hsp was dependent on the severity of the thermal shock as well as the salt concentration of the growth medium. time course studies revealed that synthesis of s ... | 1988 | 3252902 |
| identification and characterization of bacteria in a selenium-contaminated hypersaline evaporation pond. | solar evaporation ponds are commonly used to reduce the volume of seleniferous agricultural drainage water in the san joaquin valley, calif. these hypersaline ponds pose an environmental health hazard because they are heavily contaminated with selenium (se), mainly in the form of selenate. se in the ponds may be removed by microbial se volatilization, a bioremediation process whereby toxic, bioavailable selenate is converted to relatively nontoxic dimethylselenide gas. in order to identify micro ... | 2001 | 11525968 |
| survey of metal tolerance in moderately halophilic eubacteria. | the tolerance patterns, expressed as mics, for 250 moderately halophilic eubacteria to 10 heavy metals were surveyed by using an agar dilution method. the moderate halophiles tested included 12 culture collection strains and fresh isolates representative of deleya halophila (37 strains), acinetobacter sp. (24 strains), flavobacterium sp. (28 strains), and 149 moderately halophilic gram-positive cocci included in the genera marinococcus, sporosarcina, micrococcus, and staphylococcus. on the basis ... | 1989 | 2802612 |
| analysis of the replication region of the cryptic plasmid phe1 from the moderate halophile halomonas elongata. | the basic replicon of the narrow-host-range plasmid phe1 from the moderately halophilic bacterium halomonas elongata atcc 33174 has been identified and characterized. the replicon consists of a 1.7-kb dna fragment, which contains the genetic information required for autonomous replication and stable maintenance. analysis of its sequence revealed the presence of two orfs which seem to form one transcription unit. orf1 encodes a replication initiator protein (repa), which has a high degree of homo ... | 1999 | 10394923 |
| bacterial milking: a novel bioprocess for production of compatible solutes. | a novel biotechnological process called "bacterial milking" has been established for the production of compatible solutes using the gram-negative bacterium halomonas elongata. following a high-cell-density fermentation which provided biomass up to 48 g cell dry weight per liter, we applied alternating osmotic shocks in combination with crossflow filtration techniques to harvest the compatible solutes ectoine and hydroxyectoine. h. elongata, like other halophilic or halotolerant microorganisms, p ... | 1998 | 10099207 |
| characterization of nucleoside diphosphate kinase from moderately halophilic eubacteria. | nucleoside diphosphate kinase was purified to apparent homogeneity from naturally isolated moderately halophilic eubacteria by atp-agarose and phenyl-5pw column chromatographies. the molecular mass of this enzyme was 15 kda by time-of-flight mass-spectrometry. this protein showed anomalous mobility on sds-page which is typical of a halophilic protein. it was stable and active over a wide range of salt concentrations, from 0 to 4.0 m nacl. | 2001 | 11758938 |
| characterization of the genes for the biosynthesis of the compatible solute ectoine in the moderately halophilic bacterium halomonas elongata dsm 3043. | the ectoine synthesis genes of the moderately halophilic bacterium halomonas elongata dsm 3043 have been precisely located in a 2.8-kb ecori region of a cosmid clone previously isolated (canovas, d., vargas, c., iglesias-guerra, f., csonka, l. n., rhodes, d., ventosa, a., nieto, j. j.: isolation and characterization of salt-sensitive mutants of the moderate halophile halomonas elongata and cloning of the ectoine synthesis genes. j. biol. chem. 272, 25794-25801, 1997). this region was sequenced a ... | 1998 | 9924816 |
| analysis of the genome of the gram-negative moderate halophiles halomonas and chromohalobacter by using pulsed-field gel electrophoresis. | the genomes of 11 moderately halophilic bacteria belonging to the genera halomonas and chromohalobacter have been analyzed by restriction endonuclease digestion and pulsed-field gel electrophoresis (pfge). by using the infrequently cutting restriction endonucleases spei and swai, highly characteristic fingerprintings were obtained for each of the isolates studied. on the basis of the lengths of the spei and swai fragments, separated by pfge, the genome size of the 11 strains studied was estimate ... | 1998 | 9827333 |
| influence of salt concentration on the cellular fatty acid composition of the moderately halophilic bacterium halomonas salina. | the cellular fatty acid composition of halomonas salina, a moderately halophilic bacterium grown at different salt concentrations, is reported. fatty acids c16:0 and c18:1 were major components and significant amounts of c16:1, c18:0 and cyc-c19:0 were also detected. the results showed clear chemotaxonomic relationships with recognized members of the genus halomonas. the salt concentration greatly influenced the fatty acid composition, suggesting activation of cyclopropane synthetase at high lev ... | 1998 | 9826923 |
| growth of moderately halophilic bacteria isolated from sea water using phenol as the sole carbon source. | moderately halophilic bacteria utilizing phenol as the sole carbon source were isolated by selective enrichment from sea water. the isolate (gram-negative motile rods) was identified as deleya venusta. it grew well in the presence of up to 1600 mg/l of phenol and 8% nacl under aerobic conditions. when the cells were treated with chloramphenicol prior to the addition of phenol they did not utilize added phenol, even after prolonged incubation. thus, the enzymes necessary for phenol metabolism app ... | 2001 | 11830940 |
| biomineralization of carbonates by halomonas eurihalina in solid and liquid media with different salinities: crystal formation sequence. | carbonate precipitation by 20 strains of the moderately halophilic species halomonas eurihalina in both solid and liquid media was studied. the influence of salinity and temperature on the quantity and type of crystals precipitated was also investigated. some strains of h. eurihalina formed crystals in all conditions tested. the mineral phases precipitated were magnesium calcite, aragonite and monohydrocalcite in variable proportions depending on various factors such as the type of growth medium ... | 1998 | 9766229 |
| the effects of ultraviolet radiation on the moderate halophile halomonas elongata and the extreme halophile halobacterium salinarum. | both the moderately halophilic bacterium, halomonas elongata, and the extremely halophilic archaea, halobacterium salinarum, can be found in hypersaline environments (e.g., salterns). on complex media, h. elongata grows over a salt range of 0.05-5.2 m, whereas, h. salinarum multiplies over a salt range of 2.5-5.2 m. the purpose of this study was to illustrate the effect that solar (uv-a and uv-b) and germicidal radiation (uv-c) had on the growth patterns of these bacteria at varied salt concentr ... | 2000 | 10721487 |
| transposon mutagenesis in halomonas eurihalina. | we have established a transposon mutagenesis procedure for the moderate halophile halomonas eurihalina, a bacteria that produces an exopolysaccharide (eps) of considerable biotechnological interest. we used suicide plasmids put and psup102 to introduce the transposons mini-tn5 and tn1732 into h. eurihalina via escherichia coli mediated conjugation. southern hybridization analysis demonstrated that insertions of the transposon mini-tn5 into h. eurihalina occurred randomly at single sites in the c ... | 2000 | 10724479 |
| screening and characterization of aldehyde dehydrogenase gene from halomonas salina strain as11. | a population survey was made of moderately halophilic bacteria in prawn pond sediment in the songkla region of thailand. twenty-two isolated halophilic bacteria capable of growing on modified atcc culture medium 1270 for halobacterium were then assayed for aldehyde dehydrogenase (aldh) activity which might be involved in the metabolism of xenobiotic compounds. one isolate, designated as11, was selected based on its high amount of aldh activity. this organism can grow at sodium chloride concentra ... | 2002 | 11911926 |
| analysis and expression of algl, which encodes alginate lyase in pseudomonas syringae pv. syringae. | pseudomonas syringae produces alginate, an exopolysaccharide that contributes to the virulence and epiphytic fitness of this phytopathogenic bacterium. p. syringae also produces the algl-encoded alginate lyase, which cleaves the alginate biopolymer via a beta-elimination reaction. the algl gene from p. syringae maps to a 1134 bp region within the alginate biosynthetic operon, and is similar to algl from halomonas marina, p. aeruginosa, azotobacter chroococcum, and a. vinelandii. algl from p. syr ... | 2001 | 11913796 |
| effect of exopolysaccharide v2-7, isolated from halomonas eurihalina, on the proliferation in vitro of human peripheral blood lymphocytes. | the immunomodulatory activity of the exopolysaccharide v2-7, a sulfated polymer excreted by the moderately halophilic bacteria halomonas eurihalina, was studied in vitro. [3h]thymidine incorporation and flow-cytometry measurements showed that this exopolysaccharide enhanced the unspecific proliferation of human lymphocytes in response to the presence of anti-cd3 monoclonal antibody. it was effective at concentrations of less than 1 microg/ml, maximum activity being achieved at 0.2 microg/ml. | 2000 | 10737262 |
| halomonas magadii sp. nov., a new member of the genus halomonas, isolated from a soda lake of the east african rift valley. | a number of novel alkaliphilic organotrophic bacteria have been isolated from several saline and alkaline east african soda lakes. the new isolates grow at ph values between 7.0 and 11.0, with ph optima for growth between 9.0 and 10.0. growth occurs at total salts concentration between 0% and 20% (w/v) with optimum at 0%-7% (w/v). phylogenetic analyses based on 16s rdna sequence comparison indicate that these isolates are related (>96% similarity) to members of the halomonadaceae within the gamm ... | 2000 | 10741837 |
| new type of osmoregulated solute transporter identified in halophilic members of the bacteria domain: trap transporter teaabc mediates uptake of ectoine and hydroxyectoine in halomonas elongata dsm 2581(t). | the halophilic bacterium halomonas elongata synthesizes as its main compatible solute the aspartate derivative ectoine. we constructed a deletion mutant of h. elongata, kb1, defective in ectoine synthesis and tolerating elevated salt concentrations only in the presence of external compatible solutes. the dependency of kb1 on solute uptake for growth in high-salt medium was exploited to select insertion mutants unable to accumulate external solutes via osmoregulated transporters. one insertion mu ... | 2002 | 12003950 |
| two species of culturable bacteria associated with degradation of brown algae fucus evanescens. | the heterotrophic microbial enrichment community established during degradation of brown algae fucus evanescens was characterized. a two-species bacterial community of marine culturable gamma-proteobacteria consisted of pseudoalteromonas and halomonas. the first member of the community, pseudoalteromonas sp., was highly metabolically active, had bacteriolytic and hemolytic activities, produced proteinases (gelatinase and caseinase), lipases, dnases, and fucoidanhydrolases, laminaranases, alginas ... | 2002 | 12023731 |
| impact of irradiation and polycyclic aromatic hydrocarbon spiking on microbial populations in marine sediment for future aging and biodegradability studies. | experiments were carried out to develop methods to generate well-characterized, polycyclic aromatic hydrocarbon (pah)-spiked, aged but minimally altered sediments for fate, biodegradation, and bioavailability experiments. changes in indigenous bacterial populations were monitored in mesocosms constructed of relatively clean san diego bay sediments, with and without exposure to gamma radiation, and then spiked with five different pahs and hexadecane. while phenanthrene and chrysene degraders were ... | 2002 | 12039743 |
| the substrate-binding protein teaa of the osmoregulated ectoine transporter teaabc from halomonas elongata: purification and characterization of recombinant teaa. | teaabc is a novel, osmoregulated secondary transport system for compatible solutes found in the halophilic proteobacterium halomonas elongata dsm 2581(t). sequence comparisons have confirmed that teaabc belongs to the family of trap transporters and as such it consists of two putative transmembrane proteins (teab, teac) and a putative periplasmic substrate-binding protein (teaa). teaabc is the only osmoregulated transporter for ectoines found in h. elongata. by overexpressing teaa-(6)his tag in ... | 2002 | 12076815 |
| purification, characterization, and gene sequencing of a catalase from an alkali- and halo-tolerant bacterium, halomonas sp. sk1. | an alkali- and halo-tolerant bacterium with high catalase activity was isolated and identified as a new species of the genus halomonas. its catalase (hkta) was simply purified by two steps of liquid chromatography. a 71.4% yield of the catalase was obtained with 97% purity on sds-page. the specific activity of hkta (57,900 u/mg protein) was two times higher than that of bovine liver catalase. the purified enzyme is inhibited by kcn, nh2oh, nan3, and 3-amino-1,2,4-triazole, active at ph 5.0-11.0, ... | 2002 | 12092846 |
| use of pcr-rflp for genotyping 16s rrna and characterizing bacteria cultured from halibut fry. | small subunit ribosomal genes were explored using pcr-rflp to facilitate the characterization of bacteria cultured from reared fry of the atlantic halibut (hippoglossus hippoglossus). concern has been expressed about pathogen invasion in larvae lacking a counteracting normal flora that may aid the immune system in producing robust noninfected individuals. in this study, pure cultured representatives of normal flora that were previously found to be antagonistic towards a pathogenic vibrio sp. wer ... | 2002 | 12109877 |
| analysis of the genome of the moderate halophile halomonas eurihalina. | we have studied the genomic organization of halomonas eurihalina, a moderately halophilic bacterium that produces an anionic exopolysaccharide with a potentially wide range of applications in industry. to estimate the genome size of strain f2-7, large restriction fragments of genomic dna were separated by pulsed-field gel electrophoresis. according to the average size of the resolved restriction fragments, the genome size of h. eurihalina strain f2-7 was estimated to be around 2500 kb. the physi ... | 2002 | 12192518 |
| cloning and expression of alpha-amylase from the hyperthermophilic archaeon pyrococcus woesei in the moderately halophilic bacterium halomonas elongata. | an extracellular alpha-amylase gene from the hyperthermophilic archaeon pyrococcus woesei has been cloned and sequenced. the 1.4-kb protein-coding sequence is identical to that of the corresponding alpha-amylase gene of the closely related species p. furiosus. by using a shuttle cloning vector for halophilic bacteria, the p. woesei alpha-amylase was expressed in the moderate halophile halomonas elongata, under the control of a native h. elongata promoter. the hyperthermophilic amylase activity e ... | 2000 | 10747230 |
| halomonas campisalis sp. nov., a denitrifying, moderately haloalkaliphilic bacterium. | the isolation and characterization of a denitrifying bacterium that is both moderately halophilic and alkaliphilic is described. the organism was isolated for use in the development of a bioprocess that could potentially reduce the costs of ion exchange resin regenerant disposal. the process of ion exchange, after resin regeneration, produces a briny, alkaline waste that is difficult and expensive to dispose. the biological removal of nitrate and subsequent reuse of these brines can potentially ... | 1999 | 10794143 |
| high incidence of halotolerant bacteria in pacific hydrothermal-vent and pelagic environments. | the abundance of halotolerant microorganisms in hydrothermal-vent and pelagic waters in the north and south pacific was estimated by the most probable number (mpn) technique using a heterotrophic 16% nacl medium incubated at 20-24 degrees c. based on these mpns and direct counts with epifluorescence microscopy to enumerate the total microbial population, salt-tolerant microbes comprised from <0.01 to >28% of the total microbial community. fourteen isolates from these mpn enrichments were identif ... | 2000 | 10858584 |
| diversity of thiosulfate-oxidizing bacteria from marine sediments and hydrothermal vents. | species diversity, phylogenetic affiliations, and environmental occurrence patterns of thiosulfate-oxidizing marine bacteria were investigated by using new isolates from serially diluted continental slope and deep-sea abyssal plain sediments collected off the coast of new england and strains cultured previously from galapagos hydrothermal vent samples. the most frequently obtained new isolates, mostly from 10(3)- and 10(4)-fold dilutions of the continental slope sediment, oxidized thiosulfate to ... | 2000 | 10919760 |
| human infection with halomonas venusta following fish bite. | halomonas venusta, a moderately halophilic, nonfermentative gram-negative rod, is reported for the first time as a human pathogen in a wound that originated from a fish bite. | 2000 | 10921995 |
| characteristics of bioemulsifiers synthesised in crude oil media by halomonas eurihalina and their effectiveness in the isolation of bacteria able to grow in the presence of hydrocarbons. | halomonas eurihalina strains f2-7, h28, h96, h212 and h214 were capable of producing large amounts of exopolysaccharides (eps) in my medium with added crude oil. the biopolymers showed lower carbohydrate and protein content than those synthesised in control medium without oil. nevertheless, the percentages of uronic acids, acetyls and sulphates were higher. the emulsifying activity of biopolymers was measured; crude oil was the substrate most efficiently emulsified. furthermore, all the eps test ... | 2002 | 12436318 |
| artificial neural network based identification of environmental bacteria by gas-chromatographic and electrophoretic data. | chemotaxonomic identification techniques are powerful tools for environmental micro-organisms, for which poor diagnostic schemes are available. whole cellular fatty acid methyl esters (fame) content is a stable bacterial profile, the analysis method is rapid, cheap, simple to perform and highly automated. whole-cell protein is an even more powerful tool because it yields information at or below the species level. the description of new species and genera and subsequent continuous rearrangement p ... | 2000 | 11084227 |
| polyamine distribution patterns within the families aeromonadaceae, vibrionaceae, pasteurellaceae, and halomonadaceae, and related genera of the gamma subclass of the proteobacteria. | polyamines of the four families and the five related genera within the gamma subclass of the class proteobacteria were analyzed by hplc with the objective of developing a chemotaxonomic system. the production of putrescine, diaminopropane, cadaverine, and agmatine are not exactly correlated to the phylogenetic genospecies within 36 strains of the genus aeromonas (the family aeromonadaceae) lacking in triamines. the occurrence of norspermidine was limited but not ubiquitous within the family vibr ... | 1997 | 12501353 |
| halomonas muralis sp. nov., isolated from microbial biofilms colonizing the walls and murals of the saint-catherine chapel (castle herberstein, austria). | a group of seven halophilic strains (optimal growth at 2.5-10.0% nacl) was isolated from samples of a wall and a mural painting, both heavily contaminated by microbial growth, inside the saint-catherine chapel of castle herberstein (austria). the strains were subjected to a polyphasic taxonomic study that included dna-dna relatedness studies, dna base-ratio determinations, 16s rdna sequence analysis, rep-pcr genomic fingerprinting, fatty acid analysis and phenotypic and biochemical characterizat ... | 2002 | 12508867 |
| microbial thiocyanate utilization under highly alkaline conditions. | three kinds of alkaliphilic bacteria able to utilize thiocyanate (cns-) at ph 10 were found in highly alkaline soda lake sediments and soda soils. the first group included obligate heterotrophs that utilized thiocyanate as a nitrogen source while growing at ph 10 with acetate as carbon and energy sources. most of the heterotrophic strains were able to oxidize sulfide and thiosulfate to tetrathionate. the second group included obligately autotrophic sulfur-oxidizing alkaliphiles which utilized th ... | 2001 | 11157213 |
| diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes. | the aim of this study was to determine the diversity of moderately halophilic bacteria with hydrolase activities. | 2003 | 12534822 |
| [analysis of 16s rdna sequences and dna-dna hybridization of moderately halophilic bacteria from xinjiang region]. | based on the previous studies on numerical taxonomy and 16s rdna pcr-rflp analysis, the moderately halophilic bacteria isolated from xinjiang region constituted a new cluster, and the phylogenetic tree was constructed by comparing with the 16s rdna sequences of the other moderately halophilic bacteria species. in the phylogenetic tree, most of the reference strains were clustered in a group, and the similarity values of 16s rdna sequence were above 96%. however, ai-3, alcanivorax borkumensis and ... | 2002 | 12557387 |