| marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a new, extremely halotolerant, hydrocarbon-degrading marine bacterium. | on the basis of phenotypical characteristics and analysis of 16s rrna sequence, a new species belonging to a new genus is described, and the name marinobacter hydrocarbonoclasticus is proposed. this organism, isolated from mediterranean seawater near a petroleum refinery, is a gram-negative, aerobic, rod-shaped bacterium. it grows at nacl concentrations of 0.08 to 3.5 m and uses various hydrocarbons as the sole source of carbon and energy. its dna has a guanine-plus-cytosine content of 52.7 mol% ... | 1992 | 1382536 |
| diversity and association of psychrophilic bacteria in antarctic sea ice. | the bacterial populations associated with sea ice sampled from antarctic coastal areas were investigated by use of a phenotypic approach and a phylogenetic approach based on genes encoding 16s rrna (16s rdna). the diversity of bacteria associated with sea ice was also compared with the bacterial diversity of seawater underlying sea ice. psychrophilic (optimal growth temperature, < or = 15 degrees c; no growth occurring at 20 degrees c) bacterial diversity was found to be significantly enriched i ... | 1997 | 9251193 |
| fundibacter jadensis gen. nov., sp. nov., a new slightly halophilic bacterium, isolated from intertidal sediment. | a moderately halophilic hydrocarbon-degrading bacterium was isolated from continuous cultures containing a suspension of intertidal sediment from the german north sea coast with hexadecane as the sole carbon source. on the basis of phenotypic characteristics, fatty acid analysis and 16s rdna sequence analysis, it was considered to be a new species belonging to a new genus. it is a gram-negative, aerobic, rod-shaped bacterium, whose cell size varies. it grows at concentrations of 0.5-15% (w/v) na ... | 1999 | 10319463 |
| marinobacter strain nce312 has a pseudomonas-like naphthalene dioxygenase. | one strain of bacteria, designated nce312, was isolated from a naphthalene-digesting chemostat culture that was inoculated with creosote-contaminated marine sediment. the strain was isolated based on its ability to grow using naphthalene as a sole carbon source. in addition, the strain degraded 2-methylnaphthalene and 1-methylnaphthalene. analysis of a 16s rrna gene sequence from nce312 placed the isolate in the genus marinobacter. degenerate pcr primers were used to amplify a fragment of a naph ... | 2001 | 11445166 |
| impact of irradiation and polycyclic aromatic hydrocarbon spiking on microbial populations in marine sediment for future aging and biodegradability studies. | experiments were carried out to develop methods to generate well-characterized, polycyclic aromatic hydrocarbon (pah)-spiked, aged but minimally altered sediments for fate, biodegradation, and bioavailability experiments. changes in indigenous bacterial populations were monitored in mesocosms constructed of relatively clean san diego bay sediments, with and without exposure to gamma radiation, and then spiked with five different pahs and hexadecane. while phenanthrene and chrysene degraders were ... | 2002 | 12039743 |
| polyamine distribution patterns within the families aeromonadaceae, vibrionaceae, pasteurellaceae, and halomonadaceae, and related genera of the gamma subclass of the proteobacteria. | polyamines of the four families and the five related genera within the gamma subclass of the class proteobacteria were analyzed by hplc with the objective of developing a chemotaxonomic system. the production of putrescine, diaminopropane, cadaverine, and agmatine are not exactly correlated to the phylogenetic genospecies within 36 strains of the genus aeromonas (the family aeromonadaceae) lacking in triamines. the occurrence of norspermidine was limited but not ubiquitous within the family vibr ... | 1997 | 12501353 |
| diversity of 746 heterotrophic bacteria isolated from microbial mats from ten antarctic lakes. | microbial mats, growing in antarctic lakes constitute unique and very diverse habitats. in these mats microorganisms are confronted with extreme life conditions. we isolated 746 bacterial strains from mats collected from ten lakes in the dry valleys (lakes hoare and fryxell), the vestfold hills (lakes ace, druzhby, grace, highway, pendant, organic and watts) and the larsemann hills (lake reid), using heterotrophic growth conditions. these strains were investigated by fatty acid analysis, and by ... | 2002 | 12583721 |
| isolation and characterization of novel psychrophilic, neutrophilic, fe-oxidizing, chemolithoautotrophic alpha- and gamma-proteobacteria from the deep sea. | we report the isolation and physiological characterization of novel, psychrophilic, iron-oxidizing bacteria (feob) from low-temperature weathering habitats in the vicinity of the juan de fuca deep-sea hydrothermal area. the feob were cultured from the surfaces of weathered rock and metalliferous sediments. they are capable of growth on a variety of natural and synthetic solid rock and mineral substrates, such as pyrite (fes(2)), basalt glass ( approximately 10 wt% feo), and siderite (feco(3)), a ... | 2003 | 12732565 |
| determination of dna content of aquatic bacteria by flow cytometry. | the distribution of dna among bacterioplankton and bacterial isolates was determined by flow cytometry of dapi (4',6'-diamidino-2-phenylindole)-stained organisms. conditions were optimized to minimize error from nonspecific staining, at bias, dna packing, changes in ionic strength, and differences in cell permeability. the sensitivity was sufficient to characterize the small 1- to 2-mb-genome organisms in freshwater and seawater, as well as low-dna cells ("dims"). the dims could be formed from l ... | 2001 | 11282616 |
| metabolic differences between attached and free-living marine bacteria: inadequacy of liquid cultures for describing in situ bacterial activity. | marinobacter sp. strain cab was cultivated with or without porous glass beads as solid support. two substrates were used: the hydrophilic sodium lactate and a hydrophobic c(18)-isoprenoid ketone (6,10,14-trimethylpentadecan-2-one (tmp)). the substrate adsorption onto the beads was measured. bacterial adhesion was determined by a direct count technique and amounted to 70% of total cells. in the immobilised cell cultures (icc), generation times were 1.5 and 1.8 times shorter than in the planktonic ... | 2001 | 11150675 |
| oxygen-dependent growth of the sulfate-reducing bacterium desulfovibrio oxyclinae in coculture with marinobacter sp. strain mb in an aerated sulfate-depleted chemostat. | a chemostat coculture of the sulfate-reducing bacterium desulfovibrio oxyclinae and the facultatively aerobic heterotroph marinobacter sp. strain mb was grown for 1 week under anaerobic conditions at a dilution rate of 0.05 h(-1). it was then exposed to an oxygen flux of 223 micromol min(-1) by gassing the growth vessel with 5% o(2). sulfate reduction persisted under these conditions, though the amount of sulfate reduced decreased by 45% compared to the amount reduced during the initial anaerobi ... | 2000 | 11055958 |
| self-assembling amphiphilic siderophores from marine bacteria. | most aerobic bacteria secrete siderophores to facilitate iron acquisition. two families of siderophores were isolated from strains belonging to two different genera of marine bacteria. the aquachelins, from halomonas aquamarina strain ds40m3, and the marinobactins, from marinobacter sp. strains ds40m6 and ds40m8, each contain a unique peptidic head group that coordinates iron(iii) and an appendage of one of a series of fatty acid moieties. these siderophores have low critical micelle concentrati ... | 2000 | 10678827 |
| marinobacter aquaeolei sp. nov., a halophilic bacterium isolated from a vietnamese oil-producing well. | several strains of moderately halophilic and mesophilic bacteria were isolated at the head of an oil-producing well on an offshore platform in southern vietnam. cells were gram-negative, non-spore-forming, rod-shaped and motile by means of a polar flagellum. growth occurred at nacl concentrations between 0 and 20%; the optimum was 5% nacl. one strain, which was designated vt8t, could degrade n-hexadecane, pristane and some crude oil components. it grew anaerobically in the presence of nitrate on ... | 1999 | 10319457 |
| production of wax esters during aerobic growth of marine bacteria on isoprenoid compounds | this paper describes the production of isoprenoid wax esters during the aerobic degradation of 6,10,14-trimethylpentadecan-2-one and phytol by four bacteria (acinetobacter sp. strain phy9, pseudomonas nautica [ip85/617], marinobacter sp. strain cab [dsmz 11874], and marinobacter hydrocarbonoclasticus [atcc 49840]) isolated from the marine environment. different pathways are proposed to explain the formation of these compounds. in the case of 6,10, 14-trimethylpentadecan-2-one, these esters resul ... | 1999 | 9872783 |
| aerobic and anaerobic metabolism of 6,10,14-trimethylpentadecan-2-one by a denitrifying bacterium isolated from marine sediments. | this report describes the metabolism of 6,10,14-trimethylpentadecan-2-one by a denitrifying bacterium (marinobacter sp. strain cab) isolated from marine sediments. under aerobic and denitrifying conditions, this strain efficiently degraded this ubiquitous isoprenoid ketone. several bacterial metabolites, 4,8,12-trimethyl-tridecan-1-ol, 4,8,12-trimethyltridecanal, 4,8,12-trimethyltridecanoic acid, z-3,7-dimethylocten-2-oic acid, z-3,7,11-trimethyldodecen-2-oic acid, and 6,10,14-trimethylpentadeca ... | 1997 | 9023941 |
| diversity and structure of bacterial communities in arctic versus antarctic pack ice. | a comprehensive assessment of bacterial diversity and community composition in arctic and antarctic pack ice was conducted through cultivation and cultivation-independent molecular techniques. we sequenced 16s rrna genes from 115 and 87 pure cultures of bacteria isolated from arctic and antarctic pack ice, respectively. most of the 33 arctic phylotypes were >97% identical to previously described antarctic species or to our own antarctic isolates. at both poles, the alpha- and gamma-proteobacteri ... | 2003 | 14602620 |
| competition for polymers among heterotrophic bacteria, isolated from particles of the equatorial atlantic. | three heterotrophic bacterial strains, isolated from organic particles of the upper water column of the equatorial atlantic, taken during a cruise on the r/v meteor (1997), were investigated concerning their physiological and phylogenetic properties using classic microbiological and modern molecular-biological methods. all isolates are gram-negative rods able to use polymers such as cellulose, chitin or starch as sole carbon source. the phylogeny of these isolates was investigated by fluorescenc ... | 2004 | 15179602 |
| marinobacter lipolyticus sp. nov., a novel moderate halophile with lipolytic activity. | in the course of a screening programme in hypersaline habitats of southern spain to isolate halophilic bacteria that are able to produce different extracellular enzymes, a novel, moderately halophilic bacterium (strain sm19(t)) that displays lipolytic activity has been isolated and characterized. strain sm19(t) is a gram-negative rod that grows optimally in culture media that contain 7.5 % nacl. the dna g+c content was 57.0 mol%. according to phenotypic and genotypic data, this strain was assign ... | 2003 | 13130022 |
| marinobacter lutaoensis sp. nov., a thermotolerant marine bacterium isolated from a coastal hot spring in lutao, taiwan. | a heterotrophic and thermotolerant marine bacterium, designated strain t5054, was isolated from a hot spring on the coast of lutao, taiwan. it was a strictly aerobic, gram-negative rod. cells grown in broth cultures were non-spore-forming and motile by means of one to several polar flagella. it seems that pilus-like structures were produced from both poles of the cells. strain t5054 required na+ for growth and exhibited optimal growth at about 45 degrees c, ph 7, and 3-5% nacl. it contained iso- ... | 2003 | 12897833 |
| production of a polyunsaturated isoprenoid wax ester during aerobic metabolism of squalene by marinobacter squalenivorans sp. nov. | this paper describes the production of 5,9,13-trimethyltetradeca-4e,8e,12-trienyl-5,9,13-trimethyltetradeca-4e,8e,12-trienoate during the aerobic degradation of squalene by a marinobacter strain, 2asq64, isolated from the marine environment. a pathway involving initial cleavage of the c(10)-c(11) or c(14)-c(15) double bonds of the squalene molecule is proposed to explain the formation of this polyunsaturated isoprenoid wax ester. the isoprenoid wax ester content reached 1.1% of the degraded squa ... | 2003 | 12839795 |
| oleispira antarctica gen. nov., sp. nov., a novel hydrocarbonoclastic marine bacterium isolated from antarctic coastal sea water. | the taxonomic characteristics of two bacterial strains, rb-8(t) and rb-9, isolated from hydrocarbon-degrading enrichment cultures obtained from antarctic coastal marine environments (rod bay, ross sea), were determined. these bacteria were psychrophilic, aerobic and gram-negative with polar flagella. growth was not observed in the absence of nacl, occurred only at concentrations of na+ above 20 mm and was optimal at an nacl concentration of 3-5% (w/v). the major cellular fatty acids were monouns ... | 2003 | 12807200 |
| marinobacter litoralis sp. nov., a moderately halophilic bacterium isolated from sea water from the east sea in korea. | a gram-negative, motile, non-spore-forming and moderately halophilic rod-shaped strain, sw-45t, was isolated from sea water of the east sea in korea. the organism grew optimally at 30-37 degrees c and grew at 4 and 46 degrees c. it grew in the presence of 0.5-18% (v/w) nacl, with an optimum of 2-7% nacl. strain sw-45t was chemotaxonomically characterized by having ubiquinone-9 (q-9) as the major respiratory lipoquinone and c16 : 0, c18 : 1omega9c and c16 : 1omega9c as the predominant fatty acids ... | 2003 | 12710627 |
| petrobactin sulfonate, a new siderophore produced by the marine bacterium marinobacter hydrocarbonoclasticus. | culture of the oil-degrading marine bacterium marinobacter hydrocarbonoclasticus gave the known siderophore petrobactin (1) and the new metabolite petrobactin sulfonate (2), the first marine siderophore containing a sulfonated 3,4-dihydroxy aromatic ring. the structure of petrobactin sulfonate was elucidated from spectral data, resulting in a revision of the nmr assignments of petrobactin. | 2004 | 15568785 |
| bioremediation of oil by marine microbial mats. | cyanobacterial mats developing in oil-contaminated sabkhas along the african coasts of the gulf of suez and in the pristine solar lake, sinai, were collected for laboratory studies. samples of both mats showed efficient degradation of crude oil in the light, followed by development of an intense bloom of phormidium spp. and oscillatoria spp. isolated cyanobacterial strains, however, did not degrade crude oil in axenic cultures. strains of sulfate-reducing bacteria and aerobic heterotrophs were c ... | 2002 | 12497184 |
| aerobic and anaerobic metabolism of squalene by a denitrifying bacterium isolated from marine sediment. | the aerobic and anaerobic metabolism of the isoprenoid alkene squalene was investigated in a new type of marine denitrifying bacterium, strain 2sq31, isolated from marine sediment. strain 2sq31 was identified as a species of marinobacter. under denitrifying conditions, the strain efficiently degraded squalene; of 0.7 mmol added per liter of medium, 77% was degraded within 120 days under anoxic conditions with nitrate as electron acceptor. tertiary diols and methyl ketones were identified as meta ... | 2002 | 12209261 |
| metabolism of diphenylurea by a marinobacter sp. isolated from a contaminated ephemeral stream bed in the negev desert. | a moderate halophilic marinobacter sp. (designated strain dpuz) able to metabolize 1,3-diphenylurea (dpu) was isolated from a contaminated ephemeral desert stream bed near an industrial complex in the northern part of the negev desert (israel). metabolism of dpu was accompanied by a transient accumulation of a metabolite identified as aniline using gas chromatography-mass spectrometry, thus indicating a metabolic pathway involving cleavage of the urea bridge between the phenyl structures. anilin ... | 2002 | 12167538 |
| morphological, small subunit rrna, and physiological characterization of trimyema minutum (kahl, 1931), an anaerobic ciliate from submarine hydrothermal vents growing from 28 degrees c to 52 degrees c. | a thermophilic strain of trimyema minutum was isolated from the hydrothermally heated sea floor at vulcano island (italy) and cultivated monoxenically on marinobacter sp. and methanococcus thermolithotrophicus. it can be propagated strictly anaerobically and is sensitive to oxygen: if exposed to air at 48 degrees c all cells die within 60 min. it grows from 0.45-7.2% (w/v) salt and at ph 6.0-8.0. the isolate is the most extreme thermophilic ciliate which ever has been cultivated, exhibiting an o ... | 2002 | 12120988 |
| oleiphilaceae fam. nov., to include oleiphilus messinensis gen. nov., sp. nov., a novel marine bacterium that obligately utilizes hydrocarbons. | a bacterial isolate, me102t, was obtained from an n-hexadecane enrichment culture of seawater/sediment samples collected in the harbour of messina (italy). this gram-negative, aerobic, motile, rod-shaped bacterium used a narrow spectrum of organic compounds, including aliphatic hydrocarbons, alkanoates and alkanoles, as carbon and energy sources. none of the sugars, organic acids or amino acids tested was used. during cultivation on n-alkanes as the sole source of carbon and energy, the cells fo ... | 2002 | 12054256 |
| tolerance to cadmium of free-living and associated with marine animals and eelgrass marine gamma-proteobacteria. | the tolerance to cd2+ and possible mechanisms of cd2+ detoxification by 178 free-living bacteria isolated from sea water, associated with marine animals (a mussel crenomytilus grayanus, a scallop patinopecten yessoensis), and eelgrass zostera marina collected in the sea of japan and the sea of okhotsk have been studied. the concentrations of 25 and 50 mg cd2+/l were highly toxic and inhibited the growth from 54% to 78% of the total bacteria studied. the free-living bacteria isolated from seawate ... | 2002 | 11927987 |
| bacterial deposition in a parallel plate and a stagnation point flow chamber: microbial adhesion mechanisms depend on the mass transport conditions. | deposition onto glass in a parallel plate (pp) and in a stagnation point (sp) flow chamber of marinobacter hydrocarbonoclasticus, psychrobacter sp. and halomonas pacifica, suspended in artificial seawater, was compared in order to determine the influence of methodology on bacterial adhesion mechanisms. the three strains had different cell surface hydrophobicities, with water contact angles on bacterial lawns ranging from 18 to 85 degrees. bacterial zeta potentials in artificial seawater were ess ... | 2002 | 11832522 |
| petrobactin, a photoreactive siderophore produced by the oil-degrading marine bacterium marinobacter hydrocarbonoclasticus. | petrobactin is a bis-catecholate, alpha-hydroxy acid siderophore produced by the oil-degrading marine bacterium marinobacter hydrocarbonoclasticus. the fe(iii)-complexed form of petrobactin is photoreactive in natural sunlight, mediated by the fe(iii)-citrate moiety. the reaction results in decarboxylation of the petrobactin ligand and reduction of fe(iii) to fe(ii). this report is one of the first to show the photoreactivity of fe(iii)-siderophores mediated by the ferric ion-alpha-hydroxy acid ... | 2002 | 11792199 |
| identification of a marine benthic p(3hb)-degrading bacterium isolate and characterization of its p(3hb) depolymerase. | a poly[(r)-3-hydroxybutyrate] (p(3hb))-degrading marine bacterium (strain nk-1, jcm10458) was isolated from the pacific ocean deep-sea floor (1165 m in depth) in japan. the organism was a motile and gram negative, aerobic, and rod-shaped bacterium, and its dna had a guanine-plus-cytosine content of 57.7 mol%. on the basis of several phenotypic characters and a phylogenetic analysis of the gene coding for 16s rrna, this strain was identified as marinobacter sp. the strain required sodium salt for ... | 2000 | 11710100 |
| influence of n-alkanes and petroleum on fatty acid composition of a hydrocarbonoclastic bacterium: marinobacter hydrocarbonoclasticus strain 617. | this study concerns the effects of various long-chain n-alkanes, n-alkane mixtures and arabian light crude oil on the fatty acid (fa) composition of a sedimentary marine bacteria (marinobacter hydrocarbonoclasticus strain 617), growing under aerobic conditions. the cultures with n-alkanes, as compared with soluble carbon sources, led to greater amounts of saturated and methyl branched fa (mainly belonging to a delta10 series). we observed the appearance or increase of saturated and unsaturated f ... | 2001 | 11482638 |
| cloning, expression and characterization of a poly(3-hydroxybutyrate) depolymerase from marinobacter sp. nk-1. | a dna fragment carrying the gene encoding poly(3-hydroxybutyrate) (p(3hb)) depolymerase was cloned from the genomic dna of marinobacter sp. dna sequencing analysis revealed that the marinobacter sp. p(3hb) depolymerase gene is composed of 1734bp and encodes 578 amino acids with a molecular mass of 61,757da. a sequence homology search showed that the deduced protein contains the signal peptide, catalytic domain (cd), cadherin-type linker domain (ld), and two substrate-binding domain (sbd). the fu ... | 2003 | 14607367 |
| sulfate reduction and possible aerobic metabolism of the sulfate-reducing bacterium desulfovibrio oxyclinae in a chemostat coculture with marinobacter sp. strain mb under exposure to increasing oxygen concentrations. | a chemostat coculture of the sulfate-reducing bacterium desulfovibrio oxyclinae together with a facultative aerobe heterotroph tentatively identified as marinobacter sp. strain mb was grown under anaerobic conditions and then exposed to a stepwise-increasing oxygen influx (0 to 20% o(2) in the incoming gas phase). the coculture consumed oxygen efficiently, and no residual oxygen was detected with an oxygen supply of up to 5%. sulfate reduction persisted at all levels of oxygen input, even at the ... | 2000 | 11055957 |
| high incidence of halotolerant bacteria in pacific hydrothermal-vent and pelagic environments. | the abundance of halotolerant microorganisms in hydrothermal-vent and pelagic waters in the north and south pacific was estimated by the most probable number (mpn) technique using a heterotrophic 16% nacl medium incubated at 20-24 degrees c. based on these mpns and direct counts with epifluorescence microscopy to enumerate the total microbial population, salt-tolerant microbes comprised from <0.01 to >28% of the total microbial community. fourteen isolates from these mpn enrichments were identif ... | 2000 | 10858584 |
| multiple linear regression analysis of bacterial deposition to polyurethane coatings after conditioning film formation in the marine environment. | many studies have shown relationships of substratum hydrophobicity, charge or roughness with bacterial adhesion, although bacterial adhesion is governed by interplay of different physico-chemical properties and multiple regression analysis would be more suitable to reveal mechanisms of bacterial adhesion. the formation of a conditioning film of organic compounds adsorbed from seawater affects the properties of substratum surfaces prior to bacterial adhesion, which is a complicating factor in stu ... | 2004 | 15184564 |
| marinobacter alkaliphilus sp. nov., a novel alkaliphilic bacterium isolated from subseafloor alkaline serpentine mud from ocean drilling program site 1200 at south chamorro seamount, mariana forearc. | novel alkaliphilic, mesophilic bacteria were isolated from subseafloor alkaline serpentine mud from the ocean drilling program (odp) hole 1200d at a serpentine mud volcano, south chamorro seamount in the mariana forearc. the cells of type strain odp1200d-1.5t were motile rods with a single polar flagellum. growth was observed between 10 and 45-50 degrees c (optimum temperature: 30-35 degrees c, 45-min doubling time), between ph 6.5 and 10.8-11.4 (optimum: ph 8.5-9.0), and between nacl concentrat ... | 2005 | 15322951 |
| experimental and theoretical bases of specific affinity, a cytoarchitecture-based formulation of nutrient collection proposed to supercede the michaelis-menten paradigm of microbial kinetics. | a theory for solute uptake by whole cells was derived with a focus on the ability of oligobacteria to sequester nutrients. it provided a general relationship that was used to obtain the kinetic constants for in situ marine populations in the presence of naturally occurring substrates. in situ affinities of 0.9 to 400 liters g of cells(-1) h(-1) found were up to 10(3) times smaller than those from a "marinobacter arcticus " isolate, but springtime values were greatly increased by warming. affinit ... | 2004 | 15345439 |
| characteristics of bacteria showing high denitrification activity in saline wastewater. | denitrification efficiency at 10% salinity was compared with that at 2% salinity. the characteristics of bacterial strains isolated from the denitrification system, where an improvement of denitrification efficiency was observed at a high salinity were investigated. | 2006 | 16478517 |
| effects of nitrate treatment on a mixed species, oil field microbial biofilm. | biofilms of bacteria, indigenous to oil field produced water, were grown in square section, glass capillary flow cells at 45 degrees c. initially, in situ image analysis microscopy revealed predominantly coccoid bacteria (length-to-width ratio measurements (l (c):w (c)) of bacterial cells gave a mean value of 1.1), while chemical measurements confirmed sulphate reduction and sulphide production. after nitrate ion addition at 100 and 80 mg/l, in the two repeat experiments respectively, the domina ... | 2006 | 16491355 |
| marinobacter flavimaris sp. nov. and marinobacter daepoensis sp. nov., slightly halophilic organisms isolated from sea water of the yellow sea in korea. | two gram-negative, motile, non-spore-forming and slightly halophilic rods (strains sw-145t and sw-156t) were isolated from sea water of the yellow sea in korea. strains sw-145t and sw-156t grew optimally at 37 and 30-37 degrees c, respectively, and in the presence of 2-6 % (w/v) nacl. strains sw-145t and sw-156t were chemotaxonomically characterized as having ubiquinone-9 as the predominant respiratory lipoquinone and c(16 : 0), c(18 : 1)omega9c, c(16 : 1)omega9c and c(12 : 0) 3-oh as the major ... | 2004 | 15388746 |
| effects of hydrocarbon structure on fatty acid, fatty alcohol, and beta-hydroxy acid composition in the hydrocarbon-degrading bacterium marinobacter hydrocarbonoclasticus. | the lipids of the gram-negative bacterium marinobacter hydrocarbonoclasticus grown in a synthetic seawater medium supplemented with various hydrocarbons as the sole carbon source were isolated, purified, and their structures determined. the hydrocarbons were normal, iso, anteiso, and mid-chain branched alkanes, phenylalkanes, cyclohexylalkanes, and a terminal olefin. according to the sequential procedure used for lipid extraction, three pools were isolated: unbound lipids extracted with organic ... | 2004 | 15506246 |
| rapid screening and dereplication of bacterial isolates from marine sponges of the sula ridge by intact-cell-maldi-tof mass spectrometry (icm-ms). | rapid grouping of bacterial isolates is critical in comprehensive microbial studies of environmental samples or screening programmes e.g. in unknown marine environments where large numbers of strains have to be isolated on different growth media. sets of bacteria have been cultured from the marine sponges isops phlegraei, haliclona sp. 1, phakellia ventilabrum and plakortis sp. growing at a depth of about 300 m on the sula ridge close to the norwegian coast. we employed intact-cell maldi-tof (ic ... | 2004 | 15614563 |
| marinobacter bryozoorum sp. nov. and marinobacter sediminum sp. nov., novel bacteria from the marine environment. | two marine, gram-negative, aerobic, halophilic strains, designated kmm 3657t and kmm 3840t, were isolated and found to be phylogenetically closely related to each other, showing 96.6 % 16s rrna gene sequence similarity. both strains are members of the genus marinobacter in the gamma-proteobacteria (94.7-98.0 % 16s rrna gene sequence similarity). strain kmm 3657t and marinobacter lipolyticus sm19t were closely related, with 98.0 % sequence similarity. the novel strains shared generic physiologica ... | 2005 | 15653867 |
| characterization of a novel microperoxidase from marinobacter hydrocarbonoclasticus by electrospray ionization tandem mass spectrometry. | microperoxidases are small heme-peptides obtained by proteolytic digestion of cytochrome c, exhibiting peroxidase activity. they consist of a short- or medium-length polypeptide chain, covalently linked to an iron protoporphyrin ix moiety via two thioether bonds involving cys residues at the c-porphyrin a and b pyrrole rings. these small molecules are interesting for a wide range of possible applications. we have structurally characterized, by means of electrospray ionization (esi) mass and tand ... | 2005 | 15674861 |
| marinobacter hydrocarbonoclasticus gauthier et al. 1992 and marinobacter aquaeolei nguyen et al. 1999 are heterotypic synonyms. | the genus marinobacter was described with a single species, marinobacter hydrocarbonoclasticus gauthier et al. 1992, to place a hydrocarbon-degrading marine bacterium belonging to the gamma-subclass of the proteobacteria. marinobacter aquaeolei nguyen et al. 1999, the second described species of the genus, was proposed for a strain isolated from an oil-producing well on an offshore platform in southern vietnam, on the basis of differences in the whole-cell protein pattern and lipopolysaccharide ... | 2005 | 15879281 |
| biodegradation of cyclic nitramines by tropical marine sediment bacteria. | undersea deposition of unexploded ordnance (uxo) constitutes a potential source of contamination of marine environments by hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (hmx). the goal of the present study was to determine microbial degradation of rdx and hmx in a tropical marine sediment sampled from a coastal uxo field in the region of oahu island in hawaii. sediment mixed cultures growing in marine broth 2216 (21 degrees c) anaerobically mi ... | 2005 | 15915354 |
| a new microperoxidase from marinobacter hydrocarbonoclasticus. | the preparation and characterization of a new microperoxidase obtained from proteinase k-treated cytochrome c(552) from marinobacter hydrocarbonoclasticus (previously known as pseudomonas nautica) are presented. this microperoxidase (mmp-5) has novel structural properties relative to previously reported microperoxidases, as the two intervening amino acid (x) residues within the consensual cxxch c-type heme binding motif are missing, yielding a heme-pentapeptide with increased solubility in aqueo ... | 2005 | 15987663 |
| degradation of crude oil by an arctic microbial consortium. | the ability of a psychrotolerant microbial consortium to degrade crude oil at low temperatures was investigated. the enriched arctic microbial community was also tested for its ability to utilize various hydrocarbons, such as long-chain alkanes (n-c24 to n-c34), pristane, (methyl-)naphthalenes, and xylenes, as sole carbon and energy sources. except for o-xylene and methylnaphthalenes, all tested compounds were metabolized under conditions that are typical for contaminated marine liquid sites, na ... | 2005 | 15999222 |
| marinobacter maritimus sp. nov., a psychrotolerant strain isolated from sea water off the subantarctic kerguelen islands. | a psychrotolerant, gram-negative, motile bacterium, designated ck 47(t), was isolated from sea water off the subantarctic kerguelen islands (50 degrees 40' s 68 degrees 25' e). the isolate grew optimally at 22 degrees c and minimum and maximum temperature of growth were 4 and 37 degrees c, respectively. it required na(+) for growth and exhibited optimum growth at ph 8.5 and 4% nacl. it utilized hexane, heptane and petroleum ether as sole sources of carbon. strain ck 47(t) had q9 as the major res ... | 2005 | 16014465 |
| natural microbial diversity in superficial sediments of milazzo harbor (sicily) and community successions during microcosm enrichment with various hydrocarbons. | hydrocarbon-contaminated superficial sediments collected from the harbor of milazzo (tirrenean sea, northern sicily), a zone strongly affected by anthropogenic activities, were examined for in situ biodegradative capacities. a culture-independent molecular phylogenetic approach was used to study the influence of hydrocarbon and nutrient addition on the activity and diversity of the indigenous microbiota during a microcosm evaluation. the autochthonous microbial community in non-polluted sediment ... | 2005 | 16104865 |
| bacterial extracellular polymeric substance (eps): a carrier of heavy metals in the marine food-chain. | the ecological implications of metal binding properties of bacterial eps and its possible role in the bioaccumulation of pollutants in the marine food-chain was investigated using a partially purified and chemically characterized microbial eps isolated from a species of marinobacter. various factors influencing metal sorption by the eps including the influence of initial metal concentrations, incubation time, ph and sodium chloride concentrations on binding of lead (pb2+) and copper (cu2+) were ... | 2006 | 16256198 |
| use of electrospray ionization mass spectrometry for profiling of crude oil effects on the phospholipid molecular species of two marine bacteria. | we investigated the membrane lipid composition of two hydrocarbon-degrading gram-negative bacterial strains (pseudomonas nautica ip 617 and marinobacter hydrocarbonoclasticus) initially cultured on a soluble substrate, then on petroleum hydrocarbons, and finally taken back onto the soluble substrate. for the two strains, the growth on petroleum and the return to the initial medium showed major, but comparable, qualitative and quantitative modifications of the intact phospholipid molecular specie ... | 2005 | 16276494 |
| chemical defence in mussels: antifouling effect of crude extracts of the periostracum of the blue mussel mytilus edulis. | shells of the blue mussel mytilus edulis remain free of fouling organisms as long as they possess an intact periostracum, and a multiple antifouling defence that comprises a ripple-like microtopography and the production of chemical antifouling compounds has been suggested previously. this study investigates the chemical defence strategy of blue mussels for the first time. six crude extracts of the periostracum of intact shells were made using solvents of increasing polarity. these extracts were ... | 2006 | 17290869 |
| influence of crude oil on changes of bacterial communities in arctic sea-ice. | the danger of a petroleum hydrocarbon spillage in the polar, ice-covered regions is increasing due to oil exploration in arctic offshore areas and a growing interest in using the northern sea route (nsr) as an alternative transportation route for arctic oil and gas. however, little is known about the potential impact of accidental oil spills on this environment. we investigated the impact of crude oil on microbial community composition in six different arctic sea-ice samples incubated with crude ... | 2004 | 16329935 |
| marinobacter excellens sp. nov., isolated from sediments of the sea of japan. | five strains of halophilic, gram-negative marine bacteria (kmm 3809(t), kmm 3814, kmm 3815, kmm 3817 and kmm 3818) were isolated from sediments collected from chazhma bay, sea of japan. phylogenetic 16s rrna gene sequence-based analysis placed these bacteria in a clade within the genus marinobacter in the gamma-proteobacteria. kmm 3809(t) showed highest 16s rrna gene sequence similarity of 97.3 % to marinobacter litoralis and 96.9 % to marinobacter hydrocarbonoclasticus and marinobacter aquaeole ... | 2003 | 14657148 |
| biodegradation of benzene by halophilic and halotolerant bacteria under aerobic conditions. | a highly enriched halophilic culture was established with benzene as the sole carbon source by using a brine soil obtained from an oil production facility in oklahoma. the enrichment completely degraded benzene, toluene, ethylbenzene, and xylenes within 1 to 2 weeks. also, [14c]benzene was converted to 14co2, suggesting the culture's ability to mineralize benzene. community structure analysis revealed that marinobacter spp. were the dominant members of the enrichment. | 2004 | 14766609 |
| production of macroaggregates from dissolved exopolymeric substances (eps) of bacterial and diatom origin. | exopolymeric substances (eps) isolated from a pure culture of the marine bacterium marinobacter sp. and the marine diatom skeletonema costatum (axenic) were partially purified, chemically characterized and used as dissolved organic matter (dom) for the production of macroaggregates. the role of organic particles such as transparent exopolymeric particles (tep) and coomassie stained particles (csp) in the production of macroaggregates was experimentally assessed. three experimental rolling tanks ... | 2005 | 16329945 |
| the effect of dissolved organic carbon on bacterial adhesion to conditioning films adsorbed on glass from natural seawater collected during different seasons. | adhesion of three marine bacterial strains, i.e. marinobacter hydrocarbonoclasticus, psychrobacter sp. and halomonas pacifica with different cell surface hydrophobicities was measured on glass in a stagnation point flow chamber. prior to bacterial adhesion, the glass surface was conditioned for 1 h with natural seawater collected at different seasons in order to determine the effect of seawater composition on the conditioning film and bacterial adhesion to it. the presence of a conditioning film ... | 2003 | 14768468 |
| isolation and characterization of novel hydrocarbon-degrading euryhaline consortia from crude oil and mangrove sediments. | two novel and versatile bacterial consortia were developed for the biodegradation of hydrocarbons. they were isolated from crude oil from the cormorant field in the north sea (mpd-7) and from sediment associated with mangrove roots (mpd-m). the bacterial consortia were able to degrade both aliphatic and aromatic hydrocarbons in crude oils very effectively in seawater (35 g/l nacl) and synthetic media containing 0 to 100 g/l nacl (1.7 m). salinities over twice that of normal seawater decreased th ... | 2000 | 14961176 |
| incorporation of 1-chlorooctadecane into fa and beta-hydroxy acids of marinobacter hydrocarbonoclasticus. | the lipids of the gram-negative marine bacterium marinobacter hydrocarbonoclasticus, cultivated in synthetic seawater supplemented with 1-chlorooctadecane as sole source of carbon, were isolated, purified, and their structures determined. three pools of lipids were isolated according to the sequential procedure used: unbound lipids extracted by organic solvents, ester-bound lipids released under alkaline conditions, and amide-bound lipids released by acid hydrolysis. fa isolated from the unbound ... | 2004 | 15055238 |
| total synthesis of petrobactin and its homologues as potential growth stimuli for marinobacter hydrocarbonoclasticus, an oil-degrading bacteria. | a modular synthesis was developed to access petrobactin, a catechol-containing siderophore isolated from marinobacter hydrocarbonoclasticus. a range of petrobactin homologues with differing dihydroxybenzamide motifs and in one case an increased number of carbons in the polyamine backbone were also synthesized. as such, these systems represent new isomeric probes to study iron transport properties in m. hydrocarbonoclasticus. the synthesis of petrobactin and its homologues and the first biologica ... | 2004 | 15132566 |
| [lipid composition of novel shewanella species isolated from far eastern seas]. | a comparative study of the lipid composition of 26 strains (including type strains) of marine gammaproteobacteria belonging to the genera shewanella, alteromonas, pseudoalteromonas, marinobacterium, microbulbifer, and marinobacter was carried out. the bacteria exhibited genus-specific profiles of ubiquinones, phospholipids, and fatty acids, which can serve as reliable chemotaxonomic markers for tentative identification of new isolates. the studied species of the genus shewanella were distinguish ... | 2005 | 16400986 |
| marine amphiphilic siderophores: marinobactin structure, uptake, and microbial partitioning. | marinobactins a-e are a suite of amphiphilic siderophores which have a common peptidic head group that coordinates fe(iii), and a fatty acid which varies in length and saturation. as a result of the amphiphilic properties of these siderophores it is difficult to study siderophore-mediated uptake of iron, because the amphiphilic siderophores partition indiscriminately in microbial and other membranes. an alternative method to distinguish amphiphilic siderophore partitioning versus siderophore-med ... | 2007 | 17868890 |
| fatty acid and hydroxy acid adaptation in three gram-negative hydrocarbon-degrading bacteria in relation to carbon source. | the lipids of three gram-negative bacteria, acinetobacter calcoaceticus, marinobacter aquaeolei, and pseudomonas oleovorans grown on mineral media supplemented with ammonium acetate or hydrocarbons, were isolated, purified, and their structures determined. three pools of lipids were isolated according to a sequential procedure: unbound lipids extracted with organic solvents, comprising metabolic lipids and the main part of membrane lipids, oh--labile lipids (mainly ester-bound in the lipopolysac ... | 2005 | 16477811 |
| marinobacter algicola sp. nov., isolated from laboratory cultures of paralytic shellfish toxin-producing dinoflagellates. | phylogenetic and phenotypic analysis of cultivable marine bacteria isolated from laboratory cultures of two paralytic shellfish toxin-producing dinoflagellates, gymnodinium catenatum and alexandrium tamarense, showed the presence of a novel group of gram-negative, aerobic, moderately halophilic and hydrocarbon-degrading bacteria, related to the genus marinobacter. the strains, designated dg893t, dg1136 and atam407-13, grew optimally in media with 3-6 % nacl and at 25-30 degrees c, and all could ... | 2006 | 16514021 |
| molecular microbial diversity of a soil sample and detection of ammonia oxidizers from cape evans, mcmurdo dry valley, antarctica. | the aim of our study was to estimate the uncultured eubacterial diversity of a soil sample collected below a dead seal, cape evans, mcmurdo, antarctica by an ssu rdna gene library approach. our study by sequencing of clones from ssu rdna gene library approach revealed high diversity in the soil sample from antarctica. more than 50% of clones showed homology to cytophaga-flavobacterium-bacteroides group; sequences also belonged to alpha, beta, gamma proteobacteria, thermus-deinococcus and high gc ... | 2007 | 16517136 |
| atp level variations in heterotrophic bacteria during attachment on hydrophilic and hydrophobic surfaces. | a survey of the extracellular atp levels of 86 heterotrophic bacteria showed that gram-negative bacteria of the genera sulfitobacter, staleya, and marinobacter secreted elevated amounts of extracellular atp, ranging from 6.0 to 9.8 pm atp/colony forming unit (cfu), and that gram-positive bacteria of the genera kocuria and planococcus secreted up to 4.1 pm atp/cfu. variations in the levels of extracellular and intracellular atp-dependent luminescence were monitored in living cells of sulfitobacte ... | 2006 | 16636988 |
| [phylogenetic diversity and cold-adaptive hydrolytic enzymes of culturable psychrophilic bacteria associated with sea ice from high latitude ocean, artic]. | the phylogenetic diversity of culturable psychrophilic bacteria associated with sea ice from high latitude sea (77 degrees 30'n - 81 degrees 12'n), canadian basin and greenland sea arctic, was investigated. a total of 37 psychrophilic strains were isolated using three different methods of ( i ) spread plate method: 100 microl of each dilution ice-melt sample was spreaded onto the surface of marine 2216 agar (difco laboratories, detroit, mi) and incubated for 2 to 6 weeks at 4 degrees c; ( ii ) b ... | 2006 | 16736573 |
| characterization of hydrocarbonoclastic bacterial communities from mangrove sediments in guanabara bay, brazil. | hydrocarbonoclastic bacterial communities inhabiting mangrove sediments were characterized by combining molecular and culture-dependent approaches. surface sediments were collected at two sampling sites in guanabara bay (rio de janeiro, brazil) and used to inoculate in vitro enrichment cultures containing crude oil to obtain hydrocarbonoclastic bacterial consortia. in parallel, in situ mesocosms (located in the guapimirim mangrove) were contaminated with petroleum. comparison of bacterial commun ... | 2006 | 16815684 |
| endozoicomonas elysicola gen. nov., sp. nov., a gamma-proteobacterium isolated from the sea slug elysia ornata. | a gram-negative, strictly aerobic, rod-formed bacterium, strain mkt110(t), was isolated from a mollusk, the sea slug elysia ornata collected in seawater off the coast of izu-miyake island, japan at a depth of 15m. comparative 16s rrna gene sequences analysis indicated that the isolate mkt110(t) constituted a novel lineage in gamma-proteobacteria related to the genera zooshikella, oceanospirillum, microbulbifer, marinobacter, saccharospirillum and pseudomonas. the strain mkt110(t) was closely rel ... | 2007 | 16904280 |
| phylogenetic analysis of a biofilm bacterial population in a water pipeline in the gulf of mexico. | the aim of this study was to assess the bacterial diversity associated with a corrosive biofilm in a steel pipeline from the gulf of mexico used to inject marine water into the oil reservoir. several aerobic and heterotrophic bacteria were isolated and identified by 16s rrna gene sequence analysis. metagenomic dna was also extracted to perform a denaturing gradient gel electrophoresis analysis of ribosomal genes and to construct a 16s rrna gene metagenomic library. denaturing gradient gel electr ... | 2006 | 16958915 |
| phylogenetic diversity and activity of aerobic heterotrophic bacteria from a hypersaline oil-polluted microbial mat. | the diversity and function of aerobic heterotrophic bacteria (ahb) in cyanobacterial mats have been largely overlooked. we used culture-dependent and molecular techniques to explore the species diversity, degradative capacities and functional guilds of ahb in the photic layer (2mm) of an oil-polluted microbial mat from saudi arabia. enrichment isolation was carried out at different salinities (5% and 12%) and temperatures (28 and 45 degrees c) and on various substrates (acetate, glycolate, spiru ... | 2007 | 17056222 |
| analysis of the composition of bacterial communities in oil reservoirs from a southern offshore brazilian basin. | the aim of this study was to characterize and compare the bacterial community structure of two distinct oil samples from a petroleum field in brazil by using both molecular, based on the construction of 16s rrna gene libraries, and cultivation methods. statistical comparisons of libraries based on amplified ribosomal dna restriction analysis (ardra) data revealed no significant differences between the communities recovered in the non-biodegraded (nbd) and highly biodegraded oils (hbd). blastn an ... | 2007 | 17072536 |
| marinobacter vinifirmus sp. nov., a moderately halophilic bacterium isolated from a wine-barrel-decalcification wastewater. | a halophilic, gram-negative, motile, non-sporulating bacterium designated strain fb1t was isolated from a wine-barrel-decalcification wastewater. the organism comprises straight rods and has a strictly respiratory metabolism with o2. strain fb1t grows optimally at 20-30 degrees c and 5-6% nacl. the predominant fatty acids were found to be c18:1omega9c (30.4%), c16:0 (25.7%), c12:0 3-oh (10.3%), c16:1omega9c (9.7%) and c16:1omega7c (8.4%). a phylogenetic analysis based on 16s rrna gene sequences ... | 2006 | 17082382 |
| marinobacter koreensis sp. nov., isolated from sea sand in korea. | a marine, gram-negative, aerobic, motile, straight-rod-shaped, moderately halophilic bacterium, designated strain dd-m3t, was isolated from sea sand in pohang, korea. a phylogenetic tree based on 16s rrna gene sequences showed that the strain fell within the evolutionary radiation encompassed by the genus marinobacter. the levels of 16s rrna gene sequence similarity between the novel strain and the type strains of recognized marinobacter species ranged from 94.2 to 97.6%, the highest values bein ... | 2006 | 17082406 |
| analysis of nitrite reductase (nirk and nirs) genes and cultivation reveal depauperate community of denitrifying bacteria in the black sea suboxic zone. | chemical profiles of the black sea suboxic zone show a distribution of nitrogen species which is traditionally associated with denitrification, i.e. a secondary nitrite maximum associated with nitrate depletion and a n(2) gas peak. to better understand the distribution and diversity of the denitrifier community in the black sea suboxic zone, we combined a cultivation approach with cloning and sequencing of pcr-amplified nitrite reductase (nirs and nirk) genes. the black sea suboxic zone appears ... | 2007 | 17227417 |
| [characterization of bacterial diversity in the shengli-s12 oil reservoir by culture-dependent and culture-independent methods]. | examining bacterial diversity in an oil reservoir of shengli oil field by both culture-independent molecular technique and enrichment method. | 2008 | 18956759 |
| optical biosensor for the determination of bod in seawater. | an automatic sensing system was developed using an optical bod sensing film. the sensing film consists of an organically modified silicate (ormosil) film embedded with an oxygen-sensitive ru complex. a multi-microorganisms immobilization method was developed for the bod sensing film preparation. three different kinds of microorganisms, bacillus licheniformis, dietzia maris and marinobacter marinus from seawater, were immobilized on a polyvinyl alcohol ormosils. after preconditioning, the bod bio ... | 2006 | 18970735 |
| marinobacter gudaonensis sp. nov., isolated from an oil-polluted saline soil in a chinese oilfield. | two novel strains, sl014b61a(t) and sl014b11a, were isolated from an oil-polluted saline soil from gudao in the coastal shengli oilfield, eastern china. cells of strains sl014b61a(t) and sl014b11a were motile, gram-negative and rod-shaped. growth occurred at nacl concentrations of between 0 and 15 % and at temperatures of between 10 and 45 degrees c. strain sl014b61a(t) had q9 as the major respiratory quinone and c16 : 0 (21.2 %), c18 : 1omega9c (20.3 %), c16 : 1omega7c (7.3 %) and c16 : 1omega9 ... | 2007 | 17267959 |
| biosynthesis of isoprenoid wax ester in marinobacter hydrocarbonoclasticus dsm 8798: identification and characterization of isoprenoid coenzyme a synthetase and wax ester synthases. | marinobacter hydrocarbonoclasticus dsm 8798 has been reported to synthesize isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source under limiting nitrogen and/or phosphorous conditions. we hypothesized that isoprenoid wax ester synthesis involves (i) activation of an isoprenoid fatty acid by a coenzyme a (coa) synthetase and (ii) ester bond formation between an isoprenoid alcohol and isoprenoyl-coa catalyzed, most likely, by an isoprenoid wax ester synthase similar ... | 2007 | 17351040 |
| petrobactin is the primary siderophore synthesized by bacillus anthracis str. sterne under conditions of iron starvation. | the siderophores of bacillus anthracis are critical for the pathogen's proliferation and may be necessary for its virulence. bacillus anthracis str. sterne cells were cultured in iron free media and the siderophores produced were isolated and purified using a combination of xad-2 resin, reverse-phase fplc, and size exclusion chromatography. a combination of 1h and 13c nmr spectroscopy, uv spectroscopy and esi-ms/ms fragmentation were used to identify the primary siderophore as petrobactin, a cat ... | 2005 | 16388397 |
| [phylogenetic diversity of culturable bacteria in the ancient salt deposits of the yipinglang salt mine, p. r. china]. | the microbial diversity of cultivable bacteria, isolated from the ancient salt deposits from the yipinglang salt mine (ypl) in the yunnan province, p. r. china,was investigated by using conventional culture-dependent method and phylogenetic analyses based on 16s rrna gene sequence comparisons. 38 bacteria strains were isolated from the brine, halite and saline soil samples on mba (marine broth agar 2216, difco) and isp 2 (international streotomyces project medium 2) media supplemented with 0.5-3 ... | 2007 | 17944352 |
| cytoplasmic wax ester accumulation during biofilm-driven substrate assimilation at the alkane--water interface by marinobacter hydrocarbonoclasticus sp17. | during growth on n-alkanes, the marine bacterium marinobacter hydrocarbonoclasticus sp17 formed a biofilm at the alkane-water interface. we showed that hexadecane degradation was correlated with biofilm development and that alkane uptake is localized in the biofilm but not in the bulk medium. biofilms were observed in cultures on metabolizable n-alkanes (c8-c28) and n-alcohols (c12 and c16), but were formed neither on non-metabolizable alkanes (pristane, heptamethylnonane and n-c32) nor on inert ... | 2008 | 18191384 |
| vesicles to concentrate iron in low-iron media: an attempt to mimic marine siderophores. | amphiphilic catechol-type iron chelators were studied with the aim of mimicking the properties of marine bacterial siderophores. the fe(iii) complexation constants and aqueous solution speciation of l(s10), a sulfonated catechol unit that has a c(10) lipophilic carbon chain connected by an amide linkage, were determined by spectrophotometric titration. the calculated value of pfe3+ is 18.1 at ph 7.4. cryogenic transmission electron microscopy showed that the tris(catecholate) ferric complex form ... | 2008 | 18293349 |
| biochemical characterization of atud from pseudomonas aeruginosa, the first member of a new subgroup of acyl-coa dehydrogenases with specificity for citronellyl-coa. | the aturabcdefgh gene cluster is essential for acyclic terpene utilization (atu) in pseudomonas aeruginosa. the biochemical functions of most atu proteins have not been experimentally verified; exceptions are atuc/atuf, which constitute the two subunits of geranyl-coa carboxylase, the key enzyme of the atu pathway. in this study we investigated the biochemical function of atud and of the pa1535 gene product, a protein related to atud in amino acid sequence. 2d gel electrophoresis showed that atu ... | 2008 | 18310025 |
| spongiibacter marinus gen. nov., sp. nov., a halophilic marine bacterium isolated from the boreal sponge haliclona sp. 1. | strain hal40b(t) was isolated from the marine sponge haliclona sp. 1 collected at the sula ridge off the norwegian coast and characterized by physiological, biochemical and phylogenetic analyses. the isolate was a small rod with a polar flagellum. it was aerobic, gram-negative and oxidase- and catalase-positive. optimal growth was observed at 20-30 degrees c, ph 7-9 and in 3 % nacl. substrate utilization tests were positive for arabinose, tween 40 and tween 80. enzyme tests were positive for alk ... | 2008 | 18319460 |
| biodiversity of polycyclic aromatic hydrocarbon-degrading bacteria from deep sea sediments of the middle atlantic ridge. | the bacteria involved in the biodegradation of polycyclic aromatic hydrocarbons (pahs) in deep sea subsurface environments are largely unknown. in order to reveal their biodiversity, sediments from 2.2 m under the bottom surface at a water depth of 3542 m were sampled on the middle atlantic ridge with a gravity column sampler. the sediments were promptly enriched with either crude oil or a mixture of pahs (naphthalene, phenanthrene and pyrene) as the sole carbon source, and further enriched with ... | 2008 | 18445026 |
| marinobacter psychrophilus sp. nov., a psychrophilic bacterium isolated from the arctic. | strain 20041(t) was isolated from sea-ice of the canadian basin (7 degrees 23' 14'' n 14 degrees 06' 55'' w). phylogenetic analysis based on 16s rrna gene homology showed that strain 20041(t) was related to members of the genus marinobacter and had highest 16s rrna gene sequence similarity with the type strain of marinobacter maritimus. cells were gram-negative, rod-shaped, psychrophilic and motile. the temperature range for growth was 0-22 degrees c, with optimum growth occurring at 16-18 degre ... | 2008 | 18523195 |
| marinobacter salsuginis sp. nov., isolated from the brine-seawater interface of the shaban deep, red sea. | two moderately halophilic gram-negative bacteria were isolated from a sample taken from the brine-seawater interface of the shaban deep in the red sea. phylogenetic analysis of the 16s rrna gene sequence showed that these organisms represent a novel species of the genus marinobacter. cells of the new isolates formed non-pigmented colonies and were motile by means of a single polar flagellum. strains sd-14b(t) and sd-14c grew optimally at 35-37 degrees c, in 5 % nacl and at ph 7.5-8.0. the organi ... | 2007 | 17473254 |
| obligate oil-degrading marine bacteria. | over the past few years, a new and ecophysiologically unusual group of marine hydrocarbon-degrading bacteria - the obligate hydrocarbonoclastic bacteria (ohcb) - has been recognized and shown to play a significant role in the biological removal of petroleum hydrocarbons from polluted marine waters. the introduction of oil or oil constituents into seawater leads to successive blooms of a relatively limited number of indigenous marine bacterial genera--alcanivorax, marinobacter, thallassolituus, c ... | 2007 | 17493798 |
| [microbial diversity in shengli petroleum reservoirs analyzed by t-rflp]. | recent investigations on the microbial ecology of oil reservoirs in a variety of locales indicated that these habitats harbor various assemblages. in this study, a cultured-independent molecular technique, terminal restriction fragment length polymorphism (t-rflp), was used to analyze the microbial diversity of an injection well (s12-zhu) and three related production wells (s12-4, s12-5 and s12-19) in the shengli oilfield (shandong province, china). the 16s rrna genes were amplified by pcr with ... | 2007 | 17552237 |
| fluctuation of microbial activities after influent load variations in a full-scale sbr: recovery of the biomass after starvation. | due to variations in the production levels, a full-scale sequencing batch reactor (sbr) for post-treatment of tannery wastewater was exposed to low and high ammonia load periods. in order to study how these changes affected the n-removal capacity, the microbiology of the reactor was studied by a diverse set of techniques including molecular tools, activity tests, and microbial counts in samples taken along 3 years. the recover capacity of the biomass was also studied in a lab-scale reactor opera ... | 2009 | 19629470 |
| metal-dependent self-assembly of a microbial surfactant. | small-angle neutron scattering (sans), cryogenic transmission electron microscopy (cryo-tem), and dynamic light scattering (dls) were used to study the metal-dependent phase behavior of microbially produced surfactants-marinobactins b, d, and e (mb, md, and me). marinobactins a-e are siderophores that facilitate fe(iii) acquisition by the source bacterium through the coordination of fe(iii) by the peptidic headgroup. all of the marinobactins have the same six amino acid headgroup but differ in t ... | 2007 | 17655261 |
| alkaliphilic and halophilic hydrocarbon-utilizing bacteria from kuwaiti coasts of the arabian gulf. | green animate materials from the intertidal zone of the arabian gulf coast accommodated more alkaliphilic and halophilic bacteria than inanimate materials. the alkaliphilic oil-utilizing bacteria, as identified by their 16s ribonucleic acid sequences, belonged to the following genera arranged in decreasing frequences: marinobacter, micrococcus, dietzia, bacillus, oceanobacillus, and citricoccus. the halophilic oil-utilizing bacteria belonged to the genera: marinobacter, georgenia, microbacterium ... | 2007 | 17710391 |
| marinobacter segnicrescens sp. nov., a moderate halophile isolated from benthic sediment of the south china sea. | a gram-negative, motile, non-spore-forming and moderately halophilic ellipsoid-shaped marine coccobacillus, designated strain ss011b1-4(t), was isolated from benthic sediment of the south china sea. optimum growth occurred at 30-37 degrees c, ph 7.5-8.0 and 4-8 % (w/v) nacl. strain ss011b1-4(t) utilized a variety of organic substrates as sole carbon sources, but did not utilize toluene, n-tetradecane or crude oil. strain ss011b1-4(t) had ubiquinone-9 as the major respiratory quinone and c(18 : 1 ... | 2007 | 17766857 |
| marinobacter salicampi sp. nov., isolated from a marine solar saltern in korea. | a gram-negative, motile, rod-shaped, marinobacter-like bacterial strain, isl-40(t), was isolated from a marine solar saltern of the yellow sea in korea. the taxonomic position of the novel strain was investigated using a polyphasic approach. strain isl-40(t) grew optimally at ph 7.0-8.0 and at 30 degrees c. it contained q-9 as the predominant ubiquinone. the major fatty acids were c(16 : 0), c(16 : 1)omega7c and/or iso-c(15 : 0) 2-oh and 10-methyl c(16 : 0). the dna g+c content was 58.1 mol%. a ... | 2007 | 17766879 |
| borate binding to siderophores: structure and stability. | well-known as specific iron chelating agents produced by bacteria, it is shown that some, but not all, siderophore classes have an unexpected binding affinity for boron. the relevant criterium is the availability of a vicinal dianionic oxygen containing binding group (i.e., citrate or catecholate). the resulting boron complexes have been characterized by esi-ms, multinuclear nmr, and dft calculations. detailed boron binding constants have been measured for vibrioferrin, rhizoferrin, and petrobac ... | 2007 | 17850151 |
| purification, characterization, and potential bacterial wax production role of an nadph-dependent fatty aldehyde reductase from marinobacter aquaeolei vt8. | wax esters, ester-linked fatty acids and long-chain alcohols, are important energy storage compounds in select bacteria. the synthesis of wax esters from fatty acids is proposed to require the action of a four-enzyme pathway. an essential step in the pathway is the reduction of a fatty aldehyde to the corresponding fatty alcohol, although the enzyme responsible for catalyzing this reaction has yet to be identified in bacteria. we report here the purification and characterization of an enzyme fro ... | 2009 | 19270127 |