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phylogenetic and chemotaxonomic characterization of acidaminococcus fermentans.the phylogenetic position of acidaminococcus fermentans was determined by comparative sequence analysis of the 16s rrna. this gram-negative bacterium is a member of the sporomusa cluster that is defined by other gram-negative bacteria, i.e. sporomusa, megasphaera, selenomonas, butyrivibrio, pectinatus, and zymophilus. the branching point of this group within the radiation of gram-positive bacteria of the clostridium/bacillus subphylum and adjacent to peptococcus niger could be confirmed. chemota ...19921385264
susceptibilities of anaerobic bacteria isolated from animals with ovine foot rot to 28 antimicrobial agents.the agar dilution method was used to determine the inhibitory activities of 28 antimicrobial agents against 35 strains of the genus peptostreptococcus, 4 strains of the species peptococcus niger, 20 strains of the species megasphaera elsdenii, 7 strains from the species acidaminococcus fermentans, 8 strains of the genus clostridium, 11 strains of the genus eubacterium, and 1 strain of the species propionibacterium acidipropionici, all of which were isolated from 125 clinical cases of ovine foot ...19921590689
the phylogenetic position of peptococcus niger based on 16s rrna sequence studies.a 1330 base-pair fragment of a 16s rrna gene has been amplified, cloned and sequenced. comparison to other 16s rrna sequences of eubacteria showed that p. niger represents a deep branch within the subdivision "gram-positive with gram-negative cell walls". it is not related to peptostreptococci, representatives of this genus studied so far are more closely related to clostridia.19901703504
isolation and identification of intestinal steroid-desulfating bacteria from rats and humans.we isolated 12 strictly anaerobic steroid-3-sulfate-desulfating strains from the intestinal floras of rats and humans. two strains (s1 and s2) of the same atypical clostridium species and an atypical lactobacillus strain (termed r9) were obtained from rats. the human isolates were identified as eubacterium cylindroides (two strains, h1 and h2), peptococcus niger (two strains, h4 and h89), and clostridium clostridiiforme. we also isolated, from different human fecal samples, four strains of pheno ...19883178214
a numerical taxonomic study of the gram-positive anaerobic cocci.clinical (101) and collection (26) strains of gram-positive anaerobic cocci were examined in conventional tests and pyrolysis mass spectrometry (pms). numerical classifications based upon conventional test reaction patterns (ctrps) and pms showed 27 and 22 clusters, respectively. cross-tabulation of cluster membership in the two classifications showed excellent correlation, with the combined classifications showing clear groups corresponding to the currently recognised species peptostreptococcus ...19957629855
rapid diagnosis of anaerobic gram-positive cocci by salt tolerance.the maximum salt tolerance was 2.5% in cases of peptostreptococcus anaerobius and peptococcus prevotii, 3% in ruminococcus albus, 4% in acidaminococcus fermentans, peptococcus niger and megasphaera elsdenii and 5% in peptococcus magnus. the biochemical tests being largely inactive and time consuming the findings of salt tolerance if combined with those of morphological data provide clue to the rapid identification of gram-positive anaerobic cocci.19947963612
anaerobic flora of the conjunctival sac in patients with aids and with anophthalmia compared with normal eyes.relatively few investigations of anaerobic bacteria as ocular flora have been conducted, and their results have been contradictory. the conjunctival sacs of 22 normal subjects and of 14 patients with acquired immunodeficiency syndrome, and 22 anophthalmic sockets were cultured for anaerobic bacteria thirty-four (77.3%) of the 44 eyes of normal subjects harbored anaerobic bacteria; propionibacterium acnes was present in 28 eyes (63.6%), lactobacillus species in 6 eyes (13.6%), and veillonella spe ...19948079632
16s ribosomal dna sequences of anaerobic cocci and proposal of ruminococcus hansenii comb. nov. and ruminococcus productus comb. nov.the 16s ribosomal dna sequences of representative members of the family peptococcaceae were determined. the members of the family examined were divided into the following four phylogenetic groups: peptococcus niger atcc 27731t (t = type strain), the sarcina-peptostreptococcus anaerobius group, the ruminococcus-coprococcus group, and the peptostreptococcus group. peptococcus niger, the type species of the family, was not related to other members of the family. peptostreptococcus anaerobius atcc 2 ...19948123556
non-sporing anaerobes in certain surgical group of patients.the magnitude of non-sparing anaerobic (nsa) infections has been defined in the postoperative wounds on colorectum in children (57.1%), general surgery (0%), abdominoperineal and uterocervical operations (11-45%) in gynaecologic and obstetrical cases and perforative peritonitis (25.8-32.3%). children below the 6 months age group bear less risk of acquiring nsa infection. under certain situations, metronidazole combats nsa infections in a better way than other antibacterials. the bacteriology of ...19958648152
the in vitro activity of trospectomycin against anaerobic bacteria.the authors evaluated the activity of trospectomycin, a new aminocyclitol which is characterized by good antibacterial and broad spectrum activity, in comparison with clindamycin and ampicillin on a sample of recent isolates: bacteroides fragilis (15 strains), bacteroides urealyticus (5 strains), bacteroides vulgatus (5 strains), bacteroides spp. (15 strains), prevotella melaninogenica (6 strains), porphyromonas asaccharolytica (7 strains), mobiluncus spp. (3 strains), peptococcus niger (3 strai ...19958667035
effects of laparoscopic lavage on adhesion formation and peritoneum in an animal model of pelvic inflammatory disease.to develop an animal model of pelvic inflammatory disease (pid) that simulates intraluminal tubal damage and adhesion formation seen in human pid, and to evaluate peritoneal effects of laparoscopic lavage with 1% povidone-iodine, 0.5% povidone-iodine, and 0.05% chlorhexidine gluconate solutions.200212386364
[research on the pathogenicity and pigmentation of micrococcus niger]. 195913854402
micrococcus niger, a new pigment-forming anaerobic coccus recovered from urine in a case of general arteriosclerosis. 193016559465
rapid identification of non-sporing anaerobes using nuclear magnetic resonance spectroscopy and an identification strategy.the non-sporing anaerobes cause a wide spectrum of infections. they are difficult to culture and their identification is tedious and time-consuming. rapid identification of anaerobes is highly desirable. towards this end, the potential of nuclear magnetic resonance (nmr) spectroscopy for providing a fingerprint within the proton spectrum of six genera belonging to anaerobes reflecting their characteristic metabolites has been investigated.200718087080
[identification of anaerobic bacteria isolated from various clinical specimens and determination of antibiotic susceptibilities].routine isolation, identification and susceptibility testing of anaerobic bacteria present several difficulties leading to defects in the determination of local susceptibility patterns which will guide empirical treatment protocols. this study was carried out to identify the anaerobic bacteria isolated from various clinical materials obtained from the suspected patients with anaerobic infection and to determine the antibiotic susceptibilities against several antibiotics. one hundred clinical spe ...201020549955
analysis of the bacterial diversity existing on animal hide and wool: development of a preliminary pcr-restriction fragment length polymorphism fingerprint database for identifying isolates.twenty-one bacterial strains were isolated from imported cattle hide and rabbit wool using two types of media, nutrient broth, and nutrient broth with serum. the bacteria identified were brevibacillus laterosporus, leclercia adecarboxylata, peptococcus niger, bacillus circulans, raoultella ornithinolytica, bacillus subtilis, bacillus cereus, bacillus thermobacillus, bacillus choshinensis, bacillus sphaericus, acinetobacter haemolyticus, sphingomonas paucimobilis, bacillus thuringiensis, staphylo ...201223451394
steroid sulfatase activity in a peptococcus niger strain from the human intestinal microflora.a strictly anaerobic gram-positive coccus, identified as peptococcus niger, that developed sulfatase activity towards steroid-3-sulfate esters was isolated from human fecal material. this strain desulfated the arylsulfate esters estrone-3-sulfate (100%) and beta-estradiol-3-sulfate (50%); only trace amounts of desulfated estriol-3-sulfate were found. in addition, alkylsulfatase activity was found towards the 3 alpha-sulfates of 5 alpha-androstane-17-one and 5 beta-androstane-17-one and towards t ...19873477998
influence of intestinal bacterial desulfation on the enterohepatic circulation of dehydroepiandrosterone sulfate.selective association of germ-free (gf) rats with dehydroepiandrosterone sulfate (dheas) desulfating bacteria allowed us to assess the exact impact of intestinal bacterial desulfation on the excretion and enterohepatic circulation of orally administered dheas. germ-free rats selectively associated with the dheas-desulfating strain peptococcus niger h4 (h4 rats) excreted 50% of the total label recovered within 17 h vs 21 h in gf rats and 13 h 23 min in conventional (cv) rats. germ-free rats excre ...19902145475
partial characterization of the steroidsulfatases in peptococcus niger h4.the strictly anaerobic intestinal peptococcus niger h4 synthesizes three different steroidsulfatase enzymes: a constitutive arylsulfatase and two inducible alkylsteroidsulfatases. the arylsulfatase desulfates estrogen-3-sulfates and phenylsulfates. the two alkylsteroidsulfatases desulfate, respectively, 3 alpha-sulfates and 3 beta-sulfates of delta 5, 5 alpha, and 5 beta androstanes, pregnanes, and bile acids. cholesterol-3 beta-sulfate was not desulfated by the alkylsteroidsulfatases nor were s ...19912036022
the laboratory identification of gram-positive anaerobic cocci.a collection of 256 clinical strains and 40 reference strains of gram-positive anaerobic cocci (gpac) was studied, to characterise the recognised species more fully and to define groups of strains which might correspond to previously undescribed species. the methods used were: gas-liquid chromatography (glc) for the detection of volatile fatty acids (vfas); determination of the pre-formed enzyme profile with a commercially available kit, atb 32a; microscopic appearance; colonial morphology; and ...19912030504
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