| copper-resistant bacteria from industrial effluents and their role in remediation of heavy metals in wastewater. | six copper-resistant bacterial strains were isolated from wastewater of tanneries of kasur and rohi nala. two strains tolerated copper at 380 mg/l, four up to 400 mg/l. three strains were identified as members of the genus salmonella; one strain was identified as streptococcus pyrogenes, one as vagococcus fluvialis and the last was identified as escherichia coli. the ph and temperature optimum for two of them were 7.0 and 30 degrees c, respectively; four strains had corresponding optima at 7.5 a ... | 2002 | 11980269 |
| microbial biodiversity in cheese consortia and comparative listeria growth on surfaces of uncooked pressed cheeses. | the study set out to determine how changes in the microbial diversity of a complex antilisterial consortium from the surface of st-nectaire cheese modify its antilisterial activities. on the basis of the microbial composition of a natural complex consortium named tr15 (truefood consortium 15), three new consortia of different species and strain compositions were defined: tr15-sc (58 isolates from tr15 collection), tr15-m (pools of isolates from selective counting media) and tr15-bhi (pools of is ... | 2014 | 24463156 |
| identification of enterococcus species and phenotypically similar lactococcus and vagococcus species by reverse checkerboard hybridization to chaperonin 60 gene sequences. | data from four recent studies (s. h. goh et al., j. clin. microbiol. 36:2164-2166, 1998; s. h. goh et al., j. clin. microbiol. 34:818-823, 1996; s. h. goh et al., j. clin. microbiol. 35:3116-3121, 1997; a. y. c. kwok et al., int. j. syst. bacteriol. 49:1181-1192, 1999) suggest that an approximately 600-bp region of the chaperonin 60 (cpn60) gene, amplified by pcr with a single pair of degenerate primers, has utility as a potentially universal target for bacterial identification (id). this cpn60 ... | 2000 | 11060051 |
| vagococcus fessus sp. nov., isolated from a seal and a harbour porpoise. | a polyphasic taxonomic study was performed on two strains of an unknown gram-positive, catalase-negative, coccus-shaped bacterium isolated from a dead seal and a harbour porpoise. comparative 16s rrna gene sequencing demonstrated that the unknown bacterium represents a new subline within the genus vagococcus close to, but distinct from, vagococcus fluvialis, vagococcus lutrae and vagococcus salmoninarum. the unknown bacterium was readily distinguished from the three currently recognized vagococc ... | 2000 | 10843057 |
| use of tests for acidification of methyl-alpha-d-glucopyranoside and susceptibility to efrotomycin for differentiation of strains of enterococcus and some related genera. | a total of 107 enterococcus strains, 10 vagococcus fluvialis strains, and 8 lactococcus garvieae strains were tested for acidification of methyl-alpha-d-glucopyranoside (mgp) and susceptibility to 100-microg efrotomycin (efro) disks. all 26 strains of enterococcus casseliflavus, including 3 nonmotile and 2 nonpigmented strains, acidified mgp and were resistant to efro. all 22 strains of enterococcus gallinarum, including 5 nonmotile strains, also acidified mgp and were resistant to efro. none of ... | 1998 | 9620381 |
| d-alanylcardiolipin, a major component of the unique lipid pattern of vagococcus fluvialis. | motile group n streptococci, classified as vagococcus fluvialis, have been isolated from cows' udders, human and animal feces, river water, and seawater. they possess an unusual membrane lipid and fatty acid pattern. we isolated and characterized 13 polar lipids, 8 of them also found in other gram-positive bacteria: mono- and dihexosyldiacylglycerol, an acylated and a glycerophosphate-substituted derivative of the latter, cardiolipin, phosphatidylglycerol, d-alanylphosphatidylglycerol, and l-lys ... | 1998 | 9603887 |
| phenotypic and genotypic characterization of vagococcus fluvialis, including strains isolated from human sources. | this study presents phenotypic and genotypic data for seven isolates of vagococcus fluvialis, including four strains recovered from human clinical sources, one strain isolated from an environmental source, and two strains isolated from pigs. on the basis of phenotypic characteristics, most isolates were initially classified as "unidentified enterococci," because they resembled atypical arginine-negative enterococcal species. all seven strains as well as the type strain of v. fluvialis reacted wi ... | 1997 | 9350732 |
| characterization and identification of vagococcus fluvialis strains isolated from domestic animals. | strains of vagococcus fluvialis, a species of gram-positive catalase-negative cocci, related to the genera enterococcus and carnobacterium, were isolated from various lesions of pigs, from lesions and tonsils of cattle and cats and from tonsils of a horse. most lesion strains were isolated in mixed culture from animals with disease conditions unrelated to coccal infection. certain differences with the species description of vagococcus fluvialis were found: only a proportion of the strains was mo ... | 1994 | 7989264 |
| 16s ribosomal ribonucleic acid sequence analyses of lactococci and related taxa. description of vagococcus fluvialis gen. nov., sp. nov. | the phylogenetic status of members of the genus lactococcus and some motile strains which react with lancefield group n antiserum was examined by reverse transcriptase sequencing of 16s ribosomal ribonucleic acid. in agreement with earlier nucleic acid hybridization and immunological studies of superoxide dismutase the 16s sequence data clearly demonstrate that the lactococci represent a distinct phylogenetic group equivalent in rank to the genera enterococcus and streptococcus. the motile group ... | 1989 | 2479630 |
| the in vitro effect of probiotic vagococcus fluvialis on the innate immune parameters of sparus aurata and dicentrarchus labrax. | in this study we evaluated the effect of the probiotic vagococcus fluvialis on the cellular immune unspecific system of two different fish species of great interest in aquaculture such as gilthead sea bream (sparus aurata) and european sea bass (dicentrarchus labrax). leucocytes from head kidney of the two fish species were extracted and concentration adjusted to 10(7) cells ml(-1). phagocytic and respiratory burst activity and the peroxidase content of leucocytes were observed 30 min after incu ... | 2012 | 22864109 |
| cytokine expression in head-kidney leucocytes of european sea bass (dicentrarchus labrax l.) after incubation with the probiotic vagococcus fluvialis l-21. | the european sea bass (dicentrarchus labrax l.) is one of the most extensively farmed marine fish in the mediterranean sea. under the high-density condition, common in aquaculture, the infectious diseases can cause significant economic losses. probiotics are presented as an alternative to antibiotics for the control of aquaculture diseases. this study used real-time pcr to investigate in vitro the dynamic of expression of immune-related genes in sea bass after incubation with live and inactivate ... | 2013 | 23927874 |
| microbiological changes, shelf life and identification of initial and spoilage microbiota of sea bream fillets stored under various conditions using 16s rrna gene analysis. | sea bream fillets are one of the most important value-added products of the seafood market. fresh seafood spoils mainly owing to bacterial action. in this study an exploration of initial and spoilage microbiota of sea bream fillets stored under air and commercial modified atmosphere packaging (map) at 0 and 5 °c was conducted by 16s rrna gene sequence analysis of isolates grown on plates. sensory evaluation and enumeration of total viable counts and spoilage microorganisms were also conducted to ... | 2015 | 25312872 |
| characterization of the probiotic strain vagococcus fluvialis in the protection of european sea bass (dicentrarchus labrax) against vibriosis by vibrio anguillarum. | aquaculture is one of the main sources of income in many countries worldwide. intensive farms are often affected by different infectious diseases that can decrease their final production. to control this situation, several antibiotics are frequently used with known environmental consequences. the aim of this study was to analyze different bacterial strains isolated from of gilthead sea bream, sea bass, sole and meagre guts, for use as probiotics in aquaculture. the strains were evaluated in vitr ... | 2011 | 21962409 |
| vagococcus penaei sp. nov., isolated from spoilage microbiota of cooked shrimp (penaeus vannamei). | a polyphasic taxonomic study, using phenotypic, phylogenetic and genotypic characterization, was performed on five gram-stain-positive, catalase-negative, coccus-shaped vagococcus-like bacteria isolated from the spoilage microbiota of cooked shrimp. comparative 16s rrna gene sequence analysis indicated that the isolates belonged to the genus vagococcus. the five isolates shared 100% 16s rrna gene sequence similarity, and representative strain cd276(t) formed a branch that was distinct from the t ... | 2010 | 19897618 |
| new bacterial compositions in root-filled teeth with periradicular lesions. | the aim of this study was to isolate and detect microorganisms of root-filled teeth associated with periradicular lesions. specimens were sampled from patients undergoing root canal retreatment. the bacteria were characterized by morphologic and biochemical analysis and by 16s rrna gene sequencing. microorganisms were detected in 10 of 18 teeth. the majority of positive samples revealed a mixed culture of 2-8 species. in 2 teeth enterococcus faecalis was the only detected species. for the first ... | 2009 | 19166766 |
| characterization of the first oral vagococcus isolate from a root-filled tooth with periradicular lesions. | strain endo-eh was isolated from a root-filled tooth associated with periradicular lesions. after subculturing on columbia blood agar, phenotypic and genomic characterizations using different biochemical test systems, automated ribotyping, maldi-tof mass spectronomy, antibiotic susceptibility testing, and 16s rrna gene sequence analysis were applied for further analysis. phenotypic characterization identified this strain as vagococcus fluvialis. riboprint pattern analysis and 16s rrna sequencing ... | 2008 | 18560938 |
| diversity of lactic acid bacteria associated with fish and the fish farm environment, established by amplified rrna gene restriction analysis. | lactic acid bacteria have become a major source of concern for aquaculture in recent decades. in addition to true pathogenic species of worldwide significance, such as streptococcus iniae and lactococcus garvieae, several species have been reported to produce occasional fish mortalities in limited geographic areas, and many unidentifiable or ill-defined isolates are regularly isolated from fish or fish products. to clarify the nature and prevalence of different fish-associated bacteria belonging ... | 2007 | 17337536 |
| vagococcus carniphilus sp. nov., isolated from ground beef. | nine enterococcus-like strains were referred to the streptococcus laboratory at the centers for disease control and prevention (cdc) for further identification from the national antimicrobial resistance monitoring system laboratory at the cdc. the cultures were isolated from ground beef purchased from retail in oregon in 2000. conventional biochemical testing and analysis of whole-cell protein electrophoretic profiles distinguished these strains from known species of enterococci and vagococci. c ... | 2004 | 15388702 |
| vagococcus lutrae sp. nov., isolated from the common otter (lutra lutra). | phenotypic and phylogenetic studies were performed on an unknown gram-positive catalase-negative coccus isolated from a common otter (lutra lutra). comparative 16s rrna gene sequencing demonstrated that the unknown bacterium represents a new subline within the genus vagococcus, close to, but distinct from, vagococcus fluvialis and vagococcus salmoninarum. the unknown bacterium was readily distinguished from the two currently recognized vagococcus species by biochemical tests and electrophoretic ... | 1999 | 10425788 |
| the in vitro immunomodulatory effect of extracellular products (ecps) of vagococcus fluvialis l21 on european sea bass (dicentrarchus labrax) leucocytes. | the immune associated genes, interleukin-1β (il-1β), interleukin-6 (il-6), interleukin 10 (il-10), tumor necrosis factor-α (tnf-α), ciclo-oxigenase-2 (cox-2), and mx gene were studied by real-time pcr in head-kidney leucocytes of sea bass after incubation with the extracellular products (ecps) of the probiotic strain vagococcus fluvialis l21 and polyinosinic:polycytidylic acid (poly i:c), at different times (t1.5, t6, t12, t24, t48 and t72). in general, we can observe how pro-inflammatory cytoki ... | 2015 | 25485483 |
| isolation and characterization of vagococcus sp from midgut of culex quinquefasciatus (say) mosquito. | mosquito gut is a rich source of microorganisms. these microorganisms exhibit close association and contribute various physiological processes taking place in mosquito gut. the present study is aimed to characterize two bacterial isolates m19 and gb11 recovered from the gut of culex quinquefasciatus mosquito collected from bhuj and jamnagar districts of gujarat, india. | 2015 | 25815867 |