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adherence of bacterial to vaginal epithelial cells.vaginal epithelial cells from healthy women were washed and incubated in tissue culture medium with freshly isolated bacteria of the indigenous vaginal flora and with bacteria of species that have been discussed in conjunction with genital infections. after incubation and washing, the number of bacteria that adhered per cell was determined. the influence on the attachment rate of such factors as variations in the washing procedure, bacterial density, and incubation time was assessed. lactobacill ...19765372
microbiology of the female genital tract during pregnancy and parturition. 197610196
[unspezific gynaecological infections - changes in diagnosis and therapy over the last 100 years (author's transl)]. 197940367
ineffectiveness of erythromycin for treatment of haemophilus vaginalis-associated vaginitis: possible relationship to acidity of vaginal secretions.to assess the efficacy of oral erythromycin in the treatment of nonspecific vaginitis (nsv), conducted a nonrandom, unblinded pilot study among 17 women with symptoms and signs of nsv. at the completion of treatment, 10 of 13 patients had persistent symptoms, 9 of 13 had persistent abnormal discharge, and 11 of 13 had persistently positive cultures for haemophilus vaginalis. ten patients with persistent or relapsing nsv and four who did not complete erythromycin treatment were retreated with ora ...197943114
corynebacterium vaginale bacteraemia in a man. 197876912
haemophilus vaginalis in nonspecific vaginitis. 197879813
microbiology of the vagina in children: normal and potentially pathogenic organisms.vaginal cultures from 100 healthy girls, 2 months to 15 years of age, were examined for the presence of normal and potentially pathogenic microorganisms. corynebacterium vaginale, yeast species, and genital mycoplasmas were isolated from vaginal cultures from 13.5 %, 28%, and 28% of the girls examined, respectively. colonization with these organisms was not associated with signs or symptoms of vaginitis. neisseria gonorrhoeae was isolated from a 4-year-old with purulent vaginitis. trichomonas va ...197898750
nonenteric gram negatives, 1976. 1979109001
sensitivity of haemophilus vaginalis (corynebacterium vaginale) to oleandomycin and spectinomycin.haemophilus vaginalis is highly sensitive to oleandomycin. the mic ranged from 0.035 to 0.15 mug/ml. the sensitivity of h. vaginalis to spectinomycin was 2.5-5.0 mug/ml. the sensitivity of h. vaginalis to spectinomycin is 3-4 times higher than that of neisseria gonorrhoeae. a favourable effect in the treatment of vaginitis caused by h. vaginalis could thus be expected.1975125402
the mode of regulation of bacterial citrate synthase as a taxonomic tool. 1975168310
importance of the so-called 'other' sexually-transmitted diseases.(1) some data are presented concerning the frequency and potential morbidity of sexually-transmitted organisms other than t. pallidum or n. gonorrhoeae. (2) most of the diseases with which these organisms are associated are more prevalent than syphilis and some, at least in one sex, are as common as gonorrhoea. a number appear to carry considerable morbidity, which in the case of type ii herpes virus--if it is responsible for cervical cancer--may ultimately cause more fatalities than syphilis. ( ...1975168937
search for uro-genital tract infections in patients with symptoms of prostatitis. studies on aerobic and strictly anaerobic bacteria, mycoplasmas, fungi, trichomonads and viruses.seventy-nine patients with symptoms of nonacute prostatitis and 20 healthy volunteers were examined for uro-genital tract infection with bacteria, mycoplasmas, fungi, trichomonads and viruses. no differences in the results of the bacterial cultures were found between the patients and the controls. in only a few cases were established urinary tract pathogens found, but in no instance were these findings reproducible in later specimens. the cultures of the expressed prostatic fluids and the sample ...1975175434
managing common vulvovaginal diseases. 1976185319
immunological and chemical characterization of the extracellular antigens from corynebacterium vaginale.supernatants from 72-h peptone-starch-dextrose broth cultures of corynebacterium vaginale contained significant quantities of three extracellular, soluble antigens (esa). the esa were concentrated and partially purified by dialysis followed by ammonium sulfate and ethanol precipitation. diethylaminoethylcellulose columns were used to isolate two of the three esa. the esa were shown to be similar to antigens found on whole c. vaginale cells. absorption studies indicated that the cell antigens are ...1977200561
diagnosis and etiology of nongonococcal urethritis.the observation of more than four polymorphonuclear cells (pmn) per high-power field (hpf) in gram-stained smears of urethral secretions was found to differentiate patients with urethritis from patients without urethritis. a urethral discharge was present in 78% of patients with nongonococcal urethritis (ngu). dysuria without demonstrable urethral discharge and with fewer than four pmn/hpf did not appear to fit into the ngu spectrum. ngu is now defined to include men who have negative urethral c ...1978213495
effect of silver nitrate application on the conjunctival flora of the newborn: and the occurrence of clostridial conjunctivitis.newborn conjunctival cultures were obtained from 35 babies prior to silver nitrate application and 48 hours later. on initial culture, 46 facultative bacteria and 27 anaerobes were recovered; 48 facultative and 18 anaerobes were recovered after 48 hours. haemophilus vaginalis, bacteroides species and anaerobic cocci decreased in numbers, whereas s. epidermidis, micrococcus and propionibacterium acnes increased during this time interval. clostridial species were isolated from two cases who develo ...1978216788
vaginitis. 1979220473
trichomoniasis, candidiasis, and corynebacterium vaginale vaginitis. 1979293511
starch serum agar--a differential medium for the isolation of corynebacterium vaginale ('haemophilus' vaginalis) 1977300381
heamophilus vaginalis (corynebacterium vaginal): method for isolation and rapid biochemical identification. 1977300733
comparisons between direct microscopic and cultural methods for recognition of corynebacterium vaginale in women with vaginitis.the frequency with which clue cells could be detected in gram-stained vaginal smears and/or cervical papanicolaou (pap) smears was compared with the frequency of corynebacterium vaginale (haemophilus vaginalis) isolation in a group of 236 female patients, of whom 221 had vaginitis. vaginal clue cells were found most often in women from whom c. vaginale was isolated (p = 0.00006) whereas, conversely, clue cells in cervical pap smears were reported more frequently in women with negative cultures f ...1977300740
new differential medium for the isolation of corynebacterium vaginale.a new differential medium for isolation of corynebacterium vaginale is described. this opaque medium containing 1% corn starch allows detection of c. vaginale by the zones of clearing developing around the colonies.1977300741
etiology of nongonococcal urethritis. evidence for chlamydia trachomatis and ureaplasma urealyticum.chlamydia trachomatis, ureaplasma urealyticum (t-mycoplasma), and hemophilus vaginalis have previously been considered possible etiological agents in nongonococcal urethritis (ngu). in this study, current c. trachomatis infection was confirmed by culture and (or) micro-immunofluorescence serology in 26 of 69 men experiencing afirst episode of ngu, and 1 of 39 with no urethritis. serum igm immunofluorescent antibody to chlamydia was demonstrated in 16 of 20 men with chlamydia culture positive ngu ...1977300742
vaginal colonization with corynebacterium vaginale (haemophilus vaginalis).vaginal cultures for corynebacterium vaginale and confidential questionnaires were obtained from unselected young women who consulted a gynecologist in a student health service. in all, 466 women were studied, 150 (32.2%) of whom were colonized with c. vaginale. logit analysis defined four factors that were significantly associated with colonization with c. vaginale: nonwhite race, use of oral contraceptives, no history of marriage, and a history of pregnancy. sexual experience had little influe ...1977303656
haemophilus vaginalis (corynebacterium vaginale) vaginitis in women attending public health clinics: response to treatment with ampicillin.haemophilus vaginalis (corynebacterium vaginale) was isolated from genital specimens from 150 women who came to a public health venereal disease clinic. forty-six patients returned to the clinic for at least one follow-up examination. of the returnees, 22 of 24 who initially had received adequate therapy (14 g ampicillin in seven days) were cured, compared with three of 22 who did not received adequate therapy (x2 = 25.11; p less than 0.00005). these data included significant cure rates with ade ...1978306133
nonspecific vaginitis: role of haemophilus vaginalis and treatment with metronidazole.to assess the cause of nonspecific vaginitis, we performed a prospective case-control study of vaginal flora and a randomized unblinded trial of different therapies. haemophilus vaginalis was isolated from 17 to 18 women with signs of vaginitis but only one of 18 normal matched controls (p less than 0.002). the concentration of anaerobic bacteria in vaginal washings also was increased in patients. clinical improvement and eradication of h. vaginalis occurred in one of seven patients given sulfon ...1978306577
hemophilus vaginalis vaginitis and gonorrhea in pregnancy. 1978307063
salient features of haemophilus vaginalis.a total of 78 strains of haemophilus vaginalis were examined for 104 features. all strains fermented dextrin, maltose, and starch. additionally, more than 90% of the strains fermented galactose, glucose, and ribose. arbutin, cellobiose, melibiose, rhamnose, and salicin were not fermented by any of these strains. none of the strains acidified any of 14 alcohols or alkalinized any of 25 organic salts and amides. more than 90% of the strains hemolyzed human blood agar and hydrolyzed hippurate. no s ...1979311779
veneral diseases. 1979313576
antibiotic susceptibility of haemophilus vaginalis (corynebacterium vaginale) to 21 antibiotics.a total of 56 strains of haemophilus vaginalis were tested for their in vitro susceptibility to 21 antimicrobial agents by an agar dilution method. all strains were inhibited by 1 mug or less of penicillin, ampicillin, carbenicillin, and vancomycin per ml. the cephalosporins were less active; 4 mug of cefazolin per ml, 16 mug of cephalothin per ml, or 128 mug of cephalexin per ml was required to inhibit all strains. kanamycin, gentamicin, tobramycin, and neomycin were relatively inactive against ...1979314776
normal vaginal flora. 1979314828
comparison of two media for isolation of haemophilus vaginalis.starch agar and v agar were comparable in the isolation of haemophilus vaginalis from vaginal discharge specimens. each medium had certain advantages over the other, which are described.1979315412
role of hemophilus vaginalis in vaginitis. 1979315713
tellurite reduction test to aid in the recognition of corynebacterium vaginale.corynebacterium vaginale (haemophilus vaginalis) does not reduce potassium tellurite. when a 1% aqueous solution of tellurite is added to starch agar plates previously inoculated with vaginal discharge material, other starch-fermenting and most non-starch-fermenting bacteria rapidly reduce tellurite to produce black or gray colonies. this test is a useful adjunct to methods for rapid presumptive identification of c. vaginale. c. vaginale is more susceptible to tellurite inhibition than a variety ...1977323282
imidazo[4,5-f]quinolines iii: antibacterial 7-methyl-9-(substituted arylamino)imidazo[4,5-f]quinolines.a series of 7-methyl-9-(substituted arylamino)imidazo[4,5-f]quinolines was prepared and screened against four varieties of bacteria. the compounds possessed antibacterial activity against both gram-positive and gram-negative organisms.1977330837
corynebacterium vaginale.corynebacterium vaginale is a sexually transmitted organism which was first recognized in 1953. it appears to utilize glycogen stored in vaginal epithelial cells, causing a malodorous vaginal discharge characterized by an abnormally high ph (5.0 to 5.5) and composed mainly of epithelial cells and hordes of bacilli. infected men are asymptomatic, carry the organism for an unknown period of time, and transmit it through intercourse. the organism requires five b-vitamins, purines, pyrimidines, and ...1977333628
[problems in paediatric gynaecology (author's transl)].the present survey deals with the physiological development and pathological disturbances of the female genital organs in childhood. the different phases in development of the female genitals are discussed under sections on the newborn phase, the resting phase and the maturing time. then follows a description of the most important illnesses met with in practice including various types of vulvovaginitis, tumours, genital bleeding and accidental injuries. this review could not be extended to inclu ...1978344132
[isolation of haemophilus vaginalis in patients with leukorrhea].strains of haemophilus vaginalis were isolated from patients with leukorrhea. the characteristics of the microorganism that permit its laboratory diagnosis and results of the antibiotic-sensibility tests are described.1977354641
[comparison of 4 culture media for the identification and isolation of haemophilus vaginalis]. 1978363534
factors affecting isolation and identification of haemophilus vaginalis (corynebacterium vaginale).the rate of isolation of organisms resembling haemophilus vaginalis (corynebacterium vaginale) from vaginal specimens was not significantly affected by anaerobic versus carbon dioxide incubation atmospheres or whether specimens were inoculated on isolation media immediately after collection or after a delay of 6 h. forty-one clinically isolated strains were provisionally divided into 30 h. vaginalis strains and 11 h. vaginalis-like (hvl) strains based on morphological and growth characteristics. ...1979372217
[isolation of haemophilus equigenitalis from the cervical swab of a halfbred mare (author's transl)]. 1979383445
[experiences with contagious equine metritis (cem 77) (author's transl)]. 1979383449
inhibition of haemophilus vaginalis (corynebacterium vaginale) by metronidazole, tetracycline, and ampicillin.the minimal inhibitory concentrations (mics) of ampicillin, tetracycline, and metronidazole for 71 strains of haemophilus vaginalis (corynebacterium vaginale) were compared by use of an agar-dilution method and an inoculum of 10(6) organisms/ml. all strains were sensitive to 1 microgram of ampicillin/ml, 70% to 4 micrograms of tetracycline/ml, and only 13% of the strains to 8 micrograms of metronidazole/ml. under anaerobic conditions the susceptibility to metronidazole increased markedly, and 48 ...1979388682
aerobic and anaerobic bacterial flora of the maternal cervix and newborn gastric fluid and conjunctiva: a prospective study.aerobic and anaerobic cultures were performed on cervical swabs from 35 mothers and their newborn infants' gastric aspirates and conjunctival sacs. four hundred seventy-nine isolates were obtained; 287 were aerobes and 192 were anaerobes. the cervical and gastric cultures overall yielded a similar flora, but this was not always the case when one compared mothers with their own newborn infants. the conjunctival cultures yielded about half of the number of bacteria per specimen that the gastric co ...1979440845
amine content of vaginal fluid from untreated and treated patients with nonspecific vaginitis.we examined the vaginal washings from patients with nonspecific vaginitis (nsv) to seek biochemical markers and possible explanations for the signs and symptoms of this syndrome. seven amines were identified including methylamine, isobutylamine, putrescine, cadaverine, histamine, tyramine, and phenethylamine. these amines may contribute to the symptoms of nsv and may contribute to the elevated ph of the vaginal discharge. they may also be partly responsible for the "fishy" odor that is character ...1979447831
[vaginitis caused by corynebacterium vaginale (author's transl)]. 1979449590
[aminopeptidase activity in "corynebacterium vaginale" (author's transl)].aminopeptidase activity can only be detected in significant amounts in gram-negative bacteria with a test reaction using l-alanine-4-nitro-anilide as substrate. corynebacterium vaginale show no aminopeptidase activity even after prolonged reaction times. this indicates surely that this microorganism is nt a gram-negative bacteria.1979533075
comparative isolation of vaginal yeasts on selective and nonselective media.the isolation of vaginal yeasts was compared on a selective medium, phosphomolybdic acid agar, and on starch agar, a nonselective differential medium used primarily to isolate corynebacterium vaginale. the majority of the candida albicans strains were isolated on starch agar, but the selective medium was required for isolating all yeasts from the greatest number specimens.1976780374
polymicrobial bacteremia in obstetric patients.thirteen cases of polymicrobial bacteremia occurring in obstetric patients are reported. the most commonly occurring combination involved the bacteriodeaceae, anaerobic streptococci, and hemophilus vaginalis. in 3 cases the spectrum of bacterial isolates obtained from the intravascular compartment changed significantly.1976781573
quantitation of neisseria gonorrhoeae from women with gonorrhea.the number of infecting organisms has been shown to be an important variable in animal models of infection with neisseria gonorrhoeae. this variable may also be important in the natural transmission of gonorrhea in humans. the number of gonococci in the cervicovaginal area of women with gonorrhea is unknown, as are the effects of certain physiologic or therapeutic variables on the number of gonococci. in this study a semiquantitative technique for the enumeration of n. gonorrhoeae was used; 10 m ...1976819594
inhibition of corynebacterium vaginale by metronidazole.metronidazole [1-(2-hydroxyethyl)-2-methyl-5-nitroimidazole], previously thought to only inhibit obligately anaerobic bacteria, was found in vitro, to inhibit the growth of 15 facultatively anaerobic strains of corynebacterium vaginale (haemophilus vaginalis) using agar disk diffusion and broth dilution methods.1977867202
the etiology of nongonococcal urethritis in men attending a venereal disease clinic.nongonococcal urethritis was identified as a major reason that men attended our venereal disease clinic. the prevalence of several agents that might cause nongonococcal urethritis was determined. attempts were made to isolate gonococci; chlamydiae, ureaplasms urealyticum, trichmonads, candida sp., and corynebacterium vaginale from urethral swabs from 307 men. chlamydiae were recovered from 31% of the 67 men with nongonococcal urethritis compared to only 4% of 86 asymptomatic men without pyuria. ...1977867205
vaginitis. reducing the number of refractory cases.therapeutic failure in vaginitis can be minimized if all cases are properly diagnosed and specific therapy is given. use of wet mounts combined with liberal use of cultures, especially for corynebacterium vaginale, should result in an accurate diagnosis in over 90% of cases. treatment of choice for candidiasis is nystatin or miconazole nitrate applied topically. for trichomoniasis, metronidazole should be given orally to both sexual partners. ampicillin, cephalexin, or cephradine are recommended ...1977896608
bacteriology of the urethra in normal men and men with nongonococcal urethritis.sixty-nine caucasian males without a previous history of urethritis and who developed nongonococcal urethritis (ngu) and 39 similar men without urethritis (nu) were cultured from the urethra for chlamydia trachomatis, mycoplasma hominis, ureaplasma urealyticum, aerobes, and anaerobes. c. trachomatis infection was proven by culture of serology in 26 (38%) of the ngu group and 1 (3%) of the nu group; the c. trachomatis-negative ngu group had significantly more u. urealyticum (81%) than the c. trac ...1977925148
corynebacterium vaginale (haemophilus vaginalis) in women with leukorrhea.corynebacterium vaginale was recovered from vaginal-cervical swab specimens in 164 of 288 women with leukorrhea and in four of 30 women without signs or symptoms of lower genital tract infection. the amount and character of the leukorrhea observed in association with c. vaginale infection were variable; in typical cases the discharge had the appearance of a thin paste. in a series of 100 patients studied to determine the apparent causes of leukorrhea, noninfectious causes were inferred in seven ...1976984128
correspondence on the study "isolation and identification of corynebacterium vaginale (haemophilus vaginalis) in women with infections of the lower genital tract". 19751079680
obligately anaerobic strains of corynebacterium vaginale (haemophilus vaginalis).six obligately anaerobic strains of corynebacterium vaginale (haemophilus vaginalis) have been isolated and their characteristics studied. the reactions of the anaerobic strains, as well as of facultative strains tested under anaerobic conditions, are similar to the reactions previously reported for this species.19751080766
comparison of isolation of haemophilus vaginalis (corynebacterium vaginale) from peptone-starch-dextrose agar and columbia colistin-nalidoxic acid agar.a total of 447 cervical or vaginal specimens were inoculated in parallel onto peptone-starch-dextrose (psd) and columbia colistin (10 mg/ml)-nalidixic acid (15 mug/ml) (cna) agar and were incubated for 48 h at 35 degrees c in an atmosphere with 2 to 10% co2. one hundred (22.4%) of the cultures were positive for haemophilus vaginalis. forty-eight of the isolates were recovered from both psd and columbia cna agar, five from psd only, and 47 from columbia cna agar only (p less than 0.001). on colum ...19761085777
corynebacterium vaginale (hemophilus vaginalis) bacteremia: clinical study of 29 cases.twenty-nine patients with bacteremia due to corynebacterium vaginale, an inhabitant of the female genital tract, are described. four were newborn babies. nineteen were healthy young women delivered at full term by an operative procedure, cesarean section, or episiotomy. within 48 hours fever and bacteremia developed. while receiving antibiotics the fever returned to normal, usually within 48 hours. the remaining cases were associated with septic abortion, tubal pregnancy, an intrauterine device, ...19761085832
epidemiologic characteristics of women infected with corynebacterium vaginale (haemophilus vainalis).in a group of 184 women infected with corynebacterium vaginale (haemophilus vaginalis), 34% over age 30 were divorced or separated and 8% gave a history of induced abortion. fifty-one percent were taking an oral contraceptive drug, as compared to 36% of 140 women in a control group. various sexually transmitted diseases were diagnosed either concomitantly or at another time in 52% of women in the study group and 38% of those in the control group. the rate of cervical neoplasia (invasive carcinom ...19761087635
the role of fomites in the transmission of vaginitis.a role for fomites such as toilet seats in the transmission of vaginitis has never been proved or disproved. a compilation of clinical data from a university community showed that the organisms found in vaginal cultures of patients with vaginitis were, in order of frequency. candida albicans, escherichia coli, beta-hemolytic streptococci, hemophilus vaginalis and trichomonas vaginalis. in a concurrent bacteriologic survey of washroom fixtures, staphylococci and other micrococci were isolated mos ...19751092449
[role of haemophillus vaginalis in the pathogenesis of urogenital diseases].the opinion is stated, that haemophilus vaginalis is a potentially pathogenic organism found exclusively in persons with sexual intercourse, and therefore requires control tests and stimultaneous treatment of the male partner as well. further studies regarding its role in the pathogenesis of inflammatory diseases of the genito-urinary tract are necessary.19751217266
new medium for isolation of corynebacterium vaginale from genital specimens.corynebacterium vaginale was isolated from 48 (23.4%) cervical, vaginal, and urethral swabbings of 205 female outpatients. a new medium modified from peptone starch dextrose agar was used to isolate c. vaginale from most specimens. the new medium contained starch without dextrose with brom cresol purple added. with the exception of certain strains of streptococci, c. vaginale was the only organism isolated from genital specimens that grew and fermented starch on the medium. the new medium provid ...19751236613
microbiological diagnosis of bacterial vaginosis. 19921282150
the cervical papanicolaou smear: bacterial infection and the bethesda system. 19921282151
[the use of the direct method for the diagnosis of gardnerella vaginalis vaginitis].a study was carried out on 200 patients divided in two groups of 100, labeled 1 and 2. they underwent vaginal secretion tests by the direct method in order to assess its appropriateness for gardnerella vaginalis presumptive diagnosis. this study was related with patients' symptoms, such as leukorrhea and fetidity prevailing in group 1 and lower abdomen pain prevailing in group 2, as well as the proportions of germs isolated and the association with this symptom for defining the microorganism rol ...19921295010
antibacterial activity in vitro of cefpirome against clinical isolates causing sexually transmitted diseases.the in-vitro activity of cefpirome was compared with other antibiotics against organisms causing sexually transmitted diseases (std). the excellent activity of cefpirome against neisseria gonorrhoeae (mic90 1.0 mg/l), haemophilus ducreyi (mic90 0.5 mg/l), and gardnerella vaginalis (mic90 1.0 mg/l) suggests that this agent might be useful in the empirical treatment of a variety of venereal diseases.19921318294
in vitro activity of sparfloxacin and other antimicrobial agents against genital pathogens.the in vitro activity of sparfloxacin was determined for 60 strains of neisseria gonorrhoeae, 15 strains of chlamydia trachomatis and 40 strains each of gardnerella vaginalis, mycoplasma hominis, and ureaplasma urealyticum and compared with those of ampicillin, azithromycin, clarithromycin, erythromycin, ofloxacin, temafloxacin and tetracycline. sparfloxacin was active against all the strains studied and appeared to be the most potent quinolone tested. sparfloxacin had the lowest mics against n. ...19921337506
vaginal microbial flora as a cofactor in the pathogenesis of uterine cervical intraepithelial neoplasia.the vaginal microbial flora of 106 women with histopathologically confirmed cervical intraepithelial neoplasia and 79 women without disease, was evaluated for gardnerella vaginalis, trichomonas vaginalis, candida albicans and other yeasts. flora morphology was assessed by gram staining of secretions. cervical cultures were examined for herpes simplex virus, cytomegalovirus and neisseria gonorrhoeae. chlamydia trachomatis antigens in cervical secretions were detected by enzyme immunoassay. human ...19921351005
high levels of gardnerella vaginalis detected with an oligonucleotide probe combined with elevated ph as a diagnostic indicator of bacterial vaginosis.we have demonstrated a new approach to diagnosing bacterial vaginosis (bv) that is based on measuring the concentration of gardnerella vaginalis in vaginal fluid with dna probes. g. vaginalis is virtually always present at high concentrations in women who have bv but is also detected frequently in normal women, usually at concentrations of less than 10(7) cfu/ml of vaginal fluid. elevated vaginal ph is another sensitive indicator of bv, although it can occur in conjunction with other conditions. ...19921372621
physical characterization of the pore forming cytolysine from gardnerella vaginalis.the cytolytic toxin (ctox) produced by gardnerella vaginalis is able to form voltage-dependent cationic channels when incorporated in lipid membranes (moran et al. (1991) febs lett. 283, 317-320). osmotic protection experiments show that toxin incorporated in human erythrocytes forms pores between 18 a and 28 a in diameter. a hypothesis of pore formation as a primary event to produce cytolysis is proposed. the ctox activity increases when cells are depolarized by increasing the extracellular k+ ...19921384598
a microbiological study of vaginal discharge in women attending a malaysian gynaecological clinic.vaginal discharge is a common complaint of women attending gynaecological clinics. the purpose of this study was to compare the occurrence of commonly implicated microorganisms in vaginal discharge amongst women with or without the complaint, attending a gynaecological and family planning clinic. the association of gardnerella vaginalis with bacterial vaginosis was also studied. it was found that there were no significant differences between the cases and controls in the isolation rate of gardne ...19921387449
[epidemiological factors and vaginal flora changes in vaginal bacteriosis (bacterial vaginosis)].the aim of this study was to know the modifications of the vaginal bacterial flora that occurs in bacterial vaginosis and to know the involvement of these microorganisms and the influence of several epidemiologic factors in the etiology of this disease.19921391016
inflammation on the cervical papanicolaou smear: the predictive value for infection in asymptomatic women.the clinical significance of inflammation on the cervical papanicolaou (pap) smear of asymptomatic women is unknown. this study assessed the possible association between inflammation on pap smears with the presence of cervical/vaginal pathogens.19921397815
a collaborative study of the in-vitro sensitivity to rp 59500 of bacteria isolated in seven hospitals in france.the in-vitro activity of rp 59500 was determined against 1051 recent clinical bacterial isolates. the susceptibility to rp 59500 was determined with an agar dilution technique for all the isolates, while mics and mbcs were determined for 82 selected strains in broth. isolates of both staphylococcus aureus and coagulase-negative staphylococci appeared to be potentially susceptible to rp 59500, independent of susceptibility to methicillin or mls resistance. (s. aureus: methicillin-sensitive, mic90 ...19921399951
heterogeneity in restriction patterns of gardnerella vaginalis isolates from individuals with bacterial vaginosis.this study was undertaken to resolve the genetic make up of gardnerella vaginalis present in bacterial vaginosis (bv). dna from several g. vaginalis isolates from within and between individual bv patients were compared by bamhi, clai and ecori restriction endonuclease analysis (rea) followed by a restriction fragment length polymorphism (rflp) study, utilizing a 5.7-kb bamhi g. vaginalis atcc14018 dna probe. four g. vaginalis isolates from one patient (gvp-062) were composed of 3 different bioty ...19921410795
infection and labor. viii. microbial invasion of the amniotic cavity in patients with suspected cervical incompetence: prevalence and clinical significance.the purpose of this study was to determine the prevalence and clinical significance of microbial invasion of the amniotic cavity in patients presenting with cervical dilatation in the midtrimester of pregnancy.19921415396
[isolation and identification of gardnerella vaginalis in women with symptoms of bacterial vaginosis].70 samples of vaginal secretions were collected from women with bacterial vaginosis syndrome, that were attended in nuevo veranillo' health center. all samples were tested in order to determinate the presence of amine and to gram'methods stain to observe the morphologic characteristics of the "clue" cells (epithelial cells with adhered bacteria). samples were cultured in a selective media of blood agar in columbia base with colistine and nalidixic acid, and incubated in an environment of co2 at ...19921439008
diagnosis of bacterial vaginosis in a routine diagnostic laboratory.vaginal swabs from 299 hospital and general practitioners' patients were examined for gardnerella vaginalis by gram film and by culture, g. vaginalis was isolated in 12% of cultures. comparison between the 'clue' cell and culture methods suggested that the former is a rapid, acceptable routine screening method for the detection of g. vaginalis. the value of the traditional method of identifying g. vaginalis by sensitivity testing is questioned. all specimens were also examined by gram film and c ...19921453913
[benzidamine in the topical treatment of vaginitis caused by gardnerella vaginalis].the present clinical trial has been carried out in order to evaluate the efficacy of benzydamine in treatment of vaginitis caused by gardnerella vaginalis. fifty women affected by this specific pathology have been submitted to topical treatment with 0.5% benzydamine vaginal cream. the treatment has been carried out for 9 consecutive days using a local application of benzydamine twice a day. the clinical result was evaluated after 12 and 60 days. the following parameters were taken into considera ...19921480306
vaginitis in non pregnant women in haryana.study was carried out in 100 patients of non-specific vaginitis (nsv) to find out the incidence of vaginitis due to g. vaginalis. out of a total of 100 subjects 20 were positive for g. vaginalis as compared to only 6 in equal number of normal matched controls. one positive specimen showed concomitant presence of c. albicans and e. coli was found in another positive specimen. presence of amines and clue cells in the discharge did not correlate with the isolation rate of g. vaginalis, thus emphasi ...19921483712
gardnerella vaginalis infection and sexual contact in female children.gardnerella vaginalis (gv) infection has been reported as being acquired via sexual contact in adults and as an indicator of sexual contact in female children (dejong, 1985). the purpose of this study was to determine if gv infection was more commonly found in 191 female children who gave a history of sexual contact and/or were infected with neisseria gonorrhoeae (gc) or chlamydia trachomatis (ct) (group 1), compared with 144 female children evaluated for possible sexual abuse and found to have ...19921486513
effects of chlorhexidine gluconate douche on normal vaginal flora.the effects of a 0.5% aqueous chlorhexidine gluconate (chg) douche on the normal vaginal flora of twenty healthy nonpregnant volunteers were investigated. the douche was applied in a premeasured 180-ml quantity daily for 7 consecutive days. specimens for aerobic and anaerobic culture of the vaginal flora were obtained on 3 occasions from each volunteer, once before and twice after chg use. the culture results were analyzed qualitatively and quantitatively. a blood sample was also collected withi ...19921487182
gardnerella vaginalis: characteristics, clinical considerations, and controversies.the clinical significance, gram stain reaction, and genus affiliation of gardnerella vaginalis have been controversial since gardner and dukes described the organism as the cause of "nonspecific vaginitis," a common disease of women which is now called bacterial vaginosis. the organism was named g. vaginalis when taxonomic studies showed that it was unrelated to bacteria in various genera including haemophilus and corynebacterium. electron microscopy and chemical analyses have elucidated the org ...19921498765
mycoplasma hominis in women with bacterial vaginosis.an association between m. hominis and bacterial vaginosis (bv) was looked for in total 205 women characterized as having bv. vaginal specimens from these patients were studied for the presence of amines, polymorphonuclear leukocytes, clue cells, and lactobacilli, and were also cultured to isolate gardnerella vaginalis, m. hominis, trichomonas vaginalis, candida albicans and anaerobes. g. vaginalis was found to be the most common isolate from 132 cases (64.3%). m. hominis was isolated from 90 cas ...19921506065
prenatal microbiological risk factors associated with preterm birth. 19921525111
diagnosis of bacterial vaginosis in a gynaecology clinic.to estimate the prevalence of bacterial vaginosis in women referred to a gynaecology clinic, and to compare two methods of diagnosing bacterial vaginosis.19921547176
sialidases (neuraminidases) in bacterial vaginosis and bacterial vaginosis-associated microflora.bacterial vaginosis, prevotella species, and bacteroides species have been associated with prematurity and upper genital tract infection. prevotella (bacteroides) species and bacteroides fragilis have also been associated with preterm birth. however, the mechanism by which lower genital tract infection causes upper genital tract disease remains poorly understood. sialidases (neuraminidases) are enzymes which enhance the ability of microorganisms to invade and destroy tissue. elevated levels of s ...19921551983
[prevalence of g. vaginalis, mycoplasma, ureaplasma, t. vaginalis, yeast, n. gonorrhoeae and other bacteria in women with vaginal discharge].vaginal discharge of 118 women attended to outdoor clinics of obstetrics and gynaecology at medical faculty of ankara university (a.u.t.f.), have been examined. direct microscopy has been made by means of wet mount, gram and giemsa staining. vaginal ph has been measured, by 10% koh the presence of "fishy odor" has been investigated. for culturing vaginal secretions in amies transport medium have been brought to microbiology department of ankara university, faculty of medicine inoculations have b ...19921588849
comparison of the vaginal flora in sexually abused and nonabused girls.the presence of sexually transmitted pathogens in the vagina of the sexually abused girl may provide direct evidence of sexual abuse; the presence of other abnormal vaginal organisms may provide indirect evidence of abuse. to identify abnormal vaginal organisms, we prospectively studied the flora of 209 sexually abused girls (cases) and compared it with that in a concurrent control group of 108 girls. case and control subjects were from the same community, were predominantly white, and 71% of ea ...19921593345
report on gardnerella vaginitis.gardnerella vaginitis is one of the most common diagnoses in our outpatient clinic. from january 1, 1989 through july 30, 1989 we had 230 positive cultures for g. vaginitis. eighty-three compliant patients in this group had unabated symptoms with documented positive cultures for gardnerella after treatment with metronidazole. this led to the use of alternative regimens of ceftriaxone or clindamycin. a survey of antibiotic choice and post-treatment culture obtained 103 entries from 34 active duty ...19921603376
prenatal microbiological risk factors associated with preterm birth.to study the vaginal flora of pregnant women at 22-28 weeks gestation to determine whether the presence of specific micro-organisms is significantly associated with preterm birth and prelabour rupture of the membranes.19921606115
comparison of ofloxacin and metronidazole for the treatment of bacterial vaginosis.the efficacy and safety of ofloxacin, 200 mg twice daily for 7 days, was compared with metronidazole, 400 mg twice daily for 7 days, for the treatment of bacterial vaginosis (bv). diagnosis of bv was confirmed by at least 3 of the following 4 criteria: the presence of an abnormal vaginal discharge on examination, clue cells on microscopy of vaginal specimens, vaginal ph greater than 5.0 and a positive amine test. vaginal specimens were examined for mobiluncus spp, analysed for the succinate/lact ...19921616967
treatment for gardnerella vaginitis. 19921620523
the effects of a combined contraceptive vaginal ring releasing ethinyloestradiol and 3-ketodesogestrel on vaginal flora.fifty nine women with documented normal ovulatory cycles and with no symptoms of vaginal infection were divided into four groups. each group used a combined contraceptive vaginal ring (ccvr) with a mean daily release rate of 0.015 mg of ethinyloestradiol (ee) and 0.120 mg of 3-ketodesogestrel (3-kdg) per day, for one cycle of either 21, 28, 42, or 56 days. cultures from the posterior vaginal fornix and from the endocervical canal were obtained immediately before insertion of the ring and on remo ...19921623721
involvement of gardnerella vaginalis in urinary tract infections in men.fifteen male patients from whose urine samples gardnerella vaginalis was isolated (clinical incidence of 0.1%) were evaluated for clinical signs and symptoms of urinary tract infection and modality of acquisition of the organism. ten of 15 (67%) patients were symptomatic or had signs of inflammation as manifested by an increased number of urinary neutrophils. one patient had two bouts of infection caused by this organism which required two courses of antibiotic therapy. colonies of diphtheroidli ...19921624577
control of the microbial flora of the vagina by h2o2-generating lactobacilli.h2o2-generating lactobacilli (lb+) are present in the vagina of most normal women but are absent from most women with bacterial vaginosis (bv). lb+ at high concentration was toxic to gardnerella vaginalis (the predominant organism in the vagina of women with bv); when the lb+ was lowered to a level where it was ineffective alone, the addition of myeloperoxidase and chloride reinstituted toxicity. toxicity was inhibited by catalase and was not seen when h2o2-negative lactobacilli were used, impli ...19911647428
the in-vitro activity of temafloxacin compared with other antimicrobial agents.temafloxacin, like other 4-quinolones, was highly active against most isolates of enterobacteriaceae. it was slightly less active than ciprofloxacin but approximately as active as ofloxacin and enoxacin. it was the most active of the quinolones against acinetobacter spp. and xanthomonas maltophilia but was slightly less active than ciprofloxacin against pseudomonas spp. temafloxacin was highly active against haemophilus influenzae, moraxella catarrhalis and neisseria gonorrhoeae and was the most ...19911657855
nonoxynol-9: differential antibacterial activity and enhancement of bacterial adherence to vaginal epithelial cells.the antibacterial activity and adherence-enhancing effects of nonoxynol-9 were evaluated against vaginal and uropathogenic bacteria. nonoxynol-9 was markedly less active against the 43 uropathogenic bacterial and yeast strains tested (mic90, greater than 32%) than against the 26 gardnerella vaginalis strains (mic90, less than or equal to 0.015%) and the 53 lactobacillus strains (mic90, 8%) tested. hydrogen peroxide-producing strains of lactobacillus were more susceptible to nonoxynol-9 (mic90, 4 ...19911659602
a comparison of the in-vitro activity of clarithromycin, a new macrolide antibiotic, with erythromycin and other oral agents.on the basis of minimum inhibitory concentrations clarithromycin (6-o-methylerythromycin), a new macrolide, was found to be slightly more active than erythromycin against staphylococcus aureus, enterococci. moraxella catarrhalis, gardnerella vaginalis, bacteroides fragilis (sensu stricto) and b. ureolyticus and slightly less active against coagulase-negative staphylococci, alpha- and beta-haemolytic streptococci, haemophilus influenzae, campylobacter coli/jejuni and the b. melaninogenicus/oralis ...19911684981
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