| iron-dependent regulation of diphtheria toxin and siderophore expression by the cloned corynebacterium diphtheriae repressor gene dtxr in c. diphtheriae c7 strains. | a regulatory gene (dtxr) responsible for iron-dependent repression of the toxin (tox) and siderophore genes in corynebacterium diphtheriae was cloned and characterized. a dna fragment carrying dtxr repressed expression of a tox-lacz gene fusion in escherichia coli dh5 alpha in a high-iron environment but not under low-iron conditions. a protein with mobility corresponding to approximately 28 to 29 kda was identified as the product of the dtxr gene by sodium dodecyl sulfate-polyacrylamide gel ele ... | 1991 | 1828057 |
| localization of an origin of replication in corynebacterium diphtheriae broad host range plasmid png2 that also functions in escherichia coli. | subcloning and protoplast transformation studies identified a 2.6 kb fragment of corynebacterium diphtheriae plasmid png2 which contains an origin of replication (orir). molecular combination of the 2.6 kb orir cartridge with escherichia coli plasmid puc18cmr enabled the e. coli cloning vector to replicate within several species of corynebacterium host cells. a 2.6 kb plasmid formed from the orir cartridge alone is capable of replicating in e. coli. this suggests that a single origin could be us ... | 1990 | 2108894 |
| nucleotide sequence of the erythromycin resistance gene from the corynebacterium plasmid png2. | | 1990 | 2110657 |
| nucleotide sequence of the replication region from the mycobacterium-escherichia coli shuttle vector pep2. | the replication region derived from the corynebacterium diphtheriae-escherichia coli plasmid png2 was sequenced. this 1.85-kb segment contains a single origin of dna replication, one major open reading frame and shares no sequence homology with previously described plasmids. | 1990 | 2265753 |
| mapping and cloning of corynebacterium diphtheriae plasmid png2 and characterization of its relatedness to plasmids from skin coryneforms. | the relationship of plasmid png2, isolated from an erythromycin-resistant strain of corynebacterium diphtheriae, to plasmids isolated from skin coryneforms was examined. the extent of homology between plasmids from erythromycin-resistant and -susceptible skin coryneforms and png2 varied, but in aggregate homology was observed with all six bsteii fragments of png2. the data support the hypothesis that png2 originated in skin coryneforms. intact plasmid png2 and some of its restriction fragments w ... | 1986 | 3092733 |
| transformation of corynebacterium diphtheriae, corynebacterium ulcerans, corynebacterium glutamicum, and escherichia coli with the c. diphtheriae plasmid png2. | the transfection and transformation of members of two species of pathogenic corynebacteria, corynebacterium diphtheriae and corynebacterium ulcerans, is described. protoplasts were produced by treatment with lysozyme following growth in glycine, and a medium was defined on which a significant fraction of the osmotically sensitive cells were regenerated. transfections were carried out with dna from corynephage 782, a member of the beta family of converting phages, and transformations were perform ... | 1987 | 3110777 |
| the erythromycin resistance gene of the corynebacterium xerosis r-plasmid ptp10 also carrying chloramphenicol, kanamycin, and tetracycline resistances is capable of transposition in corynebacterium glutamicum. | the clinical isolate corynebacterium xerosis m82b carries the 50-kb r-plasmid ptp10 that confers resistance to the antibiotics chloramphenicol, kanamycin, erythromycin, and tetracycline. a detailed restriction map of ptp10 was constructed by cloning and analyzing restriction fragments of ptp10 in escherichia coli. the resistance determinants of ptp10 were located by studying the phenotype of the recombinant plasmids in e. coli and corynebacterium glutamicum. restriction patterns of fragments enc ... | 1995 | 7568464 |
| the dna sequence and minimal replicon of the corynebacterium glutamicum plasmid psr1: evidence of a common ancestry with plasmids from c. diphtheriae. | the complete nucleotide sequence of psr1, a 3 kb multicopy cryptic plasmid from corynebacterium glutamicum atcc 19223 has been determined. psr1 is unrelated to the 4.4 kb brevibacterium lactofermentum plasmid pbl1 and shows no dna sequence conservation with plasmids from staphylococcus. transposon insertion and deletion mutants located the minimal replicon to within a 2.1 kb ncoi-bcli restriction fragment. this region contains a single large open reading frame, orf2, flanked at the 5' end by a s ... | 1993 | 8409918 |
| the corynebacterium xerosis composite transposon tn5432 consists of two identical insertion sequences, designated is1249, flanking the erythromycin resistance gene ermcx. | analysis of the 50-kb r-plasmid ptp10 from the clinical isolate corynebacterium xerosis m82b revealed that the erythromycin resistance gene, ermcx, is located on a 4524-bp composite transposable element, tn5432. the ends of tn5432 are identical, direct repeats of an insertion sequence, designated is1249, encoding a putative transposase of the is256 family. is1249 consists of 1385 bp with 45/42 imperfect terminal inverted repeats. the nucleotide sequence of the 1754-bp tn5432 central region is 99 ... | 1995 | 8559800 |
| insights into the genetic organization of the corynebacterium diphtheriae erythromycin resistance plasmid png2 deduced from its complete nucleotide sequence. | the complete nucleotide sequence of the erythromycin resistance plasmid png2 from the human pathogen corynebacterium diphtheriae s601 was determined. the plasmid has a total size of 15,100 bp and contains at least 17 coding regions. comparative genomics identified conserved motifs within replication initiator proteins of corynebacterial plasmids and a novel nucleotide sequence feature, termed 22-bp box, located downstream of the repa gene. the erythromycin resistance determinant erm(x) is flanke ... | 2003 | 12584002 |