| genetic characterization of kanamycin resistance in campylobacter coli. | the restriction map of plasmid pip1433 from campylobacter coli bm2509, isolated in france, was constructed and the location of the kanamycin (km) and tetracycline (tc) resistance markers determined using probes for apha-3 and teto genes, respectively. these dna probes were used to study a second km- and tc-resistant strain c. coli ua696, isolated in canada. in this strain, the km resistance determinant was located in the chromosome. like pip1433, the km resistance determinant in c. coli ua696 sp ... | 1988 | 2855308 |
| in vivo transfer of genetic information between gram-positive and gram-negative bacteria. | a 1427-bp dna fragment containing the kanamycin resistance gene, apha-3, of plasmid pip1433 from campylobacter coli was inserted into a shuttle vector. full expression of apha-3 was obtained in bacillus subtilis and in escherichia coli. this dna fragment was sequenced in its entirety and the starting point for apha-3 transcription in b. subtilis, c. coli and e. coli was determined by s1 nuclease mapping. the sequence of the promoter consists of the hexanucleotides ttgaca and tataat, with a spaci ... | 1985 | 3937729 |