| cloning and sequencing of a plasmid-borne gene (opd) encoding a phosphotriesterase. | plasmid pcms1 was isolated from pseudomonas diminuta mg, a strain which constitutively hydrolyzes a broad spectrum of organophosphorus compounds. the native plasmid was restricted with psti, and individual dna fragments were subcloned into pbr322. a recombinant plasmid transformed into escherichia coli possessed weak hydrolytic activity, and southern blotting with the native plasmid dna verified that the dna sequence originated from pcms1. when the cloned 1.3-kilobase fragment was placed behind ... | 1988 | 2834339 |
| indigenous organophosphate-degrading (opd) plasmid pcms1 of brevundimonasdiminuta is self-transmissible and plays a key role in horizontal mobility of the opd gene. | a fosmid library of the 66kb indigenous organophosphate-degrading (opd) plasmid pcms1 of brevundimonas diminuta was tagged with mini-transposon eztn5 <r6k?ori/kan-2>, to determine its sequence using transposon-specific primers. the sequence revealed the presence of a number of tra genes suggesting their role in conjugal transfer of pcms1. consistent with the presence of the tra genes, the b. diminuta plasmid, pcms1::tet, generated by replacing the opd gene with opd::tet, served as a donor for tr ... | 2011 | 21354204 |