| nucleotide sequence of the chloramphenicol acetyltransferase gene of streptomyces acrimycini. | the nucleotide sequence of a gene (cat) encoding chloramphenicol acetyltransferase (cat) in streptomyces acrimycini was determined. the predicted amino acid sequence demonstrates extensive homology with those of cats isolated from gram-negative enterobacteria, notably with the type iii variant encoded by the inck plasmid r387. transcript mapping indicates a single cat mrna with a 5' end coinciding with the aug codon used for translational initiation in vivo. we also determined the extent of a sp ... | 1989 | 2697637 |
| genetic and molecular characterization of r plasmids incompatible with r387 (inck). | more than 70 conjugative r plasmids have been isolated from wild-type strains originating mainly in south-east europe and identified as incompatible with the reference plasmid r387 of the incompatibility group k. these plasmids, governing different resistance patterns, have been characterized as p1 cotransducible species of between 50 and 60 mega-daltons. in contrast to the genetic similarity between all these plasmids and the reference type r387, their dna revealed different digestion patterns ... | 1980 | 7003060 |
| nucleotide sequence analysis and overexpression of the gene encoding a type iii chloramphenicol acetyltransferase. | the gene catiii, encoding a type iii enterobacterial chloramphenicol acetyltransferase, was cloned from the transmissible plasmid r387 into pbr322 and bacteriophage m13 mp8. nucleotide sequence analysis of 1160 bp of dna identified an open reading frame encoding a protein of 213 amino acid residues and a calculated molecular mass of 24965 da. the predicted n-terminal sequence is identical with that determined by edman degradation of chloramphenicol acetyltransferase purified from escherichia col ... | 1988 | 3048245 |
| mobilization of r387 inc k conjugative plasmid by the conjugative plasmid r64 inc i. | plasmid aggregate (r387, r64) was constructed in e. coli k12 strain. plasmid r387 inc k was stimulated to conjugational transfer by plasmid r64 inc i. this stimulation was caused neither by recombination between both plasmids nor by trans-complementation of r387 conjugational systems by gene(s) product(s) of r64 plasmid. the observed phenomenon resembled rather mobilization of nonconjugative plasmids by conjugative ones. as in mobilization, the observed increase in r387 transfer frequency could ... | 1989 | 2482657 |