| transposable plasmid deoxyribonucleic acid sequence in pseudomonas aeruginosa which mediates resistance to gentamicin and four other antimicrobial agents. | a 9.1 x 10(6)-dalton transposable deoxyribonucleic acid sequence resides within pseudomonas aeruginosa plasmid r1033 and mediates resistance to gentamicin, streptomycin, sulfamethoxazole, chloramphenicol, and mercuric chloride. transposability was demonstrated in escherichia coli when this sequence, designated tn1696, excised from r1033 and integrated into plasmid pmb8. excision and insertion of tn1696 occurred independently of the host rec phenotype and may involve the 140-base pair, inverted d ... | 1979 | 113388 |
| construction of a tn5 derivative determining resistance to gentamicin and spectinomycin using a fragment cloned from r1033. | a physical and genetic map of the incp plasmid r1033 was constructed: restriction fragments were subcloned and antibiotic resistance genes were located. the map is consistent with previous reports that r1033 is a derivative of rp4 carrying a 16-kb transposon tn1696 which contains the antibiotic-resistance determinants present on r1033 but not on rp4. a bamhi fragment from r1033, determining resistance to gentamicin, spectinomycin and streptomycin, was cloned into tn5, replacing the central bg/ii ... | 1986 | 3030896 |