| a stable core region of the tra operon mrna of plasmid r1-19. | the degradation of the polycistronic tra-mrna of the resistance plasmid r1-19 leads to the accumulation of a well defined series of stable mrna species. the majority of the most stable mrnas contains the message for the traa gene only. the differently sized stable mrnas possess a common 3'terminus within the tral gene but vary at their 5' ends. the 3'terminus probably results from protection against exoribonucleases by a secondary structural feature. we propose that the 5' ends are generated by ... | 1989 | 2564189 |
| plasmid control of recombination of e. coli k12. | the recombination proficiency of three recipient strains of escherichia coli k12 carrying different plasmids was investigated by conjugal mating with hfr cavalli. some plasmids (e.g. r1drd 19, r6k) caused a marked reduction in the yield of recombinants formed in crosses with hfr but did not reduce the ability of host strains to accept plasmid f104. the effect of plasmids on recombination was host-dependent. in hfr crosses with ab1157 (r1-19) used as a recipient the linkage between selected and u ... | 1980 | 6258017 |
| differential mrna decay within the transfer operon of plasmid r1: identification and analysis of an intracistronic mrna stabilizer. | processing of the transfer operon mrna of the conjugative resistance plasmid r1-19 results in the accumulation of stable traa mrnas. the stable traa transcripts found in vivo have identical 3' ends within downstream tral sequences, but vary at their 5' ends. the 3' ends determined coincide with the 3' base of a predicted large clover-leaf-like rna secondary structure. here we demonstrate that this putative rna structure, although part of a coding sequences, stabilizes the upstream traa mrna very ... | 1996 | 8602164 |
| [identification of the plasmid r1drd-19 region complementing the mutant phenotype recb- in escherichia coli k12 strain]. | plasmid r1-19 and its copy number mutants markedly increase the recombinational efficiency of a recb- strain of e. coli k12 and its resistance to the lethal action of uv and mitomycin c. these effects are associated with the appearance of a new atp-dependent exonuclease activity in recb- cells known to be deficient in the atp-dependent exonuclease v. using hybrid plasmids carrying different ecori fragments of r1-19 (in the psf124 vector), the gene(s) responsible for effect of r1-19 in recb-cells ... | 1985 | 3025684 |