| nitric oxide reductases of prokaryotes with emphasis on the respiratory, heme-copper oxidase type. | the investigation of respiratory n-oxide reduction as part of a biogeochemical process sustained by prokaryotes has roots over a century ago and has laid the groundwork for microbial nitric oxide (no) biology and recognizing that no is of bioenergetic importance as an electron acceptor in anaerobic environments. no is an obligatory respiratory substrate of nitrate- and nitrite-denitrifying prokaryotes that release nitrous oxide or dinitrogen as products. we witness currently a broadening of the ... | 2005 | 15598502 |
| structural characterization of a binuclear center of a cu-containing no reductase homologue from roseobacter denitrificans: epr and resonance raman studies. | aerobic phototrophic bacterium roseobacter denitrificans has a nitric oxide reductase (nor) homologue with cytochrome c oxidase (cco) activity. it is composed of two subunits that are homologous with norc and norb, and contains heme c, heme b, and copper in a 1:2:1 stoichiometry. this enzyme has virtually no nor activity. electron paramagnetic resonance (epr) spectra of the air-oxidized enzyme showed signals of two low-spin hemes at 15 k. the high-spin heme species having relatively low signal i ... | 2004 | 15136157 |
| sequence analysis reveals new membrane anchor of reaction centre-bound cytochromes possibly related to pufx. | most of the bacterial photosynthetic reaction centres known to date contain a cytochrome subunit with four covalently bound haem groups. in the case of blastochloris viridis, this reaction centre subunit is anchored in the membrane by a lipid molecule covalently attached to the cysteine which forms the n-terminus of the mature protein after processing by a signal peptidase. we show that posttranslational n-terminal cleavage of the cytochrome subunit does not occur in the aerobic photosynthetic b ... | 2003 | 12560097 |
| photosynthetic regulatory gene cluster in an aerobic photosynthetic bacterium, roseobacter denitrificans. | | 1999 | 12501384 |
| nitric oxide-reductase homologue that contains a copper atom and has cytochrome c-oxidase activity from an aerobic phototrophic bacterium roseobacter denitrificans. | a cytochrome cb-type enzyme with cytochrome c-oxidase activity was purified from an aerobic phototrophic bacterium roseobacter denitrificans. the enzyme was solubilized with sucrose monodecanoate from the membranes of r. denitrificans grown aerobically under light conditions, and purified to electrophoretic homogeneity. absorption spectra of the purified enzyme showed peaks at 410 nm and 530 nm in the oxidized state, and peaks at 420, 522, and 551 nm and a shoulder at around 560 nm in the reduce ... | 2002 | 12038974 |
| respiratory electron transport and light-induced energy transduction in membranes from the aerobic photosynthetic bacterium roseobacter denitrificans. | membrane fragments isolated from the aerobic phototrophic bacterium roseobacter denitrificans were examined. ninety-five percent of the total nadh-dependent oxidative activity was inhibited either by antimycin a or myxothiazol, two specific inhibitors of the cytochrome bc1 complex, which indicates that the respiratory electron transport chain is linear. in agreement with this finding, light-induced oxygen uptake, an electron transport activity catalyzed by the "alternative quinol oxidase pathway ... | 2001 | 11357509 |
| evidence for a functional similarity between the two-component regulatory systems regsr, actsr, and regba (prrba) in alpha-proteobacteria. | the symbiotic bacteria bradyrhizobium japonicum and sinorhizobium meliloti, and the purple photosynthetic bacteria rhodobacter capsulatus, rhodovulum sulfidophilum, roseobacter denitrificans and rhodobacter sphaeroides possess homologous two-component regulatory systems, namely regsr, actsr, regba and prrba. the respective response regulators of these bacteria control expression of different regulons that are involved in n2 fixation, co2 fixation, photosynthesis or acid tolerance. we therefore a ... | 2000 | 11131020 |
| differentiation of newly described antarctic bacterial isolates related to roseobacter species based on 16s-23s rdna internal transcribed spacer sequences. | the 16s-23s rdna internal transcribed spacer (its) of roseobacter denitrificans, roseobacter litoralis, ruegeria algicola and strains of the recently described species antarctobacter heliothermus and roseovarius tolerans were analysed in order to examine dna sequence variations and to draw conclusions about inter- and intraspecific relationships. a. heliothermus included four strains with an its fragment length of 1092 bp. roseovarius tolerans was described on the basis of eight strains. five of ... | 2000 | 10758903 |
| high-resolution architecture of the outer membrane of the gram-negative bacteria roseobacter denitrificans. | the outer membrane of gram-negative bacteria protects the cell against bactericidal substances. passage of nutrients and waste is assured by outer membrane porins, beta-barrel transmembrane channels. while atomic structures of several porins have been solved, so far little is known on the supramolecular structure of the outer membrane. here we present the first high-resolution view of a bacterial outer membrane gently purified maintaining remnants of peptidoglycan on the perisplasmic surface. at ... | 2009 | 19843216 |
| photo-induced cyclic electron transfer involving cytochrome bc1 complex and reaction center in the obligate aerobic phototroph roseobacter denitrificans. | flash-induced redox changes of b-type and c-type cytochromes have been studied in chromatophores from the aerobic photosynthetic bacterium roseobacter denitrificans under redox-controlled conditions. the flash-oxidized primary donor p+ of the reaction center (rc) is rapidly re-reduced by heme h1 (em,7 = 290 mv), heme h2 (em,7 = 240 mv) or low-potential hemes l1/l2 (em,7 = 90 mv) of the rc-bound tetraheme, depending on their redox state before photoexcitation. by titrating the extent of flash-ind ... | 2000 | 10632712 |
| structural and functional analyses of photosynthetic regulatory genes rega and regb from rhodovulum sulfidophilum, roseobacter denitrificans, and rhodobacter capsulatus. | genes coding for putative rega, regb, and senc homologues were identified and characterized in the purple nonsulfur photosynthetic bacteria rhodovulum sulfidophilum and roseobacter denitrificans, species that demonstrate weak or no oxygen repression of photosystem synthesis. this additional sequence information was then used to perform a comparative analysis with previously sequenced rega, regb, and senc homologues obtained from rhodobacter capsulatus and rhodobacter sphaeroides. these are photo ... | 1999 | 10400577 |
| oxygen dependence of photosynthetic electron transport in a bacteriochlorophyll-containing rhizobium. | bacteriochlorophyll-containing rhizobia, which form nitrogen-fixing nodules on the stems and roots of the legume aeschynomene, grow photosynthetically only in the presence of oxygen or auxiliary electron acceptors. we show that, in whole cells of the rhizobium strain btai 1, a single-turnover excitation flash photooxidized c-type cytochrome under aerobic but not anaerobic conditions. light-induced fluorescence yield changes show that under anaerobic conditions, the primary acceptor quinone, q(a) ... | 1997 | 9409732 |
| characterization of porin from roseobacter denitrificans. | porin from roseobacter denitrificans was isolated and purified to homogeneity. the pore characteristics from this marine bacterium were compared to those of its phylogenetically closely related freshwater bacteria rhodobacter capsulatus, rhodobacter sphaeroides and rhodopseudomonas blastica. the porin formed weakly cation-selective, general diffusion pores in lipid bilayer membranes. high transmembrane potentials caused channel closing in steps that were of one or two thirds of the initial on-st ... | 1997 | 9298192 |
| structure of the puf operon of the obligately aerobic, bacteriochlorophyll alpha-containing bacterium roseobacter denitrificans och114 and its expression in a rhodobacter capsulatus puf puc deletion mutant. | roseobacter denitrificans (erythrobacter species strain och114) synthesizes bacteriochlorophyll a (bchl) and the photosynthetic apparatus only in the presence of oxygen and is unable to carry out primary photosynthetic reactions and to grow photosynthetically under anoxic conditions. the puf operon of r. denitrificans has the same five genes in the same order as in many photosynthetic bacteria, i.e., pufbalmc. pufc, the tetraheme subunit of the reaction center (rc), consists of 352 amino acids ( ... | 1997 | 9286973 |
| complete genome sequence of a marine roseophage provides evidence into the evolution of gene transfer agents in alphaproteobacteria. | roseophage rdjlf1 is a siphovirus isolated from south china sea on roseobacter denitrificans och114. its virion encapsulates 62.7 kb genome that encodes 87 gene products. rdjlf1 shares similar genome organization and gene content with the marine bacteriophage fjl001 and pseudomonas phages yua and m6, which are different from those of typical ?- or mu-like phages. four hallmark genes (orfs 81 to 84) of rdjlf1 were highly homologous to rcgta-like genes 12 to 15. the largest gene (orf 84) was predi ... | 2011 | 21414219 |
| origin of the two carbonyl oxygens of bacteriochlorophyll a. demonstration of two different pathways for the formation of ring e in rhodobacter sphaeroides and roseobacter denitrificans, and a common hydratase mechanism for 3-acetyl group formation. | a respiring culture of rhodobacter sphaeroides, grown in the dark under defined aerobic conditions, produced cells capable of immediately commencing adaptation to photosynthetic growth on exposure to light and further reduction of oxygen tension. adaptation was complete after 12 h and the bacteriochlorophyll a content increased 10-20-fold. this adaptation was performed in the presence of either h2(18)o or 18o2. the extracted bacteriochlorophyll a was examined by mass spectrometry to determine th ... | 1996 | 8706723 |
| expression of the puf operon in an aerobic photosynthetic bacterium, roseobacter denitrificans. | the effects of oxygen and light on the expression of the puf operon were investigated in roseobacter denitrificans in a comparison with those in rhodobacter sphaeroides. in darkness, the levels of the total puf mrna in ros. denitrificans were about 1.3 times those in rb. sphaeroides at low concentrations of oxygen, reflecting the accumulation of bacteriochlorophyll and carotenoids. the oxygen tension, up to 94% saturation of dissolved oxygen, did not affect the levels of the total puf transcript ... | 1996 | 8665093 |
| some properties and occurrence of cytochrome c-552 in the aerobic photosynthetic bacterium roseobacter denitrificans. | characteristics and occurrence of cytochrome c-552 from an aerobic photosynthetic bacterium, roseobacter denitrificans, were described. relative molecular mass of the cytrochrome was 13.5 kda on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and 15,000 by gel filtration. this cytochrome was a acidic protein having a pi of 5.6 and em was +215 mv at ph 7.0. absorption peaks were at 278, 408 and 524 nm in the oxidized form and 416, 523 and 552 nm in the reduced form. amino aci ... | 1993 | 8381264 |
| structure and function of the tetraheme cytochrome associated to the reaction center of roseobacter denitrificans. | we have characterized the tetrahemic rc bound cytochrome isolated from the quasi-photosynthetic bacterium roseobacter denitrificans in terms of absorption spectrum, redox property and orientation with respect to the membrane plane. the heme, designated h1, which possesses the highest redox midpoint potential (+290 mv), absorbs at 555 nm. its plane makes an angle of 40 degrees with the membrane plane. the second high potential heme, h2 (+240 mv), peaks at 554 nm and makes a tilt of 55 degrees wit ... | 1994 | 7893818 |
| phylogenetic diversity of subsurface marine microbial communities from the atlantic and pacific oceans. | the extent of the diversity of marine prokaryotes is not well known, primarily because of poor cultivability. however, new techniques permit the characterization of such organisms without culturing, via 16s rrna sequences obtained directly from biomass. we performed such an analysis by polymerase chain reaction amplification with universal primers on five oligotrophic open-ocean samples: from 100-m (three samples) and 500-m depths in the western california current (pacific ocean) and from a 10-m ... | 1993 | 7685997 |
| peroxidase activity and involvement in the oxidative stress response of roseobacter denitrificans truncated hemoglobin. | roseobacter denitrificans is a member of the widespread marine roseobacter genus. we report the first characterization of a truncated hemoglobin from r. denitrificans (rd. trhb) that was purified in the heme-bound form from heterologous expression of the protein in escherichia coli. rd. trhb exhibits predominantly alpha-helical secondary structure and absorbs light at 412, 538 and 572 nm. the phylogenetic classification suggests that rd. trhb falls into group ii trhbs, whereas sequence alignment ... | 2015 | 25658318 |
| lipopolysaccharide and porin of roseobacter denitrificans, confirming its phylogenetic relationship to the alpha-3 subgroup of proteobacteria. | roseobacter denitrificans has rough (r)-type lipopolysaccharide, containing 2-keto-3-deoxyoctonate but no hepatoses. its lipid a has a glucosamine-containing, phosphorylated backbone. it contains the rare 3-oxotetradecanoic (3-oxomyristic) acid as the only amide-bound fatty acid and ester-bound 3-hydroxydecanoic acid, this pattern being characteristic for the alpha-3 subgroup of proteobacteria. treatment of the major outer-membrane protein (porin, apparent molecular mass 88 kda) of roseobacter d ... | 1995 | 7551064 |
| roseobacter algicola sp. nov., a new marine bacterium isolated from the phycosphere of the toxin-producing dinoflagellate prorocentrum lima. | we describe a new species on the basis of phenotypic characteristics and the results of an analysis of small-subunit rrna sequences. three strains of this organism were isolated from a culture of the toxin-producing dinoflagellate prorocentrum lima. these bacteria are gram-negative, strictly aerobic, ovoid organisms that are motile by means of one or two subpolar flagella. they grow at temperatures ranging from 10 to 37 degrees c and in the presence of nacl concentrations ranging from 0.1 to 2 m ... | 1995 | 7537061 |
| control of bacteriochlorophyll accumulation by light in an aerobic photosynthetic bacterium, erythrobacter sp. och 114. | the effect of light on bacteriochlorophyll (bchl) accumulation as well as the activity of two enzymes in the initial step of the tetrapyrrole biosynthetic pathway was examined in an aerobic photosynthetic bacterium, erythrobacter sp. strain och 114. light clearly regulated the bchl and carotenoid accumulation, completely suppressing their levels at high light intensity. however, porphyrin and bchl precursors were not found in either the cells or the growth medium of lighted culture. the level of ... | 1988 | 3056272 |
| complete amino acid sequence of cytochrome c551 from erythrobacter species strain och 114. | the complete amino acid sequence of cytochrome c551 isolated from an aerobic photosynthetic bacterium, erythrobacter sp. strain och 114, was determined. the cytochrome molecule was composed of a total of 119 amino acid residues and its molecular weight including heme was calculated to be 13,235. the sequence was (sequence: see text). its molecular weight indicates that this cytochrome is of the l-type. sequence alignment with other bacterial cytochromes c shows that this cytochrome is similar to ... | 1987 | 3038859 |
| inhibition of porphyrin biosynthesis by exogenous 5-aminolevulinic acid in an aerobic photosynthetic bacterium, erythrobacter sp. och 114. | exogenously administered 5-aminolevulinic acid (ala) inhibited the formation of bacteriochlorophyll a (bchl a) in a dose-dependent manner in the aerobic photosynthetic bacterium, erythrobacter sp. strain och 114, under dark growth conditions. the ala concentration required for half-inhibition after 24-h growth was estimated to be about 3.0 mm. porphyrin and bchl precursors were not found in either the cells or the growth medium. the same inhibition was also observed with cytochrome c formation. ... | 1988 | 2847654 |
| two types of cytochrome cd1 in the aerobic photosynthetic bacterium, erythrobacter sp. och 114. | components i and ii of cytochrome cd1 which had different spectral features were purified from the aerobic photosynthetic bacterium, erythrobacter sp. strain och 114. component i showed an absorption maxima at 700 and 406 nm in the oxidized form, and at 621, 552.5, 548 and 416 nm in the reduced form. component ii showed an absorption maxima at 635 and 410 nm in the oxidized form and at 628, 552.5, 548 and 417 nm in the reduced form. the relative molecular mass, mr, of both cytochromes was determ ... | 1989 | 2553395 |
| purification and properties of trimethylamine n-oxide reductase from aerobic photosynthetic bacterium roseobacter denitrificans. | trimethylamine n-oxide (tmao) reductase was purified from an aerobic photosynthetic bacterium roseobacter denitrificans. the enzyme was purified from cell-free extract by ammonium sulfate fractionation, deae ion exchange chromatography, hydrophobic chromatography, and gel filtration. the purified enzyme was composed of two identical subunits with molecular weight of 90,000, as identified by sds-polyacrylamide gel electrophoresis, containing heme c and a molybdenum cofactor. the molecular weight ... | 1992 | 1337081 |
| dinoroseobacter shibae gen. nov., sp. nov., a new aerobic phototrophic bacterium isolated from dinoflagellates. | a novel group of aerobic anoxygenic phototrophic bacteria was isolated from marine dinoflagellates, and two strains were characterized in detail. cells were gram-negative cocci or ovoid rods and were motile by means of a single, polarly inserted flagellum. they were obligate aerobes requiring 1-7 % salinity. the optimal ph range for growth was 6.5-9.0 and the temperature optimum was 33 degrees c. the bacteria contained bacteriochlorophyll a and spheroidenone as the only carotenoid. the in vivo a ... | 2005 | 15879238 |
| reclassification of roseobacter gallaeciensis ruiz-ponte et al. 1998 as phaeobacter gallaeciensis gen. nov., comb. nov., description of phaeobacter inhibens sp. nov., reclassification of ruegeria algicola (lafay et al. 1995) uchino et al. 1999 as marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera roseobacter, ruegeria and leisingera. | a heterotrophic, antibiotic-producing bacterium, strain t5(t), was isolated from the german wadden sea, located in the southern region of the north sea. sequence analysis of the 16s rrna gene of this strain demonstrated close affiliation with roseobacter gallaeciensis bs107(t) (99 % similarity), but the results of genotypic (dna-dna hybridization and dna g + c content) and phenotypic characterization revealed that strain t5(t) represents a novel species. the novel organism is strictly aerobic, g ... | 2006 | 16738106 |
| the complete genome sequence of roseobacter denitrificans reveals a mixotrophic rather than photosynthetic metabolism. | purple aerobic anoxygenic phototrophs (aaps) are the only organisms known to capture light energy to enhance growth only in the presence of oxygen but do not produce oxygen. the highly adaptive aaps compose more than 10% of the microbial community in some euphotic upper ocean waters and are potentially major contributors to the fixation of the greenhouse gas co2. we present the complete genomic sequence and feature analysis of the aap roseobacter denitrificans, which reveal clues to its physiolo ... | 2007 | 17098896 |
| the structure of rddddp from roseobacter denitrificans reveals that dmsp lyases in the dddp-family are metalloenzymes. | marine microbes degrade dimethylsulfoniopropionate (dmsp), which is produced in large quantities by marine algae and plants, with dmsp lyases into acrylate and the gas dimethyl sulfide (dms). approximately 10% of the dms vents from the sea into the atmosphere and this emission returns sulfur, which arrives in the sea through rivers and runoff, back to terrestrial systems via clouds and rain. despite their key role in this sulfur cycle dmsp lyases are poorly understood at the molecular level. her ... | 2014 | 25054772 |
| roseophage rdjl phi1, infecting the aerobic anoxygenic phototrophic bacterium roseobacter denitrificans och114. | a marine roseophage rdjl phi1 lytically infecting roseobacter denitrificans och114 was isolated and characterized. rdjl phi1 can package several host cellular proteins into its virions, and its dna is refractory to several commonly used restriction enzymes. this paper presents the first report of a bacteriophage isolated from the aerobic anoxygenic phototrophic bacteria. | 2009 | 19139231 |
| chimeric nitrogenase-like enzymes of (bacterio)chlorophyll biosynthesis. | nitrogenase-like light-independent protochlorophyllide oxidoreductase (dpor) is involved in chlorophyll biosynthesis. bacteriochlorophyll formation additionally requires the structurally related chlorophyllide oxidoreductase (cor). during catalysis, homodimeric subunit bchl(2) or chll(2) of dpor transfers electrons to the corresponding heterotetrameric catalytic subunit, (bchnb)(2) or (chlnb)(2). analogously, subunit bchx(2) of the cor enzymes delivers electrons to subunit (bchyz)(2). various ch ... | 2009 | 19336405 |
| carbohydrate metabolism and carbon fixation in roseobacter denitrificans och114. | the roseobacter clade of aerobic marine proteobacteria, which compose 10-25% of the total marine bacterial community, has been reported to fix co(2), although it has not been determined what pathway is involved. in this study, we report the first metabolic studies on carbohydrate utilization, co(2) assimilation, and amino acid biosynthesis in the phototrophic roseobacter clade bacterium roseobacter denitrificans och114. we develop a new minimal medium containing defined carbon source(s), in whic ... | 2009 | 19794911 |
| characterization of the photosynthetic apparatus and proteome of roseobacter denitrificans. | the phototrophic capacity of aerobic anoxygenic phototrophic bacteria endows them with a selective advantage over other heterotrophic bacteria in the oligotrophic ocean. here, we reported the phototrophic features and proteome of an aerobic phototrophic bacterium roseobacter denitrificans under starvation stress. the fluorescence induction and relaxation measurements suggested that the photosynthetic capacity in r. denitrificans was preserved but was lower than in the photoautotrophic bacterium ... | 2010 | 19826863 |
| comparative study of the gating motif and c-type inactivation in prokaryotic voltage-gated sodium channels. | prokaryotic voltage-gated sodium channels (na(v)s) are homotetramers and are thought to inactivate through a single mechanism, named c-type inactivation. here we report the voltage dependence and inactivation rate of the nachbac channel from bacillus halodurans, the first identified prokaryotic na(v), as well as of three new homologues cloned from bacillus licheniformis (na(v)bacl), shewanella putrefaciens (na(v)shep), and roseobacter denitrificans (na(v)rosd). we found that, although activated ... | 2010 | 19959480 |
| genetic tools for the investigation of roseobacter clade bacteria. | the roseobacter clade represents one of the most abundant, metabolically versatile and ecologically important bacterial groups found in marine habitats. a detailed molecular investigation of the regulatory and metabolic networks of these organisms is currently limited for many strains by missing suitable genetic tools. | 2009 | 20021642 |
| phage resistance of a marine bacterium, roseobacter denitrificans och114, as revealed by comparative proteomics. | roseobacter is a dominant lineage in the marine environment. this group of bacteria is diverse in terms of both their phylogenetic composition and their physiological potential. roseobacter denitrificans och114 is one of the most studied bacteria of the roseobacter lineage. recently, a lytic phage (rdjlphi1) that infects this bacterium was isolated and a mutant strain (m1) of och114 that is resistant to rdjlphi1 was also obtained. here, we investigate the mechanisms supporting phage resistance o ... | 2010 | 20107991 |
| anoxygenic photosynthesis and photooxidative stress: a particular challenge for roseobacter. | roseobacter clade aerobic anoxygenic phototrophic bacteria (aanp) are abundant in photic zone environments of marine ecosystems. these bacteria form a photosynthetic apparatus at oxygen saturation, a situation expected to generate high levels of singlet oxygen (¹o₂) when light is present. rhodobacter sphaeroides, an anaerobic anoxygenic phototroph, represses photosynthesis genes at high oxygen tension. here we report that roseobacter denitrificans showed higher sensitivity to ¹o₂ compared with r ... | 2010 | 21108722 |
| oxygen control of nitrogen oxide respiration, focusing on a-proteobacteria. | denitrification is generally considered to occur under micro-oxic or anoxic conditions. with this in mind, the physiological function and regulation of several steps in the denitrification of model a-proteobacteria are compared in the present review. expression of the periplasmic nitrate reductase is quite variable, with this enzyme being maximally expressed under oxic conditions in some bacteria, but under micro-oxic conditions in others. expression of nitrite and no reductases in most denitrif ... | 2011 | 21265769 |
| energy transfer in an lh4-like light harvesting complex from the aerobic purple photosynthetic bacterium roseobacter denitrificans. | a peripheral light-harvesting complex from the aerobic purple bacterium roseobacter (r.) denitrificans was purified and its photophysical properties characterized. the complex contains two types of pigments, bacteriochlorophyll (bchl) a and the carotenoid (car) spheroidenone and possesses unique spectroscopic properties. it appears to lack the b850 bacteriochlorophyll a q(y) band that is typical for similar light-harvesting complex 2 antennas. circular dichroism and low temperature steady-state ... | 2011 | 21419098 |
| molecular origins and consequences of high-800 lh2 in roseobacter denitrificans. | roseobacter denitrificans is a marine bacterium capable of using a wide variety of different metabolic schemes and in particular is an anoxygenic aerobic photosynthetic bacterium. in the work reported here we use a deletion mutant that we have constructed to investigate the structural origin of the unusual high-800 light-harvesting complex absorption in this bacterium. we suggest that the structure is essentially unaltered when compared to the usual nonameric complexes but that a change in the e ... | 2011 | 21739946 |
| comparative genome analysis and genome-guided physiological analysis of roseobacter litoralis. | roseobacter litoralis och149, the type species of the genus, and roseobacter denitrificans och114 were the first described organisms of the roseobacter clade, an ecologically important group of marine bacteria. both species were isolated from seaweed and are able to perform aerobic anoxygenic photosynthesis. | 2011 | 21693016 |
| Formation of polyhydroxyalkanoate in aerobic anoxygenic phototrophic bacteria and its relationship to carbon source and light availability. | Aerobic anoxygenic phototrophic bacteria (AAPB) are unique players in carbon cycling in the ocean. Cellular carbon storage is an important mechanism regulating the nutrition status of AAPB but is not yet well understood. In this paper, six AAPB species (Dinoroseobacter sp. JL1447, Roseobacter denitrificans OCh 114, Roseobacter litoralis OCh 149, Dinoroseobacter shibae DFL 12(T), Labrenzia alexandrii DFL 11(T), and Erythrobacter longus DSMZ 6997) were examined, and all of them demonstrated the ab ... | 2011 | 21908634 |
| Host responses of a marine bacterium, Roseobacter denitrificans OCh114, to phage infection. | RDJLF1 is a marine siphophage infecting Roseobacter denitrificans OCh114. In this study, host responses of R. denitrificans OCh114 to phage infection were investigated through in situ real-time atomic force microscopy (AFM) and proteomics approaches. As seen from the AFM observations, during phage infection processes, depression areas appeared on the host cell surface in a few minutes after infection and expanded in both diameter and depth over time and finally led to the collapse of host cells ... | 2011 | 22033766 |
| iron-sulfur cluster-dependent catalysis of chlorophyllide a oxidoreductase from roseobacter denitrificans. | bacteriochlorophyll a biosynthesis requires the stereo- and regiospecific two electron reduction of the c7-c8 double bond of chlorophyllide a by the nitrogenase-like multisubunit metalloenzyme, chlorophyllide a oxidoreductase (cor). atp-dependent cor catalysis requires interaction of the protein subcomplex (bchx)2 with the catalytic (bchy/bchz)2 protein to facilitate substrate reduction via two redox active iron-sulfur centers. the ternary cor enzyme holocomplex comprising subunits bchx, bchy, a ... | 2014 | 25422320 |
| respiratory kinetics of marine bacteria exposed to decreasing oxygen concentrations. | during aerobic respiration, microorganisms consume oxygen (o2) through the use of different types of terminal oxidases which have a wide range of affinities for o2. the km values for o2 of these enzymes have been determined to be in the range of 3 to 200 nmol liter(-1). in this study, we examined the time course of development of aerobic respiratory kinetics of four marine bacterial species (dinoroseobacter shibae, roseobacter denitrificans, idiomarina loihiensis, and marinobacter daepoensis) du ... | 2016 | 26682857 |
| n-terminal-oriented proteogenomics of the marine bacterium roseobacter denitrificans och114 using n-succinimidyloxycarbonylmethyl)tris(2,4,6-trimethoxyphenyl)phosphonium bromide (tmpp) labeling and diagonal chromatography. | given the ease of whole genome sequencing with next-generation sequencers, structural and functional gene annotation is now purely based on automated prediction. however, errors in gene structure are frequent, the correct determination of start codons being one of the main concerns. here, we combine protein n termini derivatization using (n-succinimidyloxycarbonylmethyl)tris(2,4,6-trimethoxyphenyl)phosphonium bromide (tmpp ac-osu) as a labeling reagent with the combined fractional diagonal chrom ... | 2014 | 24536027 |
| open source approaches to establishing roseobacter clade bacteria as synthetic biology chassis for biogeoengineering. | aim. the nascent field of bio-geoengineering stands to benefit from synthetic biologists' efforts to standardise, and in so doing democratise, biomolecular research methods. roseobacter clade bacteria comprise 15-20% of oceanic bacterio-plankton communities, making them a prime candidate for establishment of synthetic biology chassis for bio-geoengineering activities such as bioremediation of oceanic waste plastic. developments such as the increasing affordability of dna synthesis and laboratory ... | 2016 | 27441104 |
| "you produce while i clean up", a strategy revealed by exoproteomics during synechococcus-roseobacter interactions. | most of the energy that is introduced into the oceans by photosynthetic primary producers is in the form of organic matter that then sustains the rest of the food web, from micro to macro-organisms. however, it is the interactions between phototrophs and heterotrophs that are vital to maintaining the nutrient balance of marine microbiomes that ultimately feed these higher trophic levels. the primary produced organic matter is mostly remineralized by heterotrophic microorganisms but, because most ... | 2015 | 25728650 |
| complete genome sequence of the siphovirus roseophage rdjlφ 2 infecting roseobacter denitrificans och114. | rdjlφ2, a lytic phage that infects the marine bacterium roseobacter denitrificans och114, one of the model organisms of the roseobacter clade, was isolated. here we report the overall genome architecture of rdjlφ2. morphological and genome analysis revealed that rdjlφ2 is a siphovirus with a 63.5 kb genome that contains 76 putative gene products. | 2016 | 26541473 |
| ultrafast excited state processes in roseobacter denitrificans antennae: comparison of isolated complexes and native membranes. | roseobacter (rsb.) denitrificans is a marine aerobic anoxygenic photosynthetic purple bacterium with an unusually high-800 nm absorption band. ultrafast excited state processes have been intensively studied in the past in order to understand why the energy transfer efficiency between photosynthetic antennae approaches unity and recently it has been proved that the organization of the antennae proteins within the membranes plays an important role. thanks to the development of genetic manipulation ... | 2014 | 25361064 |
| magnetic immunoaffinity enrichment for selective capture and ms/ms analysis of n-terminal-tmpp-labeled peptides. | proteogenomics is the alliance of proteomics and genomics with the aim of better annotating structural genes based on experimental, protein-based data items established by tandem mass spectrometry. while, on average, more than one-tenth of protein n-termini are incorrectly annotated, there is a crucial need for methodological approaches to systematically establish the translational starts of polypeptides, and their maturations, such as n-terminal methionine processing and peptide signal excision ... | 2014 | 24313271 |
| identification of the genes encoding nitric oxide reductase in the aerobic photosynthetic bacterium roseobacter denitrificans och114. | a cytochrome bc-type complex of roseobacter denitrificans och114 was thought to be a novel cytochrome c oxidase. to determine its function, we deleted the genes encoding the complex. the mutant grew normally by aerobic respiration, but failed to grow by denitrification and lacked nitric oxide reductase activity, indicating that the physiological function of the gene product is nitric oxide reduction. | 2012 | 23047089 |
| pelagimonas varians gen. nov., sp. nov., isolated from the southern north sea. | a heterotrophic, gram-stain-negative, aerobic bacterium, designated strain sh4-1(t), was obtained from a seawater sample collected from the southern north sea during a phytoplankton bloom. the 16s rrna gene sequence comparison revealed affiliation to the roseobacter clade (class alphaproteobacteria) with sulfitobacter marinus sw-265(t) as the most closely related characterized strain, showing 97.2 % 16s rrna gene sequence similarity. calculation of phylogenetic trees based on 16s rrna gene seque ... | 2013 | 22611199 |
| co-cultures of pseudomonas aeruginosa and roseobacter denitrificans reveal shifts in gene expression levels compared to solo cultures. | consistent biosynthesis of desired secondary metabolites (sms) from pure microbial cultures is often unreliable. in a proof-of-principle study to induce sm gene expression and production, we describe mixed "co-culturing" conditions and monitoring of messages via quantitative real-time pcr (qpcr). gene expression of model bacterial strains (pseudomonas aeruginosa pao1 and roseobacter denitrificans och114) was analyzed in pure solo and mixed cocultures to infer the effects of interspecies interact ... | 2012 | 22566756 |
| diversity of purple nonsulfur bacteria in shrimp ponds with varying mercury levels. | this research aimed to study the diversity of purple nonsulfur bacteria (pnsb) and to investigate the effect of hg concentrations in shrimp ponds on pnsb diversity. amplification of the pufm gene was detected in 13 and 10 samples of water and sediment collected from 16 shrimp ponds in southern thailand. in addition to pnsb, other anoxygenic phototrophic bacteria (apb) were also observed; purple sulfur bacteria (psb) and aerobic anoxygenic phototrophic bacteria (aapb) although most of them could ... | 2016 | 27298580 |
| structure of the membrane-intrinsic nitric oxide reductase from roseobacter denitrificans. | membrane-intrinsic nitric oxide reductases (nors) are key components of bacterial denitrification pathways with a close evolutionary relationship to the cytochrome oxidase (cox) complex found in aerobic respiratory chains. a key distinction between cox and nor is the identity of the metal directly opposite heme b3 within the active site. in nor, this metal is iron (feb), whereas in cox, it is copper (cub). the purified nor of roseobacter denitrificans contains copper and has modest oxidase activ ... | 2016 | 27185533 |