| coexistence of multiple proteobacterial endosymbionts in the gills of the wood-boring bivalve lyrodus pedicellatus (bivalvia: teredinidae). | wood-boring bivalves of the family teredinidae (commonly called shipworms) are known to harbor dense populations of gram-negative bacteria within specialized cells (bacteriocytes) in their gills. these symbionts are thought to provide enzymes, e.g., cellulase and dinitrogenase, which assist the host in utilizing wood as a primary food source. a cellulolytic, dinitrogen-fixing bacterium, teredinibacter turnerae, has been isolated from the gill tissues of numerous teredinid bivalves and has been p ... | 2002 | 12450854 |
| teredinibacter turnerae gen. nov., sp. nov., a dinitrogen-fixing, cellulolytic, endosymbiotic gamma-proteobacterium isolated from the gills of wood-boring molluscs (bivalvia: teredinidae). | a cellulolytic, dinitrogen-fixing bacterium isolated from the gill tissue of a wood-boring mollusc (shipworm) lyrodus pedicellatus of the bivalve family teredinidae and 58 additional strains with similar properties, isolated from gills of 24 bivalve species representing 9 of 14 genera of teredinidae, are described. the cells are gram-negative, rigid, rods (0.4-0.6 x 3-6 microm) that bear a single polar flagellum. all isolates are capable of chemoheterotrophic growth in a simple mineral medium su ... | 2002 | 12508896 |
| celab, a multifunctional cellulase encoded by teredinibacter turnerae t7902t, a culturable symbiont isolated from the wood-boring marine bivalve lyrodus pedicellatus. | we characterized a multifunctional cellulase (celab) encoded by the endosymbiont teredinibacter turnerae t7902(t). celab contains two catalytic and two carbohydrate-binding domains, each separated by polyserine linker regions. celab binds cellulose and chitin, degrades multiple complex polysaccharides, and displays two catalytic activities, cellobiohydrolase (ec 3.2.1.91) and beta-1,4(3) endoglucanase (ec 3.2.1.4). | 2007 | 17933945 |
| the complete genome of teredinibacter turnerae t7901: an intracellular endosymbiont of marine wood-boring bivalves (shipworms). | here we report the complete genome sequence of teredinibacter turnerae t7901. t. turnerae is a marine gamma proteobacterium that occurs as an intracellular endosymbiont in the gills of wood-boring marine bivalves of the family teredinidae (shipworms). this species is the sole cultivated member of an endosymbiotic consortium thought to provide the host with enzymes, including cellulases and nitrogenase, critical for digestion of wood and supplementation of the host's nitrogen-deficient diet. t. t ... | 2009 | 19568419 |
| umboniibacter marinipuniceus gen. nov., sp. nov., a marine gammaproteobacterium isolated from the mollusc umbonium costatum from the sea of japan. | two bacterial strains, kmm 3891(t) and kmm 3892, were isolated from internal tissues of the marine mollusc umbonium costatum collected from the sea of japan. the novel isolates were gram-negative, aerobic, faint pink-reddish-pigmented, rod-shaped, non-motile, stenohaline and psychrotolerant bacteria that were unable to degrade most tested complex polysaccharides. polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. fatty acid analysis revealed c(17 ... | 2010 | 19654350 |
| a novel salt-tolerant endo-beta-1,4-glucanase cel5a in vibrio sp. g21 isolated from mangrove soil. | although cellulases have been isolated from various microorganisms, no functional cellulase gene has been reported in the vibrio genus until now. in this report, a novel endo-beta-1,4-glucanase gene, cel5a, 1,362 bp in length, was cloned from a newly isolated bacterium, vibrio sp. g21. the deduced protein of cel5a contains a catalytic domain of glycosyl hydrolase family 5 (gh5), followed by a cellulose binding domain (cbm2). the gh5 domain shows the highest sequence similarity (69%) to the bifun ... | 2010 | 20393708 |
| genetic modification of teredinibacter turnerae, an endosymbiont with biotechnological potential. | teredinibacter turnerae belongs to a group of biotechnologically relevant bacteria. gene transfer into t. turnerae was achieved by using pprobe'-gfp[asv] derived plasmids through conjugative mating with escherichia coli dh5alpha prk2073. transferred plasmids were stably maintained and t. turnerae could also act as a donor to transfer these mobilizable plasmids. constructs for both constitutive and iptg-inducible gene expression were obtained, representing new tools for gene overexpression in t. ... | 2010 | 20559015 |
| pseudoteredinibacter isoporae gen. nov., sp. nov., a marine bacterium, isolated from the reef-building coral isopora palifera. | a gram-negative, heterotrophic, marine bacterium, designated strain sw-11t, was isolated from the reef-building coral isopora palifera in kenting, taiwan. cells were rods and motile by a single polar flagellum. the optimum growth temperature was 30-35°c. cells grew best in ph 7.0-8.0 and 2-3%(w/v) nacl. the 16s rrna gene sequence analysis demonstrated that the strain sw-11t was unique, showing <93.5 % similarity with respect to the genera teredinibacter (maximum of 93.2 % similarity) and sacchar ... | 2010 | 20833889 |
| physiological traits of the symbiotic bacterium teredinibacter turnerae isolated from the mangrove shipworm neoteredo reynei. | nutrition in the teredinidae family of wood-boring mollusks is sustained by cellulolytic/nitrogen fixing symbiotic bacteria of the teredinibacter clade. the mangrove teredinidae neoteredo reynei is popularly used in the treatment of infectious diseases in the north of brazil. in the present work, the symbionts of n. reynei, which are strictly confined to the host's gills, were conclusively identified as teredinibacter turnerae. symbiont variants obtained in vitro were able to grow using casein a ... | 2009 | 21637522 |
| simiduia agarivorans gen. nov., sp. nov., a marine, agarolytic bacterium isolated from shallow coastal water from keelung, taiwan. | a gram-negative, heterotrophic, agarolytic, marine bacterium, designated strain sa1t, was isolated from a seawater sample collected in the shallow coastal region of keelung, taiwan. cells were straight to slightly curved rods. nearly all of the cells were non-motile and non-flagellated during the exponential phase of growth in broth cultures; a few cells (<1 %) were motile and were considered to have monotrichous flagella. the isolate required nacl for growth and grew optimally at 30-35 degrees ... | 2008 | 18398190 |
| turnerbactin, a novel triscatecholate siderophore from the shipworm endosymbiont teredinibacter turnerae t7901. | shipworms are marine bivalve mollusks (family teredinidae) that use wood for shelter and food. they harbor a group of closely related, yet phylogenetically distinct, bacterial endosymbionts in bacteriocytes located in the gills. this endosymbiotic community is believed to support the host's nutrition in multiple ways, through the production of cellulolytic enzymes and the fixation of nitrogen. the genome of the shipworm endosymbiont teredinibacter turnerae t7901 was recently sequenced and in add ... | 2013 | 24146831 |
| bacterial genome mining of enzymatic tools for alkyne biosynthesis. | the alkyne is an important functionality widely used in material science, pharmaceutical science, and chemical biology, but the importance of this functionality is contrasted by the very limited number of enzymes known to be involved in alkyne biosynthesis. we recently reported the first known carrier protein-dependent pathway for terminal alkyne formation, and in silico analysis suggested that this mechanism could be widespread in bacteria. in this paper, we screened additional homologous gene ... | 2015 | 26441143 |
| boronated tartrolon antibiotic produced by symbiotic cellulose-degrading bacteria in shipworm gills. | shipworms are marine wood-boring bivalve mollusks (family teredinidae) that harbor a community of closely related gammaproteobacteria as intracellular endosymbionts in their gills. these symbionts have been proposed to assist the shipworm host in cellulose digestion and have been shown to play a role in nitrogen fixation. the genome of one strain of teredinibacter turnerae, the first shipworm symbiont to be cultivated, was sequenced, revealing potential as a rich source of polyketides and nonrib ... | 2013 | 23288898 |
| identification and characterization of cellobiose 2-epimerases from various aerobes. | cellobiose 2-epimerase (ce), found mainly in anaerobes, reversibly converts d-glucose residues at the reducing end of β-1,4-linked oligosaccharides to d-mannose residues. in this study, we characterized ce-like proteins from various aerobes (flavobacterium johnsoniae nbrc 14942, pedobacter heparinus nbrc 12017, dyadobacter fermentans atcc 700827, herpetosiphon aurantiacus atcc 23779, saccharophagus degradans atcc 43961, spirosoma linguale atcc 33905, and teredinibacter turnerae atcc 39867), beca ... | 2013 | 23291764 |
| genetic differentiation among isolates of teredinibacter turnerae, a widely occurring intracellular endosymbiont of shipworms. | teredinibacter turnerae is a cultivable intracellular endosymbiont of xylotrophic (woodfeeding)bivalves of the family teredinidae (shipworms). although t. turnerae has been isolated from many shipworm taxa collected in many locations, no systematic effort has been made to explore genetic diversity within this symbiont species across the taxonomic and geographical range of its hosts. the mode of symbiont transmission is unknown. here, we examine sequence diversity in fragments of six genes (16s r ... | 2014 | 24765662 |
| extensive variation in intracellular symbiont community composition among members of a single population of the wood-boring bivalve lyrodus pedicellatus (bivalvia: teredinidae). | shipworms (wood-boring bivalves of the family teredinidae) harbor in their gills intracellular bacterial symbionts thought to produce enzymes that enable the host to consume cellulose as its primary carbon source. recently, it was demonstrated that multiple genetically distinct symbiont populations coexist within one shipworm species, lyrodus pedicellatus. here we explore the extent to which symbiont communities vary among individuals of this species by quantitatively examining the diversity, ab ... | 2006 | 16391072 |
| saccharophagus degradans gen. nov., sp. nov., a versatile marine degrader of complex polysaccharides. | gammaproteobacteria belonging and related to the genus microbulbifer are an emerging group of complex carbohydrate-degrading marine bacteria. previously, all of the representatives were placed within microbulbifer or were unclassified. recently, a new genus, teredinibacter, represented by a single species, teredinibacter turnerae, was formed to include an endosymbiotic branch of these organisms. in this study, based on 16s rrna gene sequence similarity and phenotypic analyses, a new genus, sacch ... | 2005 | 16014479 |
| maricurvus nonylphenolicus gen. nov., sp. nov., a nonylphenol-degrading bacterium isolated from seawater. | a novel aerobic, gram-negative bacterial strain, designated ku41e(t) , which degrades p-n-nonylphenol, was isolated from seawater obtained from the coastal region of ishigaki island, japan. cells are motile, curved rods with a single polar flagellum. strain ku41e(t) grew at 20-35 °c, ph 7.0-8.0, in the presence of 1.0-4.0% nacl. the predominant respiratory lipoquinone was ubiquinone-8, and the major cellular fatty acids were summed feature 3 (c(15:0) iso 2oh and/or c(16:1) ω7c, 28.4%), c(18:1 ... | 2011 | 22133150 |