| new compatible solutes related to di-myo-inositol-phosphate in members of the order thermotogales. | the accumulation of intracellular organic solutes was examined in six species of the order thermotogales by nuclear magnetic resonance spectroscopy. the newly discovered compounds di-2-o-beta-mannosyl-di-myo-inositol-1,1'(3,3')-phosphate and di-myo-inositol-1,3'-phosphate were identified in thermotoga maritima and thermotoga neapolitana. in the latter species, at the optimum temperature and salinity the organic solute pool was composed of di-myo-inositol-1,1'(3,3')-phosphate, beta-glutamate, and ... | 1996 | 8824608 |
| thermosipho melanesiensis sp. nov., a new thermophilic anaerobic bacterium belonging to the order thermotogales, isolated from deep-sea hydrothermal vents in the southwestern pacific ocean. | a new thermophilic, anaerobic rod-shaped bacterium, strain bi429t was isolated from the gills of a deep-sea vent hydrothermal mussel, bathymodiolus brevior, from the lau basin (southwestern pacific ocean). phenotypically, this isolate exhibited characteristics similar to those described for members of the order thermotogales. this organism was identified as a member of the genus thermosipho on the basis of the presence of the typical outer sheath-like structure (toga), its 16s rrna sequence, and ... | 1997 | 9336917 |
| estimation of genome sizes of hyperthermophiles. | genomes of various hyperthermophilic and extremely thermophilic prokaryotes were analyzed with respect to size, physical organization, and 16s rdna copy number. our results show that all the genomes are circular, and they are in the size range of 1.6-1.8 mb for pyrodictium abyssi, methanococcus igneus, pyrobaculum aerophilum, archaeoglobus fulgidus, archaeoglobus lithotrophicus, and archaeoglobus profundus (the two bacteria fervidobacterium islandicum and thermosipho africanus possess genomes of ... | 1998 | 9672684 |
| thermosipho japonicus sp. nov., an extremely thermophilic bacterium isolated from a deep-sea hydrothermal vent in japan. | a novel barophilic, extremely thermophilic bacterium was isolated from a deep-sea hydrothermal vent chimney at the iheya basin, in the okinawa area, japan. the cells were found to be rod shaped and surrounded by a sheath-like outer structure; the organism did not possess flagella and was not motile. growth was observed between 45 degrees and 80 degrees c (optimum, 72 degrees c, 45 min doubling time), ph 5.3 and 9.3 (optimum, ph 7.2-7.6), 6.6 and 79g/l sea salts (optimum, 40g/l), and 0.1 and 60mp ... | 2000 | 10741832 |
| polyamines of the thermophilic eubacteria belonging to the genera thermosipho, thermaerobacter and caldicellulosiruptor. | cellular polyamines of four new thermophiles located in three early branched eubacterial clades, were investigated for the chemotaxonomic significance of polyamine distribution profiles. the thermophilic anaerobic thermosipho japonicus, belonging to the order thermotogales, contained norspermidine, norspermine and thermospermine in addition to spermidine and spermine. the polyamine profile was identical to the polyamine composition of thermotoga, fervidobacterium and petrotoga species of the ord ... | 2001 | 11327112 |
| thermosipho geolei sp. nov., a thermophilic bacterium isolated from a continental petroleum reservoir in western siberia. | three strictly anaerobic, thermophilic bacteria (sl31t, sl30 and mlm39636) were isolated from a deep continental oil reservoir in western siberia (russia). following the mid-exponential phase of growth, the non-motile rod-shaped organisms were surrounded by a sheath-like structure. as dna-dna hybridizations showed that these strains were highly related genomically, only strain sl31t was studied in detail. the temperature range for growth of strain sl31t was between 45 and 75 degrees c, with opti ... | 2001 | 11491329 |
| radioisotopic, culture-based, and oligonucleotide microchip analyses of thermophilic microbial communities in a continental high-temperature petroleum reservoir. | activity measurements by radioisotopic methods and cultural and molecular approaches were used in parallel to investigate the microbial biodiversity and its physiological potential in formation waters of the samotlor high-temperature oil reservoir (western siberia, russia). sulfate reduction with rates not exceeding 20 nmol of h(2)s liter(-1) day(-1) occurred at 60 and 80 degrees c. in upper horizons (ab, a, and b), methanogenesis (lithotrophic and/or acetoclastic) was detected only in wells in ... | 2003 | 14532074 |
| order-specific 16s rrna-targeted oligonucleotide probes for (hyper)thermophilic archaea and bacteria. | new oligonucleotide probes were designed and evaluated for application in fluorescence in situ hybridization (fish) studies on (hyper)thermophilic microbial communities--arglo32, tcoc164, and aqui1197 target the 16s rrna of archaeoglobales, thermococcales, and aquificales, respectively. both sequence information and experimental evaluation showed high coverage and specificity of all three probes. the signal intensity of aqui1197 was improved by addition of a newly designed, unlabeled "helper" ol ... | 2004 | 15168171 |
| h(2) production and carbon utilization by thermotoga neapolitana under anaerobic and microaerobic growth conditions. | h(2) production by petrotoga miotherma, thermosipho africanus, thermotoga elfii, fervidobacterium pennavorans, and thermotoga neapolitana was compared under microaerobic conditions. contrary to these previously reported strains being strict anaerobes, all tested strains grew and produced h(2) in the presence of micromolar levels of o(2). t. neapolitana showed the highest h(2) production under these conditions. microscopic counting techniques were used to determine growth curves and doubling time ... | 2004 | 15289678 |
| hydrolysis of the amyloid prion protein and nonpathogenic meat and bone meal by anaerobic thermophilic prokaryotes and streptomyces subspecies. | transmissible spongiform encephalopathies are caused by accumulation of highly resistant misfolded amyloid prion protein prpres and can be initiated by penetration of such pathogen molecules from infected tissue to intact organism. decontamination of animal meal containing amyloid prion protein is proposed thanks to the use of proteolytic enzymes secreted by thermophilic bacteria thermoanaerobacter, thermosipho, and thermococcus subsp. and mesophilic soil bacteria streptomyces subsp. keratins al ... | 2004 | 15453713 |
| thermosipho atlanticus sp. nov., a novel member of the thermotogales isolated from a mid-atlantic ridge hydrothermal vent. | a novel anaerobic, thermophilic and heterotrophic bacterium, designated strain dv1140(t), was isolated from a deep-sea hydrothermal vent sample from the mid-atlantic ridge. the cells were non-motile straight rods, 1.8 microm long and 0.4 microm wide, surrounded by an outer sheath-like structure (toga). they grew at 45-80 degrees c (optimum 65 degrees c), ph 5.0-9.0 (optimum ph 6.0) and at sea salt concentrations of 20-60 g l(-1) (optimum 30 g l(-1)). strain dv1140(t) was able to ferment yeast ex ... | 2004 | 15545417 |
| thiosulfate reduction, an important physiological feature shared by members of the order thermotogales. | several members of the order thermotogales in the domain bacteria, viz., thermotoga neapolitana, thermotoga maritima, thermosipho africanus, fervidobacterium islandicum, and thermotoga strain sebr 2665, an isolate from an oil well, reduced thiosulfate to sulfide. this reductive process enhanced cellular yields and growth rates of all the members but was more significant with the two hyperthermophiles t. neapolitana and t. maritima. this is the first report of such an occurrence in this group of ... | 1995 | 16535035 |
| group-specific 16s rrna-targeted oligonucleotide probes to identify thermophilic bacteria in marine hydrothermal vents. | four 16s rrna-targeted oligonucleotide probes were designed for the detection of thermophilic members of the domain bacteria known to thrive in marine hydrothermal systems. we developed and characterized probes encompassing most of the thermophilic members of the genus bacillus, most species of the genus thermus, the genera thermotoga and thermosipho, and the aquificales order. the temperature of dissociation of each probe was determined. probe specificities to the target groups were demonstrate ... | 1997 | 16535717 |
| continuous enrichment cultures: insights into prokaryotic diversity and metabolic interactions in deep-sea vent chimneys. | the prokaryotic diversity of culturable thermophilic communities of deep-sea hydrothermal chimneys was analysed using a continuous enrichment culture performed in a gas-lift bioreactor, and compared to classical batch enrichment cultures in vials. cultures were conducted at 60 degrees c and ph 6.5 using a complex medium containing carbohydrates, peptides and sulphur, and inoculated with a sample of a hydrothermal black chimney collected at the rainbow field, mid-atlantic ridge, at 2,275 m depth. ... | 2007 | 17576518 |
| microbial community structure analysis of produced water from a high-temperature north sea oil-field. | molecular and culture-based methods were used to investigate the microbial diversity in produced water obtained from the high-temperature troll oil formation in the north sea. 16s rrna gene libraries were generated from total community dna, using universal archaeal or bacterial oligonucleotide primer sets. sequence analysis of 88 clones in the bacterial library indicated that they originated from members of firmicutes (48 sequences), bacteroidetes (17 sequences), delta-proteobacteria (15 sequenc ... | 2008 | 17588160 |
| structural characterization of diabolic acid-based tetraester, tetraether and mixed ether/ester, membrane-spanning lipids of bacteria from the order thermotogales. | the distribution of core lipids in the membranes of nine different species of the order thermotogales, one of the early and deep branching lineages in the bacteria, were examined by hplc/ms and demonstrated to consist of membrane-spanning diglycerol lipids comprised of diabolic acid-derived alkyl moieties. in the thermotoga species the core membrane lipids are characterized by the presence of both ester and ether bonds, whereas in the phylogenetically more distinct thermosipho and fervidobacteri ... | 2007 | 17643227 |
| evolution of mal abc transporter operons in the thermococcales and thermotogales. | the mal genes that encode maltose transporters have undergone extensive lateral transfer among ancestors of the archaea thermococcus litoralis and pyrococcus furiosus. bacterial hyperthermophiles of the order thermotogales live among these archaea and so may have shared in these transfers. the genome sequence of thermotoga maritima bears evidence of extensive acquisition of archaeal genes, so its ancestors clearly had the capacity to do so. we examined deep phylogenetic relationships among the m ... | 2008 | 18197971 |
| the genome of thermosipho africanus tcf52b: lateral genetic connections to the firmicutes and archaea. | lateral gene transfers (lgt) (also called horizontal gene transfers) have been a major force shaping the thermosipho africanus tcf52b genome, whose sequence we describe here. firmicutes emerge as the principal lgt partner. twenty-six percent of phylogenetic trees suggest lgt with this group, while 13% of the open reading frames indicate lgt with archaea. | 2009 | 19124572 |
| on the chimeric nature, thermophilic origin, and phylogenetic placement of the thermotogales. | since publication of the first thermotogales genome, thermotoga maritima strain msb8, single- and multi-gene analyses have disagreed on the phylogenetic position of this order of bacteria. here we present the genome sequences of 4 additional members of the thermotogales (tt. petrophila, tt. lettingae, thermosipho melanesiensis, and fervidobacterium nodosum) and a comprehensive comparative analysis including the original t. maritima genome. while ribosomal protein genes strongly place thermotogal ... | 2009 | 19307556 |
| thermosipho affectus sp. nov., a thermophilic, anaerobic, cellulolytic bacterium isolated from a mid-atlantic ridge hydrothermal vent. | a novel obligately anaerobic, extremely thermophilic, organotrophic bacterium, strain ik275mar(t), was isolated from a mid-atlantic ridge deep-sea hydrothermal vent. cells were rods surrounded by a sheath-like structure (toga), 0.4-0.9 µm in width and 1.2-6.0 µm in length. strain ik275mar(t) grew at 37-75 °c, ph 5.6-8.2 and at nacl concentrations of 10-55 g l(-1). under optimum conditions (70 °c, ph 6.6, nacl 20 g l(-1)), doubling time was 32 min. the isolate was able to ferment carbohydrates in ... | 2011 | 20562244 |
| thermosipho globiformans sp. nov., an anaerobic thermophilic bacterium that transforms to a multicellular spheroid with a defect in peptidoglycan formation. | an anaerobic rod-shaped thermophile was isolated from a hydrothermal vent at suiyo seamount, izu-bonin arc, western pacific ocean, and was named mn14(t). the rods were gram-negative, nonmotile without flagella, 2-4 microm long and 0.5 microm wide, and divided by binary fission in the mid-exponential phase. the cells were surrounded by a sheath-like structure (toga) and occurred singly or in chains. spheroids containing multiple cells were observed not only in the stationary phase as observed in ... | 2010 | 20693358 |
| phylogeny and molecular signatures for the phylum thermotogae and its subgroups. | thermotogae species are currently identified mainly on the basis of their unique toga and distinct branching in the rrna and other phylogenetic trees. no biochemical or molecular markers are known that clearly distinguish the species from this phylum from all other bacteria. the taxonomic/evolutionary relationships within this phylum, which consists of a single family, are also unclear. we report detailed phylogenetic analyses on thermotogae species based on concatenated sequences for many ribos ... | 2011 | 21503713 |