| phylogeny of helicobacter felis sp. nov., helicobacter mustelae, and related bacteria. | strain cs1t (t = type strain) is a gram-negative, microaerophilic, urease-positive, spiral-shaped bacterium that was isolated from the gastric mucosa of a cat. additional strains which possessed biochemical and ultrastructural characteristics similar to those of strain cs1t were isolated from the gastric mucosa of cats and dogs. the guanine-plus-cytosine content of the dna of strain cs1t was 42.5 mol%. the 16s rrna sequences of strain cs1t, strain ds3 (a spiral-shaped isolate from a dog), and he ... | 1991 | 1704791 |
| revision of campylobacter, helicobacter, and wolinella taxonomy: emendation of generic descriptions and proposal of arcobacter gen. nov. | hybridization experiments were carried out between dnas from more than 70 strains of campylobacter spp. and related taxa and either 3h-labeled 23s rrnas from reference strains belonging to campylobacter fetus, campylobacter concisus, campylobacter sputorum, campylobacter coli, and campylobacter nitrofigilis, an unnamed campylobacter sp. strain, and a wolinella succinogenes strain or 3h- or 14c-labeled 23s rrnas from 13 gram-negative reference strains. an immunotyping analysis of 130 antigens ver ... | 1991 | 1704793 |
| helicobacter muridarum sp. nov., a microaerophilic helical bacterium with a novel ultrastructure isolated from the intestinal mucosa of rodents. | helical organisms with novel ultrastructural characteristics were isolated from the intestinal mucosa of rats and mice. these bacteria were characterized by the presence of 9 to 11 periplasmic fibers which appeared as concentric helical ridges on the surface of each cell. the cells were motile with a rapid corkscrewlike motion and had bipolar tufts of 10 to 14 sheathed flagella. the bacteria were microaerophilic, nutritionally fastidious, and physiologically similar to helicobacter species and w ... | 1992 | 1736969 |
| isolation and characterization of "flexispira rappini" from laboratory mice. | a bacterium with an unusual ultrastructure and possessing a fusiform protoplasmic cylinder, spiral periplasmic fibers, and bipolar tufts of sheathed flagella was identified in the intestinal mucosae of laboratory mice. the organism was cultured under microaerophilic conditions and was found to rapidly hydrolyze urea. on the basis of 16s rrna gene sequence analysis, the organism was shown to be "flexispira rappini." "f. rappini" is closely related to members of the genus helicobacter and has been ... | 1993 | 7504685 |
| arcobacter-specific and arcobacter butzleri-specific 16s rrna-based dna probes. | the genus arcobacter encompasses gram-negative, aerotolerant, spiral-shaped bacteria formerly designated campylobacter cryaerophila. two genus-specific 16s rrna-based oligonucleotide dna probes (23-mer and 27-mer) were developed. the probes hybridized with strains of arcobacter butzleri (n = 58), arcobacter cryaerophilus (n = 19), and arcobacter skirrowii (n = 17). the probes did not cross-react with any of the reference strains of campylobacter, helicobacter, including "flexispira rappini," or ... | 1995 | 7545177 |
| flexispira rappini suspected in ovine abortion. | | 1994 | 8073597 |
| helicobacter acinonyx sp. nov., isolated from cheetahs with gastritis. | four strains of a novel helicobacter species were isolated from the stomachs of cheetahs (acinonyx jubilatus) with gastritis. these isolates were phenotypically similar to helicobacter pylori. the isolates were gram-negative, spiral bacteria which grew under microaerophilic conditions at 37 degrees c, but not at 25 or 42 degrees c, and produced urease, catalase, oxidase, alkaline phosphatase, and gamma-glutamyl transpeptidase. the isolates did not ferment glucose, mannitol, inositol, sorbitol, r ... | 1993 | 8379970 |
| lipid profile of helicobacter spp.: presence of cholesteryl glucoside as a characteristic feature. | the lipid and fatty acid profiles of eight helicobacter spp. (h. nemestrinae, h. acinonyx, h. canis, helicobacter sp. strain clo-3, "h. rappini" [flexispira rappini], h. pametensis, helicobacter sp. strain bird-b, and helicobacter sp. strain bird-c) and the fatty acid profiles of five additional species (h. pylori, h. felis, h. muridarum, h. mustelae, and h. fennelliae) were analyzed and compared. a heterologous fatty acid profile was observed among the helicobacter spp., and on that basis the s ... | 1996 | 8606185 |
| identification of murine helicobacters by pcr and restriction enzyme analyses. | three murine helicobacter species have recently been identified: helicobacter hepaticus, helicobacter muridarum, and helicobacter bilis. infections with h. hepaticus and h. bilis have been associated with hepatitis and hepatic neoplasia. in this study, oligonucleotide primers were designed from regions of the 16s rrna gene that are conserved among members of the helicobacter genus. the assay amplified the expected 374-bp product from all three rodent helicobacter species and was able to detect a ... | 1996 | 8815113 |
| prevalence and varieties of helicobacter species in dogs from random sources and pet dogs: animal and public health implications. | gastric bacteria of a variety of ultrastructural morphologies have been identified in or isolated from domestic carnivores, but their prevalence in different populations of animals and their clinical significance are still unknown. the purposes of this study were (i) to evaluate the prevalence and morphologic types of gastric bacterial in three different populations of dogs; (ii) to determine which of the organisms were culturable, and if the cultured organisms were morphologically similar to th ... | 1996 | 8940465 |
| hepatic helicobacter species identified in bile and gallbladder tissue from chileans with chronic cholecystitis. | cancer of the gallbladder is the number one cause of cancer mortality in chilean women. incidence rates for this tumor vary widely on a worldwide basis, being approximately 30 times higher in high-risk than in low-risk populations, suggesting that environmental factors such as infectious microorganisms, carcinogens, and nutrition play a role in its pathogenesis. because several helicobacter sp. colonize the livers of animals and induce hepatitis, the aim of this study was to determine whether he ... | 1998 | 9516396 |
| "flexispira rappini" bacteremia in a child with pneumonia. | we describe a case of "flexispira rappini" bacteremia from a 9-year-old girl who presented with a 5-day history of fever, productive cough, and malaise. a chest x-ray result was compatible with right middle lobe pneumonia. blood culture grew a gram-negative spiral fusiform bacterium 2 days after the inoculation. biochemical tests showed the organism to be catalase negative, oxidase positive, sodium hippurate hydrolysis negative, and urea hydrolysis negative. 16s rrna gene sequencing identified t ... | 1998 | 9620399 |
| isolation and identification of helicobacter spp. from canine and feline gastric mucosa. | it is known that virtually all healthy adult dogs and cats harbor spiral helicobacters in their gastric mucosa. three species, helicobacter felis, helicobacter bizzozeronii, and helicobacter salomonis have been isolated in vitro from the gastric mucosa of these animals. the aims of this study were to evaluate the efficacy of an isolation method for canine and feline gastric helicobacters that has been developed at the university of helsinki; to estimate the prevalence and distribution of these t ... | 1998 | 9758832 |
| helicobacter colonization of the biliary tree: commensal, pathogen, or spurious finding? | the authors hypothesized that helicobacter species may be present in the bile and gallbladder wall of patients with chronic cholecystitis who live in a region with a high prevalence of gallbladder cancer. they attempted to identify such species by obtaining both bile and resected gallbladder tissue from 46 patients who underwent cholecystectomy. tissue specimens were stained with hematoxylin and eosin as well as other stains used specifically for the identification of helicobacter species, and c ... | 1998 | 9772078 |
| pathology, diagnosis and epidemiology of the rodent helicobacter infection. | since the first isolation of helicobacter pylori from humans in 1983, 18 helicobacter species have been identified during the last decade in domestic and laboratory animals. several helicobacter species have been isolated from the gastrointestinal tracts of various mammalian species and birds. helicobacter hepaticus, h. muridarum, h. bilis, h. rodentium and flexispira rappini have been isolated from mice. among these species, only h. hepaticos has been clearly recognized as a pathogen. indeed, i ... | 1999 | 10099028 |
| recurrent "flexispira rappini" bacteremia in an adult patient undergoing hemodialysis: case report. | a blood culture from a 65-year-old febrile man undergoing hemodialysis revealed, 5 days after inoculation, an unusual gram-negative fusiform rod with darting motility. during another episode of fever 21 days later, this campylobacter-like organism was again recovered from three blood cultures and subcultured under an h2-enriched microaerobic atmosphere. the organism was catalase negative and oxidase positive and hydrolyzed urea rapidly. sodium dodecyl sulfate-polyacrylamide gel electrophoresis a ... | 1999 | 10203478 |
| recurrent bacteremia caused by a "flexispira"-like organism in a patient with x-linked (bruton's) agammaglobulinemia. | helicobacter spp., except for helicobacter cinaedi, have only rarely been reported in cases of septicemia. a patient with x-linked (bruton's) agammaglobulinemia was found to have persistent sepsis with a helicobacter-like organism despite multiple courses of antibiotics. his periods of sepsis were associated with leg swelling thought to be consistent with cellulitis. the organism was fastidious and required a microaerophilic environment containing h(2) for growth. optimal growth was observed at ... | 1999 | 10405381 |
| occurrence and characterization of gastric helicobacter spp. in naturally infected dogs. | helicobacter-like organisms are frequently observed in the stomach of dogs but the relationship between these microorganisms and gastric pathology has not been clearly established. different species of helicobacters are known to be present in the canine stomach but their specific prevalence in naturally infected dogs is unknown. the aims of this study were to isolate and characterize helicobacters in canine gastric biopsies, to compare the commonly used tests for the identification of helicobact ... | 1999 | 10596807 |
| identification of a unique helicobacter species by 16s rrna gene analysis in an abdominal abscess from a patient with x-linked hypogammaglobulinemia. | a unique helicobacter species, mz640285, was isolated from a patient with x-linked hypogammaglobulinemia suffering from recurrent abdominal abscesses and was identified by 16s rrna gene sequence analysis. in the phylogenetic tree, the isolate fell into a cluster which included flexispira rappini, helicobacter bilis, and helicobacter sp. strain mainz. helicobacters are being increasingly recognized as pathogens in immunocompromised hosts. these fastidious bacteria are not easily cultured in the r ... | 2000 | 10878075 |
| current status of helicobacter contamination of laboratory mice, rats, gerbils, and house musk shrews in japan. | the current status of helicobacter contamination of laboratory mice, rats, gerbils, and house musk shrews from 47, 10, 4, and 3 colonies, respectively, in japan was studied. helicobacter was detected by reverse transcription (rt)-nested polymerase chain reaction (pcr) with helicobacter genus-specific primers by using feces obtained from the animals. h. hepaticus, h. muridarum, h. bilis, h. rodentium, "flexispira rappini", and "h. suncus" were identified with species-specific primers. other speci ... | 2000 | 10915200 |
| bacteremia and skin/bone infections in two patients with x-linked agammaglobulinemia caused by an unusual organism related to flexispira/helicobacter species. | two patients with bruton's x-linked agammaglobulinemia are described with bacteremia and skin/bone infection due to an organism which by 16s rrna gene sequence analysis was most closely related to "flexispira" rappini (and thus designated a flexispira-like organism, flo) and more distantly related to the helicobacter species. the organism required microaerobic conditions and, supplemental h(2) gas for growth and was reliably stained with acridine orange. in common with helicobacter cinaedi infec ... | 2000 | 11027452 |
| characterization of culture-derived spiral bacteria by 16s ribosomal rna gene sequence analysis. | broad range amplification and sequence analysis of the 16s ribosomal rna gene was used to identify three spiral-form organisms. the agents were identified as campylobacter fetus, "flexispira rappini", and borrelia burgdorferi, respectively, using either proprietary or public sequence databases. in each case, the rdna sequence showed 99-100% homology with known sequence data. sequence-based analysis for each isolate required only 2-3 days, whereas traditional means of identification took 8-12 day ... | 2001 | 11173192 |
| helicobacters of possible zoonotic origin: a review. | since the isolation of helicobacter pylori, many new helicobacter species have been identified from the gastrointestinal tract in humans and animals. in humans, a spiral organism different from h. pylori and provisionally named "helicobacter heilmannii", has been associated with gastritis, gastric ulceration and to a lesser degree, gastric cancer. in addition helicobacter cinaedi, helicobacter fennelliae, helicobacter pullorum and "flexispira rappini" have been isolated from cases of enteric dis ... | 2000 | 11233522 |
| helicobacter sp. flexispira bacteremia in an immunocompetent young adult. | a young immunocompetent patient was admitted for a febrile illness with malaise, arthralgias, painful leg swelling, and polyserositis. shortly prior to becoming ill, the patient had traveled to the northern african desert. the symptoms disappeared during treatment with antibiotics (doxycycline and ceftriaxone) but recurred twice after stopping therapy. a motile gram-negative fusiform rod was isolated from a blood culture taken on the first admission. analysis of the 16s rrna gene of the blood cu ... | 2001 | 11325980 |
| recurrent bacteremia and multifocal lower limb cellulitis due to helicobacter-like organisms in a patient with x-linked hypogammaglobulinemia. | we describe a 27-year-old man with x-linked (bruton's) hypogammaglobulinemia who presented during a 10-month period with recurrent fevers and multifocal lower-limb cellulitis associated with bacteremia due to helicobacter-like organisms ("flexispira rappini" and helicobacter canis). susceptible individuals may acquire infection of this type as a result of exposure to young dogs. | 2001 | 11595979 |
| [flexispira rappini bacteremia and common variable immuodeficiency]. | | 2005 | 15780900 |
| pyoderma gangrenosum-like ulcer in a patient with x-linked agammaglobulinemia: identification of helicobacter bilis by mass spectrometry analysis. | pyoderma gangrenosum-like ulcers and cellulitis of the lower extremities associated with recurrent fevers in patients with x-linked (bruton) agammaglobulinemia have been reported to be caused by helicobacter bilis (formerly classified as flexispira rappini and then helicobacter strain flexispira taxon 8). consistent themes in these reports are the difficulty in recovering this organism in blood and wound cultures and in maintaining isolates in vitro. we confirmed the presence of this organism in ... | 2010 | 20479300 |