| reduction of nitrosubstituted aromatic compounds by the halophilic anaerobic eubacteria haloanaerobium praevalens and sporohalobacter marismortui. | the moderately halophilic, obligately anaerobic eubacteria haloanaerobium praevalens dsm 2228 and sporohalobacter marismortui atcc 35420 are able to reduce a variety of nitrosubstituted aromatic compounds at a high rate to the corresponding amines. compounds degraded included nitrobenzene, o-nitrophenol, m-nitrophenol, p-nitrophenol, nitroanilines, 2,4-dinitrophenol, and 2,4-dinitroaniline. most of these compounds, when added at concentrations of 50 to 100 mg/liter, were completely transformed w ... | 1991 | 1669931 |
| the primary structure of the ribosomal a-protein (l12) from the halophilic eubacterium haloanaerobium praevalens. | the ribosomal a-protein, equivalent to the ribosomal protein l12 from escherichia coli, has been sequenced from the anaerobic halophilic eubacterium haloanaerobium praevalens (dsm 2228). the protein contains 122 amino acids, has a composition of asp6, asn2, thr2, ser6, glu22, pro2, gly13, ala19, val12, met4, ile5, leu11, phe3, lys14, arg1 and has a molecular weight of 12,691. the hydrophilicity profile was determined for this protein. a phylogenetic or cluster tree was calculated from computer a ... | 1987 | 3126821 |
| haloanaerobium salsugo sp. nov., a moderately halophilic, anaerobic bacterium from a subterranean brine. | a strictly anaerobic, moderately halophilic, gram-negative bacterium was isolated from a highly saline oil field brine. the bacterium was a non-spore-forming, nonmotile rod, appearing singly, in pairs, or occasionally as long chains, and measured 0.3 to 0.4 by 2.6 to 4 microns. the bacterium had a specific requirement for nacl and grew at nacl concentrations of between 6 and 24%, with optimal growth at 9% nacl. the isolate grew at temperatures of between 22 and 51 degrees c and ph values of betw ... | 1994 | 7520744 |
| haloanaerobium alcaliphilum sp. nov., an anaerobic moderate halophile from the sediments of great salt lake, utah. | a strictly anaerobic, moderately halophilic, gram-negative, rod-shaped bacterium was isolated from great salt lake, utah, sediments and designated gslst (t = type strain). strain gslst grew optimally at ph 6.7 to 7.0 but had a very broad ph range for growth (ph 5.8 to 10.0). the optimum temperature for growth was 37 degrees c, and no growth occurred at 15 or 55 degrees c. the optimum salt concentration for growth was 10%. strain gslst required yeast extract and trypticase peptone to ferment carb ... | 1995 | 7537063 |
| haloanaerobium lacusroseus sp. nov., an extremely halophilic fermentative bacterium from the sediments of a hypersaline lake. | a new extremely halophilic chemoorganotrophic bacterium (strain h200t [t = type strain]) was isolated from the hypersaline sediments of retba lake in senegal. this organism was a sluggishly motile, rod-shaped, non-spore-forming, gram-negative, obligate anaerobe that grew optimally at 40 degrees c in the presence of 180 to 200 g of nacl per liter. the dna base composition was 32 mol% guanine plus cytosine. the fermentation products from glucose were ethanol, acetate, h2, and co2. yeast extract wa ... | 1995 | 7547301 |
| reevaluating the classification of halobacteroides and haloanaerobacter species based on sequence comparisons of the 16s ribosomal rna gene. | the 16s rrna gene (rdna) sequence analysis of four halophilic anaerobes: halobacteroides halobius, h. lacunaris. haloanaerobacter (hb.) chitinovorans and h. acetoethylicus confirmed that they were all members of the family haloanaerobiaceae. h. lacunaris and h. halobius were found to be more closely related to each other and were distantly related to sporohalobacter lortetti and the members of the genera haloanaerobium and halothermothrix. these data are in agreement with their assignment to the ... | 1995 | 8593948 |
| haloanaerobium congolense sp. nov., an anaerobic, moderately halophilic, thiosulfate- and sulfur-reducing bacterium from an african oil field. | a strictly anaerobic, moderately halophilic, gram-negative, non-motile rod-shaped bacterium was isolated from an oil-well head sample of an offshore congolese oil field. the strain, designated sebr 4224t (t = type strain), grew optimally at 42 degrees c and ph 7.0 in a complex medium containing 10% nacl with a generation time of 2.5 h. strain sebr 4224t grew on a range of carbohydrates including fructose, galactose, d-glucose, maltose, d-mannose, d-ribose, sucrose, and trehalose. yeast extract a ... | 1997 | 9037768 |
| haloanaerobium kushneri sp. nov., an obligately halophilic, anaerobic bacterium from an oil brine. | three strains, designated vs-751t, vs-511 and vs-732, of a strictly anaerobic, moderately halophilic, gram-negative, rod-shaped bacterium were isolated from a highly saline (15-20%) brine from an oil reservoir in central oklahoma, usa. the optimal concentration of nacl for growth of these three strains was 2 m (12%), and the strains also grew in the presence of an additional 1 m mgcl2. the strains were mesophilic and grew at a ph range of 6-8. carbohydrates used by all three strains included glu ... | 1999 | 10425750 |
| strictly anaerobic halophiles isolated from canned swedish fermented herrings (surströmming). | strictly anaerobic halophiles were isolated from canned swedish fermented herrings (surströmming). all isolates were phenotypically uniform with some exceptions and were identified as the genus haloanaerobium and assigned to either haloanaerobium praevalens or haloanaerobiuim alcaliphilum. a comparative analysis of 16s rdna sequences revealed that the representative strain s-8 of the isolates was identical to that of haloanaerobium praevalens dsm 2228t. furthermore, this strain exhibited high le ... | 2000 | 10746577 |
| haloanaerobium fermentans sp. nov., a strictly anaerobic, fermentative halophile isolated from fermented puffer fish ovaries. | a strain of strictly anaerobic and moderately halophilic bacteria isolated from salted puffer fish ovaries was studied phenotypically, genotypically and phylogenetically. on the basis of its physiological and morphological characteristics, the new isolate is considered to be a member of the genus haloanaerobium. it is a motile, rod-shaped, non-spore-forming, gram-negative, obligate anaerobe that grows in the presence of 25% (w/v) nacl. the optimum salt concentration for growth is 10% (w/v). it g ... | 2000 | 10939669 |
| change of the names haloanaerobiales, haloanaerobiaceae and haloanaerobium to halanaerobiales, halanaerobiaceae and halanaerobium, respectively, and further nomenclatural changes within the order halanaerobiales. | a formal proposal is made to rename the order haloanaerobiales as halanaerobiales, the family haloanaerobiaceae as halanaerobiaceae, the genera haloanaerobium and haloanaerobacter as halanaerobium and halanaerobacter, respectively, and to change the specific epithets of halanaerobium acetoethylicum, halanaerobacter lacunaris, halocella cellulolytica and halanaerobium chitinovorans to acetethylicum, lacunarum, cellulosilytica and chitinivorans, respectively. these changes are required in view of ... | 2000 | 11156000 |
| microbial diversity of the brine-seawater interface of the kebrit deep, red sea, studied via 16s rrna gene sequences and cultivation methods. | the brine-seawater interface of the kebrit deep, northern red sea, was investigated for the presence of microorganisms using phylogenetic analysis combined with cultivation methods. under strictly anaerobic culture conditions, novel halophiles were isolated. the new rod-shaped isolates belong to the halophilic genus halanaerobium and are the first representatives of the genus obtained from deep-sea, anaerobic brine pools. within the genus halanaerobium, they represent new species which grow chem ... | 2001 | 11425725 |
| evidence of interspecies hydrogen transfer from glycerol in saline environments. | two halanaerobic bacteria--halanaerobium saccharolytica subsp. senegalense and halanaerobium sp. strain fr1h--produced acetate, h2, and co2 from glycerol fermentation, but the glycerol consumption rate was low. in contrast, in the presence of the moderately halophilic hydrogenotrophic sulfate-reducing bacterium, desulfohalobium retbaense, used as h2 scavenger in the coculture, glycerol oxidation by both halanaerobes significantly increased. cocultures of both halanaerobes with d. retbaense on gl ... | 2002 | 12013433 |
| the nomenclatural types of the orders acholeplasmatales, halanaerobiales, halobacteriales, methanobacteriales, methanococcales, methanomicrobiales, planctomycetales, prochlorales, sulfolobales, thermococcales, thermoproteales and verrucomicrobiales are the genera acholeplasma, halanaerobium, halobacterium, methanobacterium, methanococcus, methanomicrobium, planctomyces, prochloron, sulfolobus, thermococcus, thermoproteus and verrucomicrobium, respectively. opinion 79. | the judicial commission of the international committee on systematics of prokaryotes has corrected the nomenclatural types of 12 orders: acholeplasmatales, halanaerobiales, halobacteriales, methanobacteriales, methanococcales, methanomicrobiales, planctomycetales, prochlorales, sulfolobales, thermococcales, thermoproteales and verrucomicrobiales. | 2005 | 15653928 |
| rdla, a new gene encoding a rhodanese-like protein in halanaerobium congolense and other thiosulfate-reducing anaerobes. | the recently described anaerobic moderately halophilic bacterium halanaerobium congolense has been shown to reduce thiosulfate and sulfur-but not sulfate-into sulfide. when cultivated in the presence of thiosulfate as terminal electron acceptor, h. congolense possesses a highly active thiosulfate:cyanide sulfur-transferase activity (rhodanese-like enzyme). a gene library of h. congolense (dsm 11287t) was constructed, and a 3.1-kb sau3a dna that encompassed a thiosulfate:cyanide sulfur-transferas ... | 2005 | 16085393 |
| characterization of bacterial community associated to biofilms of corroded oil pipelines from the southeast of mexico. | microbial communities associated to biofilms promote corrosion of oil pipelines. the community structure of bacteria in the biofilm formed in oil pipelines is the basic knowledge to understand the complexity and mechanisms of metal corrosion. to assess bacterial diversity, biofilm samples were obtained from x52 steel coupons corroded after 40 days of exposure to normal operation and flow conditions. the biofilm samples were directly used to extract metagenomic dna, which was used as template to ... | 2006 | 16765858 |
| analysis of the composition of bacterial communities in oil reservoirs from a southern offshore brazilian basin. | the aim of this study was to characterize and compare the bacterial community structure of two distinct oil samples from a petroleum field in brazil by using both molecular, based on the construction of 16s rrna gene libraries, and cultivation methods. statistical comparisons of libraries based on amplified ribosomal dna restriction analysis (ardra) data revealed no significant differences between the communities recovered in the non-biodegraded (nbd) and highly biodegraded oils (hbd). blastn an ... | 2007 | 17072536 |
| hydrogen production from glycerol using halophilic fermentative bacteria. | glycerol-based hydrogen production by the halophilic bacteria halanaerobium saccharolyticum subspecies saccharolyticum and senegalensis was studied as batch experiments. the main metabolites of glycerol fermentation of both strains were hydrogen, carbon dioxide, and acetate. subspecies saccharolyticum also produced 1,3-propanediol (1,3-pd), butyrate, and ethanol. the highest hydrogen yields were achieved with 2.5g/l glycerol and 150g/l salt at ph 7.4 (subsp. saccharolyticum, yield 0.6mol/mol gly ... | 2010 | 20615687 |
| complete genome sequence of the haloalkaliphilic, hydrogen producing halanaerobium hydrogenoformans. | halanaerobium hydrogenoformans is an alkaliphilic bacterium capable of biohydrogen production at ph 11 and 7% (w/v) salt. we present the 2.6 mb genome sequence to provide insights into its physiology and potential for bioenergy applications. | 2011 | 21602336 |
| complete genome sequence of the extremely halophilic halanaerobium praevalens type strain (gsl). | halanaerobium praevalens zeikus et al. 1984 is the type species of the genus halanaerobium, which in turn is the type genus of the family halanaerobiaceae. the species is of interest because it is able to reduce a variety of nitro-substituted aromatic compounds at a high rate, and because of its ability to degrade organic pollutants. the strain is also of interest because it functions as a hydrolytic bacterium, fermenting complex organic matter and producing intermediary metabolites for other tr ... | 2011 | 21886858 |
| prokaryotic diversity in aran-bidgol salt lake, the largest hypersaline playa in iran. | prokaryotic diversity in aran-bidgol salt lake, a thalasohaline lake in iran, was studied by fluorescence in situ hybridization (fish), cultivation techniques, denaturing gradient gel electrophoresis (dgge) of pcr-amplified fragments of 16s rrna genes and 16s rrna gene clone library analysis. viable counts obtained (2.5-4×10(6) cells ml(-1)) were similar to total cell abundance in the lake determined by dapi direct count (3-4×10(7) cells ml(-1)). the proportion of bacteria to archaea in the comm ... | 2011 | 22185719 |
| 1,3-Propanediol production and tolerance of a halophilic fermentative bacterium, Halanaerobium saccharolyticum subsp. saccharolyticum. | 1,3-Propanediol (1,3-PD) is widely used in polymer industry in production of polyethers, polyesters and polyurethanes. In this article, a study on 1,3-PD production and tolerance of Halanaerobium saccharolyticum subsp. saccharolyticum is presented. 1,3-PD production was optimized for temperature, vitamin B(12) and acetate concentration. The highest 1,3-PD concentrations and yields (0.6mol/mol glycerol) were obtained at vitamin B(12) concentration 64µg/l and an inverse correlation between 1,3-PD ... | 2011 | 22085971 |
| sulfidogenesis in hypersaline chloride-sulfate lakes of kulunda steppe (altai, russia). | the activity and culturable diversity of sulfidogens were investigated in anoxic sediments of four hypersaline lakes with ph 7.6-8.2 in the kulunda steppe (altai, russia). sulfate reduction rates were low, varying from 0.1 to 6.0 nmol hs(-) /(cm(3) h) with a maximum in the top 10 cm layer. potential sulfidogenic rates with thiosulfate and sulfur as the e-acceptors were higher than with sulfate and were stimulated by formate, lactate, and acetate. sulfidogenesis was optimal at salt concentration ... | 2011 | 22092787 |