| a simple chromatographic procedure for the detection of cyclized archaebacterial glycerol-bisdiphytanyl-glycerol tetraether core lipids. | archaebacterial glycerol-bisdiphytanyl-glycerol tetraether core lipids containing from one to eight cyclopentane rings could be resolved from each other and from the parent uncyclized c40, c40 lipid by tlc. the core lipids of examples from the genera methanobacterium, methanobrevibacter, methanogenium and methanoplanus did not contain cyclized forms of glycerol-bisdiphytanyl-glycerol tetraethers, whereas the core lipids of methanosarcina barkeri contained glycerol-bisdiphytanyl-glycerol tetraeth ... | 1988 | 3151990 |
| 7-methylpterin derivatives in extracts of methanogens characterized by a relatively low methanopterin content. | cofactor extracts of five hydrogenotrophic methanogenic bacteria which contain relatively low amounts of methanopterin were screened for the presence of 7-methylpterin derivatives. extracts of methanospirillum hungatei, methanobrevibacter smithii and methanoplanus endosymbiosus were found to contain 7-methylpterin and methanopterin. these compounds were absent in extracts of methanogenium thermophilicum and methanogenium tatii. an unidentified methanopterin-like compound was detected in extracts ... | 1988 | 3255348 |
| cofactor contents of methanogenic bacteria reviewed. | the content of specific methanogenic cofactors was assessed for a range of hydrogenotrophic and methylotrophic methanogenic bacteria grown on different substrates using high performance liquid chromatography. in general, all methanogens were found to contain coenzyme f420 analogues, methanopterin (mpt) analogues and 5-hydroxybenzimidazolylcobamide (vitamin b12-hbi). in hydrogenotrophic methanogens of the genera methano-bacterium and methanobrevibacter, as a rule, coenzymes f420-2 and f420-3 as w ... | 1994 | 7916957 |
| improved strategy for presumptive identification of methanogens using 16s riboprinting. | the predicted 16s riboprint patterns of 10 restriction endonucleases for 26 diverse methanogens were compared to actual patterns produced on agarose gels. the observed patterns corroborated the expected riboprints. our analyses confirmed that the endonuclease haeiii gave the best results generating 15 different riboprint sets. six of these 15 riboprints represented more than one strain. of these, three riboprint sets were further differentiated: methanomicrobium mobile, methanolacinia paynteri, ... | 2003 | 14529955 |
| evaluation of methanogenic strains and their ability to endure aeration and water stress. | during periods of drainage, both water stress and oxygen can cause damage to indigenous methanogens. in the present study, we evaluated the tolerance of seven methanogenic strains (methanobrevibacter arboriphilicus, methanobacterium formicicum, methanococcus vannielii, methanospirillum hungatei, methanoculleus olentangyi, methanoplanus limicola, and methanosarcina mazei) to long-term exposure to air/nitrogen and drying. we found that these methanogenic strains except for m. limicola and m. olent ... | 2008 | 17990030 |
| methanogen population in a marine biofilm corrosive to mild steel. | this study was conducted to analyze the methanogen population in a corrosive marine biofilm based on 16s rdna analysis, using a pcr-cloning-sequencing approach. there were 80 methanogen clones developed from the pcr-amplified dna extracted from the biofilm on the mild steel surface. all clones were categorized into one of five operational taxonomy units (otus). two otus (comprising 57 clones) were affiliated with the acetotrophic methanosaeta genus; the remaining three otus (23 clones) were affi ... | 2003 | 12879307 |
| [use of the level of g+c n dna for studying the molecular phylogeny o f methanogenic archaebacteria]. | a new method for theoretical analysis of the molecular phylogeny of bacteria, successfully applied earlier to nitrifying bacteria, was used to study the molecular phylogeny of methanogenic archaebacteria. the group studied included methanococcus igneus, methanococcus vannielii, methanothermus fervidus, methanolobus tindarius, methanobacterium formicicum, methanosarcina barkeri, methanobacterium thermoformicicum, methanoplanus limicola, methanospirillum hungatei, and methanobacterium thermoautotr ... | 1999 | 10624685 |
| methanoplanus petrolearius sp. nov., a novel methanogenic bacterium from an oil-producing well. | a disc-shaped methanogenic bacterium designated strain sebr 4847t (t = type strain) was isolated from a sample collected from an african offshore oil field. strain sebr 4847t was non-motile, had a g + c content of 50 mol% and produced methane from h2 + co2, formate, and co2 + propanol. strain sebr 4847t grew optimally at 37 degrees c; no growth was observed at 25 degrees c or 45 degrees c. it grew in the presence of up to 50 g/l nacl; 10-30 g/l was required for optimal growth. the optimum ph for ... | 1997 | 9037763 |
| systematic and morphological diversity of endosymbiotic methanogens in anaerobic ciliates. | the identities and taxonomic diversity of the endosymbiotic methanogens from the anaerobic protozoa metopus contortus, metopus striatus, metopus palaeformis, trimyema sp. and pelomyxa palustris were determined by comparative analysis of their 16s ribosomal rna sequences. fluorescent oligonucleotide probes were designed to bind to the symbiont rrna sequences and to provide direct visual evidence of their origins from methanogenic archaea contained within the host cells. confocal microscopy was us ... | 1993 | 8085789 |
| complete genome sequence of methanoplanus petrolearius type strain (sebr 4847). | methanoplanus petrolearius ollivier et al. 1998 is the type strain of the genus methanoplanus. the strain was originally isolated from an offshore oil field from the gulf of guinea. members of the genus methanoplanus are of interest because they play an important role in the carbon cycle and also because of their significant contribution to the global warming by methane emission in the atmosphere. like other archaea of the family methanomicrobiales, the members of the genus methanoplanus are abl ... | 2010 | 21304750 |
| salinity constraints on subsurface archaeal diversity and methanogenesis in sedimentary rock rich in organic matter. | the diversity of microorganisms active within sedimentary rocks provides important controls on the geochemistry of many subsurface environments. in particular, biodegradation of organic matter in sedimentary rocks contributes to the biogeochemical cycling of carbon and other elements and strongly impacts the recovery and quality of fossil fuel resources. in this study, archaeal diversity was investigated along a salinity gradient spanning 8 to 3,490 mm cl(-) in a subsurface shale rich in ch(4) d ... | 2007 | 17468287 |
| identification of para-cresol as a growth factor for methanoplanus endosymbiosus. | growth of the methanogenic bacterium methanoplanus endosymbiosus is dependent on the presence of ruminal fluid. ruminal fluid could be replaced by the eluate of a rumen-derived anaerobic digester. from the eluate of the digester, a growth-stimulatory component was purified and identified as p-cresol. authentic p-cresol supported a half-maximal growth rate of the organism at 50 nm concentration. | 1991 | 16348474 |
| [archaeal diversity associated with sponge pachychalina sp. analyzed by culture-independent approach]. | with culture-independent approach, microbial total dna was directly extracted from pachychalina sp. using the total microbial dna as template, archaeal 16s rdnas were amplified by pcr with universal primers. amplified products were cloned into t-vector and secondarily amplified by pcr. then the secondarily amplified products were purified to be further characterized by termed ardra (amplified rdna restriction analysis). according to the enzyme restriction mapping, the apparent difference among t ... | 2005 | 15847177 |