| transcription in vitro and in vivo of the 7s rna gene associated with the ribosomal rna operon in the hyperthermophilic archaeon methanothermus fervidus. | the gene encoding the archaeal 7s rna in the hyperthermophile methanothermus fervidus is linked to a trna(ser) and rrna operon in the arrangement 5'-7s rna-14nt-trna(ser)-196nt-16s rrna and the promoter directing transcription of this 7s rna gene has now been identified. initiation of transcription of the 7s rna gene has been shown to occur both in vivo in m. fervidus and in vitro, using a methanococcus thermolithotrophicus derived cell-free transcription system, at the first g residue within th ... | 1992 | 1281131 |
| sequence of the 5-aminolevulinic acid dehydratase-encoding gene from the hyperthermophilic methanogen, methanothermus sociabilis. | we report on the sequence of the methanothermus sociabilis aladh gene, which encodes the 5'-aminolevulinic acid dehydratase. the identity of the enzyme was determined by sequence comparison and by expression in escherichia coli. | 1992 | 1398086 |
| hmf, a histone-related protein from the hyperthermophilic archaeon methanothermus fervidus, binds preferentially to dna containing phased tracts of adenines. | hmf, a histone-related protein from methanothermus fervidus, was found to bind preferentially to a dna that is intrinsically bent as a result of the presence of phased oligo(da) tracts. the intergenic regions in m. fervidus dna are a+t rich and frequently contain oligo(da) tracts, some of which may have the size and phasing required to create a net bending in one direction. the binding of hmf to bent dna could play a direct role in gene expression and stabilization of the genome of this organism ... | 1992 | 1447158 |
| hmt, a histone-related protein from methanobacterium thermoautotrophicum delta h. | hmt, a histone-related protein, has been isolated and characterized from methanobacterium thermoautotrophicum delta h. hmt preparations contain two polypeptides designated hmt1 and hmt2, encoded by the hmta and hmtb genes, respectively, that have been cloned, sequenced, and expressed in escherichia coli. hmt1 and hmt2 are predicted to contain 68 and 67 amino acid residues, respectively, and have calculated molecular masses of 7,275 and 7,141 da, respectively. aligning the amino acid sequences of ... | 1992 | 1459937 |
| identification and isolation of the polyferredoxin from methanobacterium thermoautotrophicum strain delta h. | sequencing the genes encoding the methyl viologen-reducing hydrogenase, cloned from methanobacterium thermoautotrophicum strain delta h and methanothermus fervidus, revealed the presence of tightly linked genes, designated mvhb, which were predicted to encode proteins containing six tandemly arranged bacterial ferrodoxin-like domains. a lacz-mvhb gene fusion has been constructed and expressed in escherichia coli. rabbit antibodies raised against the fusion polypeptide purified from e. coli have ... | 1992 | 1495982 |
| relatedness of the flagellins from methanogens. | purified flagellar filaments isolated from six methanogens were composed of multiple flagellins. two flagellins were present in methanococcus deltae (mr = 34,000 and 32,000), methanoculleus marisnigri (mr = 31,000 and 25,500) and methanococcus jannaschii (mr = 31,000 and 27,500), three in methanothermus fervidus (mr = 34,000, 25,000 and 24,000) and four or more in both methanococcus vannielii and methanococcus maripaludis (mr ranging from 27,500 to 32,000). the flagellins of m. fervidus and m. d ... | 1992 | 1503530 |
| correlation between glycosylation of flagellin proteins and sensitivity of flagellar filaments to triton x-100 in methanogens. | the flagellins of methanospirillum hungatei strains jf1 and gp1, methanococcus deltae, and methanothermus fervidus are glycosylated. isolated flagellar filaments from these organisms are dissociated by low concentrations (0.5% (v/v)) of triton x-100. flagellar filaments from other methanogens (methanococcus voltae, methanococcus vannielii and methanoculleus marisnigri) composed of non-glycosylated flagellins are resistant to triton x-100 treatment. consequently, the isolation techniques (employi ... | 1992 | 1537540 |
| transcription in vivo and in vitro of the histone-encoding gene hmfb from the hyperthermophilic archaeon methanothermus fervidus. | immediately upstream of the hmfb gene, in a dna fragment cloned from methanothermus fervidus, are two identical tandemly repeated copies of a 73-bp sequence that contain the sequence 5'tttatata, which conforms precisely to the consensus tata box element proposed for methanogen promoters. by using this duplicated region as the template dna and a cell-free transcription system derived from methanococcus thermolithotrophicus, transcription in vitro was found to initiate at two identical sites 73 bp ... | 1992 | 1592806 |
| dna binding by the archaeal histone hmf results in positive supercoiling. | hmf, a histone from the hyperthermophilic archaeon methanothermus fervidus binds double-stranded dna molecules in vitro, forming compact structures that visibly resemble eukaryal nucleosomes. we show here that hmf binding increases the helical periodicity of dna molecules to approximately 11 base pairs (bp) per turn and that dna molecules in these nucleosome-like structures are constrained in positive toroidal supercoils. based on the mass of hmf needed to cause a change in linking number (delta ... | 1991 | 1660135 |
| analysis and nucleotide sequence of the genes encoding the surface-layer glycoproteins of the hyperthermophilic methanogens methanothermus fervidus and methanothermus sociabilis. | the genes (slga) encoding the surface-layer glycoproteins of the hyperthermophilic methanogens methano-thermus pervidus and methanothermus sociabilis were cloned and sequenced. the nucleotide sequences of these genes differ at only nine positions, resulting in three amino acid differences. in both organisms, the transcription start site was localized by primer extension analyses. the dna sequence at this site conforms to the promotor box b motif for promotors of archaea. 24 nucleotides upstream ... | 1991 | 1712296 |
| properties and primary structure of the l-malate dehydrogenase from the extremely thermophilic archaebacterium methanothermus fervidus. | l-malate dehydrogenase from the extremely thermophilic mathanogen methanothermus fervidus was isolated and its phenotypic properties were characterized. the primary structure of the protein was deducted from the coding gene. the enzyme is a homomeric dimer with a molecular mass of 70 kda, possesses low specificity for nad+ or nadp+ and catalyzes preferentially the reduction of oxalacetate. the temperature dependence of the activity as depicted in the arrhenius and van't hoff plots shows disconti ... | 1990 | 2110059 |
| conservation of hydrogenase and polyferredoxin structures in the hyperthermophilic archaebacterium methanothermus fervidus. | a 3.3-kilobase-pair region of the methanothermus fervidus genome encoding part of the small subunit and all of the large subunit of the methyl viologen-reducing hydrogenase and a polyferredoxin was cloned and sequenced. the sequence of this hyperthermophilic hydrogenase conforms to the consensus sequence established for procaryotic [nife] hydrogenases. although the m. fervidus polyferredoxin is the same size as the methanobacterium thermoautotrophicum ferredoxin, containing six tandemly arranged ... | 1990 | 2115877 |
| genes encoding the 7s rna and trna(ser) are linked to one of the two rrna operons in the genome of the extremely thermophilic archaebacterium methanothermus fervidus. | analysis of gene structure in the extremely thermophilic archaebacterium, methanothermus fervidus, has revealed the presence of a cluster of stable rna-encoding genes arranged 5'-7s rna-trna(ser)-16s rrna-trna(ala)-23s rrna-5s rrna. the genome of m. fervidus contains two rrna operons but only one operon has the closely linked 7s rna-encoding gene. the sequences upstream from the two rrna operons are identical for 206 bp but diverge at the 3' base of the trna(ser) gene. the secondary structures p ... | 1990 | 2116370 |
| engineering thermostability in archaebacterial glyceraldehyde-3-phosphate dehydrogenase. hints for the important role of interdomain contacts in stabilizing protein conformation. | construction of hybrid enzymes between the glyceraldehyde-3-phosphate dehydrogenases from the mesophilic methanobacterium bryantii and the thermophilic methanothermus fervidus by recombinant dna techniques revealed that a short c-terminal fragment of the mt. fervidus enzyme contributes largely to its thermostability. this c-terminal region appears to be homologous to the alpha 3-helix of eubacterial and eukaryotic glyceraldehyde-3-phosphate dehydrogenases which is involved in the contacts betwee ... | 1990 | 2124542 |
| glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaebacterium pyrococcus woesei: characterization of the enzyme, cloning and sequencing of the gene, and expression in escherichia coli. | the glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaebacterium pyrococcus woesei (optimal growth temperature, 100 to 103 degrees c) was purified to homogeneity. this enzyme was strictly phosphate dependent, utilized either nad+ or nadp+, and was insensitive to pentalenolactone like the enzyme from the methanogenic archaebacterium methanothermus fervidus. the enzyme exhibited a considerable thermostability, with a 44-min half-life at 100 degrees c. the amino acid sequence ... | 1990 | 2165475 |
| biosynthesis of cyclic 2,3-diphosphoglycerate. isolation and characterization of 2-phosphoglycerate kinase and cyclic 2,3-diphosphoglycerate synthetase from methanothermus fervidus. | starting from 2-phosphoglycerate the biosynthesis of cdpg comprises two steps: (i) the phosphorylation of 2-phosphoglycerate to 2,3-diphosphoglycerate and (ii) the intramolecular cyclization to cyclic 2,3-diphosphoglycerate. the involved enzymes, 2-phosphoglycerate kinase and cyclic 2,3-diphosphoglycerate synthetase, were purified form methanothermus fervidus. their molecular and catalytic properties were characterized. | 1990 | 2226838 |
| hmf, a dna-binding protein isolated from the hyperthermophilic archaeon methanothermus fervidus, is most closely related to histones. | methanothermus fervidus grows optimally at 83 degrees c. a protein designated hmf (histone m. fervidus) has been isolated from this archaeal hyperthermophile that binds to double-stranded dna molecules and increases their resistance to thermal denaturation. hmf binding to linear double-stranded dna molecules of greater than 2 kilobase pairs also increases their electrophoretic mobilities through agarose gels. visualization of this compaction process by electron microscopy has demonstrated the fo ... | 1990 | 2377617 |
| cloning and sequencing the gene encoding 3-phosphoglycerate kinase from mesophilic methanobacterium bryantii and thermophilic methanothermus fervidus. | the nucleotide sequences of the gene (pgk) encoding 3-phosphoglycerate kinase (pgk) from the mesophilic archaebacterium, methanobacterium bryantii, and from the closely related thermophile, methanothermus fervidus, were determined. the deduced amino acid (aa) sequences show 61% identity with each other and 32-36% identity with the enzyme homologues from eubacteria and eukaryotes. as found for glyceraldehyde-3-phosphate dehydrogenase (gapdh) and l-malate dehydrogenase, the relatedness between the ... | 1990 | 2401408 |
| genes encoding 5s rrna and trnas in the extremely thermophilic archaebacterium methanothermus fervidus. | methanothermus fervidus was shown to have two 5s rrna-encoding genes linked in rrna operons to 16s and 23s rrna-encoding genes. sequencing of a cloned 5s rrna gene confirmed that m. fervidus is a member of the methanobacteriales, although its 5s rrna is also similar in both primary sequence and predicted secondary structure to the 5s rrna of the non-methanogenic, but also extremely thermophilic archaebacterium, thermococcus celer. two clusters of trna genes have also been cloned and sequenced fo ... | 1989 | 2473943 |
| nucleotide sequence of the glyceraldehyde-3-phosphate dehydrogenase gene from the mesophilic methanogenic archaebacteria methanobacterium bryantii and methanobacterium formicicum. comparison with the respective gene structure of the closely related extreme thermophile methanothermus fervidus. | the genes for glyceraldehyde-3-phosphate dehydrogenase (gap genes) from the mesophilic methanogenic archaebacteria methanobacterium formicicum and methanobacterium bryantii were cloned and sequenced. the deduced amino acid sequences show 95% identity to each other and about 70% identity to the glyceraldehyde-3-phosphate dehydrogenase from the thermophilic methanogenic archaebacterium methanothermus fervidus. although the sequence similarity between the archaebacterial glyceraldehyde-3-phosphate ... | 1989 | 2492940 |
| a comparison of the methyl reductase genes and gene products. | the dna sequences encoding component c of methyl coenzyme m reductase (mcr genes) in methanothermus fervidus, methanobacterium thermoautotrophicum, methanococcus vannielii, and methanosarcina barkeri have been published. comparisons of transcription initiation and termination sites and of the amino acid sequences of the mcr gene products are presented. structural features conserved within the amino acid sequences are identified and a comparison of methyl reductase with other disulfide bond synth ... | 1989 | 2720489 |
| primary structure of glyceraldehyde-3-phosphate dehydrogenase deduced from the nucleotide sequence of the thermophilic archaebacterium methanothermus fervidus. | the gene for the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (gapdh) from the thermophilic methanogenic archaebacterium methanothermus fervidus (growth optimum at 84 degrees c) was cloned in escherichia coli and the nucleotide sequence was determined. a striking preference for adenine and thymidine bases was found in the gene, which is in agreement with the low g + c content of the m. fervidus dna. the deduced amino acid sequence indicates an mr of 37,500 for the protein subunit. ... | 1988 | 2841192 |
| expression of the glyceraldehyde-3-phosphate dehydrogenase gene from the extremely thermophilic archaebacterium methanothermus fervidus in e. coli. enzyme purification, crystallization, and preliminary crystal data. | the gene of glyceraldehyde-3-phosphate dehydrogenase (gapdh) from the extremely thermophilic archaebacterium methanothermus fervidus (growth optimum 82 degrees c) was cloned in vector pjf118eh and expressed in e. coli cells. as shown by molecular mass determination, protein sequencing, heat stability, and substrate saturation kinetics, the enzyme synthesized in e. coli is identical to the original enzyme from m. fervidus. the high thermostability of the e. coli-produced m. fervidus gapdh allows ... | 1988 | 3049151 |
| dna-dependent rna polymerases of the three orders of methanogens. | the dna-dependent rna polymerases of members of the three orders of methanogens were purified and their enzymatic properties described. the enzymes consist of 7-8 polypeptides. although these differed in molecular mass, the four heaviest components could be allied to components of the enzyme of methanobacterium thermoautotrophicum, w by cross-reaction with antibodies directed against the denatured polypeptides of this enzyme. the antisera against native rna polymerases isolated from representati ... | 1986 | 3091049 |
| structure and comparative analysis of the genes encoding component c of methyl coenzyme m reductase in the extremely thermophilic archaebacterium methanothermus fervidus. | a 6-kilobase-pair (kbp) region of the genome of the extremely thermophilic arachaebacterium methanothermus fervidus which encodes the alpha, beta, and gamma subunit polypeptides of component c of methyl coenzyme m reductase was cloned and sequenced. genes encoding the beta (mcrb) and gamma (mcrg) subunits were separated by two open reading frames (designated mcrc and mcrd) which encode unknown gene products. the m. fervidus genes were preceded by ribosome-binding sites, separated by short a + t- ... | 1988 | 3170483 |
| purification and characterization of d-glyceraldehyde-3-phosphate dehydrogenase from the thermophilic archaebacterium methanothermus fervidus. | the d-glyceraldehyde-3-phosphate dehydrogenase from the extremely thermophilic archaebacterium methanothermus fervidus was purified and crystallized. the enzyme is a homomeric tetramer (molecular mass of subunits 45 kda). partial sequence analysis shows homology to the enzymes from eubacteria and from the cytoplasm of eukaryotes. unlike these enzymes, the d-glyceraldehyde-3-phosphate dehydrogenase from methanothermus fervidus reacts with both nad+ and nadp+ and is not inhibited by pentalenolacto ... | 1987 | 3569291 |
| taxonomic relations between archaebacteria including 6 novel genera examined by cross hybridization of dnas and 16s rrnas. | dnas from 16 species of archaebacteria including 6 novel isolates were hybridized with 16s rrnas from 7 species representing different orders or groups of the urkingdom of archaebacteria. the yields, normalized for the number of genes per microgram of dna, and the temperature stabilities of all hybrids were determined and related to each other. a taxonomic tree constructed from such fractional stability data reveals the same major divisions as that derived from comparative cataloging of 16s rrna ... | 1982 | 6178834 |
| cloning, sequencing and transcript analysis of the gene encoding formylmethanofuran: tetrahydromethanopterin formyltransferase from the hyperthermophilic methanothermus fervidus. | the formylmethanofuran:tetrahydromethanopterin formyltransferase (ftr) from methanothermus fervidus was partially purified and its n-terminal amino acid sequence determined. using as probe a mixture of oligonucleotides derived from the ftr n-terminus, the corresponding gene (ftr) was cloned and sequenced. the ftr gene codes for 297 amino acids, corresponding to a molecular mass of 31,836 daltons, in contrast to the 41,000 daltons estimated for the protein by sodium dodecylsulphate-polyacrylamide ... | 1994 | 7506350 |
| dimeric 3-phosphoglycerate kinases from hyperthermophilic archaea. cloning, sequencing and expression of the 3-phosphoglycerate kinase gene of pyrococcus woesei in escherichia coli and characterization of the protein. structural and functional comparison with the 3-phosphoglycerate kinase of methanothermus fervidus. | the gene coding for the 3-phosphoglycerate kinase (ec 2.7.2.3) of pyrococcus woesei was cloned and sequenced. the gene sequence comprises 1230 bp coding for a polypeptide with the theoretical m(r) of 46,195. the deduced protein sequence exhibits a high similarity (46.1% and 46.6% identity) to the other known archaeal 3-phosphoglycerate kinases of methanobacterium bryantii and methanothermus fervidus [fabry, s., heppner, p., dietmaier, w. & hensel, r. (1990) gene 91, 19-25]. by comparing the 3-ph ... | 1995 | 7588750 |
| structure and stability of histone hmf from the hyperthermophilic archaeon methanothermus fervidus. | the secondary and quaternary structures and stabilities of recombinant (r) forms of the hmfa and hmfb histones from methanothermus fervidus have been investigated by cd spectroscopy and formaldehyde-mediated protein-protein cross-linking. both proteins were shown to be dimers in solutions containing 5-1300 mm kcl, at ph 6-10 and 25-83 degrees c, and specifically in 1 m kcl, at ph 7.5 and 83 degrees c, conditions which approximate those in vivo in m. fervidus cells. heat treatment of a mixture of ... | 1995 | 7599135 |
| formylmethanofuran:tetrahydromethanopterin formyltransferase (ftr) from the hyperthermophilic methanopyrus kandleri. cloning, sequencing and functional expression of the ftr gene and one-step purification of the enzyme overproduced in escherichia coli. | methanopyrus kandleri is a methanogenic archaeon that grows on h2 and co2 at a temperature optimum of 98 degrees c. the gene ftr encoding the formylmethanofuran:tetrahydromethanopterin formyltransferase, an enzyme involved in co2 reduction to methane, has been cloned, sequenced, and overexpressed in escherichia coli. the overproduced enzyme could be purified in yields above 90% by simply heating the cell extract to 90 degrees c in 1.5 m k2hpo4 ph 8.0 for 30 min. from 1 g wet cells (70 mg protein ... | 1995 | 7601152 |
| eukaryotic methionyl aminopeptidases: two classes of cobalt-dependent enzymes. | using partial amino acid sequence data derived from porcine methionyl aminopeptidase (metap; methionine aminopeptidase, peptidase m; ec 3.4.11.18), a full-length clone of the homologous human enzyme has been obtained. the cdna sequence contains 2569 nt with a single open reading frame corresponding to a protein of 478 amino acids. the c-terminal portion representing the catalytic domain shows limited identity with metap sequences from various prokaryotes and yeast, while the n terminus is rich i ... | 1995 | 7644482 |
| growth-phase-dependent synthesis of histones in the archaeon methanothermus fervidus. | histone preparations from methanothermus fervidus (hmf) contain two small polypeptides, hmfa and hmfb, which in solution are dimers and compact dna to form nucleosome-like structures. these archaeal nucleosome-like structures constrain positive dna supercoils, in contrast to the negatively supercoiled dna in eukaryal nucleosomes. hmfa has been found to make up as much as 80% of hmf preparations synthesized by m. fervidus cells during the exponential growth phase of batch cultures but to decrease ... | 1994 | 7809089 |
| an archaeal s-layer gene homolog with repetitive subunits. | an s-layer protein gene homolog of the slga gene of two methanothermus species was found in the genome of another methanogenic archaeon of a different family, methanosarcina mazei s-6. the new gene (slgb) encodes a molecule (slpb) with the characteristics of s-layer proteins. the n-terminal half of slpb is 44% identical to that encoded by slga, but the other half shows distinctive features: four 56 amino acid long tandem repeats, and trp-xaa-trp clusters located six amino acids from the n-termin ... | 1994 | 7948028 |
| on the evolution of arginases and related enzymes. | sequence analysis of the arginase/agmatine ureohydrolase family, important enzymes in arginine/agmatine metabolism and the urea cycle, reveals the similarity of arginases to formiminoglutamate hydrolase (hutg) in klebsiella aerogenes and to a previously unidentified open reading frame adjacent to the hmf locus of the archaebacterium methanothermus fervidus. the gene structure and distribution of these homologous proteins across primary kingdoms suggest that this family is another example of a pr ... | 1994 | 8064866 |
| cloning, sequencing and expression of the gene encoding 2-phosphoglycerate kinase from methanothermus fervidus. | the gene encoding 2-phosphoglycerate kinase (2pgk), which catalyses the first step in the biosynthesis of cyclic 2,3-diphosphoglycerate in methanogens, was cloned and sequenced from the hyperthermophilic methanothermus fervidus. the 2pgk gene codes for 304 amino acids, corresponding to a relative molecular mass of 35040. the 2pgk mrna was estimated to be 1600 nucleotides in size. putative transcription signals and the ribosome-binding site of 2pgk are discussed. production of 2pgk from m. fervid ... | 1994 | 8159166 |
| characterization and phylogeny of mcrii, a gene cluster encoding an isoenzyme of methyl coenzyme m reductase from hyperthermophilic methanothermus fervidus. | a 5.7 kb region of chromosomal dna from methanothermus fervidus, harbouring a second mcr gene cluster, was cloned and sequenced. this gene cluster, termed mcrii, encodes an isoenzyme of methyl coenzyme m reductase (mcr). in contrast to the known mcr gene clusters from other methanogens, mcrii lacks mcrc, a gene of unknown function. but the remaining mcrii genes b, d, g and a are arranged in the same order as in previously sequenced mcr gene clusters. the mcrii genes from m. fervidus are located ... | 1994 | 8177216 |
| primary structure of the heterosaccharide of the surface glycoprotein of methanothermus fervidus. | the outer surface of the cells of the hyperthermophile methanothermus fervidus is covered by crystalline glycoprotein subunits (s-layer). from the purified s-layer glycoprotein, a heterosaccharide was isolated. the heterosaccharide consists of d-3-o-methylmannose, d-mannose, and d-n-acetylgalactosamine in a molar ratio of 2:3:1 corresponding to a relative molecular mass of 1061.83 da. 3-o-methylmannose could be partly replaced by 3-o-methylglucose. the primary structure of the glycan was reveale ... | 1993 | 8262914 |
| the alcaligenes eutrophus ldh structural gene encodes a novel type of lactate dehydrogenase. | the lactate dehydrogenase gene, ldh, of alcaligenes eutrophus h16 was identified on a 14-kbp ecori restriction fragment of a genomic library in the cosmid phc79 by hybridization with a 50-mer synthetic oligonucleotide which was derived from the n-terminal amino acid sequence of the purified enzyme. recombinant strains of escherichia coli jm83, which harboured a 2.0-kbp psti subfragment in puc9-1, expressed ldh at a high level, if ldh was downstream from and colinear to the e. coli lac promoter. ... | 1993 | 8405966 |
| the phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase genes from the thermophilic archaeon sulfolobus solfataricus overlap by 8-bp. isolation, sequencing of the genes and expression in escherichia coli. | the overlapping genes encoding phosphoglycerate kinase (pgk) and glyceraldehyde-3-phosphate dehydrogenase (grap-dh) from the hyperthermophilic archaeon sulfolobus solfataricus have been cloned and sequenced. pcr primers based on highly conserved regions of different pgk sequences were used to isolate an internal region of the pgk gene. this was then used to screen a genomic library to isolate the full length pgk gene. a 2.5-kb bglii fragment of s. solfataricus dna contained both the pgk gene and ... | 1995 | 8521845 |
| nmr structure of hmfb from the hyperthermophile, methanothermus fervidus, confirms that this archaeal protein is a histone. | the three-dimensional structure of the recombinant histone rhmfb from methanothermus fervidus, an archaeon that grows optimally at 83 degrees c, has been determined by nuclear magnetic resonance methods. this is only the third structure of a protein from a hyperthermophilic organism (optimal growth at temperatures above 80 degrees c). signal assignments were made using a combination of homonuclear-correlated, 15n-double resonance and 15n, 13c triple resonance nmr experiments. long range dipolar ... | 1996 | 8568866 |
| primary structure and properties of the formyltransferase from the mesophilic methanosarcina barkeri: comparison with the enzymes from thermophilic and hyperthermophilic methanogens. | the ftr gene encoding formylmethanofuran: tetrahydromethanopterin formyltransferase (ftr) from methanosarcina barkeri was cloned, sequenced, and functionally expressed in escherichia coli. the overproduced enzyme was purified eightfold to apparent homogeneity, and its catalytic properties were determined. the primary structure and the hydropathic character of the formyltransferase from methanosarcina barkeri were compared with those of the enzymes from methanobacterium thermoautotrophicum, metha ... | 1996 | 8593103 |
| comparative analysis of ribonuclease p rna structure in archaea. | although the structure of the catalytic rna component of ribonuclease p has been well characterized in bacteria, it has been little studied in other organisms, such as the archaea. we have determined the sequences encoding rnase p rna in eight euryarchaeal species: halococcus morrhuae, natronobacterium gregoryi, halobacterium cutirubrum, halobacteriurn trapanicum, methanobacterium thermoautotrophicum strains deltah and marburg, methanothermus fervidus and thermococcus celer strain al-1. on the b ... | 1996 | 8614627 |
| histones and chromatin structure in hyperthermophilic archaea. | hmf is a histone from the hyperthermophile methanothermus fervidus. it is the archetype and most studied member of a family of archaeal histones that have primary sequences and three-dimensional structures in common with the eukaryal nucleosome core histones and that bind and compact dna molecules into nucleosome-like structures (nls). hmf preparations are mixtures of two similar, small (approximately 7.5 kda) polypeptides designated hmfa and hmfb that in vivo form both homodimers and heterodime ... | 1996 | 8639328 |
| purification and characterization of phosphoenolpyruvate carboxylase from the hyperthermophilic archaeon methanothermus sociabilis. | phosphoenolpyruvate carboxylase (pepc) was purified for the first time from hyperthermophilic archaeon methanothermus sociabilis, growing autotrophically with an optimum at 88 degrees c. the optimum temperature for enzyme activity was similar to that for growth and was 85 degrees c. the native enzyme was a homotetramer of 240 kda molecular mass and the subunit displayed an apparent molecular mass of 60 kda. the archaeal pepc was insensitive to various metabolites which are known as allosteric ef ... | 1996 | 8772193 |
| crystallization and preliminary x-ray characterization of the methanothermus fervidus histones hmfa and hmfb. | hmfa and hmfb are histone proteins from the thermophilic archaeon methanothermus fervidus. they wrap dna into nucleosome-like structures and appear to represent the basic core histone fold. hmfa was crystallized in space groups p4(2)2(1)2 and p2(1)2(1)2(1). hmfb crystallized in space group p2(1)2(1)2, while a selenomethionine-substituted variant, semet-hmfb, yielded crystals in c222(1). in all crystal forms hmfa, hmfb, or semet-hmfb may be present as homodimers. | 1996 | 8820495 |
| purification and characterization of a histone-like protein from the archaeal isolate an1, a member of the thermococcales. | we have purified and characterized the histone-like protein, termed han1, and an han1-associated dna-binding protein (hdbp) from nucleoids of the hyperthermophilic thermococcus-like an1. han1 is shown to be composed of two subunits, to be thermally stable and to compact dna in a reversible manner. the n-terminal sequence of han1 shares a high degree of homology with hmf, the histone-like protein from methanothermus fervidus. consistent with this, the toroidal wrapping of dna by han1 resembles th ... | 1996 | 8861206 |
| tetrameric triosephosphate isomerase from hyperthermophilic archaea. | triosephosphate isomerase (tim) of the hyperthermophilic archaea pyrococcus woesei and methanothermus fervidus have been purified to homogeneity. the enzymes from the two hyperthermophiles represent homo-tetramers of 100 kda, contrary to all known bacterial and eukaryotic tims, which are dimers of 48-60 kda. molecular size determination of the tim from the mesophilic methanogen methanobacterium bryantii yielded the usual molecular mass of only 57 kda, indicating that the tetrameric aggregation s ... | 1996 | 8925906 |
| heat-shock response in methanosarcina mazei s-6. | the dnak locus of methanosarcina mazei s-6, a mesophilic organism of the phylogenetic domain archaea, contains the heat-shock genes 5'-grpe-dnak-dnaj-3'. parameters known to affect the response of these genes in organisms of the other two domains, bacteria and eucarya, were tested to determine their effects on the archaeal homologs. the mrna from the three genes increased after heat shock more in lamina than in single cells (these s-6 morphologic stages can be grown in the same substrate). singl ... | 1997 | 9216886 |
| archaeal nucleosomes. | archaea contain histones that have primary sequences in common with eukaryal nucleosome core histones and a three-dimensional structure that is essentially only the histone fold. here we report the results of experiments that document that archaeal histones compact dna in vivo into structures similar to the structure formed by the histone (h3+h4)2 tetramer at the center of the eukaryal nucleosome. after formaldehyde cross-linking in vivo, these archaeal nucleosomes have been isolated from methan ... | 1997 | 9356501 |
| sequence and structural comparison of thermophilic phosphoglycerate kinases with a mesophilic equivalent. | the monomeric glycolytic enzyme phosphoglycerate kinase (pgk) has been used as a model system to study protein thermostability. the primary sequence of this enzyme has been elucidated from 47 species to date. although only 42 amino acids are totally conserved, most of which line the active site cleft, the protein is structurally conserved. this is achieved by making conservative changes to maintain the same secondary and tertiary folds. the crystal structures of 5 pgk enzymes have been solved by ... | 1997 | 9406428 |
| a novel ether core lipid with h-shaped c80-isoprenoid hydrocarbon chain from the hyperthermophilic methanogen methanothermus fervidus. | a new ether lipid core (designated as fu) was found in methanothermus fervidus total lipid. comparison with caldarchaeol showed lower mobility of fu on tlc and smaller molecular weight (m/z 1298) by 2 mass units on fab-ms. treatment of fu with hi followed by displacement with silver acetate afforded long chain alcohol acetate (roac), which was further saponified with mild alkali to its free alcohol (roh). roh is the long chain alcohol prepared from fu. the molecular weights of roac and roh were ... | 1998 | 9487155 |
| roles of conserved residues in the arginase family. | arginases and related enzymes metabolize arginine or similar nitrogen-containing compounds to urea or formamide. in the present report a sequence alignment of 31 members of this family was generated. the alignment, together with the crystal structure of rat liver arginase, allowed the assignment of possible functional or structural roles to 32 conserved residues and conservative substitutions. two of these residues were previously identified as functionally essential by analysis of inherited def ... | 1998 | 9507056 |
| comparative study of the catalytic domain of phosphorylating glyceraldehyde-3-phosphate dehydrogenases from bacteria and archaea via essential cysteine probes and site-directed mutagenesis. | phosphorylating glyceraldehyde-3-phosphate dehydrogenase (grap-dh) catalyzes the oxidative phosphorylation of d-glyceraldehyde-3-phosphate to form 1.3-diphosphoglycerate. the currently accepted mechanism involves an oxidoreduction step followed by a phosphorylation. two essential aminoacids, cys149 and his176 are involved in the chemical mechanism of bacterial and eukaryotic grap-dhs. roles have been assigned to the his176 as (a) a chemical activator for enhancing the reactivity of cys149, (b) a ... | 1998 | 9546660 |
| improved n-terminal processing of recombinant proteins synthesized in escherichia coli. | preparations of rhmfa (recombinant histone a from methanothermus fervidus) synthesized in e. coli by the heterologous expression of the hmfa gene were found to contain a mixture of rhmfa molecules, approximately 40% that retained the n-terminal formyl-methionyl residue (f-met-rhmfa), approximately 50% that lacked the formyl moiety but retained the methionyl residue (met-rhmfa), and only approximately 10% that had lost both components of the protein synthesis initiating amino acid residue and the ... | 1995 | 9634792 |
| two malate dehydrogenases in methanobacterium thermoautotrophicum. | methanobacterium thermoautotrophicum (strain marburg) was found to contain two malate dehydrogenases, which were partially purified and characterized. one was specific for nad+ and catalyzed the dehydrogenation of malate at approximately one-third of the rate of oxalacetate reduction, and the other could equally well use nad+ and nadp+ as coenzyme and catalyzed essentially only the reduction of oxalacetate. via the n-terminal amino acid sequences, the encoding genes were identified in the genome ... | 1998 | 9639601 |
| archaeal histone stability, dna binding, and transcription inhibition above 90 degrees c. | the dna binding and compacting activities of the recombinant (r) archaeal histones rhmfa and rhmfb from methanothermus fervidus, and rhpya1 from pyrococcus species gb-3a, synthesized in escherichia coli, have been shown to be completely resistant to incubation for 4h at 95 degrees c in the presence of 1m kcl. continued incubation of rhmfa and rhmfb at 95 degrees c resulted in a gradual loss of these activities, and rhmfa and rhmfb lost activity more rapidly at 95 degrees c when the salt environm ... | 1998 | 9672681 |
| dna binding and nuclease protection by the hmf histones from the hyperthermophilic archaeon methanothermus fervidus. | the dna-binding and nuclease-protection properties of the hmf histones from the hyperthermophilic archaeon methanothermus fervidus have been shown to be consistent with the formation of nucleosome-like structures (nls). these proteins bind to dna molecules as short as 20 bp and form complexes that protect dna fragments from micrococcal nuclease (mnase) digestion that are 30 bp, approximately 60 bp and multiples of approximately 60 bp in length. the sequences of 49 of the approximately 60-bp dna ... | 1997 | 9680306 |
| thermodynamic stability of archaeal histones. | the temperature, salt, and ph dependencies of unfolding of four recombinant (r) archaeal histones (rhfob from the mesophile methanobacterium formicicum, and rhmfa, rhmfb, and rhpya1 from the hyperthermophiles methanothermus fervidus and pyrococcus strain gb-3a) have been determined by circular dichroism spectroscopy (cd) and differential scanning calorimetry (dsc). the thermal unfolding of these proteins is > 90% reversible, with concentration-dependent apparent tm values and asymmetric unfoldin ... | 1998 | 9692945 |
| nmr structure and comparison of the archaeal histone hfob from the mesophile methanobacterium formicicum with hmfb from the hyperthermophile methanothermus fervidus. | the solution-state structure of the recombinant archaeal histone rhfob, from the mesophile methanobacterium formicicum, has been determined by two- and three-dimensional (3d) proton homonuclear correlated nuclear magnetic resonance (nmr) methods. on the basis of 951 nuclear overhauser effect (noe)-derived distance restraints, rhfob monomers form the histone fold and assemble into symmetric (rhfob)2 dimers that have a structure consistent with assembly into archaeal nucleosomes. rhfob exhibits ap ... | 1998 | 9692946 |
| cloning, sequencing, and expression of the gene encoding cyclic 2, 3-diphosphoglycerate synthetase, the key enzyme of cyclic 2, 3-diphosphoglycerate metabolism in methanothermus fervidus. | cyclic 2,3-diphosphoglycerate synthetase (cdpgs) catalyzes the synthesis of cyclic 2,3-diphosphoglycerate (cdpg) by formation of an intramolecular phosphoanhydride bond in 2,3-diphosphoglycerate. cdpg is known to be accumulated to high intracellular concentrations (>300 mm) as a putative thermoadapter in some hyperthermophilic methanogens. for the first time, we have purified active cdpgs from a methanogen, the hyperthermophilic archaeon methanothermus fervidus, sequenced the coding gene, and ex ... | 1998 | 9811660 |
| archaeal nucleosome positioning sequence from methanothermus fervidus. | dna in methanothermus fervidus, a hyperthermophilic archaeon, is constrained into archaeal nucleosomes in vivo by the archaeal histones hmfa and hmfb. here, we document the translational and rotational positioning of archaeal nucleosome assembly in vitro by a sequence from the 7s rna encoding region of the m. fervidus genome. the minor groove of the dna at the center of the dna sequence, protected from micrococcal nuclease digestion by incorporation into a positioned archaeal nucleosome, faces a ... | 1999 | 10369753 |
| crystallization and preliminary x-ray diffraction studies of d-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon methanothermus fervidus. | the homotetrameric holo-d-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon methanothermus fervidus has been crystallized in the presence of nadp+ using the hanging-drop vapour-diffusion method. crystals grew from a solution containing 2-methyl-2,4-pentanediol and magnesium acetate. a native data set has been collected to 2.1 a using synchrotron radiation and cryocooling. diffraction data have been processed in the orthorhombic system (space group p21212) with unit-cel ... | 1999 | 10393306 |
| functional characterization of the phosphorylating d-glyceraldehyde 3-phosphate dehydrogenase from the archaeon methanothermus fervidus by comparative molecular modelling and site-directed mutagenesis. | phosphorylating archaeal d-glyceraldehyde 3-phosphate dehydrogenases (grap-dhs) share only 15-20% identity with their glycolytic bacterial and eukaryotic counterparts. unlike the latter which are nad-specific, archaeal grap-dhs exhibit a dual-cofactor specificity with a marked preference for nadp. in the present study, we have constructed a three-dimensional model of the methanothermus fervidus grap-dh based upon the x-ray structures of the bacillus stearothermophilus and escherichia coli grap-d ... | 1999 | 10491162 |
| [use of the level of g+c n dna for studying the molecular phylogeny o f methanogenic archaebacteria]. | a new method for theoretical analysis of the molecular phylogeny of bacteria, successfully applied earlier to nitrifying bacteria, was used to study the molecular phylogeny of methanogenic archaebacteria. the group studied included methanococcus igneus, methanococcus vannielii, methanothermus fervidus, methanolobus tindarius, methanobacterium formicicum, methanosarcina barkeri, methanobacterium thermoformicicum, methanoplanus limicola, methanospirillum hungatei, and methanobacterium thermoautotr ... | 1999 | 10624685 |
| mutational analysis of differences in thermostability between histones from mesophilic and hyperthermophilic archaea. | amino acid residues responsible for the large difference in thermostability between hmfb and hfob, archaeal histones from the hyperthermophile methanothermus fervidus and the mesophile methanobacterium formicicum, respectively, have been identified by site-specific mutagenesis. the thermal denaturation of approximately 70 archaeal histone variants has been monitored by circular dichroism, and the data generated were fit to a two-state unfolding model (dimer-->two random coil monomers) to obtain ... | 2000 | 10633118 |
| mutational analysis of archaeal histone-dna interactions. | site-specific mutagenesis of the hmfb gene cloned from the archaeon methanothermus fervidus, followed by expression in escherichia coli, has been used to generate approximately 90 recombinant (r) variants of the archaeal histone hmfb. the abilities of these variants to form stable archaeal nucleosome-containing complexes with linear pbr322 dna, and with an 89 bp restriction fragment of this dna have been determined. variants that failed to form such complexes, based on negative gel-shift assays, ... | 2000 | 10704305 |
| the crystal structure of d-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon methanothermus fervidus in the presence of nadp(+) at 2.1 a resolution. | the crystal structure of the glyceraldehyde-3-phosphate dehydrogenase (gapdh) from the archaeon methanothermus fervidus has been solved in the holo form at 2.1 a resolution by molecular replacement. unlike bacterial and eukaryotic homologous enzymes which are strictly nad(+)-dependent, gapdh from this organism exhibits a dual-cofactor specificity, with a marked preference for nadp(+) over nad(+). the present structure is the first archaeal gapdh crystallized with nadp(+). gapdh from m. fervidus ... | 2000 | 10715215 |
| identification of an archaeal 2-hydroxy acid dehydrogenase catalyzing reactions involved in coenzyme biosynthesis in methanoarchaea. | two putative malate dehydrogenase genes, mj1425 and mj0490, from methanococcus jannaschii and one from methanothermus fervidus were cloned and overexpressed in escherichia coli, and their gene products were tested for the ability to catalyze pyridine nucleotide-dependent oxidation and reduction reactions of the following alpha-hydroxy-alpha-keto acid pairs: (s)-sulfolactic acid and sulfopyruvic acid; (s)-alpha-hydroxyglutaric acid and alpha-ketoglutaric acid; (s)-lactic acid and pyruvic acid; an ... | 2000 | 10850983 |
| archaeal histone selection of nucleosome positioning sequences and the procaryotic origin of histone-dependent genome evolution. | archaeal histones and the eucaryal (eucaryotic) nucleosome core histones have almost identical histone folds. here, we show that dna molecules selectively incorporated by rhmfb (recombinant archaeal histone b from methanothermus fervidus) into archaeal nucleosomes from a mixture of approximately 10(14) random sequence molecules contain sequence motifs shown previously to direct eucaryal nucleosome positioning. the dinucleotides gc, aa (=tt) and ta are repeated at approximately 10 bp intervals, w ... | 2000 | 11021967 |
| crystal structures of recombinant histones hmfa and hmfb from the hyperthermophilic archaeon methanothermus fervidus. | the hyperthermophilic archaeon methanothermus fervidus contains two small basic proteins, hmfa (68 amino acid residues) and hmfb (69 residues) that share a common ancestry with the eukaryal nucleosome core histones h2a, h2b, h3, and h4. hmfa and hmfb have sequences that differ at 11 locations, they have different structural stabilities, and the complexes that they form with dna have different electrophoretic mobilities. here, crystal structures are documented for recombinant (r) hmfa at a resolu ... | 2000 | 11021968 |
| triose-phosphate isomerase from pyrococcus woesei and methanothermus fervidus. | | 2001 | 11265484 |
| polyamines of the hyperthermophilic archaebacteria belonging to the genera thermococcus and methanothermus and two new genera caldivirga and palaeococcus. | cellular polyamines of eight new thermophilic archaebacteria were investigated to determine the chemotaxonomic significance of polyamine distribution profiles. hyperthermoacidophilic caldivirga maquilingensis belonging to the family thermoproteaceae of the crenarchaeota have a unique polyamine profile comprising spermidine, norspermidine and norspermine as the major polyamines. within the order thermococcales of the euryarchaeota, the major polyamines of an extremely thermophilic terrestrial spe ... | 2001 | 11297011 |
| the first examples of (s)-2-hydroxyacid dehydrogenases catalyzing the transfer of the pro-4s hydrogen of nadh are found in the archaea. | reduction of 2-oxoacids to the corresponding (s)-2-hydroxyacids is an important transformation in biochemistry. to date all (s)-2-hydroxyacid dehydrogenases belonging to the l-lactate/l-malate dehydrogenase family have been found to transfer the pro-4r hydrogen of either nadh or nadph to c-2 of the 2-oxoacid substrates during their reduction. here, we report that recombinantly generated (s)-2-hydroxyacid dehydrogenases present in the methanoarchaea methanococcus jannaschii and methanothermus fer ... | 2001 | 11451450 |
| both dna and histone fold sequences contribute to archaeal nucleosome stability. | the roles and interdependence of dna sequence and archaeal histone fold structure in determining archaeal nucleosome stability and positioning have been determined and quantitated. the presence of four tandem copies of tttaaagccg in the polylinker region of plitmus28 resulted in a dna molecule with increased affinity (deltadeltag of approximately 700 cal mol(-1)) for the archaeal histone hmfb relative to the polylinker sequence, and the dominant, quantitative contribution of the helical repeats ... | 2002 | 11751933 |
| archaeal histone tetramerization determines dna affinity and the direction of dna supercoiling. | dna binding and the topology of dna have been determined in complexes formed by >20 archaeal histone variants and archaeal histone dimer fusions with residue replacements at sites responsible for histone fold dimer:dimer interactions. almost all of these variants have decreased affinity for dna. they have also lost the flexibility of the wild type archaeal histones to wrap dna into a negative or positive supercoil depending on the salt environment; they wrap dna into positive supercoils under al ... | 2002 | 12058041 |
| phospholipid etherlipid and phospholipid fatty acid fingerprints in selected euryarchaeotal monocultures for taxonomic profiling. | phospholipid etherlipid (plel) derived isoprenoids and phospholipid fatty acids (plfa) were determined in eight euryarchaeotal monocultures for taxonomic profiling. for the first time significant amounts of fatty acids in the plfa of euryarchaeota were determined. the plfa proportion varied between 11.3 and 35.5% of the total phospholipid side chains except in methanothermus fervidus where plfa accounted for 89.0% of the total phospholipid side chains. fractionation of fatty acids prior to gas c ... | 2002 | 12127500 |
| comparative analysis of thermoadaptation within the archaeal glyceraldehyde-3-phosphate dehydrogenases from mesophilic methanobacterium bryantii and thermophilic methanothermus fervidus. | to gain insight into the molecular determinants of thermoadaptation within the family of archaeal glyceraldehyde-3-phosphate dehydrogenases (gapdh), a homology-based 3-d model of the mesophilic gapdh from methanobacterium bryantii was built and compared with the crystal structure of the thermophilic gapdh from methanothermus fervidus. the homotetrameric model of the holoenzyme was initially assembled from identical subunits completed with nadp molecules. the structure was then refined by energy ... | 2002 | 12382287 |
| hyperthermophilic dehydrogenase enzymes. | archaeal dehydrogenases are often found to be of a specific class of dehydrogenase which has low sequence identity to the equivalent bacterial and eukaryotic counterparts. this paper focuses on two different types of hyperthermophilic dehydrogenase enzyme that have been cloned and over-expressed in escherichia coli. the crystallographic structures of the apo form of gapdh (glyceraldehyde-3-phosphate dehydrogenase) from sulfolobus solfataricus and the related holo form of gapdh from methanothermu ... | 2004 | 15046583 |
| stability and folding mechanism of mesophilic, thermophilic and hyperthermophilic archael histones: the importance of folding intermediates. | the equilibrium stabilities to guanidinium chloride (gdmcl)-induced denaturation and kinetic folding mechanisms have been characterized for three archael histones: hfob from the mesophile methanobacterium formicicum; hmfb from the thermophile methanothermus fervidus; and hpya1 from the hyperthermophile pyrococcus strain gb-3a. these histones are homodimers of 67 to 69 residues per monomer. the equilibrium unfolding transitions, as measured by far-uv circular dichroism (cd) are highly reversible, ... | 2004 | 15313621 |
| organic solutes in hyperthermophilic archaea. | we examined the accumulation of organic solutes under optimum growth conditions in 12 species of thermophilic and hyperthermophilic archaea belonging to the crenarchaeota and euryarchaeota. pyrobaculum aerophilum, thermoproteus tenax, thermoplasma acidophilum, and members of the order sulfolobales accumulated trehalose. pyrococcus furiosus accumulated di-myo-inositol-1,1(prm1)(3,3(prm1))-phosphate and (beta)-mannosylglycerate, methanothermus fervidus accumulated cyclic-2,3-bisphosphoglycerate an ... | 1997 | 16535556 |
| using archaeal histones for precise dna fragmentation. | the fragmentation of dna is a useful procedure for many molecular biology procedures. however, most methods used to fragment dna are poorly controllable, and cannot be used to create small fragments. we describe a method to generate random dna fragments of a predictable size to be cloned in expression vectors for the construction of display libraries. the dna is allowed to form complexes with archaeal histones from methanothermus fervidus (hmf) and the hmf/dna core complex is naturally protected ... | 2007 | 17545175 |
| thermodynamic stability and folding of proteins from hyperthermophilic organisms. | life grows almost everywhere on earth, including in extreme environments and under harsh conditions. organisms adapted to high temperatures are called thermophiles (growth temperature 45-75 degrees c) and hyperthermophiles (growth temperature >or= 80 degrees c). proteins from such organisms usually show extreme thermal stability, despite having folded structures very similar to their mesostable counterparts. here, we summarize the current data on thermodynamic and kinetic folding/unfolding behav ... | 2007 | 17683332 |
| microbial populations in acid mineral bioleaching systems of tong shankou copper mine, china. | to understand the composition and structure of microbial communities in different acid mineral bioleaching systems, and to present a more complete picture of microbially mediated acid mine drainage production. | 2007 | 17897227 |
| unique fluorophores in the dimeric archaeal histones hmfb and hpya1 reveal the impact of nonnative structure in a monomeric kinetic intermediate. | homodimeric archaeal histones and heterodimeric eukaryotic histones share a conserved structure but fold through different kinetic mechanisms, with a correlation between faster folding/association rates and the population of kinetic intermediates. wild-type hmfb (from methanothermus fervidus) has no intrinsic fluorophores; met35, which is tyr in hyperthermophilic archaeal histones such as hpya1 (from pyrococcus strain gb-3a), was mutated to tyr and trp. two tyr-to-trp mutants of hpya1 were also ... | 2008 | 18096639 |
| issues in the culture of the extremely thermophilic methanogen, methanothermus fervidus. | basic issues in the culture of the extremely thermophilic archaeon, methanothermus fervidus, have been investigated, including culture medium formulation, substrate yield and product yield coefficient, growth rate and stoichiometry, and h(2) uptake kinetics. the ph optimum for growth of this organism was estimated at 6.9. growth medium buffered with pipes instead of bicarbonate supported both increased growth rate and maximum biomass concentration. substitution of titanium(iii) citrate for the r ... | 1993 | 18601279 |
| the mode of cell wall growth in selected archaea is similar to the general mode of cell wall growth in bacteria as revealed by fluorescent dye analysis. | the surfaces of 8 bacterial and 23 archaeal species, including many hyperthermophilic archaea, could be stained using succinimidyl esters of fluorescent dyes. this allowed us for the first time to analyze the mode of cell wall growth in archaea by subculturing stained cells. the data obtained show that incorporation of new cell wall material in archaea follows the pattern observed for bacteria: in the coccoid species pyrococcus furiosus incorporation was in the region of septum formation while f ... | 2010 | 21169435 |
| complete genome sequence of methanothermus fervidus type strain (v24s). | methanothermus fervidus stetter 1982 is the type strain of the genus methanothermus. this hyperthermophilic genus is of a thought to be endemic in icelandic hot springs. m. fervidus was not only the first characterized organism with a maximal growth temperature (97°c) close to the boiling point of water, but also the first archaeon in which a detailed functional analysis of its histone protein was reported and the first one in which the function of 2,3-cyclodiphosphoglycerate in thermoadaptation ... | 2010 | 21304736 |