| symport h+/carbohydrate transport into acholeplasma laidlawii cells. | | 1978 | 26600 |
| size and shape of mycoplasma virus mv-lg-l 172 dna. | sedimentation of dna of mycoplasma virus mv-lg-l 172 in neutral and alkaline density gradients showed that the chromosome of this virus is most probably formed by a double-stranded circular dna molecule with a molecular weight of approx. 10(7) daltons. these results were confirmed by direct electron microscopy of the viral dna. gamma-irradiation of the virus caused in its dna single breaks on the average per each 66 ev of absorbed energy. virus survival depended exponentially on the irradiation ... | 1978 | 35941 |
| [regulatory mechanisms of membrane lipids--with reference to environmental adaptation (author's transl)]. | | 1979 | 41021 |
| demonstration by electron microscopy of intracellular virus in acholeplasma laidlawii infected with either mv-l3 or a similar but serologically distinct virus (bn1 virus). | cultures of acholeplasma laidlawii strain m1305/68 were inoculated with mycoplasmatales virus-laidlawii 3 (mv-l3) and examined by electron microscopy. particles resembling mv-l3 were observed both intra- and extracellularly in thin sections prepared from mv-l3 infected cultures, but not from uninfected cultures. similar particles were occasionally observed in uninoculated cultures of a. laidlawii strain bn1 cells, from which a virus (bn1 virus) was subsequently isolated. this virus was morpholog ... | 1979 | 84856 |
| effect of various culturing conditions of acholeplasma laidlawii on the yield and properties of the cells and cell membrane. | acholeplasma laidlawii strain b cell cultured in complex medium have an optimal temperature of growth at 31 degrees. maximal growth at this temperature is 80% higher than at 37 degrees. by stirring of the culture, the yield of the cell mass of 4 liter-cultures increases by 200%. with these modifications the yield of cell membranes increases 3--4 times with respect to that of static culturing at 37 degrees. membranes obtained from better aerated cultures have a lower flavin and carotenoid content ... | 1977 | 67754 |
| homogeneity of lipopolysaccharides from acholeplasma. | five methods were employed to determine the heterogeneity or homogeneity of lipopolysaccharides from four acholeplasmal species, acholeplasma axanthum, a. granularum, a. laidlawii, and a. modicum. a axanthum lipopolysaccharide behaved as a single component in all tests. a. granularum exhibited two components of identical composition and antigenic specificity. a. modicum lipopolysaccharide behaved as three components in two tests, but all three were similar in composition and identical serologica ... | 1977 | 67111 |
| keratitis and acholeplasma laidlawi. | | 1976 | 61420 |
| carbohydrate content and enzymic activites in the membrane of spiroplasma citri. | | 1977 | 142817 |
| [bidimensional immunoelectrophoretic study of the antigenic composition of the membrane in various mycoplasma strains]. | membrane antigenic composition of acheloplasma laidlawii pg9, a. granularum bts-39, and mycoplasma fermentans pg 18(g) was determined by means of bidimensional immunoelectrophoresis in the presence of sodium desoxycholate 0.5%. depending upon the mycoplasma species from which membranes were obtained, 7 to 15 antigens were evidenced. using sodium desoxycholate presents the advantage over non-ionic detergents to dissolve better the mycoplasmic membrane antigenic complexes. a comparative study of f ... | 1976 | 61061 |
| pathogenicity of mycoplasmas for arthropods. | | 1979 | 44614 |
| the pathogenicity of avian mycoplasmas. | based on literature data and own experiences the author gives an outlook about pathogenicity of avian mycoplasmas. in chickens and turkeys m. gallisepticum and m. synoviae (in addition to it m. meleagridis exclusively in turkeys) are the most important mycoplasmas producing respiratory disease, inflamation of synovial membranes and other lesions. their pathogenic effect is very much influenced by dose of agent, route of entry of microorganism, age of birds, virulence and tropism of organism as w ... | 1979 | 44612 |
| [glucose induced transport of h+, k+ and lactate in the cells of acholeplasma laidlawii]. | currents of h+ and k+ ions initiated under the effect of facultative anaerobe of a. laidlawii, valinomycin, nigericin and carbonyl cyanide chloride phenylhydrasone on the membrane are recorded by means of the system of cation sensitive electrodes. it is stated that during glucose transport a redistribution of h+ and k+ and lactate between the cellular content and the medium takes place. preincubation of cells with 3-o-methyl-d-glucose essentially decreases the effect of induced ion transport. it ... | 1979 | 35239 |
| mycoplasma contamination in cell culture of crohn's disease material. | | 1979 | 89521 |
| ultrastructural and cytochemical studies on anionic surface sites of mycoplasma membranes. | negatively charged, chemical groups on the surfaces of mycoplasma membranes were studied in the electron microscope by staining with positively charged ferric oxide hydrosols in the acetic acid (ai-reagent) or propanoic acid (pi-reagent), respectively. the technique of thin sectioning was used. with the ai-reagent, no significant binding of the ferric oxide particles was observed on the mycoplasma membranes. with the lipophilic pi-reagent, the membrane surfaces of m. mycoides subsp. capri, m. my ... | 1977 | 65876 |
| thermotropic transitions in biomembranes. | | 1976 | 182064 |
| complement-mediated killing of acholeplasma laidlawii by antibodies to various membrane components. | mycoplasmas are useful models for biochemical studies of the mechanism of complement-mediated killing by antibodies to various membrane components. the purpose of this study was to determine the membrane antigens involved in immune killing of acholeplasma laidlawii. antibodies to a. laidlawii membrane total lipids, glycolipids, and phospholipids could be induced in rabbits after injection of reaggregates of the purified lipids with mycoplasma hominis protein as the carrier. killing of a. laidlaw ... | 1976 | 61175 |
| recognition of different pools of phosphatidylglycerol in intact cells and isolated membranes of acholeplasma laidlawii by phospholipase a2. | phospholipase a2 (ec 3.1.1.4) from pig pancreas hydrolyzes phosphatidylglycerol in intact cells and isolated membranes of acholeplasma laidlawii. complete degradation of phosphatidylglycerol in intact cells at 37 degrees c does not result in lysis as shown by the retention of intracellular k+ ions and the cytoplasmic glucose-6-phosphatase, as well as the inability to detect activity of membrane-bound intracellular nadh-oxidase. a. laidlawii was grown on linoleic acid. phospholipase a2 treatment ... | 1977 | 191065 |
| rapid biochemical tests for characterization of the mycoplasmatales. | methods are described for the rapid detection of beta-d-glucosidase and phosphatase in mycoplasma cultures using fluorogenic 4-methylumbelliferone substrates. these methods were applied to a selection of mycoplasma cultures and were compared with the conventionally used tests for these enzymes. results were similar by both methods, but the fluorogenic tests could be read after 1 h, whereas the conventional tests took several days. | 1977 | 194917 |
| physiological role and membrane lipid modulation of the membrane-bound (mg2+, na+)-adenosine triphosphatase activity in acholeplasma laidlawii. | the membrane-bound adenosine triphosphatase (atpase) activity of acholeplasma laidlawii b differs in many respects from the common (mg2+, ca2+)-atpase activity of higher bacteria, most notably in that it is specifically activated by mg2+ and strongly and specifically stimulated by na+ (or li+). various inhibitors diminish the atpase activity with a concentration dependence which suggests that a single enzyme species is responsible for all of the observed atp hydrolytic activity (both basal and n ... | 1978 | 31351 |
| [experimental infection of dogs with myocplasmas (acholeplasma laidlawii)]. | | 1977 | 202113 |
| membrane-bound thioesterase activity in mycoplasmas. | thioesterase activity was found in all mycoplasmas tested. activity was highest in acholeplasma species, whereas most of the sterol-requiring mycoplasma species showed little activity. the thioesterase activity of acholoplasma laidlawii is confined to the cell membrane. the enzyme could not be released from the membrane by either low- or high-ionic-strength solutions, with or without ethylenediaminetetraacetic acid, nor solubilized by detergents. the enzyme has a general specificity for long-cha ... | 1977 | 14108 |
| microcalorimetric detection of growth of mycoplasmatales. | a static ampoule microcalorimeter was used to study the growth of mycoplasmas, acholeplasmas and ureaplasmas. growth as indicated by thermograms was compared with the results of conventional methods, namely, terminal dilution counts, plate counts, turbidimetric measurements, glucose consumption and ph changes. removal of oxygen had little effect on mycoplasma growth. the microcalorimetric method is potentially useful for identifying and enumerating the members of the mycoplasmatales. | 1976 | 11273 |
| the ayerst award lecture 1978. organization and dynamics of membrane lipids as determined by magnetic resonance spectroscopy. | | 1979 | 218704 |
| effect of ammonium ion concentration and osmotic pressure on growth of ureaplasma urealyticum (t-strain mycoplasma). | the effect of ammonium ion concentration and osmotic pressure on growth of ureaplasma urealyticum type viii was determined by using a well-buffered broth medium containing 10 mm urea. the addition of nh4cl to the medium at concentrations up to 10 mm did not affect growth; however, addition of larger quantities progressively decreased both the specific growth rate (mu) and the maximum yield of the culture, with concentrations of 80 mm completely inhibiting growth. addition of either 150 mm kcl or ... | 1978 | 26668 |
| characterization of the mycoplasma membrane proteins. v. release and localization of membrane-bound enzymes in acholeplasma laidlawii. | the peripheral membrane protein fraction released by washing acholeplasma laidlawii membranes with low-ionic strength buffers contained about 50% of the total membrane-bound ribonuclease and deoxyribonuclease activities. the atpase, nadh oxidase and p-nitrophenylphosphatase activities remained bound to the membrane even when edta was added to the wash fluids, and thus appear to belong to the integral membrane protein group. serving as a marker for peripheral membrane proteins, the membrane-bound ... | 1975 | 234252 |
| electron-microscopic study of a mycoplasmatales virus, strain mv-lg-ps2-l 172. | morphology and adsorption of a globular virus, lysing acholeplasma laidlawii were studied in ultrathin sections of plaques in a lawn of the host strain. the virus was globular, about 50 to 90 nm in diameter, with a clearly defined membrane, 6.5 to 8 nm thick. a protuberance about 25 to 35 nm long and 12 to 20 nm thick was observed on numerous virus particles. the evenly granulated, electron-optically dense content of the cells became clearer in cells affected by the viruses. fibrillar structures ... | 1975 | 234904 |
| reduced nicotinamide adenine dinucleotide oxidase activity in membranes and cytoplasm of acholeplasma laidlawii and mycoplasma mycoides subsp. capri. | the properties of the membrane-bound reduced nicotinamide adenine dinucleotide (nadh) oxidase of acholeplasma laidlawii were compared with those of the corresponding cytoplasmic activity of mycoplasma mycoides subsp. capri. the striking differences in ph optima, susceptibility to inhibitors and detergents, and heat inactivation between the nadh oxidase activity, with oxygen as an electron acceptor, and the nadh oxidoreductase activity, with dichlorophenol indophenol (dcpip) as an alternate elect ... | 1976 | 11208 |
| detection of serum proteins in the electrophoretic patterns of total proteins of mycoplasma cells. | the contamination of mycoplasma cell preparations by serum proteins originating from culture medium was studied. a. laidlawii and m. arthritidis cells were grown in the presence of 14c-aminoacids, and the cells were washed with 0-9% nac1 by threefold centrifugation. total proteins of the washed cells were analysed by sds gel electrophoresis. coomassie-stained electrophoretic patterns were compared with autoradiographs of the same gels. the stained electrophoretic pattern of washed a. laidlawii g ... | 1976 | 10333 |
| differential reaction of cell membrane phospholipids and proteins with chemical probes. | the major aims of this study were to determine the degree of phospholipid asymmetry and the neighbor analysis of phospholipids in different types of cell membranes. for this study a penetrating probe (fdnb), a non-penetrating probe (tnbs) and a cross-linking probe (dfdnb) were used. the reaction of hemoglobin, membrane protein and membrane pe and ps of erythrocytes with dfnb and tnbs was studied over a concentration range of 0.5 to 10 mm probe. tnbs reacts to an extremely small extend with hemog ... | 1976 | 7364 |
| cultivation of mycoplasmas in a modified tissue culture medium. | a new medium, which contained a chemically defined tissue culture base ("medium 199"), was developed for the cultivation of mycoplasmas. when supplemented with albumin, glucose, serum, and yeast extract, the new medium adequately supported the growth of mycoplasma and acholeplasma species. | 1976 | 7199 |
| surface balance study of the interaction between microorganisms and lipid monolayer at the air/water interface. | using the surface balance technique, we have compared the interaction between acholeplasma laidlawii and some marine bacteria towards different types of monolayered lipid films. cells from a. laidlawii and serratia marinorubra penetrate the film, whereas cells from psuedomonas fluorescens form a layer underneath the film. the forces that bind microorganisms to the air/water interface are not strong enough to scatter a condensed monolayer but increase the strength of loosely packed monolayers. | 1976 | 5058 |
| variation in distribution of membrane particles in acholeplasma laidlawii b with ph. | | 1976 | 4629 |
| properties of the 3-o-methyl-d-glucose transport system in acholeplasma laidlawii. | transport of 3-o-methyl-d-glucose (3-o-mg) by acholeplasma laidlawii cells was studied. the 3-o-mg transport system appeared to be constitutive in cells grown on 3-o-mg and glucose; the transport process depended on the concentration of substrate used and exhibited typical saturation kinetics, with an apparent km of 4.6 mum. 3-o-mg was transported as a free carbohydrate and was not metabolized further in the cell. dependence on ph and temperature and the results of efflux and "counterflow" exper ... | 1976 | 1368 |
| scanning of polyacrylamide gel electrophoresis columns for detection of unstained protein zones and for their localization relative to enzyme activities. | | 1975 | 236684 |
| crossed immunoelectrophoresis: polyacrylamide gel electrophoresis followed by electrophoresis into an agarose gel containing antibodies. | | 1975 | 236696 |
| osmotic behaviour of acholeplasma laidlawii b cells with membrane lipids in liquid-crystalline and gel state. | the osmotic behaviour of acholeplasma laidlawii b cells was investigated with combined spectrophotometric and enzymatic measurements. the conclusion could be drawn that this osmotic behaviour depends largely on the physical state of the membrane lipids. when part of the membrane lipids is in the liquid-crystalline phase the cell is able to swell and behaves as a good osmometer. however, when the membrane lipid is in the gel phase, the cell is unable to swell and the change in absorbance of the c ... | 1975 | 236790 |
| fractionation of membrane proteins by hydrophobic interaction chromatography and by chromatography on agarose equilibrated with a water-alcohol mixture of low or high ph. | | 1978 | 25906 |
| heterogeneity in the physical state of the exterior and interior regions of mycoplasma membrane lipids. | | 1975 | 238513 |
| activities of enzymes of purine and pyrimidine metabolism in nine mycoplasma species. | | 1979 | 232630 |
| in vitro antimycoplasmal activity of mepartricin. | the in vitro antimycoplasmal activity of mepartricin was evaluated on several mycoplasma strains. the results demonstrate that this polyene antibiotic possesses a high efficacy against these microorganisms. | 1979 | 118890 |
| transport properties of membrane vesicles from acholeplasma laidlawii. ii. kinetic characteristics and specificity of glucose transport system. | the glucose transport system of membrane vesicles isolated from acholeplasma laidlawii is saturable, with a km of 21.2 mum and v of 0.68 nmol min-1 (mg protein)-1. the process is ph-dependent and a break occurs in the arrhenius plot at 15 degrees c. exogenous substrates did not stimulate glucose transport probably due to their inability to penetrate into membrane vesicles. 3-o-methylglucose and 6-deoxyglucose competitively inhibited glucose transport. maltose inhibited transport of glucose nonco ... | 1975 | 286 |
| localization of enzymes in ureaplasma urealyticum (t-strain mycoplasma). | ureaplasma urealyticum cells were lysed by osmotic shock or by digitonin. the membrane fraction contained four to ten times as much protein as the cytoplasmic fraction. these values are in large excess of those reported for classical mycoplasmas, suggesting that the ureaplasma membrane fraction was heavily contaminated with proteins derived from the growth medium. the u. urealyticum urease activity was localized in the cytoplasmic fraction, whereas the adenosine triphosphatase activity was local ... | 1977 | 15980 |
| transport properties of membrane vesicles from acholeplasma laidlawii. i. isolation and general characteristics. | a new procedure for the isolation of membrane vesicles from acholeplasma laidlawii cells is described. the membrane vesicles are completely free from contaminations of whole cells and cell debris and represent a homogeneous fraction as shown by electron microscopy, ficoll density-gradient centrifugation, and titration on agar plate. absence of cytoplasmic contaminations was confirmed by double-labelling of membranes with 3h-oleic acid and 14c-uridine, as well as by distribution of specific marke ... | 1975 | 127739 |
| evaluation of an indirect immunoperoxidase test for identification of acholeplasma and mycoplasma. | | 1978 | 353224 |
| the nature and location of acholeplasma laidlawii membrane proteins investigated by two-dimensional gel electrophoresis. | the high resolution, two-dimensional electrophoresis system for the separation of proteins described by o'farrell, (o'farrell, p.h. (1975) j. biol. chem. 250, 4007--4021) has been modified for the separation of acholeplasma laidlawii proteins. reproducible protein patterns have been obtained from a. laidlawii cell, membrane and soluble protein preparations. the isoelectric focusing of membrane proteins was greatly improved by removing the bulk of the membrane lipid before solubilizing the protei ... | 1978 | 102346 |
| role of cholesterol in the action of cereolysin on membranes. | | 1978 | 102256 |
| membrane enzymes: artifacts in arrhenius plots due to temperature dependence of substrate-binding affinity. | for the membrane sodium-stimulated magnesium-adenosinetriphosphatase of acholeplasma laidlawii b both the vmax and km values in the michaelis equation very strongly with temperature. simulations of arrhenius plots show that an enzyme with a temperature-dependent km can yield a variety of arrhenius plot artifacts, most notably erroneous "breaks," if activity is assayed at a fixed substrate concentration. | 1978 | 146257 |
| the effect of high hydrostatic pressure on the membrane-bound atp-ase of acholeplasma laidlawii b [proceedings]. | | 1979 | 160925 |
| [infectious keratoconjunctivitis of cattle in ivory coast. i. microbiological aspects]. | | 1975 | 174165 |
| gliding mycoplasmas are inhibited by cytochalasin b and contain a polymerizable protein fraction. | studies are presented on the effect of cytochalasin b (cb) on the growth of five mycoplasma species, three acholeplasma species, and one spiroplasma species. the three gliding mycoplasma species (m gallisepticum, m pneumoniae and m pulmonis are the only mycoplasmas inhibited by cb. these are the only prokaryotes reported to be inhibited by cb. this suggested that these three mycoplasmas might have some sort of cytoskeletal structure. a protein fraction has been isolated from m gallisepticum whic ... | 1979 | 121144 |
| action of polymyxin b on bacterial membranes: phosphatidylglycerol- and cardiolipin-induced susceptibility to polymyxin b in acholeplasma laidlawii b. | to identify the polymyxin receptor molecules in the membranes of living microorganisms, fusion of intact acholeplasma laidlawii b with lipid vesicles was investigated according to the procedure of grant and mcconnell (1973). the naturally polymyxin-resistant a. laidlawii b was treated with phospholipid vesicles prepared from purified phospholipids of the polymyxin-susceptible salmonella typhimurium g30. a. laidlawii b absorbed between 15 and 45% of its own lipid content of the added tritium-labe ... | 1976 | 176930 |
| mechanisms of thermophily. | | 1978 | 365460 |
| the reduced nicotinamide adenine dinucleotide "oxidase" of acholeplasma laidlawii membranes. | an nadh dehydrogenase possessing a specific activity 3-5 times that of membrane-bound enzyme was obtained by extraction of acholeplasma laidlawii membranes with 9.0% ethanol at 43 degrees c. this dehydrogenase contained only trace amounts of iron (suggesting an uncoupled respiration), a flavin ratio of 1:2 fad to fmn and 30-40% lipid. its resistance to sedimentation is probably due to the high flotation density of the lipids. it efficiently utilized ferricyanide, menadione and dichlorophenol ind ... | 1976 | 177076 |
| structure of the mannoheptose-containing pentaglycosyldiacylglycerol from acholeplasma modicum. | | 1976 | 182247 |
| phosphorus nuclear magnetic resonance of acholeplasma laidlawii cell membranes and derived liposomes. | 1. the 129 mhz 31p-nmr spectrum of acholeplasma laidlawii membranes is very similar to the spectrum of the derived liposomes and is a typical "solid state" spectrum in which the major contribution to the linewidth is made by the chemical shift anisotropy. from the value of the chemical shift anisotropy an order parameter of 0.15 is estimated for the lipid phosphates in both membranes. 2. the 31p-nmr spectrum of the a. laidlawii membrane is insensitive to pronase digestion of 4-60% of the membran ... | 1976 | 174728 |
| acholeplasma laidlawii membranes: an electron spin resonance study of the influence on molecular order of fatty acid composition and cholesterol. | | 1978 | 216314 |
| purification and properties of acetate kinase from acholeplasma laidlawii. | acetate kinase (ec 2.7.2.1) was purified from acholeplasma laidlawii cytoplasm by a combination of ammonium sulfate fractionation, gel filtration, diethylaminoethyl-cellulose chromatography, and affinity chromatography on 8-(6-aminohexylamino)-adenosine 5'-triphosphate conjugated to sepharose 4b. the enzyme was composed of polypeptide chains of about 50,000 molecular weight as estimated from sodium dodecyl sulfate-polyacrylamide gel electrophoresis. under nondenaturating conditions, apparent mol ... | 1979 | 217865 |
| studies on classification of acholeplasmas isolated from horses, cattle and abattoir sewage. | | 1979 | 430937 |
| antibiotics for elimination of mycoplasmas and ureaplasma from bovine semen. | minocin at 500 micrograms/ml of semen extender eliminated ureaplasma from naturally or artificially infected bovine semen. minocin with lincospectin eliminated acholeplasma laidlawii, mycoplasma bovigenitalium, bovis, canadense, and arginini from artificially infected semen. stabilization times of 15 min at 35 c and 3 h at 4 c are important considerations to maximize antibiotic activity. | 1977 | 141466 |
| cholesterol transfer from serum lipoproteins to mycoplasma membranes. | | 1977 | 199249 |
| isolation of mycoplasmas from milk, lungs, and prepuce of cattle. | | 1979 | 398242 |
| differential fluorescence labelling with 5-dimethyl-aminonaphthalene-1-sulfonyl chloride of intact cells and isolated membranes in salmonella typhimurium and acholeplasma laidlawii. | for fluorescence labelling intact cells and isolated cell envelopes (membranes) from salmonella typhimurium and acholeplasma laidlawii were treated with mixed dansylchloride-lecithin-cholesterol vesicles. this kind of dansylation, which has been supposed to be specific for cell surface proteins, produced fluorescent protein pattern after sds-polyacrylamide gel electrophoresis only when isolated envelopes were dansylated. acid hydrolysis of fluorescent cell envelopes of salmonella typhimurium yie ... | 1975 | 235245 |
| [glucose transport into membrane vesicles from acholeplasma laidlawii cells]. | | 1975 | 235409 |
| purification of membrane proteins from acholeplasma laidlawii by agarose suspension electrophoresis in tween 20 and polyacrylamide and dextran gel electrophoresis in detergent-free media. | four of the membrane proteins from acholeplasma laidlawii that are soluble in the nonionic detergent tween 20 have been purified by preparative electrophoretic techniques utilizing different supporting media. the last purification step for two of the major proteins was a preparative polyacrylamide gel electrophoresis performed in the absence of any detergent. the proteins were recovered by continuous elution. the purity of the fractions was examined by analytical polyacrylamide gel electrophores ... | 1975 | 235514 |
| electron diffraction studies of biological membranes and lipids. | | 1975 | 235744 |
| isolation of acholeplasmas and mycoplasmas from aborted horse fetuses. | | 1979 | 473533 |
| replication of mycoplasmavirus mvl51: vi. acriflavine stimulates growth of this single-stranded dna virus. | | 1979 | 486185 |
| [identification of mycoplasmas isolated from monkeys]. | the authors identified 15 strains of mycoplasmae isolated from papio hamadryas suffering from leukemia and from healthy macacus rhesus, a green monkey and saimiri. a study was made of their biochemical properties, antigenic properties in the reaction of growth depression with immune sera to a number of standard strains, and also the protein composition by electrophoresis in polyacrylamide gel (epag). a number of mycoplasmae were identified as a. laidlawii, m. arthritidis--campo and m. cynomolgus ... | 1975 | 235821 |
| prevalence of mycoplasmal bovine mastitis in california. | seven species of mycoplasma plus one or more unknown species were found to cause bovine mastitis in california. both the frequency of cases and number of species of mycoplasma in samples received at the laboratory have increased from 1976 to 1978. by survey, nearly 4% of samples of bulk tank milk from dairy farms were found to contain mycoplasma of potential pathogenic significance. acholeplasma laidlawii was frequently isolated from samples both from cows and from farm bulk tanks during wet, ra ... | 1979 | 507492 |
| role of lipid fatty acyl composition and membrane fluidity in the resistance of acholeplasma laidlawii to complement-mediated killing. | | 1979 | 448140 |
| the properties of gel state lipid in membranes of acholeplasma laidlawii as observed by 2h nmr. | | 1979 | 437110 |
| is the vertical disposition of mycoplasma membrane proteins affected by membrane fluidity? | the influence of the physical state of the membrane lipid matrix on the vertical disposition of membrane proteins was studied with acholeplasma laidlawii. changes in membrane fluidity were brought about by altering the fatty acid composition of membrane lipids, by changing the growth temperature, by aging of cultures and by inducing changes in the membrane lipid-to-protein ratio through treatment with chloramphenicol. the lactoperoxidase-mediated iodination technique was used to label membrane p ... | 1979 | 444513 |
| adenine formation from adenosine by mycoplasmas: adenosine phosphorylase activity. | mammalian cells have enzymes to convert adenosine to inosine by deamination and inosine to hypoxanthine by phosphorolysis, but they do not possess the enzymes necessary to form the free base, adenine, from adenosine. mycoplasmas grown in broth or in cell cultures can produce adenine from adenosine. this activity was detected in a variety of mycoplasmatales, and the enzyme was shown to be adenosine phosphorylase. adenosine formation from adenine and ribose 1-phosphate, the reverse reaction of ade ... | 1975 | 236559 |
| ultrastructural localization of tellurite reduction in acholeplasma species. | aerobic reduction of tellurite by five acholeplasma species and two mycoplasma species was investigated by light and electron microscopy. among the acholeplasma species, colonies of a. laidlawii and a. oculi exhibited a heavy, macroscopically visible reduction of tellurite, whereas the reaction of a. axanthum was weaker. a. granularum and a. modicum did not reduce the substrate under the experimental conditions employed. the two subspecies of m. mycoides also reacted with tellurite, as did also ... | 1977 | 329638 |
| isolation of mycoplasmas and acholeplasmas from pneumonic lesions in sheep and goats in india. | | 1979 | 532484 |
| localization of a phosphoglycolipid in mycoplasma membranes using specific anti-lipid-antibodies. | a phosphoglycolipid, presumably identical to glyceryl-phosphoryldiglycosyl diglyceride, is the main component of the membrane glycolipids of mycoplasma mycoides subsp. capri. it is immunologically active. anti-phosphoglycolipid antibodies were induced in rabbits by intravenous injection of the flocculated complexes of methylated bovine serum albumin with a mixture of the phosphoglycolipid and the auxiliary lipids, phosphatidyl-choline and cholesterol. the specificities of the antibodies directed ... | 1977 | 337735 |
| immunogenetic analysis of the mechanism of induction of friend virus leukemia. | | 1975 | 236610 |
| unusual positional distribution of fatty acids in phosphatidylglycerol of sterol-requiring mycoplasmas. | | 1979 | 510549 |
| acid-soluble nucleotides of acholeplasma (mycoplasma) laidlawii a. | a study was made of the acid-soluble nucleotides present in acholeplasma (mycoplasma) laidlawii a during growth. the nucleotide pool of this organism is meagre, both qualitatively and quantitatively. the main nucleotides are uridine mono- and diphosphates, and none of the more complex nucleotide compounds, such as uridine diphosphate (udp) sugars or sugar peptides, required for cell wall formation, were detected. the nucleotide composition of a. laidlawii a is compared with that of the streptoco ... | 1978 | 358744 |
| [studies of mycoplasma mastitis in cattle. 5. studies of udder pathogenicity of mycoplasma and acholeplasma strains of different origins]. | isolated acholeplasma laidlawii strains exhibited highly differentiated behaviours regarding their udder pathogenicity. twelve of 16 tested strains were pathogenic to udder. symptoms of acute udder inflammation were caused by all ten a. laidlawii strains isolated from differentiated material of calf, but by only two of six strains isolated from differentiated material of cattle. intracisternal instillation of both strains from milk and one strain each from udder skin or cervical mucus caused mer ... | 1979 | 547917 |
| [studies of mycoplasma mastitis in cattle. 6. testing of mycoplasma isolated from milk for udder pathogenicity]. | strains of m. bovis, a. laidlawii, a. axanthum, and one unidentified strain of the family of mycoplasmataceae as well as altered secretion obtained from cows with mastitis were intracisternally applied in experiments and proved to be pathogenic to cattle udder. the results are likely to suggest the importance of mycoplasma isolated from the milk of mastitis cows to the aetiology of enzootic mastitis in three large dairy cattle stocks. intravenous application of m. bovis and a. laidlawii caused n ... | 1979 | 547918 |
| [studies of mycoplasma mastitis in cattle. 7. mycoplasma mastitis in 3 dairy cattle herds]. | reported in this paper is the occurrence of enzootic mastitis in three dairy cattle stocks. the outbreaks had been caused by mycoplasma bovis, acholeplasma laidlawii, acholeplasma axanthum as well as by one unidentified strain of the family of mycoplasmataceae. all animals with positive response to mycoplasma tests were identified an selected by repetitive testing of cultures in milk samples which had been taken from all lactating and dry cows and heifers as well as by evaluation of organ sample ... | 1979 | 547919 |
| [identification of acholeplasmas and mycoplasmas from aborted horse fetuses]. | | 1979 | 548033 |
| scanning electron microscopy of acholeplasma colonies on agar. | the colony formation and morphology of acholeplasma laidlawii and of an acholeplasma species was studied by scanning electron microscopy. in the colonies of both acholeplasma spp. large irregularly shaped cells, spherical cells, chains of beads, and long filaments with small bulbous distensions were seen. the membrane of some of the large cells seemed to be perforated, giving the cell a pitted appearance. | 1979 | 534959 |
| bristol laboratories division, bristol-myers company, syracuse, n.y. | | 1978 | 348086 |
| determination of membrane properties at the molecular level by carbon-13 and deuterium magnetic resonance. | | 1978 | 279301 |
| [acholeplasma laidlawii--a pathogenic or apathogenic species?]. | the question is discussed, whether acholeplasma laidlawii is a pathogenic or apathogenic species. studies have shown that it is a species of potential pathogenicity, however, with virulence properties differing greatly by strains. nothing so far has become known of contributive factors which might be needed for an infectious disease to develop. | 1979 | 551758 |
| heterogeneity among strains of mycoplasmatales virus-laidlawii 2. | five isolates of mycoplasmatales virus-laidlawii 2 (mv-l2) derived from bovine strains of acholeplasma laidlawii were shown to differ in host range, plaque morphology and neutralization tests with mv-l2 antiserum. cross-testing using virus resistant clones of a. laidlawii confirmed the heterogeneity of this group. adaptation of viruses to sub-optimal host was demonstrated. | 1977 | 560426 |
| simplified preparation of mycoplasmas, an acholeplasma, and a spiroplasma for scanning electron microscopy. | a simple, effective procedure was developed for scanning electron microscopic examination of mycoplasmas and similar organisms. cultivation of several mycoplasmal species, an acholeplasma, and a spiroplasma in broth media in leighton tubes with cover slips resulted in attachment of the organisms to the cover slips. the attached cells were easily processed for either scanning electron microscopy or light microscopy. by eliminating the need for centrifugation, which was used in previously describe ... | 1979 | 370106 |
| sensitivity of mycoplasmas of the respiratory tract of pigs and horses to erythromycin and its use in selective media. | the ability of erythromycin in liquid medium to suppress the growth of eight species of acholeplasma and of 13 species of mycoplasma was tested. the acholeplasma spp and two glycolytic mycoplasma spp from horses--a slow glucose-metabolising (sgm) mycoplasma and a strain n3, related to m mycoides--were sensitive to erythromycin. thus the growth of acholeplasmas can be suppressed when attempts are made to isolate pathogens from the porcine respiratory tract, but, in the case of horses, erythromyci ... | 1978 | 564541 |
| [comparative studies of avian mycoplasmas by flat gel polyacrylamide electrophoresis (author's transl)]. | the phenol-acetic-acid extraced cell proteins of mycoplasma (m.) and acholeplasma (a.) reference strains (pg31 (m. gallisepticum), pg 16 (m. gallinarum), pg30 (m iners), 17529 (m. meleagridis), wvu 1853 (m. synoviae), 1340 (m. anatis), pg8 and pg9 (a. laidlawii), ckk (serovar c), dd (serovar d), wr1 (serogroup f), 695 (serogroup i) and 694 (serogroup l) were anlysed by the flat gel polyacrylamide electrophoresis. with exception of pg8 and pg9 the coomassie blue-stained protein patterns show that ... | 1978 | 566006 |
| comparative studies between microbiological culture and uptake of uridine/uracil to detect mycoplasmal infection of cell cultures. | | 1979 | 376320 |
| [experience in identification and control of mycoplasma in dairy cattle stock under routine laboratory conditions]. | exacting demands have to be met by milk testing routine laboratories where mycoplasma species are to be cultured from milk samples. cleanliness of sampling, immediate cold storage, and no-delay transport of the milk samples to the testing centre are of greatest importance to the informative value of all mycoplasma tests. in the summer season, it is recommended to freeze the milk samples immediately after sampling. mycoplasma broth should not be inoculated to animals of the herd from which the sa ... | 1979 | 551756 |
| membrane lipid biosynthesis in acholeplasma laidlawii b: elongation of medium- and long-chain exogenous fatty acids in growing cells. | the chain elongation of a wide variety of exogenous fatty acids and the subsequent incorporation of the chain elongation products into the total membrane lipids of acholeplasma laidlawii b were systematically studied. within each chemical class of fatty acids examined, the extent of chain elongation increased with increases in chain length, reached a maximum value, and then declined with further increases in chain length. depending on chemical structure, exogenous fatty acids containing less tha ... | 1978 | 618849 |
| organometallic fatty acid and phospholipid analogs. synthesis and incorporation and detection in model membranes and biomembranes. | | 1978 | 620019 |
| [experience regarding the suitability of liquid and solid pplo culture media produced in gdr for mycoplasma cultivation]. | proper isolation of mycoplasma from milk or tissue was found to entail exacting demands on nutrient substrates. several institutes of the gdr have jointly developed and tested a medium which consists exclusively of gdr-made substances. it is a culturing medium which is suitable for detection and identification of acholeplasma and mycoplasma species in milk and organ samples taken from cattle. | 1979 | 551757 |
| [mycoplasma mastitis in cattle]. | reported in this paper are experiments from which the conclusion was drawn that acheloplasma laidlawii could cause changes in the mammary gland under certain circumstances. | 1979 | 551753 |
| inhibition of interferon induction in mice by mycoplasmas and mycoplasmal fractions. | | 1978 | 622377 |
| [experimental infection of mice, gerbils, and rats with mycoplasms from canine pericardium and cardial value (author's transl)]. | mycoplasms isolated from heart valves (a. laidlawii (a 42 hzbtl)) and pericardium (m. canis (a 56 hzkl)) of dogs were investigated for infectivity of rats, mice and gerbils (meriones unguiculatus). in mice the species m. spumans (pg 13), m. maculosum (pg 15), m. edwardii (pg 24) and m. molare (h 542) were investigated too. the mycoplasmas were inoculated by oral, subcutaneous, intravenous and intraperitoneal route. intraperitoneally the mycoplasmas were given without and with complete and incomp ... | 1977 | 595971 |
| [action of mycoplasma arthritidis and acholeplasma laidlawii on the immunological reactivity of balb/c mice infected with the rauscher leukemia virus]. | | 1979 | 389300 |