| [comparative studies of the suitability of different culture media for the isolation of mycoplasma californicum from milk samples]. | mycoplasma (m.) californicum und m. bovigenitalium mastitis had occurred in four herds. 240 milk samples of 120 cows were tested for m. californicum, using in parallel nine different culturing substrates and test methods. included were 120 initial and 120 final milk samples. inoculation of milk to medium-i broth containing v-factor yeast extract, incubation at 37 degrees c for 5 days, subsequent spreading onto cattle blood agar with staphylococcal nutrix, followed by 5 days of incubation proved ... | 1989 | 2619463 |
| antibiotic treatment of experimental mycoplasma californicum mastitis. | | 1989 | 2815518 |
| mycoplasma californicum mastitis in ewes as an experimental model for antibiotic treatment. | a strain of mycoplasma californicum successfully infected an experimentally inoculated ovine mammary gland causing a severe mastitis. the condition lasted for about 25 days, and resulted in atrophy and loss of milk production in the gland. four experimentally infected ewes, treated over a 3-day period with various regimes of the antibiotics oxytetracycline or tylosin during the acute stage of infection, successfully eliminated the infection. two others similarly treated with combined intramammar ... | 1987 | 3595752 |
| mycoplasma californicum mastitis in the dry dairy cow. | mastitis in 85 of 140 dry cows and 16 of 101 milking animals on one farm was shown to be caused by mycoplasma californicum. the infection was eradicated from the herd over a five month period by a combined programme of identification, segregation and culling of infected animals. some dry cows produced a self-cure, but the majority calved with nonfunctional quarters which produced very little colostrum and resulted in a high incidence of calf mortality. the source of the infection could not be es ... | 1986 | 3787992 |
| [cultivation of mycoplasma californicum and other mycoplasma species of cattle]. | | 1985 | 3888138 |
| further evidence of the involvement of mycoplasma californicum in bovine mastitis in europe. | | 1983 | 6879990 |
| isolation of mycoplasma californicum from an outbreak of bovine mastitis and the experimental reproduction of the disease. | a mycoplasma-like organism was isolated from five cows with chronic incurable mastitis from one dairy herd. this organism was shown to be mycoplasma californicum and was used to infect three cows by intramammary inoculation. the challenge organism was reisolated from all the infected quarters and the ensuing inflammatory response produced a chronic mastitis with a permanent drop in milk yield. antibiotic treatment in one animal was unsuccessful. | 1982 | 7112877 |
| the pathology of experimental mycoplasma californicum mastitis in ewes. | mastitis was reproduced experimentally by inoculating the lactating mammary glands of 12 ewes with mycoplasma californicum. large numbers of mycoplasmas were recovered from the milk. the mastitis had a lobular distribution and was characterized in the early stages by degeneration of the secretory epithelium and intense leucocytic infiltration of alveolar and ductal lumina. later there was widespread alveolar involution, and lymphohistiocytic infiltration and fibrosis of the interstitium. a monoc ... | 1993 | 8245231 |
| mastitis outbreak due to mycoplasma californicum and mycoplasma canadense in a commercial dairy herd in the state of jalisco, méxico. | a commercial dairy herd with 282 cows in lactation suddenly began to have a problem with atypical clinical mastitis. the first 28 cases showed severe purulent mastitis with hard swollen mammary glands, but without systemic signs of illness some of the cows were lame. the cases were not responsive to antibiotics. the evidence suggested that milking machine malfunction and other management practices may have contributed to spread infection and to increase the number of clinical cases. culture of m ... | 1999 | 10932757 |
| outbreak of pneumonia and arthritis in beef calves associated with mycoplasma bovis and mycoplasma californicum. | during an outbreak of pneumonia and arthritis in beef calves and young cattle on a large farm in north-west germany, mycoplasma bovis and mycoplasma californicum were isolated from tracheobronchial lavage fluids and synovial fluids. the microbiological findings in dead and living animals and the immunohistochemical demonstration of m californicum antigen in lung and arthritic joint tissue, indicated that under poor housing conditions and possibly other predisposing factors, this mycoplasma, like ... | 2002 | 12503789 |
| association between an outbreak strain causing mycoplasma bovis mastitis and its asymptomatic carriage in the herd: a case study from idaho, usa. | the objective of this study was to determine the association between mycoplasma mastitis and colonization of mycoplasma organisms at body sites of asymptomatic carriers. the investigation was done in a dairy herd with a first outbreak of mycoplasma mastitis. milk and swab solution specimens from accessible mucosal surfaces of body sites from cows and replacements were sampled at quarterly intervals (herd samplings 1-4). samples were cultured and mycoplasma spp. were isolated, speciated and finge ... | 2010 | 19880206 |
| molecular epidemiological analysis of mycoplasma bovis isolates from the pennsylvania animal diagnostic laboratory showing genetic diversity. | we have examined the genetic variability of mycoplasma bovis strains submitted to the pennsylvania animal diagnostics laboratory, university park (pa-adl), between december 2007 and december 2008. of 4,868 total samples submitted for mycoplasma testing, 302 were determined to be culture positive. mycoplasma bovis (63.6%), mycoplasma californicum (7.3%), mycoplasma bovirhinis (2.7%), mycoplasma bovigenitalium (0.7%), mycoplasma alkalescens (4.9%), mycoplasma putrefaciens (0.3%), and mycoplasma di ... | 2011 | 21426978 |
| validation of a mycoplasma molecular diagnostic test and distribution of mycoplasma species in bovine milk among new york state dairy farms. | mycoplasma mastitis is a contagious and costly disease of dairy cattle that significantly affects animal health and milk productivity. mycoplasma bovis is the most prevalent and invasive agent of mycoplasma mastitis in dairy cattle, and early detection is critical. other mycoplasma have been isolated from milk; however, the role and prevalence of these species as mastitis pathogens are poorly understood. routine screening of milk for mycoplasma by bacteriological culture is an important componen ... | 2016 | 27016831 |
| [manifestation of mycoplasma californicum in cattle organs]. | | 1986 | 3964013 |
| [virulence of mycoplasma californicum for the udder]. | | 1986 | 3964012 |
| [incidence of mycoplasma mastitis in czechoslovakia]. | by the mycoplasmic examination of 143 dairy cows exhibiting clinical signs of mastitis, mycoplasma californicum and m. bovigenitalium were detected as the causal agents of the disease at two localities. the occurrence of specific antibodies in milk, and/or blood serum, was characteristic of the course of the disease, as documented by the results of the examination of more than 300 samples. in some milk samples taken from cows suffering from mastitis of obscure etiology and obtained from state ve ... | 1985 | 3920807 |
| detection of bovine serum antibody specific to mycoplasma bovis and mycoplasma californicum by enzyme-linked immunosorbent assay (elisa). | elisa for use in epidemiologic field studies of bovine mastitis, were developed to measure serum antibody to mycoplasma bovis and m. californicum. varying levels of serological cross-reactivity to seven heterologous bovine mycoplasmal species were demonstrated in each assay. cross-reactivity was minimized by preincubation of cattle sera with suspensions of heterologous mycoplasma antigens, prior to measuring serum antibody to solid-phase antigen. heterologous absorption improved the immunologica ... | 1987 | 3667238 |
| enzyme-linked immunosorbent assay for detection of mycoplasma californicum-specific antibody in bovine serum: optimization of assay determinants and control of serologic cross-reactions. | an enzyme-linked immunosorbent assay (elisa) was adapted to detect mycoplasma californicum-specific antibodies in bovine serum. cross-reactive antibody was found in the m californicum-positive reference serum when assayed against each of 7 solid-phase antigens of heterologous mycoplasma species. cross-reactivity was further demonstrated by inhibition of elisa reactivity to m californicum solid-phase antigen by incubation of sera with antigen suspensions of each heterologous species. incubation o ... | 1987 | 3592357 |
| an antigen capture elisa test using monoclonal antibodies for the detection of mycoplasma californicum in milk. | the use of monoclonal antibodies to detect mycoplasma californicum was investigated in an antigen capture microtitre format. the finalized test was highly specific and no cross-reactions were detectable with any of the mastitis associated mycoplasma or bacterial antigens tested. using a concentration step involving centrifugation, the sensitivity of the test could be improved from 10(5)-10(7) to 10(3)-10(5) colony forming units per ml with pure broth cultures, and from 10(7) to at least 10(6) co ... | 1990 | 2396364 |
| complete genome sequence of the bovine mastitis pathogen mycoplasma californicum strain st-6t (atcc 33461t). | mycoplasma californicum is one of several mycoplasmal species associated with bovine mastitis. the complete genome sequence of 793,841 bp has been determined and annotated for the m. californicum st-6 type strain, providing a resource for the identification of surface antigens and putative pathoadaptive features. | 2014 | 24994797 |
| complete genome sequence of mycoplasma californicum strain haz160_1 from bovine mastitic milk in japan. | bovine mycoplasmal mastitis is spreading quickly among cows. it often leads to clinical mastitis outbreaks and often results in huge economic losses. mycoplasma californicum is an important causal species of bovine mastitis. presented here is the 799,088-bp complete genome sequence of m. californicum strain haz160_1, which was isolated in japan. | 2014 | 25013143 |
| molecular epidemiology of cases of mycoplasma californicum infection in japan. | bovine mastitis due to mycoplasma californicum is often accompanied by huge economic losses, and the disease spreads very quickly. an appropriate molecular epidemiological analysis is needed to prevent and control infectious disease, but molecular epidemiological analysis methods for m. californicum have not yet been reported. here we developed a combination of multiple-locus variable-number tandem repeat analysis (mlva) and pulsed-field gel electrophoresis (pfge) methods, which are common genot ... | 2014 | 25281385 |
| detection of mycoplasma canadense and mycoplasma californicum in dairy cattle from argentina. | different species of mycoplasma can affect bovine cattle, causing several diseases. pcr sequencing and further analysis of the 16s-23s rrna its region have shown a significant interspecies variability among mollicutes. sixteen suspected isolates of mycoplasma spp. obtained from milk samples from dairy herds were amplified (16s-23s rrna its region). fourteen out of those 16 suspected mycoplasma spp. isolates were pcr-positive. to confirm the identity of mycoplasma bovis, these 14 isolates were te ... | 2014 | 25011595 |
| A PCR assay and PCR-restriction fragment length polymorphism combination identifying the 3 primary Mycoplasma species causing mastitis. | The focus of the current research was to develop real-time PCR assays with improved sensitivity and the capacity to simultaneously speciate the 3 most common mycoplasma mastitis agents: Mycoplasma bovis, Mycoplasma californicum, and Mycoplasma bovigenitalium. Real-time PCR was chosen because it provides rapid results. Partial 16S rRNA gene sequencing was used as the gold standard for evaluating candidate real-time PCR assays. To ascertain the real-time PCR assay specificity, reference strains of ... | 2012 | 22192198 |
| mycoplasma mucosicanis sp. nov., isolated from the mucosa of dogs. | fourteen mycoplasma strains were isolated from the oral cavity and genital tract of asymptomatic dogs. isolates had been preliminarily identified by conventional serological testing as mycoplasma bovigenitalium, but in 16s-23s rrna intergenic spacer pcr-rflp assays the isolates exhibited an rflp pattern distinct from m. bovigenitalium pg11(t). analysis of the 16s rrna gene placed a representative of the isolates (strain 1642(t)) in the m. bovigenitalium subcluster of the mycoplasma bovis cluster ... | 2011 | 20418418 |
| short communication: the effect of centrifugation and resuspension on the recovery of mycoplasma species from milk. | low sensitivity of a single bulk tank milk culture is a major limitation for detection of mycoplasma organisms. we hypothesized that sedimentation of mycoplasma spp. in a milk sample by centrifugation followed by resuspension in a small volume of fluid before agar plating would increase the ability to detect mycoplasma spp. compared with direct conventional culture. the experiment was conducted to determine recovery of mycoplasma spp. from milk as affected by 1) treatment (centrifugation vs. con ... | 2009 | 19700705 |
| germicidal activities of representatives of five different teat dip classes against three bovine mycoplasma species using a modified excised teat model. | six representative teat dips from five different teat dip classes were tested for germicidal activity against challenge exposure to mycoplasma bovis, mycoplasma californicum, and mycoplasma bovigenitalium using a modified excised teat model. all teat dip formulations tested were efficacious against all of the mycoplasma species, providing bacterial logarithmic reductions above 4. the germicides performed best against m. bovigenitalium with an average log reduction (lr) of 6.29. average lr were 5 ... | 2002 | 12214982 |
| pasteurization of discard mycoplasma mastitic milk used to feed calves: thermal effects on various mycoplasma. | discard milk from sick or antibiotic-treated cows is often used as an economical alternative to milk replacer at dairy farms. this practice poses a health risk to calves if the discard milk is from cows with mycoplasma mastitis. mycoplasma bovis, mycoplasma californicum, and mycoplasma canadense are among the agents known to cause contagious mastitis in cattle and occasionally pneumonia, otitis media, or arthritis in calves. this report describes a recent outbreak of calf polyarthritis and respi ... | 2000 | 11049070 |