| [comparative study of rosette and plaque formation in rats infected with mycoplasma arthritidis and acholeplasma laidlawii]. | m. arthritidis suppresses rosette- and plaque-formation in rats infected with this strain of mycoplasma. on the 15th day the immune response was restored in the control and experimental groups of animals. in later stages a strong stimulation of rfc and pfc was recorded, which levelled on day 150. on the contrary, a. laidlawii stimulated rfc and pfc in all stages of infection. a relationship between the phenomena described and pathogenic properties of the mycoplasma is discussed. | 1979 | 380687 |
| [action of mycoplasma arthritidis and acholeplasma laidlawii on the immunological reactivity of balb/c mice infected with the rauscher leukemia virus]. | | 1979 | 389300 |
| binding of sodium [195au]aurothiomalate to mycoplasma arthritidis. | the binding of sodium [195au]aurothiomalate (atm) to whole cells of mycoplasma arthritidis has been measured within the temperature range of 4 to 53 degrees c. the time course (kinetics) and the effect of varying the total concentration of free atm in the suspension at 37 degrees c were also measured. the extent of binding at 37 degrees c leveled off after 30 min at approximately 65 nci of [195au]atm per mg of membrane protein. the amount of atm bound per cell appeared to be directly proportiona ... | 1977 | 409687 |
| [effect of mycoplasma arthritidis on mouse and rat lymphoid cells in experimental mycoplasma infection]. | early stages of mycoplasma infection of mice and rats were accompanied by suppression of the populations of rosette- and plaque-forming cells. later the character and dynamics of the immune response to m. arthritidis differed in mice and rats. in mice mycoplasma infection was accompanied by stimulation of rosette-forming cells with some suppression of the plaque-forming cells from the 7th to the 36th day of infection. in rats by the 7th day the number of plaque- and rosette-forming cells decreas ... | 1979 | 433504 |
| mycoplasma-dependent activation of normal lymphocytes: induction of a lymphocyte-mediated cytotoxicity for allogeneic and syngeneic mouse target cells. | mycoplasma arthritidis, m. hominis, and m. arginini were tested for their ability to induce a cytotoxic response from normal cba mouse lymphocytes against 51cr-labeled allogeneic target cells. in most cases, the mycoplasmas alone were not toxic for the target cells. furthermore, the mycoplasmas did not result in decreased lymphocyte viability but, in fact, contributed to enhanced lymphocyte survival. in the absence of normal cba lymphocytes, mycoplasmas alone did not induce a significant amount ... | 1977 | 562853 |
| mycoplasma-dependent activation of normal lymphocytes: role of arginine and nonviable mycoplasma antigen in the induction of lymphocyte-mediated cytotoxicity for syngeneic mouse target cells. | the ability of arginine supplementation to abrogate or potentiate mycoplasma arthritidis-mediated cytotoxicity induction by normal cba mouse lymphocytes was investigated. also, nonviable antigens of m. arthritidis were assayed for their ability to induce a cytotoxic response. the presence of additional arginine in the culture medium had no significant effect on the amount of cytotoxicity produced. in some cases, an increase in cytotoxicity was seen in the presence of additional arginine. amino a ... | 1977 | 562854 |
| arginine deiminase from mycoplasma arthritidis. properties of the enzyme from log phase cultures. | hydrodynamic, chemical, and optical properties of arginine deiminase (ec 3.5.3.6) from mycoplasma arthritidis are reported for the enzyme isolated from log phase cells. the s020,w and d020,w values of the enzyme are 5.48 s and 5.87 x 10(-7) cm3/s, respectively; the molecular weight is 87,300. determination of the amino acid composition shows that about 45% of the residues are nonpolar. another unique feature of the composition is the presence of 36 half-cystine residues. the state of oxidation o ... | 1978 | 681335 |
| arginine deiminase from mycoplasma arthritidis. structure-activity relationships among substrates and competitive inhibitors. | the arginine deiminase (l-arginine iminohydrolase, ec 3.5.3.6) from mycoplasma arthritidis catalyzes the irreversible hydrolysis of arginine and related guanidine derivatives to ammonia and the corresponding ureido analog of the substrate. the kinetic constants km, kcat, and kcat/km for the arginine deiminase-catalyzed hydrolysis of l-arginine are equal to 4 micron, 29 s-1, and 7.4 x 10(7) m-1 s-1, respectively, at 25 degrees c and ph 7.2. the enzyme also catalyzes the hydrolysis of l-canavanine ... | 1978 | 681336 |
| mycoplasma infection in pregnant rats: low viability of foetuses and newborn offspring. | the controversy regarding the possible role of genital mycoplasma infection in human reproductive failure gave rise to a study in which pregnant rats, previously shown to be of normal fertility, were inoculated 7-11 days after conception with either mycoplasma arthritidis or an equal volume of sterile broth medium. repeated observations during the course of pregnancy revealed several pathogenic effects. a statistically significant decrease was observed in the mean litter size of infected mothers ... | 1978 | 682177 |
| [structural study of mycoplasma by a scanning electron microscopy method]. | scanning electron microscopy was applied to the study of mycoplasma arthritidis, myc. gallisepticum, and acholeplasma laidlawii. small spherical elements (0.3--0.5 micrometer) formed a reticular "framework" of the colonies in which larger (1--1.5 micrometer) spheroid or irregular-shaped cells (in case of myc. gallisepticum) and also rod-shaped (1--3 micrometer), filamentous (3--5 micrometer) forms and "large bodies" (5 x 3--8 x 3.5 micrometer) were located. apparently the latter represented cong ... | 1978 | 747025 |
| animal models of arthritis. | the criteria for selecting and establishing a animal model for arthritis were described. rats are the most frequently used animals. adjuvant and myocobacterium induced arthritis provide a model of chronic joint inflammation, although significant differences exist when compared with human disease. a better model of arthritis in rats and mice can be induced by the injection of one strain of mycoplasma arthritidis. an even better model is presented by rabbits first immunized against a protein such ... | 1976 | 818450 |
| new models of chronic synovitis in rabbits induced by mycoplasmas: microbiological, histopathological, and immunological observations on rabbits injected with mycoplasma arthritidis and mycoplasma pulmonis. | a dose-dependent chronic synovitis was induced in rabbit knees after the intra-articular injection of both mycoplasma arthritidis and mycoplasma pulmonis. the inflammation progressed from an initial acute phase at 1 week characterized by edema, infiltration of the synovium with monocytes and heterophils, and desquamation of lining cells, to a more chronic phase at 1 and 3 months, in which villus hyperplasia, lymph "nodules," mononuclear cell infiltration, fibroplasia, and collagen deposition wer ... | 1977 | 873616 |
| chronic proliferative arthritis of mice induced by mycoplasma arthritidis: demonstration of a cell-mediated immune response to mycoplasma antigens in vitro. | lymphocytes taken from mice chronically infected with mycoplasma arthritidis exhibited a significant blastogenic response as measured by (3h)thymidine uptake when exposed in vitro to m. arthritidis antigens. the lymphocytes taken from 9 of 12 control mice of similar age exhibited an inhibition of (3h)thymidine uptake when exposed to m. arthritidis antigens. | 1975 | 1123254 |
| mycoplasma-mediated hyporeactivity to various interferon inducers. | three strains of mycoplasma arthritidis were shown to induce marked hyporeactivity in mice to interferon induction by both newcastle disease virus and poly(i:c). in contrast, the interferon response of mice to tilorone was only partially suppressed by pretreatment of the animals with mycoplasms. hyporeactivity to newcastle disease virus was maximal 1 and 3 days after mycoplasms treatment, but the interferon response was maximal 1 day after injection of the mycoplasmas and was no longer apparent ... | 1975 | 1205616 |
| the occurrence of mycoplasma arthritidis in the throat and middle ear of rats with chronic respiratory disease. | twenty-three rats with chronic respiratory disease were examined for the presence of mycoplasma arthritidis in the throat and middle ear. samples were taken at necropsy and swabbed onto an agar medium. morphologically typical colonies were subcultured and identified serologically. all of the rats showed evidence histopathologically of chronic respiratory disease, and typical m pulmonis colonies were isolated from 21 of 23. m arthritidis was isolated from 7 (30=) of the middle ears and 3 (13%) of ... | 1975 | 1207046 |
| hematologic changes in chronic arthritis of mice induced by mycoplasma arthritidis. | the arthritis caused by iv injection of m. arthritidis in mice was found to be associated with neutrophilia and lymphopenia without a change in the total wbc concentration. a mild anemia developed which was characterized by hypoferremia and plentiful re iron but with an increased plasma total iron-binding capacity. this anemia therefore differs from the anemia of chronic disorders and indeed from any anemia which occurs in man. | 1975 | 1208586 |
| role of arginine deiminase in growth of mycoplasma hominis. | arginine has been considered as the major energy source of nonglycolytic arginine-utilizing mycoplasmata. when three strains of mycoplasma arginini, and one strain each of mycoplasma arthritidis, mycoplasma fermentans, mycoplasma gallinarum, mycoplasma gallisepticum and mycoplasma hominis were grown in the medium with high arginine concentration (34 mm) compared with low arginine (4 mm), both the protein content of the organisms and the specific activity of arginine deiminase increased. m. ferme ... | 1976 | 1262306 |
| arthritis of mice induced by mycoplasma arthritidis. humoral antibody and lymphocyte responses of cba mice. | peak arthritis occurred 7 days after intravenous injection of cba mice with mycoplasma arthritidis and persisted in some animals for 84 days. a marked leucocytosis was apparent for the first 21 days. complement-fixing antibodies reached a peak 14 days after injection of the organisms and persisted at high levels for 84 days. metabolic-inhibiting and mycoplasmacidal antibodies were present but at much lower titres. | 1976 | 1275576 |
| human b cell differentiation induced by microbial superantigens: unselected peripheral blood lymphocytes secrete polyclonal immunoglobulin in response to mycoplasma arthritidis mitogen. | microbial superantigens (sa) activate a significant portion of the t cell repertoire based on their dual avidity for mhc class ii antigens and t cell receptor (tcr) epitopes common to products of one or several tcr beta chain variable gene families. while sa that induce massive t cell proliferation and cytokine secretion have been implicated in clinical syndromes characterized by shock and generalized immunosuppression, sa activation of a more restricted t cell response may also have significant ... | 1992 | 1299344 |
| mycoplasma arthritidis-derived superantigen. | | 1992 | 1418615 |
| induction of cytokines in human whole blood cultures by a mitogen derived from mycoplasma arthritidis and by staphylococcal enterotoxin b. | mycoplasma arthritidis produces a so-far only partially characterized soluble material (mas) that has a potent mitogenic effect on t lymphocytes of several species. similar to staphylococcal enterotoxins and a number of related toxins secreted by other species of bacteria, nanogram quantities of these so-called superantigens are sufficient to induce significant amounts of cytokines in the supernatant of lymphocyte cultures. induction of interleukin-6 (il-6) by mas in murine bone marrow-derived m ... | 1992 | 1490730 |
| protein and antigen variability among strains of mycoplasma arthritidis. | six strains of mycoplasma arthritidis isolated from different host tissues were examined for differences in their proteins and antigens by using one- and two-dimensional electrophoretic techniques as well as immunoblotting. one-dimensional electrophoresis revealed differences in concentrations of individual bands, but not differences in the overall banding pattern. by two-dimensional electrophoretic analysis, 25 proteins were identified as strain-variable whereas the majority of protein spots wa ... | 1992 | 1576410 |
| differential detection of mycoplasma pulmonis and mycoplasma arthritidis with species-specific dna probes. | mycoplasma pulmonis and mycoplasma arthritidis are both significant causes of infection in colonies of rodents, which are common experimental animals for biomedical research. since differential diagnosis has proven difficult due to similar homology between these two murine mycoplasma species, the development of a reliable identification system is of importance. in this study dna probes specific for m. pulmonis and m. arthritidis were generated after cross-hybridization between two reference stra ... | 1992 | 1643818 |
| an evolutionary conserved mechanism of t cell activation by microbial toxins. evidence for different affinities of t cell receptor-toxin interaction. | the enterotoxins produced by staphylococcus aureus are the most potent mitogens known. they belong to a group of distantly related mitogenic toxins that differ in other biologic activities. in this study we have compared the molecular mechanisms by which these mitogens activate human t lymphocytes. we used the staphylococcal enterotoxins a to e, the staphylococcal toxic shock syndrome toxin, the streptococcal erythrogenic toxins a and c (scarlet fever toxins, erythrogenic toxins (et)a, etc), and ... | 1991 | 1670601 |
| epitope-sharing between mycoplasma arthritidis and chondrocytes demonstrated by monoclonal antibodies. | polyclonal and monoclonal antibodies against mycoplasma (m.) arthritidis membranes were investigated in the indirect immunofluorescence test (iift) and enzyme immunoassay (eia) for their reactivity with rat and human chondrocytes as well as with rat skin fibroblasts. the monoclonal antibody a 79 gave positive reactions with rat chondrocytes in the iift up to a dilution off 1: 128 and in eia up to a dilution of 1:16. in the eia, the monoclonal antibodies a 31, a 32 and a 58 recognized m. arthriti ... | 1990 | 1701086 |
| induction of interleukin-6 in murine bone marrow-derived macrophages stimulated by the mycoplasma arthritidis mitogen mas. | cell-free supernatant of cultures from mycoplasma arthritidis (mas) functions as an extremely potent t-cell mitogen for human and murine lymphocytes. the t-cell response is dependent on the presence of accessory cells, presenting the intact e2 molecule on the cell surface. until now, pure mas protein has not been available. we developed a new multi-step method for mas purification. the main steps in this protocol are ammonium sulfate precipitation, anion exchange and hydroxyapatite chromatograph ... | 1990 | 1715197 |
| circulating and localized immune complexes in experimental mycoplasma-induced arthritis-associated ocular inflammation. | ocular deposits of immune complexes are believed to contribute to the anterior segment inflammations observed in association with the human arthritides. arthritis-related ocular inflammations may be reproduced in animals by infection with certain species of mycoplasma. to evaluate the role of immune complexes in the production of ocular lesions, we studied their involvement in the rodent model of experimental arthritis-associated ocular inflammation induced by mycoplasma arthritidis. sprague-daw ... | 1992 | 1730469 |
| the immunobiology of mycoplasma arthritidis and its superantigen mam. | | 1991 | 1802617 |
| the effect of mycoplasma arthritidis infection on the phagocytic activity of macrophages in rats and mice. | the phagocytic activity of mononuclear phagocytes of a/j mice and wistar rats was estimated by the carbon clearance test following injection of mycoplasma arthritidis. in mice, the overall phagocytic activity was significantly increased at the end of the first week (p less than 0.0001), but the increase was marginal by the third and fourth weeks after injection. a significant increase in the relative weight of liver and spleen was observed even when phagocytic activity had returned to levels sim ... | 1991 | 1878258 |
| effects of active and passive immunization in the mycoplasma arthritidis infection of rats. | viable, heat-inactivated, formalin-inactivated and sonicated mycoplasma (m.) arthritidis antigens as well as immune sera against m. arthritidis and spleen cells from lewis rats recovered from m. arthritidis infection were injected into naive lewis rats prior to a challenge infection with 10(7) cfu of m. arthritidis and tested for their protective effects. viable mycoplasmas induced arthritis combined with the production of high titers of antibodies against m. arthritidis and resistance to a seco ... | 1991 | 1930576 |
| klebsiella pneumoniae glycoprotein ru-41740 enhances resistance of mice against mycoplasma arthritidis-induced arthritis. | the effect of a purified glycoprotein extract from klebsiella pneumoniae with non-specific immunostimulating properties (ru 41740) on the development and course of mycoplasma arthritis was investigated. male a/j mice aged 2-3 months were given ru-41740 either intraperitoneally (i.p.) or orally prior to injection with mycoplasma arthritidis. ru-41740 injected i.p. at 0.1 mg kg-1 or given orally at 1 mg kg-1 prior to the infection and subsequently on alternate days enhanced the resistance of mice ... | 1991 | 1931133 |
| activation of mhc class i-restricted cd8+ ctl by microbial t cell mitogens. dependence upon mhc class ii expression of the target cells and v beta usage of the responder t cells. | the t cell response to microbial t cell mitogens (mtm) such as enterotoxins from staphylococcus aureus (se) and the soluble mitogen from mycoplasma arthritidis, resemble the minor lymphocyte stimulatory locus (mls) response in several aspects. an important feature of the mls response is it restriction to cd4+ cells. this study demonstrates that in contrast to mls, the mtm response includes both cd4+ and cd8+ subsets. both cd4+ and cd8+ cells expanded in il-2 after stimulation with seb showed pre ... | 1990 | 1968075 |
| [stimulation of the immune system by microbial "superantigens"]. | the enterotoxins and the toxic-shock-syndrome toxin-1 of staphylococcus aureus, the erythrogenic toxins of streptococcus pyogenes as well as a still uncharacterized exoprotein of mycoplasma arthritidis belong to a family of exotoxins, that have in common a potent mitogenic activity for t lymphocytes of several species. these proteins stimulate cd4(+)- and cd8+ t-lymphocytes by cross-linking the t-cell-antigen receptor with mhc-class-ii molecules on accessory or target cells. they are functionall ... | 1991 | 2060968 |
| strain differences in sensitivity of rats to mycoplasma arthritidis isr 1 infection are under multiple gene control. | at least 5 female rats from each of 24 inbred (aci, as, bdix, bh, bn, bs, buf, da, le, lew, mwf, om, sprd-cu3, w-krypt, and wky), rt1 congenic [bh.1l(lew), lew.1a(avn), lew.1c(wist), lew.1lv3(bh), lew.1k(shr), and lew.1n(bn)], and f1 hybrid [(lew x bn)f1, (lew.1w x lew.1a)f1, and (lew x lew.1w)f1] strains, representing eight independent major histocompatibility complex (mhc) haplotypes (a, b, c, dv1, k, l, n, and u) and five related rt1 haplotypes (av1, lv1, lv3, uv2, and uv3), were inoculated i ... | 1990 | 2111282 |
| stimulation of mouse lymphocytes by a mitogen derived from mycoplasma arthritidis (mam). viii. selective activation of t cells expressing distinct v beta t cell receptors from various strains of mice by the "superantigen" mam. | mycoplasma arthritidis t cell mitogen (mam), in association with its mhc ligand, is recognized by t cells that express tcr-alpha/beta assembled with a product(s) of the v beta 8 gene family. we show here that lymphocytes from mice which fail to express v beta 8 products can also be activated by mam and the resulting cultures exhibit a marked increase in v beta 6 tcr-bearing cells. evidence was also obtained that mam can activate t cells that express all three v beta 8 tcr. the mab, f23.1, which ... | 1990 | 2136890 |
| helper t cell-dependent human b cell differentiation mediated by a mycoplasmal superantigen bridge. | experimentally induced murine graft-vs.-host disease may be characterized by hypergammaglobulinemia, autoantibody formation, and immune complex-mediated organ system damage that mimics sle. these autoimmune phenomena are mediated by abnormal th-b cell cooperation, across mhc disparities, in which donor-derived allospecific th cells recognize and interact with mhc class ii antigens on the surface of recipient b cells. microbial toxins, termed superantigens, which bind to mhc class ii molecules an ... | 1990 | 2141059 |
| isolation of mycoplasma arthritidis from the joint fluid of boars. | during an outbreak of a disease in a swine insemination centre in bavaria, fed. rep. of germany, characterized by conjunctivitis, severe polyarthritis and infertility mycoplasmas have been isolated from the joint fluids of the three boars investigated. two of the isolate could be typed as mycoplasma (m.) arthritidis which causes arthritis in rats, mice and rabbits, the third as m. collis, a probably apathogenic rodent mycoplasma species. one of the two isolated m. arthritidis strains (strain d 2 ... | 1990 | 2220196 |
| comparison of four mycoplasma arthritidis strains by enzyme immunoassay, metabolism inhibition, one- and two-dimensional electrophoresis, and immunoblotting. | four mycoplasma arthritidis strains, two virulent (158p10p9 and 14124p10) and two avirulent (pg6 and h606), were examined for differences in their antigenic compositions. rabbit antisera prepared against each strain were compared for their reactivities against both homologous and heterologous strains by enzyme-linked immunosorbent assay and metabolism inhibition assay. these tests confirmed a close serologic relationship among the four strains. only by cross-absorbing each antiserum with intact ... | 1990 | 2229380 |
| interactions of cultured rat synovial and ocular ciliary body cells with two strains of mycoplasma arthritidis. | strains of mycoplasma arthritidis differ in their ability to cause joint and ocular inflammations. although the reasons for this difference are not fully understood, pathogenic mycoplasmas commonly require close associations with the cells they damage. using 3h-uridine labeled mycoplasma, we compared cellular interactions of in vitro cultivated rat synovial and ocular ciliary body epithelial cells with two american type culture collection strains of m. arthriditis shown to differ in their virule ... | 1990 | 2312496 |
| cloning and sequence analysis of the arginine deiminase gene from mycoplasma arginini. | arginine deiminase from mycoplasma arginini was purified. the purified enzyme has a molecular weight of 46,000 daltons as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. its specific activity (20 units/mg protein) and amino acid composition showed a strong similarity to that of the mycoplasma arthritidis arginine deiminase. the amino acid sequences of the n-terminal region and three internal peptides generated by enzymatic cleavage of the purified protein were determined ... | 1990 | 2325633 |
| pathogenetic mechanisms in the mycoplasma arthritidis polyarthritis of rats. | mycoplasma arthritidis is the causative agent of severe polyarthritis in rats and mice, which resembles human rheumatoid arthritis (ra). several mechanisms are involved in this disease. m. arthritidis releases substances acting on polymorphonuclear granulocytes (pmns), i.e. oxygen radical formation stimulating substances (500-3,000 daltons), a chemotactic substance (400 daltons) and an aggregating substance (500 daltons). these products were separated from the cell-free culture supernatant by ge ... | 1989 | 2481878 |
| enhanced interferon-gamma production by lymphocytes induced by a mitogen from mycoplasma arthritidis in patients with ankylosing spondylitis. | we have examined proliferation of and interferon-gamma (ifn-gamma) production by peripheral blood lymphocytes induced by a purified mitogen derived from mycoplasma arthritidis (mas) in patients with seronegative spondylo-arthropathies and healthy individuals. in all patients and healthy controls mas exerted a potent nonspecific lymphoproliferation. in contrast, only patients with ankylosing spondylitis (asp) showed a strong ifn-gamma production after stimulation with mas. the maximal ifn-gamma r ... | 1989 | 2510239 |
| the influence of social rank on the susceptibility of rats to mycoplasma arthritidis. | social ranks (a, o, rest) of adult male inbred rats housed in groups of four per cage were determined by repeated observations of their ejaculatory patterns under competitive conditions. once ranked, they were intravenously infected. fifteen clinical, hematological, and serological characters were continuously observed during 110 days. body weight reduction, arthritis score, and specific igg antibody titers were chosen as guideline characteristics and compared between different genotypes and soc ... | 1989 | 2614823 |
| experimental induction of arthritis in lew rats and antibody response to four mycoplasma arthritidis strains. | four mycoplasma arthritidis strains were examined for differences in virulence for lew rats and elicitation of antibody responses in the immunoglobulin (ig) m and g classes and in the four igg subclasses. two strains were highly arthritogenic and two were relatively avirulent. when the latter strains did induce arthritis, it was significantly less severe (p less than 0.05) and developed significantly later (p less than 0.001) than in rats injected with the two virulent strains, suggesting that t ... | 1989 | 2623796 |
| ph-controlled continuous cultivation of mycoplasmas. | the continuous cultivation of mycoplasmas in a ph-controlled metabolistat was investigated with the fermentative strain mycoplasma mobile 163k and the nonfermentative strain mycoplasma arthritidis isr1. the addition of medium and the removal of culture suspension were regulated by acid production from glucose by m. mobile 163k and by ammonium production from arginine by m. arthritidis isr1, respectively. for both strains the optimal ph for continuous growth was 7.0. the steady state could be mai ... | 1989 | 2729987 |
| cross reactivity of mycoplasma pulmonis and mycoplasma arthritidis antigen strains to anti-mycoplasma pulmonis antibody in the sera of mycoplasma pulmonis infected rats. | reactivity of mycoplasma pulmonis (mp) antigen strains to anti-mp antibody in the sera of mp infected rats was examined by enzyme linked immunosorbent assay (elisa). antibody titers to 7 kinds of mp antigens were measured in the sera of 20 mp isolated rats and 20 mp free rats by elisa and complement fixation test (cf test). elisa showed that there was no difference in the antibody titer of the same serum among 7 mp antigen strains employed, and the main cross reaction to anti-mp antibody took pl ... | 1989 | 2770128 |
| immunological reaction of rat tissue cells with antiserum against mycoplasma arthritidis membranes in indirect immunofluorescence test. | | 1988 | 3061250 |
| differential effects of in vitro gold sodium thiomalate on the stimulation of human peripheral blood mononuclear cells by mycoplasma arthritidis t cell mitogen, concanavalin a and phytohemagglutinin. | the effects of in vitro gold sodium thiomalate (gstm) on peripheral blood mononuclear cells (pbm) stimulated by a mycoplasma arthritidis t cell mitogen (mam), concanavalin a (con a), and phytohemagglutinin (pha) were compared in 5 individuals by measuring 3h-thymidine uptake by pbm. the mam induced lymphocyte transformations were more significantly inhibited by in vitro gstm than parallel assays using con a and pha. the effects could not be explained by differences in cell viability, length of c ... | 1986 | 3084780 |
| lymphocyte activation and induction of interferon gamma in human leucocyte cultures by the mitogen in mycoplasma arthritidis supernatant (mas). | this report succinctly summarizes a number of experiments in which human leucocytes were activated by the mitogen in the supernatant of cultured mycoplasma arthritidis (mas). lymphocytes from a large number of donors were found to be reactive when the two parameters of lymphoproliferation and of production of gamma interferon were analysed. lymphocytes of cord blood origin were also reactive in lymphoproliferation. their interferon production was low, but this has been established previously for ... | 1986 | 3097812 |
| recognition of mycoplasma arthritidis membrane antigens by rats and rabbits: comparison by immunoblotting and radioimmunoprecipitation. | sera from rats convalescing from infection with mycoplasma arthritidis were tested for their ability to react with m. arthritidis membrane antigens by immunoblotting and radioimmunoprecipitation. the absence of metabolism-inhibition (mi) antibody activity in these sera suggested that rats might fail to recognize those membrane antigens involved in eliciting mi antibodies therefore rabbit antisera, which are strongly mi positive for m. arthritidis, were used for comparison. antigenic recognition ... | 1988 | 3262951 |
| processing requirements for t cell activation by mycoplasma arthritidis-derived mitogen. | mycoplasma arthritidis produces in culture a polyclonal mitogen which is active for murine and human t lymphocytes in the presence of accessory cells (ac). we studied the requirements for processing and presentation by ac of mycoplasma arthritidis supernatant (mas) mitogen to human t cells. as inhibitors of ac processing, several agents were used which inhibit lysosomal function: the weak bases chloroquine and nh4cl, the cationic ionophore monensin and the competitive protease inhibitor leupepti ... | 1988 | 3265390 |
| the derrick edward award lecture. immunologic aspects of mycoplasma arthritidis-induced arthritis. | | 1987 | 3312092 |
| expression of metabolism-inhibition antibodies against mycoplasma arthritidis in rats. | shared antigens between mycoplasma arthritidis and rat tissues may be responsible for the lack of metabolism-inhibition (mi) and other neutralizing antibodies in rats with m arthritidis-induced arthritis. we were not able to confirm such antigens or to detect cross-reacting antigens between m arthritidis and rat lymphocytes, thymocytes, and muscle tissue. antisera of rabbit origin to rat lymphocytes, thymocytes, and skeletal muscle reacted by elisa with m arthritidis only when the mycoplasmal an ... | 1988 | 3354967 |
| [cytotoxic action of the individual membrane components of mycoplasma arthritidis and m. fermentans on rat lymphocytes]. | study of the toxic properties of the preparations obtained from m. arthritidis has revealed that the cytotoxic activity of m. arthritidis is mainly linked with the cytoplasmic membrane and, partially, with the cytoplasmic fraction. the membrane substances of m. fermentans and the products of its vital activity are toxic with respect to target cells, the component translocated into the culture medium consisting of globular proteins. interaction of the cytoplasmic membranes of these mycoplasma spe ... | 1988 | 3364084 |
| clonal analysis of human t cell activation by the mycoplasma arthritidis mitogen (mas). | mycoplasma arthritidis produces an as yet undefined soluble molecule (mas) that has a potent mitogenic effect on t cells of several species. we have used cloned human cytotoxic and proliferative t lymphocytes to dissect the molecular mechanism of t cell activation by this mitogen. reactivity to mas is clonally expressed among t cell receptor (tcr) alpha/beta chain-expressing t cell clones of cd4+ or cd8+ phenotype, as well as cd4-8- tcr alpha/beta chain-negative t lymphocyte clones expressing th ... | 1988 | 3264530 |
| stimulation of mouse lymphocytes by a mitogen derived from mycoplasma arthritidis. v. a small basic protein from culture supernatants is a potent t cell mitogen. | previous studies established that mycoplasma arthritidis produces a soluble t cell mitogen (mam), and that response of murine t cells to mam is genetically restricted. mam appeared predominantly in the supernatants of senescent cultures, but was not extracted in significant amounts from whole cells. a quantitative assay of mam activity was devised. mam formed noncovalent complexes with nucleic acids and uncharacterized high m.w. constituents of sera and of complex media. partially purified mam w ... | 1986 | 3489036 |
| immunologic analysis of mycoplasma arthritidis surface proteins. | lew rats experimentally infected with mycoplasma arthritidis failed to express metabolism inhibition (mi) antibodies. our hypothesis was that rats failed to immunologically recognize the antigens responsible for eliciting those antibodies. lew rats, injected i.v. with m. arthritidis, recognized only two low-mw surface antigens by radioimmunoprecipitation by 1 week after injection. however, by 6 weeks these rats recognized most of the same m. arthritidis surface antigens by western blot and radio ... | 1987 | 3499419 |
| studies on the mechanism of stimulation of t cells by the mycoplasma arthritidis-derived mitogen. role of class ii ie molecules. | a mitogen derived from the supernatant of broth cultures of mycoplasma arthritidis (mas-p) stimulates a proliferative response by normal, unprimed t cells and interleukin 2 production by some, but not all, t cell hybridomas. the response requires an ie-positive accessory cell (ac). the direct participation of ie, and not ia, in this system was confirmed by two sets of experiments. first, l cells transfected with ie, but not ia, provided effective ac function for both normal t cells and the t cel ... | 1987 | 3500207 |
| natural mycoplasmal infections in isolator-maintained lew/tru rats. | for 4 years a colony of cesarean-derived, isolator-maintained lew/tru rats was evaluated for mycoplasmal infection by serology, culture and histopathology. anti-mycoplasmal antibodies were detected by enzyme-linked immunosorbent assay (elisa), and the colony eventually was found to have inapparent infections of mycoplasma pulmonis and mycoplasma arthritidis. rats, naturally infected with m. pulmonis, remained consistently positive in the m. pulmonis elisa after their initial seroconversion, and ... | 1988 | 3263542 |
| indirect visualization of hematopoietic colony-forming stem cell (cfus) as a possible target cell for mycoplasma arthritidis. | utilizing specific rabbit antiserum against m. arthritidis together with complement, a portion of the marrow 7-day cfus population of cba mice infected with live mycoplasma organisms 1 day previously was shown to be inactivated. these cells might therefore be considered as candidate target cells for m. arthritidis. 11-day cfus were unaffected by similar treatment. | 1987 | 3569493 |
| activities of oxidative enzymes in mycoplasmas. | the activities of several oxidoreductases were measured in three fermentative and two nonfermentative mycoplasma species that were grown under aerobic or anaerobic conditions. acholeplasma laidlawii mg, mycoplasma hyorhinis gdl, and mycoplasma pneumoniae fh had very high apparent activities of pyruvate dehydrogenase and pyruvate dehydrogenase complex compared with the activities of mammalian fibroblasts or human platelet-enriched preparations, while mycoplasma salivarium vv and mycoplasma arthri ... | 1987 | 3571159 |
| [proliferation and splenic migration of mouse hematopoietic stem cells following a single injection of mycoplasma arthritidis]. | the effect of a single injection of live m. arthritidis microorganisms on the bone marrow and spleen cfu-s populations was assessed. one of the principal findings is that marrow cfu-s are recruited into cell cycle (as determined by hydroxyurea kill) early after m. arthritidis administration and stay in the cycle for at least 2 weeks thereafter. the peak level of cycling value (47%) was observed on day 4. the duration of increased cfu-s cycling activity was shown to coincide with a time period du ... | 1986 | 3730601 |
| arthritogenic effects of mycoplasma arthritidis t cell mitogen in rats. | the intraarticular injection of mycoplasma arthritidis t cell mitogen (mam) produced significant joint swelling with inflammation in da rats. histologic examination of the joint initially showed edema below the synovial surface layer with light polymorphonuclear and focal lymphoid cell infiltration. widespread but incomplete shedding of the surface layer of the synovial membrane and hypertrophy and hyperplasia of the subsynovium with fibroblasts and macrophages were observed subsequently. the hy ... | 1988 | 3262748 |
| [detection of mycoplasma antigens (mycoplasma arthritidis and mycoplasma fermentans) in the tissues of experimentally infected animals by using indirect immunofluorescence and aggregate-hemagglutination reactions]. | the analysis of the results obtained in the detection of mycoplasmic antigens in tissues of infected rabbits by means of the immunofluorescence test and the aggregate hemagglutination test, carried out in parallel, indicates that both these tests are highly specific, while the immunofluorescence test is more sensitive. | 1985 | 3887821 |
| decreased production of interferon alpha and interferon gamma in leucocyte cultures of schizophrenic patients. | in a first approach to measure the activity of the interferon system in schizophrenic patients, leucocyte cultures of schizophrenic patients and normal control individuals were set up using a whole blood assay. in this system both lymphoproliferation and the induction of interferon was tested. the lymphoproliferation (lp) test was performed with one bacterial recall antigen (ppd) and four different mitogens (phytohemagglutinin (pha), concanavalin a (con a), pokeweed mitogen (pwm) and a novel mit ... | 1985 | 3929565 |
| stimulation of mouse lymphocytes by a mitogen derived from mycoplasma arthritidis. vi. detection of a non-mhc gene(s) in the e alpha-bearing riiis mouse strain that is associated with a specific lack of t cell responses to the m. arthritidis soluble mitogen. | previous work using inbred, congenic and recombinant mouse strains showed a positive association with expression of e alpha and the ability of splenic cells to bind to and undergo proliferation in response to a t cell mitogen present in culture supernatants of mycoplasma arthritidis (mas). studies described in the present manuscript confirm this association because lymphocytes from mice expressing h-2a, h-2d, h-2j, h-2k, h-2p, h-2u, and h-2v all of which possess e alpha responded to mas, whereas ... | 1987 | 3110289 |
| characterization of the metabolism inhibition antigen of mycoplasma arthritidis. | the mycoplasma arthritidis antigen(s) responsible for eliciting metabolism-inhibiting antibodies in rabbits has been partially characterized. metabolism-inhibiting activity was absorbed from rabbit antisera by intact m. arthritidis cells and membranes but much less so by the soluble cytoplasmic fraction, indicating that the antigen is located on the outer membrane surface. it was stable to periodate and lipid extraction but labile to heat and proteolytic enzymes, indicating that it is protein in ... | 1985 | 4018874 |
| [detection of mycoplasma arthritidis and mycoplasma fermentans antibodies in rheumatoid arthritis patients by an immunoenzyme method]. | the optimum parameters of the immunoenzyme assay system for the identification of antibodies to m. arthritidis and m. fermentans in the sera of patients with rheumatoid arthritis have been established. the investigation has shown that the products obtained by the ultrasonic disintegration of the biomass of m. arthritidis and m. fermentans can be used as soluble antigens for adsorption on the polystyrene surface of plates. the use of the immunonenzyme assay, specially modified, has made it possib ... | 1985 | 4036415 |
| influence of genes of the major histocompatibility complex on ulcerative dermal necrosis induced in mice by mycoplasma arthritidis. | all mouse strains injected s.c. with mycoplasma arthritidis developed severe abscesses in the subdermal tissues. however, m. arthritidis strain 14124 p10 also induced an ulcerative dermal coagulation necrosis in mouse strains expressing the k and d haplotypes but not in those expressing the b, q, or s haplotypes. the use of inbred and congenic mouse strains established that the ulcerative necrosis was associated with the haplotypes expressed at the h2 major histocompatibility complex (mhc). the ... | 1985 | 4045222 |
| role of non-rt1 genes in the response of rat lymphocytes to mycoplasma arthritidis t cell mitogen, concanavalin a and phytohemagglutinin. | a comparison of splenic cells from various inbred rat strains indicated that da, lewis, buffalo, august, wistar furth, and (lew x bn)f1 all responded well to the mycoplasma arthritidis t cell mitogen, phytohemagglutinin and concanavalin a, but cells from bn and maxx rats were very weakly or nonresponsive. cells from congenic strains expressing nonresponder background genes, and responder haplotypes at rt1 (bn.1l(lew), rt1; bn.1a(da), rt1av1) failed to respond significantly to the mitogens. rats ... | 1986 | 3081642 |
| chronic arthritis of rabbits induced by mycoplasmas. iv. protective effect of immunization and prior infection. | rabbits exposed to mycoplasma arthritidis either by active immunization with killed mycoplasmas or by primary infection in the right knee were protected against intra-articular challenge with viable m. arthritidis in the left knee. this protection extended to the challenged knees of preinfected rabbits even while highly active inflammation persisted in the initially injected joints. we propose that the protection is mediated by antibody, probably metabolic-inhibiting antibody, present in the joi ... | 1985 | 4053440 |
| [co-leukemogenic activity of protein preparations, isolated from cells of mycoplasma arthritidis]. | | 1985 | 4082758 |
| [partial purification and properties of a mitogenic factor from mycoplasma arthritidis]. | m. arthritidis pg6 in a dose of 10(7) colony-forming units per ml was shown to produce a highly pronounced mitogenic effect, linked with a thermolabile factor, on the splenocytes of mice and rats. the mitogenic factor was absent in the lipid fraction and in the fraction formed by easily extractable mycoplasmal proteins. the supernatant fluid obtained after the sedimentation of m. arthritidis globular proteins with ammonium sulfate stimulated lymphocytes as actively as the intact culture of m. ar ... | 1985 | 4090817 |
| detection and characterization of defective mycoplasmacidal antibody produced by rodents against mycoplasma arthritidis. | a procedure has been developed for the detection of mycoplasmacidal antibody in the serum of rats and mice infected with mycoplasma arthritidis. antibody activity can apparently be measured only by using resting mycoplasma cells. the reaction is complement dependent and is usually complete within 3 h. early antibody (5 days) could be detected only within the immunoglobulin (ig)m fraction, whereas late antibody (42 days) is found within the igg fraction. the mycoplasmacidal antibody was highly sp ... | 1973 | 4125266 |
| characterization of mycoplasma strains from cats. | mycoplasma strains (b1, b2, cs, and s1a) were isolated from the saliva of normal cats. these were compared with a strain (co) isolated from the eye of a cat with severe conjunctivitis. on the basis of morphology, biochemical reactions, and antigenic composition, two distinct species were recognizable. strains co, b1, and b2 were antigenically unrelated to the other species tested; strains cs and s1a possessed antigenic components in common with mycoplasma arthritidis, m. salivarium, m. hominis, ... | 1967 | 4168314 |
| stable mycoplasma antigen preparations for indirect hemagglutination tests. | in immunological studies of mycoplasmas, the use of glutaraldehyde for the fixative makes it possible to use erythrocytes from commercially available defibrinated sheep blood. it eliminates the necessity of having to screen blood from individual sheep to obtain a suitable source of erythrocytes, as when employing tannic acid for fixation and sensitization. the chemical bonding of soluble mycoplasma proteins to glutaraladehyde-fixed sheep erythrocytes by bis-diazotized 3,3'dimethoxy derivative, b ... | 1974 | 4207279 |
| studies on proteolytic activity of mycoplasmas: the preparation and properties of gelatinolytic enzymes from strains of mycoplasma arthritidis. | | 1973 | 4356946 |
| induction of interferon in mice by mycoplasmas. | interferon was induced in mice after intraperitoneal inoculation with four different mycoplasmas. peak levels of between 100 and 300 u of interferon per ml were attained by 6 h postinfection with each of the mycoplasmas except mycoplasma arthritidis, which induced higher titers (400 to 11,800 u/ml) by this time. a fifth mycoplasma, m. pulmonis, induced interferon inconsistently and at a later (72 to 96 h) time. mycoplasmatales virus mvl51 and sterile mycoplasmal broth did not stimulate interfero ... | 1974 | 4373395 |
| serological reactions of mycoplasma hominis: differences among mycoplasmacidal, metabolic inhibition, and growth agglutination tests. | seven strains of mycoplasma hominis from human genital tracts were selected to produce antisera. the complement-dependent mycoplasmacidal (mc) activity and two complement-independent activities, metabolic inhibition (mi) and agglutination during growth (ga), of these sera were compared. the ga and mi tests were equivalent in titer and generally not affected by concentration of antigen; they revealed specific cross-reacting patterns among the seven strains. there was little if any cross-reactivit ... | 1974 | 4473426 |
| serological comparison of five arginine-utilizing mycoplasma species by two-dimensional immunoelectrophoresis. | five arginine-utilizing, nonglycolytic mycoplasma species were compared serologically by two dimensional immunoelectrophoresis. the survey included: mycoplasma hominis, mycoplasma arthritidis, mycoplasma gateae, mycoplasma gallinarum, and three strains of mycoplasma arginini. although the m. arginini strains showed strong cross-reactions, each strain produced a different antigenic profile which was distinct and which indicated significant antigenic differences between strains. the m. arginini st ... | 1974 | 4473427 |
| [factors influencing the susceptibility of mice to mycoplasma arthritidis]. | concentrated broth constituents markedly enhanced the development of abscesses after subcutaneous injection of mycoplasma arthritidis. under appropriate conditions, both virulent and avirulent strains of m. arthritidis produced toxic effects in mice, as detected by the development of necrotic abscesses and death of the animals. the treatment of mice at birth with m. arthritidis antigens rendered the animals more susceptible to infection with m. arthritidis later in life, as evidenced by increase ... | 1973 | 4572611 |
| interaction of mycoplasma arthritidis and other mycoplasmas with murine peritoneal macrophages. | neither mouse nor rat peritoneal macrophages were able to kill mycoplasma arthritidis to any observable degree in the absence of specific hyperimmune rabbit antiserum. although convalescent mouse and rat serum were somewhat inhibitory to m. arthritidis in the absence of macrophages, these sera did not promote active phagocytosis by peritoneal macrophages. in fact, the macrophages appeared to protect the mycoplasmas against the inhibitory effects of the antisera by stimulating their growth. hyper ... | 1973 | 4587519 |
| mycoplasma-associated immunosuppression: effect on hemagglutinin response to common antigens in rabbits. | a mycoplasma-associated immunosuppression of the primary and secondary hemagglutinin response to a common gram-negative antigen of escherichia coli (014) was demonstrated in the rabbit when preincubated mixtures of common gram-negative antigen and mycoplasma arthritidis membranes were injected intravenously. a similar immunosuppression was demonstrated only for the secondary hemagglutinin response to a common gram-positive antigen of staphylococcus aureus when preincubated mixtures of common gra ... | 1974 | 4593344 |
| fate of intravenously injected mycoplasma arthritidis in rodents and effect of vaccines. | virulent mycoplasma arthritidis strain 158 p10 was rapidly eliminated from the peripheral circulation of rats and mice during the first 2 h after intravenous injection of the organisms. characteristically after this time, the numbers of viable organisms remained remarkably constant through 48 to 72 h. the inability to clear the remaining mycoplasmas was not due to reticuloendothelial blockade but appeared to indicate the presence of a certain number of cells which were resistant to the clearance ... | 1973 | 4713693 |
| effects of salicylates on blood changes in mycoplasma arthritis in rats. | 1. the polyarthritis produced in rats by i.v. inoculation with mycoplasma arthritidis was made more severe by salicylates.2. the infection increased the erythrocyte sedimentation rate (esr), serum lysozyme, counts of total white blood corpuscles, polymorphonuclear cells and lymphocytes, haemolytic serum complement (ch 50) and its component c3. salicylates enhanced the rise in esr, ch 50 and c3, but suppressed the rise in lymphocytes and even induced a fall.3. salicylates did not interfere with t ... | 1973 | 4722043 |
| the effect of immunosuppression on polyarthritis induced by mycoplasma arthritidis in rats. | | 1973 | 4731989 |
| blood changes in experimental arthritis in two types of genetically different rats. | 1. rats genetically resistant to dextran and other agents producing the anaphylactoid reaction (nr rats), have a higher polymorph count than do rats which react to these agents (r rats).2. nr rats do not develop polyarthritis when a hind paw is injected intradermally with freund's adjuvant.3. the polyarthritis produced by an intravenous injection of mycoplasma arthritidis culture develops more slowly in nr rats than in r rats.4. it is not clear whether the higher polymorph count in nr rats is a ... | 1973 | 4788039 |
| proteolytic activity of mycoplasma arthritidis. | | 1972 | 5071646 |
| reproducible polyarthritis in rats caused by mycoplasma arthritidis. | | 1971 | 5090248 |
| chronic proliferative arthritis of mice induced by mycoplasma arthritidis. i. induction of disease and histopathological characteristics. | mice injected intravenously with mycoplasma arthritidis developed a chronic arthritis which persisted through at least 269 days. rats similarly treated developed an acute short-term infection. the mouse disease was characterized by periods of remission and exacerbation and was proliferative in nature, gradually spreading to adjacent joints. m. arthritidis strain 158 p10 was found to be less arthritogenic for mice than for rats, but further passage of this strain in mice substantially increased i ... | 1971 | 5154887 |
| chronic proliferative arthritis of mice induced by mycoplasma arthritidis. ii. serological responses of the lost and effect of vaccines. | complement-fixing antibodies were first detected in mice 7 days after intravenous injection with mycoplasma arthritidis. peak titers were observed at 21 days, and high levels of antibody persisted through 293 days. the metabolism-inhibiting antibody response was minimal. on fractionation of mouse sera, only 7s antibody was detected which first appeared at 12 days after injection and persisted throughout the experiment. in contrast, serum taken from rats injected with m. arthritidis contained pre ... | 1971 | 5154890 |
| non-traumatic blood collection from rat tail vessels. application in mycoplasma arthritidis. | | 1971 | 5158245 |
| immunological responses of the rat to mycoplasma arthritidis. | arthritis was produced in rats by the intravenous injection of mycoplasma arthritidis. metabolic inhibiting antibody and indirect hemagglutinating antibody could not be detected in the sera of arthritic or convalescent animals. nonmurine species of mycoplasma were capable of inducing metabolic inhibiting antibody in the rat. a hypothesis based upon the possible occurrence of heterogenetic antigens common to m. arthritidis and rat tissue was brought forward to explain these findings. complement-f ... | 1969 | 5305935 |
| a comparative study on the virulence of mycoplasma arthritidis and "mycoplasma hominis, type 2" strains in rats. | | 1967 | 6017752 |
| stimulation of mouse lymphocytes by a mitogen derived from mycoplasma arthritidis. vii. responsiveness is associated with expression of a product(s) of the v beta 8 gene family present on the t cell receptor alpha/beta for antigen. | mycoplasma arthritidis produces a potent soluble t lymphocyte mitogen (mam) which is dependent upon accessory cells bearing the alpha-chain of the i-e molecule (e alpha). lymphocytes from the riiis mouse strain which possess e alpha yet whose t cells fail to recognize the mam-e alpha complex were shown not to express v beta 8.1, 8.2 or 8.3 gene products present on the tcr-alpha/beta by virtue of their lack of reactivity with the f23.1 mab. because lymphocytes from the congenic b10.riii mouse str ... | 1989 | 2786024 |
| utilization of t cell hybridomas to establish that a soluble factor derived from mycoplasma arthritidis is truly a genetically restricted polyclonal t cell activator. | | 1983 | 6190931 |
| antigenic cross-reactions between mycoplasma arthritidis and rat tissues. | an antigenic relationship between mycoplasma arthritidis and rat tissues could be demonstrated using the enzyme-immune assay and the agar gel double diffusion test. cross-reactions were observed with joint homogenates as well as with muscle, lung, liver, kidney, spleen and brain tissue from rats. in addition, antibodies against joint homogenate were found in the sera of rats infected with m. arthritidis isr 1 using the indirect hemagglutination test. the significance of common antigens between m ... | 1984 | 6206639 |
| high-affinity binding of staphylococcal enterotoxins a and b to hla-dr. | staphylococcal enterotoxins a-e (refs 1-3), toxic shock toxin (tst-1) (ref. 1), a product of mycoplasma arthritidis and the mls antigens provoke dramatic t-cell responses. all are extremely potent polyclonal mitogens stimulating a large proportion of both murine and human cd4+ and cd8+t cells although activity is tightly restricted by major histocompatibility complex (mhc) class ii antigens. the murine t-cell response to staphylococcal enterotoxin b (seb) has recently been shown to involve only ... | 1989 | 2785644 |
| lymphoproliferative responses of spleen cells of inbred rat strains to mycoplasma arthritidis mitogen. | a potent mitogen for t lymphocytes of various species has recently been isolated from the supernatant of cultured mycoplasma arthritidis organisms (mas). in the mouse, reactivity to this mitogen has been observed to be controlled by the i-e subregion of the major histocompatibility complex. we have analysed the responses of spleen cells from several inbred rat strains covering practically all known haplotypes of the major histocompatibility complex of the rat (rt1). unlike in the mouse, all of t ... | 1984 | 6209789 |
| role of interleukin 1 in mycoplasma mitogen-induced proliferation of human t cells. | recently, a mitogenic effect of the supernatant of cultured mycoplasma arthritidis (mas) on human and murine lymphocytes has been described. here, we studied the role of accessory cells (ac) in mas-induced t cell proliferation in a system of human leukocytes. nylon-wool purified t cells were non-responsive to mas with regard to both proliferation and ifn-gamma production. the capacity of t lymphocytes to respond to mas could be restored when viable ac were added. treatment of ac with uv light re ... | 1989 | 2499531 |