| variation in the virulence of strains of mycoplasma pulmonis related to susceptibility to killing by macrophages in vivo. | the virulence of five strains of mycoplasma pulmonis, as judged by their ability to survive in the respiratory tract and induce pneumonia in cba mice, was related to the ability of viable organisms to persist in the peritoneal cavity. this appeared to be the result of differences in the ability of the strains to resist killing by peritoneal macrophages in vivo. it is suggested that resistance to phagocytosis by macrophages is an important determinant of virulence for m. pulmonis. | 1979 | 232125 |
| gliding motility of mycoplasma pulmonis. | the gliding movements of freshly isolated mycoplasma pulmonis cells were observed and measured. the motile cells had a characteristic appearance, an average speed of 0.4 to 0.7 micron/s, and a maximum speed of 1 micron/s. | 1977 | 263822 |
| detection of antibodies to mycoplasma pulmonis by an enzyme-linked immunosorbent assay. | the enzyme-linked immunosorbent assay, which entails the use of antigen-coated tubes and enzyme-labeled anti-immunoglobulins, was applied for the detection of antibodies against mycoplasma pulmonis in mice. a lysate of m. pulmonis was used as the antigen, and anti-mycoplasmal antibodies of the different immunoglobulin classes were detected by class-specific anti-immunoglobulin labeled with alkaline phosphatase. the optimal conditions for the test were determined, the specificity was evaluated, a ... | 1978 | 365742 |
| presence of viruses in a strain of mycoplasma pulmonis. | filtrates prepared from heavily grown agar cultures of m. pulmonis strain negroni-52 formed plaques on lawns of a. laidlawii strain jal but not on those of m. pulmonis strains ash or negroni-52. the plaque-forming agent proved to be rod-shaped particles morphologically identical with mycoplasmavirus group 1. evidence supporting the conclusion that the virus originated from negroni-52 has been obtained. electron microscopy revelaed that negroni-52 is also a carrier of long-tailed phage-like parti ... | 1979 | 477863 |
| the use of a simple barrier system to exclude murine pathogens. | a simple barrier system was used successfully to exclude mycoplasma pulmonis and sendai virus from a conventional mouse room. 49 weeks after introduction of the barrier system of management pasteurella pneumotropica was first isolated from animals in the room. | 1977 | 190466 |
| lung lysophospholipase activity in specific-pathogen-free rats infected with pasteurella pneumotropica or mycoplasma pulmonis. | the effects of pasteurella pneumotropica and mycoplasma pulmonis infections in specific-pathogen-free rats were studied to determine whether or not bacterial infections could cause an increase in rat lung lysophospholipase activity and/or changes in bone marrow eosinophil levels. lung lysophospholipase activity levels of m. pulmonis-infected rats were elevated with increasing infection dosages, but enzyme levels were not accompanied by a lung tissue eosinophilia or an increase in bone marrow eos ... | 1978 | 153332 |
| inhibition of mycoplasma-induced lymphocyte activation by sodium aurothiomalate. | sodium aurothiomalate, at concentrations of 10 to 150 microgram/ml of culture, inhibited rat lymphocyte stimulation by mycoplasma pulmonis mitogen in a dose-dependent manner. | 1978 | 101463 |
| pathological changes during aging in barrier-reared fischer 344 male rats. | pathology, microbiology, and selected serum chemistries were evaluated in 144 male fischer rats from 4 to 33 mo of age. the rats were reared and maintained under barrier conditions, which successfully excluded the introduction of major infectious disease agents throughout the entire study, including mycoplasma pulmonis. a wide variety of pathology was found and tabulated, and many lesions were found to increase in severity and incidence with age. there was a high correlation of renal disease sev ... | 1977 | 66246 |
| oxygen uptake and lung function in mice infected with streptococcus pneumoniae, influenza virus, or mycoplasma pulmonis. | model systems of respiratory infection in mice were established with streptococcus pneumoniae, influenza virus, and mycoplasma pulmonis. the lt50 for s. pneumoniae was 2 1/2 days, for lethal influenza 6 days, and for m. pulmonis 5 days. morbidity in sublethal influenza infections reached a peak during days 5 to 10, with recovery indicated by the third week. the course of each pulmonary infection was followed by use of the animal's maximal ability to consume oxygen (vo2max by determining the weig ... | 1978 | 23401 |
| the effect of pneumonia induced in mice with mycoplasma pulmonis on resistance to subsequent bacterial infection and the effect of a respiratory infection with sendai virus on the resistance of mice to mycoplasma pulmonis. | the effect of pneumonia induced by mycoplasma pulmonis in mice on the resistance of the lung to additional bacterial infection was examined. the effect of pneumonia induced by sendai virus on the resistance of mice to m. pulmonis was also investigated and compared with the effect of sendai virus on resistance to staphylococcus aureus. sendai virus infection decreased subsequent resistance to m. pulmonis in proportion to the virus dose. decreased resistance to subsequent s. aureus and m. pulmonis ... | 1978 | 215702 |
| strain difference of mouse in susceptibility to mycoplasma pulmonis infection. | | 1978 | 723103 |
| protective effect of vaccination against mycoplasma pulmonis respiratory disease in rats. | intravenous vaccination of rats with either viable or formalin-inactivated mycoplasma pulmonis reduced the incidence and severity of lower respiratory tract lesions after intranasal challenge with viable organisms. intranasal vaccination with killed organisms reduced the severity of rhinitis, but did not affect lesions in any other region of the respiratory tract. the maximum protection against upper tract lesions (rhinitis, otitis, and laryngotracheitis) was provided by intravenous immunization ... | 1978 | 711323 |
| effect of inherited deficiency of the fifth component of complement on arthritis induced in mice by mycoplasma pulmonis. | mycoplasma pulmonis inoculated parenterally into mice deficient in the fifth component of complement (c5) caused a chronic arthritis of significantly greater magnitude than in immunologically normal mice. during the chronic phase of arthritis m pulmonis organisms were isolated from the joints and organs of c5 deficient mice more frequently and in larger numbers than from immunologically normal mice. the implications of the results are discussed in relation to the pathogenesis of m pulmonis induc ... | 1978 | 697951 |
| comparative study of mycoplasma pulmonis and mycoplasma gallisepticum infections in turkey sinus. | infraorbital sinuses of young turkeys were injected with virulent strains of mycoplasma pulmonis and mycoplasma gallisepticum to compare the diseases caused by the 2 agents. mycoplasma pulmonis did not cause visible swelling from large quantities of mucous exudate in the sinuses, such as occurs with m gallisepticum, and it could not be recovered by bacteriologic culture technique after 3 weeks. however, slight exudate did accompany the m pulmonis infection. similarities between the disease caus ... | 1975 | 1163884 |
| effects of lymecycline on mycoplasma pulmonis-induced arthritis in mice. | the effect of lymecycline treatment on arthritis in c3h mice produced 5 months previously by i.v. inoculation of mycoplasma pulmonis was examined. treatment had little effect on the severity of the clinical disease. however, there was a marked reduction the severity of the histopathological inflammatory reaction in joints from lymecycline-treated mice when compared with untreated controls. this reduction was associated with eradication of viable mycoplasmas from the joints. the findings suggest ... | 1978 | 656320 |
| [serological examinations on natural infections with mouse pathogens in inbred mouse strains: difference in antibody detection among the strains (author's transl)]. | serological surveys on several infections were performed on the inbred mouse strains maintained at the central institute for experimental animals. in the first survey, 11 strains of mouse, which were 8 weeks of age or older and were kept in separate cages in the same animal room, were tested for antibodies to salmonella enteritidis, corynebacterium kutscheri, tyzzer's organisms, mycoplasma pulmonis, mouse hepatitis virus (mhv), sendai virus (hvj), pneumonia virus of mice (pvm) and minute virus o ... | 1978 | 631200 |
| [effect of ozone on mycoplasma pulmonis and bacterial aerols]. | | 1975 | 1240241 |
| lecithin changes in murine mycoplasma pulmonis respiratory infection. | we examined the lipid content of bronchoalveolar (ba) washes from both mice and rats infected with mycoplasma pulmonis, an etiological agent of murine pneumonia. during a 30-day period after intranasal inoculation, the total lipid content from infected and control rats (in milligrams per animal) remained relatively equal and unchanged. the saturated, unsaturated, and total lecithin contents in infected rats (in milligrams per animal) all increased. the maximum lecithin values were detected at 7 ... | 1979 | 582313 |
| lymphocyte activation by various mycoplasma strains and species. | the capacity of various mycoplasma strains and species to induce lymphocyte transformation in vitro was studied. of six strains of mycoplasma pulmonis studied, five displayed mitogenic activity with rat lymphocytes. among those m. pulmonis strains, our mp15 isolate and the negroni strain exhibited particularly potent mitogenic capacity. the murine mycoplasmas m. neurolyticum and m. arthritidis shared this mitogenicity for rat lymphocytes. however, the human mycoplasmas m. fermentans, m. pneumoni ... | 1977 | 562852 |
| mycoplasma-induced hydrocephalus in rats and hamsters. | mycoplasma pulmonis, a pathogen of the respiratory tract in rats, was inoculated intracerebrally into neonate rats and hamsters to determine if it would induce lesions in the ependyma. hydrocephalus was induced in 116 of 120 rats and in 23 of 28 hamsters. the severity of hydrocephalus was greater in the rats than in the hamsters. hydrocephalus induction occurred only subsequent to inoculation of viable m. pulmonis. at 2 weeks of age, rats became refractory to induction of hydrocephalus. light mi ... | 1977 | 558963 |
| effects of active and passive immunization on mycoplasma pulmonis-induced pneumonia in mice. | parenteral immunization of c3h mice with viable mycoplasma pulmonis organisms protected them from pneumonia induced by intranasal inoculation of these organisms. spleen cells obtained from immunized mice were ineffective in preventing syngeneic recipients from developint respiratory disease. in contrast, convalescent-phase serum enhanced the clearance of mycoplasmas from the respiratory tract of mice challenged with a small number of organisms. further, although 'immune' serum had no detectable ... | 1976 | 1278933 |
| protective effect of vaccines on mycoplasma pulmonis-induced respiratory disease of mice. | mice inoculated intranasally with either a virulent or an avirulent strain of live mycoplasma pulmonis were resistant to respiratory disease induced by a subsequent intranasal challenge with virulent organisms. similarly, mice inoculated intravenously with the virulent strain were resistant to intranasal challenge with the same strain. in contrast, mice inoculated intravenously with avirulent m. pulmonis were not resistant to intranasal challenge with the virulent mycoplasma strain. studies on m ... | 1977 | 558962 |
| protective effect of killed mycoplasma pulmonis vaccine against experimental infection in mice. | | 1977 | 558454 |
| the effect of mycoplasma pulmonis on fertilization and preimplantation development in vitro of mouse eggs. | the effect of mycoplasma pulmonis suspension on mouse on mouse fertilization and preimplantation development in vitro was examined. when sperm were preincubated with m. pulmonis, fertilization of eggs occured less consistently than when untreated sperm were used. there was also a highly significant and consistent reduction in embryonic develoment in the treated group, with relatively few embryos reaching the blastocyst stage. the adverse effects were not seen with m. fermentans or with m. pulmon ... | 1977 | 557421 |
| mycoplasma pulmonis arthritis in congenitally athymic (nude) mice. clinical and biological features. | congenitally athymic balb/ca nu/nu mice were employed to elucidate the role of the thymus in experimental mycoplasma pulmonis strain m53 infection, and nu/+ mice were used for comparison. chronic polyarthritis was frequently produced in both of nu/nu and nu/+ mice by intravenous injection of the organisms. macroscopically, nu/nu mice developed severer arthritis and a much lower grade of resolution than nu/+ mice. periarticular abscess, conjunctivitis, and emaciation were observed in some of the ... | 1979 | 530100 |
| solid-phase micro-radioimmunoassay to measure immunoglobulin class-specific antibody to mycoplasma pulmonis. | a solid-phase radioimmunoassay (ria) was used for the detection and quantitation of immunoglobulin class-specific antibodies to mycoplasma pulmonis in mouse sera. the ria was found to be more sensitive than the single radial haemolysis (srh) test for detecting antibodies in sera from mice at intervals after inoculation with m. pulmonis, since antibody was detected in 26 out of 29 serum samples by ria but in only 8 out of 29 samples by srh. a method is described for quantitating immunoglobulin cl ... | 1979 | 468375 |
| the development of extranodal lymphoid follicles in experimental bronchopneumonia. | extranodal formation of lymphoid follicles was morphologically studied in experimental bronchopneumonia. control gnotobiotic mice had no peribronchial lymphoid follicles and only lymphatic vessels were traced from the terminal bronchiolar region toward larger bronchi. during the week after intranasal inoculation of mycoplasma pulmonis, lymphoid follicles developed in the terminal portion of the lymphatics by the accumulation of small lymphocytes. a loose network of mesenchymal cells and early in ... | 1979 | 463554 |
| mitogenic activity of mycoplasma pulmonis. ii. studies on the biochemical nature of the mitogenic factor. | the mitogenic activity of mycoplasma pulmonis has been demonstrated to reside in the membrane of this microorganism. studies aimed at the identification of the membraneous mitogenic factor have revealed that membrane proteins are essential components of this mitogenic manifestation. in addition, it has been shown that the micro-organism's outer surface membrane proteins are responsible for mitogenic activity. it has been shown, however, that isolated membrane lipids are not mitogenic for rat ly ... | 1979 | 436928 |
| comparison of techniques for primary isolation of respiratory mycoplasma pulmonis from rats. | studies were performed to determine the optimal sites for primary isolation of mycoplasma pulmonis from the respiratory system of laboratory rats. in a preliminary study, a group of 42 rats was cultured for respiratory m. pulmonis at several sites. tracheal washes yielded the highest number of positive cultures (61.9%) and ground lung tissue yielded the lowest (19.0%), with the nasopharyngeal culture in the middle (52.38%). in a subsequent study, the tracheal wash was again the optimal method si ... | 1979 | 397930 |
| degenerate oligonucleotide primers for enzymatic amplification of reca sequences from gram-positive bacteria and mycoplasmas. | reca protein in gram-negative bacteria, especially in escherichia coli, has been extensively studied, but little is known about this key enzyme in other procaryotes. described here are degenerate oligonucleotide primers that have been used to amplify by the polymerase chain reaction (pcr) reca sequences from several gram-positive bacteria and mycoplasmas. the dna sequences of reca pcr products from streptococcus pyogenes, streptococcus mutans, enterococcus faecalis, and mycoplasma pulmonis were ... | 1992 | 1556091 |
| mitogenic activity of mycoplasma pulmonis. i. stimulation of rat b and t lymphocytes. | the mitogenic activity of mycoplasma pulmonis towards both rat b and t lymphocytes has been demonstrated. the data summarized in this report show that spleen cells obtained from t x bm rats were extensively activated by m. pulmonis. furthermore, m. pulmonis has been demonstrated to induce the development of antibody producing cells, as attested by the appearance of direct plaque forming cells against srbc and tnp-srbc in spleen cultures exposed to this mitogen. it was also demonstrated that rat ... | 1979 | 374251 |
| macrophage secretion and the complement cleavage product c3a in the pathogenesis of infections by mycoplasmas and l-forms of bacteria and in immunity to these organisms. | mouse peritoneal macrophages in culture exposed to mycoplasma pulmonis show marked biochemical changes. this micro-organism induces the release of hydrolytic enzymes from macrophages. the release is time- and dose-dependent and is not associated with loss of the cytoplasmic enzyme lactate dehydrogenase or any other sign of cell death. secretory products of macrophages may play a role in the pathogenesis of chronic inflammatory responses elicited by mycoplasma infections. one of the products of a ... | 1978 | 367651 |
| infection-induced airway fibrosis in two rat strains with differential susceptibility. | chronic infections play a significant role in the morbidity and mortality of patients with chronic airflow limitation. by stimulating airway inflammation, persistent infection has the potential to cause airway fibrosis. however, in patient this condition is most typically found in lungs damaged by other factors, such as smoking, abnormal secretions, or barotrauma. we report the characterization of mycoplasma pulmonis infection-induced lung fibrosis in two immunocompetent rat strains with no pree ... | 1992 | 1612760 |
| [seromonitoring of laboratory mouse and rat colonies for common murine pathogens (author's transl)]. | during a period from 1973 to 1978, 392 and 225 lots including 12,232 mouse and 8,044 rat individual sera, respectively, were examined for antibodies to murine hepatitis virus, sendai virus, bordetella bronchiseptica, mycoplasma pulmonis, tyzzer agents, salmonella typhimurium and corynebacterium kutscheri. of mouse lots 94.5% and 39.3% from breeder and user colonies, respectively, were negative for all antibodies examined as well as 31.6% and 17.2% of rat breeder and user colonies, respectively. ... | 1979 | 225183 |
| mycoplasma pulmonis infection with regard to embryo freezing and hysterectomy derivation. | eggs taken from the oviducts of mice, inoculated 3 weeks previously with mycoplasma pulmonis in the peritoneal cavity, were shown to be contaminated, and mycoplasmas were not eradicated by washing the eggs three times. zona-free eggs were also infected. embryos cultured for 24 hours in medium m16 were clear of infection. sperm from infected males was not significantly worse in the fertilization of uninfected eggs. | 1991 | 1667198 |
| localization of mycoplasma pulmonis in mice. | localization of mycoplasma pulmonis was examined in mice infected by direct contact with previously infected mice. after contact with infected animals, the organisms were shown to become detectable first in the nasal and oral cavities and trachea on the next day, and then they were recovered from the middle ear and brain after 3 and 4 days, respectively. after 7 days of contact, isolation rates retained to be 100% in the nasal, oral and tracheal cavities, while 95% in the middle ear and brain, 2 ... | 1976 | 1034561 |
| attachment and ingestion of mycoplasmas by mouse macrophages. i. kinetics of the interaction and effects on phagocyte glucose metabolism. | rates of attachment and ingestion of mycoplasma pulmonis by mouse peritoneal macrophages and the effect of the organism on phagocyte glucose metabolism were studied in vitro. antimycoplasma antibody, but not complement, enhances attachment of mycoplasma to macrophages. antibody-mediated attachment is not affected by the length of time the cell is maintained in culture or its adherence to a glass surface. adherence of mycoplasma by nonimmunologic means to cell spread on glass is significantly gre ... | 1977 | 851167 |
| attachment and ingestion of mycoplasmas by mouse macrophages. ii. scanning electron microscopic observations. | mycoplasmas adhere closely to the central region of the surface of mouse peritoneal macrophages in vitro. they do not appear connected to each other or the macrophage membrane, and they induce no change in the surface of the cell. after addition of antimycoplasma antibody, mycoplasmas show interconnections and the cell shows an increase occurrence of ruffled membrane and folding over the mycoplasmas. large and small lacunae appear in the membrane at sites other than those taking in organisms, an ... | 1977 | 851171 |
| new models of chronic synovitis in rabbits induced by mycoplasmas: microbiological, histopathological, and immunological observations on rabbits injected with mycoplasma arthritidis and mycoplasma pulmonis. | a dose-dependent chronic synovitis was induced in rabbit knees after the intra-articular injection of both mycoplasma arthritidis and mycoplasma pulmonis. the inflammation progressed from an initial acute phase at 1 week characterized by edema, infiltration of the synovium with monocytes and heterophils, and desquamation of lining cells, to a more chronic phase at 1 and 3 months, in which villus hyperplasia, lymph "nodules," mononuclear cell infiltration, fibroplasia, and collagen deposition wer ... | 1977 | 873616 |
| factors in resistance to and recovery from m. pulmonis-induced arthritis in mice. | a previous infection of c3h mice with viable mycoplasma pulmonis organisms protected them from arthritis induced by intravenous inoculation of these organisms, whether or not their initial arthritis had resolved at the time of challenge. although spleen cells obtained from such animals conferred resistance to acute arthritis in syngeneic recipient mice, convalescent-phase serum was much more effective in this respect. in addition, the transfer of 'immune' spleen cells to m. pulmonis-infected mic ... | 1977 | 879860 |
| mycoplasma pulmonis infection in gnotobiotic and conventional mice: aspects of pathogenicity including microbial enumeration and studies of tracheal involvement. | intranasal inoculation of mycoplasma pulmonis into gnotobiotic mice resulted in positive cultures from the anterior nares, nasopharynx, trachea, and lungs persisting for 21 da. similar inoculation of conventional, mycoplasma-free mice resulted in an increase in colony-forming units reaching a maximum by the 8th post-inoculation day and remaining constant in trachea and lungs through the 22nd day by an organ homogenate serial dilution method. all mice appeared ill with loss of total body weight, ... | 1976 | 940302 |
| self-mutilation in mice associated with otitis media. | mutilation of the external ear and adjacent tissues was an enzootic problem in outbred mcr:(icr) breeder mice in a commercial colony. necropsy examination of affected and randomly selected, nonaffected adult breeder mice revealed a probable association of the cutaneous lesions with suppurative otitis media. several microorganisms, including mycoplasma pulmonis and pasteurella pneumotropica, were isolated from the tympanic bullae. | 1975 | 1095819 |
| fetal wastage as a consequence of mycoplasma pulmonis infection in mice. | the effect of mycoplasma pulmonis, strain jb, on the outcome of pregnancy in to mice was studied. the mice were infected intravenously before or after mating and the fetuses were examined at autopsy just before parturition. an increase in the number of abnormal pregnancies was noted in mice infected about 2 weeks before mating, and there was a significant increase in the number of fetuses which died mid-way through pregnancy. mycoplasmas were not isolated from any of the fetuses although the org ... | 1975 | 1123815 |
| specific and nonspecific antibody responses in different segments of the respiratory tract in rats infected with mycoplasma pulmonis. | murine respiratory mycoplasmosis resulting from mycoplasma pulmonis infection in rats provides a useful model for the study of immunological and inflammatory mechanisms operative in the respiratory tract. we have previously shown that lew rats develop more severe disease than do f344 rats. to further study the production of antibody responses in chronic respiratory disease due to m. pulmonis infection, we examined the distribution and development of m. pulmonis-specific antibody-forming cells (a ... | 1991 | 1894371 |
| susceptibility of newly established mouse strain mps to mycoplasma pneumoniae infection. | a newly established mouse strain, mps, which is more sensitive to mycoplasma pulmonis than icr, ddy and other mouse strains was examined for its susceptibility to mycoplasma pneumoniae. in experimental infections with m. pneumoniae, it was observed that m. pneumoniae attached to tracheas of mps mice, and m. pneumoniae cells were isolated from tracheas and lungs of mps mice even after four weeks of infection, while no mycoplasmas were isolated from icr and ddy mice after one week of infection. sp ... | 1991 | 1908041 |
| arthritis of mice induced by mycoplasma pulmonis: humoral antibody and lymphocyte responses of cba mice. | peak arthritis occurred 14 days after intravenous injection of mycoplasma pulmonis and persisted in some mice at low levels for 84 days. a marked lymphocytosis occurred during the first week of infection with only a slight increase in polymorphonuclear leukocytes. complement-fixing antibodies appeared in low titer 3 days after infection and moderate levels persisted for 84 days. the metabolic-inhibiting and mycoplasmacidal antibody responses were absent or minimal. m. pulmonis appeared to be mi ... | 1975 | 1193724 |
| the survival of mycoplasma pulmonis in drinking water and in other materials. | | 1975 | 1210815 |
| arthritis in mice due to infection with mycoplasma pulmonis. ii. serological and histological features. | histological and serological data were obtained from a group of 160 mice given intravenous inoculations of 3.5 x 10(7) colony-forming units of mycoplasma pulmonis and studied for 46 weeks. the basic pathology was that of an initial acute inflammatory response within the joint and periarticular tissues, slowly changing to a chronic inflammatory response seen predominantly in subsynovial areas with marked synovial proliferation. occasional lymphoid follicles and plasma cells were seen in the subsy ... | 1976 | 1245761 |
| immunoglobulin class- and subclass-specific responses to mycoplasma pulmonis in sera and secretions of naturally infected sprague-dawley female rats. | mycoplasma pulmonis causes chronic murine respiratory mycoplasmosis and genital disease in rats. specific immunoglobulin m (igm), iga, and igg and its subclasses present in sera and tracheal and uterine lavage samples from 36 naturally infected sprague-dawley female rats were tested for reactivity with m. pulmonis in an enzyme-linked immunosorbent assay. ten specific-pathogen-free sprague-dawley female rats served as the negative controls. tracheal and uterine lavage samples were cultured quanti ... | 1991 | 2037378 |
| effects of mycoplasma pulmonis infection and exposure to nitrogen dioxide on activities of natural killer cells and macrophages of the mouse. | a marked enhancement was seen in activities of mouse splenic natural killer (nk) cells and macrophages one week after infection with mycoplasma pulmonis (mp) or exposure to nitrogen dioxide (no2). macrophage activity of the mp-infected mouse showed further augmentation by exposure to no2 while nk activity of mp-infected mice was not affected by no2-exposure. | 1991 | 2044674 |
| plaque-forming factor produced by mycoplasma pulmonis. | | 1976 | 1246349 |
| enhancement of natural and experimental respiratory mycoplasmosis in rats by hexamethylphosphoramide. | hexamethylphosphoramide (hmpa) was given orally (100 mg/kg/day) to: a) conventional rats of sprague-dawley and long-evans substrains known to have indigenous mycoplasma pulmonis infection, b) uninfected pathogen-free (pf) fischer rats, and c) pf and axenic fischer rats inoculated intranasally with m. pulmonis strains having a wide range of virulence. treated rats infected with virulent m. pulmonis, either naturally or experimentally, developed severe clinical signs of murine respiratory mycoplas ... | 1976 | 1247084 |
| expression of peroxidase activity in rat tracheal epithelial cells associated with mycoplasma pulmonis. | endogenous peroxidase activity has not been localized in the tracheal mucosal epithelial cells of specific pathogen-free (spf) rats. after natural infection with mycoplasma pulmonis in spf rats, peroxidase activity became localized to the endoplasmic reticulum, nuclear envelope, and golgi apparatus of tracheal ciliated or mucous secretory cells. some secretory cells occasionally had peroxidase-positive secretory granules. at 1 wk m. pulmonis was found to attach to these epithelial cells, which t ... | 1992 | 1310227 |
| elimination of mycoplasmas from the murine genital tract by hormone treatment. | twenty progesterone-treated to mice were infected intravaginally with mycoplasma pulmonis. one month later, ten of the mice were given oestradiol which changed the reproductive cycle to the oestrous phase and within a week resulted in eradication of the organisms from six of them; vaginal persistence in the other mice may have been due, at least in part, to reinoculation of organisms from the oropharynx, a site heavily infected in all the mice. the majority of the mice not treated with oestradio ... | 1990 | 2143480 |
| the immunotoxicity of tributyltin oxide (tbto) does not increase the susceptibility of rats to experimental respiratory infection. | 1. the dietary exposure of rats to tributyltin oxide at a concentration of 150 ppm for 6 weeks is known to lead to a significant reduction in relative thymic weight. 2. to determine whether this reduction in thymic weight also leads to an impairment of function sufficient to alter the host response to micro-organisms, we have examined the development of virus- and mycoplasma-induced pneumonia in tbto-exposed rats. 3. using a quantitative histopathological method for measuring both the extent and ... | 1992 | 1349222 |
| high-frequency rearrangements in the chromosome of mycoplasma pulmonis correlate with phenotypic switching. | mycoplasma pulmonis is a murine pathogen that causes chronic respiratory disease in laboratory rats and mice. several examples of high-frequency phenotypic switching have been reported for m. pulmonis, the molecular basis of which is unknown. we report here that during growth the m. pulmonis chromosome undergoes dna rearrangements at a high frequency. some of the rearrangements we examined correlated with changes in the susceptibility of the cells to mycoplasma virus p1, an example of phenotypic ... | 1992 | 1350316 |
| effects of aging on exocrine immune responses to mycoplasma pulmonis. | formalinized mycoplasma pulmonis was used to immunize 3 different age groups of fischer 344 rats. a specific antibody to this antigen was detected in both saliva and lung lavage fluids and differences were noted in the elicitation of secretory antibody between the different ages of the animals. few statistical differences were noted between the three age groups for salivary igg responses to m. pulmonis, regardless of the dosage given, even though all responses were greater than their respective ... | 1992 | 1365840 |
| infections intensify neurogenic plasma extravasation in the airway mucosa. | stimulation of sensory nerves in the airway mucosa of the rat evokes the release of inflammatory peptides such as substance p, which can increase microvascular permeability, resulting in a phenomenon known as neurogenic plasma extravasation. the change in vascular permeability is mediated by nk-1 receptors and is caused by the formation of gaps between endothelial cells of postcapillary venules and small collecting venules, which are the same vessels as are affected by inflammatory mediators suc ... | 1992 | 1443906 |
| pulmonary clearance of mycoplasma pulmonis in rats with respiratory viral infections or of susceptible genotype. | we sought to determine whether or not increased severity of bronchopulmonary disease due to mycoplasma pulmonis infection in rats with respiratory viral infections and in rats of susceptible genotype could result from altered pulmonary clearance. pathogen-free rats were exposed to aerosols of radiolabeled m. pulmonis and the numbers of m. pulmonis colony-forming units, and amounts of radiolabel in the lungs were determined immediately after exposure or 4 hours later. intrapulmonary killing of m. ... | 1992 | 1460843 |
| [differential diagnosis between acute rejection and infection after lung transplantation in rats]. | infiltration of mononuclear cell subpopulations was analyzed immunohistochemically in acute lung allograft rejection (bn/lew) and mycoplasma pulmonis bronchopneumonia in inbred rats. in lung allograft rejection, marked infiltration of activated macrophages, moderate infiltration of helper and suppressor t lymphocytes, and slight or moderate infiltration of b lymphocytes were observed around bronchioles and vessels, as a thick layer of cells like concentric circles, forming a dense cuff. in acute ... | 1992 | 1474689 |
| the influence of genotypes and social ranks in the clinical course of an experimental infection with mycoplasma pulmonis (mrm) in inbred rats. | social ranks of healthy, adult male inbred rats housed in groups of four per cage were determined by their relative differences in intromission and ejaculatory patterns observed repeatedly under competitive copulatory conditions. once ranked they were inoculated with m. pulmonis by contact with castrated females suffering clinically from m.pulmonis.--body weight reduction and scores for laryngo-bronchotracheal breathing sounds and dispnoea or sneeze or wipe and the titers of specific antibodies ... | 1992 | 1492524 |
| protein and antigen variability among strains of mycoplasma arthritidis. | six strains of mycoplasma arthritidis isolated from different host tissues were examined for differences in their proteins and antigens by using one- and two-dimensional electrophoretic techniques as well as immunoblotting. one-dimensional electrophoresis revealed differences in concentrations of individual bands, but not differences in the overall banding pattern. by two-dimensional electrophoretic analysis, 25 proteins were identified as strain-variable whereas the majority of protein spots wa ... | 1992 | 1576410 |
| humoral immune response to non-viable mycoplasma pulmonis in mice enhanced by dextran sulfate. | the enhancing effect of dextran sulfate on the humoral immune response to nonviable mycoplasma pulmonis in mice was evaluated by means of the indirect haemagglutination test. the serum antibody titres in mice immunized subcutaneously with a mixture of non-viable m. pulmonis and dextran sulfate were greater and persisted longer than those in mice immunized with non-viable m. pulmonis alone. deae-dextran also enhanced the humoral immune response to non-viable m. pulmonis in mice. | 1987 | 2440174 |
| effect of spontaneous pathology and thrombin on leukocyte adhesion to rat aortic endothelium. | leukocyte adhesion and other injury parameters have been studied in the aortic endothelium of sprague-dawley rats in two situations: (1) spontaneous pathology in conventional rats with antibodies to mycoplasma pulmonis and/or kilham or sendai viruses, and (2) intravascular coagulation by thrombin administration in spf rats. adhesion (esterase (+) leukocytes/mm2) in spf rats was 8 +/- 5 (n = 12). adhesion in 38% of the conventional rats was 54 +/- 27 (n = 8), half of them being non-analyzed and t ... | 1992 | 1590826 |
| detection of mycoplasma pulmonis in arthritic joints of rats by indirect immunoperoxidase staining. | neonatal and 8-week-old rats were inoculated with mycoplasma pulmonis. a portion of the animals developed polyarthritis. indirect immunoperoxidase staining was used to identify the localization of m. pulmonis within arthritic joints. m. pulmonis antigen was most often observed within cartilage in the neonatal group and in synovial tissue in the 8-week-old group. | 1989 | 2466796 |
| upper respiratory tract is the major site of antibody production in mycoplasmal induced chronic respiratory disease. | chronic respiratory disease in rats, resulting from mycoplasma pulmonis infection, provides a unique opportunity to study the interactions between systemic and mucosal immune systems in a naturally occurring infection of the respiratory tract. in the present studies, an elispot assay specific for the determination of numbers of anti-m, pulmonis antibody forming cells (afc) was developed. using this assay, the numbers of igg, igm, and iga afc in respiratory and nonrespiratory associated lymphoid ... | 1989 | 2485687 |
| short-term exposure to nitrogen dioxide enhances susceptibility to murine respiratory mycoplasmosis and decreases intrapulmonary killing of mycoplasma pulmonis. | in c57bl/6n and c3h/hen mice known to be free of all murine pathogens and matched for age, sex, microbiologic, and environmental factors, exposure to no2 for 4 h prior to exposure to infectious aerosols of mycoplasma pulmonis resulted in potentiation of murine respiratory mycoplasmosis (mrm). in the c57bl/6n mice, no2 increased the incidence of death, incidence of gross lung lesions, and incidence of microscopic lung lesions, but did not increase the incidence of infection in the lungs. nitrogen ... | 1989 | 2504091 |
| differential detection of mycoplasma pulmonis and mycoplasma arthritidis with species-specific dna probes. | mycoplasma pulmonis and mycoplasma arthritidis are both significant causes of infection in colonies of rodents, which are common experimental animals for biomedical research. since differential diagnosis has proven difficult due to similar homology between these two murine mycoplasma species, the development of a reliable identification system is of importance. in this study dna probes specific for m. pulmonis and m. arthritidis were generated after cross-hybridization between two reference stra ... | 1992 | 1643818 |
| secretory immune responses to mycoplasma pulmonis. | formalinized mycoplasma pulmonis, along with aluminum hydroxide as an adjuvant, was used to subcutaneously immunize rats in the vicinity of the salivary gland to examine the characteristics of the secretory immune response to this pathogen. the induction of specific antibody to this microorganism was detected in serum and the exocrine fluids, namely, saliva and lung lavage fluid. both immunoglobulin g (igg) and iga isotype antibodies were detected in each of these fluids after primary and second ... | 1992 | 1730465 |
| mycoplasma pulmonis possesses a novel chemoattractant for b lymphocytes. | mycoplasma pulmonis causes chronic murine respiratory mycoplasmosis, which is characterized by extensive peribronchial and perivascular infiltration of mononuclear cells, including b lymphocytes. b-lymphocyte recruitment into sites of inflammation is presently poorly understood but must involve directed chemotaxis of these cells in response to some external recruitment stimulus. in these studies, picogram amounts of m. pulmonis membrane protein were found to possess potent chemoattractant activi ... | 1992 | 1730502 |
| decreased intrapulmonary killing of mycoplasma pulmonis after short-term exposure to no2 is associated with damaged alveolar macrophages. | previous studies have shown that exposure of pathogen-free c57bl/6n mice to 5 or 10 ppm no2 increases the severity of murine respiratory mycoplasmosis and that this effect is associated with decreased intrapulmonary killing of mycoplasma pulmonis. the purposes of the present studies were to determine the effects of doses of no2 lower than 5 ppm on pulmonary clearance and to provide experimental links between no2 exposure, defects in intrapulmonary killing, and alterations in alveolar macrophages ... | 1992 | 1736750 |
| prolonged increase of corticosterone secretion by chronic social stress does not necessarily impair immune functions. | the influence of a chronic social stress upon immunity was investigated in wistar rats, submitted for four weeks to two different behavioral situations, balanced in a factorial design: housing with three females and membership rotation. the combination of these two factor led to adrenal enlargement (43.3%), thymus involution (39.5%) and increased basal corticosterone levels, all indices of activation of the hypothalamic-hypophysis-adrenal axis. however, neither natural killer cell activity, sple ... | 1992 | 1738299 |
| murine respiratory mycoplasmosis: a model to study effects of oxidants. | previous studies have shown that exposure to nitrogen dioxide at concentrations of 5 and 10 parts per million (ppm) decreases intrapulmonary killing of mycoplasma pulmonis, and that this decrease is related to increased lung lesions and mortality. the specific objectives of the present study were to titrate the effects of nitrogen dioxide on pulmonary clearance of m. pulmonis, determine the mechanisms by which this organism is killed within the lungs, and determine the target that the nitrogen d ... | 1991 | 1838926 |
| cytopathic effects of mycoplasma pulmonis in vivo and in vitro. | this study was performed to evaluate the cytopathic features resulting from mycoplasma pulmonis infection of tracheal organ cultures compared to with those seen in in vivo infection and to use this system to determine possible differences in cytopathic effects in two m. pulmonis variants found to cause different diseases in vivo. the attachment of m. pulmonis to respiratory epithelium was similar in vivo and in vitro. cytopathic effects seen in both systems were also similar in loss of tight jun ... | 1991 | 1937777 |
| vaccination of lewis rats with temperature-sensitive mutants of mycoplasma pulmonis: adoptive transfer of immunity by spleen cells but not by sera. | temperature-sensitive mutant vaccines protect rats against mycoplasma pulmonis infection. the role of the humoral or cellular immune response in resistance to mycoplasma infection was investigated by adoptive-transfer experiments. spleen cells from lewis rats vaccinated but not challenged with wild-type organisms (vaccinated) and spleen cells from rats vaccinated (or not) and challenged were effective in preventing syngeneic recipients from developing respiratory disease. there was also a signif ... | 1991 | 1987049 |
| a phylogenetic analysis of the mycoplasmas: basis for their classification. | small-subunit rrna sequences were determined for almost 50 species of mycoplasmas and their walled relatives, providing the basis for a phylogenetic systematic analysis of these organisms. five groups of mycoplasmas per se were recognized (provisional names are given): the hominis group (which included species such as mycoplasma hominis, mycoplasma lipophilum, mycoplasma pulmonis, and mycoplasma neurolyticum), the pneumoniae group (which included species such as mycoplasma pneumoniae and mycopla ... | 1989 | 2592342 |
| mycoplasma pulmonis infections cause long-lasting potentiation of neurogenic inflammation in the respiratory tract of the rat. | these experiments were done to learn whether mycoplasma pulmonis infections of the respiratory tract of rats can potentiate "neurogenic inflammation" and whether this potentiation is amplified by factors that exacerbate the infections. pathogen-free f344 rats were inoculated intranasally with m. pulmonis or with sterile culture medium and then lived for 4 wk in an ammonia-free atmosphere or in air containing ammonia (100 parts per million). neurogenic inflammation was evoked by an intravenous in ... | 1991 | 1999495 |
| sensitive detection of mycoplasma pulmonis by using the polymerase chain reaction. | detection of mycoplasma pulmonis was examined by using the polymerase chain reaction (pcr) for amplifying a specific dna sequence. in gel electrophoresis which was conducted to detect the amplified products, only 1 pg of m. pulmonis dna could be detected following 30 cycles of amplification, while no amplified product was detected even from 1 microgram of m. arthritidis or m. neurolyticum dna. furthermore, 10 colony-forming units of m. pulmonis could be detected by direct amplification from the ... | 1990 | 2105895 |
| alterations in lung prostaglandin synthesis and release in murine respiratory mycoplasmosis. | pathogenetic mechanisms in murine respiratory mycoplasmosis are poorly understood; however, non-specific immune responses appear to be important in controlling the growth of mycoplasma pulmonis in vitro. to date, no study has examined the role of pulmonary prostaglandin production during the development of m. pulmonis infection. the present study was designed to determine if alterations in pulmonary prostaglandin synthesis and release occur in m. pulmonis infection and the possible role for pros ... | 1990 | 2140452 |
| resistance to mycoplasma pulmonis mediated by activated natural killer cells. | infection of c57bl/6j mice with mycoplasma pulmonis (mp) enhanced nk cell function 3-7 days later, as detected by in vitro and in vivo assays. moreover, spleen and lung cells of acutely infected c57bl/6j mice inhibited mp growth in vitro. the effectors were eliminated by treatment with anti-nk antibody in vivo and anti-asialo gm1 serum or anti-3a4 antibody plus complement in vitro. clearance of viable and radiolabeled mp from the lungs was also enhanced in acutely infected mice. acutely infected ... | 1990 | 2140583 |
| [modification of the teratogenesis of actinomycin d and cyclophosphamide by a mycoplasma pulmonis infection in rats]. | in the present investigation experiments have been made on the modification of the teratogenesis by a mycoplasma pulmonis infection in rats. substances with known teratogenic effects (actinomycin d, cyclophosphamide) were used. groups of artificially infected pregnant rats were compared with pregnant rats from the spf unit. the present study has shown that in the group of artificially infected animals no significant increase of the teratogenic effects by actinomycin was found. in the cyclophosph ... | 1990 | 2150578 |
| genetic exchange of transposon and integrative plasmid markers in mycoplasma pulmonis. | matings of genetically marked derivatives of mycoplasma pulmonis resulted in the exchange of chromosomal dna and the appearance of doubly marked transconjugants. transposons tn916 and tn4001, and a series of integrative plasmids derived from their cloned antibiotic resistance genes, were used to construct antibiotic-resistant mycoplasmal derivatives to examine this phenomenon at the molecular level. genetic exchange occurred on agar surfaces at frequencies ranging from 3.3 x 10(-4) to 6.4 x 10(- ... | 1990 | 2158966 |
| high-frequency variation in mycoplasma pulmonis colony size. | heterogeneity in colony size of the murine pathogen mycoplasma pulmonis was examined. subcloning experiments showed that colony size variation resulted from high-frequency genetic changes. about 3% of the colonies from any given subclone were variants, with as much as a fourfold change in colony diameter. when the variants were propagated in liquid broth, their doubling times in logarithmic growth phase reflected the colony sizes obtained on agar. colony size variation correlated with changes in ... | 1989 | 2768200 |
| development of a cloning system in mycoplasma pulmonis. | a system suitable for recombinant dna manipulation in mycoplasmas was developed using the cloned antibiotic-resistance genes of tn4001 and tn916. an integrative plasmid containing one of the resistance markers was inserted into the genome of mycoplasma pulmonis to form a recipient strain. this was accomplished by transformation and homologous recombination between chromosomal dna sequences cloned onto the integrative plasmid. a second vector, the cloning vector, containing the same plasmid repli ... | 1990 | 2227427 |
| genomic dna analysis of rodent mycoplasmas. | genomic dna was compared between three typical species of rodent mycoplasmas, mycoplasma pulmonis, m. arthritidis and m. neurolyticum, and between strains of these species. each of the three species showed a distinct restriction endonuclease cleavage pattern of genomic dna. the genetic heterogeneity of these species was revealed by total dna hybridization as well. in addition, the restriction endonuclease cleavage pattern of genomic dna was almost identical in three strains of m. pulmonis and in ... | 1989 | 2792203 |
| comparison of mononuclear cell subpopulations in bronchoalveolar lavage fluid in acute rejection after lung transplantation and mycoplasma infection in rats. | acute lung rejection after orthotopic left lung transplantation and mycoplasma pulmonis infection were studied immunohistologically by bronchoalveolar lavage (bal) in inbred rats using monoclonal antibodies differentiating lymphocyte and macrophage subpopulations. twenty transplants in a major histocompatibility complex (mhc)-different strain combination (brown-norway/lewis) were examined 2, 4, and 6 days after transplantation. thirty isotransplants (lewis/lewis) and normal lewis rats were used ... | 1990 | 2231090 |
| common sulfoglycolipid receptor for mycoplasmas involved in animal and human infertility. | sulfoglycolipids are ubiquitous components of the male germ cell membrane. sulfogalactoglycerolipid (sgg) is restricted to mammalian cells and has recently been implicated in sperm/egg interactions. mycoplasma infections have been implicated in infertility in a variety of species, including humans. four such species-specific mycoplasmas, ureaplasma urealyticum and mycoplasma hominis (humans), mycoplasma pulmonis (rodents), and ureaplasma diversum (cattle) are not shown to specifically recognize ... | 1990 | 2289018 |
| male germ cell specific sulfogalactoglycerolipid is recognized and degraded by mycoplasmas associated with male infertility. | sulfogalactosylglycerolipid (sgg) is the major mammalian male germ cell glycolipid and has been implicated in sperm/egg binding. mycoplasma pulmonis, a species of mollicutes, is associated with male infertility in rodents. purified sgg incubated in the presence of m. pulmonis was enzymatically degraded by both desulfation and deacylation. desulfation occurred primarily at alkaline ph, and deacylation also increased with increased ph, indicating that these represent novel enzymatic activities. di ... | 1990 | 2298820 |
| the polymerase chain reaction for mycoplasma pulmonis. | in vitro dna amplification by polymerase chain reaction was examined to detect mycoplasma pulmonis. a pair of synthetic oligonucleotide primers was constructed, and used to amplify a unique sequence of m. pulmonis dna. amplified products were detected by agarose gel electrophoresis and verified by blot hybridization with a synthetic oligonucleotide probe. this system detected cellular dna of m. pulmonis but not m. arthritidis or m. neurolyticum, and thus appears to be useful for m. pulmonis diag ... | 1990 | 2362564 |
| biological evaluation of mycoplasma pulmonis temperature-sensitive mutants for use as possible rodent vaccines. | temperature-sensitive mutants (tsms) of mycoplasma pulmonis were produced by treating the wild-type strain with n-methyl-n'-nitro-n-nitrosoguanidine. three tsms were selected at 38 degrees c, as a restrictive temperature, and at 34 degrees c, as a permissive temperature. two tsms, utcmi and utcmii, were proven to be nonpathogenic but immunogenic. in addition, they did not induce pneumonia, tracheitis, or tympanitis but did induce mild rhinitis. they were stable after 10 passages in vitro and in ... | 1990 | 2365461 |
| methodological approaches for the study of the aortic endothelium of the rat. | this report describes a new method for obtaining whole mount preparations of rat thoracic aorta which allows the study en face of the complete surface of the endothelium. a comparative study of the different techniques utilized for silver staining of endothelium has been performed. included is the description of an apparatus for perfusion fixation of rat aorta which allows sequential perfusion of several fluids at a constant pressure. conditions for perfusion fixation at physiological pressure a ... | 1987 | 2440456 |
| group g streptococcal lymphadenitis in rats. | group g streptococci which have been isolated from the oral flora of rats are also normal inhabitants of the human skin, oropharynx, gastrointestinal tract, and female genital tract. this group of streptococci can cause a wide variety of clinical diseases in humans, including septicemia, pharyngitis, endocarditis, pneumonia, and meningitis. ten days after oral gavage with 7,12-dimethylbenz[a]anthracene, 12 of 22 two-month-old, female, outbred, viral-antibody-free rats presented with red ocular a ... | 1991 | 1757539 |
| pneumocystis carinii pneumonia in the rat model. | groups of barrier-raised but not certified virus-free sprague-dawley rats, obtained from the same source over the course of several years, were placed on an identical immunosuppressive regimen. this caused reactivation of latent pneumocystis carinii infection, manifest as p. carinii pneumonia (pcp) of varying severity. rats were euthanized after 9-12 wk of immunosuppression. an assessment of the severity of the induced pcp was made, based on the total number of organisms extracted from the lungs ... | 1991 | 1818139 |
| potential subunit vaccine against mycoplasma pulmonis purified by a protective monoclonal antibody. | monoclonal antibodies (mabs) were produced against mycoplasma pulmonis (mp); some were highly protective in the treatment of experimental infections of balb/c mice. the mabs inhibited mp growth in vitro and prevented the attachment of mp to fibroblasts or to red blood cells. three separate mabs recognized 54-76% of 54 clinical isolates of mp, and the three together detected all 54 isolates. we used the mabs to purify the antigens by affinity column chromatography. the purified antigen used to va ... | 1991 | 1828319 |
| neutral endopeptidase and neurogenic inflammation in rats with respiratory infections. | neuropeptides such as substance p are implicated in inflammation mediated by sensory nerves (neurogenic inflammation), but the roles in disease of these peptides and the peptidases that degrade them are not understood. it is well established that inflammation is a prominent feature of several airway diseases, including viral infections, asthma, bronchitis, and cystic fibrosis. these diseases are characterized by cough, airway edema, and abnormal secretory and bronchoconstrictor responses, all of ... | 1989 | 2545662 |
| changes in epithelial secretory cells and potentiation of neurogenic inflammation in the trachea of rats with respiratory tract infections. | in rats respiratory tract infections due to sendai virus and coronavirus usually are transient, but they can have long-lasting consequences when accompanied by mycoplasma pulmonis infections. morphological alterations in the tracheal epithelium and a potentiation of the inflammatory response evoked by sensory nerve stimulation ("neurogenic inflammation") are evident nine weeks after the infections begin, but the extent to which these changes are present at earlier times is not known. in the pres ... | 1989 | 2552865 |
| stimulated intraphagosomal release of eosinophil peroxidase and acid phosphatase in the rats spontaneously infected with mycoplasma pulmonis: a cytochemical study. | in this study we performed a cytochemical comparison of peroxidase and acid phosphatase activities in peritoneal eosinophils from specific pathogen-free (spf) and mycoplasma pulmonis-infected rats. when eosinophils ingested polystyrene particles for 120 min, peroxidase- and acid phosphatase-positive specific granules, as well as small granules, fused to phagosomes. unusual peroxidase activity was detected in some particle-containing phagosomes in 6.8% of eosinophils from control spf rats and 31% ... | 1989 | 2599062 |
| dyspnea in aging rats due to disseminated intravascular coagulation (dic). | during an 18-month oncogenicity study using rats, approximately 10% of the animals developed a form of respiratory distress very similar to that seen in the terminal stages of chronic respiratory disease, commonly associated with mycoplasma pulmonis infection. investigation of the lungs of the affected rats revealed not only that they did not have the consolidation usually associated with chronic respiratory disease, but they also appeared macroscopically normal. further investigation of a numbe ... | 1989 | 2603331 |
| a serological survey on 15 murine pathogens in mice and rats. | a serological survey for 15 murine pathogens was performed on 269 mouse sera collected from 21 conventional and 12 barrier colonies, and on 376 rat sera collected from 21 conventional and 23 barrier colonies. animals having an antibody against at least one of the antigens were contained in 81.0% of conventional and 16.7% of barrier mouse colonies and also in 81.0% of conventional and 43.5% of barrier rat colonies. main contaminants were mouse hepatitis virus and sendai virus in mice, and sendai ... | 1986 | 3026818 |
| mycoplasma pulmonis v-1 surface protein variation: occurrence in vivo and association with lung lesions. | the v-1 antigen of mycoplasma pulmonis is exposed to the surface of the mycoplasma and has an immunoblot banding pattern that varies in vitro between and within strains. to determine if v-1 variation occurs in vivo, we infected c3h/hencr mice intranasally with 5 x 10(8) colony-forming units of m. pulmonis strain 5782c. we isolated m. pulmonis clones from the respiratory tracts of mice up to 28 days post-infection, then used anti-v-1 monoclonal antibody p39 to visualize their v-1 immunoblot bandi ... | 1989 | 2622331 |