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characterization of the gene for an immunodominant 72 kda lipoprotein of mycoplasma mycoides subsp. mycoides small colony type.with the aim of characterizing specific immunogenic proteins of mycoplasma mycoides subsp. mycoides small colony (sc) type, the aetiological agent of contagious bovine pleuropneumonia, a gene encoding a major immunogenic protein of 72 kda named p72 was cloned and expressed in escherichia coli. the expressed protein was of the same apparent molecular mass as that produced by the parent strain. the predicted molecular mass of p72, based on the dna-deduced amino acid sequence, was 61.118 kda, signi ...19969004514
cell surface antigens of mycoplasma species bovine group 7 bind to and activate plasminogen.mycoplasma species bovine group 7 bound plasminogen at the cell surface in a lysine-dependent manner. cell-bound plasminogen was rapidly activated to plasmin by exogenous urokinase, and this activity was associated with plasminogen binding capacity. binding assays using plasminogen modified with a trifunctional cross-linking agent revealed several binding proteins.200312874368
real-time polymerase chain reaction assays for the detection of members of the mycoplasma mycoides cluster.to develop real-time pcr assays for the detection and differentiation of members of the mycoplasma mycoides cluster.200818322559
mycoplasma leachii sp. nov. as a new species designation for mycoplasma sp. bovine group 7 of leach, and reclassification of mycoplasma mycoides subsp. mycoides lc as a serovar of mycoplasma mycoides subsp. capri.the mycoplasma mycoides cluster consists of six pathogenic mycoplasmas causing disease in ruminants, which share many genotypic and phenotypic traits. the m. mycoides cluster comprises five recognized taxa: mycoplasma mycoides subsp. mycoides small colony (mmmsc), m. mycoides subsp. mycoides large colony (mmmlc), m. mycoides subsp. capri (mmc), mycoplasma capricolum subsp. capricolum (mcc) and m. capricolum subsp. capripneumoniae (mccp). the group of strains known as mycoplasma sp. bovine group ...200919502315
identification and distribution of genetic markers in three closely related taxa of the mycoplasma mycoides cluster: refining the relative position and boundaries of the mycoplasma sp. bovine group 7 taxon (mycoplasma leachii).mycoplasmas belonging to the mycoplasma mycoides phylogenetic cluster are all important ruminant pathogens that are genetically closely related but differ in terms of severity and prevalence of the associated diseases. they are distributed among six taxa, the description of which has recently been amended. in the present study, dna fragments that diverge between the type strains of three taxa were enriched using suppression subtractive hybridization. of the three taxa, two were representative of ...200919696102
Mycoplasma leachii sp. nov. in Calves, China. 201121888824
phylogeny of the mycoplasma mycoides cluster based on analysis of five conserved protein-coding sequences and possible implications for the taxonomy of the group.a phylogenetic tree of the mycoplasma mycoides cluster was inferred from a set of concatenated sequences from five housekeeping genes (fusa, glpq, gyrb, lepa and rpob). the relevance of this phylogeny was reinforced by detailed analysis of the congruence of the phylogenies derived from each of the five individual gene sequences. two subclusters were distinguished. the m. mycoides subcluster comprised m. mycoides subsp. mycoides biotypes small colony (sc) and large colony (lc) and m. mycoides sub ...200717911291
characterization of a chromosomal region of mycoplasma sp. bovine group 7 strain pg50 encoding a glycerol transport locus (gtsabc).mycoplasma species bovine group 7, represented by the type strain pg50, is one of six members of the mycoplasma mycoides cluster and has been implicated in sporadic and outbreak cases of polyarthritis and mastitis in australian dairy cattle. this study describes cloning and sequencing a 7.9 kb region of the pg50 chromosome and identification of genes involved in glycerol transport (gtsa, gtsb and gtsc) that are followed by a putative lipoprotein gene lppb and a genomic locus containing two orfs ...200312576593
genetic diversity among mycoplasma species bovine group 7: clonal isolates from an outbreak of polyarthritis, mastitis, and abortion in dairy cattle.a comprehensive genetic analysis of 60 mycoplasma sp. bovine group 7 isolates from different geographic origins and epidemiological settings is presented. twenty-four isolates were recovered from the joints of calves during sporadic episodes of polyarthritis in geographically distinct regions of queensland and new south wales, australia, including two clones of the type strain pg5o. a further three australian isolates were also recovered from the tympanic bulla, retropharyngeal lymph node and th ...200111669541
mastitis, polyarthritis and abortion caused by mycoplasma species bovine group 7 in dairy cattle.to report an outbreak of mastitis, polyarthritis and abortion caused by mycoplasma sp bovine group 7 in three large, centrally-managed dairies and to review the relevant literature.200011194719
comparison of the 16s-23s rrna intergenic spacer regions among strains of the mycoplasma mycoides cluster, and reassessment of the taxonomic position of mycoplasma sp. bovine group 7.nucleotide sequence analysis of the 16s-23s rrna intergenic spacer regions of six type or reference strains belonging to the mycoplasma mycoides cluster and of mycoplasma putrefaciens suggested the presence of two subclusters. one subcluster comprised m. mycoides subsp. mycoides small colony (sc) type, m. mycoides subsp. mycoides large colony (lc) type and m. mycoides subsp. capri, whereas the second subcluster comprised mycoplasma capricolum subsp. capricolum, m. capricolum subsp. capripneumoni ...200010843078
genetic and serological analysis of lipoprotein lppa in mycoplasma mycoides subsp. mycoides lc and mycoplasma mycoides subsp. capri.the genes encoding the 62-kda lipoproteins from the mycoplasma mycoides subsp. mycoides large-colony type (lc) strain y-goat and the m. mycoides subsp. capri strain pg3 were cloned and analyzed by sequencing. these two lipoproteins have been named lppa[mmymylc] and lppa[mmyca], and their corresponding genes have been named lppa[mmymylc] and lppa[mmyca], respectively. the nucleotide and deduced amino acid sequences of these two lipoproteins showed a very high degree of similarity between these tw ...199910066658
genetic and serological analysis of the immunogenic 67-kda lipoprotein of mycoplasma sp. bovine group 7.the gene encoding a lipoprotein of 67 kda, named p67, was cloned from mycoplasma sp. bovine group 7 strain pg50 and expressed in escherichia coli k12. analysis of the amino acid sequence derived from the dna sequence of the p67 gene revealed a typical prokaryotic signal peptidase ii membrane lipoprotein lipid attachment site and a transmembrane structure domain in the leader sequence at the amino-terminal end of the protein. protein p67 showed 91% identical amino acid residues to the lipoprotein ...19989766210
development of a sensitive nested pcr method for the specific detection of mycoplasma mycoides subsp. mycoides sc.a specific and sensitive test for the detection of mycoplasma mycoides subsp. mycoides small colony type (sc), the aetiological agent of contagious bovine pleuropneumonia (cbpp) was developed using two nested pcr reactions. the pcr reactions are based on the nucleotide sequence of lipoprotein p72 of m. mycoides subsp. mycoides sc. the two specific oligonucleotide primer pairs were chosen to match those sequence segments of the p72 gene which differ most from the gene of the closely related lipop ...19979160324
mastitis in cows and polyarthritis and pneumonia in calves caused by mycoplasma species bovine group 7. 19854026723
complete genome sequences of mycoplasma leachii strain pg50t and the pathogenic mycoplasma mycoides subsp. mycoides small colony biotype strain gladysdale.mycoplasma mycoides subsp. mycoides small colony biotype (sc) is the high-consequence animal pathogen causing contagious bovine pleuropneumonia. we report the complete genome sequences of the pathogenic strain m. mycoides subsp. mycoides sc gladysdale and a close phylogenetic relative, mycoplasma leachii pg50(t), another bovine pathogen of the m. mycoides phylogenetic clade.201222843585
the origin of the 'mycoplasma mycoides cluster' coincides with domestication of ruminants.the 'mycoplasma mycoides cluster' comprises the ruminant pathogens mycoplasma mycoides subsp. mycoides the causative agent of contagious bovine pleuropneumonia (cbpp), mycoplasma capricolum subsp. capripneumoniae the agent of contagious caprine pleuropneumonia (ccpp), mycoplasma capricolum subsp. capricolum, mycoplasma leachii and mycoplasma mycoides subsp. capri. cbpp and ccpp are major livestock diseases and impact the agricultural sector especially in developing countries through reduced food ...201222558362
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