| improving the specificity and yield of the contagious bovine pleuropneumonia complement fixation test antigen. | several methods for increasing yield and specificity of the contagious bovine pleuropneumonia complement fixation test antigen which is derived from mycoplasma mycoides subsp mycoides, strain v5, were examined. changes in culture conditions that increased the cell mass per unit volume of culture did not result in comparable increase in antigen yield. sixteen to 60-day-old cultures yielded more boiled cell antigen than younger cultures. some antigen in young cultures appeared to be masked, probab ... | 1976 | 57633 |
| immunoprecipitation of triton x-100-solubilized mycoplasma mycoides proteins. | mycoplasma mycoides subsp. mycoides (pg1 and strain y) proteins were solubilized in triton x-100, and the antigenic proteins were precipitated from this complex mixture by addition of antiserum and then separated by two-dimensional gel electrophoresis. of the 300 proteins solubilized, about 10 were precipitated. proteins of pg1, a slow-growing, small colony (sc) strain, were precipitated by antiserum to pg1 and by antiserum to strain y, a fast-growing, large colony (lc) strain. similarly, strain ... | 1979 | 93614 |
| enzymes of purine metabolism in mycoplasma mycoides subsp. mycoides. | the major pathways of ribonucleotide biosynthesis in mycoplasma mycoides subsp. mycoides were proposed previously from studies of its usage of radioactive purines and pyrimidines. to interpret more fully the pattern of purine usage, we have assayed cell-free extracts of this organism for several enzymes associated with the salvage synthesis of purine nucleotides. m. mycoides possessed phosphoribosyltransferases for adenine, guanine, and hypoxanthine, purine nucleoside phosphorylase, gmp reductas ... | 1978 | 207675 |
| enzymes of pyrimidine metabolism in mycoplasma mycoides subsp. mycoides. | the major pathways of ribonucleotide biosynthesis in mycoplasma mycoides subsp. mycoides have been proposed from studies on its use of radioactive purines and pyrimidines. to interpret more fully the observed pattern of pyrimidine usage, cell extracts of this organism have been assayed for several enzymes associated with the salvage synthesis of pyrimidine nucleotides. m. mycoides possessed uracil phosphoribosyltransferase, uridine phosphorylase, uridine (cytidine) kinase, uridine 5'-monophospha ... | 1979 | 220209 |
| pathways of nucleotide biosynthesis in mycoplasma mycoides subsp. mycoides. | by measuring the specific activity of nucleotides isolated from ribonucleic acid after the incorporation of (14)c-labeled precursors under various conditions of growth, we have defined the major pathways of ribonucleotide synthesis in mycoplasma mycoides subsp. mycoides. m. mycoides did not possess pathways for the de novo synthesis of nucleotides but was capable of interconversion of nucleotides. thus, uracil provided the requirement for both pyrimidine ribonucleotides. thymine is also required ... | 1977 | 324972 |
| inhibition of the growth of some strains of mycoplasma mycoides subsp mycoides by the blood of certain horses. | when incorporated in solid medium at a concentration of 15 per cent, the defibrinated blood of certain horses strongly suppressed the growth of some, but not all, strains of mycoplasma mycoides subsp mycoides so that many colonies failed to develop to a visible size. blood from a single rabbit was tested and found to exert a similar effect. there was striking variation in the degree of inhibition produced by different samples of horse blood and, of five strains of the organism examined, the t1 v ... | 1977 | 337432 |
| subdivision of mycoplasma mycoides subsp. mycoides from cattle and goats into two types. | some strains of mycoplasma mycoides subsp. mycoides, mostly isolated from goats, grow to greater turbidity in broth and form larger colonies on solid medium than does the type strain, pg1. these strains also digest casein, liquefy inspissated serum actively and survive longer at 45 degrees c and are referred to as lc (large colony) strains. strains more closely resembling pg1 have been called sc (small colony) strains. the sc strains comprise those from contagious bovine pleuropneumonia (cbpp) a ... | 1978 | 356841 |
| relationships between strains of mycoplasma mycoides subspp. mycoides and capri studied by two-dimensional gel electrophoresis of cell proteins. | strains of mycoplasma mycoides subsp. mycoides have been divided into small colony (sc) and large colony (lc) types (cottew & yeats, 1978). the protein patterns of representative strains of these two types and of m. mycoides subsp. capri were compared by a high resolution, two-dimensional gel electrophoretic method. the results suggest that the lc strains are more closely related to m. mycoides subsp. capri than to the sc strains of m. mycoides subsp. mycoides. | 1978 | 370343 |
| immunosuppressive effect of trypanosoma congolense and trypanosoma vivax on the secondary immune response of cattle to mycoplasma mycoides subsp mycoides. | trypanosoma congolense and t vivax infections in cattle were shown to have a suppressive effect on the secondary humoral immune response to m mycoides. the trypanosome infections caused an involution of the thymus. the secondary response was biphasic in nature and was unrelated to infection with trypanosomes. | 1978 | 373035 |
| differentiation of mycoplasma mycoides subsp. mycoides from certain closely related caprine mycoplasmas by mycoplasmaemia and cross-protection tests in mice. | in recent years, mycoplasma taxonomists have found that numerous mycoplasma strains from goats are serologically indistinguishable from mycoplasma mycoides subsp. mycoides, the causative agent of contagious bovine pleuropneumonia (cbpp), by routinely used tests, e.g. the metabolism- and growth-inhibition tests. as a result, such organisms are now openly referred to as m. mycoides subsp. mycoides. seven of these so-called m. mycoides subsp. mycoides strains from goats were compared with two strai ... | 1979 | 376695 |
| artificial reproduction of arthritis in calves by intubation of a virulent strain of mycoplasma mycoides subsp mycoides. | | 1979 | 377780 |
| detection of antibody against mycoplasma mycoides subsp mycoides in cattle by an enzyme-linked immunosorbent assay. | the results of an enzyme-linked immunosorbent assay (elisa) for the detection of antibody against mycoplasma mycoides subsp mycoides are presented. antibody was detected in the sera of cattle at least 19 months after recovery from an infection and at least 23 months after vaccination. almost half the sera of some animals in an area of nigera where contagious bovine pleuropneumonia is enzootic contained antibody. antibody was rarely detected when the same sera were examined by other established s ... | 1979 | 390853 |
| virulence of established vaccine strains and artificially passaged field strain of mycoplasma mycoides subsp mycoides. | the virulence of mycoplasma mycoides for mice, which appears to parallel virulence for cattle, was assessed in respect of four vaccine strains well known for their use in the control of contagious bovine pleuropneumonia. the t1 strain was slightly more virulent than v5, and both were much more virulent than f and kh3j, which could not be distinguished from each other. there was no significant difference between cultures of the t1 strain supplied by three different vaccine-producing laboratories, ... | 1976 | 772773 |
| an anamnestic response to challenge with virulent mycoplasma mycoides subsp. mycoides of cattle immune to contagious bovine pleuropneumonia. | | 1976 | 780380 |
| studies on the origin of false positive reactions to the complement-fixation test for contagious bovine pleuropneumonia. | serums of cattle free from contagious bovine pleuropneumonia (cbpp) were tested in complement fixation (cf) tests using 3 antigens; these were the standard antigen (sa) used to test to cbpp in australia, an ethanol extract antigen (ea) also prepared from mycoplasma mycoides subsp. mycoides (m. mycoides) and an antigen prepared from a group 7 bovine mycoplasma isolated from arthritis (aa). the serums included 146 which fixed complement with sa. eighty percent of these false-positive serums reacte ... | 1976 | 791232 |
| caprine pneumonia iv: pathogenicity of mycoplasma mycoides subspecies capri and caprine strains of mycoplasma mycoides subsp. mycoides for goats. | | 1976 | 791976 |
| acute respiratory, circulatory and pathological changes in the calf after intravenous injections of the galactan from mycoplasma mycoides subsp. mycoides. | twenty of 28 calves, 10-12 weeks of age when given intravenous injections of the galactan from mycoplasma mycoides subsp. mycoides, showed transient apnoea, increased pulmonary arterial and decreased systemic arterial blood pressures, and increased packed-cell volume. necropsy revealed haemorrhages associated with alveolar ducts and vessel walls, areas of pulmonary oedema, usually associated with the haemorrhages, dilated airways and, in some, capillary thrombosis. animals that had shown changes ... | 1976 | 794475 |
| mycoplasma mycoides subspecies mycoides as the cause of a subauricular abscess and mastitis in a goat. | a 6-year-old female goat was admitted with right-sided subauricular swelling and facial nerve paralysis. mastitis developed subsequently. the subauricular swelling localized to a mass and was excised. mycoplasma mycoides subsp mycoides was cultured from samples obtained from the mass and mammary gland. after clinical improvement, the goat was discharged to the owner with instructions to isolate the goat and to submit milk samples from the rest of the herd for microbial culturing. mycoplasma spp ... | 1992 | 1429189 |
| nucleotide sequence of mycoplasma mycoides subspecies mycoides plasmid pkmk1. | to facilitate the development of mycoplasmal cloning vectors, we have determined the nucleotide sequence of pkmk1, a cryptic plasmid isolated from mycoplasma mycoides subsp. mycoides. it is 1875 bp in length and contains two open reading frames (orfs) that share homology with orfs from members of a large family of gram-positive bacterial plasmids which replicate via a single-stranded dna intermediate. putative origins of replication and candidate cloning sites have been identified. | 1992 | 1518915 |
| isolation of a second cryptic plasmid from mycoplasma mycoides subsp. mycoides. | plasmids have rarely been detected in organisms constituting the genus mycoplasma. recently, the isolation of a cryptic plasmid from mycoplasma mycoides subsp. mycoides has been described, and we report here the isolation of a second cryptic plasmid from this species. restriction map and southern blot analyses show that the second plasmid is distinct from the previously described plasmid, although a limited region of homology was detected. the availability of mycoplasmal cryptic plasmids may lea ... | 1990 | 2096401 |
| genomic maps of some strains within the mycoplasma mycoides cluster. | genomic restriction maps for the small colony (sc) strains (pg1, kh3j, gladysdale, and v5) of mycoplasma mycoides subsp. mycoides (the agent of contagious bovine pleuropneumonia) and for mycoplasma strain pg50 (classified as bovine serogroup 7), with respective sizes of 1,280, 1,280, 1,260, 1,230, and 1,040 kbp, were compared with the map (1,200 kbp) for a large colony strain (y goat) of m. mycoides subsp. mycoides. the number and order of all mapped restriction sites were fully conserved in the ... | 1990 | 2254284 |
| a mutant of mycoplasma mycoides subsp. mycoides lacking the h2o2- producing enzyme l-alpha-glycerophosphate oxidase. | cells of mycoplasma mycoides subsp. mycoides grown without stirring or aeration in batch culture, and resuspended in a salts solution, oxidised a range of carbohydrates including glycerol. the rate of glycerol oxidation was not reduced when cells were passaged more than 20 times in batch culture. however, in cells grown in stirred and aerated chemostat culture for 100 generations the ability to oxidise glycerol, but not other carbohydrates, was lost or greatly reduced. a mutant strain isolated f ... | 1990 | 2283028 |
| human ureaplasmas show diverse genome sizes by pulsed-field electrophoresis. | contour clamped homogeneous field (chef) agarose gel electrophoresis (age), ramped to give linear separation of dna molecules of 600-1600 kilobase pairs (kbp), was used to determine mobilities for full-sized genomic dna of the serotype standard strains of the human genital mollicutes, ureaplasma urealyticum relative to yeast chromosomal dna markers. indicated genome sizes (in kbp) were 760 for the four biotype 1 strains and 840-1140 for eleven biotype 2 strains. other estimates were: 720 for myc ... | 1990 | 2326188 |
| demonstration of cross-reactive antigens in f38 and related mycoplasmas by enzyme-linked immunosorbent assay (elisa) and immunoblotting. | the elisa and an immunoblotting technique were used to study f38-type mycoplasmas - an important cause of contagious caprine pleuropneumonia - and a number of related mycoplasma species, subspecies, types or serogroups. two-way elisa cross-reactivity was demonstrated between five mycoplasmas, namely strain f38, mycoplasma mycoides subsp. mycoides (lc strain), m. equigenitalium, m. primatum and bovine serogroup 7. in addition one-way cross-reactivity was demonstrated between f38 and each of the f ... | 1985 | 2410491 |
| [experimental reproduction and the course of contagious bovine pleuropneumonia in a group of cattle and goats: anatomoclinical aspects]. | five cows were inoculated with 3.10(9) cfu mycoplasma mycoides subsp. mycoides. five other cows and five goats were placed in close contact with inoculated cows. clinical observations, serological survey and microbial excretion by culture for mycoplasma from tracheobronchial washes liquid were carried out during 4 months. mycoplasmas excretion is detected before antibodies responses on all 10 infected cows. the cows never presented clinical respiratory signs and showed no or light lesions but on ... | 1989 | 2485540 |
| [correlation of the excretion of mycoplasma and kinetics of antibodies detected by complement fixation, passive hemagglutination and rapid seroagglutination in mycoplasma mycoides subsp. mycoides sc experimental infection in cattle]. | during an experimental reproduction of cbpp, 5 inoculated cows and 5 contacts cows were bled twice a week and antibodies research was performed using complement fixation test (cft), passive haemagglutination test (phat) and slide agglutination test (sat). in the same period, trachobronchial washings were performed weekly to detect and to count mycoplasma mycoides subsp. mycoides sc. for four months these tests were used to compare antibodies kinetics with kinetics of mycoplasmas excretion. with ... | 1989 | 2485541 |
| location of sites of transposon tn916 insertion in the mycoplasma mycoides genome. | the genome of mycoplasma mycoides subsp. mycoides gc1176-2 was cleaved into six large fragments by the endonuclease kpni which also cleaved the transposon tn916 once. this has allowed genomic mapping of insertion sites for 50 transformants of gc1176-2 containing tn916. almost all of the mapped sites were clearly separate. the transformants provide a bank of genomes each with a kpni site at a different position to facilitate mapping of gene loci. | 1989 | 2556378 |
| construction of the mycoplasma evolutionary tree from 5s rrna sequence data. | the 5s rrna sequences of eubacteria and mycoplasmas have been analyzed and a phylogenetic tree constructed. we determined the sequences of 5s rrna from clostridium innocuum, acholeplasma laidlawii, acholeplasma modicum, anaeroplasma bactoclasticum, anaeroplasma abactoclasticum, ureaplasma urealyticum, mycoplasma mycoides mycoides, mycoplasma pneumoniae, and mycoplasma gallisepticum. analysis of these and published sequences shows that mycoplasmas form a coherent phylogenetic group that, with c. ... | 1985 | 2579388 |
| homology of mycoplasma plasmid padb201 and staphylococcal plasmid pe194. | the complete nucleotide sequence of padb201, a 1.7-kilobase cryptic plasmid from mycoplasma mycoides subsp. mycoides, is reported. the sequence contains a single large open reading frame capable of coding for a polypeptide of up to 198 codons long. the sequence of the putative polypeptide shows significant similarity to that of the repf gene product of staphylococcal plasmid pe194. | 1989 | 2644210 |
| relationship between mycoplasma mycoides subsp. mycoides ('large-colony' strains) and m. mycoides subsp. capri, as indicated by numerical analysis of one-dimensional sds-page protein patterns. | twenty-five strains classified as mycoplasma mycoides subsp. mycoides lc or subsp. capri have been compared by one-dimensional sds-page of their cellular proteins. a computerized numerical analysis revealed that the protein patterns of all but two aberrant strains formed one large phenon that separated clearly from representatives of the four other members of the 'm. mycoides cluster' at a similarity level (s) of 66% and which remained undivided at up to 78% s. at higher similarity levels, these ... | 1989 | 2693591 |
| preparation and fige separation of infrequent restriction fragments from mycoplasma mycoides dna. | use of procedures for obtaining satisfactory preparation and digestion of intact dna of mycoplasma mycoides subsp. mycoides y in agarose blocks is reported. the use of inverted field agarose gel electrophoresis (fige) for separation of the small number of fragments derived from the genome by several restriction endonuclease digestions is shown. an effect that fragments containing replication forks remain in the well during fige, distorting the representative yield of restriction fragments on the ... | 1988 | 2833729 |
| a physical map of the genome of mycoplasma mycoides subspecies mycoides y with some functional loci. | a physical map is presented for the 1200 kb genome of mycoplasma mycoides subsp. mycoides y, locating 32 cleavage sites for 8 restriction endonucleases. the large restriction fragments involved were separated and sized by pulsed-field agarose gel electrophoresis. their locations on the map were determined by probing southern blots of digests with individual fragments isolated from other digests and by correlating the products of double and triple digestions. loci for 2 ribosomal rna operons and ... | 1988 | 2840640 |
| isolation and restriction endonuclease analysis of a mycoplasma plasmid. | a 1.7-kb plasmid was isolated from a large colony type strain of mycoplasma mycoides subsp. mycoides. it was cloned into the escherichia coli vector m13mp19, and dna from the resultant recombinant was used to construct a restriction map of the plasmid. because mycoplasmas show deviation from normal coding patterns, the availability of a mycoplasma plasmid could be useful in the development of a cloning vector for them. | 1988 | 2840682 |
| isolation and characterization of mycoplasma mycoides subsp. mycoides mutants deficient in nucleoside monophosphate transport. | to select mutants lacking damp uptake, log-phase cells of mycoplasma mycoides subsp. mycoides y were incubated with high-specific-activity [32p]damp and then stored several weeks at -20 degrees c to allow 32p decay before plating out. mutants were screened for lack of labeling by [32p]damp. two mutants were studied further by uptake and growth experiments with other nucleotides. | 1988 | 3056924 |
| pathogenicity of mycoplasma mycoides subsp mycoides (large-colony type) for sheep udder. | | 1988 | 3063247 |
| the occurrence of infectious diseases in mixed farming of domesticated wild herbivores and livestock in kenya. ii. bacterial diseases. | the prevalence of antibodies to brucella spp., mycobacterium paratuberculosis and the mycoplasma spp. causing contagious bovine pleuropneumonia and contagious caprine pleuropneumonia was determined in various species of ruminants on a ranch in the semi-arid zone of southeastern kenya. antibody titers to brucella spp. were found in eland (taurotragus oryx), oryx (oryx beisa) and camels (camelus dromedarius). reactors were not found in buffalo (syncerus caffer), sheep (ovis aries) and goats (capra ... | 1988 | 3373635 |
| comparison of caprine mycoplasmosis caused by mycoplasma capricolum, mycoplasma mycoides subsp. mycoides, and mycoplasma putrefaciens. | we reviewed the disease process in goats caused by mycoplasma capricolum, m. mycoides subsp. mycoides, and m. putrefaciens and compared mycoplasma-infected goats (particularly young kids) for microanatomic changes. m. capricolum and m. mycoides subsp. mycoides cause a nearly identical disease: septicemia leading to pyrexia, high morbidity and mortality, interstitial pneumonia, arthritis (including polyarthritis), and mastitis. until recently, m. putrefaciens was known only as a cause of mastitis ... | 1987 | 3667229 |
| observations on experimental inactivated vaccines for contagious bovine pleuropneumonia. | in two trials the efficacy of inactivated vaccines against contagious bovine pleuropneumonia was tested by exposing vaccinated cattle to droplet infection provided by close contact with experimentally infected 'donors'. complete protection was given by an extreme form of vaccination in which a heavy suspension of killed mycoplasma mycoides subsp. mycoides emulsified with freund's complete adjuvant was given in two large doses. 'mouse-protective antibody' (mpa) was also produced, i.e. serum trans ... | 1986 | 3782785 |
| mycoplasma mycoides subsp mycoides in the ears of goats associated with an outbreak of systemic mycoplasmosis. | | 1985 | 3833199 |
| studies on mycoplasma mycoides subsp. mycoides (lc) in lambs and calves. | six cesarean-derived lambs were inoculated either with 4.5 x 10(4), 4.5 x 10(6) or 4.5 x 10(8) mycoplasma mycoides subsp. mycoides intratracheally. one animal receiving the intermediate dose died four days post-inoculation, the two receiving the high dose died six days postinoculation, while one receiving the low dose died eight days postinoculation. the two surviving lambs were challenged on day 20 postinoculation with 1 x 10(8) organisms subcutaneously and 2 x 10(9) organisms intravenously. on ... | 1985 | 3893663 |
| kinetics of utilization of organic substrates by mycoplasma mycoides subsp. mycoides in a salts solution: a flow-microcalorimetric study. | the metabolism of various organic substrates by suspensions of mycoplasma mycoides subsp. mycoides in a salts solution was followed by microcalorimetry. enthalpy changes associated with metabolism were in good agreement with theoretical values. substrate utilization showed michaelis kinetics, allowing saturation constants (km) and maximum specific rates of substrate utilization (vmax) to be determined. in cells grown on a complex medium containing glucose, km values were: glucose, fructose, n-ac ... | 1985 | 3903037 |
| enzymes of intermediary carbohydrate metabolism in ureaplasma urealyticum and mycoplasma mycoides subsp. mycoides. | cell extracts of ureaplasma urealyticum and mycoplasma mycoides were examined for enzymes of intermediary carbohydrate metabolism using a sensitive radiochemical assay procedure. for m. mycoides, the enzyme activities detected were supporting evidence for the existence of a glycolytic pathway giving lactate anaerobically and acetate aerobically. u. urealyticum also had activities of many glycolytic enzymes. enzymes of the pentose phosphate pathway occurred in both m. mycoides and u. urealyticum. ... | 1985 | 3906033 |
| the identification of an organism isolated from maned sheep as mycoplasma mycoides subsp. mycoides. | | 1972 | 4569041 |
| enzymes of pyrimidine deoxyribonucleotide metabolism in mycoplasma mycoides subsp. mycoides. | cell-free extracts of mycoplasma mycoides subsp. mycoides were assayed for enzymes associated with the salvage synthesis of pyrimidine deoxyribonucleotides. they possessed kinases for deoxycytidine, (d)cmp, thymidine (deoxyuridine), dtmp, and nucleoside diphosphates; dctpase and dutpase; dcmp deaminase; thymidine (deoxyuridine) phosphorylase; and dump (dtmp) phosphatase. the existence of these enzymic activities together with ribonucleoside diphosphate reductase explains the capacity of cytidine ... | 1983 | 6139361 |
| formylation of methionyl-transfer ribonucleic acid in mycoplasma mycoides subsp. mycoides. | mycoplasma mycoides subsp. mycoides grows readily but does not formylate methionyl-transfer ribonucleic acid in a defined medium without an added formyl donor. formylation occurs when the medium is supplemented with n5,n10-methenyltetrahydrofolate or n10-formyltetrahydrofolate, but not with folate. | 1981 | 6163766 |
| purine metabolism in acholeplasma laidlawii b: novel ppi-dependent nucleoside kinase activity. | acholeplasma laidlawii b-pg9 was examined for 16 cytoplasmic enzymes with activity for purine salvage and interconversion. phosphoribosyltransferase activities for adenine, guanine, xanthine, and hypoxanthine were shown. adenine, guanine, xanthine, and hypoxanthine were ribosylated to their nucleoside. adenosine, inosine, xanthosine, and guanosine were converted to their base. no atp-dependent phosphorylation of nucleosides to mononucleotides was found. however, ppi-dependent phosphorylation of ... | 1984 | 6330034 |
| caprine mycoplasmosis: widespread infection in goats with mycoplasma mycoides subsp mycoides (large-colony type). | a mycoplasma survey of goats with a history of mastitis, polyarthritis, and pneumonia revealed a high incidence of mycoplasma mycoides subsp mycoides (large-colony type). during the early winter kidding season of 1979-1980, kid morbidity in a large commercial dairy herd, evidenced by hyperthermia, polyarthritis, and pneumonia, exceeded 90%. in excess of 200 kids died. colostral cultures of the parent does yielded 157 isolations from 605 goats (26%). additional isolations were made from goats wit ... | 1983 | 6338774 |
| pathways of pyrimidine deoxyribonucleotide biosynthesis in mycoplasma mycoides subsp. mycoides. | by measuring the specific activity of deoxyribonucleotides isolated from dna after the incorporation of 14c-labeled precursors with and without competition from other nucleotide precursors, we defined the major pathways of pyrimidine deoxyribonucleotide synthesis in mycoplasma mycoides subsp. mycoides. uracil, guanine, and thymine are required for the synthesis of nucleotides. cytidine competed effectively with uracil to provide all of the deoxycytidine nucleotide, as well as most of the deoxyri ... | 1983 | 6339469 |
| milkborne outbreak of mycoplasma mycoides subspecies mycoides infection in a commercial goat dairy. | a commercial goat dairy comprised of about 300 lactating does suddenly began to have a problem with polyarthritis in the kids and clinical mastitis in the does. the problem began 3 to 4 weeks following the introduction of 27 lactating does from a herd with a history of mastitis caused by mycoplasma mycoides subsp mycoides (mmm). over 2 kidding seasons (about 12 months), approximately 170 kids and 70 does died or were euthanatized. affected kids were 2 to 8 weeks old, had multiple warm swollen jo ... | 1983 | 6348004 |
| contagious bovine pleuropneumonia produced by aerosols artificially generated from cultures of mycoplasma mycoides subsp. mycoides. | | 1983 | 6349746 |
| growth characteristics of large- and small-colony types of mycoplasma mycoides subsp. mycoides on 5% sheep blood agar. | mycoplasma mycoides subsp. mycoides of the large-colony (lc) type was isolated in pure culture on 5% sheep blood agar plates inoculated with lung specimens from a 4-month-old toggenburg goat. the growth characteristics of this isolate, of four known lc types, and of five known small-colony (sc) types of m. mycoides subsp. mycoides were compared on 5% sheep blood agar at 2, 5, and 7 days. the sc types were not visible at 2 days and did not grow larger than 0.1 mm, whereas the lc types were visibl ... | 1983 | 6355169 |
| susceptibility of goats and calves after experimental inoculation or contact exposure to a canadian strain of mycoplasma mycoides subsp. mycoides isolated from a goat. | transmissibility of mycoplasma mycoides subsp. mycoides infection from experimentally inoculated goats to other goats and calves was studied. eight goats and six calves were housed in an 18 m2 room. six of the goats were inoculated endobronchially with strain d44 isolated from a natural case of polyarthritis in ontario. these six goats died within a week of mycoplasma septicemia. the two contact goats or the six calves never showed signs of disease and m. mycoides subsp. mycoides was not recover ... | 1983 | 6365296 |
| uptake and utilization of deoxynucleoside 5'-monophosphates by mycoplasma mycoides subsp. mycoides. | mycoplasma mycoides subsp. mycoides has been shown to possess an unusual capacity for the uptake and utilization of exogenous deoxyribonucleoside 5'-monophosphates intact without prior dephosphorylation. in this study, it was found that once inside the cell, deoxyribonucleoside 5'-monophosphates were rapidly phosphorylated to the triphosphate level and incorporated into dna. catabolism of deoxyribonucleoside 5'-monophosphates was also observed. competition studies indicated that a single uptake ... | 1984 | 6373743 |
| electrophoretic analysis of proteins from mycoplasma capricolum and related serotypes using extracts from intact cells and from minicells containing cloned mycoplasma dna. | the acidic proteins of six different mycoplasma serotypes causing bovine or caprine pleuropneumonia were compared by two-dimensional gel electrophoresis of extracts of 35s-labelled cells. the organisms investigated were mycoplasma mycoides subsp. mycoides (pg1), m. mycoides subsp. mycoides (y-goat), m. mycoides subsp. capri (pg3), m. capricolum (california kid), the unclassified bovine serogroup 7 of leach (pg50) and the f38-like group (f38). the results suggested a close relationship between m. ... | 1984 | 6384426 |
| an analysis of mycoplasma mycoides by in vivo methods. | fresh isolates of the small colony (sc) type of mycoplasma mycoides subsp. mycoides readily produced mycoplasmaemia in mice inoculated i.p. the mycoplasmaemic capability of large colony (lc) strains was much less by comparison. cross-immunization and -hyperimmunization tests in mice were made by injecting vaccines i.v., challenging i.p., and assessing the results on the presence or absence of mycoplasmaemia at daily intervals thereafter, as demonstrated by tail-blood culture in a selective mediu ... | 1984 | 6392180 |
| mycoplasma mycoides infections in ruminants: the current situation. | recent reports of naturally occurring infections with the large colony variant of mycoplasma mycoides subsp. mycoides are collated and considered together with the results of experimental pathogenicity studies. the information is assessed to indicate where further investigation would add to knowledge of m. mycoides subsp. mycoides as a ruminant pathogen. | 1984 | 6392187 |
| effect of the caprine variant of mycoplasma mycoides subsp mycoides on endothelium, monocytes, and complement of guinea pig, calf, sheep, and goat serum. | the caprine variant of mycoplasma mycoides subsp mycoides causes septicemia with coagulopathy in goats. pathogenetic mechanisms that might explain the coagulopathy, the ability of the mycoplasma to persist in the blood, and its specificity for goats were studied. severe endothelial damage was seen by electron microscopy of goat aorta tissue exposed in vitro to 10(7) colony-forming units of mycoplasmas. the mycoplasma did not damage 51cr-labeled adherent cells from peripheral blood of goats. the ... | 1984 | 6395728 |
| immunization against mycoplasma pneumoniae disease: a review. | there have been three basic approaches to vaccine development: inactivated, live attenuated, and purified cell component vaccines. with rare exception, inactivated vaccines have proved disappointing. because men and animals recovering from naturally occurring diseases are resistant to rechallenge, the effectiveness of live vaccines was thought promising. however, the only live mycoplasma vaccine effective under field trial conditions is that prepared from mycoplasma mycoides subsp. mycoides for ... | 1984 | 6439678 |
| pathogenetic mechanisms of mycoplasma mycoides subsp. mycoides septicemia in goats. | the variant best known as the large-colony type of m. mycoides subsp. mycoides is responsible for severe outbreaks of septicemia with coagulopathy in goats. our objective was to study aspects of the pathogenesis that might explain the coagulopathy, the persistence of mycoplasmas in the blood of septicemic goats, and the host specificity. the endothelial cells of caprine aorta tissue cultured in vitro and exposed to the m. mycoides underwent severe ultrastructural damage. there was no evidence of ... | 1984 | 6511321 |
| vaccination trials against mycoplasma mycoides subsp. mycoides (large-colony-type) infection in goats. | vaccination trials against mycoplasma mycoides subsp. mycoides in suckling and weaned kids are described. a formalin-killed vaccine with adjuvant was used. challenge to the vaccinated suckling kids was by ingestion of dam's infected milk and by subcutaneous inoculation of 10(6) organisms, and to the vaccinated weaned kids, by subcutaneous inoculation alone. the weaned 6-week-old kids were vaccinated, given a booster 3 weeks later and challenged 1 month after that. serological monitoring was by e ... | 1984 | 6511322 |
| role of mycoplasma strain f38 in contagious caprine pleuropneumonia. | the role of the f38 group of mycoplasma in contagious caprine pleuropneumonia is assessed with reference to certain criteria whereby a causative relationship between a particular agent and the disease in question is established. the evidence that mycoplasma mycoides subsp. mycoides (large colony) or m. mycoides subsp. capri may be involved in the disease is similarly considered. | 1984 | 6511324 |
| some in-vitro characters of the subspecies of mycoplasma mycoides. | so-called lc strains of mycoplasma mycoides subsp. mycoides and, where appropriate, sc strains, were examined, together with m. mycoides subsp. capri strains, by growth, fermentation, and antibiotic-sensitivity tests. growth curves in bvf-os medium showed that, from the 6th until at least the 19th day of incubation, strain mankefår 2833 had a viable count strikingly higher than that of any other lc strain. its robust growth properties may explain its ability--unusual among lc strains--to produce ... | 1982 | 6759579 |
| mycoplasma mycoides subsp mycoides, lc type, from goats in italy. | a mycoplasma identified as mycoplasma mycoides subsp mycoides lc type was isolated from an outbreak of caprine pleuropneumonia and serofibrinous peritonitis. this isolate was the first to be reported in italy. experimentally, a rapidly fatal condition followed its inoculation into goats and sheep but inoculation of calves did not lead to clinically apparent infection. although the organism was recovered for up to 45 days following experimental inoculation of calves, pathological changes were lim ... | 1982 | 6760527 |
| a passive haemagglutination test for detection of antibodies against mycoplasma mycoides subsp. mycoides using glutaraldehyde-fixed sheep erythrocytes. | a simple passive haemagglutination test using the microtitre technique was developed for the detection of antibodies against mycoplasma mycoides subsp. mycoides infection in cattle. of the four different concentrations (0.1, 0.2, 0.25 and 0.5%) of glutaraldehyde used for the fixation of sheep erythrocytes, antigens prepared with erythrocytes fixed with 0.2 and 0.25% concentrations of glutaraldehyde gave the best results. the test was found to be very practical, sensitive specific and reproducibl ... | 1982 | 6817507 |
| pathogenicity of a caprine strain of mycoplasma mycoides subsp. mycoides for cattle. | | 1980 | 7000862 |
| effect of soluble factors from mycoplasma mycoides subsp. mycoides on the collagen content of bovine connective tissue. | | 1980 | 7000863 |
| further studies on caprine and ovine mycoplasmas related to mycoplasma mycoides subsp. mycoides. | nine caprine and ovine mycoplasma strains, said to be indistinguishable serologically from mycoplasma mycoides subsp. mycoides (the causative organism of contagious bovine pleuropneumonia; cbpp) were examined in mice by (1) a mycoplasmaemia test, and (2) a cross-protection test. of the nine strains, two from goats belonged to a small colony (sc) type; four caprine and three ovine strains belonged to a large colony (lc) type.the two sc strains - like a single sc strain examined in an earlier stud ... | 1980 | 7005327 |
| experimental infection of goats, sheep and calves with the large colony type of mycoplasma mycoides subsp. mycoides. | goats, sheep and calves were inoculated intravenously with strain y3343 of the large colony type of mycoplasma mycoides subsp. mycoides isolated from a goat with polyarthritis. the goats and sheep died of septicemia (one was killed in extremis) within eight days. the goats had leukopenia and granulocytopenia. coagulopathy was indicated in some goats; the fibrinogen titer, prothrombin and partial thromboplastin times increased with the progress of disease and the number of platelets decreased dra ... | 1981 | 7008331 |
| immunogenic stability of heat-killed mycoplasma mycoides subsp. mycoides at 4 degrees c. | | 1980 | 7016253 |
| observations on mycoplasma mycoides subsp. mycoides infection in saanen goats. | an epizootic in white saanen goats, caused by mycoplasma mycoides subsp. mycoides is described. twenty-five flocks totalling approximately 4,500 animals were involved. the disease was characterized by a high, transient temperature, general malaise and mastitis in the lactating does, and a keratoconjunctivitis, arthritis, mycoplasmaemia and death among the kids. in one goat flock there was a precipitous change in the character of the disease, from a predominantly mastitis syndrome to a fulminatin ... | 1981 | 7026494 |
| the ability of mycoplasma mycoides subspecies mycoides and closely related strains from goats and sheep to immunize mice against subspecies capri. | small colony (sc) strains of mycoplasma mycoides subsp. mycoides from contagious bovine pleuropneumonia (cbpp) and from goats were compared with large colony (lc) strains of so-called m. mycoides subsp. mycoides from goats and sheep by means of a cross-protection test in which mice were challenged with m. mycoides subsp. capri. of 13 lc strains, all gave partial cross-protection, and 11 were shown to be more closely related than four sc strains to subspecies capri. in a further experiment, six s ... | 1981 | 7026674 |
| observations on the antigenic differences between the so-called sc and lc strains of mycoplasma mycoides subsp. mycoides. | the so-called sc (small colony) and lc (large colony) strains of mycoplasma mycoides subsp. mycoides are said to be indistinguishable by the in vitro serological tests of generally used in mycoplasmology. in mice the immunity given by a single dose of killed lc-strain vaccine against challenge with sc strains is - unlike that given by sc-strain vaccine - only partial. when multiple doses of killed or living vaccines were given, the majority of 13 lc strains still failed to immunize completely ag ... | 1981 | 7031127 |
| sequence analysis of 16s rrna from mycoplasmas by direct solid-phase dna sequencing. | automated solid-phase dna sequencing was used for determination of partial 16s ribosomal dna sequences of mycoplasmas. the sequence information was used to establish phylogenetic relationships of 11 different mycoplasmas whose 16s rrna sequences had not been determined earlier. a biotinylated fragment corresponding to positions 344 to 939 in the escherichia coli sequence was generated by pcr. the pcr product was immobilized onto streptavidin-coated paramagnetic beads, and direct sequencing was p ... | 1994 | 7521158 |
| identification and characterization of is1296 in mycoplasma mycoides subsp. mycoides sc and presence in related mycoplasmas. | is1296, a new insertion sequence belonging to the is3 family of insertion elements has been identified in mycoplasma mycoides subsp. mycoides (mmm) biotype small colony (sc), the agent of contagious bovine pleuropneumonia (cbpp). is1296 is 1485-bp long and has 30-bp inverted repeats. it contains two open reading frames, orfa and orfb, which show significant similarities to the orfs which encode the transposase function of is elements of the is3 family, in particular is150 of escherichia coli. is ... | 1995 | 7628725 |
| [the epidemiology of contagious lung disease in switzerland]. | although contagious bovine pleuropneumonia (cbpp) has not been diagnosed in switzerland since 1895, anti-cbpp antibodies were detected in several cattle that had been imported from switzerland to italy. in order to clarify the current disease situation in switzerland, detailed epidemiological investigations were performed, including the control of 200,000 slaughter cattle in 108 abattoirs and serological testing of more than 2200 animals from 134 farms. the data collected in the slaughterhouses ... | 1995 | 7660094 |
| cloning and characterization of the lipase operon from mycoplasma mycoides subspecies mycoides lc. | lipases, serine esterase enzymes, play an essential role in the mycoplasmal nutritional requirement for long-chain fatty acids. although the lipase(s) activity in different mycoplasma species has been investigated, the molecular biology of the corresponding genes has not been studied. using a single-primer pcr technique combined to more classical cloning systems, an operon containing three open reading frames (orf), each of which could encode a lipase protein of 264, 264 or 269 amino acids (aa), ... | 1995 | 7789792 |
| mycoplasma infection in a commercial goat dairy caused by mycoplasma agalactiae and mycoplasma mycoides subsp. mycoides (caprine biotype). | a commercial dairy goat herd of 600 animals experienced sudden onset of arthritis/polyarthritis, clinical mastitis, and sudden death in does. the offending infectious agents were mycoplasma agalactiae and m. mycoides subsp. mycoides (caprine biotype). the disease syndrome began approximately 4 weeks following the 1) introduction into the herd of a lactating doe with no apparent clinical signs and 2) a breakdown of proper hygienic conditions in the milking parlor. over a period of 3 weeks, 90 doe ... | 1994 | 7858021 |
| transformation of mycoplasma capricolum and examination of dna restriction modification in m. capricolum and mycoplasma mycoides subsp. mycoides. | plasmids pik delta and pik delta-erm have recently been developed as mycoplasmal cloning vectors. in this report, we demonstrate that these plasmids can replicate in mycoplasma capricolum, a mycoplasmal species for which transformation had not previously been characterized. both plasmids are stably maintained at a higher copy number than in their parental species, mycoplasma mycoides subsp. mycoides. we have also examined the possibility of one or more restriction-modification systems affecting ... | 1994 | 8058824 |
| cloning and characterization of the reca genes from mycoplasma pulmonis and m. mycoides subsp. mycoides. | the reca protein has a central role in dna repair and is essential for homologous recombination in most eubacteria. little is known about these critical processes in mycoplasmas. by using standard and inverse polymerase chain reactions (pcr) coupled with conventional cloning techniques, a series of overlapping fragments comprising the entire reca genes of mycoplasma mycoides subsp. mycoides (mm) and mycoplasma pulmonis (mp) were generated. each gene was sequenced in its entirety. the reca genes ... | 1994 | 8112579 |
| mycoplasmal cloning vectors derived from plasmid pkmk1. | only two plasmids have been isolated and characterized from the entire genus mycoplasma, which includes over 90 recognized species. both of these plasmids were obtained from the same species, mycoplasma mycoides subsp. mycoides. we have previously characterized one of these plasmids, pkmk1, as a preliminary step in developing mycoplasmal cloning vectors. in the present study, we have separately combined pkmk1 with two different escherichia coli replicons and a tetracycline resistance (tetm) gene ... | 1994 | 8171125 |
| the identification of mycoplasma mycoides mycoides lc as the aetiological agent of balanoposthitis and vulvovaginitis in sheep in south africa. | clinical ulcerative balanoposthitis and vulvovaginitis was experimentally reproduced in 14 sheep infected with a mycoplasma mycoides mycoides lc field strain, isolated from the straussheim dorper stud. the study encompassed a series of field observations, a therapeutic trial and experimental investigations. a wide range of bacteria and various mycoplasma spp., but no viruses, were isolated from a large number of infected animals. | 1993 | 8392679 |
| selective acylation of plasma membrane proteins of mycoplasma mycoides subsp. mycoides sc, the contagious bovine pleuropneumonia agent. | the plasma membrane of mycoplasma mycoides subsp. mycoides sc (strain kh3j) contains over 160 polypeptides with apparent molecular masses ranging from 14 to 125 kda and isoelectric point values (pis) from 5 to 9. in vivo labeling with [14c]-fatty acids revealed about 35 acylated polypeptides including the two major components p42 and p65 and displaying pis between 5.5 and 9.0, with a majority between 6.5 and 8. the amphiphilic nature of most of these acyl proteins was confirmed by triton x-114 p ... | 1996 | 8555942 |
| insertion element is1296 in mycoplasma mycoides subsp. mycoides small colony identifies a european clonal line distinct from african and australian strains. | strains of mycoplasma mycoides subsp. mycoides small colony (sc) type, the agent of contagious bovine pleuropneumonia (cbpp), were analysed with respect to the polymorphism of distribution of a newly discovered insertion element, is1296, on the chromosome. analysis of 64 strains isolated from europe, africa and australia, including four vaccine strains and the type strain pg1, revealed ten different is patterns, forming two main clusters. the european strains originated from outbreaks of cbpp in ... | 1995 | 8574413 |
| surveillance of contagious bovine pleuropneumonia in switzerland. | an active surveillance system for contagious bovine pleuropneumonia (cbpp) has been established in switzerland. the system is based on the detection of typical gross pathological lesions in the lungs of slaughtered cattle, followed by microbiological analysis of the sampled organs and sero-epidemiological investigation of the herd of origin for each suspect case. the programme was tested over six months. the prevalence of lung lesions detected in carcasses during this period in the 108 participa ... | 1995 | 8593396 |
| [complement fixation reaction for the serologic diagnosis of bovine contagious pleuropneumonia: application and interpretation of the results]. | contagious bovine pleuropneumonia (cbpp), caused by mycoplasma mycoides subsp. mycoides sc (small colony variant), is a disease which has been recognised for a long time. serological testing is used most often for diagnosing cbpp in live animals. this method is based on a complement fixation test (cft), the technique recommended by the office international des epizooties, which has an important role in detecting infected herds in regions known to be affected by the disease. although this serolog ... | 1995 | 8593397 |
| high mortality in goats associated with the isolation of a strain of mycoplasma mycoides subsp. mycoides (large colony type). | a goat pleuropneumonia outbreak occurring in a herd of 800 animals in the province of ciudad real, spain, is described in this paper. severe respiratory signs and high mortality were the most significant clinical observations. the adult goats presented mainly respiratory symptoms and/or mastitis, whereas the young animals died showing arthritis and/or keratoconjunctivitis. the most significant lesions were found in the thoracic cavity. a focal extensive fibrinonecrotic pleuropneumonia was macros ... | 1995 | 8594844 |
| molecular epidemiology of mycoplasma mycoides subsp. mycoides biotype small colony, the agent of contagious bovine pleuropneumonia. | restriction endonuclease analysis (rea) and immunoblotting were used to compare field strains of mycoplasma mycoides subsp. mycoides biotype small colony from different parts of europe and africa. of the 27 strains tested by rea, the european ones (n = 15), except 3 from the iberian peninsula, differed strikingly from the african ones (n = 10). vaccinal strains t1-sr and kh3j had a unique rea pattern. polyclonal immunoblot profiles of 46 strains appeared fairly similar. however most of the itali ... | 1995 | 8748546 |
| a pathological and immunohistochemical study of caprine pleuropneumonia induced by subspecies of mycoplasma mycoides. | in a population of 700 goats, 150 died; of these, 29 were necropsied. ten of the 29 goats had pleuropneumonia. mycoplasma mycoides subsp. mycoides (large colony) (mmmlc), mycoplasma mycoides subsp. capri (mmc), and pasteurella multocida were isolated from five of the pleuropneumonic goats. gross and microscopical lesions were typical of caprine pleuropneumonia (cpp), with bronchopneumonia, fibrinopurulent or fibrinonecrotic pleuropneumonia and dilatation of the interlobular septa and pleura. imm ... | 1996 | 8814532 |
| detection of mycoplasma mycoides subspecies mycoides by monoclonal antibody-based sandwich elisa. | monoclonal antibodies (mabs) were produced from a mouse immunised with mycoplasma mycoides subsp. mycoides small colony (mmmsc) antigen and their use to detect and differentiate strains within the mycoplasma mycoides cluster investigated in an antigen capture elisa format. the mabs produced could not distinguish between mmmsc and m. mycoides subsp. mycoides large colony (mmmlc) strains. however, the sandwich elisas developed were able to specifically distinguish these two biotypes from the other ... | 1996 | 8828123 |
| monoclonal antibodies to surface-exposes proteins of mycoplasma mycoides subsp. mycoides (small-colony strain), which causes contagious bovine pleuropneumonia. | outbreaks of bovine pleuropneumonia caused by small-colony strains of mycoplasma mycoides subsp. mycoides occur in africa, and vaccination is used for control. since protein subunits are needed to improve multivalent vaccines, monoclonal antibodies (mabs) were made to facilitate protein identification and isolation. eleven immunoglobulin m mabs derived from mouse spleen donors immunized with disrupted whole organisms bound periodate-sensitive epitopes on externally exposed polysaccharide. seven ... | 1996 | 8914769 |
| contagious bovine pleuropneumonia in northern tanzania, culture confirmation and serological studies. | after an absence of about 25 years contagious bovine pleuropneumonia (cbpp) appeared again in 1990 in tanzania. it was preceded by a spread in kenya to an area bordering tanzania. due to the frequent cattle movements across the border it was soon introduced into loliondo in northern tanzania. one month after the first cases, cbpp was suspected in a total of 9 herds comprising 1,500 cattle. however, few animals showed clear clinical signs and frequent antibiotic treatment at an early stage furthe ... | 1995 | 8966759 |
| characterization of the gene for an immunodominant 72 kda lipoprotein of mycoplasma mycoides subsp. mycoides small colony type. | with the aim of characterizing specific immunogenic proteins of mycoplasma mycoides subsp. mycoides small colony (sc) type, the aetiological agent of contagious bovine pleuropneumonia, a gene encoding a major immunogenic protein of 72 kda named p72 was cloned and expressed in escherichia coli. the expressed protein was of the same apparent molecular mass as that produced by the parent strain. the predicted molecular mass of p72, based on the dna-deduced amino acid sequence, was 61.118 kda, signi ... | 1996 | 9004514 |
| a 13-kda protein with a helix-turn-helix motif is encoded by bacterial operons related to the srp pathway. | we have identified a 13 kda protein (p13) in mycoplasma mycoides subsp. mycoides that is encoded immediately downstream of a protein homologous to e. coli ftsy, a protein taking part in the bacterial signal recognition particle (srp) pathway. the same organisation of the p13 and ftsy genes occurs in mycoplasma pneumoniae. pcr analysis of different mycoplasma strains revealed the same organisation in strains belonging to the mycoplasma mycoides cluster of the mycoplasma phylogenetic tree. searche ... | 1997 | 9070906 |
| virulence of mycoplasma mycoides mycoides (sc) | | 1997 | 9106979 |
| development of a selective polymerase chain reaction assay for the detection of mycoplasma mycoides subsp. mycoides s.c. (contagious bovine pleuropneumonia agent). | a new selective assay for the detection of mycoplasma mycoides subsp. mycoides sc (mmmsc) via the polymerase chain reaction (pcr) has been developed. this test used two pcr assays: a control-pcr (myc-pcr) identifying the pathogen as a member of the mycoides cluster and the msc-pcr which is specific for mmmsc. the myc primers targeted a dna sequence of about 460 bp from all the 59 mycoides cluster-strains tested. no amplification occurred with bovine genomic dna or with the 11 other bacterial spe ... | 1994 | 9133058 |
| development of a sensitive nested pcr method for the specific detection of mycoplasma mycoides subsp. mycoides sc. | a specific and sensitive test for the detection of mycoplasma mycoides subsp. mycoides small colony type (sc), the aetiological agent of contagious bovine pleuropneumonia (cbpp) was developed using two nested pcr reactions. the pcr reactions are based on the nucleotide sequence of lipoprotein p72 of m. mycoides subsp. mycoides sc. the two specific oligonucleotide primer pairs were chosen to match those sequence segments of the p72 gene which differ most from the gene of the closely related lipop ... | 1997 | 9160324 |
| polymerase chain reaction and restriction endonuclease digestion for selected members of the "mycoplasma mycoides cluster" and mycoplasma putrefaciens. | a specific diagnostic method using the polymerase chain reaction, together with restriction endonuclease digestion patterns, was developed for members of the "mycoplasma mycoides cluster" that normally occur in the united states (i.e., mycoplasma mycoides subsp. mycoides large colony and mycoplasma capricolum subsp. capricolum in addition to "cluster" mycoplasma, bovine serogroup 7, and mycoplasma putrefaciens. the digestion of "cluster" polymerase chain reaction dna (1,225 bp) amplification pro ... | 1997 | 9211239 |
| preliminary comparative studies on complement fixation, dot enzyme immunoassay, and western blotting for the detection of antibodies to mycoplasma mycoides subspecies mycoides sc in nigerian camel (camelus dromedarius). | the serological prevalence of antibodies to mycoplasma mycoides subsp. mycoides sc, the aetiological agent of contagious bovine pleuropneumonia (cbpp), was investigated in nigerian camels. fifty-eight serum samples were collected from slaughtered camels and examined by complement fixation (cft), dot enzyme immunoassay and western blots. fourteen of the slaughtered camels examined had pneumonic lesions. all sera examined were negative by cft but 7 (12.1%) and 4 (6.8%) were positive by dot enzyme ... | 1997 | 9270134 |
| iscom vaccine against contagious bovine pleuropneumonia (cbpp). 1. biochemical and immunological characterization. | a better vaccine than the existing ones against contagious bovine pleuropneumonia (cbpp) caused by mycoplasma mycoides subsp. mycoides small colony type (mmmsc) would improve the chances for eradication of cbpp. in such an effort, immunostimulating complexes (iscoms) have been prepared from the whole detergent-solubilized cells of mmmsc and characterized biochemically and immunologically. the most efficient detergent for solubilization of the mycoplasma was mega-10 which yielded a high recovery ... | 1997 | 9437824 |
| [strategies for prevention and eradication of contagious bovine pleuropneumonia with or without vaccination]. | contagious bovine pleuropneumonia (cbpp) offers, like rinderpest, the paradox of having been eradicated from some countries (north-western europe, the united states of america) before the nature of the pathogenic agent was known. as a preventive measure, inoculation of pathogenic material was used but success varies due to strategic inadequacies. the same applies to vaccination with more or less attenuated live strains of mycoplasma mycoides subsp. mycoides. many ideas for prophylaxis have been ... | 1996 | 9527413 |