| simulation and prevention of retrovirus--specific reactions by mycoplasmas. | from human mycosis fungoides tumor-derived cell lines, mycoplasma hyorhinis was isolated. this mycoplasma shared the following characteristics with retroviruses: uptake of 3h-uridine, but not of 3h-thymidine in cell culture; banding at 1.16 g/ml sucrose density and partial shift to retrovirus core density position (approximately equal to 1.24 g/ml) after detergent treatment; incorporation of 3h-tmp into high molecular weight material in standard reverse transcriptase assays with the template-pri ... | 1979 | 89623 |
| [identification of various porcine mycoplasmas using epi-immunofluorescence]. | the responses of the field strains and type strains of mycoplasma hyorhinis are compared in epi-immunofluorescence, in growth-inhibition tests, and growth-precipitation tests. the type strains, together with the field strains, could be definitely identified by direct and indirect epi-immunofluorescence. in indirect epi-immunofluorescence, all rabbit antiserums against the strains of mycoplasma hyorhinis tested by us always gave positive cross reactions with all the tested strains of mycoplasma h ... | 1978 | 100927 |
| [sensitivity of bordetella bronchiseptica, pasteurella multocida and mycoplasma hyorhinis strains isolated from pigs towards antibiotics and chemotherapeutic agents]. | | 1978 | 100928 |
| determination of sensitivity of mycoplasma hyosynoviae to tylosin and selected antibacterial drugs by a microtiter technique. | a microtiter technique was used for determination of the sensitivity of mycoplasma hyosynoviae to antibiotics and other drugs. use of a biphasic agar-broth medium in microtiter plates allowed direct visualization of growth. results were more reproducible with this system than when broth alone was used and evaluation based on color change was required. attempts to adapt the test for use with mycoplasma hyorhinis were not successful. minimal inhibitory concentrations of 12 drugs and drug combinati ... | 1975 | 122911 |
| selective association of murine t lymphoblastoid cell surface alloantigens with mycoplasma hyorhinis. | mycoplasma hyorhinis, isolated by isopycnic centrifugation from supernatants of a persistently infected murine t lymphoblastoid cell line, demonstrated the presence of the thy-1.1 differentiation alloantigen and h-2kk histocompatibility antigens. the murine leukemia virus-related gp70 antigen also present on the surface of these lymphoblastoid cells was absent from mycoplasma preparations. quantitative assessment of thy-1.1 present in preparations of m. hyorhinis revealed a specific activity gre ... | 1978 | 212754 |
| interaction of murine t-cell surface antigens with mycoplasma hyorhinis. | | 1979 | 232624 |
| adenosine phosphorylase activity in a mutant hep-2 cell line contaminated with mycoplasm hyorhinis. | metabolic studies in hep-2/mp,mir cells (an adenosine kinase, hypoxanthine phosphoribosyltransferase negative mutant) indicated the presence of adenosine phosphorylase activity. this activity, unknown in established mammalian cell lines, resulted in the glycosidic cleavage of both adenosine and the antiviral drug arabinosyladenine. the activity was observed readily in the presence or absence of the adenosine deaminase inhibitor conformycin. isopycnic separation of [3h] thymidine-labeled dna spec ... | 1977 | 409662 |
| uracil phosphoribosyl transferase activity of mycoplasma and infected cell cultures. | human h.ep-2 and mouse 3t6 cells infected by mycoplasma hyorhinis showed an increase in [3h]uracil uptake and a more than 20-fold increase in the activity of uracil phosphoribosyltransferase (uraprt). uninfected cell cultures gave background levels of this enzyme activity. a survey of 16 strains of mycoplasma showed 13 to possess uraprt activity. rabbit kidney cells (rk13) were infected with eight different strains of four mycoplasma species known to be common cell culture contaminants. seven of ... | 1977 | 560353 |
| [studies on the sensitivity of mycoplasma hyorhinis strains against various antibiotics and against nitrofurazone]. | | 1978 | 666718 |
| differences in incorporation of nucleic acid bases and nucleosides by various mycoplasma and acholeplasma species. | eight species representative of the serological diversity of the mycoplasmatales were tested for their ability to incorporate radiolabeled nucleic acid precursors into acid-insoluble material. cultures in complex growth medium were centrifuged and resuspended in minimal essential medium (eagle). for acholeplasma laidlawii, labeling occurred mainly during the first 4 h of incubation, with substrate saturation at 20 micron. all organisms tested incorporated uracil, adenine, and guanine; none incor ... | 1978 | 681280 |
| in vitro susceptibility of mycoplasma hyopneumoniae and mycoplasma hyorhinis to fifty-one antimicrobial agents. | by a tube dilution assay technique, 51 antimicrobial agents were singly tested against 9 strains of mycoplasma hyopneumoniae and 1 strain of m. hyorhinis for the purpose of obtaining information useful for selecting an agent for testing in vivo against porcine mycoplasmal pneumonia. based on determining minimal inhibitory concentrations and chemically grouping the agents into nine classes, all m. hyopneumoniae strains were found resistant to penicillins and peptides and susceptible to sulfonamid ... | 1978 | 697348 |
| experimental infection of gnotobiotic piglets with mycoplasma hyorhinis and bordetella bronchiseptica. | | 1977 | 842196 |
| isolation of folded chromosomes from mycoplasma hyorhinis. | folded chromosomes were isolated from mycoplasma hyorhinis. when examined by electron microscopy, these molecules show variability of loop size, number of loops, total contour length and degree of twisting of the dna. sedimentation velocity was unaltered after treatment with rnase, proteinase k, sds, temperatures up to 65 degrees c and nacl concentrations from 0.1 m to 4 m. | 1977 | 896468 |
| a serologic variant of mycoplasma hyorhinis recovered from the conjunctiva of swine. | | 1976 | 983893 |
| [experiments to obtain and test vaccines against enzootic pneumonia in swine]. | five-day-old pplo broth cultures of two mycoplasma hyorhinis strains were used to obtain an inactivated vaccine consisting of 75 parts culture, 25 parts aluminum hydroxyde and 0.1 per cent formalin, and a live vaccine of 75 parts culture and 25 parts aluminum hydroxyde. the inactivated vaccine was tested on a total of 320 pregnant pigs and the live one--on 3,150 pigs, aged two-three months on a farm with a record of m. hyorhinis infection resulting in an enzootic pneumonia. in those of the pigs ... | 1976 | 1025843 |
| mycoplasma hyorhinis arthritis of swine: a model for rheumatoid arthritis? | | 1975 | 1105750 |
| [mycoplasms of the swine--a review]. | the mycoplasmas constitute a group of microorganisms placed between bacteria and virus. the name, mycoplasma, is derived from the mycelial morphology of the organisms. the minimal reproductive unit, the elementary body, measures 0.2-0.5 mum. unlike bacteria, mycoplasmas are not confined by a rigid cell wall, but just by a thin membrane. for their cultivation, though common bacteriological technique is adequate, especially enriched media are required. antibiotics, as a rule penicillins, are added ... | 1975 | 1153279 |
| histochemical identification of glycoproteins in pig bronchial epithelium: (a) normal and (b) hypertrophied from enzootic pneumonia. | the glycoproteins in the normal pig bronchial gland are identified by the combined alcian blue (ab)-periodic acid schiff (pas) technique, with the use of sialidase digestion and ab staining either at ph 2-6 or at ph 1-0. in enzootic pneumonia (produced experimentally by infection with mycoplasma hyorhinis) the bronchial gland hypertrophies, mucous and serous cells both increase, in number and size; hence the total glycoprotein content of the gland increases. the distribution of glycoproteins in ... | 1975 | 1159572 |
| antimicrobial activities of 81.723 hfu, a new pleuromutilin derivative. | the new pleuromutilin derivative 81.723 hfu is extremely active against gram-positive organisms such as streptococci, staphylococci, and against mycoplasmas. a number of shigella, klebsiella, and escherichia coli strains were also found to be quite susceptible to this new agent, whereas other gram-negative organisms like pseudomonas aeruginosa, proteus species, and alcaligenes faecalis proved to be naturally resistant to 81.723 hfu. the new compound acts bacteriostatically. bactericidal effects ... | 1975 | 1170807 |
| the effects of mycoplasma hyorhinis and of mycoplasma hyopneumoniae on pig kidney primary tissue culture. | | 1975 | 1192170 |
| [identification of mycoplasma by the growth inhibition of samples isolated from cell cultures]. | cell cultures must be continuously screened for the presence of mycoplasma because, although these microorganisms sometimes pass unnoticed, they may cause chromosomic alterations and interfere with viral replication, antibody and interferon production etc. the international organization for mycoplasmology (iom) recommends the isolation and identification of mycoplasma with a view to the detection of the origin of the infection and the improvement of the quality of the cultures. in this paper, 37 ... | 1992 | 1307416 |
| phylogenetic relationships of three porcine mycoplasmas, mycoplasma hyopneumoniae, mycoplasma flocculare, and mycoplasma hyorhinis, and complete 16s rrna sequence of m. flocculare. | the nucleotide sequence of the 16s rrna gene of mycoplasma flocculare was determined and was compared with the sequence of a related porcine mycoplasma, mycoplasma hyopneumoniae. while the overall level of dna-dna homology was approximately 11%, sequence alignment of the two 16s rrna genes yielded a homology value of more than 95%, emphasizing the highly conserved nature of the 16s rrna gene. multiple sequence alignments with other mollicutes indicated that m. flocculare, m. hyopneumoniae, and m ... | 1992 | 1374621 |
| specificity of oligonucleotide probes complementary to evolutionarily variable regions of 16s rrna from mycoplasma hyopneumoniae and mycoplasma hyorhinis. | mycoplasma is the common name for the smallest free-living microorganisms, the mollicutes. mycoplasma hyopneumoniae is of great importance in veterinary medicine, causing enzootic pneumonia in pigs. m hyorhinis can cause polyserositis and may cause pneumonia in piglets. oligonucleotides complementary to variable regions of 16s rrna from these mycoplasmas were designed and used as probes for detection and identification of these mycoplasmas. the probe complementary to 16s rrna of m hyorhinis gave ... | 1992 | 1374926 |
| detection of mycoplasma contamination through modulation (stimulation or inhibition) of thymidine incorporation by unstimulated mouse spleen cells. | in the present report we describe a rapid and sensitive assay for mycoplasma detection in cell cultures. the assay is based on the ability of contaminated culture supernatants to modulate [3h]tdr incorporation by unstimulated mouse splenocytes. several mycoplasma species (mycoplasma orale, culturable and non-culturable strains of mycoplasma hyorhinis) inhibited [3h]tdr incorporation and permitted the detection of some contaminated cell cultures that would otherwise have escaped detection in assa ... | 1992 | 1640109 |
| analysis of mycoplasma hyorhinis dna in the presence of host cells without growing the mycoplasma axenically. | mycoplasma hyorhinis coisolates with the mitochondria of the cells in which it is carried as an infection. since both mitochondria and mycoplasmas synthesize dna by using the prokaryotic dna polymerase gamma, the use of aphidicolin, which inhibits eukaryotic dna polymerase alpha, allows for selective synthesis of mycoplasmal and mitochondrial dna. the restriction patterns of mitochondria and mycoplasmas can easily be differentiated from each other in mixtures of both dnas. thus, it is possible t ... | 1991 | 1671671 |
| phenotypic switching in mycoplasmas: phase variation of diverse surface lipoproteins. | the ability of some microorganisms to rapidly alter the expression and structure of surface components reflects an important strategy for adaptation to changing environments, including those encountered by infectious agents within respective host organisms. mycoplasma hyorhinis, a wall-less prokaryotic pathogen of the class mollicutes, is shown to undergo high-frequency phase transitions in colony morphology and opacity and in the expression of diverse lipid-modified, cell-surface protein antige ... | 1990 | 1688663 |
| mycoplasma detection in cell culture by concomitant use of bisbenzamide and fluoresceinated antibody. | conditions are presented for application of both bisbenzamide (hoechst 33258) stain and a specific fluoresceinated anti-mycoplasma hyorhinis igg to a single cell culture preparation. this allows the same field on a slide to be viewed for presumptive diagnosis of any cell culture contaminant mycoplasma by bisbenzamide staining and for definitive diagnosis of m. hyorhinis strains using fluoresceinated antibody. the use of this method plus a cultural procedure will permit identification of the "non ... | 1990 | 1694167 |
| fast and simple procedure for the detection of cell culture mycoplasmas using a single monoclonal antibody. | the detection of mycoplasmas in cell cultures is still a problem, especially in those laboratories in which the detection and identification of microorganisms is not established as a routine procedure. in our laboratory, monoclonal antibodies (mabs) have been prepared to acholeplasma laidlawii, mycoplasma hyorhinis, mycoplasma orale, mycoplasma arginini and mycoplasma salivarium. 30 mabs were obtained and one of these, designated ccm-2, was shown to bind to all five mycoplasma species. it also b ... | 1990 | 1697315 |
| specific, high-affinity binding sites for angiotensin ii on mycoplasma hyorhinis. | mycoplasmataceae are known to express various proteins that are similar to those present in mammals. we report a strain of mycoplasma hyorhinis isolated from opossum kidney cells with specific, high-affinity binding sites for human angiotensin ii (kd = 5.1 +/- 1.9 nm). in contrast, two strains of m. hominis revealed no specific binding. these binding sites resembled mammalian angiotensin ii receptors by their high affinity and by their sensitivity to dithiothreitol. however, they are different f ... | 1991 | 1718269 |
| molecular basis of mycoplasma surface antigenic variation: a novel set of divergent genes undergo spontaneous mutation of periodic coding regions and 5' regulatory sequences. | antigenic diversity is generated in the wall-less pathogen mycoplasma hyorhinis by combinatorial expression and phase variation of multiple, size-variant membrane surface lipoproteins (vlps). the unusual structural basis for vlp variation was revealed in a cluster of related but divergent vlp genes, vlpa, vlpb and vlpc, which occur as single chromosomal copies. these encode conserved n-terminal domains for membrane insertion and lipoprotein processing, but divergent external domains undergoing s ... | 1991 | 1721868 |
| mycoplasma hyosynoviae isolation from the upper respiratory tract and tonsils of pigs. | the occurrence of mycoplasma hyosynoviae at different locations of the upper respiratory tract and tonsils of pigs was investigated in herds with problems of arthritis apparently caused by this microorganism. the isolation of m. hyosynoviae was facilitated by the use of a medium selectively suppressing the growth of mycoplasma hyorhinis. m. hyosynoviae was cultured from 106 of 178 tonsils of slaughterhouse pigs from 8 herds but could not be isolated from the mucosa of the nasal cavity or the ora ... | 1991 | 1818502 |
| a mycoplasma hyorhinis protein with sequence similarities to nucleotide-binding enzymes. | we have determined the nucleotide (nt) and deduced amino acid (aa) sequence of a unique 115-kda mycoplasma hyorhinis protein (p115) with an n-terminal region containing a highly conserved consensus sequence characteristics of nt-binding domains of several atpase and gtpase enzymes. however, p115 lacked additional conserved features characteristic of some classes of nt-binding proteins. based on the hydropathy profile of the deduced aa sequence, the absence of a leader peptide, its exclusive part ... | 1991 | 1825306 |
| continuous distribution of mycoplasma genome sizes. | genome sizes of eleven strains of eight species of mollicutes mycoplasmataceae were investigated by pulsed-field gel electrophoresis. mycoplasma genomic sizes were determined from the sum of the sizes of fragments obtained after digestion of genomic dna with restriction endonucleases. the sizes of the fragments were determined by comparison of their electrophoretic mobilities with those of lambda dna concatemers. specific restriction endonucleases were chosen so that after digestion three to ten ... | 1991 | 1841633 |
| sequence and tnphoa analysis of a mycoplasma hyorhinis protein with membrane export function. | proteins translocated across the single plasma membrane of mycoplasmas (class mollicutes) represent important components likely to affect several interactions of these wall-less microbes with their respective hosts. however, identification and functional analysis of such proteins is hampered by the lack of mutational systems in mycoplasmas and by a perceived limitation in translating recombinant mycoplasma genes containing uga (trp) codons in other eubacteria. here we directly analyze a gene enc ... | 1991 | 1848219 |
| the vlp system of mycoplasma hyorhinis: combinatorial expression of distinct size variant lipoproteins generating high-frequency surface antigenic variation. | isogenic populations of mycoplasma hyorhinis undergo in vitro high-frequency phase variation in the expression of surface lipoproteins; these products also vary markedly in size through changes in periodic protein structure (r. rosengarten and k.s. wise, science 247:315-318, 1990). in this report, we rigorously define three distinct translation products comprising the vlp (variable lipoprotein) system of m. hyorhinis sk76 and establish parameters of vlp structural diversity and expression that d ... | 1991 | 1856172 |
| [the pathology and pathogenesis of experimental mycoplasma hyorhinis infection of piglets with and without thermomotoric stress. 1. pathologico-anatomic, histologic and immunomorphologic study results]. | the effect of an experimental mycoplasma (m.) hyorhinis infection (3 times intranasal instillation of 3-5 ml bouillon with 10(7) colony forming units [cfu]/ml) in combination with a standard thermomotoric stress (swim-test) was studied in piglets using pathomorphological, immunomorphological and microbiological methods. the 92 piglets were divided into the following 5 groups: swimmers with infection (s-i; n = 19); swimmers without infection (s; n = 19); control piglets with infection (k-i; n = 2 ... | 1991 | 1907790 |
| [the pathology and pathogenesis of experimental mycoplasma hyorhinis infection of piglets with and without thermomotor stress. 2. electron microscopic study results]. | electron microscopic investigations on the respiratory tract of piglets with and without mycoplasma hyorhinis infection (10th day of life) partly combined with swim stress (15 degrees c water temperature) (n = 20/20) yielded the following results: colonization of mycoplasma hyorhinis in the ciliary zone of trachea and bronchi in 15 out of 40 piglets (37.5%); the evidence rate of mycoplasma hyorhinis in pneumonic lungs (8 out of 12 = 66.7%) was significantly higher than in nonpneumonic lungs (7 o ... | 1991 | 1910234 |
| an abattoir survey of pneumonia and pleuritis in slaughter weight swine from 9 selected herds. ii. enzootic pneumonia of pigs: microbiological findings and their relationship to pathomorphology. | lungs from 191 slaughter pigs with gross lesions indicative of enzootic pneumonia of pigs (epp) and 80 grossly normal lungs, all originating from 9 different herds, were subjected to microbiological and pathological examinations. the microbiological studies included both bacterial and mycoplasmal culture and also testing for mycoplasma hyopneumoniae antigen in tissue by indirect immunofluorescent technique. m. hyopneumoniae, pasteurella multocida and mycoplasma hyorhinis were detected in 83%, 43 ... | 1991 | 1950853 |
| identification and characterization of proteinase k-resistant proteins in members of the class mollicutes. | proteins resistant to proteinase k are rare because of the potency, wide ph optimum, and low peptide bond specificity of this enzyme. previously, only the prion proteins associated with transmissible spongiform encephalopathies, possibly related proteins in the mollicute spiroplasma mirum, and proteinase k itself have been reported. we identified a new proteinase k-resistant protein, p40-pr, in two strains of mycoplasma hyorhinis and in extracts of these organisms. p40-pr's are similar to prion ... | 1991 | 1997407 |
| [study on the swine glomerulopathy resembling human iga nephropathy]. | naturally occurring swine glomerulopathy was investigated and its glomerular tissue injury was compared with that of human iga nephropathy. mild proteinuria (30%) and microhematuria (17%) was found in 30 six-month-old swine. serum creatinine level was 1.8 +/- 0.4 (mean +/- sd) mg/dl. light microscopy (lm) disclosed diffuse or focal glomerulopathy with mesangial enlargement and hemispherical deposits in six-month-old swine. electron microscopy revealed dense deposits in the mesangial, para-mesang ... | 1991 | 2051646 |
| evaluation of different hybridization procedures for the detection of mycoplasma contamination in cell cultures. | cell culture samples were analysed for mycoplasma contaminations with two different dna probes which have been described earlier. one probe (the h900 probe), derived from the 23s rrna gene of mycoplasma hyorhinis, cross-hybridized with virtually all mycoplasmas (including the acholeplasmas). the other probe (the t2 probe), derived from a protein gene of acholeplasma laidlawii, cross-hybridized with most acholeplasmas. the two probes were compared in three different direct filter hybridization pr ... | 1990 | 2107395 |
| mycoplasmas produce double-stranded ribonuclease. | mycoplasmas (mollicutes) constitute a constant threat as insidious contaminants of animal cell cultures. they are responsible for myriad biochemical reactions associated with the cells they infect, and undoubtedly have been the source of metabolic and physiological activities attributed to their hosts. in an attempt to demonstrate a dsrna-inducible double-stranded ribonuclease (dsrnase) in mammalian cells, comparable to that reported in avian cells, we discovered high levels of dsrnase "induced" ... | 1990 | 2162846 |
| multiple translational products from a mycoplasma hyorhinis gene expressed in escherichia coli. | we analyzed protein expression from a cloned mycoplasma hyorhinis genomic fragment that produces in escherichia coli a set of related polypeptides of 110, 100, 65, and 55 kilodaltons from a coding region of just over 3.0 kilobases. expression of these multiple products resulted from a mechanism operating at the translational level but not from truncation at uga termination codons, which are known to encode tryptophan in several mycoplasma species. the structural relatedness of the proteins was d ... | 1990 | 2188946 |
| survival of mycoplasma hyorhinis in trypsin solutions. | the survival of four strains of mycoplasma hyorhinis in stock solutions of trypsin was tested at 22, 4 and -15 degrees c. low (10(4)-10(5) cfu/ml) and high (10(6)-10(7) cfu/ml) initial concentrations of each strain were used, each was tested three times. a regular decrease of low and high concentrations (1 log in 10 and 20 min, respectively) was seen at 22 degrees c. at 4 degrees c the low concentrations showed a reduction of about 1 log/h, while apart from one strain high concentrations hardly ... | 1990 | 2198063 |
| microbiological cultivation of mycoplasma hyorhinis from cell cultures. | the failure of many cell culture isolates of mycoplasma hyorhinis to grow on microbiological media has stressed the need for alternate assays to detect these organisms. the use of freshly prepared yeast extract in mycoplasmal media together with incubation in 5% co2/air successfully detected m. hyorhinis in 12 of 12 infected cultures. these were not detected by the use of conventional mycoplasmal media using aerobic or anaerobic incubation. this assay may also be helpful in detection of other my ... | 1990 | 2307642 |
| a mycoplasmal protein influences tumour cell invasiveness and contact inhibition in vitro. | fab fragments of a monoclonal antibody directed against p37, a protein associated with the surface of fs9 mouse sarcoma cells, were previously found to inhibit the highly invasive behaviour of fs9 cells in vitro. we show that p37 originates from mycoplasma hyorhinis. infecting various cell lines with the mycoplasma consistently increased their invasiveness when confronted with chicken heart fibroblasts using abercrombie's confronted explant technique. conversely, removing the mycoplasmas or bloc ... | 1990 | 2384527 |
| [production of a specific gamma globulin against infectious atrophic rhinitis in swine]. | a specific gamma globulin against infectious atrophic rhinitis in swine was produced following a pattern that was worked out for the hyperimmunization of serum-producing swine. used were strains of bordetella bronchiseptica and mycoplasma hyorhinis isolated by the authors. the hyperimmune serum obtained was processed after cohn's method which was improved by ponamarova and nechaeva. the preparation obtained was innocuous and immunogenic for both test animals and pigs. it rendered protection when ... | 1985 | 2409662 |
| sce induction and harlequin staining in mycoplasma-contaminated chinese hamster cells. | chinese hamster v79 and cho cells infected with mycoplasma hyorhinis show elevated sister-chromatid exchange (sce) levels but normal cell proliferation and levels of chromosomal aberrations when compared with uninfected cells. harlequin staining patterns differ from those seen with uninfected cells at similar levels of bromodeoxyuridine (brdurd), indicating that brdurd is rapidly depleted from the medium by the mycoplasmal uridine phosphorylase and therefore becomes unavailable over the two cell ... | 1985 | 2417788 |
| triton x-114 phase fractionation of an integral membrane surface protein mediating monoclonal antibody killing of mycoplasma hyorhinis. | a previously defined immunoglobulin m(kappa) monoclonal antibody reacting with a surface epitope of mycoplasma hyorhinis is shown in this report to mediate specific, complement-dependent mycoplasmacidal activity. immunoblot analysis of mycoplasma components and their tryptic cleavage products showed that the epitope recognized was present on a protein with an apparent molecular weight of 23,000 (p23) and on a limit tryptic fragment of this protein with an apparent molecular weight of 18,000 (p18 ... | 1987 | 2437031 |
| antigenic mimicry of a human cellular polypeptide by mycoplasma hyorhinis. | a 46-kilodalton (kda) polypeptide was immunoprecipitated from radiolabeled extracts of human cell lines infected with mycoplasma hyorhinis by murine monoclonal antibodies pf/2a and ml77. both of these antibodies also reacted in an enzyme-linked immunosorbent assay (elisa) with m. hyorhinis cells and with human and nonhuman cell lines infected with m. hyorhinis but failed to react with a7573 cells infected with any of 10 other species of the order mycoplasmatales. pf/2a also reacted in the elisa ... | 1987 | 2439459 |
| comparison of two methods for detection of mollicutes (mycoplasmatales and acholeplasmatales) in cell cultures in the netherlands. | a total of 1949 cell cultures was tested for contamination with mollicutes by cultivation on and in mycoplasma media, 25.7% of the cell cultures was positive, 243 strains of mycoplasma hyorhinis were isolated. furthermore, mainly m. arginini and m. orale were detected, less often acholeplasma laidlawii, m. fermentans and m. pneumoniae. optimal conditions for isolation were discussed. about one third of 217 hybridoma cultures and two third of 57 myeloma cultures proved to be contaminated, all wit ... | 1987 | 2444156 |
| reiterated dna sequences defining genomic diversity within the species mycoplasma hyorhinis. | genomic restriction fragments isolated from mycoplasma hyorhinis and mycoplasma hyopneumoniae were shown by dna hybridization and nucleotide sequence analyses to contain sequences common to these two species, as well as another porcine-derived mycoplasma, mycoplasma flocculare. intraspecies hybridization experiments using these fragments as probes indicated that the sequence is highly redundant in several strains of m. hyorhinis, but that there is diversity in the sizes of restriction fragments ... | 1988 | 2460718 |
| lipid-modified surface protein antigens expressing size variation within the species mycoplasma hyorhinis. | monoclonal antibodies (mabs) previously shown to recognize distinct epitopes selectively expressed on the surface of some mycoplasma hyorhinis strains were used to define two discrete sets of lipid-modified membrane surface proteins showing marked size variation within this species. sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot analysis of triton x-114 phase-fractionated proteins from six isolates of m. hyorhinis defined a set of amphiphilic integral membrane proteins ... | 1989 | 2462538 |
| human cytomegalovirus and monocytes: limited infection and negligible immunosuppression in normal mononuclear cells infected in vitro with mycoplasma-free virus strains. | human cytomegalovirus (hcmv) infection has previously been associated with the production of immunosuppression. the mechanism by which any such immunosuppressive effect might be mediated is unclear but previous work has implicated an effect of the virus on monocytes. we have attempted to characterize the immunosuppressive activity produced by in vitro infection of normal monocytes with hcmv strain ad169. we first examined the ability of hcmv ad169 and recent clinical isolates to infect normal pe ... | 1989 | 2543753 |
| a mycoplasma genetic element resembling prokaryotic insertion sequences. | nucleotide sequence analysis of two mycoplasma hyopneumoniae-derived copies of a repetitive genetic element revealed structural similarities to typical prokaryotic insertion sequences. this is the first such sequence identified in the class mollicutes. the element spans approximately 1550bp, with 28bp inverted terminal repeats. two open reading frames occur within the sequence, one potentially encoding a protein with a size-variant alpha-helical domain containing heptameric leucine periodicity. ... | 1989 | 2552257 |
| new bacteriophage vectors for the large scale production of single stranded insert dna. | ssev 18 and ssev 19, derivatives of the bacteriophages m13mp18/19, are new versatile cloning vectors allowing the large scale preparation of single stranded (ss) insert dna. replacing the original multiple cloning site by a synthetic 96 bp dna fragment, a new polylinker region has been introduced containing complementary sequences designed to form a stem structure where single stranded insert fragments can be excised via a 'master restriction' site. the usefulness of such a vector has been demon ... | 1989 | 2555405 |
| antigenic mimicry of mammalian intermediate filaments by mycoplasmas. | a murine monoclonal antibody raised against mycoplasma hyorhinis specifically reacted by indirect immunofluorescence with mammalian intermediate filaments. the antibody recognized a related epitope on a 74,000-molecular-weight protein of m. hyorhinis and on components of similar size from other pathogenic mycoplasmas. this defines a shared antigenic structure of interest in autoantibody development during mycoplasmal diseases. | 1985 | 2580796 |
| [fluorescence serological and culture detection of mycoplasma hyopneumoniae and mycoplasma hyorhinis in swine lungs]. | the present paper compares the fluorescence-serological detection of m. hyopneumoniae and m. hyorhinis in frozen sections with cultural procedures. in 161 lungs of swine the agent of enzootic pneumonia, m. hyopneumoniae, was found almost exclusively in frozen sections (22 times). only one strain was cultivable. m. hyorhinis was detected 59 times by culture but only 18 times in the sections. for the detection of m. hyopneumoniae therefore the fluorescence-serological investigation of lung section ... | 1989 | 2647076 |
| reduction in immunoreactivity of varicella-zoster virus proteins induced by mycoplasma contamination. | during the study of protein differences between several strains of varicella-zoster virus (vzv), two of the strains were found to be contaminated with mycoplasma hyorhinis. polyacrylamide gel electrophoresis showed fourteen extra bands present in mrc5 fibroblasts infected with these strains compared to other strains of vzv. a more striking difference was observed when infected cultures were used as antigens in immunoblotting. certain viral proteins, corresponding in molecular weight to the viral ... | 1988 | 2840452 |
| transformation of mycoplasma pulmonis and mycoplasma hyorhinis: transposition of tn916 and formation of cointegrate structures. | a procedure for transformation of the murine pathogen mycoplasma pulmonis with plasmid pam120 was developed. this plasmid replicates in escherichia coli and contains the gram-positive transposon tn916. the transformation protocol also proved effective for the swine pathogen mycoplasma hyorhinis. the tetracycline resistance determinant of tn916 was expressed in transformed myocoplasma cells, and tn916 was found inserted into numerous sites in the recipient chromosomes of m. pulmonis and m. hyorhi ... | 1988 | 2853878 |
| effects of mycoplasma infection on fc receptors for ige of rat basophilic leukemia cells. | earlier studies from this laboratory had shown that rat basophilic leukemia cells carry two major receptors for ige, named r and h, and a third minor receptor, designated 71k. it is now apparent that 71k is induced by the action of mycoplasma hyorhinis, a common contaminant of tissue cultures. this induction is reversible. decontamination either in in vitro or in vivo leads to a disappearance of 71k and re-infection causes its reappearance. the 71k receptor appears to be induced by the action of ... | 1986 | 2946588 |
| mycoplasma-induced degradation of ige bound by fc epsilon receptors of rat basophilic leukemia cells. | previous studies from this laboratory have shown that mycoplasma hyorhinis interacts with some unoccupied, high-affinity 45-kda receptors for ige on rat basophilic leukemia (rbl) cells to induce the formation of 71-kda receptors for ige. the present study demonstrates that when ige is bound to the high-affinity receptors, exposure of rbl cells to mycoplasma leads to a time-dependent degradation of the cell-bound ige into fragments of 186 kda, 158 kda and 115 kda, all of which remain bound to the ... | 1987 | 2953615 |
| conversion of a melanoma cell line from an hla class ii positive to negative phenotype after treatment with anti-mycoplasma drugs. | an hla class ii-positive melanoma cell line, du-mel 17, expressing both dr and dq was shown to be contaminated with mycoplasma hyorhinis. after treatment of the cell line with anti-mycoplasma drugs (a tetracycline derivative and a pleuromutilin), both dr and dq expression on the cell surface, as well as dr alpha mrna, were greatly decreased and the cells were rendered mycoplasma-free. interestingly, drug-treated cells became resistant to geneticin, an unrelated drug. to test whether drug treatme ... | 1988 | 3170288 |
| a mycoplasma high-affinity transport system and the in vitro invasiveness of mouse sarcoma cells. | fs9 mouse sarcoma cells were previously shown to be highly invasive when confronted with chicken heart fibroblasts using abercrombie's confronted explant technique. this invasion could be inhibited by addition to the assay of fab fragments of a monoclonal antibody directed against p37, a protein associated with the surface of fs9 cells. we have cloned and sequenced the gene for p37. we show that it originates from mycoplasma hyorhinis and that uga is a tryptophan codon in this organism. we prese ... | 1988 | 3208756 |
| evidence for high affinity binding-protein dependent transport systems in gram-positive bacteria and in mycoplasma. | gram-negative bacteria are surrounded by two membranes. in these bacteria, a class of high affinity transport systems for concentrating substrates from the medium into the cell, involves a binding protein located between the outer and inner membranes, in the periplasmic region. these 'periplasmic binding-proteins' are thought to bind the substrate in the vicinity of the inner membrane, and to transfer it to a complex of inner membrane proteins for concentration into the cytoplasm. we report evid ... | 1988 | 3208757 |
| suppression of cytotoxic responses by a supernatant factor derived from mycoplasma hyorhinis-infected mammalian cell lines. | a class of interleukin-2-dependent t-cell clones, isolated from a murine fetal thymus, was previously shown to suppress the induction of cytotoxic responses to alloantigens (h.-s. teh, m. ho, and w. r. mcmaster, j. immunol. 135:1582-1588, 1985). in that article, the immunosuppressive properties of these t-cell clones were shown to be a direct consequence of infection by mycoplasma hyorhinis. suppression of cytotoxic responses was mediated by both the mycoplasmas and a 200-kilodalton factor prese ... | 1988 | 3257204 |
| association of lipids with integral membrane surface proteins of mycoplasma hyorhinis. | triton x-114 (tx-114)-phase fractionation was used to identify and characterize integral membrane surface proteins of the wall-less procaryote mycoplasma hyorhinis gdl. phase fractionation of mycoplasmas followed by analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed selective partitioning of approximately 30 [35s]methionine-labeled intrinsic membrane proteins into the tx-114 phase. similar analysis of [3h]palmitate-labeled cells showed that approximately 20 proteins o ... | 1988 | 3338843 |
| curing human hybridomas infected with mycoplasma hyorhinis. | tiamuline and minocycline were evaluated for the treatment of an igm producing human-human hybridoma cell line infected with mycoplasma hyorhinis. tiamuline was used at a concentration of 10 micrograms/ml culture medium and minocycline at a concentration of 5 micrograms/ml culture medium. both antibiotics were found to eliminate mycoplasma infection over a treatment period of 3 weeks, and the hybridoma cell line remained mycoplasma-free for 6 months after treatment. tiamuline had no effect on ei ... | 1988 | 3379313 |
| activities of oxidative enzymes in mycoplasmas. | the activities of several oxidoreductases were measured in three fermentative and two nonfermentative mycoplasma species that were grown under aerobic or anaerobic conditions. acholeplasma laidlawii mg, mycoplasma hyorhinis gdl, and mycoplasma pneumoniae fh had very high apparent activities of pyruvate dehydrogenase and pyruvate dehydrogenase complex compared with the activities of mammalian fibroblasts or human platelet-enriched preparations, while mycoplasma salivarium vv and mycoplasma arthri ... | 1987 | 3571159 |
| a "lymphokine-like" soluble product that induces proliferation and maturation of b cells appears in the serum-free supernatant of a t cell hybridoma as a consequence of mycoplasmal contamination. | the serum-free supernatant of a cloned murine t cell hybridoma supports the proliferation and maturation to ig secretion of purified b cells (mu+ cells) from balb/c nu/nu mice, but has no effect on the proliferation of nylon wool-selected balb/c nu/+ splenic t cells. although the supernatant activates b cells without co-stimulation, it synergizes with anti-mu for the proliferative response. the induction of b cell proliferation and maturation to ig secretion is directly related to contamination ... | 1985 | 3871110 |
| selective detection of mycoplasma hyorhinis using cloned genomic dna fragments. | a 13-kilobase dna fragment from a genomic library of mycoplasma hyorhinis demonstrated specific southern hybridization and dot hybridization when tested against a group of different mycoplasmas. this probe selectively recognized m. hyorhinis sequences in purified dna, broth-grown organisms, and infected cell cultures, providing a direct method for differential detection of this species. | 1985 | 3972456 |
| [polyarthritis in piglets caused by mycoplasma hyorhinis]. | | 1985 | 4024058 |
| mycoplasma hyorhinis swine arthritis. i. clinical and microbiologic features. | | 1971 | 4101208 |
| mycoplasma suipneumoniae and mycoplasma hyorhinis demonstrated in pneumonic pig lungs by the fluorescent antibody technique. | | 1971 | 4102500 |
| identification of mycoplasma hyorhinis and mycoplasma hyosynoviae by immunofluorescence. | | 1972 | 4110170 |
| serum and synovial fluid protein of normal and mycoplasma hyorhinis-infected swine. | | 1972 | 4112406 |
| characteristics of a mycoplasma hyorhinis strain contaminating a human fetal diploid cell line. | | 1969 | 4182353 |
| enzootic pneumonia of pigs: complement-fixation tests for the detection of mycoplasma antibodies in the serum of immunized rabbits and infected swine. | the direct, the modified direct and the indirect complement-fixation tests were investigated as methods for the detection of antibodies for the enzootic pneumonia mycoplasma and for mycoplasma hyorhinis in the serum of infected pigs and of immunized rabbits. only the modified direct complement-fixation test in which the guinea-pig complement is supplemented with fresh, normal unheated calf serum was suitable for the detection of mycoplasma antibodies in sera of infected swine. based on the close ... | 1968 | 4234787 |
| enzootic pneumonia in pigs: propagation of a causative mycoplasma in cell cultures and in artificial medium. | three strains of a new species of mycoplasma were recovered from pneumonic pig lungs, known free of mycoplasma hyorhinis, by prolonged incubation in pig testicle cell cultures. the three strains produced a characteristic cytopathic effect in the cell cultures. a highly enriched meat-infusion-broth medium was evolved and permitted regular propagation of these organisms. pneumonia could consistently be produced by intratracheal inoculation of pigs with the mycoplasma propagated in the enriched bro ... | 1969 | 4237289 |
| glasser's disease of swine produced by the intracheal inoculation of haemophilus suis. | the intracheal inoculation of pigs with haemophilus suis led to the production of glasser's disease at every attempt without significant pulmonary involvement. isolation of this organism from the experimental animals was possible only in the acute phase of the disease. the indirect fluorescent antibody technique when applied to frozen sections of tissues obtained from the experimentally infected pigs at autopsy, revealed a few rod forms but mostly "round bodies" of h. suis in animals from which ... | 1969 | 4242769 |
| enzootic pneumonia in pigs: identification of a causative mycoplasma in infected pigs and in cultures by immunofluorescent staining. | immunofluorescent staining has been used to identify mycoplasma hyopneumoniae in smears of broth cultures, in infected pig testicle cell cultures, and in frozen cut sections of pneumonic lungs from field and experimentally produced cases of enzootic pneumonia. in the pneumonic pig lung, fluorescent staining was limited to the surface of the bronchial and bronchiolar epithelium and to the contained exudate. in a series of trials using experimentally infected pigs fluorescence was not detected unt ... | 1970 | 4246002 |
| the effect of medicated feed on the nasal microflora and weight gain of pigs. | antimicrobial agents were added to the feed of swine for three weeks to determine the interrelationships of potentially pathogenic agents in the nasal tract, turbinate atrophy and weight gains. bordetella bronchiseptica was not isolated from the groups fed the combination of chlortetracycline, penicillin and sulfamethazine. b. bronchiseptica was found in some pigs after the feeding trail, but this organism was not significantly associated with turbinate atrophy at the time of slaughter.mycoplasm ... | 1972 | 4258545 |
| chronological development of mycoplasma hyorhinis and mycoplasma hyosynoviae infections in cultures of a swine synovial cell strain. | the sequential development of mycroplasma hyorhinis and mycoplasma hyosynoviae was observed in cultures of a swine synovial fluid cell strain. an early transitory filamentous phase was observed with m. hyorhinis infection followed by the development of cell-associated, relatively large, round structures and some ring forms. infection with m. hyorhinis was characterized by a generalized distribution of the organism and a severe cytopathic effect. infection with m. hyosynoviae was represented by t ... | 1972 | 4259928 |
| susceptibility and resistance of various strains of mycoplasma hyorhinis to antisera, polymyxins and low ph values. | | 1969 | 4310937 |
| demonostration of mycoplasma hyorhinis and mycoplasma hyosynoviae in lesions of experimentally infected swine by immunofluorescence. | | 1972 | 4550844 |
| evaluation of some serological techniques for the identification of mycoplasma hyorhinis and mycoplasma suipneumoniae. | | 1974 | 4604451 |
| histologic and histochemical characterization of synovial membrane from normal and mycoplasma hyorhinis-infected swine. | | 1972 | 4641208 |
| experimentally induced mycoplasma hyorhinis arthritis of swine: pathologic response to 26th postinoculation week. | | 1973 | 4691484 |
| experimentally induced mycoplasma hyorhinis arthritis of swine: immune response to 26th postinoculation week. | | 1973 | 4691485 |
| chemical and serological properties of mycoplasma hyorhinis fractions. | mycoplasma hyorhinis organisms were fractionated into lipid, carbohydrate, and protein fractions by chemical and physiochemical procedures. when all fractions were tested for complement fixation and inhibition of growth inhibition activity, only the protein fractions possessed activity. thus, it appears that the major antigenic component of m. hyorhinis is a protein. | 1973 | 4716550 |
| mycoplasma hyorhinis swine arthritis. 3. modified disease in piney woods swine. | piney woods miniature swine, when injected with mycoplasma hyorhinis, develop an acute arthritis. the disease in these animals is limited clinically, microbiologically, and pathologically to a mild acute illness without the more chronic and destructive features noted in a previous study of the experimental disease in yorkshire swine. | 1973 | 4784887 |
| the effect of mycoplasma hyorhinis strain s7 on pig tracheal organ cultures. | | 1974 | 4820087 |
| influence of intraperitoneal administration of hyperimmune pig serum, igg and igm on the development of infection in gnotobiotic piglets infected intranasally with mycoplasma hyorhinis. | | 1974 | 4839142 |
| intranasal infection of gnotobiotic piglets with mycoplasma hyorhinis: differences in virulence of the strains and influence of age on the development of infection. | | 1974 | 4844322 |
| the antigenic differentiation of seven strains of mycoplasma hyorhinis by growth-inhibition, metabolism-inhibition, latex-agglutination, and polyacrylamide-gel-electrophoresis tests. | | 1974 | 4854652 |
| potential use of surface-active agents for controlling mycoplasma contamination in animal cell cultures. | seven nonionic detergents, which were determined to be relatively nontoxic to selected animal cell cultures, were tested for their lethal effect on the gdl strain of mycoplasma hyorhinis. of the seven detergents tested, five were found to cause complete lysis of the organism in vitro within 24 hr at 37 c. these detergents included triton wr-1339 and tweens 20, 40, 60, and 80. when different concentrations of the detergents were tested, tween 80 was found to be the most effective and triton wr-13 ... | 1969 | 4888862 |
| comparative study of two different methods of isolating mycoplasma hyorhinis. | | 1970 | 4933785 |
| production of pneumonia after intransal inoculation of gnotobiotic piglets with three strains of mycoplasma hyorhinis. | | 1971 | 4935555 |
| identification of mycoplasma hyorhinis. | | 1971 | 4947489 |
| the passive haemagglutination test for th detection of mycoplasma suipneumoniae and the possible diagnosis of enzootic pneumonia of pigs. | fourteen cases of enzootic pneumonia, nearly all of which had presented diagnostic difficulties using the metabolic-inhibition test, were re-examined using specific pig antisera in the passive haemagglutination test (pha). all proved positive for mycoplasma suipneumoniae, indicating that the test, used in this manner, might be particularly valuable for routine diagnosis.the pha test was also used to demonstrate antibody to m. suipneumoniae in pneumonic tissue and the associated bronchial lymph n ... | 1970 | 4988048 |