| localization of enzymes in ureaplasma urealyticum (t-strain mycoplasma). | ureaplasma urealyticum cells were lysed by osmotic shock or by digitonin. the membrane fraction contained four to ten times as much protein as the cytoplasmic fraction. these values are in large excess of those reported for classical mycoplasmas, suggesting that the ureaplasma membrane fraction was heavily contaminated with proteins derived from the growth medium. the u. urealyticum urease activity was localized in the cytoplasmic fraction, whereas the adenosine triphosphatase activity was local ... | 1977 | 15980 |
| effect of ammonium ion concentration and osmotic pressure on growth of ureaplasma urealyticum (t-strain mycoplasma). | the effect of ammonium ion concentration and osmotic pressure on growth of ureaplasma urealyticum type viii was determined by using a well-buffered broth medium containing 10 mm urea. the addition of nh4cl to the medium at concentrations up to 10 mm did not affect growth; however, addition of larger quantities progressively decreased both the specific growth rate (mu) and the maximum yield of the culture, with concentrations of 80 mm completely inhibiting growth. addition of either 150 mm kcl or ... | 1978 | 26668 |
| morphologic observations of mycoplasmas and neisseria gonorrhoeae in associated growth patterns. | mycoplasma hominis and t-colony mycoplasmas were observed growing among or developing within gonococcal colonies on primary isolation plates streaked for detection of n. gonorrhoeae in urogenital specimens. the frequency of such association of mycoplasmas with gonococci was as high as 84% among patients screened for gonorrhea in several social hygiene clinics of the city of new york, department of health, bureau of venereal disease control. the individual morphology of both mycoplasmal and gonoc ... | 1975 | 46128 |
| isolation of genital mycoplasmas from blood obtained shortly after vaginal delivery. | blood obtained from three hundred and twenty-seven women within a few minutes of vaginal delivery was cultured for genital mycoplasmas (mycoplasma hominis and t mycoplasmas). twenty-six (8%) of the women had genital mycoplasmas isolated from their blood. ten women had blood-cultures which contained m. hominis. fifteen women had blood-cultures which contained t mycoplasmas. both m. hominis and t mycoplasmas were isolated from the blood of one woman. only one of two hundred and seventy-three blood ... | 1975 | 47945 |
| doxycycline treatment and human infertility. | the role of mycoplasmas in infertility was studied in 120 couples. during the twelve months of the study 27 couples (22-5%) conceived. t mycoplasmas were isolated from 63% of these couples, and mycoplasma hominis from 18%, compared with 56% and 13%, respectively, in those who did not conceive. 88, with primary infertility of unascertained cause, took part in a controlled trial with doxycycline. the couples in the trial were allocated randomly to three groups: 30 received doxycycline, 28 received ... | 1975 | 47949 |
| effect of preparatory techniques on the gross morphology of mycoplasma hominis. | a method is presented whereby cells of mycoplasma hominis can be prepared with minimal distortion for electron microscopy. after the addition of glutaraldehyde to broth cultures, incubation is continued for 1 h. the cells are then collected by centrifugation, washed in distilled water, and used for negative-contrast preparations. | 1975 | 52651 |
| complement-mediated killing of acholeplasma laidlawii by antibodies to various membrane components. | mycoplasmas are useful models for biochemical studies of the mechanism of complement-mediated killing by antibodies to various membrane components. the purpose of this study was to determine the membrane antigens involved in immune killing of acholeplasma laidlawii. antibodies to a. laidlawii membrane total lipids, glycolipids, and phospholipids could be induced in rabbits after injection of reaggregates of the purified lipids with mycoplasma hominis protein as the carrier. killing of a. laidlaw ... | 1976 | 61175 |
| experimental infection of the genital tract of female grivet monkeys by mycoplasma hominis. | mycoplasma hominis, a common inhabitant of the mucosae of the genitourinary tract of human and nonhuman primates, was inoculated directly into the uterine tubes of five laparotomized grivet monkeys. a self-limiting acute salpingitis and parametritis developed within a few days in all animals. although there were no clinical signs of overt disease, the gross pathology was characterized by pronounced oedematous swelling and hyperaemia of the tubes and parametria. microscopically, cellular infiltra ... | 1978 | 97224 |
| clinical and microbiological investigation of men with urethritis. | of 377 men attending clinics for the treatment of sexually transmitted disease, 104 had gonococcal urethritis, 72 had definite nongonococcal urethritis, 53 had possible nongonococcal urethritis, and 123 had no urethritis. a purulent urethral discharge was noted in 78% and 14% of patients with gonococcal urethritis and definite nongonococcal urethritis, respectively (p less than 0.001). in contrast, 4% and 64% of men with gonococcal urethritis and definite nongonococcal urethritis, respectively, ... | 1978 | 105054 |
| orogenital contact and the isolation of neisseria gonorrhoeae, mycoplasma hominis, and ureaplasma urealyticum from the pharynx. | men and women who came to clinics in boston underwent pharyngeal examinations, and pharyngeal specimens were obtained for cultures for neisseria gonorrhoeae, mycoplasma hominis, and ureaplasma urealyticum. fifty-one (4.9%) of 1,037 participants had gonococcal pharyngeal infection. m. hominis and u. urealyticum were recovered from the pharynges of 149 (14.3%) and 154 (14.8%) of 1,044 participants, respectively. the history of ever having performed fellatio was associated with pharyngeal infection ... | 1979 | 115097 |
| experimental infection of the genital tract of female grivet monkeys by mycoplasma hominis: effects of different routes of infection. | in a previous study we produced acute salpingitis and parametritis in grivet monkeys by inoculation of mycoplasma hominis directly into the uterine tubes. with the purpose of examining in the same animal model the effect of more natural routes of infection, six female grivet monkeys were inoculated, two by two, with m. hominis by the following methods: (experiment a) into the uterine cavity through the cervical canal; (experiment b) into the uterine cavity by the same route, but after ligature o ... | 1979 | 118928 |
| [immunological structure of the population of erevan with regard to mycoplasma hominis]. | | 1976 | 136853 |
| etiology of nongonococcal urethritis. | chlamydia trachomatis was isolated from the urethra from 48 (42 per cent) of 113 men with non-gonococcal urethritis (ngu), four (7 per cent) of 58 without overt urethritis, and 13 (19 per cent) of 69 with gonorrhea. postgonococcal urethritis (pgu) developed in 11 of 11 men who had c. trum antibody to c. trachomatisis developed. the immunotype specificity of chlamydial antibody corresponded to the immunotype isolated. among culture-negative patients. chlamydial antibody prevalence correlated with ... | 1975 | 165407 |
| search for uro-genital tract infections in patients with symptoms of prostatitis. studies on aerobic and strictly anaerobic bacteria, mycoplasmas, fungi, trichomonads and viruses. | seventy-nine patients with symptoms of nonacute prostatitis and 20 healthy volunteers were examined for uro-genital tract infection with bacteria, mycoplasmas, fungi, trichomonads and viruses. no differences in the results of the bacterial cultures were found between the patients and the controls. in only a few cases were established urinary tract pathogens found, but in no instance were these findings reproducible in later specimens. the cultures of the expressed prostatic fluids and the sample ... | 1975 | 175434 |
| prevalence of nine different micro-organisms in the female genital tract. a comparison between women from a venereal disease clinic and from a health control department. | in a study of the prevalence of nine different micro-organisms in the female genital tract in a swedish population, significantly higher isolation rates occurred among women attending a venereal disease clinic than among those attending a gynaecological health control department. the prevalence of candida albicans, however, was similar in different groups, individual susceptibility being the most important factor. chlamydia trachomatis, trichomonas vaginalis, and mycoplasma hominis occurred conc ... | 1979 | 230884 |
| complement-dependent and complement-independent interactions between mycoplasma hominis and antibodies in vitro. | several in vitro reactions between a strain of m. hominis (no. 4195) and homologous antiserum have been delineated and compared. one complement-dependent and four complement-independent activities of antibody have been studied. the complement-dependent activity was mycoplasmacidal and was inhibited by the presence of arginine in the test medium. the complement-independent antibody-mediated reactions were not mycoplasmacidal and were four in number: (a) agglutination, which was manifested in buff ... | 1975 | 240941 |
| a search for serum antibodies in adie's syndrome. | serum specimens from a large number of patients with adie's syndrome were checked for virus antibody levels: measles hemagglutination inhibition (hi), parainfluenza types i, ii and iii (hi), epstein-barr immunofluorescence, mumps complement fixation (cf), adenovirus (cf), varicella-zoster (cf), herpes simplex (cf), cytomegalovirus (cf), mycoplasma hominis (cf), toxoplasma gondii passive hemagglutination. these antibody levels were compared with specimens from a control group of similar age distr ... | 1977 | 304687 |
| recovery of chlamydia and genital mycoplasma transported in sucrose phosphate buffer and urease color test medium. | urethral swabs from 75 males with urethritis were extracted into tryptose phosphate broth and then equal aliquots were dispensed into vials containing sucrose phosphate buffer (2sp) and urease color test medium (u-9). no antibiotics were present in the media. after transport to the laboratory, the recovery of chlamydia trachomatis and ureaplasma urealyticum was evaluated after inoculation into mccoy's cell cultures and agar medium, respectively. c. trachomatis was recovered from significantly mo ... | 1977 | 319081 |
| treatment of nongonococcal urethritis with rifampicin as a means of defining the role of ureaplasma urealyticum. | | 1979 | 371746 |
| chemotactic activity of l-forms and mycoplasma. | l-forms of streptococcus faecalis, escherichia coli and proteus mirabilis showed significantly less chemotactic activity for normal human leucocytes than did parent bacterial forms which were strongly chemotactic. mycoplasma pneumoniae and mycoplasma hominis did not demonstrate chemotactic activity. | 1977 | 414709 |
| genital mycoplasma infection. intrauterine infection: pathologic study of the fetus and placenta. | mycoplasma infection was present in the fetuses from three spontaneous abortions and in one second-trimester newborn. gross examination revealed in most cases a severely infected placenta and membranes, with a fetus of normal appearance. the fetal infection presumably followed placental involvement and appeared to have been acquired shortly prior to delivery. genital mycoplasmas, ureaplasma urealyticum or mycoplasma hominis, were isolated from the placentas and the fetal tissues, and from the ge ... | 1979 | 474493 |
| aerobic and anaerobic bacterial flora in semen from fertile and infertile groups of men. | semen samples were collected by masturbation under asceptic conditions from men who had sired children within the past 6 mo (group a) and asymptomatic men attending an infertility clinic who had not sired children and whose wives were asymptomatic for infertility (group b). these 109 semen samples were analyzed and cultured for isolation of aerobic and anerobic organisms. overall, 68% of the specimens had positive bacterial cultures: 54% of the samples from group a were positive and 73% from gro ... | 1979 | 485650 |
| [role of mycoplasma hominis in etiology of genital diseases in women]. | | 1977 | 605543 |
| [genital mycoplasma strains as a cause of male infertility]. | mycoplasma hominis, m. fermentans, m. orale, m. gallisepticum and ureaplasma urealyticum are able to reduce the motility of spermatozoa. electron microscopical investigation confirmed an affinity of these mycoplasmas to the structures of the cell-membranes of the spermatozoa. simultaneously there could be observed extensive destructions in spermatozoa. | 1978 | 701070 |
| [sensitivity of mycoplasma hominis to selected antibiotics]. | | 1979 | 544980 |
| comparison of methods for the isolation of genital mycoplasmas from men. | two hundred nine men were studied to determine the optimal method of obtaining cultures for genital mycoplasma. ureaplasma urealyticum (t-mycoplasmas) was isolated from 95 (45.5%) of the participants. urethral cultures obtained by means of urethrogenital calcium alginate swabs identified 82 (86%) of the 95 colonized men. urethral cultures taken with cotton-tipped applicators (76%) urine cultures (27%), and cultures of the coronal sulcus (24%) detected fewer colonized men. all men who were coloni ... | 1976 | 772129 |
| genital mycoplasma in intrauterine contraceptive device users. | the incidence association between genital mycoplasma isolation and copper iud users is reported in this indian study. the study group (iud users) had 24.3% isolation of mycoplasma (16 of 66 subjects) compared with a figure of 11.4 in controls (non-iud users). this was a statistically significant difference (p .001). incidence of t-strains was more in the study group (18.2%) as compared with controls (2.2%) (p .001). large-colony mycoplasmas (mycoplasma hominis) had a statistically similar ... | 1979 | 544494 |
| submandibular adenitis in a newborn caused by mycoplasma hominis. | | 1979 | 440904 |
| preparation of competent single-cell suspensions of mycoplasma hominis tets and mycoplasma salivarium tets for genetic transformation to tetracycline resistance by dna extracted from mycoplamsa hominis tetr. | dna extracted from mycomplasma hominis (sprott strain), resistant to 100 micrograms of tetracycline/ml transformed m. hominis strain h29 and mycoplasma salivarium strain s9, which are sensitive to 2.5 and 5.0 micrograms of tetracycline/ml, respectively, to resistance. the transformants were selected on agar medium containing 10 micrograms of tetracycline/ml. some transformants were resistant also to 20 micrograms of tetracycline/ml, a finding confirming that transformation occurred between homol ... | 1979 | 438545 |
| [mycoplasma hominis and inflammatory diseases of the pelvis. in vitro leukocyte stimulation by exposure to m. hominis]. | | 1975 | 817133 |
| [the importance of mycoplasma in infectious processes (author's transl)]. | mycoplasma pneumoniae, the pathogen responsible for primary atypical pneumonia, has appeared more frequently in recent years. while t-mycoplasma and mycoplasma hominis are found in greater numbers both in nonspecific urethritis and also in the genital flora, t-mycoplasma may also cause the formation of vesical calculi in animal experiments. mycoplasma can also be cultured from joint fluids, tumors and from blood samples from parturient women immediately after the birth. | 1976 | 819817 |
| mycoplasma hominis. a cause of neonatal meningitis. | two cases of mycoplasma hominis meningitis in the newborn are described. these infants demonstrate the need to consider m. hominis as a cause of neonatal meningitis, especially if the gram stain is negative, conventional cultures yield no growth, and there is a history of maternal infection. csf cultures on appropriate medium can quickly confirm the diagnosis. | 1979 | 434911 |
| urethritis due to chlamydia trachomatis. | ninety-five men suffering from gonococcal urethritis were treated and observed. forty-nine developed postgonococcal non-specific urethritis (pgu). seventeen men were demonstrated to be free from pgu after careful observation; these formed a control group. chlamydia trachomatis was isolated from urethral material from 26 (53%) of the pgu group but from none of the controls. this difference was highly significant (p less than 0-001). it confirms that c. tachomatis is a pathogen in the urethra. the ... | 1977 | 871894 |
| mycoplasma infection and infertility. | attempts were made to isolate mycoplasmas from the uterine cervix of infertile women and normal pregnant and nonpregnant women to investigate the relationship of genital mycoplasma infection to infertility. ureaplasma urealyticum was demonstrated in 63% of patients with infertility, 68% of normal pregnant women, and 62% of normal nonpregnant women. the incidence of mycoplasma hominis infection was found to be noticeably lower, with corresponding isolation rates of 10%, 11%, and 6%, respectively. ... | 1979 | 428582 |
| ureaplasma urealyticum and mycoplasma hominis in chlamydial and non-chlamydial nongonococcal urethritis. | urethral specimens from 726 patients with nongonococcal urethritis (ngu) were examined for chlamydia trachomatis, ureaplasma urealyticum, and mycoplasma hominis. chlamydiae were isolated from 35.9% of ureaplasma-positive patients and from 36.5% of ureaplasma-negative patients. ureaplasmas were isolated from 52.5% of chlamydia-positive patients and from 53.1% of chlamydia-negative patients, an observation which contrasts with that of some workers who have suggested that ureaplasmas are significan ... | 1979 | 427513 |
| a double-blind cross-over study of the effect of doxycycline on mycoplasma infection and infertility. | forty-two couples in an infertility clinic were examined for genital infection with mycoplasma hominis or ureaplasma urealyticum (t strains). in 21 couples one or other partner was found to be infected; no chlamydiae, trichomonads, or gonococci were isolated from any of these couples. all patients were then included in a double-blind crossover doxycycline/placebo study lasting 8 months. pregnancy was achieved in one of the 21 couples who were free of infection. of the 21 couples who were infecte ... | 1979 | 380631 |
| [mycoplasma hominis as an agent of respiratory tract diseases]. | respiratory organs of newborn rats inoculated intranasally with broth culture of myc. hominis were studied by the histological, histochemical and immunofluorescence methods. tracheitis and development of purulent and interstitial pneumonia with a hemorrhagic component were revealed 24 hours after the infection. these changes were observed up to the 7th day of the experiment. at the same period a specific fluorescence of the myc. hominis antigen was found by the antibody fluorescent test. the pre ... | 1977 | 912095 |
| detection of mycoplasma hominis and mycoplasma orale in cell cultures by immunofluorescence. | mycoplasma contaminants of animal and human cell cultures were rapidly detected and identified by an indirect immunofluorescent technique. cells suspected of being contaminated by mycoplasmas were grown as monolayers on chamber slides in a culture medium selected to promote mycoplasmal growth. before fixation by acetone, the monolayers were subjected to a hypotonic treatment to cause swelling of the mycoplasmas. detection and identification were then performed by indirect immunofluorescence usin ... | 1978 | 352499 |
| isolation of mycoplasma hominis from blood cultures in patients with postpartum fever. | eight women with postpartum fever are presented in whom mycoplasma hominis was isolated from cultures of their blood. clinical disease consisted of a mild although often prolonged febrile illness, and all but one recovered without appropriate antimicrobial therapy. five patients demonstrated elevated convalescent titers of mycoplasmacidal antibodies. the isolates of m. hominis were recovered from routine blood cultures in the diagnostic bacteriology laboratory using blind subcultures to blood ag ... | 1978 | 341009 |
| a microbiological study of neonatal conjunctivae and conjunctivitis. | to investigate the importance of chlamydiae, ureaplasmas, mycoplasma hominis, and anaerobic bacteria in the pathogenesis of neonatal conjunctivitis in the harrow population conjunctival specimens from 104 infants with conjunctivitis and 104 similar healthy neonates were examined. the incidence of neonatal conjunctivitis was 8-2%, and no case of neomycin-resistant disease occurred during the study. staphylococcus aureus, viridans streptococci, and escherichia coli were the only micro-organisms is ... | 1977 | 336080 |
| morphology, ultrastructure, and mode of division of mycoplasma fermentans, mycoplasma hominis, mycoplasma orale, and mycoplasma salivarium. | the morphology of viable mycoplasma fermentans, mycoplasma hominis, mycoplasma orale types 1 and 2, and mycoplasma salivarium was studied in broth cultures by interference microscopy and in thin sections by electron microscopy. only spherical cells were seen by interference microscopy. m. hominis had a capsule-like outer layer. except for m. orale type 1, mycoplasmas in thin sections were 0.3-1 mum in diameter, with a bounding trilaminar membrane 7.5-10 nm thick. the mycoplasmas contained dna fi ... | 1976 | 977994 |
| isolation of mycoplasma species from infertile patients: an epidemiological contribution. | in this study the presence of mycoplasma species in patients suffering from infertility was examined. 41 women and 37 men were examined, including 31 couples, and ten single women and six single men. samples of vaginal and cervical mucus were taken from each woman, and samples of seminal fluid from each man. identification and typing of the organisms were performed on the basis of microscopic and cultural characteristics and inhibition of growth by specific antibodies. out of the 78 patients exa ... | 1977 | 328400 |
| changes in composition, biosynthesis, and physical state of membrane lipids occurring upon aging of mycoplasma hominis cultures. | during the progression of mycoplasma hominis cultures from the early logarithmic phase to the stationary phase of growth, the total phospholipid content of the cell membranes decreased. measurement of the amount of the various phospholipids during the growth cycle showed that a decrease in the phosphatidylglycerol (pg) content, accompanied by an increase in the phosphatidic acid content, occurred upon aging of the culture. pulse labeling experiments revealed that the pg, once formed, is relative ... | 1975 | 234420 |
| prevalence of mycoplasma hominis in patients with gynecologic diseases and abortion in the 2nd--3rd month of pregnancy. | the presence of mycoplasma hominis was investigated in 100 patients with gynecologic diseases, 20 infertility cases and 50 cases of abortion in the 2nd--3rd month of pregnancy. m. hominis could be isolated from the vaginal secretions of 20 patients with gynecologic diseases and of 3 infertility cases. eighteen m. hominis isolates were recovered from 13 patients with abortion (8 strains from the placenta and 10 from vaginal secretions). high titers (1/64--1/512) of complement fixing antibodies to ... | 1978 | 749335 |
| early phases of degeneration of mycoplasma-like bodies in plant tissue cultures infected with aster yellows: morphological analogies with mycoplasma hominis. | several forms of mycoplasma-like bodies (mlbs) were observed in electron micrographs of phloem cells from explants infected with aster yellows disease. the structures were found only in explants 11--25 days old, when the degenerating mlbs were first observed. they resembled those described by anderson and barile in 1965 for mycoplasma hominis. a sequence of events in the degeneration of mlbs is postulated and morphological analogies with degenerating m. hominis are discussed. | 1978 | 709434 |
| mycoplasmas in human pyelonephritis: demonstration of antibodies in serum and urine. | this study was carried out to elucidate by serological examination the etiological significance of mycoplasmas isolated from the upper urinary tract of patients with pyelonephritis. the occurrence of antibodies in patients with acute pyelonephritis, chronic pyelonephritis with or without exacerbation, or noninfectious urinary tract disease was compared by the indirect hemagglutination method. antibody response was demonstrated significantly more often in patients yielding growth of mycoplasma ho ... | 1978 | 701462 |
| differences in incorporation of nucleic acid bases and nucleosides by various mycoplasma and acholeplasma species. | eight species representative of the serological diversity of the mycoplasmatales were tested for their ability to incorporate radiolabeled nucleic acid precursors into acid-insoluble material. cultures in complex growth medium were centrifuged and resuspended in minimal essential medium (eagle). for acholeplasma laidlawii, labeling occurred mainly during the first 4 h of incubation, with substrate saturation at 20 micron. all organisms tested incorporated uracil, adenine, and guanine; none incor ... | 1978 | 681280 |
| occurrence of mycoplasmas in urinary tracts of patients with acute pyelonephritis. | mycoplasma hominis was isolated from the upper urinary tract in 7 of 80 patients with acute pyelonephritis and from 0 of 60 patients with noninfectious diseases of the urinary tract, a significant difference. in four cases m. hominis was isolated in pure culture, in one it was isolated together with ureaplasma urealyticum, and in two it was isolated with bacteria. u. urealyticum was isolated from the upper urinary tract of five patients, all with acute pyelonephritis; this was not significantly ... | 1978 | 670389 |
| septic arthritis due to mycoplasma hominis. a case report and review of the literature. | | 1978 | 659469 |
| serologic studies of human genital mycoplasmas: distribution of titers of mycoplasmacidal antibody to ureaplasma urealyticum and mycoplasma hominis in pregnant women. | titers of mycoplasmacidal antibody to the human genital mycoplasmas ureaplasma urealyticum and mycoplasma hominis were determined using genital isolates from pregnant patients as antigens and comparing these isolates with the 11 prototypic reference strains for u. urealyticum and the seven reference strains for m. hominis. virtually all titers that were detected with use of the patient's own isolates were detected by the 11 reference strains of u. urealyticum and by the seven reference strains o ... | 1978 | 632625 |
| [the importance of ureaplasma urealyticum in non-specific prostato-urethritis (author's transl)]. | 312 patients with non-specific prostato-urethritis were investigated with a standardised procedure using the four glass test. in 42.3% of patients mycoplasma was isolated which in almost all cases was ureaplasma urealyticum. clinically patients were symptom-free after eradication of mycoplasma in cases where previously urea-plasma counts of more than 10,000 cfu/ml prostatic secretion and of more than 1000 cfu/ml urine had been demonstrated. in cases where there were smaller counts of ureaplasma ... | 1978 | 631031 |
| [mycoplasma hominis as a possible agent inducing respiratory tract infections]. | | 1978 | 567535 |
| [detection of mycoplasma pneumoniae infections in female sex organs]. | in the course of isolation experiments on 18 gynaecological patients mycoplasma hominis was found in the genital organs of five and mycoplasma pneumoniae in those of three other women. in 2 cases both agents were present. antibodies against mycoplasma pneumoniae, mycoplasma hominis and mycoplasma orale were in 8,3, respectively 1 serumtests verified. the anamnesis, diagnostics and therapy of the 3 women is reported. | 1977 | 562048 |
| non-specific urethritis. a placebo-controlled trial of minocycline in conjunction with laboratory investigations. | the results of a double-blind therapeutic trial on 81 men suffering from non-specific urethritis (nsu) show that minocycline was more effective than a placebo. before treatment chlamydia trachomatis was isolated from 31 per cent. of the men, ureaplasmas from 58 per cent., and mycoplasma hominis from 7-5 per cent. there is evidence that chlamydiae are a cause of urethritis. however, after minocycline therapy improvement in the clinical response of patients from whom only ureaplasmas were isolated ... | 1976 | 786440 |
| adaptation of mycoplasma hominis to an obligate parasitic existence in monkey kidney cell culture (bsc-1). | during attempts to eliminate mycoplasma hominis from a monkey kidney bsc-1 cell line with antibiotics, the mycoplasmas were isolated repeatedly. however, the organisms ultimately failed to grow on medium although electron microscopy confirmed that the cell culture still contained mycoplasmas. thus, the mycoplasma had adapted to an environment in which viable cells were required for growth. budding mycoplasmas which are indicative of replication were seen associated with viable cells extracellula ... | 1975 | 807916 |
| binding of lectins to membranes of mycoplasmas from aging cultures. | the binding of iodinated concanavalin a (con a) and ricinus communis agglutinin (rca) to intact cells and isolated membranes of acholeplasma laidlawii, mycoplasma hominis and mycoplasma capricolum decreased with the progression of the culture from the mid- to the late-logarithmic phase of growth. the binding of the lectins to acholeplasma laidlawii membranes had no significant effect on membrane fluidity, as assessed by electron-paramagnetic resonance spectroscopy of spin-labelled fatty acids, a ... | 1979 | 542135 |
| diagnosis of mycoplasma hominis pyelonephritis by demonstration of antibodies in urine. | to evaluate the diagnostic significance of the demonstration in urine of antibodies to mycoplasma hominis, 1,000 samples of urine with more than 5 leukocytes per high-power field were serologically investigated by indirect hemagglutination, using glutaraldehyde-fixed erythrocytes coated with m. hominis antigen. the samples were collected from 702 patients. antibodies were demonstrated in the urine of nine patients, all of whom had signs of acute attack of pyelonephritis. in seven of these patien ... | 1979 | 500801 |
| effect of metacycline treatment on non-acute prostatitis. | sixty-nine patients with symptoms of non-acute prostatitis were treated with metacycline and placebo according to the double-blind crossover technique. the patients reported improvement significantly more often after metacycline than after placebo treatment. but no difference was found between metacycline and placebo in respect of the palpatory findings or the number, morphology and motility of spermatozoa, the number of white blood cells in expressed prostatitic fluid, the zinc, magnesium or fr ... | 1975 | 813297 |
| neonatal central nervous system infection due to mycoplasma hominis. | | 1977 | 839380 |
| t-strain mycoplasma in the chimpanzee. | specimens from 4 chimpanzees were cultured for t-strain mycoplasma and mycoplasma hominis. t-strain mycoplasmas were recovered from the genital tract and throat of a male and the genital tract of his female cagemate; neither had clinical evidence of infection. two other male chimpanzees were culturally negative for t-strain mycoplasmas. m hominis was not isolated from any of the animals. the chimpanzee may serve as a suitable experimental model for studying the role of t-strain mycoplasmas in hu ... | 1976 | 1263431 |
| experimental animal infections with mycoplasma hominis and ureaplasma urealyticum. | subcutaneous tissue cavities in mice and guinea pigs were infected with human isolates of ureaplasma urealyticum and mycoplasma hominis. the minimal infective dose for m. hominis was as low as less than 10 color-changing units (ccu) for mice and 10(2) ccu for guinea pigs. the minimal infective dose for u. urealyticum was as low as less than 10 ccu for mice and 10(4) ccu for guinea pigs. mouse infections with either u. urealyticum or m. hominis persisted for 1 day to greater than 4 months. guinea ... | 1977 | 873611 |
| [mycoplasma infections of urogenital tract]. | our investigationes during the last seven years stress the importance of mycoplasma hominis and ureaplasma on the so called abacterial inflammation of the urogenital tract. because of diagnostic problems and differential diagnoses only specialized centers should explore these inflammatory conditions. therapy is possible, however, by dermatologists, urologists, gynecologists, and general practitioners. | 1977 | 885718 |
| microbiological investigation of bartholin's gland abscesses and cysts. | percutaneous aspirates from intact bartholin's gland cysts (12) and abscesses (34) were examined for bacteria and genital mycoplasmas. aspirates from 10 of the 12 patients with bartholin's gland cysts were sterile. aspirates from the other two cysts contained organisms that are part of the vaginal microbial flora. of the 34 abscesses, 24 (70.6 per cent) contained bacteria. eight abscesses contained gram-negative rods in pure culture and four contained gonococci in pure culture. twelve abscesses ... | 1977 | 900177 |
| cholesterol-phosphatidylcholine dispersions as donors of cholesterol to mycoplasma membranes. | growing cells of sterol-requiring mycoplasma hominis and sterol non-requiring acholeplasma laidlawii were used to test the ability of cholesterol-dipalmitoyl phosphatidylcholine dispersions to serve as cholesterol donors to these organisms. dispersions with high cholesterol to phosphatidylcholine ratios were more effective than dispersions with low cholesterol to phosphatidylcholine ratios in donating cholesterol to the membranes of both mycoplasmas and in promoting growth of the sterol-requirin ... | 1977 | 921974 |
| bacteriology of the urethra in normal men and men with nongonococcal urethritis. | sixty-nine caucasian males without a previous history of urethritis and who developed nongonococcal urethritis (ngu) and 39 similar men without urethritis (nu) were cultured from the urethra for chlamydia trachomatis, mycoplasma hominis, ureaplasma urealyticum, aerobes, and anaerobes. c. trachomatis infection was proven by culture of serology in 26 (38%) of the ngu group and 1 (3%) of the nu group; the c. trachomatis-negative ngu group had significantly more u. urealyticum (81%) than the c. trac ... | 1977 | 925148 |
| [morphology and membrane structures of mycoplasma hominis and t-mycoplasma]. | | 1976 | 969885 |
| [epidemiological study on mycoplasmas colonization and infection in the female genital tract]. | 1309 cervical or vaginal specimens from females in various physiological periods and women with multiple sexual partners were examined for ureaplasma urealyticum and mycoplasma hominis from feb. to may, 1990. significantly, low carriage rates of u.urealyticum were found in the neonatal (28.57%), puerperium (26.51%) and postmenopause (30.00%) groups, whereas, pregnant women (more than 36 weeks) showed the highest incidence (73.20%). the recovery rate of u. urealyticum in girls with no history of ... | 1992 | 1303321 |
| [immunologic structure of the population of several regions to mycoplasma hominis]. | | 1976 | 1015074 |
| studies on the new cell line tt-i: viral susceptibility and chromosomal changes related to mycoplasma contamination. | for understanding some biological properties of tt-1 cell line established in our laboratory in 1962, its viral susceptibility and the changes in chromosomes related to mycoplasma contamination were studied. tt-1 cells were susceptible to the infection of picorna (polio type 1, coxsackie b1 and echo type 2), measles, newcastle disease, herpes simplex (type 1), adeno (type 12) and vaccinia viruses. contamination of tt-1 cells with mycoplasma hominis was treated successfully with bottromycin a2 (5 ... | 1976 | 1037533 |
| vaginal microbial flora as a cofactor in the pathogenesis of uterine cervical intraepithelial neoplasia. | the vaginal microbial flora of 106 women with histopathologically confirmed cervical intraepithelial neoplasia and 79 women without disease, was evaluated for gardnerella vaginalis, trichomonas vaginalis, candida albicans and other yeasts. flora morphology was assessed by gram staining of secretions. cervical cultures were examined for herpes simplex virus, cytomegalovirus and neisseria gonorrhoeae. chlamydia trachomatis antigens in cervical secretions were detected by enzyme immunoassay. human ... | 1992 | 1351005 |
| genital mycoplasmal infections: their relation to prematurity and other abnormalities of reproduction. | the genital mycoplasmas, mycoplasma hominis and ureaplasma urealyticum et-mycoplasmas) are common vaginal organisms. they are acquired primarily through sexual contact. there is evidence, some of it highly suggestive, linking the genital mycoplasmas to involuntary infertility, spontaneous abortion and low birth weight. additional controlled treatment studies are needed in each of these areas to assess fully the role of the genital mycoplasmas. m. hominis has the potential to invade the blood str ... | 1976 | 1067274 |
| [further investigations for the demonstration of a specific microorganism in sympathetik ophthalmia]. | 19 following the inoculation of tissue culture cells with material obtained from 3 human eyes suffering from sympathetic ophthalmia (2 sympathizing = sy-l, 1 sympathized = sy-2), no growth of bacteria was observed. on the other hand, changes occurred such as are typical for a massive infection with mycoplasmas. the morphology of these microbes corresponds, down to the details, with micro-organisms which the author has frequently demonstrated and illustrated since 1948 in human, chicken and monke ... | 1975 | 1091180 |
| combined type-specific antisera in the identification of mycoplasma hominis. | in the usual serologic test for mycoplasma hominis, disks containing antibody to a prototype strain are applied to an agar plate that has been inoculated with the microorganism to be tested, and inhibition of growth is studied. the recent demonstration that m. hominis is not serologically homogeneous but consists of at least seven serotypes suggested the need for reexamination of the specificity of the disk test. the inhibition of growth of m. hominis on solid medium by disks containing seven an ... | 1975 | 1094075 |
| microbial flora of the lower genital tract during pregnancy: relationship to morbidity. | nineteen genera and groups of micro-organisms were isolated from the lower genital tract of 280 women at their first antenatal visit. chlamydia, viruses, and t-strain mycoplasmas were not sought, and only routine methods of anaerobic culture were used. growth was recorded as scanty, moderate or heavy. the population studied was grouped according to age, parity, gestational stage at booking, presence and degree of severity of lower genital tract morbidity, past history of vulvovaginitis, and susp ... | 1975 | 1100681 |
| mycoplasma hominis: growth, reproduction, and isolation of small viable cells. | improved methods for studying the growth of mycoplasma hominis (atcc 14027) have been developed, involving modified growth conditions and preparation of the organisms under minimally distorting conditions. cells so prepared from batch cultures show relatively uniform exponential growth and appear to be dividing by binary fission; but pleomorphic forms appear upon further incubation. similar behavior was demonstrated by another laboratory-adapted strain and by three clinical isolates, and therefo ... | 1975 | 1102522 |
| fatal intrauterine infection associated with mycoplasma hominis. | | 1992 | 1420708 |
| mycoplasma meningitis in infants with spina bifida. | infants with myelomeningocele are liable to develop bacterial colonisation of the lesion which, in the case of gram-negative bacteria, often progresses to meningitis because of the infants' immature immune status. the time and origin of the bacterial colonisation usually are uncertain. this study reports seven cases of meningitis caused by mycoplasma hominis, an organism not previously reported to cause meningitis. the commensal status of mycoplasma hominis in the vagina of many women, and its i ... | 1975 | 1107109 |
| immunological studies on the localization of phosphatidylglycerol in the membranes of mycoplasma hominis. | phosphatidylglycerol is the main component (87%) of the membrane phospholipids of mycoplasma hominis. it is immunologically active. antibodies directed against phosphatidylglycerol were detected in rabbits intravenously immunised with native m. hominis or isolated m. hominis membranes. the intravenous method of immunisation was chosen in order to select for a response to surface antigenic determinants. anti-phosphatidylglycerol antibodies were induced in rabbits by intravenously injecting the fl ... | 1975 | 1158331 |
| chorioamnionitis and colonization of the newborn infant with genital mycoplasmas. | to study the role of mycoplasma hominis and t-mycoplasmas (ureaplasma urealyticum) in chorioamnionitis, we obtained culture from 249 puerperal women and their babies. the placentas were examined histologically. infants whose placentas showed inflammation (chorioamnionitis) had cultures positive for t-mycoplasmas more frequently (37.5 per cent) than those with normal placentas (19.0 per cent) (p = 0.021). colonization with m. hominis was found in 16.0 per cent of the babies and was not significan ... | 1975 | 1168854 |
| prevalence of mycoplasma hominis and ureaplasma urealyticum (t strains) in urine of adolescents. | adolescent children were surveyed for colonization with mycoplasma hominis and ureaplasma urealyticum by culturing urine specimens. rates were compared between three study groups: (i) 397 children attending parochial schools, (ii) 293 children attending an adolescent clinic specializing in adjustment problems, and (iii) 86 children attending a renal clinic. the recovery rate was higher among postpubertal girls attending the renal clinic (33%) and the adolescent clinic (26%) than among students a ... | 1975 | 1176630 |
| failure to show mycoplasmas and cytopathogenic virus in rheumatoid arthritis. | synovial needle biopsies, joint aspirates, and joint tissue obtained at open operation from 41 cases of rheumatoid arthritis were inoculated onto pplo media, l-form medium, and cell cultures for the isolation of mycoplasmas, l-form bacteria, and viruses. medium suitable for the isolation of 't' strain mycoplasmas was not employed. no mycoplasmas, l-form bacteria, or cytopathogenic viruses were shown. similar specimens from nine patients diagnosed as having reiter's disease were examined in a lik ... | 1975 | 1190856 |
| the anti-mycoplasmal activity of aminosidine. | some in vivo experiments of therapeutic activity in severe experimental infection (intracranial) in the mouse due to different strains of mycoplasma proved the high efficacy of the antibiotic aminosidine when administered subcutaneously at the dose of 30 mg/kg and have also demonstrated that oral treatment is moderately effective against mycoplasma hominis 2. sensitivity test carried out in vitro on the same mycoplasma strains confirmed the bactericidal activity of aminosidine. these experiments ... | 1975 | 1204831 |
| characterization of the mycoplasma membrane proteins. vi. composition and disposition of proteins in membranes from aging mycoplasma hominis cultures. | membranes of mycoplasma hominis cells from cultures progressing from the mid to the end of the logarithmic phase of growth became richer in protein, poorer in phospholipids and cholesterol, heavier in density, and more viscous as determined by epr. the membrane-bound atpase activity declined steeply. electrophoretic analysis failed to show marked changes in membrane protein composition on aging, apart from an increase in the staining intensity of one protein band (mr approximately 130 000) conco ... | 1976 | 1252508 |
| role of arginine deiminase in growth of mycoplasma hominis. | arginine has been considered as the major energy source of nonglycolytic arginine-utilizing mycoplasmata. when three strains of mycoplasma arginini, and one strain each of mycoplasma arthritidis, mycoplasma fermentans, mycoplasma gallinarum, mycoplasma gallisepticum and mycoplasma hominis were grown in the medium with high arginine concentration (34 mm) compared with low arginine (4 mm), both the protein content of the organisms and the specific activity of arginine deiminase increased. m. ferme ... | 1976 | 1262306 |
| serological studies on mycoplasma hominis: serotypic distribution of recent clinical isolates. | serotyping of strains of mycoplasma hominis, freshly isolated from various sources, was conducted using the complement-independent growth agglutination (ga) test, using the 7 reference antisera prepared in our laboratory. multiple isolates from a common source indicated that only a single serotype tends to be found, making it possible to serotype clinical isolates without further cloning. however, cultures collected at different times from the same individual may contain different serotypes. a t ... | 1976 | 1265446 |
| rapid detection of tetm in mycoplasma hominis and ureaplasma urealyticum by pcr: tetm confers resistance to tetracycline but not necessarily to doxycycline. | tetracycline resistance in mycoplasma hominis and ureaplasma urealyticum has been associated with the tetm determinant and has recently been increasing in incidence. we report here a rapid method for detection of the tetm determinant based on the use of the polymerase chain reaction (pcr) to amplify a 397-bp dna fragment from the tetm gene and verification of specificity using the restriction enzyme taqi. analysis of 42 u. urealyticum and 49 m. hominis isolates indicates that the pcr method may ... | 1992 | 1526460 |
| lumbar pain caused by mycoplasma infection. | a 45-year-old woman was admitted to hospital following acute onset of lower back pain. clinical and laboratory investigations established a lumbar paraspinal soft tissue infection with mycoplasma hominis associated with severe spondylarthrosis at l5/s1. a relationship to a recently performed hysterectomy must be considered. | 1992 | 1533852 |
| [uses of vaginal ph measurement for prevention of premature labor. results of a prospective study]. | the influence of the microflora of the vagina and cervix has been undervalued for the induction of premature birth. examination of the microbiological problem by determination of the vaginal ph in combination with the microflora (chlamydia trachomatis, mycoplasma hominis, ureaplasma urealyticum). report of the outcome of 161 prospective, ad the beginning normal pregnancies in comparison of 200 primary hospitalized pregnancies with symptoms of imminent premature birth. conclusion: the vaginal ph ... | 1992 | 1290278 |
| [study on the infections of u. urealyticum, m. hominis, and c. trachomatis in patients with venereal diseases and healthy controls in three areas of china]. | two hundred and thiry-nine patients with venereal diseases and 264 healthy controls, from yichang, liuzhou, and beijing, were detected for the antibodies to ureaplasma urealyticum (uu). mycoplasma hominis (mh), and chlamydia trachomatis (ct) by indirect hemagglutination (iha) in 1990. the results showed that: 1) in patients with venereal diseases, the positive rates was the highest in ct (27.62%), higher in uu (17.57%), and lower in mh (4.18%); 2) the levels of antibody to ct and uu were signifi ... | 1992 | 1301266 |
| [research on enzymes of mycoplasma: enolase of mycoplasma hominis]. | a screening of enzymes on cell-free extracts of various species of mycoplasmas revealed the presence of enolase (ec 4.2.1.11) in significative amount in m. pneumoniae and m. fermentans, in lower amounts in m. hominis, a. laidlawii and in trace only in u. urealyticum. the value of activity of the various mycoplasmas could be correlated with their metabolism. from 40 g of cell paste of m. hominis, 2.5 mg of enolase purified over 70 folds, was obtained with successive steps of salt fractionation an ... | 1976 | 1023880 |
| mycoplasmemia in adult male patients. | mycoplasma hominis mycoplasmemia was associated with obstruction, manipulation, or surgery of the genitourinary tract in five adult men. a four-fold increase in specific antibody titer against m hominis was demonstrated in serial convalescent serum obtained from one patient. two patients became afebrile after treatment with tetracycline; a third recovered without therapy for a mycoplasm infection. the two remaining patients died of thier underlying diseases. | 1976 | 1036517 |
| lack of activity of zidovudine against ureaplasma urealyticum and mycoplasma hominis. | | 1992 | 1354810 |
| decreased metabolism and viability of mycoplasma hominis induced by monoclonal antibody-mediated agglutination. | monoclonal antibodies (mabs) were generated against lysates of clinical mycoplasma hominis isolates. three of these, designated bg2, ba10, and fe6, recognized an integral membrane protein of m. hominis with an apparent molecular weight of 50,000 (p50). electron microscopy studies demonstrated that this protein is distributed evenly over the cell surface. these anti-p50 mabs were species specific for m. hominis; they reacted with 42% of 126 tested clinical m. hominis isolates and showed no reacti ... | 1992 | 1370272 |
| [epidemiological factors and vaginal flora changes in vaginal bacteriosis (bacterial vaginosis)]. | the aim of this study was to know the modifications of the vaginal bacterial flora that occurs in bacterial vaginosis and to know the involvement of these microorganisms and the influence of several epidemiologic factors in the etiology of this disease. | 1992 | 1391016 |
| [ureaplasma urealyticum and mycoplasma hominis: incidence and clinical significance of their isolation in the perinatal period]. | in order to known the clinical significance of the colonization of the maternal genitalia and infant with genital mycoplasmas, we studied prospectively 219 pairs of mothers and newborns admitted for delivery to a general public hospital. u. urealyticum and m. hominis were isolated from 72 (32.9%) and 10 (4.6%) of the mothers, respectively. during the first 4 days of life. u. urealyticum and m. hominis were isolated from 23 (10.5%) and 1 (0.5%), of the newborn infants respectively. maternal colon ... | 1992 | 1605412 |
| [epidemic investigation of genital mycoplasma hominis infection in women]. | the genital mycoplasma hominis infection in women was investigated at xiaguan district, nanjin in june, 1990. leucorrheas from 722 women were tested with elisa for the antigen of m. hominis. the prevalences of genital m. hominis were not statistically different among age and occupation groups. women having pregnancy more than three times had a significantly higher infection rate, it is suggested that induced abortion might provide chances of the infection. the prevalence was significantly differ ... | 1992 | 1394388 |
| [significant bacteriospermia. value and limits of sperm count in andrology]. | the data concerning bacteriospermia--are very different in the literature. asymptomatic andrologic patients, and as control group--patients of the outpatient department for male adnexitis--were studied. cultivation of aerobic bacteria and mycoplasmas has been recently accomplished by quantitative bacteriological analysis and chlamydia diagnostic. the microbiological screening of 210 andrologic patients ejaculate has given the following results: 19.6% were sterile, 21.0% were colonised by apathog ... | 1992 | 1408103 |
| microbial causative agents of male urethritis. | the incidence of chlamydia trachomatis, ureaplasma urealyticum, mycoplasma hominis, neisseria gonorrhoeae and trichomonas vaginalis was studied in men with urethritis. out of 150 examined men 48.7% had the positive isolation of u. urealyticum, 26.0% c. trachomatis, 22.7% n. gonorrhoeae, 18.7% m. hominis and in one (0.7%) patient t. vaginalis was found. none of the above mentioned microorganisms was detected in 24.7% of examined men. in 43.3% cases only one agent was isolated. in 23.3% of the men ... | 1992 | 1619287 |
| quinolones as an alternative treatment of chlamydial, mycoplasma and gonococcal urogenital infections. | we report the results of several trials aimed at evaluating the quinolones in urogenital infections. in chlamydia trachomatis infections, ofloxacin (200 mg b.i.d. for 10 days) gave a cure rate of 98% (n = 66), and fleroxacin (400 mg s.i.d. for 7 days) provided a cure rate of 89% (n = 19). a double-blind study comparing fleroxacin (600 mg s.i.d.) to doxycycline (100 mg b.i.d.) for 7 days showed similar high cure rates for both regimens (100%; n = 23). in mycoplasma hominis infections, ofloxacin ( ... | 1992 | 1421625 |
| in vitro activity of several antimicrobial agents against genital mycoplasmas. | the increasing resistance of genital mycoplasmas to tetracyclines is a serious problem, since this group of antibiotics is one of the few that is effective against virtually all species of mycoplasmas. tetracyclines are also used to treat many sexually transmitted diseases. in this study, we assessed the susceptibility of mycoplasma hominis and ureaplasma urealyticum to macrolides, tetracyclines, spectinomycin, and trospectomycin by the agar dilution method. for m hominis, trospectomycin was the ... | 1992 | 1468088 |