| uptake, transbilayer distribution, and movement of cholesterol in growing mycoplasma capricolum cells. | | 1978 | 83152 |
| mycoplasma phosphoenolpyruvate-dependent sugar phosphotransferase system: glucose-negative mutant and regulation of intracellular cyclic amp. | glucose-negative mutants of mycoplasma capricolum were selected for growth on fructose in the presence of the toxic glucose analog alpha-methyl-d-glucopyranoside. the mutants are defective in the phosphoenolpyruvate:sugar phosphotransferase system for glucose. one mutant, pts-4, was studied in detail. it lacks the glucose-specific, membrane-bound enzyme ii, iiglc, as well as the general, low-molecular-weight, phosphocarrier protein, hpr. in place of the latter, however, it has a fructose-specifi ... | 1978 | 201608 |
| sterol requirement of mycoplasma capricolum. | mycoplasmas require an external source of sterol for growth. for mycoplasma capricolum this requirement is met not only by cholesterol but also by the methylcholestane derivatives lanosterol, cycloartenol, 4,4-dimethylcholesterol, and 4beta-methylcholestanol. cholesteryl methyl ether and 3alpha-methylcholestanol serve equally well as sterol supplements. none of the growth-supporting sterol derivatives tested was metabolically modified. the unusual acceptance of diverse cholestane derivatives by ... | 1978 | 279900 |
| on the role of the sterol hydroxyl group in membranes. | the adequacy of sterol derivatives containing a blocked 3-hydroxyl group for sustaining the growth of two sterol auxotrophs has been investigated. mycoplasma capricolum, a cholesterol-requiring bacterium, grows nearly as well on media supplemented with cholesteryl methyl ether or cholesteryl acetate as on free cholesterol. the two derivatives are recovered unchanged from the bacterial cells. similarly, cholesteryl methyl ether or ergosteryl methyl ether replace cholesterol or ergosterol as stero ... | 1979 | 387751 |
| speculations on the evolution of sterol structure and function. | the essential oxygen requirement for sterol biosynthesis dates this molecule as a relative latecomer in cellular evolution. structural details of the cholesterol molecule and related sterols can be rationalized in terms of optimal hydrophobic interactions between the planar sterol ring system and phospholipid acyl chains in the membrane bilayer. the prediction that the cholesterol precursor lanosterol (4,4',14 trimethyl cholastadienol) is incompetent for membrane function is verified by in vivo ... | 1979 | 498798 |
| binding of lectins to membranes of mycoplasmas from aging cultures. | the binding of iodinated concanavalin a (con a) and ricinus communis agglutinin (rca) to intact cells and isolated membranes of acholeplasma laidlawii, mycoplasma hominis and mycoplasma capricolum decreased with the progression of the culture from the mid- to the late-logarithmic phase of growth. the binding of the lectins to acholeplasma laidlawii membranes had no significant effect on membrane fluidity, as assessed by electron-paramagnetic resonance spectroscopy of spin-labelled fatty acids, a ... | 1979 | 542135 |
| mycoplasma capricolum associated with arthritis in sheep. | | 1977 | 595298 |
| interaction between tetanolysin and mycoplasma cell membrane. | 1. a partially purified tetanolysin preparation lysed the sterol-requiring mycoplasma capricolum cells but had no effect on m. capricolum cells adapted to grow with no or very little cholesterol. the sterol-non-requiring acholeplasma laidlawii cells grown either in a cholesterol-rich or a cholesterol-poor medium were unaffected by the tetanolysin preparation. 2. the lysis of m. capricolum cells by the tetanolysin preparation was temperature dependent, inhibited by cholesterol, sublytic concentra ... | 1976 | 793633 |
| relationships between members of the mycoplasma mycoides cluster as shown by dna probes and sequence analysis. | a gene probe, cap-21, which demonstrated interrelationships between the members of the mycoplasma mycoides cluster was developed. the probe easily differentiated mycoplasmas in this cluster by clear and predictable hybridization patterns in southern blots and separated the cluster into four groups. strains of m. mycoides subsp. mycoides which were capable of causing contagious bovine pleuropneumonia composed one group. strains of m. mycoides subsp. mycoides which did not cause contagious bovine ... | 1992 | 1390110 |
| development of a species-specific dna probe for mycoplasma capricolum. | a specific dna probe for the detection and identification of mycoplasma capricolum, one of the causative agents of contagious agalactia syndrome, was selected from a genomic library. it consists of a 900bp rsai genomic fragment of m. capricolum (reference strain), cloned into the ecorv site of the plasmid bluescript. by using the appropriate stringency this radiolabelled probe reacts specifically with m. capricolum when tested by dot blot hybridization against various mycoplasmal dnas. the curre ... | 1992 | 1441199 |
| structure of the dnaa and dnaa-box region in the mycoplasma capricolum chromosome: conservation and variations in the course of evolution. | we have previously shown that the dnaa gene and the dnaa-box region were conserved in bacteria representative of all three major branches of the eubacterial phylogenic tree: high g + c gram+, low-g + c gram+ and gram-. in the present work, we determined the structure of the dnaa region of mycoplasma capricolum and found that the dnaa gene and at least two other genes, rpmh and dnan, were conserved in this bacterium. an unusually high level of amino acid (aa) substitutions was observed in m. capr ... | 1992 | 1544573 |
| evolutionary relationships of a plant-pathogenic mycoplasmalike organism and acholeplasma laidlawii deduced from two ribosomal protein gene sequences. | the families within the class mollicutes are distinguished by their morphologies, nutritional requirements, and abilities to metabolize certain compounds. biosystematic classification of the plant-pathogenic mycoplasmalike organisms (mlos) has been difficult because these organisms have not been cultured in vitro, and hence their nutritional requirements have not been determined nor have physiological characterizations been possible. to investigate the evolutionary relationship of the mlos to ot ... | 1992 | 1556079 |
| genetic analysis of an aphid endosymbiont dna fragment homologous to the rnpa-rpmh-dnaa-dnan-gyrb region of eubacteria. | buchnera aphidicola is a gram- eubacterium with a dna g+c content of 28-30 mol%. this organism is an obligate intracellular symbiont of aphids. to determine its similarity to or difference from other eubacteria, a 4.9-kb dna fragment from b. aphidicola containing the gene homologous to escherichia coli dnaa (a gene involved in the initiation of chromosome replication) was cloned into e. coli and sequenced. the order of genes on this fragment, 60k-10k-rnpa-rpmh-dnaa-dnan-gyrb, was similar to that ... | 1992 | 1572539 |
| a cluster of nine trna genes between ribosomal gene operons in bacillus subtilis. | a cluster of nine trna genes located in the 1-kb region between ribosomal operons rrnj and rrnw in bacillus subtilis has been cloned and sequenced. this cluster contains the genes for trna(uacval), trna(uguthr), trna(uuulys), trna(uagleu). trna(gccgly), trna(uaaleu), trna(acgarg), trna(uggpro), and trna(ugcala). the newly discovered trna gene cluster combines features of the 3'-end of trni, a cluster of 6 trna genes between ribosomal operons rrni and rrnh, and of the 5'-end of trnb, a cluster of ... | 1992 | 1577685 |
| the organization and evolution of transfer rna genes in mycoplasma capricolum. | the genes for presumably all the trna species in mycoplasma capricolum, a derivative of gram-positive eubacteria, have been cloned and sequenced. there are 30 genes encoding 29 trna species. this number is the smallest in all the known genetic systems except for mitochondria. the sequences of 9 trna genes of them have been previously reported (1-3). twenty-two genes are organized in 5 clusters consisting of nine, five, four and two genes (2 sets), respectively. the other eight genes exist as a s ... | 1990 | 1698277 |
| physical map of the chromosome of lactococcus lactis subsp. lactis dl11 and localization of six putative rrna operons. | a physical map of the chromosome of lactococcus lactis subsp. lactis dl11 was constructed by using the contour-clamped homogeneous electric field mode of pulsed-field gel electrophoresis in one- and two-dimensional separations to analyze restriction digests of high-molecular-weight genomic dna. the map, which shows all the observed noti and smai sites (six and 21, respectively) and 8 of approximately 30 sali sites, is circular and yields a total size of 2.58 megabase pairs for the l. lactis subs ... | 1991 | 1708377 |
| translation in vitro of codon uga as tryptophan in mycoplasma capricolum. | the in-frame uga codons in the synthetic messenger rna were translated in the cell-free system of mycoplasma capricolum. the result, together with the occurrence of codon uga at tryptophan sites in the genes and the presence of trna(ucatrp) pairing with uga, clearly indicated that uga is a tryptophan codon in this bacterium. | 1991 | 1720664 |
| [the interaction of alkylating derivatives of oligodeoxyribonucleotides and their methylphosphonate analogs with mycoplasma cells]. | alkylating derivatives of decathymidylates and methylphosphonate analogs of oligodeoxyribonucleotides (mpao) were studied for their interaction with cells of acholeplasma laidlawii pg-8, mycoplasma capricolum california kid, m. pneumoniae fh and phytopathogenic strain (st. 118). it is shown that mpao of octa- and hexadecathymidylates as well as decathymidylates 3'-terminal modified by phenazine and cholesterol groupings are sorbed by mycoplasma cells and can penetrate inside the cells. efficienc ... | 1991 | 1753887 |
| structure and function of dnaa and the dnaa-box in eubacteria: evolutionary relationships of bacterial replication origins. | dnaa protein (a trans-acting element) and its binding sequence, dnaa-box: (a cis-acting element) are two elements essential for the initiation of chromosomal replication in escherichia coli and other enteric bacteria. recently these two elements have been found to be conserved in three gram-positive bacteria (bacillus subtilis, micrococcus luteus and mycoplasma capricolum) as well as in gram-negative pseudomonads. dnaa protein was also found to be essential in the initiation of the replication o ... | 1991 | 1779750 |
| detection of antibodies to mycoplasmas in south american camelids. | indirect haemagglutination tests on sera from 757 south american camelids (alpacas, llamas and vicunas) carried out in the andean region of peru, revealed evidence of exposure mainly to mycoplasma mycoides subspecies mycoides lc. the incidence of detectable antibodies to this mycoplasma in 554 alpacas was 5.0 per cent and in 141 llamas 15.6 per cent. antibody to mycoplasma capricolum and the f38 biotype was detected in 0.9 per cent and 0.2 per cent of alpacas, respectively. in a group of 62 vicu ... | 1991 | 1780576 |
| a physical map for mycoplasma capricolum cal. kid with loci for all known trna species. | | 1991 | 1849633 |
| successive synovial mycoplasma hominis isolates exhibit apparent antigenic variation. | the expression of surface proteins by 14 successive mycoplasma hominis isolates obtained from the synovial fluid of a chronically infected septic arthritis patient was examined. marked differences in the expression of surface proteins, as determined by monoclonal antibodies raised against the first isolate, were observed. however, identical restriction patterns and virtually identical hybridization patterns with probes containing the conserved genes of the mycoplasma capricolum rrna operon and t ... | 1991 | 1879948 |
| small unilamellar vesicles are able to fuse with mycoplasma capricolum cells. | we have investigated the fusion characteristics of intact mycoplasma capricolum cells and small unilamellar vesicles (suv). the rate and extent of fusion was monitored continuously by octadecylrhodamine b (r18) fluorescence dequenching assay, as well as by intracellular contents mixing, and by sucrose density gradient analysis. the fusion of suv with m. capricolum cells was found to be dependent on poly(ethylene glycol) (peg 8000), divalent cations in the medium, and on the cholesterol content o ... | 1991 | 1901498 |
| ferredoxin and ribosomal protein s10 are encoded on the cyanelle genome of cyanophora paradoxa. | the petf and rsp10 genes of the cyanellar genome of the taxonomically ambiguous flagellate cyanophora paradoxa have been cloned, mapped, and sequenced. in higher plants these genes are not encoded in the chloroplast dna, but are encoded in the nucleus. the c. paradoxa petf gene predicts a protein of 99 amino acids (aa) which is more similar to type-i ferredoxins of diverse cyanobacteria than to those of green algae, dinoflagellates, and higher plants. the rsp10 gene (rspj) predicts a protein of ... | 1991 | 1901820 |
| nucleotide sequence of the secy gene from lactococcus lactis and identification of conserved regions by comparison of four secy proteins. | sec y is an integral membrane protein which participates in the translocation of proteins through the bacterial cell membrane. we have cloned the sec y gene of lactococcus lactis, and found its deduced protein sequence, 439 amino acids long, to be similar in length to the previously determined sec y proteins of escherichia coli, bacillus subtilis and mycoplasma capricolum. comparison of the l. lactis sec y to the 3 other sec y proteins revealed 90 conserved amino acid residues (21%). nearly half ... | 1991 | 1908794 |
| fusion of mycoplasmas: the formation of cell hybrids. | poly(ethylene glycol) (peg 8000) can induce cell-cell fusion of mycoplasma capricolum cells, and it can promote the formation of intergeneric hybrids of various mycoplasma, acholeplasma and spiroplasma species. the extent of fusion was quantitatively evaluated by following the dequenching of octadecylrhodamine fluorescent label incorporated into donor cell membranes after their incubation with recipient cells. the results of dequenching experiments were confirmed by electron microscopy, as well ... | 1991 | 1936938 |
| levels of trnas in bacterial cells as affected by amino acid usage in proteins. | transfer rnas of mycoplasma capricolum were separated by two-dimensional polyacrylamide gel electrophoresis, and the relative abundance of each of the 28 known trna species was measured. there existed a correlation between the relative amount of isoacceptor trnas and the frequency in choosing synonymous codons that could be translated by the isoacceptors. furthermore, it was observed that the total amount of trnas for a particular amino acid was paralleled by the composition of the amino acid in ... | 1991 | 1956771 |
| identification and characterization of a dictyostelium discoideum ribosomal protein gene. | we have identified a developmentally repressed large-subunit ribosomal protein gene of dictyostelium discoideum based on sequence similarity to other ribosomal proteins. protein rpl7 is homologous to large subunit ribosomal proteins from the rat and possibly to mycoplasma capricolum and escherichia coli, but is not similar to three sequenced ribosomal proteins in dictyostelium. the rpl7 gene is present at one copy per genome, as are six other cloned dictyostelium ribosomal proteins. restriction ... | 1990 | 1975664 |
| cgg: an unassigned or nonsense codon in mycoplasma capricolum. | cgg is an arginine codon in the universal genetic code. we previously reported that in mycoplasma capricolum, a relative of gram-positive eubacteria, codon cgg did not appear in coding frames, including termination sites, and trna(argccg) pairing with codon cgg, was not detected. these facts suggest that cgg is a nonsense (unassigned and untranslatable) codon--i.e., not assigned to arginine or to any other amino acid. we have investigated whether cgg is really an unassigned codon by using a cell ... | 1991 | 1992485 |
| effect of cholesterol and lanosterol on the structure and dynamics of the cell membrane of mycoplasma capricolum. deuterium nuclear magnetic resonance study. | deuterium nuclear magnetic resonance (nmr) techniques were employed to study the effect of sterols on the composition and dynamics of the membrane lipids of mycoplasma capricolum, a natural fatty acid auxotroph that requires sterols for growth. the membrane lipids of cells grown in modified edwards medium supplemented with cholesterol, oleic acid (oa), and palmitic acid (pa) were composed primarily of phosphatidylglycerol (pg) (60%) and cardiolipin (cl) (35%). the incorporation of cholesterol an ... | 1991 | 2049526 |
| physical mapping of the mycoplasma capricolum genome. | a physical map of mycoplasma capricolum atcc 27343 genome was constructed, based on estimation of the restriction fragment sizes by pulse-field electrophoresis. the linkage order of restriction fragments was determined by two-dimensional electrophoresis of partial and complete single digests and complete double digests and by southern hybridization analysis. the genome size was established at 1155.5 kb, and 26 cleavage sites for 7 endonucleases were assigned to the map. | 1991 | 2060765 |
| evidence that uga is read as a tryptophan codon rather than as a stop codon by mycoplasma pneumoniae, mycoplasma genitalium, and mycoplasma gallisepticum. | molecular cloning and sequencing showed that mycoplasma gallisepticum, like mycoplasma capricolum, contains both trna(uca) and trna(cca) genes, while mycoplasma pneumoniae and mycoplasma genitalium each appear to have only a trna(uca) gene. therefore, these mycoplasma species contain a trna with the anticodon uca that can translate both uga and ugg codons. | 1990 | 2104612 |
| [in vitro antibiosensitivity of different strains of mycoplasma capricolum]. | the in vitro sensitivity of 20 wild strains of m. capricolum and that of the reference strain (california kid) against 14 antibiotics was investigated by means of a micromethod. the technique is based on the determination of the inhibitory minimum concentration (imc) in a liquid medium as revealed by the inhibition of glucose metabolism. the following results were obtained: all strains were sensitive to five antibiotics (tylosin, oxytetracyclin, gentamycin, neomycin, nalidixic acid) with an imc ... | 1990 | 2132784 |
| dissection of the 16s rrna binding site for ribosomal protein s4. | the ribosomal protein s4 from escherichia coli is essential for initiation of assembly of 30s ribosomal subunits. we have undertaken the identification of specific features required in the 16s rrna for s4 recognition by synthesizing mutants bearing deletions within a 460 nucleotide region which contains the minimum s4 binding site. we made a set of large nested deletions in a subdomain of the molecule, as well as individual deletions of nine hairpins, and used a nitrocellulose filter binding ass ... | 1990 | 2207164 |
| differences in accumulation of radiolabeled amino acids and polyamines by mycoplasma and acholeplasma species. | four species in the order mycoplasmatales, mycoplasma capricolum, mycoplasma hominis, mycoplasma arginini, and acholeplasma laidlawii, were compared for their ability to accumulate radiolabeled amino acids and polyamines. the use of a novel high-molecular-weight (hmw) medium, from which molecules of less than 12,000 molecular weight had been removed by extensive dialysis, allowed us to discern significant differences among the species in their relative accumulations of [3h]methionine and [3h]leu ... | 1990 | 2275861 |
| heat shock response in mycoplasmas, genome-limited organisms. | we have measured the effect of heat shock on three mycoplasmas (acholeplasma laidlawii k2 and ja1 and mycoplasma capricolum kid) and demonstrated the induction of mycoplasma heat shock proteins under these conditions. increased synthesis of at least 5 heat shock proteins in a. laidlawii k2, 11 heat shock proteins in a. laidlawii ja1, and 7 heat shock proteins in m. capricolum was observed by electrophoretic analysis of proteins from heat-shocked cells in sodium dodecyl sulfate-polyacrylamide gel ... | 1990 | 2318804 |
| mycoplasma capricolum membranes induce tumor necrosis factor alpha by a mechanism different from that of lipopolysaccharide. | heat-inactivated (60 degrees c, 45 min) mycoplasma capricolum strain jr cells activate murine macrophages to secrete high levels of tumor necrosis factor alpha (tnf alpha) and to lyse tumor target cells efficiently. fractionation of the intact m. capricolum cells, obtained from cells harvested at the exponential phase of growth, shows that their capacity to induce tnf alpha secretion by macrophage resides exclusively in the membrane fraction. the macrophage-mediated cytolysis following activatio ... | 1990 | 2322937 |
| the primary structure of rat ribosomal protein s20. | the amino acid sequence of the rat 40 s ribosomal subunit protein s20 was deduced from the sequence of nucleotides in two recombinant cdnas. ribosomal protein s20 has 119 amino acids and has a molecular weight of 13,364. hybridization of the cdna to digests of nuclear dna suggests that there are 16-19 copies of the s20 gene. the mrna for the protein is about 600 nucleotides in length. rat s20 is homologous to xenopus laevis s22 and is related to mycoplasma capricolum s10 and to escherichia coli ... | 1990 | 2357470 |
| increased rates of lipid exchange between mycoplasma capricolum membranes and vesicles in relation to the propensity of forming nonbilayer lipid structures. | we have studied the effects of modification of the endogenous phosphatidylglycerol (pg) and diphosphatidylglycerol (dpg) content of the plasma membrane of mycoplasma capricolum on the kinetics of spontaneous [14c]cholesterol and 14c-labeled phospholipid exchange between m. capricolum membranes and lipid vesicles. the pg/dpg molar ratio of m. capricolum membranes changed when cells were grown in media supplemented with 0.5 mm cacl2 and/or egg phosphatidylcholine (pc) (10-20 micrograms/ml), increa ... | 1990 | 2394716 |
| the genome structure of mycoplasma capricolum. | | 1987 | 2444556 |
| gene expression signals in mycoplasma hyopneumoniae and mycoplasma capricolum. | in mycoplasma hyopneumoniae, the single genes for 16s and 23s rrnas are clustered in one operon from which the 5s rrna gene is separated by more than 4 kbp. this operon, gene 2413 and gene x from m. hyopneumoniae and the 5' ends of both rrna operons from m. capricolum were cloned and used for analysis of the following gene expression signals: promoters and terminators of the dna-directed rna polymerase, ribosomal binding sites of mrna, and sites for processing of precursor rrna. the analyses wer ... | 1987 | 2444558 |
| directional mutation pressure and transfer rna in choice of the third nucleotide of synonymous two-codon sets. | bacterial species have diverged into a series of families, some with high g + c content in their dna, and other with high a + t content, resulting, respectively, from g.c- and a.t-directional mutation pressures. such mutation pressure (g.c/a.t pressure) may be an important determinant for codon usage. it has also been suggested that trna acts as a selective constraint for determining codon usage. we have studied the relation between g.c/a.t pressure and trna constraints in determining choice of ... | 1988 | 2448791 |
| codon recognition patterns as deduced from sequences of the complete set of transfer rna species in mycoplasma capricolum. resemblance to mitochondria. | the nucleotide sequences of the complete set of trna species in mycoplasma capricolum, a derivative of gram-positive eubacteria, have been determined. this bacterium represents the first genetic system in which the sequences of all the trna species have been determined at the rna level. there are 29 trna species: three for leu, two each for arg, ile, lys, met, ser, thr and trp, and one each for the other 12 amino acids as judged from aminoacylation and the anticodon nucleotide sequences. the num ... | 1989 | 2478713 |
| structural comparison of 26s rrna-binding ribosomal protein l25 from two different yeast strains and the equivalent proteins from three eubacteria and two chloroplasts. | the sequences of saccharomyces carlsbergensis ribosomal protein (r-protein) sl25* and its equivalents from candida utilis (cl25), escherichia coli (el23), bacillus stearothermophilus (bl23), mycoplasma capricolum (ml23), marchantia polymorpha chloroplasts (mcpl23), and nicotiana tabacum chloroplasts (ncpl23) were examined using a computer program that evaluates the extent of sequence similarity by calculating correlation coefficients for each pair of residues in two proteins from a number of phy ... | 1989 | 2501503 |
| novel arrangement of rrna genes in mycoplasma gallisepticum: separation of the 16s gene of one set from the 23s and 5s genes. | large restriction fragments from the dna of mycoplasma gallisepticum s6 and pg31, which were prepared by digestion with bgli, bsshii, smai, or xhoi and which were separated by pulsed-field electrophoresis, were hybridized with probes containing most, or different parts, of an rrna operon of mycoplasma capricolum. the results showed that the genomes contained three widely separated rrna loci. one locus contained genes for all three rrna species and another contained 23s and probably 5s rrna genes ... | 1989 | 2708324 |
| determination of microbial genome sizes by two-dimensional denaturing gradient gel electrophoresis. | in two-dimensional denaturing gradient gel electrophoresis, dna is digested with a restriction endonuclease and the resulting dna fragments are separated as a function of size by conventional agarose gel electrophoresis. following this first dimension electrophoresis, the fragment distribution is placed at the top of a denaturing gradient slab gel and electrophoresis is carried out parallel to the gradient direction. this second dimension separation is a complex function of the base sequence of ... | 1989 | 2786189 |
| 16s rrna sequence indicates that plant-pathogenic mycoplasmalike organisms are evolutionarily distinct from animal mycoplasmas. | the plant-pathogenic mycoplasmalike organisms (mlos) are so named because they lack cell walls. many features that are essential to a definitive classification remain uncharacterized, because these organisms have resisted attempts at in vitro culturing. to establish the taxonomic position of the mlos, the dna region containing the 16s rrna gene from a representative of the mlos has been cloned and sequenced. sequence comparisons indicate that the mlos are related to mycoplasma capricolum and tha ... | 1989 | 2808301 |
| spiroplasma virus 4: nucleotide sequence of the viral dna, regulatory signals, and proposed genome organization. | the replicative form (rf) of spiroplasma virus 4 (spv4) has been cloned in escherichia coli, and the cloned rf has been shown to be infectious by transfection (m. c. pascarel-devilder, j. renaudin, and j.-m. bové, virology 151:390-393, 1986). the cloned spv4 rf was randomly subcloned and was fully sequenced by the dideoxy chain termination technique, using the m13 cloning and sequencing system. the nucleotide sequence of the spv4 genome contains 4,421 nucleotides with a g+c content of 32 mol%. t ... | 1987 | 2822658 |
| animal viruses are able to fuse with prokaryotic cells. fusion between sendai or influenza virions and mycoplasma. | sendai and influenza virions are able to fuse with mycoplasmata. virus-mycoplasma fusion was demonstrated by the use of fluorescently labeled intact virions and fluorescence dequenching, as well as by electron microscopy. a high degree of fusion was observed upon incubation of both virions with mycoplasma gallisepticum or mycoplasma capricolum. significantly less virus-cell fusion was observed with acholeplasma laidlawii, whose membrane contains relatively low amounts of cholesterol. the require ... | 1988 | 2826447 |
| uptake of fatty acids by mycoplasma capricolum. | the energy requirements for fatty acid uptake by mycoplasma capricolum were studied. fatty acid transport and esterification to phospholipid appeared to be tightly coupled, since there was little intracellular accumulation of free fatty acid. uptake was blocked by iodoacetate, n-ethylmaleimide, and p-chloromercuribenzoate. glucose, glycerol, and potassium ions were necessary for fatty acid uptake by whole cells. a reduction in uptake was observed in cells treated with valinomycin or dicyclohexyl ... | 1988 | 2834319 |
| synthesis of deoxyribomononucleotides in mollicutes: dependence on deoxyribose-1-phosphate and ppi. | cell extracts of acholeplasma laidlawii b-pg9, acholeplasma morum s2, mycoplasma capricolum 14, and mycoplasma gallisepticum s6 were examined for 37 cytoplasmic enzyme activities involved in the salvage and biosynthesis of purines. all of these organisms had adenine phosphoribosyltransferase activity (ec 2.4.2.7) and hypoxanthine phosphoribosyltransferase activity (ec 2.4.2.8). all of these organisms had purine-nucleoside phosphorylase activity (ec 2.4.2.1) in the synthetic direction using ribos ... | 1987 | 3038846 |
| presence of anaplerotic reactions and transamination, and the absence of the tricarboxylic acid cycle in mollicutes. | cell extracts of the fermentative mollicutes acholeplasma laidlawii b-pg9, acholeplasma morum s2, mycoplasma capricolum 14, mycoplasma gallisepticum s6, mycoplasma pneumoniae fh, mycoplasma hyopneumoniae j and m. genitalium g-37, and the non-fermentative mycoplasma hominis pg-21, mycoplasma hominis 1620 and mycoplasma bovigenitalium pg-11 were examined for 39 cytoplasmic enzyme activities associated with the tricarboxylic acid (tca) cycle, transamination, anaplerotic reactions and other enzyme a ... | 1988 | 3141576 |
| [isolation of mycoplasma capricolum from bull semen]. | | 1988 | 3217561 |
| [comparison of the pathogenicity of 3 mycoplasma capricolum strains in adult and newborn goats]. | | 1988 | 3253856 |
| mycoplasma capricolum in an outbreak of polyarthritis and pneumonia in goats. | | 1988 | 3256232 |
| taxonomy of the mycoplasma mycoides cluster. | some of the mycoplasmas found in diseases of ruminants, mycoplasma capricolum, m. mycoides subsp. capri (pg3), bovine group 7 (pg50), strain f38 and strain y goat show various degrees of relationship to m. mycoides subsp. mycoides (pg1) and to one another, such that they are referred to as the m. mycoides cluster. studies using serology, dna homology, isoenzyme analysis and two-dimensional polyacrylamide gel electrophoresis (2-d page) of proteins have been carried out on the type and reference s ... | 1987 | 3312102 |
| the genome of mycoplasma capricolum. | | 1987 | 3326041 |
| promoters of mycoplasma capricolum ribosomal rna operons: identical activities but different regulation in homologous and heterologous cells. | the 5' region of the rrna operon, rrna, of m. capricolum was cloned. sequence analysis revealed two trna genes, trna(leu) and trna(lys), upstream to the promoter of the rrna operon. the in vivo transcription start sites of the rrna operon and of the trna genes were mapped. the same promoters used by m. capricolum rna polymerase are also recognized by e. coli rna polymerase both in vivo and in vitro. we find that high levels of ppgpp in e. coli, resulting from amino acid starvation or from spot m ... | 1988 | 3340543 |
| effect of 7 beta-hydroxycholesterol on growth and membrane composition of mycoplasma capricolum. | 7 beta-oh cholesterol in a cholesterol rich growth medium (5-10 micrograms/ml) extended the lag period and slowed down the growth rate of mycoplasma capricolum cells. in a cholesterol poor medium (0.5 micrograms/ml) inadequate to support growth, 7 beta-oh cholesterol exerts a synergistic effect on growth. the 7 beta-oh cholesterol was incorporated unchanged from the growth medium and could be recovered exclusively in the membrane fraction. the incorporation of the 7 beta-oh cholesterol has no ef ... | 1988 | 3378626 |
| evolutionary dynamics of tryptophan trnas in mycoplasma capricolum. | mycoplasma capricolum uses two tryptophan codons, the "universal" nonsense codon uga and the universal codon ugg. the bacterium contains two tryptophan trnas, one with anticodon uca, (u: 2'-o-methyl u derivative), and the other with cca (5'-c: partially 2'-o-methylated). trnauca would translate codons uga and probably ugg by wobbling. trnacca is much less charged by tryptophan in the cells than trnauca, and the intracellular amount of trnacca is 5-10 times lower than that of trnauca. the genes f ... | 1988 | 3405203 |
| analysis of transcription and processing signals in the 5' regions of the two mycoplasma capricolum rrna operons. | the transcription and rna processing signals of the rrna operons (rrna and rrnb) of mycoplasma capricolum were analyzed by mapping the 5' ends of in vivo and in vitro synthesized rnas. the results of both in vitro and in vivo analyses point to the rrna operon being transcribed from two promoters (p1 and p2) into large precursor rnas. transcripts initiating at p1 contain two trnas, and probably 16 s, 23 s, and 5 s rrnas, whereas the transcripts starting from p2 consist only of the three rrnas. th ... | 1988 | 3419421 |
| effects of intrauterine infection by staphylococcus aureus and mycoplasma capricolum on the fertility of nubian goats. | oestrous cycles were followed by blood progesterone titration and the occurrence of pregnancy was observed after mating in three groups of mature female nubian goats. two groups of 4 and 3 goats each were experimentally infected by an in utero injection of either a staphylococcus aureus or a mycoplasma capricolum strain of caprine origin. a third group of 4 goats served as a control. both bacterial infections persisted in all experimental animals, except one, for at least 70 days. as a result, t ... | 1987 | 3448724 |
| the ribosomal protein gene cluster of mycoplasma capricolum. | the dna sequence of the part of the mycoplasma capricolum genome that contains the genes for 20 ribosomal proteins and two other proteins has been determined. the organization of the gene cluster is essentially the same as that in the s10 and spc operons of escherichia coli. the deduced amino acid sequence of each protein is also well conserved in the two bacteria. the g + c content of the m. capricolum genes is 29%, which is much lower than that of e. coli (51%). the codon usage pattern of m. c ... | 1987 | 3481422 |
| occurrence of unmodified adenine and uracil at the first position of anticodon in threonine trnas in mycoplasma capricolum. | codon usage pattern in the threonine four-codon (acn) box in mycoplasma capricolum is strongly biased towards adenine and uracil for the third base of codons. codons ending in uracil or adenine, especially acu, predominate over acc and acg. this bacterium contains two isoacceptor threonine trnas having anticodon sequences agu and ugu, both with unmodified first nucleotides. it would thus appear that acn codons are translated in an unusual way; trna(thr)(agu) would translate the most abundantly u ... | 1987 | 3502716 |
| pathogenicity of mycoplasma capricolum in sheep after experimental infection. | mycoplasma (m.) capricolum has been frequently isolated from diseased as well as from healthy sheep in morocco. in order to determine its pathogenicity for sheep, experiments were performed in three trials with the moroccan isolate 012. the following results were obtained: ewes inoculated intramammarily developed acute mastitis; the organism was transmitted to lambs suckling these ewes. only a mild mastitis appeared after a second inoculum, performed 5 weeks after the first. young lambs, 1 month ... | 1987 | 3660581 |
| comparison of caprine mycoplasmosis caused by mycoplasma capricolum, mycoplasma mycoides subsp. mycoides, and mycoplasma putrefaciens. | we reviewed the disease process in goats caused by mycoplasma capricolum, m. mycoides subsp. mycoides, and m. putrefaciens and compared mycoplasma-infected goats (particularly young kids) for microanatomic changes. m. capricolum and m. mycoides subsp. mycoides cause a nearly identical disease: septicemia leading to pyrexia, high morbidity and mortality, interstitial pneumonia, arthritis (including polyarthritis), and mastitis. until recently, m. putrefaciens was known only as a cause of mastitis ... | 1987 | 3667229 |
| spiroplasmas: gene structure and expression. | upon sequencing of the spv4 genome, eight putative open reading frames (orfs) including that for the 65-kilodalton (kda) capsid protein were detected. they involve all three reading frames. three promoter sequences were found, as well as a transcription terminator and the initiation site for complementary strand synthesis. ribosome binding sites and regulatory sequences are closely related to those of eubacteria. codon usage analysis showed that a and t terminated codons are preferably used. uaa ... | 1987 | 3667235 |
| ultrastructural changes in mollicutes induced by the peptide antibiotic herbicolin a. | electron microscopy of negatively stained mycoplasma, ureaplasma, and acholeplasma cells showed ultrastructural changes after 10 min of treatment of the organisms with the peptide antibiotic herbicolin a in concentrations ranging from 10 micrograms/ml for mycoplasma capricolum to 600 micrograms/ml for ureaplasma urealyticum. the morphological changes were shown to be reversible at low concentrations of the antibiotic but irreversible at high concentrations. | 1986 | 3717939 |
| changes in membrane lipid composition of mycoplasma capricolum affect the cell volume. | the cellular water volume of mycoplasma capricolum was markedly increased by a decrease in the cholesterol-to-phospholipid molar ratio in the membrane. an increase in cell volume was also observed with the increase in the phospholipid cell membrane content obtained by the incorporation of exogenous phosphatidylcholine from the growth medium. | 1986 | 3745117 |
| lipid interconversions in aging mycoplasma capricolum cultures. | during the progression of mycoplasma capricolum cultures from the early exponential to the stationary phase of growth, a decrease in the phospholipid-to-protein ratio and increases in both the unsaturated-to-saturated fatty acid ratio and the diphosphatidylglycerol (dpg)-to-phosphatidylglycerol (pg) ratio were found. the freedom of motion of spin-labeled fatty acids incorporated into the membrane remained unchanged throughout the growth cycle. the increase in dpg was almost stoichiometric with t ... | 1986 | 3745126 |
| gene organization of mycoplasma capricolum. | | 1986 | 3751721 |
| isolation of mycoplasma capricolum from cows with mastitis. | | 1986 | 3765306 |
| uga is read as tryptophan in mycoplasma capricolum. | uga is a nonsense or termination (opal) codon throughout prokaryotes and eukaryotes. however, mitochondria use not only ugg but also uga as a tryptophan codon. here, we show that uga also codes for tryptophan in mycoplasma capricolum, a wall-less bacterium having a genome only 20-25% the size of the escherichia coli genome. this conclusion is based on the following evidence. first, the nucleotide sequence of the s3 and l16 ribosomal protein genes from m. capricolum includes uga codons in the rea ... | 1985 | 3887399 |
| a change in the genetic code in mycoplasma capricolum. | mycoplasma capricolum was previously found to use uga instead of ugg as its codon for tryptophan and to contain 75% a + t in its dna. the codon change could have been due to mutational pressure to replace c + g by a + t, resulting in the replacement of uga stop codons by uaa, change of the anticodon in tryptophan trna from cca to uca, and replacement of ugg tryptophan codons by uga. none of these changes should have been deleterious. | 1985 | 3936937 |
| purification and properties of an fad-containing nadh oxidase from mycoplasma capricolum. | from the prokaryotic microorganism mycoplasma capricolum an fad-containing nadh oxidase has been purified by preparative fplc to homogeneity, as judged by polyacrylamide gel electrophoresis. the apparent molecular mass of the enzyme was found to be 72.5 kda, with an isoelectric point of 5.2, and no detectable subunits. no iron, copper, manganese or molybdenium could be detected. on the basis of a minimum molecular mass of 72.5 kda a ratio of fad/protein of 1:1 could be derived. its amino-acid co ... | 1985 | 4063067 |
| the mycoplasmacidal effect of herbicolin a. | this study was to determine if the inhibitory effect of herbicolin a to sterol-requiring members of the class mollicutes is due to a mycoplasmastatic or a mycoplasmacidal effect. two strains were used as test organisms, mycoplasma capricolum (california kid) and ureaplasma urealyticum (t-960). following exposure of the mycoplasmas to various inhibitory concentrations of herbicolin a for various periods of time, elimination of the antibiotic as a preliminary to demonstration of any surviving orga ... | 1985 | 4066560 |
| characterization of some caprine mycoplasmas, with proposals for new species, mycoplasma capricolum and mycoplasma putrefaciens. | | 1974 | 4612102 |
| phosphate distribution and transport in mycoplasma. | in mycoplasma capricolum 75% of the total cell phosphate is found in the nucleic acids and polyphosphate fraction. the cold acid soluble fraction consisted primarily of orthophosphate (40 mm) in resting organisms. in metabolizing cells, phosphate esters, probably organophosphates, represented the largest part of that fraction (40 mm) whose content in orthophosphate is lowered to 10-15 mm. phosphate is accumulated against its concentration gradient after its entry as orthophosphate. it is taken u ... | 1984 | 6084546 |
| molecular cloning of ribosomal protein genes from mycoplasma capricolum. | a bg/ii-fragment from the mycoplasma capricolum dna cloned into pbr322 has been found to contain a cluster of ribosomal protein genes. the recombinant plasmid, pmcb1088, includes a 9 kilobase-pair insert that codes for at least eight ribosomal proteins of m. capricolum. the protein genes are expressed in escherichia coli cells. | 1984 | 6094981 |
| preferential use of a- and u-rich codons for mycoplasma capricolum ribosomal proteins s8 and l6. | the nucleotide sequence of the 1.3 kilobase-pair dna segment, which contains the genes for ribosomal proteins s8 and l6, and a part of l18 of mycoplasma capricolum, has been determined and compared with the corresponding sequence in escherichia coli (cerretti et al., nucl. acids res. 11, 2599, 1983). identities of the predicted amino acid sequences of s8 and l6 between the two organisms are 54% and 42%, respectively. the a + t content of the m. capricolum genes is 71%, which is much higher than ... | 1984 | 6095194 |
| methylation patterns of mycoplasma transfer and ribosomal ribonucleic acid. | the methylation patterns of transfer and ribosomal ribonucleic acid (rna) from two mycoplasmas, mycoplasma capricolum and acholeplasma laidlawii, have been examined. the transfer rna from the two mycoplasmas resembled that of other procaryotes in degree of methylation and general diversity of methylated nucleotides, and bore particular resemblance to bacillus subtilis transfer rna. the only unusual feature was the absence of m5u from m. capricolum transfer rna. the methylation patterns of the my ... | 1980 | 6160144 |
| stable rna synthesis and its control in mycoplasma capricolum. | the synthesis of stable rna in mycoplasma capricolum was studied by [32p] labeling of cellular rna of cells grown in a partially-defined medium in the presence or absence of an amino acid mixture supplement. the results indicate that m. capricolum employs the same stringent control mechanism used by e. coli cells, as judged by a decreased synthesis of stable rna and accumulation of 5'-triphosphoguanosine-3'-diphosphate (pppgpp) and 5'-diphosphoguanosine-3'-diphosphate (ppgpp) in response to amin ... | 1981 | 6169010 |
| coordinate regulation of unsaturated phospholipid, rna, and protein synthesis in mycoplasma capricolum by cholesterol. | the effect of cholesterol, epicoprostanol, and phosphatidylcholine on phospholipid, rna, and protein synthesis was investigated in the sterol auxotroph mycoplasma capricolum. cells growing poorly on lanosterol were stimulated to grow more rapidly by supplementing the medium with either 2 micrograms of cholesterol or 2.2 micrograms of egg phosphatidylcholine per ml. in such cells cholesterol caused a sequential stimulation of phospholipid, rna, and protein synthesis. enhanced oleate incorporation ... | 1983 | 6187002 |
| mycoplasmas (mollicutes) have a low number of rrna genes. | dna from mycoplasma, ureaplasma, acholeplasma, and spiroplasma species digested by restriction endonucleases was hybridized with probes consisting of portions of the rrnb rrna operon of escherichia coli and the rrna operon of mycoplasma capricolum. the results indicate the presence of only one or two sets of rrna genes in the genome of mollicutes linked in the procaryotic fashion, 16s-23s-5s. | 1984 | 6201476 |
| the ribosomal genes of mycoplasma capricolum. | the nucleotide sequence of 5s rrna from mycoplasma capricolum is more similar to that of the gram-positive bacteria than that of the gram-negative bacteria. the presence of two copies of rrna genes in m. capricolum genome has been demonstrated. the two different rrna gene clusters have been cloned in e. coli plasmid vectors and analyzed for the rrna gene organizations, demonstrating that the gene arrangement is in the order of 16s, 23s, and 5s rdna. the ribosomes of m. capricolum contain about 3 ... | 1983 | 6206654 |
| organization of ribosomal rna genes in mycoplasma capricolum. | dna segments carrying rrna genes of mycoplasma capricolum have been cloned and characterized by restriction endonuclease mapping, dna-rna hybridization and nucleotide sequencing. the m. capricolum genome has two sets of rrna gene clusters, where the arrangement is in the order of (5')16s-23s-5s(3'). the spacer region between 16s and 23s rdna is extremely rich in at and does not carry any trna genes. | 1984 | 6208457 |
| nucleotide sequence of the rrnb 16s ribosomal rna gene from mycoplasma capricolum. | the nucleotide sequences of the rrnb 16s ribosomal rna gene and its 5'-and 3'-flanking regions from mycoplasma capricolum have been determined. the coding sequence is 1521 base pairs long, being 21 base pairs shorter than that of the escherichia coli 16s rrna gene. the 16s rrna sequence of m. capricolum reveals 74% and 76% identify with that of e. coli and anacystis nidulans, respectively. the secondary structure model constructed from the m. capricolum 16s rrna gene sequence resembles that prop ... | 1984 | 6208458 |
| effect of cholesterol on phospholipid, rna, and protein synthesis in mycoplasma capricolum. | the regulatory role of cholesterol on phospholipid, rna and protein synthesis was investigated in mycoplasma capricolum. the addition of 2 micrograms/ml of cholesterol to cells growing slowly on lanosterol (10 micrograms/ml) caused an early stimulation of unsaturated phospholipid synthesis followed by a later stimulation of rna and protein synthesis. epicoprostanol, a cholesterol antagonist, sequentially inhibited these three processes. phospholipid served as the acyl donor for the acylation of ... | 1984 | 6210267 |
| the number of ribosomal rna genes in mycoplasma capricolum. | we have examined the number of rrna genes in mycoplasma capricolum (kid) by hybridization of bg/ii-, ecori- and xbal-digests of dna to [3'-32p] 16s, 23s and 5s rrnas according to the southern procedure (1975). all the restriction gels gave two radioactive bands with three kinds of rrna. furthermore, band positions were indistinguishable from one another when 16s, 23s and 5s rrnas were used as probes, indicating that each band contains sequences corresponding to the 3'-termini of 16s, 23s and 5s ... | 1981 | 6272066 |
| phospholipid interconversions in mycoplasma capricolum. | mycoplasma capricolum cells increase their phospholipid content by incorporating exogenous phospholipids from the growth medium. growing the cells in media with increasing serum concentrations resulted in a massive incorporation of phosphatidylcholine and sphingomyelin (up to about 50% of total phospholipids) into the cell membrane. the incorporation of the exogenous phospholipids had essentially no effect on the rate of cell growth and did not decrease the overall phospholipid biosynthesis of t ... | 1982 | 6277632 |
| ribosomal rna genes in mycoplasma. | using southern blotting analysis with labelled mycoplasmal ribosomal rna as probe, two fragments (1 kb and 5 kb) were detected in an ecor i digest of mycoplasma capricolum dna. this analysis revealed that the 5 kb fragment carries both 16s rrna sequences and the entire 23s rrna gene of this mycoplasma. the 1 kb fragment contains 16s rrna sequences only. the 5 kb ecor i fragment has been cloned and used to characterize the structure of rrna cistrons in various mycoplasma strains. these experiment ... | 1982 | 6289267 |
| cholesterol and phospholipid uptake by mycoplasmas. | mycoplasma offer several unique advantages for investigating the mechanism controlling transfer and uptake of exogenous cholesterol and phospholipids by biomembranes, as their plasma membrane interacts directly with exogenous lipid donors and their endogenous lipid synthesis is restricted. growing cells of five species of mycoplasma were found to take up significant quantities of phosphatidylcholine and sphingomyelin as well as free and esterified cholesterol. in contrast, growing cells of three ... | 1982 | 6289408 |
| physical mapping of the ribosomal rna genes of mycoplasma capricolum. | physical mapping of the rrna genes of mycoplasma capricolum was done by digestion of the mycoplasmal dna with ecori, psti and bglii and hybridization with nick-translated probes consisting of defined portions of the rrnb ribosomal rna operon of escherichia coli. the results indicate that the rrna genes in the chromosome of m. capricolum are arranged in two clusters, each organized in the order 5'-16s-23s-5s-3', resembling the order of the genes in the rrnb operon, with no large spacer regions se ... | 1984 | 6324119 |
| sterol synergism in yeast. | sterol synergism as previously observed [dahl, c.e., dahl, j.s. & bloch, k. (1980) biochemistry 19, 1462-1467] and defined as a greater-than-additive growth response to pairs of sterols by mycoplasma capricolum [dahl, j.s., dahl, c.e. & bloch, k. (1981) j. biol. chem. 256, 87-91] is now demonstrated in the yeast mutant gl7, which is auxotrophic for sterol and unsaturated fatty acid. mutant cells growing poorly when provided with cholesterol and oleic acid respond to ergosterol supplements (ergos ... | 1983 | 6338497 |
| electrophoretic analysis of proteins from mycoplasma capricolum and related serotypes using extracts from intact cells and from minicells containing cloned mycoplasma dna. | the acidic proteins of six different mycoplasma serotypes causing bovine or caprine pleuropneumonia were compared by two-dimensional gel electrophoresis of extracts of 35s-labelled cells. the organisms investigated were mycoplasma mycoides subsp. mycoides (pg1), m. mycoides subsp. mycoides (y-goat), m. mycoides subsp. capri (pg3), m. capricolum (california kid), the unclassified bovine serogroup 7 of leach (pg50) and the f38-like group (f38). the results suggested a close relationship between m. ... | 1984 | 6384426 |
| a study of f38-type and related mycoplasmas by mycoplasmaemia and cross-immunization tests in mice. | in vivo methods were used to study the f38-type mycoplasma in parallel with related mycoplasmas. three of five strains of 'bovine serogroup 7' with an unknown history of subculture produced mycoplasmaemia in mice inoculated intraperitoneally. a strain of 'bovine serogroup l' also produced mycoplasmaemia, but no evidence of similar ability could be found for single strains of mycoplasma capricolum, m. equigenitalium and m. primatum, or for two strains of the f38-type mycoplasma. in cross-immuniza ... | 1984 | 6392418 |
| septicaemia and pneumonia in mycoplasma capricolum infections of young goats. | | 1984 | 6497801 |
| pathogenicity of mycoplasma capricolum and mycoplasma putrefaciens. | mycoplasma capricolum causes high morbidity and mortality in goats. young kids fed a one-time oral dose of greater than or equal to 1 x 10(5) colony-forming units of the gm13 isolate usually died during the septicemic phase. the cardinal lesions were a fibrinopurulent polyarthritis and an acute, diffuse interstitial pneumonia. lactating goats exposed to low numbers of the organism via the teat canal experienced similar lesions and acute mastitis, agalactia, and hardened udders. the intramammary ... | 1984 | 6511323 |
| phospholipids as acyl donors to membrane proteins of mycoplasma capricolum. | mycoplasma capricolum, a procaryotic sterol and fatty acid auxotroph, contains a large number of membrane proteins covalently modified by both saturated and unsaturated fatty acids (dahl, c.e., dahl, j.s., and bloch, k. (1983) j. biol. chem. 258, 11814-11818). pulse-chase experiments show that the radioactivity in the fatty acid moieties of the acyl proteins increases rather than decreases during a 4.5-h incubation period suggesting that a large intracellular pool of metabolites such as phosphol ... | 1984 | 6547954 |
| proteolipid formation in mycoplasma capricolum. influence of cholesterol on unsaturated fatty acid acylation of membrane proteins. | mycoplasma capricolum, a procaryotic sterol and fatty acid auxotroph was grown on media supplemented with [3h]palmitate or [3h]oleate. the isolated bacterial membranes were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis. of the more than 50 membrane polypeptides revealed by coomassie blue staining, approximately 25 were labeled with [3h]palmitate and only about 6 were labeled with [3h]oleate. exhaustive delipidation of the membranes with chloroform:methanol did not alter ... | 1983 | 6619146 |