| characterization of membrane and cytoplasmic antigens of mycoplasma arginini by two-dimensional (crossed) immunoelectrophoresis. | two-dimensional immunoelectrophoresis was employed to electrophoretically identify membrane and cytoplasmic antigens of mycoplasma arginini g-230. five distinct cytoplasmic antigens were observed in soluble fractions prepared by digitonin lysis with electrophoretic mobilities (relative to bovine albumin) ranging from 0.36 to 0.86; four of these were common to other m. arginini strains: leonis and 23243. five membrane antigens were identified, two of which (0.4 and 0.2) were common to the other m ... | 1977 | 64457 |
| detection of mycoplasma contamination in tissue cultures by fluorescence microscopy. | the in situ staining method of chen (1977) for the detection of mycoplasma contaminants in tissue cultures was tested in cultures of human skin fibroblasts after controlled contamination with mycoplasma arginini. it is concluded that this method is reliable only at infection rates of 100% or higher, i.e., at one mycoplasma or more per tissue-culture cell. | 1979 | 86495 |
| suppression of lymphocyte activation by a factor produced by mycoplasma arginini. | mycoplasma arginini, when grown in broth or in cultures of human lymphoblast cell lines, inhibits activation of lymphocytes by allogeneic cells of mitogens. this inhibition can also be produced by cell free media obtained after the growth of this organism. inhibition is not species specific and inhibits both b and t cell activation in the mouse. the inhibitory factor is required during the first 3 days of the mixed leukocyte culture, but has no effect on the latter phase of this reaction when t ... | 1975 | 127011 |
| the incidence of mycoplasma dispar, ureaplasma and conventional mycoplasma in the pneumonic calf lung. | this report describes the incidence of mycoplasma dispar, ureaplasma and conventional (large colony) mycoplasma isolated from the pneumonic lungs of groups of young calves and the identification to species level of mycoplasmas in mixed populations with the aid of the indirect fluorescent antibody test. pneumonic lung tissue yielded one or more mycoplasma species from 88% of the 153 calves cultured. the mycoplasmas identified and percent of the calves with lungs positive for each species were: m. ... | 1979 | 398234 |
| application of charge shift electrophoresis to antigenic analysis of mycoplasmic membranes by two-dimensional (crossed) immunoelectrophoresis. | both the anodic migration and resolution of membrane antigens of mycoplasma arginini were greatly improved in two-dimensional immunoelectrophoresis by incorporating both a neutral detergent, triton x-100, and an anionic detergent, sodium deoxycholate, in the agarose support for first phase electrophoresis. the mobility of cytoplasmic antigens was unaffected at concentrations of 0.1% or less of sodium deoxycholate. | 1978 | 669825 |
| isolation of mycoplasma arginini from swine and from a swine waste disposal system. | mycoplasma arginini was isolated from the pharynges and tonsils of 4 of 6 swine necropsied during an epizootic of respiratory tract diseases and lameness. the organism was also isolated from 2 of 16 asymptomatic swine examined in the same herd approximately 10 months after the epizootic. in addition, m arginini, acholeplasma laidlawii, and other acholeplasmas were isolated from recycled effluent of an anaerobic lagoon used for disposal of waste from the swine herd. this observation represents th ... | 1978 | 677536 |
| differences in incorporation of nucleic acid bases and nucleosides by various mycoplasma and acholeplasma species. | eight species representative of the serological diversity of the mycoplasmatales were tested for their ability to incorporate radiolabeled nucleic acid precursors into acid-insoluble material. cultures in complex growth medium were centrifuged and resuspended in minimal essential medium (eagle). for acholeplasma laidlawii, labeling occurred mainly during the first 4 h of incubation, with substrate saturation at 20 micron. all organisms tested incorporated uracil, adenine, and guanine; none incor ... | 1978 | 681280 |
| significance and pathogenic role of mycoplasma arginini in cat diseases. | eighty-one strans of mycoplasma arginini were isolated from swabs and tissues cultured from a series of 555 cats. the majority of these strains (84%) were recovered from the oropharyngeal regions. m. arginini was experimentally inoculated into young kittens. no clinical disease was produced. however, there was rapid colonization in the inoculated sites. | 1977 | 907911 |
| bronchiectasis in yearling feedlot cattle. | thoughout all of 1974, we surveyed, for illnesses and deaths, about 407,000 yearling feedlot cattle. from the 3,943 dead cattle, 1,988 necropsies were made; of the cattle necropsied, 32 (1.6%) had bronchiectasis. in this disease, the permanently dilated small bronchi and bronchioles, located in ventral parts of the lungs, were filled with accumulations of exudate and microorganisms, including pasteurella hemolytica, pasteurella multocida, corynebacterium pyogenes, escherichia coli, salmonella an ... | 1976 | 956033 |
| the prevalence of mycoplasma ovipneumoniae and mycoplasma arginini in the respiratory tract of sheep. | | 1975 | 1058377 |
| mycoplasma species recovered from the reproductive tracts of western canadian cows. | samples of cervico-vaginal mucus from 633 animals from 110 herds were cultured and yielded the following mycoplasmas: t-strain--88: mycoplasma bovigenitalium--79, mycoplasma spp. (leach group 7)--7, acholeplasma laidlawii--4, mycoplasma bovirhinis--2 and one not typable. uterine exudates and endometrial scrapings from 80 infertile cows in two herds were examined. four animals were positive, m. bovigenitalium was isolated three times, a. laidlawii and mycoplasma arginini once each. sixty-five nor ... | 1975 | 1125831 |
| direct isolation of mycoplasmas and acholeplasmas from sera and kidneys of calves. | kidneys and sera of calves from various farms and primary kidney tissue cultures were tested for mycoplasma and acholeplasma contamination. altogether 24 strains belonging to mycoplasma arginini and acholeplasma axanthum were isolated from tissue cultures, kidneys and sera. the same species were detected from lungs and peribronchial lymph nodes of calves, together with a. laidlawii, a. modicum and m. bovirhinis species. there was a close correlation between the mycoplasma content of tissue cultu ... | 1975 | 1167436 |
| antigenic analysis of three strains of mycoplasma arginini by two-dimensional immunoelectrophoresis. | the occurrence of species-specific and strain-specific antigens in three strains of mycoplasma arginini (g-230, leonis and 23243) was studied by two-dimensional immunoelectrophoresis. approximately 20 antigenic components could be detected in each strain. it was possible to analyze 6 to 7 major and distinct components from each strain by two techniques: "enhancement" where antigen to an additional strain is added to the first phase of the electrophoresis which increases the size of common peaks ... | 1975 | 1167566 |
| drying and irradiation of calf and horse serum. ii. influence on mycoplasma content of the calf sera. | drying of calf sera produced only a negligible change in their content of acholeplasma laidlawii organisms and reduced the content of mycoplasma arginini organisms by 2 to 4 logs. gamma-irradiation of liquid and dried calf sera killed m. arginini and a. laidlawii organisms at irradiation levels of 0.2-0.3 and 0.4-0.6 mrads, respectively. | 1975 | 1179873 |
| mycoplasmas and cuffing pneumonia in a group of calves. | the mycoplasmas found in the lungs of 20 calves, housed together for six months, and the related pulmonary pathology are reported. twelve calves had cuffing pneumonia and in this group there was a significantly higher isolation frequency of mycoplasma dispar and ureaplasma spp compared with the non-pneumonic group. mycoplasma bovirhinis and acholeplasma laidlawii were isolated from the lungs of calves in both groups. mycoplasma arginini was not recovered from the lungs of any calf. the significa ... | 1975 | 1198879 |
| role of arginine deiminase in growth of mycoplasma hominis. | arginine has been considered as the major energy source of nonglycolytic arginine-utilizing mycoplasmata. when three strains of mycoplasma arginini, and one strain each of mycoplasma arthritidis, mycoplasma fermentans, mycoplasma gallinarum, mycoplasma gallisepticum and mycoplasma hominis were grown in the medium with high arginine concentration (34 mm) compared with low arginine (4 mm), both the protein content of the organisms and the specific activity of arginine deiminase increased. m. ferme ... | 1976 | 1262306 |
| [identification of mycoplasma by the growth inhibition of samples isolated from cell cultures]. | cell cultures must be continuously screened for the presence of mycoplasma because, although these microorganisms sometimes pass unnoticed, they may cause chromosomic alterations and interfere with viral replication, antibody and interferon production etc. the international organization for mycoplasmology (iom) recommends the isolation and identification of mycoplasma with a view to the detection of the origin of the infection and the improvement of the quality of the cultures. in this paper, 37 ... | 1992 | 1307416 |
| [mycoplasma restriction-modification system muni and its possible role in pathogenesis processes]. | the restriction-modification system, named rmmuni, has been purified and characterised from friend murine erythroleukemia cells. the site-specific endonuclease recognizes and cleaves the 5'c1aattg nucleotide sequence. rmuni is an isoschizomer of rmfei from mycoplasma fermentans. site-specific methylase modifies the second adenine residue in the same sequence (5'cam6attg). it was established that the discovered enzymatic system is from mycoplasma which contaminates cell lines. mycoplasma's dna hy ... | 1992 | 1406610 |
| [the effect of mycoplasma contamination on the karyotypic structure of a skin fibroblast cell line from the indian muntjac]. | the karyotypic variability has been studied in a low chromosomal cell line of the indian muntjak skin fibroblasts contaminated with mycoplasma arginini r-16 and acholeplasma laidlawii a. the mycoplasmal contamination exerted influence on the cell distribution for the chromosome number. the frequency of the modal class cells with 7 chromosomes, having the main structure variant of the karyotype (msvk) 2 + 2 + 1 + 1 + 1, was seen to decrease, while the frequency of the submodal class cells with 6 ... | 1992 | 1440934 |
| [rotrac capillary pore membranes for laboratory filtration. ii. bacteria-free filtration]. | because of their special characteristics capillary pore membranes (cpm) are now applied in several branches of separation techniques and analytics. besides applications in particle analytics and microfiltration of different media capillary pore membranes can be used in microorganism separation. it was shown that rotrac cpm can be used for bacteria free (or so called sterile) filtration. acceptable fluxes were reached in separation of pseudomonas diminuta (test species atcc 19146). membranes with ... | 1992 | 1457036 |
| fatal septicemia due to mycoplasma arginini: a new human zoonosis. | a 64-year-old slaughterhouse worker with advanced non-hodgkin's lymphoma developed septicemia and pneumonia. mycoplasma arginini, a wall-free prokaryote found in a variety of domestic animal hosts, was repeatedly isolated from blood and bronchial washings from the patient. immunosuppression, in part caused by hypogammaglobulinemia, probably played a key role in predisposing the patient to a fatal infection. this case suggests that animal mycoplasmas should be considered in the list of infectious ... | 1992 | 1520790 |
| arginine deiminase gene of an aids-associated mycoplasma, mycoplasma incognitus. | recently, we have demonstrated by molecular cloning that a strong immunosuppressive factor derived from mycoplasma arginini is arginine deiminase. we show here that mycoplasma species identified in many of aids patients also bear the arginine deiminase gene. the implication of the arginine deiminase gene detected in aids-associated mycoplasma species is discussed. | 1992 | 1522818 |
| in vivo anti-tumor activity of arginine deiminase purified from mycoplasma arginini. | arginine deiminase (ec 3.5.3.6) was purified to homogeneity from the cell extract of mycoplasma arginini by molecular-sieve, anion-exchange and arginine-affinity chromatographies. the purified enzyme was composed of 2 identical sub-units with a molecular weight of 45.000 and had a pi of 4.7. its vmax value and km value for l-arginine were estimated to be 50 units/mg protein and 0.2 mm, respectively. it exerted maximal enzyme activity at ph 6.0-7.5 and at 50 degrees c. the arginine deiminase was ... | 1992 | 1568792 |
| no direct neuronotoxicity by hiv-1 virions or culture fluids from hiv-1-infected t cells or monocytes. | macrophages and microglia are the principal target cells for human immunodeficiency virus (hiv) in brain, and as such, are likely participants in the neuropathology of hiv infection. in a model system for this process, we found that fluids from human monocyte cultures enhanced survival and differentiation of the neurons in fetal rat brain explants. in contrast, fluids from hiv-infected monocyte cultures were strongly toxic to neurons and paradoxically enhanced the proliferation of glial cells. f ... | 1992 | 1599756 |
| detection of mycoplasma contamination through modulation (stimulation or inhibition) of thymidine incorporation by unstimulated mouse spleen cells. | in the present report we describe a rapid and sensitive assay for mycoplasma detection in cell cultures. the assay is based on the ability of contaminated culture supernatants to modulate [3h]tdr incorporation by unstimulated mouse splenocytes. several mycoplasma species (mycoplasma orale, culturable and non-culturable strains of mycoplasma hyorhinis) inhibited [3h]tdr incorporation and permitted the detection of some contaminated cell cultures that would otherwise have escaped detection in assa ... | 1992 | 1640109 |
| fast and simple procedure for the detection of cell culture mycoplasmas using a single monoclonal antibody. | the detection of mycoplasmas in cell cultures is still a problem, especially in those laboratories in which the detection and identification of microorganisms is not established as a routine procedure. in our laboratory, monoclonal antibodies (mabs) have been prepared to acholeplasma laidlawii, mycoplasma hyorhinis, mycoplasma orale, mycoplasma arginini and mycoplasma salivarium. 30 mabs were obtained and one of these, designated ccm-2, was shown to bind to all five mycoplasma species. it also b ... | 1990 | 1697315 |
| identification of a mycoplasmal protein which binds immunoglobulins nonimmunologically. | immunoblotted protein samples from several strains of mycoplasma hominis and from one strain of mycoplasma arginini each contain a polypeptide of a molecular mass of 95,000 to 105,000 da which binds immunoglobulin nonimmunologically. immunoblots from these organisms were probed with alkaline phosphatase-conjugated goat anti-rabbit immunoglobulin, conjugated goat immunoglobulin g (igg) fab fragments, and conjugated goat igg fc fragments. the polypeptide bound the goat anti-rabbit molecules and th ... | 1991 | 2037376 |
| differential induction of bone marrow macrophage proliferation by mycoplasmas involves granulocyte-macrophage colony-stimulating factor. | we have studied the ability of three different mycoplasma species to induce proliferation of bone marrow-derived macrophages (bmm). we observed a significant mitogenic effect when bmm cells from balb/c, dba/2j, sjl, and c57bl/6 mice were incubated with membranes derived from mycoplasma arginini or m. arthritidis but not when they were incubated with an equivalent amount of m. pulmonis membrane. we also determined that pretreatment of mycoplasma membrane preparations with papain eliminated the ab ... | 1990 | 2228227 |
| cloning and nucleotide sequence of the gene encoding arginine deiminase of mycoplasma arginini. | the existence of a mycoplasmal arginine deiminase which catalyzes the conversion of l-arginine to l-citrulline has been postulated. here we show the partial amino acid sequence of arginine deiminase of mycoplasma arginini and the complete nucleotide sequence of the arginine deiminase gene of m. arginini. the open reading frame deduced from this sequence consists of 1,230 bp encoding 410 amino acids. the mature form of this enzyme contains 409 amino acids after the deletion of the first methionin ... | 1990 | 2228248 |
| differences in accumulation of radiolabeled amino acids and polyamines by mycoplasma and acholeplasma species. | four species in the order mycoplasmatales, mycoplasma capricolum, mycoplasma hominis, mycoplasma arginini, and acholeplasma laidlawii, were compared for their ability to accumulate radiolabeled amino acids and polyamines. the use of a novel high-molecular-weight (hmw) medium, from which molecules of less than 12,000 molecular weight had been removed by extensive dialysis, allowed us to discern significant differences among the species in their relative accumulations of [3h]methionine and [3h]leu ... | 1990 | 2275861 |
| cloning and sequence analysis of the arginine deiminase gene from mycoplasma arginini. | arginine deiminase from mycoplasma arginini was purified. the purified enzyme has a molecular weight of 46,000 daltons as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. its specific activity (20 units/mg protein) and amino acid composition showed a strong similarity to that of the mycoplasma arthritidis arginine deiminase. the amino acid sequences of the n-terminal region and three internal peptides generated by enzymatic cleavage of the purified protein were determined ... | 1990 | 2325633 |
| identification and purification of arginine deiminase that originated from mycoplasma arginini. | a lymphocyte blastogenesis inhibitory factor, (lbif), was purified from the culture supernatant of human histiocytic lymphoma u937 by fast protein liquid chromatography. in this study, we demonstrated, first, that lbif originated from a mycoplasma, mycoplasma arginini, infecting u937 cells, and second, that lbif bore the arginine deiminase activity. the implication of in vivo immunosuppression induced by arginine-utilizing mycoplasma species is discussed. | 1990 | 2370103 |
| evaluation of three methods for curing hybridomas from mycoplasma contamination. | hybridomas are as susceptible to mycoplasma contamination as animal cell cultures. two in vitro methods and one in vivo passage into mice were compared for efficiency of curing hybridomas from mycoplasma contaminations. four contaminated clones were treated with the combined action of 5-bromouracil (5-brura) and the hoechst 33258 followed by photosensitization. the other in vitro method involved the use of bm-cycline. the success of overcoming the mycoplasma contamination was dependent on the le ... | 1988 | 2453449 |
| [the reasons for the occurrence of non-specific reactions in the immunodiffusion test for enzootic bovine leukosis]. | a batch of enzootic bovine leucosis antigen earmarked for immunodiffusion testing exhibited unspecific responses to cattle sera, which prompted an investigation of the underlying causes. evidence was produced, for the first time ever, that such reactions were attributable to an antigen-antibody reaction caused by mycoplasma arginini. | 1989 | 2549905 |
| interactions between mycoplasmas and the immune system. | mycoplasmas are a heterogenous group of prokaryotic organisms causing a wide variety of diseases, including autoimmune disorders. thus, it is not surprising that various mycoplasmas strains, including mycoplasma arginini, m. arthritidis, m. neurolyticum and m. pulmonis, are able to regulate the immune response. though some of the studies of the immunomodulatory action of mycoplasmas have been done in vivo, the majority of the investigations have been conducted in vitro. this has led to the recog ... | 1989 | 2691389 |
| elimination of mycoplasmas from mouse myeloma cells by intraperitoneal passage in mice and by antibiotic treatment. | intraperitoneal passage in mice and antibiotic treatment were evaluated alone and in combination for elimination of mycoplasma contamination of mouse myeloma cell cultures. intentional infections were established by inoculating mycoplasma arginini, m. fermentans, m. hyorhinis and m. orale into cell cultures. successful elimination of mycoplasmas was achieved with all strains tested by intraperitoneal passage in mice, however, cells infected with m. hyorhinis did not survive the infection long en ... | 1989 | 2714817 |
| the regulation of murine b cell differentiation. i. nonspecific suppression caused by mycoplasma arginini. | during the screening of suppressor t cell (ts) hybridomas for antigen-nonspecific suppressive activity, we isolated a strain of mycoplasma arginini which inhibits b cell antibody production in vitro. the addition of mycoplasma-containing ts hybridoma culture supernatant to splenic b cells responding to sheep red blood cells (srbc) and t cell-replacing factor or to trinitrophenyl-lipopolysaccharide (tnp-lps) suppressed the production of anti-srbc and anti-tnp plaque-forming cells in a dose-depend ... | 1989 | 2790965 |
| [decontamination of continual cell lines spontaneously infected with mycoplasmas]. | the continual cell lines of bovine kidneys mdbk and aubek, and porcine kidneys rpd and ibrs, spontaneously infected with mycoplasma arginini and acholeplasma laidlawii, were decontaminated by the method of selective elimination. two elimination procedures were modified to be used for the decontamination: one based on the reduction of infection by the light treatment of the cultures, the other based on the selection of mycoplasma-free cell population through cell clonation. on the basis of a long ... | 1986 | 3090766 |
| host immunity to mycoplasma antigens introduced into b16 melanoma cells: effect on tumor growth rate and metastasis. | immunization of syngeneic c57bl/6 mice with x-irradiated b16 melanoma cells was previously shown to elicit antibodies specific to viral antigens on the melanoma cells. when immunized mice were challenged with viable subcutaneous transplants of b16 melanoma cells that formed non-metastatic tumors in normal mice, tumors failed to develop in some mice, but there was a high incidence of lung metastasis in mice with progressively growing tumors. to determine whether protective immunity and/or enhance ... | 1988 | 3129225 |
| [detection and identification of mycoplasma infections by dna hybridization]. | infection of cell cultures by mycoplasmas can be detected by hybridization of the dna of suspected cell cultures with recombinant plasmids containing fragments of the mycoplasma dna. the test is very sensitive and allows detection of as little as 1 ng of mycoplasmal dna, roughly equivalent to the dna amount of 10(6) mycoplasmas. this approach turns out to be effective for detection and identification of mycoplasmas in clinical material, plant and insect tissues. a set of dna probes for detection ... | 1987 | 3318046 |
| infectious agents in respiratory disease of housed, fattening lambs in northern ireland. | a two-year longitudinal, microbiological and pathological survey of respiratory disease in lambs housed for fattening at three-and-a-half to four months of age was undertaken. in the first year samples of nasal mucus and blood were taken from lambs each week for the first nine weeks after entry to a fattening unit and each week one lamb was examined post mortem. in the second year two additional fattening units were included in the survey, when samples of blood and nasal mucus were taken from la ... | 1988 | 3381463 |
| the occurrence of mycoplasma conjunctivae in the netherlands and its association with infectious keratoconjunctivitis in sheep and goats. | the occurrence of mycoplasma conjunctivae in the netherlands is reported for the first time. m. conjunctivae was detected in all five sheep flocks examined with clinical signs of infectious keratoconjunctivitis (ikc) and in none of five sheep flocks without these clinical signs. these observations suggest a primary aetiological role of this organism in ikc in the netherlands. m. conjunctivae was also detected on a goat farm with clinical signs of ikc. experiences in culturing this organism are r ... | 1988 | 3413973 |
| enhancement of immunoglobulin secretion by the lymphokine-like activity of a mycoplasma arginini strain. | b lymphocytes, preactivated by lipopolysaccharide (lps), could be triggered to growth by a strain of mycoplasma arginini, while the level of immunoglobulin (ig) secretion, quantitated as the number of plaque-forming cells (pfc), was low. the pfc response could be increased by the addition of conditioned media (cm) from lectin-activated spleen cells or t cell tumour el-4 to the culture of preactivated b cells. cm did not by itself induce a significant amount of pfc in the cultures of lps-preactiv ... | 1986 | 3486465 |
| [demonstration of mycoplasma in sheep pneumonia and experimental pneumonia produced by mycoplasma arginini]. | | 1987 | 3606320 |
| elimination of mycoplasma from cell cultures by means of specific bovine antiserum. | heifers were immunized against mycoplasma arginini, m. fermentans, m. hyorhinis and m. orale and the antisera were applied for elimination of these species from cell cultures. from fifteen out of nineteen contaminated human and animal cell cultures the mycoplasmas could be eliminated by treating the cells with medium with 10% or 20% antiserum (eight cases) or antiserum combined with one or two antibiotics (six cases). in ten cases two treatments were sufficient, in four cases respectively four, ... | 1987 | 3630479 |
| experimental studies on the pathogenicity of mycoplasma ovipneumoniae and mycoplasma arginini for the respiratory tract of goats. | mycoplasma ovipneumoniae and mycoplasma arginini were the species of mollicutes most commonly isolated from 175 goats with respiratory disease in ontario. the pathogenicity of m. ovipneumoniae, strain b321b and m. arginini, strain d53e, was assessed in goats following endobronchial inoculation. one out of three two year old goats developed fever after inoculation with a pure culture of strain b321b, and it had extensive subacute fibrinous pleuritis when necropsied three weeks later. neither of t ... | 1986 | 3742358 |
| the effect of mycoplasmas and acholeplasmas of equine origin on organ cultures of chicken-embryo trachea. | chicken-embryo tracheal organ cultures were inoculated with equine strains of mycoplasma arginini, m. equigenitalium, 2 strains of m. subdolum, acholeplasma laidlawii and 3 strains of a. oculi. all strains established and multiplied in the explant cultures, but only m. subdolum and a. oculi produced a cytopathic effect on ciliated epithelial cells, causing sloughing of cells and cilia after 6 days. there was a correlation between ciliostasis and increase in titre of both m. subdolum and a. oculi ... | 1985 | 3837791 |
| inhibition of [3h]thymidine incorporation by mycoplasma arginini-infected cells due to enzymatic cleavage of the nucleoside. | culture supernatants contaminated by mycoplasma arginini inhibit the incorporation of [3h]thymidine ([3h]dthd) by cytotoxic t lymphocyte cell lines. this study presents evidence that the inhibition of uptake of the nucleoside is due to the rapid cleavage of the exogenous [3h]dthd into thymine. uridine and cytidine as well as dthd are degraded by the mycoplasma-contaminated supernatants, while no cleavage was observed with uninfected supernatants. cells contaminated by mycoplasma apparently relea ... | 1985 | 3874081 |
| mycoplasma mimicry of lymphokine activity in t-cell lines. | during screening of t-t hybridomas for b-cell-stimulating factors (bsf) we found in the conditioned medium of such a line (tuh-14) an activity that turned out to cosediment with a contaminating strain of mycoplasma arginini on centrifugation. since it only affected b blasts and only to a minor extent small cells and since it did not induce immunoglobulin secretion, the mycoplasma strain seemed to mimic a bsf. m. arginini was also found in two other t-t hybridoma producer lines but not in a clone ... | 1985 | 3875144 |
| isolation of mycoplasma arginini from a goat in malaysia. | | 1985 | 3992849 |
| mycoplasma infection of cell lines can simulate the expression of fc receptors by binding of the carbohydrate moiety of antibodies. | during the production of fc receptor (fcr)-bearing hybridomas it was observed with a particular monoclonal anti-sheep red blood cell antibody (anti-srbc 1/5, igg1) that the contamination with mycoplasma arginini of in vitro cultured cell lines leads to an apparent fcr activity. this property did not correspond with the serological typing since other antibodies of the same isotype could not support fcr rosette formation. another mycoplasma strain m. orale lacked this property. analysis of the bin ... | 1985 | 3996463 |
| investigations into the possible role of mycoplasma arginini in ovine respiratory disease. | the inoculation of eight five- to seven-month-old sheep by the respiratory route with a culture of mycoplasma arginini, administered simultaneously with or two days before a culture of pasteurella haemolytica a2, did not lead to the pulmonary establishment of either organism. minor lung changes found at slaughter seven days later were therefore considered not to have been induced by the inocula. two other groups of seven sheep each were initially inoculated intratracheally with an ampicillin-tre ... | 1985 | 4012040 |
| the pathogenicity of mycoplasma ovipneumoniae and mycoplasma arginini in ovine and caprine tracheal organ cultures. | the effects of m. ovipneumoniae and m. arginini on tracheal organ cultures prepared from a neonatal kid and a foetal lamb were studied. both organisms were isolated from the cultures throughout the 14 days of observation. m. ovipneumoniae produced ciliostasis and loss of cilia, confirmed by scanning electron microscope (sem), after 4 days. these effects were sudden and profound in lamb explants, and gradual and less pronounced in kid explants. clusters of organisms attached to epithelial surface ... | 1985 | 4067018 |
| experimental mycoplasma arginini mastitis in goats. | | 1985 | 4084149 |
| isolation of mycoplasma arginini from tissue-cultures. | | 1971 | 4100177 |
| control of interferon, migration inhibition factor, and expression of virus capsid antigens in burkitt's lymphoma-derived cell lines: role of l-arginine. | the effects of an arginine-utilizing mycoplasma, mycoplasma arginini, and of varying levels of arginine in the growth media of the epstein-barr virus (ebv)-containing eb1 and eb3 cell lines, and the ebv-free rpmi 1788 cell line, were studied. l-arginine, at a concentration of 0.1 mm in the growth medium, led to a reduction of the ebv capsid antigen content of the eb1 and eb3 cells lines as determined by indirect immunofluorescence, and m. arginini infection enhanced this reduction. the synthesis ... | 1974 | 4362772 |
| serological comparison of five arginine-utilizing mycoplasma species by two-dimensional immunoelectrophoresis. | five arginine-utilizing, nonglycolytic mycoplasma species were compared serologically by two dimensional immunoelectrophoresis. the survey included: mycoplasma hominis, mycoplasma arthritidis, mycoplasma gateae, mycoplasma gallinarum, and three strains of mycoplasma arginini. although the m. arginini strains showed strong cross-reactions, each strain produced a different antigenic profile which was distinct and which indicated significant antigenic differences between strains. the m. arginini st ... | 1974 | 4473427 |
| isolation and characterization of mycoplasma arginini: spec. nov. | | 1968 | 4880321 |
| isolation of mycoplasma arginini from commercial bovine sera and its implication in contaminated cell cultures. | | 1971 | 5000781 |
| identification and characterization of mycoplasma arginini from bighorn sheep (ovis canadensis) and goats. | | 1972 | 5058544 |
| the isolation of mycoplasma arginini from captive wild cats. | | 1972 | 5080201 |
| the isolation of mycoplasma arginini from calves with pneumonia in czechoslovakia. | | 1971 | 5168550 |
| the occurrence of mycoplasma arginini in several animal hosts. | | 1970 | 5528579 |
| interaction of feline sarcoma virus (fesv) and mycoplasma. | a factor present in the supernatant of an established human glioma cell line u-251 mg strongly suppresses feline sarcoma virus (fesv) focus forming activity on feline embryo fibroblasts. the factor was identified as mycoplasma arginini. the enriched mycoplasma fraction had no cytpathogenic effect on the glioma cells or on the embryonic feline indicator cells. an antiserum prepared against this strain of mycoplasma abolished the inhibition. the exact mechanism is not known but arginine depletion ... | 1981 | 6274139 |
| lymphokine-like activity of a strain of mycoplasma arginini. | a mycoplasma arginini strain, found to contaminate a t-t hybridoma designated tuh-14, was the source of a lymphokine-like activity with an ability to stimulate b-blasts to proliferate. maturation to immunoglobulin secretion induced by the mycoplasma alone was low compared with induction by lipopolysaccharide (lps), but could reach the same levels achieved with lps by the addition of a b-cell maturation factor obtained from lectin-activated el-4 thymoma cells. the mitogenic effect on b-cells was ... | 1984 | 6334670 |
| lack of correlation between mycoplasma induced ifn-gamma production in vitro and natural killer cell activity against fld-3 cells. | interferon (ifn) stimulates natural killer (nk) cell-mediated lysis of tumor cells. however, it is not clear whether ifn production is essential for nk cells to lyse their target cells in vitro, especially in long-term (greater than 18 hrs) assays. to investigate this, 0.5 x 10(6) normal mouse spleen cells were cocultured in rpmi 1640 medium with friend erythroleukemia cells (fld-3) (1 x 10(4] for 24 hours under conditions which cause lysis of fld-3 cells. supernatant fluid from such cultures de ... | 1983 | 6420333 |
| comparison of virulence of ovine respiratory mycoplasmas in the mouse mammary gland. | the virulence of isolates of mycoplasma ovipneumoniae and m. arginini from pneumonic and unaffected ovine lungs was compared in a mouse mammary gland model. the isolates varied in their ability to induce a neutrophilic response in the mammary gland. a moderate to severe form of mastitis was induced by 3 m. ovipneumoniae isolates recovered from pneumonic lungs, while the remaining m. ovipneumoniae isolates from pneumonic lungs and those from unaffected lungs induced a very mild histopathological ... | 1984 | 6541843 |
| cyanide insensitive respiration in mammalian cells: an artifact of mycoplasmal contamination. | oxygen consumption by mammalian cells occurs primarily in the mitochondria although alternate oxidation pathways are also present. the resistance (often high) of these alternate pathways to metabolic inhibitors like cn- determine the residual oxygen consumption in the presence of the inhibitor. in many plant cells and micro-organisms the cyanide insensitive pathway can account for an appreciable fraction of the total oxygen consumption. we were studying oxidation by electron-affinic drugs in non ... | 1983 | 6637622 |
| the isolation of mycoplasma arginini from a pituitary abscess in a goat. | | 1983 | 6651691 |
| synthesis of adenylate nucleotides by mollicutes (mycoplasmas). | cultures of the mollicutes (mycoplasma) acholeplasma laidlawii b, acholeplasma morum, mycoplasma bovis, mycoplasma arginini, mycoplasma fermentans and mycoplasma gallisepticum, representing four metabolic groups, were sampled at intervals over a 40 to 50 h period and assayed for the numbers of c.f.u., changes in ph and glucose concentration, and concentrations of atp, adp, amp, lactate and pyruvate. the adenylate energy charge (eca), the mean generation time, and the number of nmol of atp (mg dr ... | 1983 | 6655457 |
| elimination of mycoplasmas from cell cultures and establishment of mycoplasma-free cell lines. | several antibiotics were examined for their potential to eliminate mycoplasmas from contaminated cell cultures. acholeplasma laidlawii, mycoplasma arginini, mycoplasma hyorhinis and mycoplasma orale were effectively eliminated from experimentally contaminated mouse fibroblasts and mink epithelial cells by the use of the antibiotics minocycline and tiamutin . an elimination procedure was established, which involved the consecutive treatment of the cultures over a period of 3 weeks, followed by ce ... | 1984 | 6723803 |
| detection of mycoplasmas infecting cell cultures by dna hybridization. | infection of cell cultures by mycoplasmas can be detected and the mycoplasma identified by southern blot hybridization of the eco ri-digested dna of the suspected cell cultures with a nick-translated probe consisting of cloned ribosomal rna genes of mycoplasma capricolum. the probe does not hybridize with eukaryotic dna. the hybridization pattern with mycoplasmal dna is species specific, enabling the identification of the four most prevalent mycoplasma contaminants, mycoplasma orale, mycoplasma ... | 1984 | 6724620 |
| counterimmunoelectrophoresis for the diagnosis of bovine mycoplasmosis. | counterimmunoelectrophoresis (cie) procedure has been established for mycoplasma identification from cultures, and the specificity and the sensitivity of this technique were evaluated. preparations of milk samples and clinical and necropsy specimens were used for the technique. from 171 milk samples, 80 (46.8%) were positive by cie (73 samples with mycoplasma bovis and 7 with mycoplasma arginini) and 77 (45%) were positive by biochemical and growth inhibition tests (70 samples with mycoplasma bo ... | 1984 | 6732006 |
| influence of mycoplasma arginini infection on the induction of aryl hydrocarbon hydroxylase by tcdd in rat hepatoma cell cultures. | mycoplasma arginini was eliminated from a rat hepatoma cell line (h-4-ii-e) by plating at low cell density and treatment with chlortetracycline (250 micrograms/ml), kanamycin (250 micrograms/ml), tylosin (100 micrograms/ml), 3% m. arginini antiserum and 5% fresh guinea-pig serum. the induction of ahh activity in the cell culture was measured in response to increasing concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd). the ed50 values (estimated doses that produce 50% maximum enzyme ind ... | 1982 | 6890515 |
| prevalence of mycoplasma and chlamydia in the nasal flora of dairy calves. | nasal swabs from dairy calves in 20 selected herds were collected once in each of three sampling periods in one year and cultured for mycoplasmas and chlamydiae. seventy-seven of 411 calves (19%) and 16 of 20 herds (80%) were positive for mycoplasmas at least once throughout the year, and all of 378 calves sampled from the 20 herds were negative for chlamydiae. thirty-one percent of the isolates were mycoplasma bovirhinis; other species isolated less frequently were mycoplasma bovis, mycoplasma ... | 1982 | 7179720 |
| characterization of the strain-specific and common surface antigens of mycoplasma arginini. | a combination of quantitative immunoelectrophoresis and sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis was used to determine location and molecular weights of surface membrane antigens of four strains of mycoplasma arginini. two major surface antigens were identified for m. arginini by absorption of antiserum with whole cells: one surface antigen was strain specific, electrophoretically fast, and prominently located on the surface, whereas the other surface antigen was common to ... | 1980 | 7216419 |
| respiration-associated components of mollicutes. | no cytochrome pigments were detected by difference (reduced minus oxidized) spectroscopy at liquid nitrogen temperature in whole-cell preparations or membrane fractions of acholeplasma axanthum s273, acholeplasma equifetale n93, acholeplasma granularum bts39, acholeplasma laidlawii b-pg9, acholeplasma modicum pg-49, acholeplasma oculi 19l, mycoplasma arginini g230, mycoplasma arthritidis 07, mycoplasma pneumoniae fh, and mycoplasma pulmonis jb. all ten mollicutes species examined contained iron ... | 1981 | 7240087 |
| cholesterol is required to prevent crystallization of mycoplasma arginini phospholipids at physiological temperature. | | 1981 | 7308469 |
| [pasteurella sp. and mycoplasma arginini carriers in healthy sheep in senegal]. | | 1981 | 7346906 |
| methylated bases in mycoplasmal dna. | the dnas of four mycoplasma and one acholeplasma species were found to contain methylated bases. all of the five species contained 6-methyladenine (m6ade), the methylated base characteristic of prokaryotic dna. the extent of methylation of adenine residues in the mycoplasmal dna ranged from 0.2% in mycoplasma capricolum to about 2% in mycoplasma arginini and mycoplasma hyorhinis with intermediate methylation values for mycoplasma orale and acholeplasma laidlawii dnas. about 5.8% of the cytosine ... | 1980 | 7433124 |
| anti-tumor activity of arginine deiminase from mycoplasma argini and its growth-inhibitory mechanism. | two kinds of arginine deiminase (ad, ec 3.5.3.6) were purified from cell extracts of mycoplasma arginini (a-ad) and mycoplasma hominis (h-ad), and their enzymic properties and anti-tumor activities were compared. the a-ad enzyme strongly inhibited the growth of mouse hepatoma cell line mh134 in vitro, and its concentration required for 50% growth inhibition (ic50) was estimated to be about 10 ng/ml. the ic50 value of h-ad against the same cell line was estimated to be about 100 ng/ml, due to its ... | 1995 | 7591961 |
| [a cytogenetic study of the chinese hamster lung cell line v-79 infected with mycoplasma arginini and decontaminated using ciprofloxacin]. | karyotypic variability has been studied in a line of the chinese hamster cells artificially contaminated with mycoplasma arginini. the contaminated cultures differed from mycoplasma-free cells in cell distribution for chromosome number. the frequency of cells with modal chromosome number 21 decreased, while that of cells with 20 chromosomes increased. decontamination of cell culture with ciprofloxacin (10 mg/ml) and a subsequent cultivation of cell in the antibiotic-free medium did not restore t ... | 1994 | 7701619 |
| high-level expression of mycoplasma arginine deiminase in escherichia coli and its efficient renaturation as an anti-tumor enzyme. | the arginine deiminase (ad) gene was cloned from mycoplasma arginini and expressed in the cytosol of escherichia coli as inclusion bodies with an expression level of at least 20% of the total bacterial proteins. the inclusion bodies were solubilized with 6 m guanidine hydrochloride (gdn-hcl) under reducing conditions, in order to avoid incorrect disulfide-bond formation of the recombinant (r-) ad molecules, and renaturation was performed under various refolding conditions. the optimum renaturati ... | 1994 | 7765234 |
| mycoplasma arginini tuh-14 membrane lipoproteins induce production of interleukin-1, interleukin-6, and tumor necrosis factor alpha by human monocytes. | mycoplasma arginini tuh-14 partially purified membrane lipoproteins (tuh-14-pp) directly induce secretion of the cytokines involved in the inflammatory response, namely, interleukin 1 (il-1), tumor necrosis factor alpha, and il-6, by human monocytes cultured in the absence of serum. the biological activity of each cytokine correlates with its immunoreactivity. upon stimulation with either tuh-14-pp or lipopolysaccharide, most tumor necrosis factor alpha and il-6 is secreted in the extracellular ... | 1994 | 7927744 |
| mycoplasma arginini--a new human zoonosis? | | 1994 | 8011852 |
| treatment of mycoplasma contamination in a large panel of cell cultures. | mycoplasmal contamination remains a significant impediment to the culture of eukaryotic cells. for certain cultures, attempts to eliminate the infection are feasible alternatives to the normally recommended disposal of the contaminated culture. here, three antibiotic regimens for mycoplasmal decontamination were compared in a large panel of naturally infected cultures: a 1-wk treatment with the fluoroquinolone mycoplasma removal agent (mra), a 2-wk treatment with the fluoroquinolone ciprofloxaci ... | 1994 | 8069460 |
| characterization and purification of a macrophage-triggering factor produced in mycoplasma arginini-infected l5178y cell cultures. | the supernatant of mycoplasma arginini-infected murine l5178y t lymphoma cell cultures (sn-l51) synergizes with small concentrations of ifn-gamma to activate murine peritoneal, thioglycollate-elicited macrophages (m phi) to exhibit cytostatic activity against tumor cells. treatment of m phi with ifn-gamma and sn-l51 sequentially, but not in the reverse order, activates m phi, which indicates that sn-l51 contains a m phi-triggering factor (mtf). mtf activity could be inhibited by small concentrat ... | 1994 | 8077668 |
| polymorphism in genes for the enzyme arginine deiminase among mycoplasma species. | the extent of restriction fragment length polymorphism in genes for the arginine deiminase enzyme among 28 species of mycoplasmas was assessed by southern blot analysis of dna digested with ecori or taqi nuclease probed with a 725-bp internal fragment of the arginine deiminase gene from mycoplasma arginini. the results indicated unexpected heterogeneity among species of a single genus. | 1993 | 8093359 |
| isolation of mycoplasma hyorhinis and mycoplasma arginini from the ears of pigs with otitis media. | mycoplasmas were isolated from the middle ears and nasal cavities of clinically ill, young pigs that were slaughtered due to an unfavourable prognosis. the isolation rates of mycoplasma hyorhinis were 27 of 43 (62.8 per cent) from the auditory tube, 22 of 43 (51.2 per cent) from the tympanic cavity, and 15 of 25 (60.0 per cent) from the nasal cavity. m arginini was also recovered at rates of 11 of 43 (25.6 per cent) from the auditory tube, 7 of 43 (16.3 per cent) from the tympanic cavity, and 9 ... | 1994 | 8146440 |
| rapid, sensitive pcr-based detection of mycoplasmas in simulated samples of animal sera. | a fast and simple method to detect mycoplasmal contamination in simulated samples of animal sera by using a pcr was developed. the following five mycoplasma species that are major cell culture contaminants belonging to the class mollicutes were investigated: mycoplasma arginini, acholeplasma laidlawii, mycoplasma hyorhinis, mycoplasma orale, and mycoplasma fermentans. after a concentration step involving seeded sera, genus-specific primers were used to amplify a 717-bp dna fragment within the 16 ... | 1994 | 8161186 |
| chemical modification by polyethylene glycol of the anti-tumor enzyme arginine deiminase from mycoplasma arginini. | amino acid-degrading enzymes are known to inhibit the growth of tumor cells in culture by depleting amino acids in the medium. here we demonstrate that arginine deiminase (ec 3.5.3.6) from mycoplasma arginini had stronger growth-inhibitory activity against all 4 kinds of tumor cell lines tested than l-asparaginase and arginase, which are well-known anti-tumor enzymes. next, chemical modification of the arginine deiminase molecule with polyethylene glycol was shown to enhance its potency as an an ... | 1993 | 8276724 |
| metastasis-promoting activity of a novel molecule, ag 243-5, derived from mycoplasma, and the complete nucleotide sequence. | we found that mycoplasma-infected cells have a higher ability to metastasize in vivo than non-mycoplasma-infected cells. to investigate this phenomenon, we obtained a monoclonal antibody, mab 243-5, by immunization with mycoplasma arginini-infected rpmi 4788 cells. this mab recognized a mycoplasmal protein with an mw of 47 kda and completely inhibited the experimental metastasis of m. arginini-infected rpmi 4788 cells using a nude mouse model. using this mab, we purified a molecule called ag 243 ... | 1995 | 8551970 |
| taxonomy of the feline mycoplasmas mycoplasma felifaucium, mycoplasma feliminutum, mycoplasma felis, mycoplasma gateae, mycoplasma leocaptivus, mycoplasma leopharyngis, and mycoplasma simbae by 16s rrna gene sequence comparisons. | the nucleotide sequence of the 16s rrna gene of eight mycoplasmas isolated from felids was determined and used for taxonomic comparisons. a signature nucleotide sequence motif and overall sequence similarity to other mollicutes positioned mycoplasma felifaucium, mycoplasma felis, mycoplasma leocaptivus, mycoplasma leopharyngis, and mycoplasma simbae in the mycoplasma fermentans phylogenetic group of mollicutes. mycoplasma arginini and mycoplasma gateae were positioned in the mycoplasma hominis p ... | 1995 | 8590684 |
| characterisation of mycoplasmas isolated from genital tract infections of sheep in nigeria. | four mycoplasma-like organisms isolated from ewes with mucopurulent vaginal discharge and swollen vulva were characterised. biochemical tests showed three of the isolates to be negative for glucose fermentation and arginine hydrolysis, while the remaining isolate was negative for glucose fermentation but hydrolysed arginine. serological identification using the growth inhibition, growth precipitation and indirect immunofluorescence tests indicated the three similar isolates as mycoplasma bovigen ... | 1995 | 8593416 |
| apoptotic cell death of human t lymphoblastoid cells induced by arginine deiminase. | a growth-inhibitory substance found in the culture of a b-precursor leukemia cell line, nalm-20, was purified from the serum-free culture medium and identified as arginine deiminase derived from mycoplasma arginini (ec 3.5.3.6). arginine deiminase strongly inhibited, in a dose-dependent manner, the growth of human t cells and t lymphoblastoid cell lines, but not that of b-precursor and myeloid cell lines. the addition of l-arginine completely restored the growth of t lymphoblastoid cells that ha ... | 1997 | 9071817 |
| bacteria associated with enzootic pneumonia in goats. | a histological and microbiological study of lung samples from 83 slaughtered goats (33 kids and 50 adults) drawn from a flock with a history of pleuropneumonia caused by mycoplasmas of the m. mycoides group was carried out. a total of 82% (27/33) of kids and 36% (18/50) of adult goats presented pulmonary lesions characteristic of enzootic pneumonia: lesions took the form of bronchointerstitial pneumonia with peribronchial and peribronchiolar proliferation of lymphocytes. microbiological analysis ... | 1997 | 9151536 |
| an improved method for the recovery of mycoplasmas from the external ear canal of goats. | the efficacy of ear canal flushing and ear canal and mouth swabbing methods for the isolation of mycoplasmas was investigated in 39 goats. of the 19 goats positive for mycoplasma spp., 14 (73.7%) were positive with the ear canal flushing method, 4 (21.0%) were positive with both ear canal flushing and mouth swabbing methods, and 1 (5.3%) was positive by the mouth swabbing method. mycoplasma arginini, m. mycoides subsp. mycoides, and m. mycoides subsp. capri were identified by direct immunofluore ... | 1997 | 9211234 |
| inhibition of human peripheral blood mononuclear cell proliferation by streptococcus pyogenes cell extract is associated with arginine deiminase activity. | streptococcus pyogenes (group a streptococcus) cell extracts (ce) have a remarkably powerful and dose-dependent inhibitory effect on antigen, superantigen, or mitogen-stimulated human peripheral blood mononuclear cell (pbmc) proliferation in vitro. purification of the inhibitory component present in s. pyogenes type m5 (manfredo strain) ce by anion-exchange chromatography followed by gel filtration chromatography showed that the inhibitor had an approximate native molecular mass of 100 kda. sodi ... | 1998 | 9632565 |
| occurrence of autoantibodies to cilia in lambs with a 'coughing syndrome'. | a respiratory disease of lambs that has been termed the 'coughing syndrome' has been observed in the mid-western region of the united states of america. mycoplasma ovipneumoniae (m. ovipneumoniae) and mycoplasma arginini (m. arginini) were routinely isolated from the respiratory tract of lambs with this disease. a high level of antibodies reactive with ovine cilia of the upper respiratory tract was detected in the sera from many of the lambs in affected flocks but not in sera of lambs from unaff ... | 1998 | 9730216 |
| thioglycollate-elicited murine macrophages are cytotoxic to mycoplasma arginini-infected yac-1 tumor cells. | macrophages are important components of natural immunity involved in inhibition of tumor growth and destruction of tumor cells. it is known that these cells can be activated for tumoricidal activity by lymphokines and bacterial products. we investigated whether yac-1 tumor cells infected with mycoplasma arginini stimulate nitric oxide (no) release and macrophage cytotoxic activity. thioglycollate-elicited macrophages from male balb/c mice were co-cultured for 20 h with yac-1 tumor cells infected ... | 1998 | 9921279 |